JP2014045725A - Microorganism detection system and microorganism detection method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a highly reliable microorganism detection system.SOLUTION: A microorganism detection system comprises: an optical microorganism detection device 10 which sucks gas, irradiates the gas with light to detect microorganisms contained in the gas; a chamber 20 capable of storing medium of the microorganisms irradiated with light by the optical microorganism detection device 10; and a pipe 19 that connects an outlet, which is provided for the optical microorganism detection device 10 and from which the gas irradiated with light is discharged, with an inlet provided for the chamber 20. The microorganism detection system may further comprise a temperature control device that controls temperature in the chamber 20. Further, the microorganism detection system may further comprise a humidity control device that controls humidity in the chamber 20.

Description

  The present invention relates to an environmental evaluation technique, and more particularly to a microorganism detection system and a microorganism detection method.

  In a clean room such as a bio clean room, particles such as scattered microorganisms are detected and recorded using a particle detector (see, for example, Patent Document 1 and Non-Patent Document 1). From the particle detection result, it is possible to grasp the deterioration of the air conditioner in the clean room. In addition, a detection record of particles in the clean room may be attached to a product manufactured in the clean room as a reference material. The optical particle detection device, for example, sucks a gas in a clean room and irradiates the sucked gas with light. If the gas contains particles, light is scattered by the particles, so that the number and size of the particles contained in the gas can be detected.

JP 2011-83214 A

Hasegawa, M. et al., “Real-time microorganism detection technology in the air and its application”, Yamatake Corporation, azbil Technical Review December 2009, p.2-7, 2009

  An object of the present invention is to provide a highly reliable microorganism detection system and microorganism detection method.

  According to an aspect of the present invention, (a) a microorganism detecting device that sucks gas and irradiates the gas with light to detect microorganisms contained in the gas; and (b) a microorganism that has been irradiated with light by the microorganism detecting device. A chamber that can store the culture medium, and (c) a pipe that connects the discharge port provided in the microorganism detection apparatus from which the gas irradiated with light is discharged and the injection port provided in the chamber. A microbial detection system is provided.

  Moreover, according to the aspect of the present invention, (a) a gas is sucked into the microorganism detection device, and the microorganisms contained in the gas are detected by irradiating the gas with light; and (b) the microorganism detection via the pipe. A gas irradiated with light is sent from an outlet provided in the apparatus to an inlet provided in the chamber; and (c) a microorganism irradiated with light by a microorganism detecting device on a medium disposed in the chamber. And a method for detecting a microorganism, comprising adsorbing a microorganism.

  According to the present invention, it is possible to provide a highly reliable microorganism detection system and microorganism detection method.

1 is a schematic diagram of a microorganism detection system according to a first embodiment of the present invention. It is a schematic diagram of the optical microorganism detection apparatus which concerns on the 1st Embodiment of this invention. It is sectional drawing of the light source element which concerns on the 1st Embodiment of this invention. It is a schematic diagram of the modification of the optical system of the optical microorganism detection apparatus which concerns on the 1st Embodiment of this invention. It is a schematic diagram of the chamber which concerns on the 1st Embodiment of this invention. It is a schematic diagram of the central processing unit with which the microorganisms detection system which concerns on the 2nd Embodiment of this invention is provided. It is a schematic diagram of the microorganisms detection system which concerns on the 3rd Embodiment of this invention. It is a schematic diagram of the microorganisms detection system which concerns on the 4th Embodiment of this invention. It is a 1st graph which shows the time change of the intensity | strength of the fluorescence which the microorganisms concerning the 5th Embodiment of this invention emit. It is a 1st graph which shows the time change of the intensity | strength of the fluorescence which the microorganisms concerning the 5th Embodiment of this invention emit.

  Embodiments of the present invention will be described below. In the following description of the drawings, the same or similar parts are denoted by the same or similar reference numerals. However, the drawings are schematic. Therefore, specific dimensions and the like should be determined in light of the following description. Moreover, it is a matter of course that portions having different dimensional relationships and ratios are included between the drawings.

(First embodiment)
As shown in FIG. 1, the microorganism detection system according to the first embodiment includes an optical microorganism detection apparatus 10 that sucks gas and irradiates the gas with light to detect microorganisms contained in the gas. A chamber 20 capable of storing a culture medium of microorganisms irradiated with light by the microorganism detection device 10, a discharge port provided in the optical microorganism detection device 10 through which the gas irradiated with light is discharged, and a chamber 20 are provided. And a pipe 19 for connecting the inlet.

  For example, as shown in FIG. 2, the optical microorganism detection apparatus 10 includes a light source element 1 that emits light, a pedestal 2 on which the light source element 1 is mounted, and an irradiation side that collimates the light emitted from the light source element 1. The parallel light lens 11, the irradiation side condensing lens 12 which condenses parallel light, and the injection mechanism 3 which crosses the airflow containing microorganisms into the light condensed by the irradiation side condensing lens 12 are provided. The injection mechanism 3 may include an air valve for changing the flow velocity of the airflow, for example.

  The light source element 1 mounted on the pedestal 2 is disposed on the substrate 101, the anode electrode 102 provided along the surface of the substrate 101, the cathode electrode 103, and the substrate 101, for example, as shown in FIG. A light emitting diode (LED) chip 104. The anode electrode 102 and the LED chip 104 are electrically connected by wire bonding 105. The cathode electrode 103 and the LED chip 104 are electrically connected by wire bonding 106. A reflector 107 is disposed on the substrate 101 so as to surround the LED chip 104. The LED chip 104 is sealed with a transparent resin 108.

  The light emitted from the light source element 1 may be visible light or ultraviolet light. When the light is visible light, the wavelength of the light is, for example, in the range of 400 to 410 nm, for example, 405 nm. When the light is ultraviolet light, the wavelength of the light is in the range of 310 to 380 nm, for example, 355 nm. However, the wavelength of light emitted from the light source element 1 is determined by the type of microorganism to be detected, and is not limited to these numerical values. The pedestal 2 that holds the light source element 1 shown in FIG. 2 is fixed to the housing 31 of the optical microorganism detection apparatus 10.

  The ejection mechanism 3 sucks gas from the outside of the housing 31 with a fan or the like, and jets the sucked gas toward the focal point of the irradiation side condenser lens 12 through a nozzle or the like. For example, the traveling direction of the airflow ejected from the ejection mechanism 3 is set substantially perpendicular to the traveling direction of the light collected by the irradiation side condenser lens 12. Here, when microorganisms are included in the airflow, light hitting the microorganisms is scattered by Mie scattering, and scattered light is generated. In addition, tryptophan, nicotinamide adenine dinucleotide, riboflavin and the like contained in the microorganisms irradiated with light emit fluorescence. The gas is not necessarily ejected toward the focal point of the irradiation side condensing lens 12. For example, the gas may be ejected to a position out of the focus of the irradiation side condensing lens 12 as long as it crosses the light.

  Examples of microorganisms include bacteria. Examples of bacteria include gram negative bacteria, gram positive bacteria, and fungi including mold spores. Examples of gram-negative bacteria include E. coli. Examples of gram positive bacteria include Staphylococcus epidermidis, Bacillus subtilis spores, Micrococcus, and Corynebacterium. Examples of fungi containing mold spores include Aspergillus. The airflow that crosses the light collected by the irradiation side condenser lens 12 is exhausted from the outlet provided in the housing 31 to the pipe 19 shown in FIG.

  The optical microbe detection apparatus 10 includes a detection-side parallel light lens 13 that converts light crossing the airflow ejected by the ejection mechanism 3 into parallel light, and a detection that collects the light that has been collimated by the detection-side parallel light lens 13. A side condensing lens 14. When scattered light is generated by microorganisms contained in the airflow, the scattered light is also converted into parallel light by the detection-side parallel light lens, and then collected by the detection-side condensing lens 14.

  At the focal point of the detection-side condensing lens 14, a scattered light detection unit 16 that detects light scattered by the microorganism is disposed. As the scattered light detection unit 16, a photodiode, a photomultiplier tube, or the like can be used. The number of microorganisms can be measured from the number of times the scattered light detection unit 16 detects the scattered light. In addition, the intensity of scattered light by the microorganism correlates with the particle diameter of the microorganism. Therefore, by detecting the intensity of the scattered light with the scattered light detection unit 16, it is possible to obtain the particle size of the microorganisms scattered in the environment where the optical microorganism detection device 10 is arranged.

  A condensing mirror 15 that is a concave mirror is further arranged in the housing 31 of the optical microorganism detection apparatus 10 in parallel with, for example, an airflow ejected from the ejection mechanism 3. The condensing mirror 15 condenses the fluorescence emitted by the microorganisms contained in the airflow. A fluorescence detection unit 17 that detects fluorescence is disposed at the focal point of the collector mirror 15. When the scattered light detection unit 16 detects the scattered light and the fluorescence detection unit 17 does not detect the fluorescence, it can be seen that the particles included in the airflow are non-living particles. When the scattered light detection unit 16 detects scattered light and the fluorescence detection unit 17 detects fluorescence, it can be seen that the particles contained in the airflow are biological particles such as microorganisms. In addition, the number of microorganisms can be measured from the number of times that the fluorescence detection unit 17 detects fluorescence. For example, a computer that statistically processes the detected light intensity and fluorescence intensity in real time is connected to the scattered light detection unit 16 and the fluorescence detection unit 17.

  The optical system in the optical microorganism detection apparatus 10 is not limited to the example shown in FIG. For example, as shown in FIG. 4, the light that has been converted into parallel light by the parallel light lens 51 may cross the air stream containing microorganisms.

  The gas irradiated with light by the optical microorganism detection apparatus 10 is sent into the chamber 20 through the pipe 19 shown in FIG. As shown in FIG. 5, the pipe 19 may be provided with a valve 21. For example, a petri dish 22 is stored in the chamber 20. A medium 23 is placed in the petri dish 22. At least a part of the microorganisms contained in the gas inspected by the optical microorganism detection apparatus 10 is adsorbed on the medium 23 and cultured on the medium 23. Microorganisms cultured on the medium 23 are appropriately stained and observed with an optical microscope or the like.

  The microorganism detection system according to the first embodiment may further include a temperature control device that controls the temperature in the chamber 20. The temperature control device includes, for example, a temperature adjustment pipe 24 to which a refrigerant is supplied. Alternatively, the temperature control device may include a Peltier element. Most human pathogenic bacteria are mesophilic bacteria and hardly grow below 5 ° C. The minimum growth temperature of microorganisms in food is -11 ° C for bacteria and molds and -10 ° C for yeast. Bacteria die slowly in the frozen state, but can survive for quite a long time. Therefore, the microorganism detection system may include a rapid cooling device that cools the inside of the chamber 20 to a range of −20 ° C. to −10 ° C., which is a temperature at which bacteria are gradually killed but does not grow.

  The microorganism detection system according to the first embodiment may further include a humidity control device that controls the humidity in the chamber 20. The humidity control device includes, for example, a humidity sensor 25 and a dry air flow supply pipe 26. The humidity sensor 25 detects the humidity in the chamber 20. When the humidity value detected by the humidity sensor 25 is higher than a predetermined value, dry air is supplied into the chamber 20 from the dry air flow supply pipe 26, and the humidity in the chamber 20 is controlled. In general, bacteria grow on foods with high water activity, while yeasts grow on foods with relatively low water activity, and molds grow on foods with low water activity. However, halophilic bacteria can grow even with very low water activity, and drought-resistant molds and osmotic-resistant yeast can grow with even lower water activity. Therefore, when microorganisms that can grow even if water activity is low are to be detected, the inside of the chamber 20 may be dehumidified with a humidity control device.

  The gas in the chamber 20 is sucked by the suction device 40 through the pipe 29, the filtering device 30, and the pipe 39 shown in FIG. As shown in FIG. 5, the pipe 29 may be provided with a valve 27. The filtration device 30 shown in FIG. 1 includes a high efficiency particulate air (HEPA) filter, for example, and prevents microorganisms and the like from being discharged into the atmosphere via the suction device 40. As the suction device 40, for example, a pump or the like can be used.

  Conventionally, the gas inspected by the optical microbe detection apparatus has been directly released into the atmosphere, for example. Therefore, there is a problem that even if the microorganism is detected by the optical microorganism detection apparatus, the detected microorganism does not remain as evidence. On the other hand, according to the microorganism detection system according to the first embodiment, the microorganisms contained in the gas inspected by the optical microorganism detection apparatus 10 can be captured and cultured in the medium. Therefore, the microorganisms detected by the optical microorganism detection apparatus 10 can be left as evidence. Therefore, it is possible to improve the reliability of the detection result of the optical microorganism detection apparatus 10.

(Second Embodiment)
Microorganisms may become weaker and die as the exposure dose in the optical microorganism detection apparatus 10 increases. When a certain amount of microorganisms are killed by exposure, the microorganisms do not propagate in the medium 23 shown in FIG. 5, and evidence that living microorganisms are floating in the environment where the optical microorganism detection device 10 is arranged is obtained. I can't. Therefore, it is preferable that the exposure amount of microorganisms in the optical microorganism detection apparatus 10 is set to such an extent that a certain amount of microorganisms irradiated with light can propagate in the medium 23.

  Here, the microorganism detection system according to the second embodiment further includes a central processing unit (CPU) 300 shown in FIG. The CPU 300 is electrically connected to the optical microorganism detection apparatus 10 shown in FIG. An input device 321 and an output device 322 are connected to the CPU 300 shown in FIG. As the input device 321, a keyboard and a mouse can be used. As the output device 322, a display, a printer, a speaker, and the like can be used.

  The CPU 300 includes an exposure condition storage device 301 and an exposure condition setting unit 302. The exposure condition storage device 301 stores, for each type of microorganism, information on exposure conditions that can be propagated in the medium 23 shown in FIG. 5 without killing a certain amount of microorganisms irradiated with light. The information on the exposure conditions is acquired in advance by an experiment or the like and written in the exposure condition storage device 301. When the type of microorganism to be detected is input from the input device 321 illustrated in FIG. 6, the exposure condition setting unit 302 reads the exposure condition corresponding to the input type of microorganism from the exposure condition storage device 301. The exposure condition setting unit 302 sets the exposure conditions of the optical microorganism detection apparatus 10 shown in FIG. 2 or FIG. 4 according to the read exposure conditions.

  For example, the exposure condition setting unit 302 shown in FIG. 6 is an optical type while keeping constant the intensity of light emitted from the light source element 1 shown in FIG. 2 or 4 and the optical system through which the light emitted from the light source element 1 passes. By controlling the injection mechanism 3 of the microorganism detection apparatus 10, the flow rate when the gas containing the microorganisms crosses the light is changed, and the exposure conditions of the microorganisms in the optical microorganism detection apparatus 10 are stored in the exposure condition memory shown in FIG. The exposure conditions read from the apparatus 301 are matched. Here, the exposure time of the microorganism is given by, for example, the beam width of the light irradiated to the microorganism divided by the flow rate of the gas containing the microorganism. The amount is smaller. Moreover, the exposure amount of microorganisms becomes large, so that the flow rate of the gas containing microorganisms becomes slow.

  Alternatively, the exposure condition setting unit 302 shown in FIG. 6 changes the drive current supplied to the light source element 1 shown in FIG. 2 or 4 while maintaining a constant flow rate when the gas containing microorganisms crosses the light. Thus, the intensity of light irradiated to the microorganism is changed, and the exposure condition of the microorganism in the optical microorganism detection apparatus 10 is matched with the exposure condition read from the exposure condition storage device 301 shown in FIG. Here, as the drive current supplied to the light source element 1 increases, the light intensity increases and the exposure amount of microorganisms increases. In addition, as the drive current supplied to the light source element 1 decreases, the light intensity decreases and the exposure amount of microorganisms decreases.

  Alternatively, the exposure condition setting unit 302 shown in FIG. 6 moves the position of the lens constituting the optical system shown in FIG. 2 or 4 while maintaining a constant flow rate when the gas containing microorganisms crosses the light. Thus, the intensity and density of light applied to the microorganism are changed, and the exposure condition of the microorganism in the optical microorganism detection apparatus 10 is matched with the exposure condition read from the exposure condition storage device 301 shown in FIG. The exposure condition setting unit 302 may change the beam width and beam density irradiated to the microorganisms by driving the variable focus lens.

  CPU 300 further includes a detection control unit 303. After the exposure condition setting unit 302 sets the exposure conditions of the optical microorganism detection device 10 shown in FIG. 2, the detection control unit 303 instructs the optical microorganism detection device 10 to start detecting microorganisms. Further, the detection control unit 303 may instruct the optical microorganism detection apparatus 10 to end the detection of microorganisms after a predetermined time has elapsed.

  As described above, according to the microorganism detection system according to the second embodiment, the exposure amount in the optical microorganism detection apparatus 10 is set to such an extent that a certain amount of microorganisms are not killed. Microorganisms propagate in the medium 23. Therefore, it becomes possible to obtain evidence that living microorganisms are floating in the environment where the optical microorganism detection apparatus 10 is arranged.

(Third embodiment)
As shown in FIG. 7, the microorganism detection system according to the third embodiment includes a half mirror 52 that reflects a part of light before the microorganism is irradiated in the optical microorganism detection apparatus 10, and a half mirror 52. And a photodetector 53 for detecting the reflected light. The light intensity detected by the photodetector 53 is sent to the CPU 300. CPU 300 further includes an upper limit light intensity storage device 304 and a feedback control unit 305. The upper limit light intensity storage device 304 stores a predetermined upper limit value of light intensity that does not kill a certain amount of microorganisms. The feedback control unit 305 reads a predetermined upper limit value of the light intensity from the upper limit light intensity storage device 304. Further, the feedback control unit 305 receives the value of the light intensity detected by the photodetector 53.

  Further, the feedback control unit 305 compares the light intensity value detected by the photodetector 53 with a predetermined upper limit value of the light intensity, and the light intensity value detected by the photo detector 53 is the light intensity value. When it is larger than the predetermined upper limit value, the drive current supplied to the light source element 1 is reduced. Optionally, when the light intensity value detected by the photodetector 53 is smaller than a predetermined upper limit value of the light intensity, the drive current supplied to the light source element 1 is increased. When the feedback control unit 305 functions, a warning signal or a warning sound may be emitted from the output device 322. CPU 300 may further include a history storage device 306. The feedback control unit 305 stores the history of the value of the drive current supplied to the light source element 1 in the history storage device 306. By referring to the history of the value of the drive current supplied to the light source element 1, for example, the lifetime of the light source element 1 can be predicted.

(Fourth embodiment)
As shown in FIG. 8, the microorganism detection system according to the fourth embodiment further includes a flow sensor 54 that detects a flow rate of a gas containing microorganisms in the optical microorganism detection apparatus 10. The gas flow velocity detected by the flow sensor 54 is sent to the CPU 300. CPU 300 further includes a lower limit flow rate storage device 307. The lower limit flow rate storage device 307 stores a predetermined lower limit value of the gas flow rate that does not kill a certain amount of microorganisms. The feedback control unit 305 reads a predetermined lower limit value of the gas flow rate from the lower limit flow rate storage device 307. Further, the feedback control unit 305 receives the value of the gas flow velocity detected by the flow sensor 54.

  Further, the feedback control unit 305 compares the value of the gas flow velocity detected by the flow sensor 54 with a predetermined lower limit value of the gas flow velocity, and the value of the gas flow velocity detected by the flow sensor 54 is When it is slower than the predetermined lower limit value of the flow velocity, the valve of the injection mechanism 3 is controlled to increase the gas flow velocity. Optionally, if the value of the gas flow velocity detected by the flow sensor 54 is faster than a predetermined lower limit value of the gas flow velocity, the gas flow velocity is decreased. When the feedback control unit 305 functions, a warning signal or a warning sound may be emitted from the output device 322. The feedback control unit 305 stores the history of controlling the injection mechanism 3 in the history storage device 306. By referring to the control history of the injection mechanism 3, for example, it is possible to grasp the necessity of maintenance of the gas flow path.

(Fifth embodiment)
The exposure conditions that are stored in the exposure condition storage device 301 shown in FIG. 6 and that can be propagated in the medium 23 shown in FIG. The acquisition method of will be described. When a microorganism is irradiated with light, the microorganism emits fluorescence, and as shown in FIG. 9, the intensity of the fluorescence emitted by the microorganism decreases with the passage of light irradiation time. Here, the difference between the intensity of fluorescence at the start of light irradiation on the microorganism and the intensity of fluorescence emitted by the microorganism at an arbitrary time after the start of light irradiation on the microorganism is expressed as “fluorescence intensity”. The amount of attenuation. Further, a value obtained by dividing the attenuation amount of the fluorescence intensity by the fluorescence intensity at the time of starting the irradiation of light to the microorganism is referred to as “fluorescence intensity attenuation rate”. As the attenuation amount of the fluorescence intensity emitted by the microorganism or the attenuation rate of the fluorescence intensity increases, the death rate of the microorganism also increases. Here, the death rate of microorganisms is a value obtained by dividing the number of microorganisms killed by irradiation with light by the total number of microorganisms before irradiation with light.

If the fluorescence emitted by the microorganism falls below the detection limit of the fluorescence detection unit 17 shown in FIG. 2, even if the microorganism subsequently emits fluorescence, the optical microorganism detection device 10 cannot detect the microorganism. Therefore, even if the fluorescence emitted by the microorganisms continues to irradiate the microorganisms after the fluorescence is below the detection limit of the fluorescence detection unit 17, the death rate of the microorganisms only increases. In this case, the time from the start of irradiation of light to the microorganism until the fluorescence emitted by the microorganism falls below the detection limit of the fluorescence detection unit 17 is Δt1 _max, and the constant intensity of light exposed to the microorganism is I _const Then, the maximum exposure amount of the microorganism is set as I _const × Δt1 _max .

Further, the time from the start of irradiation of light to the microorganism until reaching the upper limit of the mortality at which subsequent culture is possible is Δt2 _max, and when Δt2 _max <Δt1 _{max as} shown in FIG. Is set to I _const × Δt2 _max .

  With the method described above, the maximum exposure amount is acquired for each type of microorganism, and the acquired maximum exposure amount or an exposure amount lower than the maximum exposure amount is set as the exposure condition for each type of microorganism. Acquisition of the maximum exposure amount may be performed by the optical microorganism detection apparatus 10 illustrated in FIG. 2 or may be performed by another optical device. For example, the bacteria irradiated with light may be photographed using an imaging element or the like, and the fluorescence intensity attenuation rate may be calculated based on the intensity of each pixel.

(Other embodiments)
As mentioned above, although this invention was described by embodiment, it should not be understood that the description and drawing which form a part of this indication limit this invention. From this disclosure, various alternative embodiments, embodiments, and operation techniques should be apparent to those skilled in the art. For example, in the fifth embodiment, the example in which the maximum exposure amount is obtained with the intensity I _const of light exposed to the microorganism being constant has been described. However, the exposure time of the microorganism is constant, and The maximum exposure amount of the microorganism may be determined by examining the death rate while changing the intensity. Thus, it should be understood that the present invention includes various embodiments and the like not described herein.

DESCRIPTION OF SYMBOLS 1 Light source element 2 Base 3 Injection mechanism 10 Optical microbe detection apparatus 11 Irradiation side parallel light lens 12 Irradiation side condensing lens 13 Detection side parallel light lens 14 Detection side condensing lens 15 Condensing mirror 16 Scattered light detection part 17 Fluorescence detection Parts 19, 29, 39 Pipe 20 Chamber 21 Valve 22 Petroleum 23 Medium 24 Temperature adjustment pipe 25 Humidity sensor 26 Dry air flow supply pipe 27 Valve 30 Filtration device 31 Housing 40 Suction device 51 Parallel light lens 52 Half mirror 53 Photo detector 54 Flow Sensor 101 Substrate 102 Anode electrode 103 Cathode electrode 104 Chip 105 Wire bonding 106 Wire bonding 107 Reflector 108 Transparent resin 301 Exposure condition storage device 302 Exposure condition setting unit 303 Detection control unit 304 Upper limit light intensity storage device 305 Feed bar Control unit 306 history storage device 307 lower limit flow velocity storage device 321 input device 322 output device

Claims (18)

A microorganism detecting device that sucks gas and irradiates the gas with light to detect microorganisms contained in the gas;
A chamber capable of storing a culture medium of the microorganisms irradiated with light by the microorganism detection device;
A pipe that connects an exhaust port provided in the microorganism detection apparatus from which the gas irradiated with the light is exhausted, and an injection port provided in the chamber;
A microorganism detection system comprising:   The microorganism detection system according to claim 1, further comprising an exposure condition storage device that stores an exposure condition of the microorganism in which the microorganism irradiated with the light can propagate in the medium.   The microorganism detection system according to claim 2, wherein an exposure condition of the microorganism in the microorganism detection apparatus is set based on an exposure condition stored in the exposure condition storage device.   The microorganism detection system according to claim 2 or 3, wherein the exposure condition of the microorganism is set based on a relationship between an exposure amount of the microorganism and a death rate of the microorganism.   5. The microorganism detection according to claim 2, wherein the exposure condition of the microorganism is set based on a relationship between an exposure amount of the microorganism and an attenuation rate of the intensity of fluorescence emitted by the microorganism. system.   The microorganism detection system according to any one of claims 2 to 5, wherein the exposure condition of the microorganism includes an intensity of light irradiated to the gas.   The microorganism detection system according to any one of claims 2 to 6, wherein an exposure condition of the microorganism includes a flow rate of the gas across the light.   The microorganism detection system according to any one of claims 1 to 7, further comprising a temperature control device that controls a temperature in the chamber.   The microorganism detection system according to any one of claims 1 to 8, further comprising a humidity control device that controls humidity in the chamber. Aspirating a gas into a microorganism detecting device, irradiating the gas with light to detect microorganisms contained in the gas;
Sending a gas irradiated with the light from a discharge port provided in the microorganism detection device to an injection port provided in the chamber via a pipe;
Adsorbing the microorganisms irradiated with light by the microorganism detection device to a medium disposed in the chamber;
A method for detecting microorganisms, comprising:   The method for detecting a microorganism according to claim 10, further comprising preparing information on exposure conditions of the microorganism capable of propagating the microorganism irradiated with the light in the medium.   The microorganism detection method according to claim 11, further comprising setting an exposure condition for the microorganism in the microorganism detection apparatus based on the information on the prepared exposure condition.   The microorganism detection method according to claim 11 or 12, wherein the microorganism exposure conditions are set based on a relationship between an exposure amount of the microorganism and a kill rate of the microorganism.   The microorganism exposure condition according to any one of claims 11 to 13, wherein the microorganism exposure condition is set based on a relationship between an exposure amount of the microorganism and an attenuation rate of the intensity of fluorescence emitted by the microorganism. Detection method.   The microorganism detection method according to any one of claims 11 to 14, wherein the exposure condition of the microorganism includes the intensity of light irradiated to the gas.   The method for detecting a microorganism according to any one of claims 11 to 15, wherein the exposure condition of the microorganism includes a flow rate of the gas across the light.   The method for detecting a microorganism according to any one of claims 10 to 16, further comprising controlling a temperature in the chamber.   The method for detecting a microorganism according to any one of claims 10 to 17, further comprising controlling humidity in the chamber.