JP2014010043A - Inspection system, inspection object acceptor and inspection method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an inspection system for easily acquiring a proper inspection result from mixed liquid of a sample generated in an inspection object acceptor and reagent, an inspection object acceptor to be used in the inspection system and an inspection method.SOLUTION: A liquid circuit 25 of an inspection chip 2 includes: a second surplus part 118 in which separated components of a sample 10 are stored; a first mixing section 120 in which first mixed liquid is stored; and a second mixing section 140 in which second mixed liquid is stored. In the second surplus section 118, an optical path is formed on a liquid surface of the stored sample 10 or a lower side thereof. In the first mixing section 120, an optical path is formed on a liquid surface of the stored first mixed liquid or a lower side thereof. In the second mixing section 140, an optical path is formed on a liquid surface of the stored second mixed liquid or a lower side thereof.

Description

  The present invention relates to an inspection object receiver for performing chemical, medical, and biological inspections of an inspection object, and rotating the inspection object receptor to apply a centrifugal force to the inspection object to perform the inspection. The present invention relates to an inspection system and an inspection method performed by the inspection system.

  2. Description of the Related Art Conventionally, there is known an inspection apparatus that inspects biological substances, chemical substances, and the like by centrifuging an inspection target receptacle called a microchip or an inspection chip (for example, see Patent Document 1). Patent Document 1 discloses an information measuring device that rotates a microchannel structure and sequentially measures information from a measuring unit.

JP 2003-149253 A

  However, in the conventional inspection apparatus, there has been a possibility that an accurate inspection cannot be performed because an appropriate separation liquid cannot be obtained from the mixed liquid of the specimen and the reagent generated in the inspection target receptacle. Specifically, for example, when blood as a specimen is centrifuged to produce a plasma supernatant, blood cell components damaged by centrifugal force may be mixed into the supernatant. There is a problem that proper test results cannot be obtained even if a reagent is mixed with the supernatant mixed with impurities.

  An object of the present invention is to provide a test system that can easily obtain a proper test result from a mixed solution of a specimen and a reagent generated in a test target receiver, and a test target receiver and a test method used therefor. To do.

  An inspection system according to the first aspect of the present invention includes an inspection target receptacle into which a liquid specimen and a reagent are injected, and an inspection apparatus that centrifuges the specimen by rotating the inspection target receptacle. The test object receiver includes a plate-like main body having a predetermined thickness, and a liquid circuit that extends in a direction perpendicular to the thickness direction in the main body and allows the specimen and the reagent to flow. The liquid circuit is capable of storing the sample, the detection unit for detecting the stored sample, the liquid mixture of the sample and the reagent, and the stored liquid mixture being detected A mixing unit, wherein the inspection apparatus is configured such that the first axis provided apart from the inspection target receptacle and the liquid circuit extend along the same plane. One axis and the first axis Receptor holding means that holds the second axis rotatably, a light source that emits light, and a detector that receives the light emitted from the light source by forming an optical path between the light source and the light source. A first centrifuge that causes the specimen to flow into the detection unit by controlling rotation of the test object receiver held by the receiver holding means about the first axis and the second axis; The specimen and the reagent are mixed by controlling the rotation of the receptor to be inspected held by the receiver holding means about the first axis and the second axis, and the mixed liquid is A second centrifuge for flowing into the mixing unit; and the first axis and the second of the test subject receiver held by the receiver holding unit in a state where at least the specimen is stored in the detection unit. Controls rotation about the axis Thus, when the detection unit is arranged on the optical path by the first movement unit that moves the detection unit to a position arranged on the optical path, the first detection unit detects the detection unit. Specimen information acquisition means for acquiring specimen information that is information relating to the specimen based on the amount of received light, and the examination held by the receiver holding means in a state where at least the mixed liquid is stored in the mixing section A second moving means for moving the mixing unit to a position disposed on the optical path by controlling rotation of at least one of the first axis and the second axis of the target receiver; and When the mixing unit is disposed on the optical path by two moving means, a mixed liquid that acquires mixed liquid information that is information on the mixed liquid based on the amount of light received detected by the detector A test result acquisition unit that acquires a test result of the sample based on the information acquisition unit, the sample information acquired by the sample information acquisition unit, and the liquid mixture information acquired by the liquid mixture information acquisition unit With.

  The test object receiver according to the second aspect of the present invention is injected with a liquid sample and reagent, and is rotated about a predetermined first axis by a test apparatus to apply a centrifugal force. A test object receptacle in which the direction of the centrifugal force is changed by being rotated around a second axis different from the one axis, and extends along the direction of the centrifugal force, and has a predetermined thickness. And a liquid circuit that extends in a direction perpendicular to the thickness direction of the main body and allows the liquid to flow. The liquid circuit is capable of storing the sample, and the stored sample And a mixing unit that can store the mixed liquid of the sample and the reagent and that detects the stored mixed liquid. When rotated to the first angle around two axes, An optical path extending in a direction intersecting with the direction of the centrifugal force is formed on the liquid surface of the specimen stored in the detection unit or on the lower side thereof, and the mixing unit is configured so that the test target receptacle is centered on the second axis. When rotated to the second angle, the optical path is formed at or below the liquid surface of the mixed liquid stored in the mixing unit.

  An inspection method according to a third aspect of the present invention includes an inspection target receptacle into which a liquid specimen and a reagent are injected, and an inspection apparatus that centrifuges the specimen by rotating the inspection target receptacle. An inspection method executed in the system, wherein the inspection object receiver has a plate-like main body portion having a predetermined thickness, and extends in a direction perpendicular to the thickness direction in the main body portion, and the specimen and the reagent flow A liquid circuit capable of storing the specimen, a detection unit that detects the stored specimen, and a liquid mixture of the specimen and the reagent that can be stored. A mixing unit for detecting a liquid mixture, wherein the inspection apparatus is configured such that a first axis provided apart from the inspection target receptacle and the liquid circuit are arranged on the same plane. In the extended state, the first shaft And a receiver holding means for rotatably holding a second axis different from the first axis, a light source for irradiating light, and an optical path formed between the light source and being irradiated from the light source A detector that receives light, and the inspection method controls rotation about the first axis and the second axis of the inspection target receiver held by the receiver holding means, By controlling the rotation about the first axis and the second axis of the test object receiver held by the receiver holding means, the first centrifugation step for flowing the specimen into the detection unit, A second centrifugation step of mixing the specimen and the reagent and allowing the mixed solution to flow into the mixing section; and at least the specimen is retained by the detection section and held by the receiver holding means The test object receiver A first movement step of moving the detection unit to a position disposed on the optical path by controlling rotation about one axis and the second axis, and the detection unit causes the light to move to the position arranged on the optical path. When arranged on the road, based on the amount of received light detected by the detector, a sample information acquisition step for acquiring sample information that is information about the sample, and at least the mixed solution is stored in the mixing unit In the state, the mixing unit is disposed on the optical path by controlling the rotation of the inspection target receiver held by the receiver holding means around the first axis and the second axis. A second moving step for moving to a position to be moved, and when the mixing unit is arranged on the optical path by the second moving step, the received light amount detected by the detector Based on the above, the liquid mixture information acquisition step for acquiring the liquid mixture information that is information on the liquid mixture, the sample information acquired by the sample information acquisition step, and the mixture acquired by the liquid mixture information acquisition step A test result acquiring step for acquiring a test result of the sample based on the liquid information.

  According to the first to third aspects of the present invention, based on the sample information acquired from the sample in the detection unit and the liquid mixture information acquired from the mixed liquid of the reagent and the sample, an appropriate inspection is performed by the inspection apparatus. You can get the result. Further, by rotating the test subject receiver about the first axis and the second axis, not only a mixed liquid of the specimen and the reagent is generated, but also the detection unit and the mixing unit are connected to one light source and the detector. It is possible to measure light with a common optical path between them. In other words, the sample in the detection unit can be measured by the optical path for measuring the mixed liquid. Therefore, it is not necessary to provide an optical path for measuring the sample in the detection unit in the inspection apparatus, and thus an appropriate inspection result can be easily obtained by the inspection apparatus.

It is a rear view of the inspection apparatus 1 in which the inspection chip 2 is in a steady state. It is a rear view of the test | inspection apparatus 1 in which the test | inspection chip 2 exists in a displacement state. It is a top view of the inspection apparatus 1 shown in FIG. It is the perspective view which looked at the inspection apparatus 1 shown in FIG. 1 from diagonally upward. It is a front view of the test | inspection chip 2. FIG. 3 is a block diagram showing an electrical configuration of a control device 90. FIG. 6 is a diagram showing a data configuration of a correction table 8. FIG. 4 is a flowchart of main processing executed by the inspection apparatus 1. It is a front view of the test | inspection chip 2 revolved by 90 autorotation angles. It is a front view of the test | inspection chip 2 revolved by the autorotation angle of 0 degree. It is a front view of the test | inspection chip 2 revolved by 90 autorotation angles. It is a front view of the test | inspection chip 2 revolved by the autorotation angle of 45 degree | times. It is a front view of the test | inspection chip 2 which an optical path passes along the 1st mixing part 120. FIG. It is a front view of the test | inspection chip 2 revolved by the autorotation angle of 0 degree. It is a front view of the test | inspection chip 2 which an optical path passes along the 2nd mixing part 140. FIG. It is a front view of the test | inspection chip 2 which an optical path passes along the 2nd surplus part 118. FIG. It is a front view of the test | inspection chip 2 of the 1st modification before a centrifugation process. It is a front view of the test | inspection chip 2 of the 1st modification after centrifugation. It is a front view of the test | inspection chip 2 of the 2nd modification before centrifugation. It is a front view of the test | inspection chip 2 of the 2nd modification after centrifugation.

  Embodiments of the present invention will be described with reference to the drawings. The drawings to be referred to are used to explain technical features that can be adopted by the present invention, and are merely illustrative examples.

  A schematic structure of the inspection system 3 will be described with reference to FIGS. 1 and 2. The inspection system 3 of the present embodiment includes an inspection chip 2 that can accommodate a liquid to be inspected (hereinafter referred to as a specimen) and a liquid mixed with the specimen (hereinafter referred to as a reagent), and the inspection chip 2. And an inspection apparatus 1 that performs inspection using the same. The inspection apparatus 1 can apply a centrifugal force to the inspection chip 2 by rotation about a vertical axis that is separated from the inspection chip 2. The inspection apparatus 1 can switch the direction of the centrifugal force applied to the inspection chip 2 (hereinafter referred to as the centrifugal direction) by rotating the inspection chip 2 about the horizontal axis.

  With reference to FIGS. 1-4, the detailed structure of the inspection apparatus 1 is demonstrated. In the following description, the upper, lower, right, left, front side, and back side of FIGS. 1 and 2 are respectively the upper, lower, right, left, rear, and front sides of the inspection apparatus 1. To do. The upper, lower, right, left, front side, and back side of FIG. 3 are the front, lower, right, left, upper, and lower sides of the inspection apparatus 1, respectively. For easy understanding, FIG. 1 and FIG. 2 show the upper housing 30 with a virtual line, and FIG. 3 shows a state where the top plate of the upper housing 30 is removed. In FIG. 4, only the upper housing 30 and the turntable 33 among the members installed on the upper plate 32 are illustrated.

  As shown in FIGS. 1 to 4, the inspection apparatus 1 includes an upper housing 30, a lower housing 31, a turntable 33, an angle changing mechanism 34, a control device 90, and the like. The lower housing 31 is provided with a drive mechanism for rotating the turntable 33 around a vertical axis. The turntable 33 is a disk-shaped rotating body that is provided on the upper surface side of the lower housing 31 and holds the inspection chip 2 upward. The angle changing mechanism 34 is a drive mechanism that is provided on the turntable 33 and rotates the inspection chip 2 around the horizontal axis. The upper housing 30 is fixed to the upper side of the lower housing 31, and the measurement unit 7 that performs optical measurement on the test chip 2 is provided inside. The control device 90 is a controller that controls centrifugal processing, measurement processing, and the like of the inspection device 1.

  The detailed structure of the lower housing 31 will be described. The lower housing 31 has a box-shaped frame structure in which frame members are combined. On the upper surface of the lower housing 31, an upper plate 32 that is a rectangular plate material in plan view is provided. A turntable 33 is rotatably provided above the upper plate 32. Inside the lower housing 31, a mechanism for rotating the turntable 33 is provided as follows.

  A spindle motor 35 that supplies a driving force for rotating the turntable 33 is installed on the left side in the lower housing 31. A shaft 36 of the main shaft motor 35 protrudes upward, and a pulley 37 is fixed. A vertical main shaft 57 extending upward from the inside of the lower housing 31 is provided at the center of the lower housing 31 in plan view. The main shaft 57 passes through the upper plate 32 and protrudes above the lower housing 31. The upper end portion of the main shaft 57 is connected to the center portion of the turntable 33 in plan view.

  A support member 53 that is a holding metal fitting through which the main shaft 57 passes is provided directly below the upper plate 32. The main shaft 57 is rotatably held by the support member 53. A pulley 38 is fixed below the support member 53 on the main shaft 57. A belt 39 is stretched over the pulleys 37 and 38. When the main shaft motor 35 rotates the shaft 36, the driving force is transmitted to the main shaft 57 via the pulley 37, the belt 39 and the pulley 38. At this time, the turntable 33 rotates around the main shaft 57 in conjunction with the rotation of the main shaft 57.

  A guide rail 56 that extends vertically from the bottom surface of the lower housing 31 to the lower surface of the upper plate 32 is provided on the right side in the lower housing 31. The T-shaped plate 48, which is a T-shaped plate-shaped connection fitting, is movable in the vertical direction within the lower housing 31 along the guide rail 56. A horizontally long groove 80 is formed on the front side of the T-shaped plate 48 (the back side in FIG. 1 and FIG. 2).

  The aforementioned main shaft 57 is a cylindrical body having a hollow inside. The inner shaft 40 is a vertical shaft that can move in the vertical direction inside the main shaft 57. An upper end portion of the inner shaft 40 passes through the main shaft 57 and is connected to a rack gear 43 described later. A bearing 55 fixed to the middle frame member 52 is provided below the main shaft 57. A bearing 41 is provided at the left end of the T-shaped plate 48. Inside the bearing 41, the lower end portion of the inner shaft 40 is rotatably held.

  On the front side of the T-shaped plate 48, a stepping motor 51 for moving the T-shaped plate 48 up and down is fixed by a fixture (not shown). The shaft 58 of the stepping motor 51 protrudes toward the rear side (the front side in FIG. 1 and FIG. 2), and a disc-shaped cam plate 59 is fixed to the tip. A cylindrical projection 70 is provided on the rear surface of the cam plate 59. Since the tip of the protrusion 70 is inserted into the groove 80 described above, the protrusion 70 can slide in the groove 80. When the stepping motor 51 rotates the shaft 58, the projection 70 moves up and down in conjunction with the rotation of the cam plate 59. At this time, the T-shaped plate 48 moves up and down along the guide rail 56 in conjunction with the protrusion 70 inserted in the groove 80.

  The detailed structure of the angle changing mechanism 34 will be described. The angle changing mechanism 34 has an L-shaped plate 60 that is a pair of L-shaped plate-shaped connecting brackets fixed to the upper surface of the turntable 33. Each L-shaped plate 60 extends upward from a base portion fixed in the vicinity of the center of the turntable 33, and its upper end portion extends outward in the radial direction of the turntable 33. A rack gear 43 fixed to the inner shaft 40 is provided between the pair of L-shaped plates 60. The rack gear 43 is a vertically long metal plate-like member, and gears are carved on both end faces.

  On the front end side in the extending direction of each L-shaped plate 60, a horizontal support shaft 46 included in the gear 45 is rotatably supported. Since the support shaft 46 is fixed to the inspection chip 2 via a mounting holder (not shown), the inspection chip 2 also rotates about the support shaft 46 in conjunction with the rotation of the gear 45. Between the gear 45 and the rack gear 43, a pinion gear 44 supported by an L-shaped plate 60 so as to be rotatable about a horizontal axis is interposed. The pinion gear 44 meshes with the gear 45 and the rack gear 43, respectively. In conjunction with the vertical movement of the rack gear 43, the pinion gear 44 and the gear 45 are driven to rotate, and the inspection chip 2 rotates about the support shaft 46.

  In the present embodiment, as the main shaft motor 35 rotates and drives the turntable 33, the inspection chip 2 rotates about the main shaft 57 (that is, the vertical axis), and centrifugal force is applied to the inspection chip 2. . The rotation around the vertical axis of the inspection chip 2 is called “revolution”. On the other hand, as the stepping motor 51 moves the inner shaft 40 up and down, the inspection chip 2 rotates about the support shaft 46 (that is, the horizontal axis), and the centrifugal direction acting on the inspection chip 2 changes relatively. . The rotation around the horizontal axis of the inspection chip 2 is referred to as “rotation”.

  When the T-shaped plate 48 is lowered to the lowest end of the movable range (see FIG. 1), the rack gear 43 is also lowered to the lowest end of the movable range. At this time, the inspection chip 2 is in a state where the rotation angle is “0 degree” (hereinafter referred to as a steady state). On the other hand, in a state where the T-shaped plate 48 is raised to the uppermost end of the movable range (see FIG. 2), the rack gear 43 is also raised to the uppermost end of the movable range. At this time, the inspection chip 2 is rotated from the steady state around the horizontal axis by 180 degrees. In the present embodiment, for example, the angular width at which the test chip 2 can rotate is 180 degrees from the steady state where the rotation angle is 0 degrees.

  The detailed structure of the upper housing 30 will be described. The upper housing 30 has a box-like frame structure in which frame members are combined, and is installed on the upper left side of the upper plate 32. More specifically, the upper housing 30 is provided outside the range in which the inspection chip 2 is rotated as viewed from the rotation center of the turntable 33 (that is, the main shaft 57). The upper housing 30 has an opposing wall 81 that extends in an arc shape in plan view on the outer peripheral side of the turntable 33.

  The measurement unit 7 provided in the upper housing 30 includes a light source 71 that emits measurement light and an optical sensor 72 that detects the measurement light emitted from the light source 71. The light source 71 and the optical sensor 72 are disposed on both the front and rear sides of the turntable 33 outside the rotation range of the inspection chip 2. The light source 71 includes a light emitting unit 71 </ b> A that emits measurement light toward the rear side of the inspection apparatus 1. The optical sensor 72 has a light receiving portion 72A that receives measurement light emitted from the front side of the inspection apparatus 1. In the facing wall 81, exposure ports 85 and 86 for exposing the light emitting portion 71A and the light receiving portion 72A to the outside of the upper housing 30 are formed. In the present embodiment, the left side position of the main shaft 57 in the reciprocable range of the inspection chip 2 is a measurement position at which the inspection chip 2 is irradiated with measurement light. When the inspection chip 2 is at the measurement position, the optical path connecting the light source 71 and the optical sensor 72 intersects the front and rear surfaces of the inspection chip 2 perpendicularly in plan view.

  With reference to FIG. 5, the detailed structure of the test | inspection chip 2 is demonstrated. In the following description, the upper, lower, right, left, front side, and back side of FIG. 5 are respectively the upper, lower, right, left, front, and rear sides of the inspection chip 2. The inspection chip 2 has a square shape when viewed from the front, and mainly includes a transparent synthetic resin plate 20 having a predetermined thickness. A liquid circuit 25 is formed on the front surface of the plate member 20 so that the liquid sealed in the inspection chip 2 can flow. The liquid circuit 25 is a recess formed at a predetermined depth on the front side of the plate member 20 and extends in a direction orthogonal to the thickness direction of the plate member 20. The front surface of the plate member 20 is sealed with a sheet (not shown) made of a transparent synthetic resin thin plate.

  The liquid circuit 25 includes a first storage unit 110, a separation unit 112, a first surplus unit 114, a first fixed amount unit 116, a second surplus unit 118, a first mixing unit 120, a second storage unit 130, a second It has a quantification unit 132, a third surplus unit 134, a second mixing unit 140, a third storage unit 150, a third quantification unit 152, a fourth surplus unit 154, and the like. Although not shown, the sheet is formed with openings (injection ports) for injecting liquid into the first to third reservoirs 110, 130, and 150, respectively. After the liquid is injected into the first to third reservoirs 110, 130, and 150, each inlet is sealed with a sealing patch (not shown) attached.

  The first to third reservoirs 110, 130, and 150 are portions into which the specimen 10, the first reagent 11, and the second reagent 12 are injected and stored, respectively. The first to third storage portions 110, 130, and 150 are formed side by side in the left-right direction along the upper side portion 21 of the test chip 2. Of the first to third reservoirs 110, 130, 150, the first reservoir 110 is closest to the left side 23 of the test chip 2, the third reservoir 150 is closest to the right side 22 of the test chip 2, The two reservoirs 130 are located between the first reservoir 110 and the third reservoir 150.

  A first supply path 111 is formed from the first reservoir 110 toward the lower side. A separation unit 112 is provided below the first supply path 111. The separation unit 112 is a part for centrifuging the specimen 10, and is a rectangular recess that opens upward in a front view. A first passage 113 and a second passage 115 extend from the portion where the first supply passage 111 and the separation portion 112 communicate with each other to the left and right sides. The first passage 113 is connected to the lower left end portion of the first supply path 111 and the upper left end portion of the separation portion 112, and extends to the first surplus portion 114 provided on the lower left side of the separation portion 112. The first surplus portion 114 is a portion in which the specimen 10 overflowing from the separation portion 112 is stored, and is a rectangular recess extending in the lower left direction from the lower end portion of the first passage 113 in a front view.

  The second passage 115 is connected to the lower right end portion of the first supply path 111 and the upper right end portion of the separation portion 112, and extends to the upper side of the first fixed amount portion 116 provided on the lower right side of the separation portion 112. . The first quantification unit 116 is a part that quantifies the separated component 13 (see FIG. 9) of the specimen 10 that has been centrifuged by the separation unit 112, and is a recess that opens upward in a front view. A third passage 117 and a fourth passage 119 extend from the portion where the second passage 115 and the first metering unit 116 communicate with each other to the left and right sides. The third passage 117 is connected to the lower left end portion of the second passage 115 and the upper left end portion of the first fixed amount portion 116, up to the second surplus portion 118 provided at the lower left of the first fixed amount portion 116. It extends. The second surplus portion 118 is a portion in which the separated component 13 overflowing from the first fixed amount portion 116 is stored, and is a rectangular recess extending in the lower left direction from the lower end portion of the third passage 117 in a front view. . The fourth passage 119 is connected to the lower right end portion of the second passage 115 and the upper right end portion of the first fixed amount portion 116, and will be described later in a first mixing portion 120 provided below the first fixed amount portion 116. It extends to.

  A second supply path 131 is formed from the second reservoir 130 toward the lower side. Below the second supply path 131, a second fixed amount portion 132 is provided. The 2nd fixed_quantity | quantitative_assay part 132 is a site | part which quantifies the 1st reagent 11, and is a recessed part opened upwards by front view. The fifth passage 133 and the sixth passage 135 extend to the left and right sides from the portion where the second supply passage 131 and the second fixed portion 132 communicate. The fifth passage 133 is connected to the lower left end portion of the second supply path 131 and the upper left end portion of the second fixed amount portion 132 and extends to the third surplus portion 134 provided at the lower left portion of the second fixed amount portion 132. ing. The third surplus part 134 is a part where the first reagent 11 overflowing from the second quantification part 132 is stored, and is a rectangular recess extending rightward from the lower end of the fifth passage 133 in a front view. .

  The sixth passage 135 is connected to the lower right end portion of the second supply path 131 and the upper right end portion of the second fixed amount portion 132, to the upper side of the first mixing portion 120 provided at the lower right side of the second fixed amount portion 132. It extends. The first mixing unit 120 is a part where the separated component 13 quantified by the first quantification unit 116 and the first reagent 11 quantified by the second quantification unit 132 are mixed, and opens upward in a front view. It is a recessed part to do. A mixed solution of the separation component 13 and the first reagent 11 is referred to as a first mixed solution 15 (see FIG. 12). The fourth passage 119 and the seventh passage 136 described above extend from the portion where the sixth passage 135 and the first mixing unit 120 communicate with each other to the left and right sides. The seventh passage 136 is connected to the lower right end portion of the sixth passage 135 and the upper right end portion of the first mixing portion 120, and extends to the second mixing portion 140 described later provided on the upper right side of the first mixing portion 120. ing.

  A third supply path 151 is formed from the third reservoir 150 toward the lower side. A third fixed amount unit 152 is provided below the third supply path 151. The 3rd fixed_quantity | quantitative_assay part 152 is a site | part which quantifies the 2nd reagent 12, and is a recessed part opened upwards by front view. The eighth passage 153 and the mixing chamber 155 extend to the left and right sides from the portion where the third supply path 151 and the third metering unit 152 communicate with each other. The eighth passage 153 is connected to the lower left end portion of the third supply passage 151 and the upper left end portion of the third fixed amount portion 152, and extends to the fourth surplus portion 154 provided at the lower left portion of the third fixed amount portion 152. ing. The fourth surplus portion 154 is a portion in which the second reagent 12 overflowing from the third fixed amount portion 152 is stored, and is a rectangular recess extending rightward from the lower end portion of the eighth passage 153 in front view. .

  The mixing chamber 155 is connected to the lower right end portion of the third supply path 151 and the upper right end portion of the third fixed amount portion 152, and extends to the upper side of the second mixing portion 140 provided on the lower right side of the third fixed amount portion 152. ing. In the mixing chamber 155, the second mixing unit 140 is a part where the first mixed liquid 15 generated by the first mixing unit 120 and the second reagent 12 quantified by the third quantification unit 152 are mixed. The second mixing unit 140 is a rectangular recess that opens the mixed solution of the first mixed solution 15 and the second reagent 12 generated in the mixing chamber 155 upward in a front view. A mixed solution of the first mixed solution 15 and the second reagent 12 is referred to as a second mixed solution 16 (see FIG. 14).

  The support shaft 46 extending from the L-shaped plate 60 is vertically connected to the center of the back surface of the plate member 20 and supports the test chip 2 in a state where the main shaft 57 and the liquid circuit 25 extend along the same surface. As the support shaft 46 rotates, the inspection chip 2 rotates in the counterclockwise direction around the support shaft 46 (axis O in FIG. 5) when viewed from the front. When the inspection chip 2 is in a steady state (see FIG. 5), the upper side portion 21 and the lower side portion 24 are orthogonal to the gravity direction B, the right side portion 22 and the left side portion 23 are parallel to the weight direction B, and the left side portion 23 is It is arranged closer to the main shaft 57 than the right side portion 22.

  In the inspection chip 2, the first surplus part 114, the second surplus part 118, the first mixing part 120, and the second mixing part 140 are formed at substantially equal distances from the axis O in a front view. When the inspection chip 2 is arranged at the measurement position in a steady state, the optical path connecting the light source 71 and the optical sensor 72 passes through the second mixing unit 140 vertically in a plan view. Therefore, when the inspection chip 2 at the measurement position is rotated about the axis O, an arc-shaped trajectory passing through the first surplus part 114, the second surplus part 118, the first mixing part 120, and the second mixing part 140 ( The optical path is relatively moved by a dotted arrow in FIG.

  The electrical configuration of the control device 90 will be described with reference to FIG. The control device 90 includes a CPU 91 that performs main control of the inspection device 1, a RAM 92 that temporarily stores various data, and a ROM 93 that stores a control program. Connected to the CPU 91 are an operation unit 94 for inputting instructions to the control device 90, an HDD 95 which is a hard disk device for storing various data and programs, a display 96 for displaying various information, and the like. The HDD 95 stores a correction table 8 described later.

  A revolution controller 97, a rotation controller 98, a measurement controller 99, and the like are connected to the CPU 91. The revolution controller 97 controls the revolution of the inspection chip 2 by rotationally driving the spindle motor 35. The revolution controller 97 can control the number of revolutions and the revolution position of the inspection chip 2 per unit time. The rotation controller 98 controls the rotation of the inspection chip 2 by rotating the stepping motor 51. The rotation controller 98 can control the rotation angle of the inspection chip 2. The measurement controller 99 performs light measurement described later by performing light emission control of the light source 71 and detection control of the optical sensor 72.

  The correction table 8 will be described with reference to FIG. The correction table 8 is used to correct the inspection results of the first mixed liquid 15 and the second mixed liquid 16. When the sample 10 is blood, hemolysis of the sample 10 is one of the factors that cause fluctuations in the test values of the first mixed solution 15 and the second mixed solution 16. In the present embodiment, the hemolysis index and the correction value are stored in association with each other in the correction table 8 in order to correct the influence of the hemolysis of the specimen 10 on the test result.

  The hemolysis index is a numerical value that expresses the hemolysis level of the specimen 10 based on the presence or absence of the color tone (hemolysis, jaundice, emulsion, etc.) of the separation component 13 and its color tone. The larger the hemolysis index, the greater the degree of hemolysis of the separated component 13. The correction value is a numerical value for correcting the hemoglobin concentration detected from the first mixed solution 15 and the second mixed solution 16 that is associated with the hemolysis index. In the correction table 8, a plurality of correction values are set stepwise according to the hemolysis index so that the decrease in the hemoglobin concentration detected from the test object increases as the hemolysis index increases.

  An inspection method using the inspection apparatus 1 will be described with reference to FIGS. The user attaches the inspection chip 2 to the spindle 46 and inputs a process start command from the operation unit 94. As a result, the CPU 91 executes the following main process (FIG. 8) based on the control program stored in the ROM 93. The inspection apparatus 1 can process two inspection chips 2 at the same time, but for the convenience of explanation, a procedure for inspecting one inspection chip 2 will be described below.

  As shown in FIG. 8, in the main process of the inspection apparatus 1, the first mixed liquid 15 is generated by the following rotation control of the inspection chip 2 (S1). First, as shown in FIG. 9, the rotation controller 98 rotates the inspection chip 2 in a steady state (that is, the rotation angle “0 degree”) 90 degrees counterclockwise when viewed from the front, and the rotation angle is set to “90 degrees. ”. The revolution controller 97 revolves the inspection chip 2 displaced to the rotation angle “90 degrees”. Thereby, the centrifugal force A acts on the test chip 2 from the upper side portion 21 toward the lower side portion 24.

  Due to the action of the centrifugal force A, the specimen 10 stored in the first storage unit 110 flows into the separation unit 112 via the first supply path 111. In the separation unit 112, the sample 10 exceeding a predetermined amount overflows into the first passage 113 and is stored in the first surplus unit 114, whereby the predetermined amount of the sample 10 is quantified. Further, in the separation unit 112 and the first surplus unit 114, the specimen 10 stored in each is centrifuged into a separation component 13 having a small specific gravity and a residual component 14 having a specific gravity larger than that of the separation component 13. In this embodiment, since blood is used as the specimen 10, it is separated into plasma (separation component 13) and blood cells (residual component 14).

  At the same time, the first reagent 11 stored in the second storage unit 130 flows into the second quantitative unit 132 through the second supply path 131. In the second quantification unit 132, a predetermined amount of the first reagent 11 is stored (that is, quantified). The surplus first reagent 11 overflows from the second fixed amount portion 132 to the fifth passage 133 and is stored in the third surplus portion 134. Similarly, the second reagent 12 stored in the third storage unit 150 flows into the third fixed amount unit 152 via the third supply path 151. In the third quantification unit 152, the second reagent 12 exceeding a predetermined amount overflows into the eighth passage 153 and is stored in the fourth surplus portion 154, whereby the predetermined amount of the second reagent 12 is quantified.

  Next, as shown in FIG. 10, the rotation controller 98 rotates the inspection chip 2 with the rotation angle “90 degrees” by 90 degrees clockwise when viewed from the front, and displaces it to a steady state. The revolution controller 97 revolves the inspection chip 2 displaced to a steady state. Thereby, the centrifugal force A acts on the test chip 2 from the left side 23 toward the right side 22. Due to the action of the centrifugal force A, the separation component 13 flows out from the separation portion 112 to the second passage 115, while the residual component 14 remains in the separation portion 112. At the same time, the first reagent 11 quantified by the second quantification unit 132 flows out from the second quantification unit 132 to the sixth passage 135. The second reagent 12 quantified by the third quantification unit 152 flows out from the third quantification unit 152 into the mixing chamber 155.

  Next, as shown in FIG. 11, the rotation controller 98 rotates the inspection chip 2 in a steady state by 90 degrees counterclockwise when viewed from the front, and displaces the rotation angle to “90 degrees”. The revolution controller 97 revolves the inspection chip 2 displaced to the rotation angle “90 degrees”. Due to the action of the centrifugal force A acting from the upper side portion 21 toward the lower side portion 24, the separation component 13 flows from the second passage 115 into the first fixed amount portion 116. In the first quantification unit 116, the separation component 13 exceeding a predetermined amount overflows into the third passage 117 and is stored in the second surplus portion 118, whereby the predetermined amount of the separation component 13 is quantified. At the same time, the first reagent 11 flows from the sixth passage 135 into the first mixing unit 120. The second reagent 12 flows from the mixing chamber 155 into the second mixing unit 140.

  Next, as shown in FIG. 12, the rotation controller 98 rotates the inspection chip 2 having the rotation angle “90 degrees” by 45 degrees clockwise when viewed from the front, and displaces the rotation angle to “45 degrees”. The revolution controller 97 revolves the inspection chip 2 displaced to the rotation angle “45 degrees”. The separated component 13 quantified by the first quantification unit 116 by the action of the centrifugal force A acting from the corner formed by the upper side 21 and the left side 23 toward the corner formed by the right side 22 and the lower side 24. However, it flows into the first mixing unit 120 through the fourth passage 119. In the first mixing unit 120, the separation component 13 and the first reagent 11 are mixed to generate the first mixed solution 15.

  After execution of step S1, the 1st mixing part 120 is moved on an optical path (S3), and the 1st liquid mixture 15 is measured (S5). Specifically, the revolution controller 97 moves the inspection chip 2 to the measurement position. Further, the rotation controller 98 adjusts the rotation angle of the inspection chip 2 so that the optical path passes through the first mixing unit 120. Specifically, as shown in FIG. 13, the rotation angle of the inspection chip 2 at the position where the optical path 9 is formed on the liquid surface of the first mixed liquid 15 stored inside the first mixing unit 120 or on the lower side thereof. Adjust. The inspection apparatus 1 obtains the measurement result (first mixture information) of the first mixture 15 based on the attenuation amount of the measurement light received by the optical sensor 72.

  After execution of step S5, the second liquid mixture 16 is generated by the following rotation control of the inspection chip 2 (S7). First, as shown in FIG. 14, the rotation controller 98 rotates the test chip 2 to a steady state. The revolution controller 97 revolves the inspection chip 2 displaced to the rotation angle “0 degree”. The first mixed solution 15 generated in the first mixing unit 120 flows into the mixing chamber 155 through the seventh passage 136 by the action of the centrifugal force A acting from the left side 23 toward the right side 22. In the mixing chamber 155, the first mixed solution 15 and the second reagent 12 are mixed to generate the second mixed solution 16.

  After execution of step S7, the second mixing unit 140 is moved on the optical path (S9), and the second mixed liquid 16 is measured (S11). Specifically, the revolution controller 97 moves the inspection chip 2 to the measurement position. Further, the rotation controller 98 adjusts the rotation angle of the inspection chip 2 so that the optical path passes through the second mixing unit 140. Specifically, as shown in FIG. 15, the rotation angle of the inspection chip 2 at a position where the optical path 9 is formed on the liquid surface of the second mixed solution 16 stored inside the second mixing unit 140 or on the lower side thereof. Adjust. The inspection device 1 obtains the measurement result (second mixture information) of the second mixture 16 based on the attenuation amount of the measurement light received by the optical sensor 72.

  After execution of step S11, the second surplus part 118 is moved on the optical path (S13), and the separation component 13 is measured (S15). Specifically, the revolution controller 97 moves the inspection chip 2 to the measurement position. Further, the rotation controller 98 adjusts the rotation angle of the inspection chip 2 so that the optical path passes through the second surplus portion 118. Specifically, as shown in FIG. 16, the rotation angle of the test chip 2 is set at a position where the optical path 9 is formed on the liquid surface of the separated component 13 stored in the second surplus portion 118 or on the lower side thereof. adjust. The inspection apparatus 1 obtains the measurement result (specimen information) of the separation component 13 based on the attenuation amount of the measurement light received by the optical sensor 72. The sample information of this embodiment is a hemolytic index of the sample 10.

  In the inspection chip 2 of the present embodiment, the first surplus part 114, the second surplus part 118, the third surplus part 134, the fourth surplus part 154, and the second mixing part 140 flow into the interior, respectively. Even if the test | inspection chip 2 rotates, it has the structure where the liquid which it did not flow out outside easily. Therefore, for example, even when the test chip 2 is rotated in step S13, the separation component 13 does not easily flow out from the second surplus portion 118, and does not easily flow out from the second mixing portion 140 to the second mixed liquid 16. Therefore, the second mixed liquid 16 can be prevented from flowing into the second surplus portion 118 and mixed with the separated component 13, and the separated component 13 can be appropriately detected by the second mixed liquid 16.

  Finally, the test result of the specimen 10 is acquired and displayed on the display 96 (S17). In the present embodiment, with reference to the correction table 8 (see FIG. 7), a correction value corresponding to the sample information (haemolysis index) acquired in step S15 is acquired. Based on this correction value, the first liquid mixture information acquired in step S5 and the second liquid mixture information acquired in step S11 are corrected. The corrected first and second liquid mixture information is displayed as the test result of the specimen 10.

  As described above, the inspection system 3 of the present embodiment includes the inspection chip 2 into which the specimen 10, the first reagent 11, and the second reagent 12 are injected, and the inspection apparatus 1 that centrifuges the specimen 10. The liquid circuit 25 of the test chip 2 stores the second surplus part 118 where the separation component 13 of the specimen 10 is stored, the first mixing part 120 where the first mixed liquid 15 is stored, and the second mixed liquid 16. The second mixing unit 140 is provided. The inspection apparatus 1 includes an angle changing mechanism 34 that holds the inspection chip 2 rotatably around a main shaft 57 and a support shaft 46, a light source 71, and an optical sensor 72.

  In the inspection apparatus 1, a centrifugal force that causes the specimen 10 to flow into the second surplus portion 118 is applied (S1). The sample 10 and the first reagent 11 are mixed, and a centrifugal force that causes the first mixed solution 15 to flow into the first mixing unit 120 is applied (S1). The first mixed solution 15 and the second reagent 12 are mixed, and a centrifugal force that causes the second mixed solution 16 to flow into the second mixing unit 140 is applied (S7). In a state where the specimen is stored in the second surplus part 118, the second surplus part 118 is moved on the optical path to obtain specimen information (S15). In a state where the first mixed solution 15 is stored in the first mixing unit 120, the first mixing unit 120 is moved on the optical path and the first mixed solution information is acquired (S5). In a state where the second mixed solution 16 is stored in the second mixing unit 140, the second mixing unit 140 is moved on the optical path to acquire the second mixed solution information (S11). Based on the acquired sample information, first mixture information, and second mixture information, the test result of the sample 10 is acquired and displayed (S17).

  Therefore, an appropriate test result can be acquired by the test apparatus 1 based on the specimen information, the first mixed liquid information, and the second mixed liquid information. Further, by rotating the inspection chip 2 around the main shaft 57 and the support shaft 46, not only the first mixed solution 15 and the second mixed solution 16 are generated, but also the second surplus portion 118, the first mixed portion. 120 and the second mixing unit 140 can be optically measured by a common optical path between the one light source 71 and the optical sensor 72. In other words, the specimen 10 in the second surplus portion 118 can be measured with an optical path for measuring the first mixed solution 15 and the second mixed solution 16. Since it is not necessary to provide an optical path for measuring 10 specimens in the second surplus part 118 in the inspection apparatus 1, an appropriate inspection result can be easily obtained. Since a plurality of mixing parts (the first mixing part 120 and the second mixing part 140) can be measured with one optical path, an appropriate inspection result can be obtained more easily.

  From another viewpoint, the inspection chip 2 is given a centrifugal force by being rotated about the main shaft 57 by the inspection device 1, and the direction of the centrifugal force is being changed by being rotated about the support shaft 46. Changed. The liquid circuit 25 of the test chip 2 stores the second surplus part 118 where the separation component 13 of the specimen 10 is stored, the first mixing part 120 where the first mixed liquid 15 is stored, and the second mixed liquid 16. The second mixing unit 140 is provided. In the second surplus portion 118, an optical path is formed on the liquid level of the specimen 10 to be stored or on the lower side thereof by the rotation control of the test chip 2 around the support shaft 46. In the first mixing unit 120, an optical path is formed on the liquid surface of the stored first mixed solution 15 or on the lower side thereof by rotation control around the support shaft 46. In the second mixing unit 140, an optical path is formed on the liquid surface of the stored second mixed liquid 16 or on the lower side of the inspection chip 2 by rotation control around the support shaft 46. Therefore, in the inspection apparatus 1 in which the inspection chip 2 is used, an appropriate inspection result can be easily obtained as described above.

  In the said embodiment, the test | inspection chip 2 is corresponded to the "test object receptacle" of this invention. The plate member 20 corresponds to the “main part” of the present invention. The second surplus part 118 corresponds to the “detection part” of the present invention. The first mixing unit 120 and the second mixing unit 140 correspond to the “mixing unit” of the present invention. The angle changing mechanism 34 corresponds to the “receiver holding means” of the present invention. The main shaft 57 corresponds to the “first shaft” of the present invention, and the support shaft 46 corresponds to the “second shaft” of the present invention. The optical sensor 72 corresponds to the “detector” of the present invention. The CPU 91 that executes step S1 corresponds to the “first centrifugal unit” of the present invention. The CPU 91 that executes steps S1 and S7 corresponds to the “second centrifugal unit” of the present invention. The CPU 91 executing step S13 corresponds to the “first moving unit” of the present invention. The CPU 91 executing step S15 corresponds to the “specimen information acquisition unit” of the present invention. The CPU 91 executing steps S3 and S9 corresponds to the “second moving unit” of the present invention. The CPU 91 executing steps S5 and S11 corresponds to the “mixed liquid information acquisition unit” of the present invention. The CPU 91 executing step S17 corresponds to the “inspection result acquisition unit” of the present invention.

  The first mixing unit 120 corresponds to the “first mixing unit” of the present invention. The second mixing unit 140 corresponds to the “second mixing unit” of the present invention. Step S1 corresponds to the “first centrifugation step” of the present invention. Steps S1 and S7 correspond to the “second centrifugation step” of the present invention. Step S13 corresponds to the “first movement step” of the present invention. Step S15 corresponds to the “specimen information acquisition step” of the present invention. Steps S3 and S9 correspond to the “second movement step” of the present invention. Steps S5 and S11 correspond to the “mixed liquid information acquisition step” of the present invention. Step S17 corresponds to the “inspection result acquisition step” of the present invention.

  The present invention is not limited to the above embodiment, and various modifications can be made. The inspection device 1 and the inspection chip 2 of the above embodiment are merely examples, and the structure, shape, processing, and the like of each can be changed.

  In the above embodiment, the specimen information is acquired from the specimen 10 stored in the second surplus part 118, but the “detection part” of the present invention is not limited to the second surplus part 118. For example, in the test chip 2 of the above embodiment, as described above, the first surplus portion 114 (see FIGS. 5 and 16) can also perform optical measurement using the same optical path. Therefore, sample information may be acquired from the sample 10 stored in the first surplus part 114.

  In the above embodiment, the first mixed liquid information is acquired from the first mixed liquid 15 of the first mixing unit 120 and the second mixed liquid information is acquired from the second mixed liquid 16 of the second mixing unit 140. The “mixing part” of the present invention is not limited to the first mixing part 120 and the second mixing part 140. For example, only one of the first mixing unit 120 and the second mixing unit 140 may be optically measured to acquire only one of the first mixed solution information and the second mixed solution information.

  The test chip 2 of the first modification shown in FIGS. 17 and 18 includes one “mixing unit”. Specifically, the inspection chip 2 according to the first modification includes a mixing unit 160, a first chamber 156, instead of the first mixing unit 120, the seventh passage 136, the second mixing unit 140, and the mixing chamber 155 of the above embodiment. The second chamber 157 and the ninth passage 158 are included in the liquid circuit 25. The mixing unit 160 includes the separated component 13 generated by the first quantification unit 116, the first reagent 11 quantified by the second quantification unit 132, and the second reagent 12 quantified by the third quantification unit 152. It is a part to be mixed, and is a recess that opens upward in a front view.

  The fourth passage 119 and the sixth passage 135 communicate with the mixing unit 160. On the other hand, a first chamber 156 that is a concave portion having a rectangular shape in front view is provided on the right side from a portion where the third supply path 151 and the third fixed amount portion 152 communicate with each other. The first chamber 156 communicates with a second chamber 157 that is a concave portion having a rectangular shape when viewed from the front. The second chamber 157 communicates with the mixing unit 160 via the ninth passage 158. The ninth passage 158 is connected to the upper right end of the second chamber 157 and the upper right end of the mixing unit 160.

  When the test chip 2 of the first modified example is centrifuged as in the above embodiment, the separation component 13 generated by the first quantification unit 116 and the first reagent 11 quantified by the second quantification unit 132 are used. The second reagent 12 quantified by the third quantification unit 152 flows into the mixing unit 160. In the mixing unit 160, the separation component 13, the first reagent 11, and the second reagent 12 are mixed to generate the second mixed solution 16.

  In the test chip 2 of the first modification, the first surplus portion 114, the second surplus portion 118, and the mixing portion 160 are formed at substantially equal distances from the axis O in a front view. When the inspection chip 2 is rotated about the axis O at the measurement position, the optical path is relative to the arcuate locus (dotted arrow in FIG. 18) passing through the first surplus portion 114, the second surplus portion 118, and the mixing portion 160. Moving. That is, the first surplus portion 114, the second surplus portion 118, and the mixing portion 160 can be measured with the same optical path.

  Therefore, the inspection apparatus 1 can acquire liquid mixture information from the second liquid mixture 16 stored in the mixing unit 160 by optical measurement of the mixing unit 160. Moreover, sample information can be acquired from the sample 10 stored in at least one of the first surplus portion 114 and the second surplus portion 118 as in the above embodiment. A test result can be acquired based on the acquired liquid mixture information and specimen information.

  In the test chip 2 of the second modification shown in FIGS. 19 and 20, the separation unit 112 functions as a “detection unit”. Specifically, in the inspection chip 2 of the second modified example, the separation unit 112, the second surplus unit 118, the first mixing unit 120, and the second mixing unit 140 are formed at substantially equal distances from the axis O in a front view. ing. When the inspection chip 2 is rotated around the axis O at the measurement position, an arcuate locus passing through the separation unit 112, the first surplus unit 114, the first mixing unit 120, and the second mixing unit 140 (dotted arrow in FIG. 20). ) To move the optical path relatively. That is, the separation unit 112, the second surplus unit 118, the first mixing unit 120, and the second mixing unit 140 can be measured with the same optical path.

  When the test chip 2 of the first modified example is centrifuged as in the above embodiment, the specimen 10, the first reagent 11, and the second reagent 12 flow in the liquid circuit 25 and are separated as in the above embodiment. The residual component 14 of the specimen 10 is stored in the part 112. Therefore, the test apparatus 1 can acquire sample information from the sample 10 stored in at least one of the separation unit 112 and the second surplus unit 118. Similarly to the above embodiment, the mixture information is obtained from at least one of the first mixture 15 stored in the first mixing unit 120 and the second mixture 16 stored in the second mixing unit 140. Can be obtained. A test result can be acquired based on the acquired liquid mixture information and specimen information.

  The “sample information” is not limited to the hemolytic index of the sample 10 and may be information regarding the sample 10. The “sample information” is not limited to the correction value of the test result, and may be error information indicating that the test apparatus 1 cannot acquire an appropriate test result. For example, the “specimen information” may be information obtained by optical measurement for confirming the presence / absence of liquid or the amount of liquid in the “detection unit”. In this case, the inspection apparatus 1 determines whether or not the sample 10 exists in the “detection unit” based on “sample information” indicating the presence or absence of the liquid or the amount of the liquid, or whether or not the sample 10 is equal to or greater than the required amount. Can be identified. When it is specified that the sample 10 does not exist or does not satisfy the required amount, an “error” may be acquired and notified as a test result.

  The error detection as described above may be performed at an early stage of the inspection procedure (for example, before execution of the centrifugal process). In this case, when “error” is acquired in the error detection, the error notification may be notified as the inspection result, and the process may be terminated without performing the centrifugation process. Since unnecessary centrifugation of the specimen 10 can be omitted, the test result can be acquired quickly. Furthermore, since the inspection chip 2 used in the inspection apparatus 1 only needs to be able to cope with the error detection described above, the developer can design the liquid circuit 25 with high versatility.

  In the above embodiment, the measurement is performed by selectively passing the optical path through the “detection unit” and the “mixing unit” by the rotation control of the inspection chip 2. This part may be switched at least one of rotation and revolution of the inspection chip 2. For example, the measurement target region on the inspection chip 2 may be switched by adjusting both the rotation angle and the revolution angle of the inspection chip 2 or by adjusting only the revolution angle of the inspection chip 2. In these cases, according to the rotation and revolution of the inspection chip 2 or the revolution of the inspection chip 2, a “detection unit” and a “mixing unit” are provided on the trajectory through the optical path with respect to the inspection chip 2. Just do it.

  The “first axis” of the inspection apparatus 1 serving as the revolution center of the inspection chip 2 is not limited to the vertical axis, and may be an axis inclined with respect to the installation surface or a horizontal axis. The “second axis” of the inspection apparatus 1 serving as the center of rotation of the inspection chip 2 is not limited to the horizontal axis, but may be an axis different from the first axis. The rotation angle of the inspection chip 2 is not limited to “0 degrees” to “180 degrees”, but may be other angle ranges (for example, “0 degrees” to “360 degrees”).

  The “specimen” is not limited to blood and may be a liquid. As the “reagent”, various liquid chemicals can be used depending on the specimen to be used. The number of “reagents” injected into the inspection chip 2 is not limited to two (first reagent 11 and second reagent 12), and may be one reagent or three or more reagents.

DESCRIPTION OF SYMBOLS 1 Test | inspection apparatus 2 Test | inspection chip 3 Test | inspection system 10 Sample 11 1st reagent 12 2nd reagent 15 1st liquid mixture 16 2nd liquid mixture 20 Plate material 25 Liquid circuit 34 Angle change mechanism 46 Support axis 57 Main axis 71 Light source 72 Optical sensor 91 CPU
112 Separation unit 114 First surplus unit 118 Second surplus unit 120 First mixing unit 140 Second mixing unit 160 Mixing unit

Claims (8)

A test system including a test target receiver into which a specimen and a reagent that are liquids are injected, and a test apparatus that centrifuges the sample by rotating the test target receiver,
The test object receiver is:
A plate-like main body having a predetermined thickness;
A liquid circuit that extends in a direction perpendicular to the thickness direction in the main body, and that allows the specimen and the reagent to flow;
The liquid circuit is
A detection unit capable of storing the sample and detecting the stored sample;
The liquid mixture of the specimen and the reagent can be stored, and a mixing unit for detecting the stored liquid mixture,
The inspection device includes:
The first axis and the first axis are different from each other in a state where the first axis provided apart from the inspection target receptacle and the liquid circuit extend along the same plane. A receiver holding means for holding it rotatably about two axes;
A light source that emits light;
A detector that forms an optical path with the light source and receives light emitted from the light source;
A first centrifuge that causes the specimen to flow into the detection unit by controlling rotation of the test object receiver held by the receiver holding means about the first axis and the second axis;
The sample and the reagent are mixed by controlling the rotation of the test object receiver held by the receiver holding means about the first axis and the second axis, and A second centrifugal means for flowing into the mixing section;
By controlling rotation around the first axis and the second axis of the test object receiver held by the receiver holding means in a state where at least the specimen is stored in the detection unit, First moving means for moving the detection unit to a position disposed on the optical path;
When the detection unit is arranged on the optical path by the first moving unit, based on the amount of received light detected by the detector, sample information acquisition unit that acquires sample information that is information about the sample;
Controlling rotation of at least one of the first axis and the second axis of the test object receiver held by the receiver holding means in a state where at least the mixed liquid is stored in the mixing unit A second moving means for moving the mixing unit to a position disposed on the optical path;
When the mixing unit is arranged on the optical path by the second moving unit, a mixed liquid information acquisition unit that acquires mixed liquid information that is information about the mixed liquid based on the amount of received light detected by the detector. When,
Test result acquisition means for acquiring a test result of the sample based on the sample information acquired by the sample information acquisition means and the liquid mixture information acquired by the liquid mixture information acquisition means. Inspection system characterized by The mixing unit includes:
A first mixing part for storing a first mixed liquid in which the specimen and the first reagent are mixed;
A second mixing part in which a second mixed liquid in which the first mixed liquid and the second reagent are mixed is stored;
The second centrifuging means mixes the specimen and the first reagent, and after applying a centrifugal force that causes the generated first mixed liquid to flow into the first mixing section, the first mixed liquid and Mixing the second reagent, applying a centrifugal force to flow the generated second mixed liquid into the second mixing unit,
The second moving means is
When the first mixed liquid is stored in the first mixing unit, the test object receiver is moved to a position where the first mixing unit is disposed on the optical path,
When the second mixed liquid is stored in the second mixing unit, the test object receiver is moved to a position where the second mixing unit is disposed on the optical path,
The mixed liquid information acquisition means includes
When the first mixing unit is disposed on the optical path by the second moving unit, the liquid mixture information on the first liquid mixture is acquired,
2. The inspection system according to claim 1, wherein when the second moving unit is arranged on the optical path by the second moving unit, the liquid mixture information on the second liquid mixture is acquired. The detection unit is at least one of a separation unit in which the sample is centrifuged, and a surplus unit in which the sample overflowed from the separation unit during centrifugation of the sample is stored,
The first centrifuge means imparts a centrifugal force that causes the sample to flow into the separation unit and centrifuge,
3. The examination system according to claim 1, wherein the second centrifuging unit applies a centrifugal force that causes the specimen centrifuged at the separation unit to flow into the mixing unit. The first moving unit moves the detection unit to a position on the optical path when the mixed liquid is stored in the mixing unit,
The said 2nd moving means moves the said mixing part to the position arrange | positioned on the said optical path, when the said sample information is acquired by the said sample information acquisition means, The any one of Claim 1 to 3 characterized by the above-mentioned. The inspection system described in Crab. The optical path extends in a direction intersecting with the direction of the centrifugal force applied to the test object receiver held by the receiver holding means,
The first moving means moves the detection unit to a position where the optical path is formed on the liquid level of the specimen stored in the detection unit or on the lower side thereof,
The said 2nd moving means moves the said mixing part to the position where the said optical path is formed in the liquid level of the said liquid mixture stored in the said mixing part, or the lower side of it. Inspection system in any one of. A liquid specimen and reagent are injected, and a centrifugal force is applied by being rotated about a predetermined first axis by a testing device, and is rotated about a second axis different from the first axis. The test object receptacle in which the direction of the centrifugal force is changed,
A plate-like main body extending along the direction of the centrifugal force and having a predetermined thickness;
A liquid circuit that extends in a direction perpendicular to the thickness direction in the main body portion and is capable of flowing the liquid;
The liquid circuit is
A detection unit capable of storing the sample and detecting the stored sample;
The liquid mixture of the specimen and the reagent can be stored, and a mixing unit for detecting the stored liquid mixture,
When the test object receiver is rotated to a first angle around the second axis, the detection unit detects the centrifugal force on the liquid surface of the specimen stored in the detection unit or on the lower side thereof. An optical path extending in a direction intersecting with the direction of
The mixing unit is configured such that, when the inspection object receiver is rotated to a second angle about the second axis, the optical path is on the liquid surface of the mixed solution stored in the mixing unit or on the lower side thereof. A test object receiver characterized in that is formed.   The inspection object receiver according to claim 6, wherein the detection unit and the mixing unit are provided at positions spaced apart from each other by an equal distance from the second axis. A test method executed in a test system including a test target receiver into which a sample and a reagent that are liquids are injected, and a test apparatus that centrifuges the sample by rotating the test target receiver,
The test object receiver is:
A plate-like main body having a predetermined thickness;
A liquid circuit that extends in a direction perpendicular to the thickness direction in the main body, and that allows the specimen and the reagent to flow;
The liquid circuit is
A detection unit capable of storing the sample and detecting the stored sample;
The liquid mixture of the specimen and the reagent can be stored, and a mixing unit for detecting the stored liquid mixture,
The inspection device includes:
The first axis and the first axis are different from each other in a state where the first axis provided apart from the inspection target receptacle and the liquid circuit extend along the same plane. A receiver holding means for holding it rotatably about two axes;
A light source that emits light;
A detector that receives the light emitted from the light source by forming an optical path with the light source;
The inspection method is:
A first centrifugation step for allowing the specimen to flow into the detection unit by controlling rotation of the test subject receptacle held by the receptacle holding means about the first axis and the second axis;
The sample and the reagent are mixed by controlling the rotation of the test object receiver held by the receiver holding means about the first axis and the second axis, and A second centrifugation step for flowing into the mixing section;
By controlling rotation around the first axis and the second axis of the test object receiver held by the receiver holding means in a state where at least the specimen is stored in the detection unit, A first movement step of moving the detection unit to a position disposed on the optical path;
When the detection unit is arranged on the optical path by the first movement step, based on the amount of received light detected by the detector, sample information acquisition step of acquiring sample information that is information about the sample;
Controlling rotation of at least one of the first axis and the second axis of the test object receiver held by the receiver holding means in a state where at least the mixed liquid is stored in the mixing unit A second moving step of moving the mixing unit to a position disposed on the optical path;
When the mixing unit is disposed on the optical path by the second moving step, a mixed liquid information acquisition step of acquiring mixed liquid information that is information regarding the mixed liquid based on the amount of received light detected by the detector. When,
A test result acquisition step of acquiring a test result of the sample based on the sample information acquired by the sample information acquisition step and the liquid mixture information acquired by the liquid mixture information acquisition step. Inspection method characterized by

Images