JP6160647B2 - Inspection chip - Google Patents

Description

  The present invention relates to an inspection chip.

  2. Description of the Related Art Conventionally, for example, a testing chip used for testing by an ELISA (ELISA is an enzyme-linked immunosorbent assay) method using an antigen-antibody reaction, such as a disc-shaped analysis chip described in Patent Document 1, is known. Generally, in an ELISA method, an object on which an antibody is immobilized is used. The object on which the antibody is solid-phased is, for example, a part of the flow path in the test chip or a bead arranged in the flow path of the test chip. In the ELISA method, a test liquid containing a test target substance is added to an object on which an antibody is immobilized, thereby causing an antigen-antibody reaction. Thereafter, a labeled antibody solution containing an enzyme-labeled antibody is added to the object on which the antibody is immobilized, thereby causing an antigen-antibody reaction. After the object on which the antibody is immobilized is washed with the washing liquid, the enzyme substrate is added to the object on which the antibody is immobilized. Optical measurement is performed by irradiating a fluorescent substance or a colored substance produced by the enzyme reaction with detection light.

  In the ELISA method, an enzyme present in the test liquid may affect the test result. For this reason, a two-step method in which washing is performed after the reaction between the immobilized antibody and the test liquid is performed, and an enzyme-labeled antibody is added after the washing may be employed.

JP 2013-50435 A

  In the case where the above two-step method is employed, if the enzyme-labeled antibody contacts the test liquid before reacting with the immobilized antibody, the enzyme-labeled antibody reacts with the test liquid, and the test accuracy decreases. there is a possibility.

  The objective of this invention is providing the test | inspection chip which reduces the possibility that test | inspection precision will fall.

The test chip according to the present invention is formed to be recessed in one direction and holds a test liquid holding test liquid containing a substance to be tested, and is formed to be recessed in the one direction and holds a first cleaning liquid. The first cleaning liquid holding part, the labeled antibody liquid holding part formed to be recessed in the one direction and holding the labeled antibody liquid containing the enzyme labeled antibody, the test liquid holding part and the first cleaning liquid holding part are first. A common receiving part connected via a guide channel and the labeled antibody solution holding part connected via a second guide channel, and a solid-phased antibody arranged downstream of the common receiving part are arranged. Forming a reaction liquid holding portion, a test liquid holding portion, a first wall surface for guiding the test liquid to the first guide channel, a first cleaning liquid holding portion, and the first cleaning liquid Forms the second wall surface for guiding to one guide channel and the labeled antibody solution holding part , And a third wall face that guides the labeled antibody solution into the second guiding passage, the inclination angle R1 is a angle between the direction and the first wall face, and the one direction and the second wall the inclination angle R2 is an angle of said at which the inclination angle R3 is the angle between the third wall and said one direction, a inspection chip that have a relationship of R3>R2> R1, the test The test chip revolves in the extending direction of each of the first wall surface, the second wall surface, and the third wall surface with respect to the direction in which the centrifugal force acts by revolving around a vertical axis separated from the chip. The first cleaning liquid held in the test liquid holding part, the first cleaning liquid held in the first cleaning liquid holding part, by switching by rotation about a horizontal axis extending in the direction of speed when , The labeled antibody solution The labeled antibody solution held in the section, characterized in that to flow into the common receiving unit in sequence.

  In this case, since the test chip satisfies R3> R2> R1, the test liquid, the first cleaning liquid, and the labeled antibody liquid can be flowed into the common receiving part in this order, and flowed into the reaction part. That is, the test liquid moves to the common receiving part and reacts with the antibody immobilized on the reaction part. The first cleaning liquid cleans the common receiving part through which the test liquid flows, and further cleans the reaction part. The labeled antibody solution moves to the common receiving part after washing and moves to the reaction part after washing. In the test chip, the test liquid holding part and the first cleaning liquid holding part are connected to the common receiving part via the first guide channel, and the labeled antibody liquid holding part is connected to the common receiving part via the second guide channel. Connected. That is, the labeled antibody holding part is provided in a different path from the test liquid holding part and the first cleaning liquid holding part with reference to the common receiving part. Therefore, compared to the case where the test liquid holding unit and the labeled sample liquid holding unit are provided in the same path with reference to the common receiving unit, the test liquid held in the test liquid holding unit and the labeled sample liquid holding unit It is difficult to come into contact with the labeled specimen solution held in For this reason, possibility that inspection accuracy will fall can be reduced. The first cleaning liquid cleans the test liquid, but the first cleaning liquid containing the test liquid may adhere to the flow path through which the first cleaning liquid has passed. However, since the labeled antibody solution flows into the common receiving part through a different path from the test liquid and the first cleaning liquid, it may come into contact with the first cleaning liquid adhering to the flow path as compared with the case where it flows through the same path. Becomes lower. Therefore, it is difficult for the test liquid and the labeled specimen liquid to come into contact with each other. For this reason, possibility that inspection accuracy will fall can be reduced.

  The test chip is formed to be recessed in the one direction, and is provided with a stop liquid holding part for holding a stop liquid for stopping the progress of the enzyme reaction, and provided downstream of the stop liquid holding part, and is recessed in the one direction. And a substrate solution holding unit that holds a substrate solution for an enzyme reaction, and the labeled antibody solution holding unit may be provided downstream of the substrate solution holding unit.

The test chip is provided downstream of the substrate solution holding unit and upstream of the labeled antibody solution holding unit, is formed to be recessed in the one direction, and holds a second cleaning solution holding unit, A first receiving part provided downstream of the stop liquid holding part and upstream of the substrate solution holding part; a second receiving part provided downstream of the substrate solution holding part and upstream of the second cleaning liquid holding part; Forming a stop receiving part and a third receiving part provided downstream of the second cleaning liquid holding part and upstream of the labeled antibody solution holding part, and guiding the stop liquid to the first receiving part. Four wall surfaces and a fifth wall surface for forming the substrate solution holding part, guiding the substrate solution to the second receiving part, and forming the second cleaning liquid holding part, and supplying the second cleaning liquid to the third receiving part and a sixth wall that guides to, I and the one direction and the first wall The inclination angle R1 is an angular, the inclination angle R2 which is the angle between the direction and the second wall surface, the inclination angle R3 is a angle between the direction and the third wall surface, the fourth wall And an inclination angle R4 that is an angle between the one direction, an inclination angle R5 that is an angle between the fifth wall surface and the one direction, and an inclination angle that is an angle between the sixth wall surface and the one direction. the R 6 have a relationship of R3, R4, R5, R6>R2> R1, relative to the direction in which acts a centrifugal force by the revolution, the fourth wall, the fifth wall, and each of the sixth wall By switching the extending direction by the rotation, when the labeled antibody solution held in the labeled antibody solution holding portion flows into the common receiving portion, the stop solution held in the stop solution holding portion is changed. Flow into the first receiving part and hold in the substrate solution holding part The substrate solution was allowed to flow into the second receiving portion, the second cleaning liquid retained in the second washing solution holding portion may be flowed into the third receiving portion.

The inspection chip includes a seventh wall surface that forms the reaction portion and guides liquid downstream in the reaction portion , an inclination angle R2 that is an angle formed by the second wall surface and the one direction, and the first a is the angle of inclination R7 angle between seven wall and said one direction, have a relation of R2 ≧ R7, relative to the direction in which the centrifugal force acts by the revolution, the direction of extension of the seventh wall, by the rotation By switching, the liquid held in the reaction section may be guided downstream before or simultaneously with the first cleaning liquid held in the first cleaning liquid holding section flowing into the common receiving section .

  In the test chip, the first cleaning liquid holding part is located in the one direction from the test liquid holding part, the labeled antibody liquid holding part is located in the one direction from the first cleaning liquid holding part, and the first The downstream first outflow end portion in the one cleaning liquid holding portion is positioned in the one direction from the first imaginary line drawn perpendicularly to the first wall surface from the downstream second outflow end portion in the inspection liquid holding portion. The third outflow end portion on the downstream side in the labeled antibody solution holding portion may be located in the one direction from the second imaginary line drawn perpendicularly from the first outflow end portion to the second wall surface. .

It is a figure which shows the structure of the test | inspection system 3 containing the test | inspection apparatus 1 and the control apparatus 90. FIG. It is a front view of the test | inspection chip 2. FIG. It is a state transition diagram of the test | inspection chip 2 in a centrifugation process. FIG. 4 is a state transition diagram continued from FIG. 3. FIG. 5 is a state transition diagram continued from FIG. 4. It is a figure which shows a mode that the test | inspection liquid 91 flows from the test | inspection liquid holding | maintenance part 310. FIG. It is a figure which shows a mode that the 1st cleaning liquid 92 flows from the 1st cleaning liquid holding | maintenance part 320. FIG. It is a figure which shows a mode that the labeled antibody liquid 93 flows from the labeled antibody liquid holding | maintenance part 330. FIG.

  DESCRIPTION OF EMBODIMENTS Embodiments embodying the present invention will be described with reference to the drawings. FIG. 1 shows a plane of the inspection apparatus 1 constituting the inspection system 3 and functional blocks inside the control apparatus 90.

<1. Schematic structure of inspection system 3>
A schematic structure of the inspection system 3 will be described with reference to FIG. The inspection system 3 of the present embodiment includes an inspection chip 2 that can store a reagent that is a liquid, and an inspection device 1 that performs inspection using the inspection chip 2. When the inspection device 1 rotates the inspection chip 2 around the vertical axis A <b> 1 separated from the inspection chip 2, centrifugal force acts on the inspection chip 2. When the inspection apparatus 1 rotates the inspection chip 2 around the horizontal axis A2, the centrifugal direction, which is the direction of the centrifugal force acting on the inspection chip 2, is switched. In addition, since the inspection system 3 and the inspection apparatus 1 of this embodiment have a known structure as described in JP 2012-78107 A, in the following description, an outline of the structure of the inspection apparatus 1 will be described. To do.

<2. Structure of the inspection apparatus 1>
The structure of the inspection apparatus 1 will be described with reference to FIG. In the following description, the upper side, the lower side, the right side, the left side, the front side of the paper surface, and the rear side of the paper surface in FIG. . In the present embodiment, the direction of the vertical axis A1 is the vertical direction of the inspection apparatus 1, and the direction of the horizontal axis A2 is the direction of the speed when the inspection chip 2 is rotated about the vertical axis A1. FIG. 1 shows a state in which the top plate of the upper housing 30 of the inspection apparatus 1 has been removed.

  As shown in FIG. 1, the inspection apparatus 1 includes an upper housing 30, a lower housing 29, an upper plate 32, a turntable 33, an angle changing mechanism 34, and a control device 90. The turntable 33 is a disk rotatably provided on the upper side of an upper plate 32 described later. The inspection chip 2 is held above the turntable 33. The angle changing mechanism 34 is a drive mechanism provided on the turntable 33. The angle changing mechanism 34 rotates the inspection chip 2 around the horizontal axis A2. The upper housing 30 is fixed to an upper plate 32 described later, and a measurement unit 7 that performs optical measurement on the inspection chip 2 is provided inside. The control device 90 is a controller that controls various processes of the inspection device 1.

  A schematic structure of the lower housing 29 will be described. The lower housing 29 has a box-like frame structure in which frame members are combined. An upper plate 32 that is a rectangular plate material is provided on the upper surface of the lower housing 29. A drive mechanism for rotating the turntable 33 around the vertical axis A1 is provided in the lower housing 29 as follows.

  A spindle motor 35 that supplies a driving force for rotating the turntable 33 is installed on the left side of the lower housing 29. A shaft 36 of the main shaft motor 35 protrudes upward, and a pulley 37 is fixed. A vertical main shaft 57 extending upward from the inside of the lower housing 29 is provided at the center of the lower housing 29. The main shaft 57 passes through the upper plate 32 and protrudes above the lower housing 29. The upper end portion of the main shaft 57 is connected to the center portion of the turntable 33.

  The main shaft 57 is rotatably held by a support member (not shown) provided immediately below the upper plate 32. A pulley 38 is fixed to the main shaft 57 below the support member. A belt 39 is stretched over the pulley 37 and the pulley 38. When the main shaft motor 35 rotates the shaft 36, the driving force is transmitted to the main shaft 57 via the pulley 37, the belt 39, and the pulley 38. At this time, the turntable 33 rotates around the main shaft 57 in conjunction with the rotation of the main shaft 57.

  A guide rail (not shown) extending in the vertical direction inside the lower housing 29 is provided on the right side in the lower housing 29. A T-shaped plate (not shown) is movable in the vertical direction in the lower housing 29 along the guide rail.

  The aforementioned main shaft 57 is a cylindrical body having a hollow inside. An inner shaft (not shown) is a shaft that can move in the vertical direction inside the main shaft 57. The upper end portion of the inner shaft passes through the main shaft 57 and is connected to the rack gear 43. A bearing (not shown) is provided at the left end of the T-shaped plate. Inside the bearing, the lower end portion of the inner shaft is rotatably held.

  A stepping motor 51 for moving the T-shaped plate up and down is fixed in front of the T-shaped plate. The shaft 58 of the stepping motor 51 protrudes rearward, that is, downward in FIG. A disc-shaped cam plate (not shown) is fixed to the tip of the shaft 58. A cylindrical projection (not shown) is provided on the rear surface of the cam plate. The tip of the protrusion is inserted into a groove (not shown). The protrusion can slide in the groove. When the stepping motor 51 rotates the shaft 58, the protrusion moves up and down in conjunction with the rotation of the cam plate. At this time, the T-shaped plate moves up and down along the guide rail in conjunction with the protrusion inserted in the groove.

  The detailed structure of the angle changing mechanism 34 will be described. The angle changing mechanism 34 has a pair of L-shaped plates 60 fixed to the upper surface of the turntable 33. Each L-shaped plate 60 extends upward from a base portion fixed in the vicinity of the center of the turntable 33, and its upper end portion extends outward in the radial direction of the turntable 33. A rack gear 43 (not shown) fixed to the inner shaft is provided between the pair of L-shaped plates 60. The rack gear 43 is a metal plate-like member that is long in the vertical direction, and gears are respectively carved on both end faces.

  On the front end side in the extending direction of each L-shaped plate 60, a horizontal support shaft 46 having a gear 45 is rotatably supported. The support shaft 46 is fixed to the inspection chip 2 via a mounting holder (not shown). For this reason, the inspection chip 2 also rotates around the support shaft 46 in conjunction with the rotation of the gear 45. Between the gear 45 and the rack gear 43, a pinion gear 44 supported by an L-shaped plate 60 so as to be rotatable about a horizontal axis (not shown) is interposed. The pinion gear 44 meshes with the gear 45 and the rack gear 43, respectively. In conjunction with the vertical movement of the rack gear 43, the pinion gear 44 and the gear 45 are driven to rotate, and the inspection chip 2 is rotated about the support shaft 46.

  In the present embodiment, as the main shaft motor 35 rotationally drives the turntable 33, the inspection chip 2 rotates around the main shaft 57 that is a vertical axis, and a centrifugal force acts on the inspection chip 2. The rotation around the vertical axis A1 of the inspection chip 2 is referred to as revolution. On the other hand, as the stepping motor 51 moves the inner shaft up and down, the inspection chip 2 rotates about the support shaft 46 which is a horizontal axis, and the direction of the centrifugal force acting on the inspection chip 2 changes relatively. The rotation around the horizontal axis A2 of the inspection chip 2 is called autorotation.

  In a state where the T-shaped plate is lowered to the lowermost end of the movable range, the rack gear 43 is also lowered to the lowermost end of the movable range. At this time, the inspection chip 2 is in a steady state where the rotation angle is 0 degree. Further, in the state where the T-shaped plate is raised to the uppermost end of the movable range, the rack gear 43 is also raised to the uppermost end of the movable range. At this time, the test | inspection chip 2 will be in the state rotated 180 degree | times centering on the horizontal axis line A2 from the steady state. That is, in this embodiment, the angle width that the test chip 2 can rotate is the rotation angle of 0 degrees to 180 degrees.

  The detailed structure of the upper housing 30 will be described. As shown in FIG. 1, the upper housing 30 has a box-like frame structure in which frame members are combined, and is installed on the upper left side of the upper plate 32. More specifically, the upper housing 30 is provided outside the range in which the inspection chip 2 is rotated as viewed from the main shaft 57 at the rotation center of the turntable 33.

  The measurement unit 7 provided inside the upper housing 30 includes a light source 71 that emits measurement light, and an optical sensor 72 that detects the measurement light emitted from the light source 71. The light source 71 and the optical sensor 72 are disposed on both the front and rear sides of the turntable 33 outside the rotation range of the inspection chip 2. In the present embodiment, the position on the left side of the main shaft 57 in the reciprocable range of the inspection chip 2 is the measurement position at which the inspection chip 2 is irradiated with the measurement light. When the inspection chip 2 is at the measurement position, the measurement light connecting the light source 71 and the optical sensor 72 intersects the front surface and the rear surface of the inspection chip 2 substantially perpendicularly.

<3. Electrical configuration of control device 90>
The electrical configuration of the control device 90 will be described with reference to FIG. The control device 90 includes a CPU 101 that controls the main control of the inspection device 1, a RAM 102 that temporarily stores various data, and a ROM 103 that stores a control program. Connected to the CPU 101 are an operation unit 104 for a user to input an instruction to the control device 90, a hard disk device 105 for storing various data and programs, and a display 106 for displaying various information. As the control device 90, a personal computer may be used, or a dedicated control device may be used.

  Further, a revolution controller 97, a rotation controller 98, and a measurement controller 99 are connected to the CPU 101. The revolution controller 97 controls the revolution of the inspection chip 2 by transmitting a control signal for rotating the spindle motor 35 to the spindle motor 35. The rotation controller 98 controls the rotation of the inspection chip 2 by transmitting a control signal for rotating the stepping motor 51 to the stepping motor 51. The measurement controller 99 performs the optical measurement of the inspection chip 2 by driving the measurement unit 7. Specifically, the measurement controller 99 transmits a control signal for executing light emission of the light source 71 and light detection of the optical sensor 72 to the light source 71 and the optical sensor 72. The CPU 101 controls the revolution controller 97, the rotation controller 98, and the measurement controller 99.

<4. Structure of inspection chip 2>
With reference to FIG. 2, the detailed structure of the test | inspection chip 2 which concerns on this embodiment is demonstrated. In the following description, the upper, lower, left, right, front side, and back side of FIG. 2 are the upper, lower, left, right, front, and rear sides of the inspection chip 2, respectively. . In the present embodiment, the inspection chip 2 is used, and the inspection is performed by the ELISA method.

  As shown in FIG. 2, the inspection chip 2 has a square shape having an upper side portion 81, a lower side portion 84, a right side portion 82, and a left side portion 83 when viewed from the front as an example, and is a transparent composite having a predetermined thickness. Resin plate 19 is mainly used. On the upper right portion of the inspection chip 2, a protruding portion 205 protruding upward is provided. The protrusion 205 is gripped by the user, for example. At the upper left part of the inspection chip 2, a protruding part 206 protruding leftward is provided.

  The front surface 203 of the plate 19 is sealed with a sheet 291 made of a transparent synthetic resin thin plate. Between the plate material 19 and the sheet 291, a liquid flow path 25 is formed through which the liquid sealed in the inspection chip 2 can flow. The liquid channel 25 is a recess formed at a predetermined depth on the front surface 203 of the plate material 19 and extends in a direction orthogonal to the front-rear direction, which is the thickness direction of the plate material 19. The sheet 291 seals the flow path forming surface of the plate material 19. The sheet 291 is not shown in FIGS.

  The liquid flow path 25 includes a test liquid holding part 310, a first cleaning liquid holding part 320, a labeled antibody liquid holding part 330, a second cleaning liquid holding part 340, a substrate solution holding part 350, a stop liquid holding part 360, a common receiving part 370, Reaction unit 380, measurement unit 390, waste liquid unit 700, first receiving unit 450, second receiving unit 460, third receiving unit 470, fourth receiving unit 550, injection path 480, 490.500, 510, 520, 530, etc. including. The test liquid holding part 310, the first cleaning liquid holding part 320, the labeled antibody liquid holding part 330, the second cleaning liquid holding part 340, the substrate solution holding part 350, the stop liquid holding part 360, the reaction part 380, and the measuring part 390 are respectively provided. It is recessed downward and opened upward. In the following description, the test liquid holding unit 310, the first cleaning liquid holding unit 320, the labeled antibody liquid holding unit 330, the second cleaning liquid holding unit 340, the substrate solution holding unit 350, the stop liquid holding unit 360, the reaction unit 380, and The downward direction, which is the direction in which the measurement unit 390 is recessed, may be referred to as the formation direction.

  The immobilized antibody is disposed on the wall surface forming the reaction part 380. The test liquid holding unit 310, the first cleaning liquid holding unit 320, the labeled antibody liquid holding unit 330, the second cleaning liquid holding unit 340, the substrate solution holding unit 350, and the stop liquid holding unit 360 are the test liquid 91 and the first cleaning liquid, respectively. 92, a site where the labeled antibody solution 93, the second washing solution 94, the substrate solution 95, and the stop solution 96 are held.

  The test liquid 91 is a liquid containing components of a test target substance such as blood, plasma, blood cells, bone marrow, urine, vaginal tissue, epithelial tissue, tumor, semen, saliva, animal tissue, animal blood, or foodstuffs. It is. When the test liquid 91 flows into the reaction unit 380, the test target substance contained in the test liquid 91 is bound to the solid-phased antibody. The first cleaning liquid 92 removes components of the test liquid 91 that are not bound to the antibody in the reaction unit 380. The labeled antibody solution 93 includes an enzyme labeled antibody. An enzyme-labeled antibody is an antibody labeled with an enzyme. When the labeled antibody solution 93 flows into the reaction unit 380, the enzyme-labeled antibody specifically binds to the conjugate of the test target substance and the antibody.

  In the reaction part 380, the second washing liquid 94 removes the components of the labeled antibody liquid 93 that are not bound to the conjugate of the substance to be examined and the antibody. The substrate solution 95 is a liquid that comes into contact with the antibody after being washed with the second washing liquid 94 and reacts with the enzyme-labeled antibody. The stop liquid 96 is a liquid mixed with the substrate solution 95 and is a liquid that stops the progress of the enzyme reaction. In the present embodiment, in the optical measurement described later, a fluorescent substance or a colored substance generated by an enzyme reaction of the substrate solution 95 can be detected to measure the inspection target substance. In the following description, the first washing solution 92, the labeled antibody solution 93, the second washing solution 94, the substrate solution 95, and the stop solution 96 are collectively referred to as “reagent 9” or when none of them is specified.

  The inspection liquid holding unit 310 is provided in the upper right part of the inspection chip 2. The injection path 480 extends upward from the upper left part of the test liquid holding part 310 and penetrates the upper side part 81. The injection path 480 is a part for injecting the test liquid 91 into the test liquid holding unit 310. The first cleaning liquid holding unit 320 is located in the formation direction from the test liquid holding unit 310. The injection path 490 extends upward from the upper left part of the first cleaning liquid holding part 320 and penetrates the upper side part 81. The injection path 490 is a part for injecting the first cleaning liquid 92 into the first cleaning liquid holding unit 320. The labeled antibody solution holding unit 330 is provided in the lower left part of the test chip 2. That is, the labeled antibody solution holding unit 330 is positioned in the forming direction from the first cleaning solution holding unit 320. The injection path 500 extends upward from the upper left part of the labeled antibody solution holding part 330 and penetrates the upper side part 81. The injection path 500 is a part for injecting the labeled antibody solution 93 into the labeled antibody solution holding unit 330.

  A common receiving portion 370 is provided on the lower right side of the first cleaning liquid holding portion 320. The common receiving portion 370 is a concave portion that opens upward. The common receiving part 370 is connected to the test liquid holding part 310 and the first cleaning liquid holding part 320 via the first guide channel 610. The first guide channel 610 is a channel extending downward from the upper right part of the test liquid holding part 310 and the upper right part of the first cleaning liquid holding part 320. The first guide channel 610 is located in a region on the right side of the first reference line 611 indicated by a two-dot chain line in FIG.

  The stop liquid holding unit 360 is provided in the upper left part of the inspection chip 2. The injection path 530 extends upward from the upper left part of the stop liquid holding part 360 and penetrates the upper side part 81. The injection path 530 is a part for injecting the stop liquid 96 into the stop liquid holding unit 360. The substrate solution holding part 350 is located in the formation direction from the stop liquid holding part 360. The injection path 520 extends upward from the upper left part of the substrate solution holding part 350 and penetrates the upper side part 81. The injection path 520 is a part for injecting the substrate solution 95 into the substrate solution holding unit 350.

  The second cleaning liquid holding unit 340 is located in the formation direction from the substrate solution holding unit 350. The injection path 510 extends upward from the upper left part of the second cleaning liquid holding part 340 and penetrates the upper side part 81. The injection path 510 is a part for injecting the second cleaning liquid 94 into the second cleaning liquid holding unit 340. The labeled antibody solution holding unit 330 is positioned in the forming direction from the substrate solution holding unit 350. The injection path 500 extends upward from the upper left part of the labeled antibody solution holding part 330 and penetrates the upper side part 81. The injection path 500 is a part for injecting the labeled antibody solution 93 into the labeled antibody solution holding unit 330.

  The first receiving part 450 is a flow path extending downward from the upper right part of the stop liquid holding part 360. The first receiving portion 450 is recessed to the right. The lower left portion of the first receiving portion 450 is connected to the substrate solution holding portion 350. That is, the substrate solution holding part 350 is provided downstream of the stop liquid holding part 360. Further, the first receiving part 450 is provided downstream of the stop liquid holding part 360 and upstream of the substrate solution holding part 350.

  The second receiving part 460 is a flow path that extends downward from the upper right part of the substrate solution holding part 350. The second receiving part 460 is recessed rightward. The lower left part of the second receiving part 460 is connected to the second cleaning liquid holding part 340. That is, the second receiving part 460 is provided downstream of the substrate solution holding part 350 and upstream of the second cleaning liquid holding part 340.

  The third receiving part 470 is a flow path extending downward from the upper right part of the second cleaning liquid holding part 340. The third receiving part 470 is recessed to the right. The lower left part of the third receiving part 470 is connected to the labeled antibody solution holding part 330. That is, the labeled antibody solution holding unit 330 is provided downstream of the substrate solution holding unit 350. The second cleaning liquid holding unit 340 is provided downstream of the substrate solution holding unit 350 and upstream of the labeled antibody liquid holding unit 330. The third receiving part 470 is provided downstream of the second cleaning liquid holding part 340 and upstream of the labeled antibody liquid holding part 330.

  The right part of the labeled antibody solution holding unit 330 extends obliquely upward to the right, and the upper end thereof extends rightward below the first cleaning solution holding unit 320. The common receiving part 370 is connected to the upper right part of the labeled antibody solution holding part 330 via the second guide channel 620. The second guide channel 620 is a channel that extends from the upper right part of the labeled antibody solution holding unit 330 in a direction perpendicular to a third wall surface 503 described later. The second guide channel 620 is located in a region below the second reference line 621 and above the second reference line 622 indicated by a two-dot chain line in FIG.

  The lower left part of the common receiving part 370 is connected to the reaction part 380. The fourth receiving part 550 is a flow path that extends downward from the upper right part of the reaction part 380. The fourth receiving portion 550 is recessed rightward. The lower left part of the fourth receiving part 550 is connected to the measuring part 390. The upper right part of the measurement part 390 is connected to the waste liquid part 700. The waste liquid part 700 includes a first waste liquid part 710, a second waste liquid part 720, a third waste liquid part 730, and a waste liquid holding part 740. The reagent 9 that flows out from the measuring section 390 to the waste liquid section 700 is referred to as a waste liquid 85 shown in FIG. At least a part of the waste liquid 85 that has flowed into the waste liquid part 700 is held in the waste liquid holding part 740, but detailed description thereof is omitted.

  A wall surface downstream of the wall surfaces forming the test liquid holding unit 310 and guiding the test liquid 91 to the first guide channel 610 is referred to as a first wall surface 501. A wall surface downstream of the wall surfaces forming the first cleaning liquid holding unit 320 and guiding the first cleaning liquid 92 to the first guide channel 610 is referred to as a second wall surface 502. A wall surface downstream of the wall surfaces forming the labeled antibody solution holding unit 330 and guiding the labeled antibody solution 93 to the second guide channel 620 is referred to as a third wall surface 503. A wall surface downstream of the wall surfaces forming the stop liquid holding portion 360 and guiding the stop liquid 96 to the first receiving portion 450 is referred to as a fourth wall surface 504. A wall surface downstream of the wall surfaces forming the substrate solution holding unit 350 and guiding the substrate solution 95 to the second receiving unit 460 is referred to as a fifth wall surface 505. A wall surface downstream of the wall surfaces forming the second cleaning liquid holding unit 340 and guiding the second cleaning liquid 94 to the third receiving unit 470 is referred to as a sixth wall surface 506. A wall surface downstream of the wall surfaces forming the reaction unit 380 and guiding the reagent 9 to the fourth receiving unit 550 is referred to as a seventh wall surface 507.

The inclination angles of the first to seventh wall surfaces 501 to 507 are R1, R2, R3, R4, R5, R6, and R7, respectively. In FIG. 2, as an example, the inclination angles R <b> 1 to R <b> 7 are indicated by angles formed by the first to seventh wall surfaces 501 to 507 and the forming direction. The inclination angles R1 to R6 have the relationship of the following formula (1). Further, the inclination angles R2 and R7 have the relationship of the following formula (2).
R3, R4, R5, R6>R2> R1 (1)
R2 ≧ R7 (2)

  In FIG. 2, R3 to R6 have the same angle as each other, but R3 to R6 may have different angles as long as R3 to R6 are larger than R2. In FIG. 2, R2 and R7 have the same angle, but R2 and R7 may have different angles as long as R2 is equal to or greater than R7.

  In addition, an imaginary line drawn perpendicularly to the first wall surface 501 from the downstream second outflow end 522 in the test liquid holding unit 310 is referred to as a first imaginary line 511. A virtual line drawn perpendicularly to the second wall surface 502 from the downstream first outflow end 521 in the first cleaning liquid holding unit 320 is referred to as a second virtual line 512. The first outflow end 521 is located in the formation direction from the first virtual line 511. The downstream third outflow end portion 523 in the labeled antibody solution holding unit 330 is located in the formation direction from the second virtual line 512.

  In the liquid flow path 25 of the test chip 2 of the present embodiment, liquids such as the test liquid 91 and other reagents 9 are supplied from the test liquid holding unit 310 and the first cleaning liquid holding unit 320 through the common receiving unit 370. It flows to the reaction unit 380. Thus, for example, the reaction unit 380 is provided downstream of the common receiving unit 370. Similarly, for example, the stop solution 96 flows from the stop solution holding unit 360 to the common receiving unit 370 via the substrate solution holding unit 350, the second washing solution holding unit 340, and the labeled antibody solution holding unit 330.

<5. Other structures of inspection chip 2>
As shown in FIG. 1, the support shaft 46 extending from the L-shaped plate 60 is vertically connected to the center of the rear surface of the plate member 19 via a mounting holder (not shown). As the support shaft 46 rotates, the inspection chip 2 rotates around the support shaft 46. When the inspection chip 2 is in the steady state shown in FIG. 2, the upper side portion 81 and the lower side portion 84 are orthogonal to the direction of gravity G, the right side portion 82 and the left side portion 83 are parallel to the direction of gravity G, and the left side portion 83. Is disposed closer to the main shaft 57 than the right side portion 82. In a state where the inspection chip 2 in the steady state is arranged at the measurement position, the inspection apparatus 1 performs inspection by optical measurement by allowing the measurement light connecting the light source 71 and the optical sensor 72 to pass through the measurement unit 390.

<6. Example of inspection method>
An inspection method using the inspection apparatus 1 and the inspection chip 2 will be described. As shown in FIG. 2, the stop liquid 96 is injected into the stop liquid holding unit 360 via the injection path 530. The substrate solution 95 is injected into the substrate solution holding unit 350 via the injection path 520. The second cleaning liquid 94 is injected into the second cleaning liquid holding unit 340 via the injection path 510. The labeled antibody solution 93 is injected into the labeled antibody solution holding unit 330 through the injection path 500. The first cleaning liquid 92 is injected into the first cleaning liquid holding unit 320 via the injection path 490. The inspection liquid 91 is injected into the inspection liquid holding unit 310 via the injection path 480.

  The arrangement method of the reagent 9 is not limited. For example, a hole may be opened at a position corresponding to the holding portions 310, 320, 330, 340, 350, and 360 in the sheet 291, and the user may inject the reagent 9 from the hole and then seal and seal. . Alternatively, the reagent 9 may be arranged in advance in the holding portions 320, 330, 340, 350, and 360 and sealed with the sheet 291. In this case, a hole may be opened at a position corresponding to the test liquid holding unit 310 in the sheet 291, and the user may inject the test liquid 91 from the hole and then seal and seal the sheet.

  The user attaches the inspection chip 2 to a mounting holder (not shown) and inputs a process start command from the operation unit 104 shown in FIG. As a result, the CPU 101 executes centrifugal processing based on the control program stored in the ROM 103. The inspection apparatus 1 can inspect two inspection chips 2 at the same time. For convenience of explanation, a procedure for inspecting one inspection chip 2 will be described below. Moreover, the steady state of the test | inspection chip 2 shown in FIG. 2 is called rotation angle 0 degree, and the state rotated 90 degree | times counterclockwise from the steady state is called rotation angle 90 degree | times. Moreover, the rotation angle of the test | inspection chip 2 in case the centrifugal force X acts on the direction perpendicular | vertical to the 1st wall surface 501 shown to FIG. 3 (A) is called 1st rotation angle. The rotation angle of the test chip 2 when the centrifugal force X is applied in a direction perpendicular to the second wall surface 502 shown in FIG. 3C is referred to as a second rotation angle. The rotation angle of the test chip 2 when the centrifugal force X is applied in the direction perpendicular to the seventh wall surface 507 shown in FIG. 5 (M) is referred to as a third rotation angle.

  When the CPU 101 rotates the inspection chip 2 from the rotation angle 0 degree, the first rotation angle, the second rotation angle, or the third rotation angle to 90 degrees, and when rotating the inspection chip 2 from the rotation angle 0 degrees to the first rotation angle, The inspection chip 2 rotates counterclockwise when viewed from the front. Further, when the CPU 101 rotates the inspection chip 2 from the rotation angle of 90 degrees to 0 degree, the second rotation angle, or the third rotation angle, the inspection chip 2 rotates clockwise as viewed from the front.

  The CPU 101 reads motor driving information stored in advance in the HDD 105, sets driving information of the spindle motor 35 in the revolution controller 97, and sets driving information of the stepping motor 51 in the rotation controller 98. At this time, as shown in FIG. 2, the inspection chip 2 is in a steady state and has a rotation angle of 0 degree. Next, the CPU 101 shown in FIG. 1 controls the revolution controller 97 to start driving the spindle motor 35. As a result, the revolution of the inspection chip 2 is started. Further, the CPU 101 controls the rotation controller 98 to drive the stepping motor 51. And CPU101 rotates the test | inspection chip 2 to the 1st autorotation angle shown to FIG. 3 (A). The spindle motor 35 increases the rotation speed of the turntable 33 to the speed V based on an instruction from the revolution controller 97. The speed V is, for example, 3000 rpm. When the turntable 33 is rotated at this speed V, a centrifugal force X of several hundred G acts on the inspection chip 2. In the following description, the rotation speed of the turntable 33 is assumed to be constant at the speed V, but the value of the speed V may be changed during the centrifugal process.

  The CPU 101 maintains the rotation speed of the spindle motor 35 at the speed V. As shown in FIG. 3A, centrifugal force X acts on the test chip 2 in a direction perpendicular to the first wall surface 501. Due to the action of the centrifugal force X, the test liquid 91 moves from the test liquid holding unit 310 to the common receiving unit 370. At this time, as shown in FIG. 6, the test liquid 91 moves to the first guide channel 610 along the first virtual line 511 and moves to the common receiving portion 370 along the first guide channel 610. In addition, since it has the relationship of the said Formula (1), as shown to FIG. 3 (A), the 1st washing | cleaning liquid 92, the labeled antibody liquid 93, the 2nd washing | cleaning liquid 94, the substrate solution 95, and the stop liquid 96 are respectively respectively. The first cleaning liquid holding part 320, the labeled antibody liquid holding part 330, the second cleaning liquid holding part 340, the substrate solution holding part 350, and the stop liquid holding part 360 are held.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 3B, the CPU 101 rotates the inspection chip 2 up to a rotation angle of 90 degrees. A centrifugal force X acts on the inspection chip 2 from the upper side portion 81 toward the lower side portion 84. Due to the action of the centrifugal force X, the test liquid 91 moves from the common receiving part 370 to the reaction part 380. When the test liquid 91 moves to the reaction unit 380, the test target substance contained in the test liquid 91 is bound to the immobilized antibody.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 3C, the CPU 101 rotates the inspection chip 2 to the second rotation angle. Centrifugal force X acts on the inspection chip 2 in a direction perpendicular to the second wall surface 502. Due to the action of the centrifugal force X, the first cleaning liquid 92 moves from the first cleaning liquid holding part 320 to the common receiving part 370. At this time, as shown in FIG. 7, the first cleaning liquid 92 moves to the first guide channel 610 along the second imaginary line 512 and moves to the common receiving portion 370 along the first guide channel 610. . In addition, the test liquid 91 moves from the reaction unit 380 to the fourth receiving unit 550. In addition, since the said Formula (2) is satisfy | filled, before the 1st washing | cleaning liquid 92 flows out toward the common receiving part 370 from the 1st washing | cleaning liquid holding | maintenance part 320, or simultaneously, the test | inspection liquid 91 is the 4th receiving part 550 from the reaction part 380. It flows toward. Further, since the above formula (1) is satisfied, as shown in FIG. 3C, the labeled antibody solution 93, the second washing solution 94, the substrate solution 95, and the stop solution 96 are respectively labeled antibody solution holding unit 330, The second cleaning liquid holding unit 340, the substrate solution holding unit 350, and the stop liquid holding unit 360 are held.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 3D, the CPU 101 rotates the inspection chip 2 up to a rotation angle of 90 degrees. A centrifugal force X acts on the inspection chip 2 from the upper side portion 81 toward the lower side portion 84. Due to the action of the centrifugal force X, the first cleaning liquid 92 moves from the common receiving part 370 to the reaction part 380. The first cleaning liquid 92 removes components of the test liquid 91 that are not bound to the antibody in the reaction unit 380. Further, the test liquid 91 moves from the fourth receiving part 550 to the measuring part 390.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 3E, the CPU 101 rotates the inspection chip 2 up to a rotation angle of 0 degree. A centrifugal force X acts on the inspection chip 2 from the left side portion 83 toward the right side portion 82. Due to the action of the centrifugal force X, the first cleaning liquid 92, the second cleaning liquid 94, the substrate solution 95, and the stop liquid 96 are supplied to the fourth receiving portion 550, the third receiving portion 470, the second receiving portion 460, and the first receiving portion 460, respectively. It moves to the receiving part 450.

  Further, the labeled antibody solution 93 moves from the labeled antibody solution holding unit 330 to the right part of the common receiving unit 370 and the first guide channel 610. More specifically, the test chip 2 is perpendicular to the third wall surface 503 shown in FIG. 8 in the process of changing from the rotation angle 0 degree shown in FIG. 3D to the rotation angle 90 degree shown in FIG. Centrifugal force X acts in the direction. As shown in FIG. 8, the labeled antibody solution 93 flows into the common receiving portion 370 through the second guide channel 620. In the following description, when the reagent 9 moves from the labeled antibody solution holding unit 330 to the right part of the common receiving unit 370 and the first guide channel 610, it moves from the labeled antibody solution holding unit 330 to the common receiving unit 370. That's it. Further, the inspection liquid 91 moves from the measurement unit 390 to the waste liquid unit 700 as the waste liquid 85.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 3F, the CPU 101 rotates the inspection chip 2 up to a rotation angle of 90 degrees. A centrifugal force X acts on the inspection chip 2 from the upper side portion 81 toward the lower side portion 84. By the action of the centrifugal force X, the first washing solution 92, the labeled antibody solution 93, the second washing solution 94, the substrate solution 95, and the stop solution 96 are respectively measured by the measuring unit 390, the reaction unit 380, the labeled antibody solution holding unit 330, It moves to the second cleaning liquid holding unit 340 and the substrate solution holding unit 350. Since the labeled antibody solution 93 moves to the reaction unit 380, the enzyme-labeled antibody specifically binds to the conjugate of the test target substance and the antibody.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 4G, the CPU 101 rotates the inspection chip 2 to a rotation angle of 0 degree. A centrifugal force X acts on the inspection chip 2 from the left side portion 83 toward the right side portion 82. Due to the action of the centrifugal force X, the labeled antibody solution 93, the second washing solution 94, the substrate solution 95, and the stop solution 96 become the fourth receiving portion 550, the common receiving portion 370, the third receiving portion 470, and the second receiving portion, respectively. Move to section 460. Further, the first cleaning liquid 92, that is, the new waste liquid 85 moves from the measurement unit 390 to the waste liquid part 700.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 4H, the CPU 101 rotates the inspection chip 2 up to a rotation angle of 90 degrees. A centrifugal force X acts on the inspection chip 2 from the upper side portion 81 toward the lower side portion 84. By the action of the centrifugal force X, the labeled antibody solution 93, the second washing solution 94, the substrate solution 95, and the stop solution 96 are respectively measured, 390, reaction unit 380, labeled antibody solution holding unit 330, and second washing solution holding unit. Move to 340. The reaction unit 380 is cleaned by the second cleaning liquid 94.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 4I, the CPU 101 rotates the inspection chip 2 up to a rotation angle of 0 degree. A centrifugal force X acts on the inspection chip 2 from the left side portion 83 toward the right side portion 82. Due to the action of the centrifugal force X, the second washing liquid 94, the substrate solution 95, and the stop liquid 96 are transferred from the reaction unit 380, the labeled antibody solution holding unit 330, and the second washing solution holding unit 340, respectively, to the fourth receiving unit 550, It moves to the common receiving part 370 and the third receiving part 470. The labeled antibody solution 93, that is, a new waste solution 85 moves from the measurement unit 390 to the first waste solution unit 710.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 4J, the CPU 101 rotates the inspection chip 2 up to a rotation angle of 90 degrees. A centrifugal force X acts on the inspection chip 2 from the upper side portion 81 toward the lower side portion 84. By the action of the centrifugal force X, the second washing liquid 94, the substrate solution 95, and the stop liquid 96 move to the measurement unit 390, the reaction unit 380, and the labeled antibody solution holding unit 330, respectively. The substrate solution 95 undergoes an enzyme reaction with the enzyme-labeled antibody in the reaction unit 380 washed with the second washing liquid 94.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 4 (K), the CPU 101 rotates the inspection chip 2 to a rotation angle of 0 degree. A centrifugal force X acts on the inspection chip 2 from the left side portion 83 toward the right side portion 82. By the action of the centrifugal force X, the substrate solution 95 and the stop solution 96 move from the reaction unit 380 and the labeled antibody solution holding unit 330 to the fourth receiving unit 550 and the common receiving unit 370, respectively. Further, the second cleaning liquid 94, that is, the new waste liquid 85 moves from the measurement unit 390 to the first waste liquid part 710.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 4L, the CPU 101 rotates the inspection chip 2 up to a rotation angle of 90 degrees. A centrifugal force X acts on the inspection chip 2 from the upper side portion 81 toward the lower side portion 84. By the action of the centrifugal force X, the substrate solution 95 and the stop solution 96 move to the measurement unit 390 and the reaction unit 380, respectively.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 5M, the CPU 101 rotates the inspection chip 2 to the third rotation angle. Centrifugal force X acts on the inspection chip 2 in a direction perpendicular to the seventh wall surface 507. Due to the action of the centrifugal force X, the stop liquid 96 moves from the reaction unit 380 to the fourth receiving unit 550. The substrate solution 95 is held in the measurement unit 390.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. As shown in FIG. 5N, the CPU 101 rotates the inspection chip 2 up to a rotation angle of 90 degrees. A centrifugal force X acts on the inspection chip 2 from the upper side portion 81 toward the lower side portion 84. By the action of the centrifugal force X, the stop liquid 96 moves to the measurement unit 390. In the measurement unit 390, the stop solution 96 is mixed with the substrate solution 95, and the progress of the enzyme reaction of the substrate solution 95 is stopped. In the following description, the substrate solution 95 mixed with the stop solution 96 is referred to as a measurement solution 921.

  The CPU 101 controls the rotation controller 98 to drive the stepping motor 51. The CPU 101 rotates the inspection chip 2 until the rotation angle is 0 degree. Further, the CPU 101 controls the revolution controller 97 to stop the rotation of the spindle motor 35. Therefore, the revolution of the inspection chip 2 is completed. Centrifugation is terminated. As shown in FIG. 5 (O), gravity G acts on the test chip 2 from the upper side portion 81 toward the lower side portion 84.

  After executing the centrifugal process, the CPU 101 controls the revolution controller 97 to rotate and move the inspection chip 2 to the angle of the measurement position. The measurement unit 390 holds a measurement solution 921 in which the test liquid 91 is used. The measurement controller 99 shown in FIG. 1 causes the light source 71 to emit light and transmit the measurement light to the measurement solution 921 held in the measurement unit 390. The CPU 101 performs optical measurement of the measurement solution 921 held in the measurement unit 390 based on the change amount of the measurement light received by the optical sensor 72, and acquires measurement data. The CPU 101 calculates the measurement result of the measurement solution 921 generated using the test liquid 91. The CPU 101 displays the inspection result on the display 106 shown in FIG. Note that the measurement method of the measurement solution 921 is not limited to optical measurement, and other methods may be used.

<7. Main actions and effects of this embodiment>
As described above, the measurement in the present embodiment is performed. In the present embodiment, as shown in the above formula (1), the inspection chip 2 satisfies R3>R2> R1. Therefore, as shown in FIGS. 3A to 3F, the test liquid 91, the first cleaning liquid 92, and the labeled antibody liquid 93 are caused to flow into the common receiving portion 370 in this order, and then flowed into the reaction portion 380. Can do. That is, as shown in FIGS. 3A and 3B, the test liquid 91 moves to the common receiving part 370 and reacts with the antibody immobilized on the reaction part 380. As shown in FIGS. 3C and 3D, the first cleaning liquid 92 cleans the common receiving part 370 in which the test liquid 91 flows, and further cleans the reaction part 380. As shown in FIGS. 3E and 3F, the labeled antibody solution 93 moves to the common receiving part 370 after washing and moves to the reaction part 380 after washing. In the test chip 2, the test liquid holding part 310 and the first cleaning liquid holding part 320 are connected to the common receiving part 370 via the first guide channel 610, and the labeled antibody liquid holding part 330 is connected to the second guide flow. It is connected to the common receiving part 370 through the path 620. That is, the labeled antibody liquid holding unit 330 is provided in a different path from the test liquid holding unit 310 and the first cleaning liquid holding unit 320 with the common receiving unit 370 as a reference. Therefore, compared to the case where the test liquid holding unit 310 and the labeled antibody liquid holding unit 330 are provided in the same path with reference to the common receiving unit 370, the test liquid 91 held in the test liquid holding unit 310, The labeled antibody solution 93 held in the labeled antibody solution holding unit 330 is difficult to contact. For this reason, possibility that inspection accuracy will fall can be reduced.

  The first cleaning liquid 92 cleans the test liquid 91, but the first cleaning liquid 92 containing the test liquid 91 may adhere to the flow path through which the first cleaning liquid 92 has passed. However, since the labeled antibody solution 93 flows into the common receiving portion 370 through a path shown in FIG. 8 different from the path through which the test liquid 91 and the first cleaning liquid 92 shown in FIGS. Compared to the case of inflow, the possibility of contact with the first cleaning liquid 92 attached to the flow path is reduced. Therefore, it is difficult for the test liquid 91 and the labeled antibody solution 93 to come into contact with each other. For this reason, possibility that inspection accuracy will fall can be reduced.

  Further, as shown in the above formula (1), since the test chip 2 satisfies R3> R2> R1, each of the test liquid holding unit 310, the first cleaning liquid holding unit 320, and the labeled antibody liquid holding unit 330 is sequentially arranged. The reagent 9 held in the holding units 310, 320, and 330 is difficult to flow downstream. Since the labeled antibody solution holding unit 330 is provided downstream of the stop solution holding unit 360 and the substrate solution holding unit 350, as shown in FIGS. 4 (H) and 4 (J), the substrate solution 95 and The stop solution 96 moves to the labeled antibody solution holding unit 330 where the reagent 9 hardly flows downstream. For this reason, compared with the case where the stop liquid holding part 360 and the substrate solution holding part 350 are provided upstream of the first cleaning liquid holding part 320 or the test liquid holding part 310, the substrate solution 95 and the stop liquid 96 Is unlikely to flow downstream. Therefore, it is possible to reduce the possibility that the substrate solution 95 and the stop solution 96 flow out downstream and mix with other reagents 9. Therefore, the possibility that the inspection accuracy is lowered can be reduced.

  Further, suppose that the first receiving portion 450, the second receiving portion 460, and the third receiving portion 470 shown in FIG. 2 are not provided. In this case, R4> R5> R6> R3> R2 in order to prevent the reagent 9 held in each holding unit 310, 320, 330, 340, 350, 360 from being mixed with the liquid held in the downstream holding unit. It is necessary to make> R1. Therefore, the difference between the inclination angles R1 to R6 of the first to sixth wall surfaces 501 to 506 becomes small, and the reagent 9 may flow out downstream at an unexpected timing. In the present embodiment, since the inspection chip 2 includes the first receiving portion 450, the second receiving portion 460, and the third receiving portion 470, the first receiving portion 450, the second receiving portion 460, and the third receiving portion. Compared with the case where the part 470 is not provided, the possibility that the stop solution 96, the substrate solution 95, the second washing solution 94, and the labeled antibody solution 93 are mixed can be reduced. That is, by providing the first receiving portion 450, the second receiving portion 460, and the third receiving portion 470, if R3, R4, R5, and R6 are larger than R2, respectively, under the conditions that make each reagent 9 difficult to mix. The condition is good. For this reason, the difference in inclination angle between the first wall surface 501 and the second wall surface 502 and between each of the third to sixth wall surfaces 503 to 506 and the second wall surface 502 can be increased. Therefore, it is possible to reduce the possibility that the reagent 9 flows out downstream at an unexpected timing, and to reduce the possibility that the inspection accuracy is lowered.

  Further, it is assumed that the first cleaning liquid 92 moves to the reaction unit 380 with the inspection liquid 91 remaining in the reaction unit 380. In this case, the amount of the test liquid 91 contained in the first cleaning liquid 92 is larger than when the first cleaning liquid 92 moves to the reaction section 380 after the test liquid 91 flows downstream from the reaction section 380. Therefore, there is a possibility that the test liquid 91 in the reaction unit 380 reacts with the labeled antibody solution 93 and the test accuracy is lowered. In the present embodiment, as shown in the above formula (2), the inclination angle between the second wall surface 502 and the seventh wall surface 507 has a relationship of R2 ≧ R7. Therefore, as shown in FIG. 7, the test chip 2 causes the test liquid 91 that has flowed to the reaction unit 380 before or simultaneously with the first cleaning liquid 92 to flow downstream from the first cleaning liquid holding unit 320. Can flow downstream. For this reason, compared with the case where R2 <R7, the possibility that the first cleaning liquid 92 and the test liquid 91 are mixed in the reaction unit 380 can be reduced. Therefore, the possibility that the test liquid 91 remains in the reaction unit 380 can be reduced, and the possibility that the labeled antibody solution 93 and the test liquid 91 react in the reaction unit 380 can be reduced. Therefore, possibility that inspection accuracy will fall can be reduced.

  Further, the first cleaning liquid holding unit 320 is positioned in the formation direction from the test liquid holding unit 310. The first outflow end 521 is located in the formation direction from the first virtual line 511. Therefore, the test liquid 91 flowing out from the test liquid holding unit 310 along the first virtual line 511 is retained in the first cleaning liquid as compared with the case where the first outflow end 521 is above the first virtual line 511. It becomes difficult to flow into the part 320. Therefore, the possibility that the test liquid 91 and the first cleaning liquid 92 are mixed can be reduced. Further, the labeled antibody solution holding unit 330 is positioned in the forming direction from the first cleaning solution holding unit 320. The third outflow end portion 523 is located in the formation direction from the second imaginary line 512. For this reason, compared with the case where the third outflow end 523 is above the second imaginary line 512, the first washing liquid 92 flowing out from the first washing liquid holding unit 320 along the second imaginary line 512 is labeled antibody. It becomes difficult to flow into the liquid holding unit 330. Therefore, the possibility that the first cleaning solution 92 and the labeled antibody solution 93 are mixed can be reduced. Therefore, possibility that inspection accuracy will fall can be reduced.

  In the embodiment, the formation direction is an example of “one direction” in the present invention. In addition, this invention is not limited to said embodiment, A various change is possible. For example, the first outflow end 521 may be positioned on the first virtual line 511 or in the upward direction from the first virtual line 511. The third outflow end 523 may be positioned on the second imaginary line 512 or above the second imaginary line 512. In addition, the first cleaning liquid holding unit 320 may be positioned above the test liquid holding unit 310.

  Moreover, the 1st receiving part 450, the 2nd receiving part 460, and the 3rd receiving part 470 do not need to be provided. In this case, the stop liquid holding unit 360 may be connected to the substrate solution holding unit 350. The substrate solution holding unit 350 may be connected to the second cleaning liquid holding unit 340. The second cleaning liquid holding unit 340 may be connected to the labeled antibody liquid holding unit 330.

  Further, the inclination angle between the second wall surface 502 and the seventh wall surface 507 may be in a relationship of R2 <R7. The inclination angles of the second wall surface 502, the fourth wall surface 504, the fifth wall surface 505, and the sixth wall surface 506 may be in a relationship of R4, R5, R6 <R2. The stop liquid holding unit 360 and the substrate solution holding unit 350 may be provided upstream of the test liquid holding unit 310 or upstream of the first cleaning liquid holding unit 320.

  In addition, as shown in FIG. 5N, the stop solution 96 is mixed with the substrate solution 95 in the measurement unit 390. However, the stop solution 96 may be mixed upstream of the measurement unit 390.

2 Test chip 91 Test liquid 92 First cleaning liquid 93 Labeled antibody liquid 94 Second cleaning liquid 95 Substrate solution 96 Stop liquid 310 Test liquid holding part 320 First cleaning liquid holding part 330 Labeled antibody liquid holding part 340 Second cleaning liquid holding part 350 Substrate solution Holding unit 360 Stopping liquid holding unit 370 Common receiving unit 380 Reaction unit 450 First receiving unit 460 Second receiving unit 470 Third receiving unit 501 First wall surface 502 Second wall surface 503 Third wall surface 504 Fourth wall surface 505 Fifth wall surface 506 Sixth wall surface 507 Seventh wall surface 511 First imaginary line 512 Second imaginary line 521 First outflow end 522 Second outflow end 523 Third outflow end 610 First guide channel 620 Second guide channel

Claims (5)

A test liquid holding part which is formed to be recessed in one direction and holds a test liquid containing a test target substance;
A first cleaning liquid holding part that is formed to be recessed in the one direction and holds the first cleaning liquid;
A labeled antibody solution holding part formed to be recessed in the one direction and holding a labeled antibody solution containing an enzyme-labeled antibody;
A common receiving part to which the test liquid holding part and the first cleaning liquid holding part are connected via a first guide channel, and the labeled antibody liquid holding part is connected via a second guide channel;
A reaction part provided downstream of the common receiving part and in which an immobilized antibody is disposed;
A first wall surface that forms the test liquid holding portion and guides the test liquid to the first guide channel;
Forming the first cleaning liquid holding portion, and a second wall surface for guiding the first cleaning liquid to the first guide channel;
Forming the labeled antibody solution holding part, and comprising a third wall surface for guiding the labeled antibody solution to the second guide channel,
An inclination angle R1 that is an angle formed by the first wall surface and the one direction , an inclination angle R2 that is an angle formed by the second wall surface and the one direction, and an angle formed by the third wall surface and the one direction. The inclination angle R3 is
R3>R2> R1
An inspection chip that having a relationship,
The inspection chip extends in the extending direction of each of the first wall surface, the second wall surface, and the third wall surface with respect to the direction in which the centrifugal force acts by revolving around a vertical axis separated from the inspection chip. The test liquid held in the test liquid holding part, the first cleaning liquid held in the first cleaning liquid holding part, by switching by rotation around a horizontal axis extending in the direction of the speed when revolving, And the test | inspection chip | tip characterized by flowing the said labeled antibody liquid hold | maintained at the said labeled antibody liquid holding | maintenance part into the said common receiving part in order . A stop liquid holding part formed to be recessed in the one direction and holding a stop liquid for stopping the progress of the enzyme reaction;
A substrate solution holding unit provided downstream of the stop solution holding unit, formed to be recessed in the one direction, and holding a substrate solution for an enzyme reaction;
2. The test chip according to claim 1, wherein the labeled antibody solution holding unit is provided downstream of the substrate solution holding unit. A second cleaning liquid holding part that is provided downstream of the substrate solution holding part and upstream of the labeled antibody liquid holding part, is formed to be recessed in the one direction, and holds a second cleaning liquid;
A first receiving part provided downstream of the stop liquid holding part and upstream of the substrate solution holding part;
A second receiving part provided downstream of the substrate solution holding part and upstream of the second cleaning liquid holding part;
A third receiving part provided downstream of the second washing liquid holding part and upstream of the labeled antibody liquid holding part;
Forming the stop liquid holding part, and a fourth wall surface for guiding the stop liquid to the first receiving part;
Forming a substrate solution holding portion, and a fifth wall surface for guiding the substrate solution to the second receiving portion;
Forming the second cleaning liquid holding part, and comprising a sixth wall surface for guiding the second cleaning liquid to the third receiving part,
An inclination angle R1 that is an angle formed by the first wall surface and the one direction , an inclination angle R2 that is an angle formed by the second wall surface and the one direction, and an angle formed by the third wall surface and the one direction. the inclination angle R3 is, an inclination angle R4 is a angle between the direction and the fourth wall, and the inclination angle R5 is a angle between the direction and the fifth wall, and the sixth wall R3 is an inclination angle R 6 is the angle between the direction, R4, R5, R6> R2 > R1
Have a relationship,
By switching the extending direction of each of the fourth wall surface, the fifth wall surface, and the sixth wall surface with respect to the direction in which the centrifugal force is applied by the revolution, the labeled antibody solution holding unit holds the switch. When the labeled antibody solution flows into the common receiving portion, the stop solution held in the stop solution holding portion is caused to flow into the first receiving portion and the substrate solution held in the substrate solution holding portion. It was introduced into the second receiving section, the inspection chip according to claim 2, characterized in Rukoto the second cleaning liquid retained in the second washing solution holding portion to flow into the third receiving portion. Forming a reaction part, and comprising a seventh wall surface for guiding a liquid downstream in the reaction part,
An inclination angle R2 that is an angle between the second wall surface and the one direction, and an inclination angle R7 that is an angle between the seventh wall surface and the one direction are:
R2 ≧ R7
Have a relationship,
Before the first cleaning liquid held in the first cleaning liquid holding part flows into the common receiving part by switching the extending direction of the seventh wall surface by the rotation with respect to the direction in which the centrifugal force acts by the revolution. At the same time, the inspection chip according to any one of claims 1 to 3 , wherein the liquid held in the reaction section is guided downstream . The first cleaning liquid holding part is located in the one direction from the inspection liquid holding part,
The labeled antibody solution holding unit is located in the one direction from the first cleaning solution holding unit,
The first outflow end portion on the downstream side in the first cleaning liquid holding portion is in the one direction from the first imaginary line drawn perpendicularly to the first wall surface from the second outflow end portion on the downstream side in the inspection liquid holding portion. Located in
A third outflow end portion on the downstream side in the labeled antibody solution holding portion is located in the one direction from a second imaginary line drawn perpendicularly from the first outflow end portion to the second wall surface. The inspection chip according to claim 1.

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