AU2012255121B2 - Methods and compositions for detecting target nucleic acids - Google Patents
Methods and compositions for detecting target nucleic acids Download PDFInfo
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- AU2012255121B2 AU2012255121B2 AU2012255121A AU2012255121A AU2012255121B2 AU 2012255121 B2 AU2012255121 B2 AU 2012255121B2 AU 2012255121 A AU2012255121 A AU 2012255121A AU 2012255121 A AU2012255121 A AU 2012255121A AU 2012255121 B2 AU2012255121 B2 AU 2012255121B2
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- ligation
- target
- probes
- probe
- nucleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161486817P | 2011-05-17 | 2011-05-17 | |
| US61/486,817 | 2011-05-17 | ||
| PCT/US2012/038436 WO2012158967A1 (en) | 2011-05-17 | 2012-05-17 | Methods and compositions for detecting target nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2012255121A1 AU2012255121A1 (en) | 2014-01-16 |
| AU2012255121B2 true AU2012255121B2 (en) | 2017-03-09 |
Family
ID=46168641
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012255121A Active AU2012255121B2 (en) | 2011-05-17 | 2012-05-17 | Methods and compositions for detecting target nucleic acids |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US10066257B2 (OSRAM) |
| EP (1) | EP2710145B1 (OSRAM) |
| JP (1) | JP2014513557A (OSRAM) |
| KR (1) | KR102085242B1 (OSRAM) |
| CN (2) | CN106434871B (OSRAM) |
| AU (1) | AU2012255121B2 (OSRAM) |
| CA (1) | CA2836577C (OSRAM) |
| DK (1) | DK2710145T3 (OSRAM) |
| WO (1) | WO2012158967A1 (OSRAM) |
Families Citing this family (84)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007133703A2 (en) * | 2006-05-10 | 2007-11-22 | Dxterity Diagnostics | Detection of nucleic acid targets using chemically reactive oligonucleotide probes |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| AU2012255121B2 (en) * | 2011-05-17 | 2017-03-09 | Dxterity Diagnostics Incorporated | Methods and compositions for detecting target nucleic acids |
| US9777322B2 (en) | 2011-07-08 | 2017-10-03 | Keygene N.V. | Sequence based genotyping based on oligonucleotide ligation assays |
| USRE50065E1 (en) | 2012-10-17 | 2024-07-30 | 10X Genomics Sweden Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| US9080167B2 (en) * | 2012-11-16 | 2015-07-14 | Covaris, Inc. | System and method for processing paraffin embedded samples |
| JP6484177B2 (ja) * | 2012-12-07 | 2019-03-13 | インヴァイティ コーポレイションＩｎｖｉｔａｅ Ｃｏｒｐｏｒａｔｉｏｎ | 多重核酸検出方法 |
| KR101829668B1 (ko) * | 2013-03-27 | 2018-02-14 | 김성천 | 생체분자와 단일가닥핵산의 결합정보를 생성하기 위한 기준물질 및 핵산칩, 이들의 제조방법 및 이들을 이용한 생체분자 분석방법 및 장치 |
| US10510435B2 (en) | 2013-04-30 | 2019-12-17 | California Institute Of Technology | Error correction of multiplex imaging analysis by sequential hybridization |
| FI2992115T4 (fi) | 2013-04-30 | 2025-05-15 | California Institute Of Technology | Molekyylien limitetty leimaus sekventiaalisella hybridisaatioviivakoodauksella |
| CN105849275B (zh) | 2013-06-25 | 2020-03-17 | 普罗格诺西斯生物科学公司 | 检测样品中生物靶标的空间分布的方法和系统 |
| CA2951561C (en) | 2014-06-10 | 2023-04-18 | Dxterity Diagnostics Incorporated | Devices and methods for collecting and stabilizing biological samples |
| US10870845B2 (en) | 2014-07-01 | 2020-12-22 | Global Life Sciences Solutions Operations UK Ltd | Methods for capturing nucleic acids |
| US11091810B2 (en) * | 2015-01-27 | 2021-08-17 | BioSpyder Technologies, Inc. | Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology |
| US10683534B2 (en) | 2015-01-27 | 2020-06-16 | BioSpyder Technologies, Inc. | Ligation assays in liquid phase |
| US9856521B2 (en) * | 2015-01-27 | 2018-01-02 | BioSpyder Technologies, Inc. | Ligation assays in liquid phase |
| WO2016094148A2 (en) * | 2014-12-11 | 2016-06-16 | General Electric Company | Methods for capturing nucleic acids |
| CN105803055A (zh) * | 2014-12-31 | 2016-07-27 | 天昊生物医药科技（苏州）有限公司 | 一种基于多重循环延伸连接的靶基因区域富集新方法 |
| HK1250245A1 (zh) | 2015-04-10 | 2018-12-07 | Spatial Transcriptomics Ab | 生物样本的空间区别、多重核酸分析 |
| WO2017015097A1 (en) * | 2015-07-17 | 2017-01-26 | Nanostring Technologies, Inc. | Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue |
| EP3325669B1 (en) * | 2015-07-24 | 2023-07-05 | The Johns Hopkins University | Compositions and methods of rna analysis |
| WO2017027620A1 (en) * | 2015-08-10 | 2017-02-16 | Life Technologies Corporation | Devices and methods for laser capture microdissection |
| US12421540B2 (en) | 2016-08-01 | 2025-09-23 | California Institute Of Technology | Sequential probing of molecular targets based on pseudo-color barcodes with embedded error correction mechanism |
| US10451599B2 (en) | 2016-09-02 | 2019-10-22 | Zepto Life Technology, LLC | Methods of isolating and detecting metal ions from proteins |
| WO2019046768A1 (en) * | 2017-08-31 | 2019-03-07 | William Marsh Rice University | SYMBOLIC SEQUENCING OF DNA AND RNA BY SEQUENCE CODING |
| EP4589017A3 (en) | 2017-10-06 | 2025-10-08 | 10x Genomics, Inc. | Rna templated ligation |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| US20220042090A1 (en) * | 2018-09-14 | 2022-02-10 | Cold Spring Harbor Laboratory | PROGRAMMABLE RNA-TEMPLATED SEQUENCING BY LIGATION (rSBL) |
| WO2020094970A1 (fr) * | 2018-11-05 | 2020-05-14 | Centre Henri Becquerel | Methode de diagnostic d'un cancer et kit associe |
| WO2020123318A1 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Resolving spatial arrays using deconvolution |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| CN113631240B (zh) * | 2019-02-14 | 2023-08-08 | 高丽大学校算学协力团 | 从生物样品中提取微泡的方法 |
| JP7344973B2 (ja) * | 2019-03-01 | 2023-09-14 | マーシー バイオアナリティクス， インコーポレイテッド | 標的実体を検出するためのシステム、組成物、及び方法 |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| CN214973877U (zh) | 2019-08-09 | 2021-12-03 | 胡桃钳医疗公司 | 用于形成治疗性多核苷酸的微流体设备及微流体路径装置 |
| CA3153533A1 (en) * | 2019-09-09 | 2021-03-18 | Sherlock Biosciences, Inc. | System |
| CA3154045A1 (en) * | 2019-10-10 | 2021-04-15 | Irvin Mark MODLIN | Compositions, methods and kits for biological sample and rna stabilization |
| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| CA3158080A1 (en) * | 2019-11-26 | 2021-06-03 | Huy Quoc NGUYEN | Compositions, sets, and methods related to target analysis |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| ES2982420T3 (es) | 2019-12-23 | 2024-10-16 | 10X Genomics Inc | Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN |
| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
| US12405264B2 (en) | 2020-01-17 | 2025-09-02 | 10X Genomics, Inc. | Electrophoretic system and method for analyte capture |
| US11702693B2 (en) | 2020-01-21 | 2023-07-18 | 10X Genomics, Inc. | Methods for printing cells and generating arrays of barcoded cells |
| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
| US11732300B2 (en) | 2020-02-05 | 2023-08-22 | 10X Genomics, Inc. | Increasing efficiency of spatial analysis in a biological sample |
| WO2021158925A1 (en) | 2020-02-07 | 2021-08-12 | 10X Genomics, Inc. | Quantitative and automated permeabilization performance evaluation for spatial transcriptomics |
| US12281357B1 (en) | 2020-02-14 | 2025-04-22 | 10X Genomics, Inc. | In situ spatial barcoding |
| US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
| ES2965354T3 (es) | 2020-04-22 | 2024-04-12 | 10X Genomics Inc | Métodos para análisis espacial que usan eliminación de ARN elegido como diana |
| WO2021236929A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
| WO2021237087A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
| WO2021242834A1 (en) | 2020-05-26 | 2021-12-02 | 10X Genomics, Inc. | Method for resetting an array |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4421186B1 (en) | 2020-06-08 | 2025-08-13 | 10X Genomics, Inc. | Methods of determining a surgical margin and methods of use thereof |
| ES2999535T3 (en) | 2020-06-10 | 2025-02-26 | 10X Genomics Inc | Methods for determining a location of an analyte in a biological sample |
| WO2021263111A1 (en) | 2020-06-25 | 2021-12-30 | 10X Genomics, Inc. | Spatial analysis of dna methylation |
| CN111944875A (zh) * | 2020-06-28 | 2020-11-17 | 温州医科大学附属眼视光医院 | 一种防止rna降解的保护方法、保护序列、组合物和试剂盒及其应用 |
| US12209280B1 (en) | 2020-07-06 | 2025-01-28 | 10X Genomics, Inc. | Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis |
| US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
| US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
| US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
| US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
| AU2021358061A1 (en) * | 2020-10-06 | 2023-06-08 | Materials and Machines Corporation of America | Method for detection of rna or dna from biological samples |
| US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
| AU2021409136A1 (en) | 2020-12-21 | 2023-06-29 | 10X Genomics, Inc. | Methods, compositions, and systems for capturing probes and/or barcodes |
| ES2960777T3 (es) * | 2021-03-18 | 2024-03-06 | Genomill Health Oy | Métodos para la cuantificación paralela precisa de ácidos nucleicos en muestras diluidas o no purificadas |
| EP4305196B1 (en) | 2021-04-14 | 2025-04-02 | 10X Genomics, Inc. | Methods of measuring mislocalization of an analyte |
| EP4320271B1 (en) | 2021-05-06 | 2025-03-19 | 10X Genomics, Inc. | Methods for increasing resolution of spatial analysis |
| ES3030033T3 (en) | 2021-06-03 | 2025-06-26 | 10X Genomics Inc | Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis |
| ES3011462T3 (en) | 2021-09-01 | 2025-04-07 | 10X Genomics Inc | Methods for blocking a capture probe on a spatial array |
| WO2023086880A1 (en) | 2021-11-10 | 2023-05-19 | 10X Genomics, Inc. | Methods, compositions, and kits for determining the location of an analyte in a biological sample |
| WO2023102118A2 (en) | 2021-12-01 | 2023-06-08 | 10X Genomics, Inc. | Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis |
| KR20230138683A (ko) * | 2022-03-24 | 2023-10-05 | 전북대학교산학협력단 | 역전사를 사용하지 않는 결찰 방법을 이용한 cDNA 합성 기반 표적 유전자 검출용 조성물 및 다중 결찰 보조 재조합효소 중합효소 증폭 방법 |
| EP4504966A1 (en) * | 2022-04-08 | 2025-02-12 | Illumina, Inc. | Aptamer dynamic range compression and detection techniques |
| WO2024080776A1 (ko) * | 2022-10-14 | 2024-04-18 | 고려대학교 산학협력단 | 비색 검출을 위한 황 함유 프로브 및 핵산 내 황 도입을 통한 효소 활성 가속화 방법 |
| WO2025168224A1 (en) * | 2024-02-08 | 2025-08-14 | Leica Microsystems Cms Gmbh | Marker and method for analysing a biological sample |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010114599A1 (en) * | 2009-04-01 | 2010-10-07 | Dx Terity Diagnostics Inc. | Chemical ligation dependent probe amplification (clpa) |
Family Cites Families (84)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4469863A (en) | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
| US5235033A (en) | 1985-03-15 | 1993-08-10 | Anti-Gene Development Group | Alpha-morpholino ribonucleoside derivatives and polymers thereof |
| CA1323293C (en) | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
| US5216141A (en) | 1988-06-06 | 1993-06-01 | Benner Steven A | Oligonucleotide analogs containing sulfur linkages |
| DE68927373T2 (de) | 1988-06-24 | 1997-03-20 | Amgen Inc., Thousand Oaks, Calif. | Verfahren und mittel zum nachweis von nukleinsäuresequenzen |
| DE68926504T2 (de) | 1988-07-20 | 1996-09-12 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
| US5185243A (en) | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
| US5545522A (en) | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
| EP0439182B1 (en) | 1990-01-26 | 1996-04-24 | Abbott Laboratories | Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
| US5602240A (en) | 1990-07-27 | 1997-02-11 | Ciba Geigy Ag. | Backbone modified oligonucleotide analogs |
| US5386023A (en) | 1990-07-27 | 1995-01-31 | Isis Pharmaceuticals | Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling |
| IL99025A0 (en) | 1990-08-15 | 1992-07-15 | Astra Ab | Novel process for the detection of pathogens using dna probes |
| US5683874A (en) | 1991-03-27 | 1997-11-04 | Research Corporation Technologies, Inc. | Single-stranded circular oligonucleotides capable of forming a triplex with a target sequence |
| DK51092D0 (da) | 1991-05-24 | 1992-04-15 | Ole Buchardt | Oligonucleotid-analoge betegnet pna, monomere synthoner og fremgangsmaade til fremstilling deraf samt anvendelser deraf |
| US5644048A (en) | 1992-01-10 | 1997-07-01 | Isis Pharmaceuticals, Inc. | Process for preparing phosphorothioate oligonucleotides |
| US5346994A (en) | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
| US5514545A (en) | 1992-06-11 | 1996-05-07 | Trustees Of The University Of Pennsylvania | Method for characterizing single cells based on RNA amplification for diagnostics and therapeutics |
| AU684279B2 (en) | 1993-04-12 | 1997-12-11 | Northwestern University | Method of forming oligonucleotides |
| US5681943A (en) | 1993-04-12 | 1997-10-28 | Northwestern University | Method for covalently linking adjacent oligonucleotides |
| US6077668A (en) | 1993-04-15 | 2000-06-20 | University Of Rochester | Highly sensitive multimeric nucleic acid probes |
| US5714320A (en) | 1993-04-15 | 1998-02-03 | University Of Rochester | Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides |
| US5846709A (en) | 1993-06-15 | 1998-12-08 | Imclone Systems Incorporated | Chemical process for amplifying and detecting nucleic acid sequences |
| CA2105364A1 (en) | 1993-09-01 | 1995-03-02 | Eric T. Kool | Stem-loop oligonucleotides containing parallel and antiparallel binding domains |
| US5808036A (en) | 1993-09-01 | 1998-09-15 | Research Corporation Technologies Inc. | Stem-loop oligonucleotides containing parallel and antiparallel binding domains |
| US5824473A (en) | 1993-12-10 | 1998-10-20 | California Institute Of Technology | Nucleic acid mediated electron transfer |
| US5591578A (en) | 1993-12-10 | 1997-01-07 | California Institute Of Technology | Nucleic acid mediated electron transfer |
| US5637684A (en) | 1994-02-23 | 1997-06-10 | Isis Pharmaceuticals, Inc. | Phosphoramidate and phosphorothioamidate oligomeric compounds |
| WO1996015271A1 (en) | 1994-11-16 | 1996-05-23 | Abbott Laboratories | Multiplex ligations-dependent amplification |
| US5674683A (en) | 1995-03-21 | 1997-10-07 | Research Corporation Technologies, Inc. | Stem-loop and circular oligonucleotides and method of using |
| US5780613A (en) | 1995-08-01 | 1998-07-14 | Northwestern University | Covalent lock for self-assembled oligonucleotide constructs |
| EP2368897B1 (en) | 1996-02-09 | 2016-10-19 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| US6218108B1 (en) | 1997-05-16 | 2001-04-17 | Research Corporation Technologies, Inc. | Nucleoside analogs with polycyclic aromatic groups attached, methods of synthesis and uses therefor |
| EP0912761A4 (en) * | 1996-05-29 | 2004-06-09 | Cornell Res Foundation Inc | DETERMINATION OF DIFFERENCES IN THE NUCLEIC ACID SEQUENCE BY MEANS OF A COMBINATION OF LIGASE DETERMINATION AND POLYMERASE CHAIN REACTION |
| WO1997046568A1 (en) | 1996-06-07 | 1997-12-11 | California Institute Of Technology | Nucleic acid mediated electron transfer |
| US6984491B2 (en) | 1996-07-29 | 2006-01-10 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses therefor |
| AU739375B2 (en) | 1996-11-05 | 2001-10-11 | Clinical Micro Sensors, Inc. | Electrodes linked via conductive oligomers to nucleic acids |
| US5922553A (en) | 1996-11-21 | 1999-07-13 | Trustees Of The University Of Pennsylvania | Method of detecting protein by immuno RNA |
| US7090804B2 (en) | 1998-01-27 | 2006-08-15 | Clinical Mirco Sensors, Inc. | Amplification of nucleic acids with electronic detection |
| AU2898299A (en) | 1998-03-04 | 1999-09-20 | Onyx Pharmaceuticals, Inc. | Baculovirus expression system and method for high throughput expression of genetic material |
| AU3671099A (en) | 1998-04-29 | 1999-11-16 | Trustees Of Boston University | Methods and compositions pertaining to pd-loops |
| US7033753B1 (en) | 1999-01-15 | 2006-04-25 | University Of Rochester | Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms |
| US6942771B1 (en) | 1999-04-21 | 2005-09-13 | Clinical Micro Sensors, Inc. | Microfluidic systems in the electrochemical detection of target analytes |
| US6297016B1 (en) | 1999-10-08 | 2001-10-02 | Applera Corporation | Template-dependent ligation with PNA-DNA chimeric probes |
| US6479650B1 (en) | 1999-12-14 | 2002-11-12 | Research Corporation Technologies, Inc. | Fluorescent nucleoside analogs and combinatorial fluorophore arrays comprising same |
| US20050214825A1 (en) * | 2000-02-07 | 2005-09-29 | John Stuelpnagel | Multiplex sample analysis on universal arrays |
| EP1130113A1 (en) | 2000-02-15 | 2001-09-05 | Johannes Petrus Schouten | Multiplex ligation dependent amplification assay |
| AU2001265121A1 (en) | 2000-05-30 | 2001-12-11 | Applera Corporation | Methods for detecting target nucleic acids using coupled ligation and amplification |
| US6887664B2 (en) | 2000-06-06 | 2005-05-03 | Applera Corporation | Asynchronous primed PCR |
| US6511810B2 (en) | 2000-07-03 | 2003-01-28 | Applera Corporation | Polynucleotide sequence assay |
| ATE426041T1 (de) * | 2001-02-27 | 2009-04-15 | Virco Bvba | Zirkulare sonden amplifizierung (cpa) von zirkularisierten nucleinsaure-molekulen |
| WO2002083837A1 (en) | 2001-04-11 | 2002-10-24 | Ptc Therapeutics, Inc. | Methods for identifying small molecules that bind specific rna structural motifs |
| FI20021325A0 (fi) | 2002-07-05 | 2002-07-05 | Valtion Teknillinen | Menetelmä ja reagenssipakkaus yksittäisten polynukleotidien määrän määrittämiseksi |
| JP2006510348A (ja) | 2002-07-18 | 2006-03-30 | ザ・ボード・オブ・トラスティーズ・オブ・ザ・レランド・スタンフォード・ジュニア・ユニバーシティ | 蛍光消光脱離基を用いる化学ライゲーションの検出 |
| US7153658B2 (en) | 2002-09-19 | 2006-12-26 | Applera Corporation | Methods and compositions for detecting targets |
| EP1540003A4 (en) | 2002-09-19 | 2006-06-21 | Applera Corp | METHOD AND COMPOSITION FOR DETECTING TARGETS |
| US20040259105A1 (en) | 2002-10-03 | 2004-12-23 | Jian-Bing Fan | Multiplex nucleic acid analysis using archived or fixed samples |
| ES2442791T3 (es) * | 2002-10-11 | 2014-02-13 | Erasmus Universiteit Rotterdam | Cebadores para la amplificación de ácidos nucleicos para estudios de clonalidad basados en PCR de transposiciones en BCL2-IGH |
| US20040235005A1 (en) | 2002-10-23 | 2004-11-25 | Ernest Friedlander | Methods and composition for detecting targets |
| US20040110134A1 (en) | 2002-12-05 | 2004-06-10 | Wenz H. Michael | Methods for quantitating nucleic acids using coupled ligation and amplification |
| KR100959101B1 (ko) | 2003-02-20 | 2010-05-25 | 삼성전자주식회사 | Ｐｃｒ 반응기 및 ｐｃｒ 반응기의 입구와 출구의 개폐를조절하는 방법 |
| GB0304371D0 (en) | 2003-02-26 | 2003-04-02 | Solexa Ltd | DNA Sequence analysis |
| CA2520811C (en) * | 2003-03-28 | 2013-05-28 | Japan As Represented By Director General Of National Rehabilitation Center For Persons With Disabilities | Method of cdna synthesis |
| US7198900B2 (en) | 2003-08-29 | 2007-04-03 | Applera Corporation | Multiplex detection compositions, methods, and kits |
| EP1680517B1 (en) | 2003-11-04 | 2010-03-31 | Applied Biosystems, LLC | Concatameric ligation products: compositions, methods and kits for same |
| WO2005071412A2 (en) | 2004-01-09 | 2005-08-04 | Applera Corporation | Phosphor particle coded beads |
| US20050208503A1 (en) | 2004-03-16 | 2005-09-22 | Handy Yowanto | Chemical ligation of nucleic acids |
| EP1727913B8 (en) | 2004-03-24 | 2009-08-19 | Applied Biosystems, LLC | Ligation and amplification reactions for determining target molecules |
| WO2006076017A2 (en) | 2004-04-30 | 2006-07-20 | Applera Corporation | Methods and kits for identifying target nucleotides in mixed populations |
| US7745614B2 (en) * | 2004-09-03 | 2010-06-29 | The Board Of Trustees Of The Leland Stanford Junior University | Universal linker compositions for the release or transfer of chemical agents from a polynucleotide |
| US20060223122A1 (en) * | 2005-03-08 | 2006-10-05 | Agnes Fogo | Classifying and predicting glomerulosclerosis using a proteomics approach |
| US20060223066A1 (en) * | 2005-03-29 | 2006-10-05 | Applera Corporation | Methods for normalizing and for identifying small nucleic acids |
| GB2424946A (en) * | 2005-04-05 | 2006-10-11 | Stratec Biomedical Systems Ag | A detection system for substance binding using up-converting fluorescent probes |
| US7799530B2 (en) | 2005-09-23 | 2010-09-21 | Celera Corporation | Genetic polymorphisms associated with cardiovascular disorders and drug response, methods of detection and uses thereof |
| WO2007133703A2 (en) | 2006-05-10 | 2007-11-22 | Dxterity Diagnostics | Detection of nucleic acid targets using chemically reactive oligonucleotide probes |
| GB0808413D0 (en) * | 2008-05-09 | 2008-06-18 | Univ Nottingham | Stabilisation of blood cell conjugates |
| WO2011091393A1 (en) * | 2010-01-25 | 2011-07-28 | Rd Biosciences, Inc. | Self-folding amplification of target nucleic acid |
| US9506112B2 (en) * | 2010-02-05 | 2016-11-29 | Siemens Healthcare Diagnostics Inc. | Increasing multiplex level by externalization of passive reference in polymerase chain reactions |
| ES2623859T3 (es) * | 2010-03-04 | 2017-07-12 | Miacom Diagnostics Gmbh | FISH múltiple mejorada |
| US9609769B2 (en) | 2010-07-29 | 2017-03-28 | Rockwell Automation Technologies, Inc. | System and method for ventilating and isolating electrical equipment |
| AU2012255121B2 (en) * | 2011-05-17 | 2017-03-09 | Dxterity Diagnostics Incorporated | Methods and compositions for detecting target nucleic acids |
| WO2012162161A1 (en) * | 2011-05-20 | 2012-11-29 | Phthisis Diagnostics | Microsporidia detection system and method |
| US9901705B2 (en) | 2013-03-15 | 2018-02-27 | Armour Technologies, Inc. | Medical device curving apparatus, system, and method of use |
| FR3054894A1 (fr) | 2016-08-03 | 2018-02-09 | Stmicroelectronics (Crolles 2) Sas | Dispositif integre photonique a compacite amelioree |
-
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-
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010114599A1 (en) * | 2009-04-01 | 2010-10-07 | Dx Terity Diagnostics Inc. | Chemical ligation dependent probe amplification (clpa) |
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| CN106434871A (zh) | 2017-02-22 |
| CN106434871B (zh) | 2020-08-18 |
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| HK1198548A1 (en) | 2015-05-15 |
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