JP2014066607A - Inspection chip and inspection device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an inspection chip and an inspection device, by which it is possible to further precisely identify whether or not the quantitation of specimen is performed appropriately.SOLUTION: In an inspection chip 2, a specimen quantitating section 114 is capable of containing a predetermined amount of specimen 10 introduced inside the inspection chip 2. When the amount of the specimen 10 exceeds the predetermined amount, the specimen 10 overflows from the specimen quantitating section 114, moves through a first path 115 and is stored in a specimen surplus section 116. At least either of the first path 115 or the specimen surplus section 116 is provided with a specimen reaction section 160. The specimen reaction section 160 changes in appearance when the specimen reaction section 160 contacts the specimen 10. A mixture of the quantitated specimen 10, a first reagent 11 and a second reagent 12 is stored in a reservoir section 170.

Description

  The present invention relates to an inspection chip and an inspection apparatus for performing a chemical, medical, or biological inspection of an inspection object.

  Conventionally, an inspection chip for inspecting a biological substance, a chemical substance, or the like is known. For example, the microchip disclosed in Patent Document 1 is an inspection chip having a plurality of sections corresponding to each item in order to perform inspection and analysis of a plurality of items. The sample introduced from the sample introduction port is distributed to each section. The distributed specimen is quantified by the specimen weighing section of each section. The sample quantified by each sample measuring unit is introduced into the detection unit of each section and used for optical measurement of each item. On the other hand, the sample overflowing from the sample measuring section at the time of quantification in each section is stored in the overflow sample storage section. It is possible to specify whether or not each sample measuring unit is filled with the sample by optical measurement of whether or not the sample exists in the overflowing sample storage unit.

JP 2009-109429 A

  In the microchip disclosed in Patent Document 1, when the amount of liquid stored in the overflowing sample storage unit is very small, the sample present in the overflowing sample storage unit may not be detected by optical measurement. In this case, there is a possibility that the sample is not accurately quantified even if the sample is properly quantified in each sample measuring unit. As a result, even if an appropriate measurement result is obtained in each section, there is a possibility that the measurement result is not adopted as an inaccurate measurement result.

  The objective of this invention is providing the test | inspection chip and test | inspection apparatus which can specify more correctly whether the fixed_quantity | quantitative_assay of the sample was performed appropriately.

  In the first aspect of the present invention, a specimen that is a liquid is injected, a centrifugal force is applied by being rotated about a predetermined first axis, and a second axis different from the first axis is centered. A test chip in which the direction of the centrifugal force is changed by being rotated, the sample introduced into the test chip can flow in, and a quantitative unit capable of accommodating a predetermined amount of the sample And when the sample exceeding the predetermined amount flows into the quantification unit, the spillover overflowing from the quantification unit can be moved, and a downstream end of the connection channel is provided. A surplus part in which the moved specimen is stored; and a specimen reaction part that is provided in at least one of the connection channel and the surplus part and changes its appearance when it comes into contact with the specimen.

  According to the test chip according to the above aspect, the quantification unit can store a predetermined amount of the sample introduced into the test chip. The sample exceeding the predetermined amount overflows from the quantification unit, moves through the connection channel, and is stored in the surplus unit. At least one of the connection channel and the surplus portion is provided with a sample reaction portion whose appearance changes when it comes into contact with the sample. Therefore, it is possible to more accurately specify whether or not the sample has been properly quantified based on whether or not the appearance of the sample reaction part has changed.

  The analyte reaction part may be formed along the inner surface of the connection channel or the inner surface of the surplus part. In this case, it is possible to reduce the possibility that the movement of the reagent in the connection channel or the surplus part is hindered by the sample reaction part.

  The specimen reaction unit may be a barcode indicating information related to the test chip. In this case, the sample reaction unit not only indicates whether the sample has been properly quantified, but also functions as a barcode. Therefore, it is possible to read and use the barcode information from the specimen reaction unit, and there is no need to separately provide a barcode on the test chip.

  The barcode reaction pattern may be formed in the specimen reaction unit by a substance that dissolves upon contact with the specimen. In this case, if the sample is properly quantified, the sample overflowing from the quantification unit comes into contact with the sample reaction unit, and the barcode pattern disappears from the sample reaction unit. Therefore, whether or not the sample is properly quantified can be specified based on whether or not the barcode information can be read.

  The specimen reaction unit may be formed of a substance that causes a color change that can be optically measured when contacting the specimen. In this case, when the sample is properly quantified, the sample overflowing from the quantification unit comes into contact with the sample reaction unit, so that a color change occurs in the sample reaction unit. Therefore, whether or not the sample is properly quantified can be specified based on whether or not a color change has occurred in the sample reaction part.

  The sample reaction part may be provided on a surface connecting the quantification part and the surplus part by the shortest distance among the inner surfaces of the connection channel. In this case, the surface connecting the quantification part and the surplus part with the shortest distance in the connection channel is likely to come into contact with the specimen moving from the quantification part to the surplus part. Therefore, it is possible to more reliably specify whether or not the sample is properly quantified.

  The sample reaction part may be provided on an extension line of the connection channel in the inner surface of the surplus part. In this case, on the extension line of the connection channel in the surplus part, the specimen moving from the quantification part to the surplus part is likely to come into contact. Therefore, it is possible to more reliably specify whether or not the sample is properly quantified.

  A second aspect of the present invention is a test apparatus that can use a test chip into which a sample and a reagent that are liquids are injected, and the test chip that is introduced into the test chip can flow into the test chip. And a quantification unit capable of accommodating a predetermined amount of the sample, and when the sample exceeding the predetermined amount has flowed into the quantification unit, a connecting flow path through which the sample overflowing from the quantification unit can move, Provided at the downstream end of the connection channel and provided in at least one of the surplus part for storing the sample that has moved through the connection channel, the connection channel and the surplus part, and the appearance when contacted with the sample A specimen reaction unit that changes, a specimen that is quantified by the quantification part, and a storage part that stores a mixed solution of the reagent introduced into the test chip. Against the light A measurement unit capable of performing a physical measurement, a rotation unit capable of rotating the test chip around a predetermined first axis and a second axis different from the first axis, and the sample reaction unit by the measurement unit. A first measuring unit that performs optical measurement, a liquid mixing unit that generates the mixed liquid by rotating the inspection chip by the rotating unit after optical measurement is performed by the first measuring unit, and the liquid mixing unit After the mixed liquid is generated by the means, based on the second measurement means for optically measuring the sample reaction part by the measurement part, the measurement result of the first measurement means, and the measurement result of the second measurement means And reaction specifying means for specifying whether or not the appearance of the sample reaction unit has changed.

  According to the inspection apparatus according to the above aspect, it is possible to use an inspection chip into which a specimen and a reagent that are liquids are injected. In the test chip, the quantification unit can accommodate a predetermined amount of the sample introduced into the test chip. The sample exceeding the predetermined amount overflows from the quantification unit, moves through the connection channel, and is stored in the surplus unit. At least one of the connection channel and the surplus portion is provided with a sample reaction portion whose appearance changes when it comes into contact with the sample. The quantified liquid mixture of the sample and the reagent is stored in the storage unit.

  The inspection apparatus optically measures the sample reaction part, and then generates a mixed liquid by rotating the inspection chip. Thereafter, the inspection apparatus optically measures the specimen reaction part again, and determines whether or not the appearance of the specimen reaction part has changed based on the two measurement results. Therefore, it is possible to more accurately specify whether or not the sample has been properly quantified based on whether or not the appearance of the sample reaction part has changed.

It is a rear view of the inspection apparatus 1 in which the inspection chip 2 is in a steady state. It is the other rear view of the inspection apparatus 1 which the test | inspection chip 2 rotated from the steady state. It is a top view of the inspection apparatus 1 shown in FIG. 3 is a block diagram showing an electrical configuration of a control device 90. FIG. It is a perspective view of the test | inspection chip 2. FIG. It is a front view of the test | inspection chip 2 before a centrifugation process. 4 is an enlarged front view of a first passage 115 and a specimen surplus portion 116. FIG. 4 is a flowchart of main processing of the inspection apparatus 1. FIG. 7 is a front view of the inspection chip 2 optically measured at a rotation angle of 60 degrees following FIG. 6. FIG. 10 is a front view of the inspection chip 2 revolved at a rotation angle of 0 degree following FIG. 9. FIG. 11 is a front view of the inspection chip 2 revolved at a rotation angle of 90 degrees following FIG. 10. FIG. 12 is a front view of the inspection chip 2 revolved at a rotation angle of 0 degree following FIG. 11. FIG. 13 is a front view of the inspection chip 2 revolved at a rotation angle of 90 degrees following FIG. 12. FIG. 14 is a front view of the inspection chip 2 optically measured at a rotation angle of 60 degrees following FIG. 13. FIG. 15 is a front view of the inspection chip 2 optically measured at a rotation angle of 0 degree following FIG. 14. It is an expansion perspective view of the 1st passage 115 and sample surplus part 116 concerning a modification.

  Embodiments of the present invention will be described with reference to the drawings. The drawings to be referred to are used to explain technical features that can be adopted by the present invention, and are merely illustrative examples.

<1. Schematic structure of inspection system 3>
An embodiment of the present invention will be described. A schematic structure of the inspection system 3 will be described with reference to FIGS. 1 and 2. The inspection system 3 of the present embodiment includes an inspection chip 2 that can store a specimen and a reagent that are liquids, and an inspection apparatus 1 that performs an inspection using the inspection chip 2. The inspection apparatus 1 can apply a centrifugal force to the inspection chip 2 by rotation about a vertical axis separated from the inspection chip 2. The inspection apparatus 1 can switch the centrifugal direction that is the direction of the centrifugal force applied to the inspection chip 2 by rotating the inspection chip 2 about the horizontal axis.

<2. Detailed structure of the inspection apparatus 1>
With reference to FIGS. 1-4, the detailed structure of the inspection apparatus 1 is demonstrated. In the following description, the upper, lower, right, left, front side, and rear side of FIG. 1 and FIG. 2 are respectively the upper, lower, right, left, rear, and rear sides of the inspection apparatus 1. Let it be in front. The upper, lower, right, left, front side, and back side of FIG. 3 are the front, lower, right, left, upper, and lower sides of the inspection apparatus 1, respectively. In this embodiment, the direction of the vertical axis is the vertical direction, and the direction of the horizontal axis is the direction of the speed when the inspection chip 2 rotates around the vertical axis. For ease of understanding, FIGS. 1 and 2 show the upper housing 30 by phantom lines, and FIG. 3 shows a state where the top plate of the upper housing 30 is removed.

  As shown in FIGS. 1 to 3, the inspection apparatus 1 includes an upper housing 30, a lower housing 31, a turntable 33, an angle changing mechanism 34, and a control device 90. The turntable 33 is a disk-shaped rotating body provided on the upper surface side of the lower housing 31. The inspection chip 2 is held above the turntable 33. The angle changing mechanism 34 is a drive mechanism provided on the turntable 33. This drive mechanism rotates the inspection chip 2 around the horizontal axis. The upper housing 30 is fixed to the upper side of the lower housing 31, and the measurement unit 7 that performs optical measurement on the inspection chip 2 is provided inside. The control device 90 is a controller that controls various processes of the inspection device 1.

  The detailed structure of the lower housing 31 will be described. As shown in FIGS. 1 and 2, the lower housing 31 has a box-like frame structure in which frame members are combined. An upper plate 32 that is a rectangular plate material is provided on the upper surface of the lower housing 31. A turntable 33 is rotatably provided above the upper plate 32. A drive mechanism for rotating the turntable 33 around the vertical axis is provided in the lower housing 31 as follows.

  A spindle motor 35 that supplies a driving force for rotating the turntable 33 is installed on the left side in the lower housing 31. A shaft 36 of the main shaft motor 35 protrudes upward, and a pulley 37 is fixed. A vertical main shaft 57 extending upward from the inside of the lower housing 31 is provided at the center of the lower housing 31. The main shaft 57 passes through the upper plate 32 and protrudes above the lower housing 31. The upper end portion of the main shaft 57 is connected to the center portion of the turntable 33.

  The main shaft 57 is rotatably held by a support member 53 provided immediately below the upper plate 32. A pulley 38 is fixed to the main shaft 57 below the support member 53. A belt 39 is stretched over the pulleys 37 and 38. When the main shaft motor 35 rotates the shaft 36, driving force is transmitted to the main shaft 57 via the pulley 37, the belt 39, and the pulley 38. At this time, the turntable 33 rotates around the main shaft 57 in conjunction with the rotation of the main shaft 57.

  A guide rail 56 extending in the vertical direction inside the lower housing 31 is provided on the right side in the lower housing 31. The T-shaped plate 48 is movable in the vertical direction in the lower housing 31 along the guide rail 56. A groove 80 that is long in the left-right direction is formed on the front side of the T-shaped plate 48, that is, on the back side in FIG. 1 and FIG.

  The aforementioned main shaft 57 is a cylindrical body having a hollow inside. The inner shaft 40 is a shaft that can move in the vertical direction inside the main shaft 57. An upper end portion of the inner shaft 40 passes through the main shaft 57 and is connected to a rack gear 43 described later. A bearing 41 is provided at the left end of the T-shaped plate 48. Inside the bearing 41, the lower end portion of the inner shaft 40 is rotatably held.

  A stepping motor 51 for moving the T-shaped plate 48 up and down is fixed in front of the T-shaped plate 48. The shaft 58 of the stepping motor 51 protrudes rearward, that is, toward the front side of the page in FIGS. A disc-shaped cam plate 59 is fixed to the tip of the shaft 58. A cylindrical projection 70 is provided on the rear surface of the cam plate 59. The tip of the protrusion 70 is inserted into the groove 80 described above. The protrusion 70 can slide in the groove 80. When the stepping motor 51 rotates the shaft 58, the projection 70 moves up and down in conjunction with the rotation of the cam plate 59. At this time, the T-shaped plate 48 moves up and down along the guide rail 56 in conjunction with the protrusion 70 inserted in the groove 80.

  The detailed structure of the angle changing mechanism 34 will be described. The angle changing mechanism 34 has a pair of L-shaped plates 60 fixed to the upper surface of the turntable 33. Each L-shaped plate 60 extends upward from a base portion fixed in the vicinity of the center of the turntable 33, and its upper end portion extends outward in the radial direction of the turntable 33. A rack gear 43 fixed to the inner shaft 40 is provided between the pair of L-shaped plates 60. The rack gear 43 is a metal plate-like member that is long in the vertical direction, and gears are respectively carved on both end faces.

  On the front end side in the extending direction of each L-shaped plate 60, a horizontal support shaft 46 having a gear 45 is rotatably supported. The support shaft 46 is fixed to the inspection chip 2 via a mounting holder (not shown). For this reason, the inspection chip 2 also rotates around the support shaft 46 in conjunction with the rotation of the gear 45. Between the gear 45 and the rack gear 43, a pinion gear 44 supported by an L-shaped plate 60 so as to be rotatable about a horizontal axis is interposed. The pinion gear 44 meshes with the gear 45 and the rack gear 43, respectively. In conjunction with the vertical movement of the rack gear 43, the pinion gear 44 and the gear 45 are driven to rotate, and the inspection chip 2 rotates about the support shaft 46.

  In the present embodiment, as the main shaft motor 35 rotates and drives the turntable 33, the inspection chip 2 rotates around the main shaft 57, which is a vertical axis, and centrifugal force is applied to the inspection chip 2. The rotation around the vertical axis of the inspection chip 2 is called revolution. On the other hand, as the stepping motor 51 moves the inner shaft 40 up and down, the inspection chip 2 rotates around the support shaft 46 which is a horizontal axis, and the direction of the centrifugal force acting on the inspection chip 2 changes relatively. . The rotation around the horizontal axis of the inspection chip 2 is called rotation.

  As shown in FIG. 1, when the T-shaped plate 48 is lowered to the lowermost end of the movable range, the rack gear 43 is also lowered to the lowermost end of the movable range. At this time, the inspection chip 2 is in a steady state where the rotation angle is 0 degree. As shown in FIG. 2, when the T-shaped plate 48 is raised to the uppermost end of the movable range, the rack gear 43 is also raised to the uppermost end of the movable range. At this time, the test | inspection chip 2 will be in the state rotated 180 degree | times centering on the horizontal axis line from a steady state. That is, in this embodiment, the angle width that the test chip 2 can rotate is the rotation angle of 0 degrees to 180 degrees.

  The detailed structure of the upper housing 30 will be described. As shown in FIG. 3, the upper housing 30 has a box-like frame structure in which frame members are combined, and is installed on the upper left side of the upper plate 32. More specifically, the upper housing 30 is provided outside the range in which the inspection chip 2 is rotated as viewed from the main shaft 57 at the rotation center of the turntable 33.

  The measurement unit 7 provided inside the upper housing 30 includes a light source 71 that emits measurement light, and an optical sensor 72 that detects the measurement light emitted from the light source 71. The light source 71 and the optical sensor 72 are disposed on both the front and rear sides of the turntable 33 outside the rotation range of the inspection chip 2. In the present embodiment, the position on the left side of the main shaft 57 in the reciprocable range of the inspection chip 2 is the measurement position at which the inspection chip 2 is irradiated with the measurement light. When the inspection chip 2 is at the measurement position, the measurement light connecting the light source 71 and the optical sensor 72 intersects the front and rear surfaces of the inspection chip 2 substantially perpendicularly.

  The electrical configuration of the control device 90 will be described with reference to FIG. The control device 90 includes a CPU 91 that performs main control of the inspection device 1, a RAM 92 that temporarily stores various data, and a ROM 93 that stores a control program. Connected to the CPU 91 are an operation unit 94 for a user to input instructions to the control device 90, a hard disk device 95 for storing various data and programs, and a display 96 for displaying various information.

  Further, a revolution controller 97, a rotation controller 98, and a measurement controller 99 are connected to the CPU 91. The revolution controller 97 controls the revolution of the inspection chip 2 by transmitting a control signal for rotating the spindle motor 35 to the spindle motor 35. The rotation controller 98 controls the rotation of the inspection chip 2 by transmitting a control signal for rotating the stepping motor 51 to the stepping motor 51. The measurement controller 99 performs optical measurement of the inspection chip 2 by driving the measurement unit 7. Specifically, the measurement controller 99 transmits a control signal for executing light emission of the light source 71 and light detection of the optical sensor 72 to the light source 71 and the optical sensor 72.

<3. Structure of inspection chip 2>
The detailed structure of the test chip 2 according to the present embodiment will be described with reference to FIGS. In the following description, the upper, lower, lower left, upper right, lower right, and upper left in FIG. 5 are the upper, lower, front, rear, right, and left of the test chip 2, respectively. 6 and 7 show front views of the inspection chip 2 with the sheet 29 removed. The same applies to FIGS. 9 to 15 described later.

  As shown in FIG. 5, the inspection chip 2 has a square shape when viewed from the front as an example, and mainly includes a transparent synthetic resin plate 20 having a predetermined thickness. The front surface of the plate member 20 is sealed with a sheet 29 made of a transparent synthetic resin thin plate. Between the plate member 20 and the sheet 29, a liquid flow path 25 is formed through which the liquid sealed in the inspection chip 2 can flow. The liquid flow path 25 is a recess formed on the front side of the plate member 20 with a predetermined depth, and extends in a direction orthogonal to the front-rear direction, which is the thickness direction of the plate member 20. That is, the sheet 29 seals the flow path forming surface of the plate material 20.

  The liquid channel 25 includes a sample injection unit 111, a sample determination unit 114, a sample surplus unit 116, a reagent injection unit 131, a reagent determination unit 134, a reagent surplus unit 136, a reagent injection unit 151, a reagent determination unit 154, and a reagent surplus unit 156. And the storage part 170 are included. As shown in FIG. 6, the specimen injection part 111 is a part into which the specimen 10 is injected and stored, and is a recess that opens upward. The specimen 10 of this embodiment is blood, for example. The reagent injection part 131 is a part into which the first reagent 11 is injected and stored, and is a recess that opens upward. The reagent injection part 151 is a part into which the second reagent 12 is injected and stored, and is a recess that opens upward.

  As shown in FIG. 6, the sample injection unit 111, the reagent injection unit 131, and the reagent injection unit 151 are arranged in the left-right direction on the front surface of the plate member 20 along the upper side portion 21 that is the upper wall surface of the test chip 2. Is formed. Of the sample injection unit 111, the reagent injection unit 131, and the reagent injection unit 151, the sample injection unit 111 is closest to the left side portion 23 that is the left wall surface of the test chip 2. The reagent injection part 151 is closest to the right side part 22 which is the right wall surface of the test chip 2. The reagent injection part 131 is located between the sample injection part 111 and the reagent injection part 151. The lower wall surface of the inspection chip 2 is the lower side portion 24.

  Although not shown, the sheet 29 has a sample injection hole for injecting the sample 10 into the sample injection unit 111. For example, the specimen 10 accommodated in an instrument (not shown) may be injected from the specimen injection hole by a user operation. That is, the sample 10 may be injected into the sample injection unit 111 through the reagent injection hole using a known method. Similarly, although not shown, the sheet 29 is formed with a reagent injection hole for injecting the first reagent 11 into the reagent injection part 131 and a reagent injection hole for injecting the second reagent 12 into the reagent injection part 151. ing. These injection holes may have a shape in which, for example, the upper side portion 21 is open.

  The sample supply path 112 is a flow path of the sample 10 that extends downward from the upper right part of the sample injection unit 111. The lower end portion of the sample supply path 112 is connected to a sample supply section 113 having a narrow flow path. A sample quantitative unit 114 is provided below the sample supply unit 113. The specimen quantification unit 114 is a part that quantifies the specimen 10, and is a recess that opens upward. By applying a centrifugal force from the sample supply unit 113 toward the inside of the recess of the sample quantification unit 114, the same amount of the sample 10 as the volume inside the recess of the sample quantification unit 114 is quantified.

  The first passage 115 extends leftward and the second passage 117 extends rightward from the part where the sample supply unit 113 and the sample determination unit 114 communicate with each other. The first passage 115 extends to the sample surplus part 116 provided at the lower left of the sample determination unit 114. That is, the first passage 115 is bent when viewed from the front so that the flow path forming direction changes. Thereby, in order for the sample 11 to move between the sample quantification unit 114 and the sample surplus unit 116 in the first passage 115, it is necessary to apply external forces in a plurality of different directions to the sample 10. That is, the specimen 10 stored in the specimen surplus section 116 is unlikely to flow back to the specimen quantitative section 114 via the first passage 115.

  The specimen surplus part 116 is a part where the specimen 10 overflowing from the specimen quantification part 114 is stored, and is a concave part extending rightward from the lower end part of the first passage 115. The second passage 117 extends to a storage unit 170 (described later) provided on the lower right side of the sample determination unit 114. That is, the flow path formation direction of the second passage 117 changes for the same reason as the first passage 115. The definition of the boundary between the first passage 115 and the specimen surplus portion 116 will be described later.

  The reagent supply path 132 is a flow path of the first reagent 11 that extends downward from the upper right portion of the reagent injection part 131. The lower end of the reagent supply path 132 is connected to a reagent supply part 133 having a narrow channel. A reagent quantitative unit 134 is provided below the reagent supply unit 133. The reagent quantification unit 134 is a part for quantifying the first reagent 11 and is a recess opened upward. By applying a centrifugal force from the reagent supply unit 133 toward the inside of the recess of the reagent quantification unit 134, the same amount of the first reagent 11 as the volume inside the recess of the reagent quantification unit 134 is quantified.

  The third passage 135 extends leftward and the fourth passage 137 extends rightward from the portion where the reagent supply unit 133 and the reagent quantitative unit 134 communicate. The third passage 135 extends to the reagent surplus portion 136 provided on the lower left side of the reagent fixed amount portion 134. That is, the flow path formation direction of the third passage 135 changes for the same reason as the first passage 115. The reagent surplus part 136 is a part in which the first reagent 11 overflowing from the reagent quantitative part 134 is stored, and is a concave part extending rightward from the lower end part of the third passage 135. The fourth passage 137 extends to a storage unit 170 (described later) provided below the reagent quantitative unit 134. In other words, the flow path formation direction of the fourth passage 137 changes for the same reason as the first passage 115.

  The reagent supply path 152 is a flow path of the second reagent 12 that extends downward from the upper right portion of the reagent injection part 151. The lower end portion of the reagent supply path 152 is connected to a reagent supply section 153 having a narrow flow path. A reagent quantitative unit 154 is provided below the reagent supply unit 153. The reagent quantification unit 154 is a part that quantifies the second reagent 12, and is a recess that opens upward. By applying a centrifugal force from the reagent supply unit 153 toward the inside of the concave portion of the reagent quantitative unit 154, the second reagent 12 having the same volume as the volume inside the concave portion of the reagent quantitative unit 154 is quantified.

  A fifth passage 155 extends leftward and a sixth passage 157 extends rightward from a portion where the reagent supply unit 153 and the reagent quantitative unit 154 communicate with each other. The fifth passage 155 extends to the reagent surplus portion 156 provided on the lower left side of the reagent fixed amount portion 154. That is, the flow path formation direction of the fifth passage 155 changes for the same reason as the first passage 115. The reagent surplus portion 156 is a portion in which the second reagent 12 overflowing from the reagent fixed amount portion 154 is stored, and is a concave portion extending rightward from the lower end portion of the fifth passage 155. The sixth passage 157 extends to a storage unit 170 (described later) provided on the lower left side of the reagent quantitative unit 154. That is, in the sixth passage 157, the flow path formation direction changes for the same reason as the first passage 115.

  The reservoir 170 is a rectangular recess provided in the lower center portion of the inspection chip 2 and opening upward. In the reservoir 170, the specimen 10 flowing from the second passage 117, the first reagent 11 flowing from the fourth passage 137, and the second reagent 12 flowing from the sixth passage 157 are mixed, and the mixing shown in FIG. Liquid 13 is produced. The generated mixed liquid 13 is optically measured by measurement light passing through the storage unit 170, as will be described later.

  As shown in FIG. 7, the inner surface of the first passage 115 sealed with the sheet 29 includes side surfaces 115A to 115D and a rear surface 115E. The side surface 115A extends in the lower left direction from the upper left end portion 114A of the sample quantitative unit 114. The side surface 115B extends downward from the lower left end 115F of the side surface 115A. The side surface 115C extends in the upper left direction from the lower left end 113A of the sample supply unit 113. The side surface 115D extends downward from the upper left end 115G of the side surface 115C. The lower end 115H of the side surface 115B and the lower end 115J of the side surface 115D are at the same position in the vertical direction. The downstream end 115K of the first passage 115 is formed between the lower end 115H of the side surface 115B and the lower end 115J of the side surface 115D. The rear surface 115E is a surface located most rearward in the first passage 115.

  The inner surface of the specimen surplus portion 116 sealed with the sheet 29 includes side surfaces 116A to 116D and a rear surface 116E. The side surface 116A extends rightward from the lower end 115H of the side surface 115B. The side surface 116B extends downward from the right end portion 116F of the side surface 116A. The side surface 116C extends leftward from the lower end 116G of the side surface 116B. The side surface 116D extends downward from the lower end portion 115J of the side surface 115D and is connected to the left end portion 116H of the side surface 116C. The downstream end portion 115 </ b> K of the first passage 115 described above is a boundary between the specimen surplus portion 116 and the first passage 115. The rear surface 116E is the most rearward surface in the specimen surplus portion 116.

  At least one of the first passage 115 and the specimen surplus part 116 is provided with a specimen reaction part 160 whose appearance changes when it comes into contact with the specimen 10. The sample reaction unit 160 of the present embodiment is a barcode indicating information on the test chip 2 printed on the rear surface 116E of the sample surplus portion 116 with water-soluble dye ink.

  In other words, the specimen reaction unit 160 is a printed matter formed along the inner surface of the specimen surplus part 116. Therefore, the possibility that the movement of the sample 10 in the sample surplus part 116 is hindered by the sample reaction part 160 can be reduced. The specimen reaction unit 160 has a barcode pattern formed of a substance that dissolves when it comes into contact with the specimen 10. Therefore, the specimen reaction unit 160 dissolves when it contacts the liquid specimen 10, and at least a part of the barcode pattern disappears.

  The inspection apparatus 1 can acquire information regarding the inspection chip 2 by reading the sample reaction unit 160. Information about the inspection chip 2 is, for example, device information, chip information, or reagent information. The device information is, for example, the model number, manufacturing number, or driving condition of the inspection device 1 in which the inspection chip 2 is used. The driving condition is information such as the revolution speed. The chip information is, for example, the model number, manufacturing number, or inspection item of the inspection chip 2. The reagent information is, for example, the serial number of the reagent in the test chip 2, the expiration date, correction data, or calibration curve information. The correction data is data for correcting the test result measured from the mixed solution of the reagent and the specimen. The calibration curve information is data for calculating the concentration of the test substance from the absorbance measured from the mixed solution of the reagent and the specimen and the amount of change in absorbance. The barcode pattern of the present embodiment indicates calibration curve information regarding the first reagent 11 and the second reagent 12.

  The sample reaction unit 160 of the present embodiment is provided on the extension line E of the first passage 115 in the inner surface of the sample surplus portion 116. The extension line E is an extension line extending from the downstream side surface 115B into the sample surplus portion 116 among the side surface 115A and the side surface 115B connecting the sample quantification unit 114 and the sample surplus portion 116 with the shortest distance. That is, when the sample 10 flows into the sample surplus portion 116 via the first passage 115, the moving distance of the sample 10 becomes the shortest when the sample 10 moves along the side surface 115A and the side surface 115B. Therefore, the sample 10 moving along the side surface 115A and the side surface 115B is likely to contact the sample reaction unit 160 provided on the extension line E. Further, the upper center portion of the barcode in the specimen reaction unit 160 is located on the extension line E of the side surface 115B. Therefore, when the specimen 10 flows into the specimen surplus portion 116 along the side surface 115B, at least the upper center portion of the barcode is dissolved.

  As shown in FIG. 1, the support shaft 46 extending from the L-shaped plate 60 is vertically connected to the center of the rear surface of the plate member 20 via a mounting holder (not shown). A center Ct illustrated in FIG. 6 is a connection position of the support shaft 46 in the inspection chip 2 and a rotation center of the inspection chip 2. In the present embodiment, the distance L1 from the center Ct to the specimen reaction unit 160 is equal to the distance L2 from the center Ct to the storage unit 170.

  With the rotation of the support shaft 46, the inspection chip 2 rotates in the counterclockwise direction as viewed from the front centering on the center Ct. When the inspection chip 2 is in the steady state shown in FIG. 6, the upper side 21 and the lower side 24 are orthogonal to the direction of the gravity Z, the right side 22 and the left side 23 are parallel to the direction of the gravity Z, and the left side 23 Is disposed closer to the main shaft 57 than the right side portion 22. When the inspection chip 2 in the steady state is arranged at the measurement position, the measurement light connecting the light source 71 and the optical sensor 72 passes vertically through the storage unit 170.

<4. Example of inspection method>
An inspection method using the inspection device 1 and the inspection chip 2 will be described with reference to FIG. 4 and FIGS. The user attaches the inspection chip 2 to the spindle 46 and inputs a process start command from the operation unit 94. Thereby, the CPU 91 executes the main process shown in FIG. 8 based on the control program stored in the ROM 93. Note that the inspection apparatus 1 can inspect two inspection chips 2 at the same time, but the procedure for inspecting one inspection chip 2 will be described below for convenience of explanation.

  As shown in FIG. 8, first, reading of the sample reaction unit 160 is executed (S1). Specifically, the revolution controller 97 shown in FIG. 4 controls the spindle motor 35 to cause the inspection chip 2 to revolve and move to the measurement position. Further, when the rotation controller 98 shown in FIG. 4 controls the stepping motor 51, as shown in FIG. 9, the test chip 2 in the steady state rotates by 60 degrees counterclockwise as an example. As a result, the rotation angle of the inspection chip 2 changes to 60 degrees. At this time, the direction in which the gravity Z acts is directed from the upper right side to the lower left side of the inspection chip 2. Since the specimen injection part 111 is a recess that closes in the lower left direction, the specimen 10 remains without moving from the specimen injection part 111. Since the reagent injection part 131 and the reagent injection part 151 are recessed parts that close in the lower left direction, the first reagent 11 and the second reagent 12 remain without moving from the reagent injection part 131 and the reagent injection part 151, respectively. When the measurement controller 99 drives the measurement unit 7 in this state, measurement light connecting the light source 71 and the optical sensor 72 passes through the sample reaction unit 160.

  As shown in FIG. 7, in the present embodiment, the cross section LH of the measurement light that connects the light source 71 and the optical sensor 72 is a rectangular shape that is long in the vertical direction of the test chip 2. The specimen reaction unit 160 is a barcode formed with a size included in the range of the cross section LH of the measurement light. When the measurement light passes through the colored portion of the barcode, it changes as compared with the case where it passes through the non-colored portion of the barcode. The CPU 91 reads barcode information, which is information related to the test chip 2 indicated by the sample reaction unit 160, based on the change amount of the measurement light received by the optical sensor 72.

  Returning to FIG. It is determined whether or not the barcode information has been read from the sample reaction unit 160 (S3). For example, when the test chip 2 has been used, the bar code information cannot be read because the sample reaction unit 160 is dissolved as will be described later. When the barcode information cannot be read as described above (S3: NO), an error indicating that the barcode information cannot be read is displayed on the display 96 (S5). Thereafter, the main process is terminated. When the barcode information can be read (S3: YES), the calibration curve information indicated by the read barcode information is acquired (S7).

  Next, the centrifuge processing of the inspection chip 2 is executed by the following procedure (S9). First, as shown in FIG. 10, the rotation controller 98 shown in FIG. 4 controls the stepping motor 51 to rotate the inspection chip 2 by 60 degrees clockwise as viewed from the front. The revolution controller 97 shown in FIG. 4 revolves the inspection chip 2 whose rotation angle has returned to 0 degrees by controlling the spindle motor 35. Thereby, the centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22. Due to the action of the centrifugal force X, the specimen 10 moves from the specimen injection part 111 to the specimen supply path 112. Similarly, the first reagent 11 moves from the reagent injection part 131 to the reagent supply path 132. The second reagent 12 moves from the reagent injection part 151 to the reagent supply path 152.

  Next, as shown in FIG. 11, the rotation controller 98 shown in FIG. 4 controls the stepping motor 51 to rotate the revolving test chip 2 counterclockwise by 90 degrees as viewed from the front. As a result, the rotation angle of the inspection chip 2 changes to 90 degrees, and the centrifugal force X acts on the inspection chip 2 from the upper side portion 21 toward the lower side portion 24. Due to the action of the centrifugal force X, the sample 10 flows into the sample quantification unit 114 via the sample supply unit 113. In the sample determination unit 114, the sample 10 exceeding a predetermined amount overflows into the first passage 115 and is stored in the sample surplus unit 116. As a result, a predetermined amount of the specimen 10 is quantified.

  Similarly, the first reagent 11 flows into the reagent quantitative unit 134 via the reagent supply unit 133. In the reagent quantitative unit 134, the first reagent 11 exceeding a predetermined amount overflows into the third passage 135. The overflowed first reagent 11 is stored in the reagent surplus part 136. As a result, a predetermined amount of the first reagent 11 is quantified. The second reagent 12 flows into the reagent quantitative unit 154 via the reagent supply unit 153. In the reagent quantitative unit 154, the second reagent 12 exceeding a predetermined amount overflows into the fifth passage 155. The overflowing second reagent 12 is stored in the reagent surplus portion 156. As a result, a predetermined amount of the second reagent 12 is quantified.

  When the sample 10 overflows from the sample quantitative unit 114 by the above processing, as shown in FIG. 7, the overflowed sample 10 is the shortest from the sample quantitative unit 114 to the sample surplus unit 116 along the side surface 115A and the side surface 115B. Move by distance. Further, the specimen 10 moves on the extension line E along the rear surface 116E from the lower end portion of the side surface 115B to a position where it contacts the side surface 116C. At this time, the sample 10 comes into contact with the sample reaction unit 160 provided on the rear surface 116E, and the sample reaction unit 160 is dissolved.

  Next, as shown in FIG. 12, the rotation controller 98 shown in FIG. 4 controls the stepping motor 51 to rotate the revolving test chip 2 90 degrees clockwise as viewed from the front. As a result, the rotation angle of the inspection chip 2 returns to 0 degrees, and the centrifugal force X acts on the inspection chip 2 from the left side portion 23 toward the right side portion 22. Due to the action of the centrifugal force X, the specimen 10 quantified in the specimen quantification unit 114 moves to the second passage 117. On the other hand, since the specimen surplus portion 116 is a concave portion that closes in the right direction, the surplus specimen 10 remains in the specimen surplus portion 116.

  Similarly, the first reagent 11 quantified by the reagent quantification unit 134 moves to the fourth passage 137. On the other hand, since the reagent surplus portion 136 is a recess that closes in the right direction, the surplus first reagent 11 remains in the reagent surplus portion 136. The second reagent 12 quantified by the reagent quantification unit 154 moves to the sixth passage 157. On the other hand, since the reagent surplus portion 156 is a recess that closes in the right direction, the surplus second reagent 12 remains in the reagent surplus portion 156.

  Next, as shown in FIG. 13, the rotation controller 98 shown in FIG. 4 controls the stepping motor 51 to rotate the revolving inspection chip 2 counterclockwise by 90 degrees as viewed from the front. As a result, the rotation angle of the inspection chip 2 changes to 90 degrees, and the centrifugal force X acts on the inspection chip 2 from the upper side portion 21 toward the lower side portion 24. Due to the action of the centrifugal force X, the specimen 10 flows from the second passage 117 into the reservoir 170. The first reagent 11 flows from the fourth passage 137 into the storage unit 170. The second reagent 12 flows from the sixth passage 157 into the storage unit 170. On the other hand, the surplus specimen 10, the first reagent 11, and the second reagent 12 remain in the specimen surplus part 116, the reagent surplus part 136, and the reagent surplus part 156 as described above. The specimen 10, the first reagent 11, and the second reagent 12 that have flowed into the storage unit 170 are mixed by the action of the centrifugal force X, and the mixed liquid 13 is generated.

  After the centrifugation process is executed, the sample reaction unit 160 is read in the same manner as in step S1 (S11). That is, as shown in FIG. 14, the revolution controller 97 shown in FIG. 4 controls the spindle motor 35 to move the inspection chip 2 to the measurement position. The rotation controller 98 shown in FIG. 4 controls the stepping motor 51 to change the rotation angle of the inspection chip 2 to 60 degrees as an example. At this time, the direction in which the gravity Z acts is directed from the upper right side to the lower left side of the inspection chip 2. Since the reservoir 170 is a recess that closes in the lower left direction, the liquid mixture 13 remains without moving from the reservoir 170. When the measurement controller 99 shown in FIG. 4 drives the measurement unit 7, the measurement light passes through the sample reaction unit 160. The CPU 91 reads the barcode information of the sample reaction unit 160 based on the change amount of the measurement light received by the optical sensor 72.

  Next, it is determined whether or not the appearance of the sample reaction unit 160 has changed from the time of step S1 based on whether or not the barcode information has been read from the sample reaction unit 160 (S13). If the required amount of the sample 10 is not quantified in step S9, the sample 10 does not overflow from the sample quantification unit 114, and therefore the barcode pattern of the sample reaction unit 160 is not dissolved. In this case, since the appearance of the sample reaction unit 160 does not change, the CPU 91 can read the barcode information as in step S1. That is, when the appearance of the sample reaction unit 160 has not changed (S13: NO), since the required amount of the sample 10 has not been quantified, an error notifying the shortage of the sample 10 is displayed on the display 96 (S15). . Thereafter, the main process is terminated.

  On the other hand, when the required amount of the sample 10 is quantified in step S9, the sample 10 overflows from the sample quantification unit 114, so that the barcode pattern of the sample reaction unit 160 is dissolved as described above. That is, since the appearance of the sample reaction unit 160 changes, the CPU 91 cannot read the barcode information. Therefore, when the appearance of the sample reaction unit 160 has changed (S13: YES), since the required amount of the sample 10 has been quantified, optical measurement of the mixed liquid 13 is executed (S17).

  In step S17, as shown in FIG. 15, the rotation controller 98 shown in FIG. 4 controls the stepping motor 51 to change the rotation angle of the test chip 2 to 0 degrees. In this state, when the measurement controller 99 shown in FIG. 4 drives the measurement unit 7, the measurement light connecting the light source 71 and the optical sensor 72 passes through the storage unit 170. The CPU 9 measures the change amount of the measurement light received by the optical sensor 72, that is, the change amount of the measurement light transmitted through the mixed liquid 13. As described above, the distance L1 and the distance L2 are equal. Therefore, the specimen reaction unit 160 and the storage unit 170 can be measured with the same measurement light by rotating the test chip 2.

  Next, the concentration of the test substance is calculated based on the calibration curve information acquired in step S7 and the measurement result acquired in step S9 (S19). The test result of the specimen 10 based on the calculated concentration is displayed on the display 96 shown in FIG. 5 (S21). Thereafter, the main process is terminated.

<5. Main actions and effects of this embodiment>
As described above, according to the test chip 2 and the test apparatus 1 of the present embodiment, the test apparatus 1 can use the test chip 2 into which the specimen 10, the first reagent 11, and the second reagent 12 are injected. It is. In the test chip 2, the sample quantitative unit 114 can store a predetermined amount of the sample 10 introduced into the test chip 2. The specimen 10 exceeding the predetermined amount overflows from the specimen quantification unit 114, moves through the first passage 115, and is stored in the specimen surplus part 116. At least one of the first passage 115 and the specimen surplus part 116 is provided with a specimen reaction part 160 whose appearance changes when it comes into contact with the specimen 10. The mixed liquid 13 of the sample 10, the first reagent 11, and the second reagent 12 quantified is stored in the storage unit 170.

  After optically measuring the sample reaction unit 160, the inspection apparatus 1 generates the mixed liquid 13 by rotating the inspection chip (S1, S3: YES, S7, S9). Thereafter, the test apparatus 1 optically measures the sample reaction unit 160 again, and specifies whether or not the appearance of the sample reaction unit 160 has changed based on the two measurement results (S11, S13). Therefore, whether or not the quantification of the sample 10 is properly performed can be specified more accurately depending on whether or not the appearance of the sample reaction unit 160 has changed.

  The sample reaction unit 160 not only indicates whether or not the sample 10 has been properly quantified, but also functions as a barcode. Therefore, the inspection apparatus 1 can read the barcode information from the sample reaction unit 160 and use it, for example, for inspection (S7, S19). Further, the test chip 2 does not need to provide a bar code indicating information on the test chip 2 separately from the sample reaction unit 160.

  When the sample 10 is properly quantified, the barcode pattern disappears from the sample reaction unit 160. Therefore, the inspection apparatus 1 can specify whether or not the sample has been properly quantified based on whether or not the barcode information has been read (S11, S13).

<6. Other>
In the above embodiment, the sample quantification unit 114 corresponds to the “quantification unit” of the present invention. The first passage 115 corresponds to the “connection channel” of the present invention. The specimen surplus part 116 corresponds to the “surplus part” of the present invention. The first reagent 11 and the second reagent 12 each correspond to a “reagent” of the present invention. The spindle motor 35 and the stepping motor 51 correspond to the “rotating part” of the present invention. The CPU 91 that executes step S1 corresponds to the “first measuring means” of the present invention. The CPU 91 executing step S9 corresponds to the “liquid mixing unit” of the present invention. The CPU 91 executing step S11 corresponds to the “second measuring unit” of the present invention. The CPU 91 executing step S13 corresponds to the “reaction specifying unit” of the present invention.

  The present invention is not limited to the above embodiment, and various modifications can be made. The inspection apparatus 1 and the inspection chip 2 of the above embodiment are merely examples, and the structure, shape, processing, and the like of each can be changed. For example, the specimen reaction unit 160 is not limited to a water-soluble barcode provided on the rear surface 116E of the specimen surplus part 116. The sample reaction unit 160 may change its appearance when it comes into contact with the sample 10. The sample reaction unit 160 may be provided in at least one of the first passage 115 and the sample surplus unit 116.

  In the modification shown in FIG. 16, the sample reaction unit 160A is provided on the upstream side surface 115A among the side surface 115A and the side surface 115B constituting the surface connecting the sample quantification unit 114 and the sample surplus unit 116 with the shortest distance. Yes. The sample reaction unit 160B is provided with a sample reaction unit 160B at a site on the extension line E of the first passage 115 in the side surface 116C. Each of the sample reaction units 160A and 160B is formed in a circular shape by applying a luminol solution that generates a color change that can be optically measured when it comes into contact with blood as the sample 10.

  Also in the case of this modification, the inspection apparatus 1 can perform an inspection using the inspection chip 2 by performing the same process as the main process shown in FIG. However, the testing apparatus 1 according to the modification includes a measurement unit (not shown) for optically measuring at least one of the sample reaction units 160A and 160B from the left side 23 side of the test chip 2. In step S1 and step S11, the CPU 91 reads at least one of the sample reaction units 160A and 160B using a measurement unit (not shown).

  In the main process shown in FIG. 8, the color of the specimen reaction unit 160 is read (S1). When the color of the sample reaction unit 160 cannot be read (S3: NO), a reading error is displayed (S5). When the color of the specimen reaction unit 160 is read (S3: YES), the step S7 is omitted and the centrifugation process is executed (S9). Next, the color of the specimen reaction unit 160 is read again (S11).

  When the necessary amount of the specimen 10 is quantified in step S9, the specimen 10 overflows from the specimen quantification unit 114. When the overflowing specimen 10 comes into contact with the specimen reaction parts 160A and 160B, the specimen reaction parts 160A and 160B cause a color change. CPU91 reads the color different from step S1 from the sample reaction part 160 (S11). In this case, it is determined that the appearance of the sample reaction unit 160 has changed (S13: YES), and optical measurement of the mixed solution 13 is performed (S17). Step S19 is omitted, and the inspection result based on the measurement result of step S17 is displayed (S21).

  If the required amount of the sample 10 is not quantified in step S9, the sample 10 does not overflow from the sample quantification unit 114, so the color of the sample reaction unit 160 does not change. The CPU 91 reads the same color as in step S1 from the sample reaction unit 160 (S11). That is, it is determined that the external appearance of the sample reaction unit 160 has not changed (S13: NO), and an error notifying the shortage of the sample 10 is displayed (S15).

  According to this modification, the specimen reaction units 160A and 160B are formed of a substance that causes a color change that can be optically measured when contacting the specimen 10. When the sample 10 is properly quantified, a color change occurs in the sample reaction units 160A and 160B. Therefore, the test apparatus 1 can specify whether or not the quantification of the sample 10 has been properly performed based on whether or not a color change has occurred in the sample reaction units 160A and 160B.

  The sample reaction unit 160A is provided on at least one of the side surface 115A and the side surface 115B constituting the surface connecting the sample determination unit 114 and the sample surplus unit 116 with the shortest distance among the inner surfaces of the first passage 115. The sample reaction part 160B is provided on the extension line E of the first passage 115 in the inner surface of the sample surplus part 116. On the side surface 115A, the side surface 115B, and the extension line E, the sample 10 moving from the sample quantification unit 114 to the sample surplus unit 116 is likely to come into contact. Therefore, it can be specified more reliably whether or not the sample 10 has been properly quantified.

  In the above embodiment, the specimen reaction unit 160 is not limited to a one-dimensional barcode, and may be a two-dimensional barcode. In the above modification, only one of the specimen reaction units 160A and 160B may be provided. In the embodiment and the modification, the sample reaction unit may be provided at a position where the sample 10 overflowing from the sample determination unit 114 passes through either the first passage 115 or the sample surplus unit 116.

  In the embodiment and the modification, the sample reaction units 160, 160A, and 160B are formed along the inner surface of the first passage 115 or the inner surface of the sample surplus portion 116, but the sample reaction unit is the first passage 115 or the sample surplus. You may provide in the other site | part in the part 116. FIG. For example, the specimen reaction unit may be a water-soluble sheet that closes the flow path of the first passage 115. In this case, when the specimen flows through the first passage 115, the specimen is brought into contact with the water-soluble sheet, so that the sheet is dissolved. The inspection apparatus 1 can specify whether or not the sample 10 has been properly quantified by optically detecting whether or not the water-soluble sheet is dissolved. Further, the sample reaction part may be a solid substance that is accommodated in the sample surplus part 116 and changes its color when it comes into contact with the liquid. In this case, when the specimen flows into the specimen surplus portion 116, the color of the solid matter changes due to the specimen coming into contact with the solid matter. The inspection apparatus 1 can identify whether or not the sample 10 has been properly quantified by optically detecting whether or not a color change has occurred in the solid matter.

  The specimen reaction part is not limited to a size that fits within the range of the cross section LH of the measurement light, and may be any size and shape that can be read by the inspection apparatus 1. For example, the barcode pattern of the specimen reaction unit may be provided along an arcuate locus in which the measurement light relatively moves when the test chip 2 rotates. In this case, the measuring device moves on the sample reaction part with the rotation of the test chip 2, whereby the test apparatus 1 can read the barcode information.

  The barcode pattern of the specimen reaction part is not limited to the case where the whole is dissolved and disappears, but it is sufficient that at least the part read by the measurement light is dissolved and disappears. For example, when the sample reaction part is an arc-shaped bar code as described above, among the bar code patterns of the sample reaction part, it intersects with an arc-shaped trajectory in which the measurement light relatively moves when the test chip 2 rotates. Only the part may dissolve and disappear.

  Although the inspection chip 2 includes the plate material 20 and the sheet 29, the inspection chip 2 may not include the sheet 29. For example, you may use the test | inspection chip 2 in which the liquid flow path 25 was directly formed in the board | plate material 20. FIG. The number of reagents injected into the test chip 2 is not limited to two, and may be one reagent or three or more reagents.

DESCRIPTION OF SYMBOLS 1 Test | inspection apparatus 2 Test | inspection chip 7 Measuring part 10 Sample 11 1st reagent 12 2nd reagent 13 Mixed liquid 35 Spindle motor 51 Stepping motor 91 CPU
114 Sample quantification unit 115 First passage 116 Sample surplus unit 160 Sample reaction unit 160A Sample reaction unit 160B Sample reaction unit 170 Storage unit

Claims (8)

A specimen that is a liquid is injected, and centrifugal force is applied by rotating about a predetermined first axis, and the centrifugal force is rotated by rotating about a second axis different from the first axis. An inspection chip whose direction is changed,
A quantification unit into which the sample introduced into the test chip can flow, and can store a predetermined amount of the sample;
When the sample exceeding the predetermined amount flows into the quantification unit, a connection channel through which the sample overflowing from the quantification unit can move,
A surplus part that is provided at the downstream end of the connection channel and stores the specimen that has moved through the connection channel;
A test chip provided with at least one of the connection channel and the surplus part, and a sample reaction part that changes its appearance when it comes into contact with the sample.   2. The test chip according to claim 1, wherein the specimen reaction part is formed along an inner surface of the connection channel or an inner surface of the surplus part.   The test chip according to claim 2, wherein the sample reaction unit is a barcode indicating information on the test chip.   The test chip according to claim 3, wherein the barcode reaction pattern is formed by a substance that dissolves when the specimen reaction unit comes into contact with the specimen.   The test chip according to claim 2, wherein the specimen reaction unit is formed of a substance that generates a color change that can be optically measured when contacting the specimen.   The said sample reaction part is provided in the surface which connects the said fixed_quantity | quantitative_assay part and the said surplus part by the shortest distance among the inner surfaces of the said connection flow path. Inspection chip.   6. The test chip according to claim 2, wherein the sample reaction part is provided on an extension line of the connection channel in the inner surface of the surplus part. An inspection apparatus capable of using an inspection chip into which a liquid specimen and reagent are injected,
The inspection chip is
A quantification unit into which the sample introduced into the test chip can flow, and can store a predetermined amount of the sample;
When the sample exceeding the predetermined amount flows into the quantification unit, a connection channel through which the sample overflowing from the quantification unit can move,
A surplus part provided at the downstream end of the connection channel and storing the sample that has moved through the connection channel, provided in at least one of the connection channel and the surplus part, and external appearance when in contact with the sample The specimen reaction part where
A reservoir for storing a mixture of the sample quantified by the quantifier and the reagent introduced into the test chip;
The inspection device includes:
A measurement unit capable of performing optical measurement by emitting light to an object;
A rotating part capable of rotating the inspection chip around a predetermined first axis and a second axis different from the first axis;
First measurement means for optically measuring the sample reaction part by the measurement part;
After optical measurement is performed by the first measuring unit, a liquid mixing unit that generates the mixed liquid by rotating the inspection chip by the rotating unit;
Second measurement means for optically measuring the sample reaction part by the measurement part after the liquid mixture is generated by the liquid mixing part;
A test specifying means for specifying whether or not the appearance of the sample reaction part has changed based on the measurement result of the first measurement means and the measurement result of the second measurement means. apparatus.

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