JP2014501503A - 皮膚細胞分化促進物質のスクリーニング方法 - Google Patents
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Abstract
Description
一般的に、細胞内カルシウム濃度が高いと、皮膚細胞の分化が促進されるものと知られている。この実験は、細胞内カルシウム濃度を異ならせて処理することにより、皮膚細胞分化の程度を変化させた後、DUOX1遺伝子の発現程度を評価することとする。
DharmaconeからDUOX1またはDUOX2遺伝子の発現を阻害するsiRNA(SMARTPOOL)を購入した。
皮膚細胞の分化を促進する物質として知られているドクダミと苦楝皮抽出物を利用して、DUOX1、フィラグリン、ケラチン1およびケラチン10の発現程度を確認した。
Claims (13)
- 皮膚細胞に試験物質を処理するステップ;および
前記ステップの試験物質を処理した皮膚細胞において、DUOX1(Dual oxidase 1)遺伝子(Genebank Accession No.:NM_017434)の相対的発現程度を確認するステップを含む皮膚細胞分化促進物質のスクリーニング方法。 - DUOX1遺伝子の相対的発現程度を確認するステップの後、DUOX1遺伝子の発現程度を増加させる物質を皮膚細胞分化促進物質と判断するステップをさらに含むことを特徴とする、請求項1に記載の皮膚細胞分化促進物質のスクリーニング方法。
- DUOX1遺伝子の相対的発現程度を確認するステップに付加して、
試験物質を処理した皮膚細胞において、フィラグリン(Filaggrin)、ロリクリン(Loricrin)、ケラチン1(Keratine 1)およびケラチン10(Keratin 10)からなる群より選択された一つ以上の遺伝子の相対的発現程度を確認するステップをさらに含むことを特徴とする、請求項1に記載の皮膚細胞分化促進物質のスクリーニング方法。 - フィラグリン、ロリクリン、ケラチン1およびケラチン10からなる群より選択された一つ以上の遺伝子の相対的発現程度を確認するステップの後、前記選択された一つ以上の遺伝子の発現程度を増加させる物質を皮膚細胞分化促進物質と判断するステップをさらに含むことを特徴とする、請求項3に記載の皮膚細胞分化促進物質のスクリーニング方法。
- 前記皮膚細胞は、皮膚角質細胞であることを特徴とする、請求項1に記載の皮膚細胞分化促進物質のスクリーニング方法。
- スクリーニング方法は、皮膚細胞分化促進による皮膚保湿用物質のスクリーニング方法であることを特徴とする、請求項1〜5のいずれか1項に記載のスクリーニング方法。
- スクリーニング方法は、皮膚細胞分化促進による皮膚障壁機能強化用物質のスクリーニング方法であることを特徴とする、請求項1〜5のいずれか1項に記載のスクリーニング方法。
- スクリーニング方法は、皮膚細胞分化促進によるアトピー症状軽減または治療用物質のスクリーニング方法であることを特徴とする、請求項1〜5のいずれか1項に記載のスクリーニング方法。
- 皮膚細胞におけるDUOX1遺伝子の相対的発現程度を確認することができる装置を含む、皮膚細胞分化促進物質のスクリーニングキット。
- DUOX1遺伝子の発現を増加させる物質を有効成分として含む皮膚細胞分化促進用組成物。
- DUOX1遺伝子の発現を増加させる物質を有効成分として含む皮膚保湿用組成物。
- DUOX1遺伝子の発現を増加させる物質を有効成分として含む皮膚障壁機能強化用組成物。
- DUOX1遺伝子の発現を増加させる物質を有効成分として含むアトピー症状軽減または治療用組成物。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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KR1020100115853A KR101785347B1 (ko) | 2010-11-19 | 2010-11-19 | 피부 세포 분화 촉진 물질의 스크리닝 방법 |
KR10-2010-0115853 | 2010-11-19 | ||
PCT/KR2011/008838 WO2012067460A2 (ko) | 2010-11-19 | 2011-11-18 | 피부 세포 분화 촉진 물질의 스크리닝 방법 |
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JP2014501503A true JP2014501503A (ja) | 2014-01-23 |
JP6073238B2 JP6073238B2 (ja) | 2017-02-01 |
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US (1) | US20130236904A1 (ja) |
JP (1) | JP6073238B2 (ja) |
KR (1) | KR101785347B1 (ja) |
CN (1) | CN103443291B (ja) |
HK (1) | HK1187077A1 (ja) |
WO (1) | WO2012067460A2 (ja) |
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JP2019530451A (ja) * | 2016-09-28 | 2019-10-24 | アモーレパシフィック コーポレーションAmorepacific Corporation | 皮膚バリア機能診断用バイオマーカー組成物 |
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KR102027451B1 (ko) | 2012-11-02 | 2019-10-02 | (주)아모레퍼시픽 | 마이크로 rna 및 이를 사용하는 스크리닝 방법 |
KR102142324B1 (ko) * | 2014-03-28 | 2020-08-07 | (주)아모레퍼시픽 | 미네랄 성분 및 퀴노아 추출물을 포함하는 피부 보습용 화장료 조성물 |
WO2016061071A1 (en) * | 2014-10-14 | 2016-04-21 | Cellular Dynamics International, Inc. | Generation of keratinocytes from pluripotent stem cells and mantenance of keratinocyte cultures |
KR101655384B1 (ko) * | 2015-01-19 | 2016-09-07 | 고려대학교 산학협력단 | 아토피 피부염 유사 3차원 피부조직 모델 및 이를 이용한 아토피 피부염 치료제의 스크리닝 방법 |
MY193460A (en) * | 2015-04-06 | 2022-10-14 | Amorepacific Corp | Composition for diagnosing skin damage caused by fine dust, and composition comprising galangin as active ingredient |
KR102119466B1 (ko) * | 2018-04-12 | 2020-06-08 | (주)아모레퍼시픽 | 미세먼지에 의한 피부각질 형성 세포의 분화 억제를 회복할 수 있는 물질의 스크리닝 방법 및 미세먼지에 의한 피부각질 형성 세포 분화 억제의 회복용 조성물 |
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JP2004067522A (ja) * | 2002-08-01 | 2004-03-04 | Seiemon Okamoto | 動物忌避剤及びその製造方法 |
JP2009001502A (ja) * | 2007-06-19 | 2009-01-08 | Pola Chem Ind Inc | ベシクル及びそれを含有する皮膚外用剤 |
JP2010013409A (ja) * | 2008-07-04 | 2010-01-21 | Yurika Horinouchi | 化粧料 |
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US7119249B2 (en) * | 2002-07-12 | 2006-10-10 | Emory University | Methods and transgenic mouse model for identifying and modulating factors involved in the production of reactive oxygen intermediates |
US20120034613A1 (en) * | 2010-08-03 | 2012-02-09 | Nse Products, Inc. | Apparatus and Method for Testing Relationships Between Gene Expression and Physical Appearance of Skin |
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JP2004067522A (ja) * | 2002-08-01 | 2004-03-04 | Seiemon Okamoto | 動物忌避剤及びその製造方法 |
JP2009001502A (ja) * | 2007-06-19 | 2009-01-08 | Pola Chem Ind Inc | ベシクル及びそれを含有する皮膚外用剤 |
JP2010013409A (ja) * | 2008-07-04 | 2010-01-21 | Yurika Horinouchi | 化粧料 |
Non-Patent Citations (2)
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---|
JPN6015042613; Toxicol. Environ. Health Sci., 2010.8, Vol.2, Issue 3, pp.162-167 * |
JPN6015042616; Biochem. Pharmacol., 2010.3.15 (online), Vol.80, Issue 1, pp.95-103 * |
Cited By (1)
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JP2019530451A (ja) * | 2016-09-28 | 2019-10-24 | アモーレパシフィック コーポレーションAmorepacific Corporation | 皮膚バリア機能診断用バイオマーカー組成物 |
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CN103443291B (zh) | 2016-03-16 |
KR101785347B1 (ko) | 2017-10-17 |
WO2012067460A3 (ko) | 2012-09-07 |
KR20120088895A (ko) | 2012-08-09 |
HK1187077A1 (zh) | 2014-03-28 |
WO2012067460A2 (ko) | 2012-05-24 |
JP6073238B2 (ja) | 2017-02-01 |
CN103443291A (zh) | 2013-12-11 |
US20130236904A1 (en) | 2013-09-12 |
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Free format text: JAPANESE INTERMEDIATE CODE: R250 |
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R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
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