JP2017029075A - Collection detector - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a collection detector capable of compatibly achieving the detection of biological particles in real time and the collection of biological particles in a state capable of being cultivated.SOLUTION: The present invention provides a collection detection apparatus 100 comprising a first collecting mechanism for collecting at least a part of particles in the air used as a sample on a collecting plate 12A provided as a collecting member; and a second collecting mechanism using a collecting plate 12B provided as a carrier to carry and collect at least another part of the particles, which are different from the particles collected on the collecting plate 12A, in the air used as the sample. The collection detector 100 detects bio-based particles based on an increase in amount of the intensity of fluorescence from the particles collected by the collecting plate 12A before and after heating. The second collecting mechanism collects the particles on the collecting plate 12B without being affected by heating on the particles collected on the collecting plate 12A.SELECTED DRAWING: Figure 2

Description

  The present disclosure relates to a collection detection device and a control method, and more particularly, to a collection detection device that detects biological particles based on a change in fluorescence intensity due to heating.

  Conventionally, as a method of detecting particles derived from living organisms floating in the air, a method of collecting and culturing the particles and measuring and observing viable bacteria after the culture has been common. On the other hand, Japanese translations of PCT publication No. 2013-520539 proposes a method of heating the collected particles and detecting biologically derived particles based on the amount of increase in fluorescence intensity from the particles before and after heating. . In the following description, this detection method is also referred to as a heating fluorescence method. Since the heating fluorescence method does not require culturing, measurement results of biological particles can be obtained in real time.

Special table 2013-52039 gazette

  However, since the heated fluorescence method heats the collected particles, the collected particles cannot be cultured. Therefore, it is not possible to identify the type of organism-derived particles (fungi etc.) among the collected particles.

  An object of one aspect of the present disclosure is to provide a collection detection device capable of achieving both detection of biological particles in real time and collection of biological particles in a culturable state.

  According to an embodiment, the collection detection device is a collection detection device for collecting particles in the air and detecting biologically-derived particles from the collected particles. This collection detection device is for collecting a first collection mechanism for collecting at least a part of particles in the air as a sample on a collection member and particles in the air as a sample. A second collecting mechanism for collecting at least some particles different from the particles collected by the member by the carrier, a light receiving element for receiving fluorescence, and a collecting member. A detection mechanism for detecting biologically-derived particles from the particles collected by the collecting member based on the amount of increase in the fluorescence intensity from the collected particles before and after heating; and at least a first capture A control mechanism for controlling the collection operation in the collection mechanism and the detection operation in the detection mechanism. The second collection mechanism is configured to collect particles in the air as a sample without being affected by heating.

  Preferably, the control mechanism is further configured to perform processing for storing, in a memory, information that associates the detection result of the detection mechanism with the carrier.

  More preferably, the associating information includes information indicating a combination of information for specifying the detection result or timing for performing the detection operation by the detection mechanism associated with the detection result and information for specifying the carrier.

  More preferably, identification information is attached to the carrier, and the collection detection device further includes a reading mechanism for reading the identification information. The control mechanism is further configured to control the reading mechanism so that the reading mechanism performs the reading operation at a timing according to the collecting operation in the second collecting mechanism, and the information specifying the carrier is identified. Contains information.

  Preferably, the collection detection device uses one of the plurality of carriers for collecting particles in the second collection mechanism, and uses other than one of the plurality of carriers for collection. A setting mechanism is further provided for setting so as not to exist. The set mechanism performs an operation of setting in association with the detection operation of the detection mechanism.

  More preferably, the setting operation includes an operation of moving one carrier to the collection position in the second collection mechanism.

  Preferably, the plurality of carriers are a plurality of regions provided on one continuous support surface, and the setting operation is other than the region used for collection in the second collection mechanism of the plurality of regions. The operation for covering the area with the cover material is included.

  Preferably, the second collection mechanism uses the collection member separated from the collection member or at least a part of the region separable from the collection member as the carrier.

  Preferably, the second collection mechanism uses an airbag for storing air as a sample as a carrier.

  Preferably, the control mechanism is further configured to cause the second collection mechanism to perform a collection operation when the detection result in the detection mechanism is a predetermined result.

  According to this disclosure, it is possible to achieve both the detection of biological particles in real time and the collection of biological particles in a culturable state using the collection detection device.

It is a graph which shows the change of the fluorescence intensity of the particle | grains derived from a living body, and the change of the fluorescence intensity of dust before and after a heating. It is the schematic of the collection detection apparatus concerning 1st Embodiment. It is a block diagram which shows the specific example of a function structure in a collection detection apparatus. It is a flowchart showing the specific example of the flow of operation | movement of the collection detection apparatus concerning 1st Embodiment. It is a figure showing an example (A) of a memory which memorizes a measurement result, and an example (B) of a memory which memorizes information which associates information which can specify a measurement result, and a collection board. It is the schematic of an example of the mechanism for switching a collection area | region. It is a flowchart showing the specific example of the flow of operation | movement of the collection detection apparatus concerning 2nd Embodiment. It is the schematic of the collection detection apparatus concerning 3rd Embodiment. It is a flowchart showing the specific example of the flow of operation | movement of the collection detection apparatus concerning 3rd Embodiment. It is the schematic of the collection detection apparatus concerning 4th Embodiment. It is a flowchart showing the specific example of the flow of operation | movement of the collection detection apparatus concerning 4th Embodiment.

  Embodiments of the present invention will be described below with reference to the drawings. In the following description, the same parts and components are denoted by the same reference numerals. Their names and functions are also the same. Therefore, these descriptions will not be repeated.

<About the principle of detecting biological particles>
The collection detection device according to the present embodiment is a device for collecting particles floating in the air and detecting biologically derived particles such as pollen, microorganisms, and mold from the collected particles. The principle of detecting organism-derived particles in the collection and detection device in the present embodiment adopts the principle disclosed in JP 2013-520639 A. In this detection, particles floating in the air are collected on the surface of the collecting member, and the collected particles are subjected to a heat treatment, whereby fluorescence from biological particles in the collected particles is detected. Take advantage of increased strength. In detail, the detection method in the collection detection apparatus in this Embodiment is demonstrated using FIG.

  FIG. 1 is a graph showing changes in fluorescence intensity of biological particles and changes in dust fluorescence intensity before and after heating.

  When the biological particles floating in the air are irradiated with ultraviolet light or blue light, the biological particles emit fluorescence. However, in the air, particles that emit fluorescence, such as dust from chemical fibers (hereinafter also referred to as dust), are suspended, and if only fluorescence is detected, it may be from biological particles. It is not distinguished whether it is from.

  On the other hand, as shown in FIG. 1, when heat treatment is performed on biologically derived particles and dust, and the change in fluorescence intensity (fluorescence amount) before and after heating is measured, the fluorescence intensity emitted from the dust is While it does not change, the fluorescence intensity emitted from biological particles increases with heat treatment.

  In the collection and detection device in the present embodiment, the fluorescence intensity before and after heating is measured for particles floating in the air in which the particles derived from living organisms and dust are mixed, and the difference is obtained. Identify the amount of particles from origin.

[First Embodiment]
<Device configuration>
FIG. 2 is a schematic diagram of the collection detection device 100 according to the first embodiment. Referring to FIG. 2, collection / detection device 100 has one cabinet (housing) 3, and collects particles and detects biologically-derived particles therein. The collection detection device 100 includes a control device 200 outside or inside the cabinet 3. The control device 200 includes a signal processing unit 30 for treating a signal representing fluorescence intensity, which will be described later, and a measurement unit 40 for detecting biologically derived particles in the air introduced by measuring the fluorescence intensity. Have. Moreover, the control apparatus 200 controls each part for collection operation | movement and detection operation | movement. In the cabinet 3, a refresh member 13 for refreshing the surface of the collection plate 12A may be arranged. The refresh member 13 includes, for example, a brush formed from a fiber assembly, a wiper, a mechanism for ejecting air toward the surface of the collection plate 12A, and the like. The drive unit of the refresh member 13 (not shown) is also connected to the control device 200, and the refresh operation is also controlled by the control device 200.

  The cabinet 3 is provided with an introduction hole 10 for introducing outside air and a discharge hole 11A for exhausting. The introduction hole 10 may be provided with a filter (prefilter) or a cyclone classifier that removes particles larger than the size of the microorganism to be detected.

A fan 50 as an example of an air introduction mechanism is disposed in the vicinity of the discharge hole 11A. The air introduction mechanism may be a pump or the like. A driving mechanism (not shown) of the fan 50 is controlled by the measuring unit 40, and its rotation is controlled. By rotating the fan 50, as shown in FIG. 2, outside air is introduced into the cabinet 3 through the introduction hole 10 and exhausted through the discharge hole 11A. Preferably, the flow rate of the air introduced by the fan 50 is 0.1 L (liter) / min to 5 m ³ / min.

  The inside of the cabinet 3 is configured to be able to arrange a collection plate 12A as an example of a collection member used as a first collection mechanism for particles floating in the air. The material of the collection plate 12A is not limited to a specific material. The first collection mechanism collects at least some of the particles in the air as a sample on a collection plate 12A that is a collection member.

  Further, the inside of the cabinet 3 is configured to be able to arrange a collection plate 12B as an example of a collection member used as a carrier for carrying in the second collection mechanism of particles floating in the air.

  The material of the collection plate 12B is not limited to a specific material. The material of the collection plate 12B may be the same as or different from the collection plate 12A. For example, the collection plate 12A may be formed of a material having a higher thermal conductivity than the collection plate 12B so as to increase the heating efficiency. Further, the collection plate 12B may have a higher surface friction coefficient than the collection plate 12A so as to prevent the collected particles from separating. Note that the collection plate 12B may be a partial region separable from the collection plate 12A. Furthermore, the collection plate 12B may be a medium for culturing viable bacteria.

  The second collection mechanism collects particles in the air, which are the same as the sample obtained by the first collection mechanism, by carrying them on the collection plate 12B that is a carrier.

  A first collecting mechanism and a second collecting mechanism are provided inside the cabinet 3. Preferably, the first collection mechanism and the second collection mechanism are arranged on the airflow from the introduction hole 10 toward the discharge hole 11A.

  The first collection mechanism and the second collection mechanism collect the particles floating in the air introduced into the cabinet 3 by attaching them to the surfaces of the collection plate 12A and the collection plate 12B, respectively. The collection method in the first collection mechanism and the second collection mechanism is not limited to a specific method. The collection method is, for example, a collection method using an electrostatic force or a collection method using an inertial collision type.

  When the first collection mechanism and the second collection mechanism adopt a collection method using an electrostatic force, each of the first collection mechanism and the second collection mechanism includes, for example, a needle-like discharge electrode. Including. In this case, the collecting plates 12A and 12B have conductivity and are electrically connected to a positive electrode or a ground of a high voltage power source (not shown). A conductive film may be formed on the collecting plates 12A and 12B. The discharge electrode is electrically connected to the negative electrode of the high voltage power source. As a result, a potential difference is generated between the discharge electrode and the surfaces of the collecting plates 12A and 12B, and an upward electric field is formed between them in FIG. The discharge electrode emits electrons due to a potential difference between the surfaces of the collection plates 12A and 12B. As a result, airborne particles introduced from the introduction hole 10 are negatively charged in the vicinity of the discharge electrode and are adsorbed on the surfaces of the collection plates 12A and 12B.

  When the control device 200 rotates the fan 50, the collecting operation is performed. In addition, when the first collection mechanism and the second collection mechanism perform collection using electrostatic force as described above, the control device 200 may apply a high voltage to a discharge electrode (not shown). It becomes.

  A heater 15 is disposed on the surface of the collection plate 12A opposite to the first collection mechanism. A ceramic heater is preferably used as the heater 15. The heater 15 is connected to the control device 200, and the amount of heating (heating time, heating temperature, etc.) is controlled by the control device 200.

  Inside the cabinet 3, a light emitting element 6 and a light receiving unit 9 as a detection mechanism are further provided.

  The light emitting element 6 is preferably a semiconductor laser and irradiates a laser beam. Alternatively, the light emitting element 6 may be an LED (Light Emitting Diode) element. The wavelength may be in the ultraviolet or visible region as long as it excites biological particles and emits fluorescence. Preferably, the wavelength is from 300 nm to 450 nm, which is contained in the microorganism and efficiently excites fluorescent tryptophan, NaDH, riboflavin and the like. The light emitting element 6 irradiates the surface of the collection plate 12A.

  The light receiving unit 9 is a conventionally used photodiode, image sensor, photomultiplier tube, or the like. A photodiode is preferably used. The light receiving unit 9 receives fluorescence from the surface of the collection plate 12A.

  The light emitting element 6 is connected to the control device 200, and light emission and extinction are controlled by the control device 200. The light receiving unit 9 is also connected to the control device 200 and inputs a signal corresponding to the received fluorescence intensity to the control device 200. The control device 200 performs a process for calculating the amount of biological particles based on the signal input from the light receiving unit 9.

  The collection detection device 100 is irradiated with light from the light emitting element 6 from a position (collection position) for collecting particles on the collection plate 12A by the first collection mechanism, and the light receiving unit 9 It further includes a drive unit 2 (FIG. 3) for driving a transport mechanism (not shown) that moves to the position where the fluorescence can be received (detection position) as indicated by the dotted arrow in FIG. The drive part 2 drives the conveyance mechanism for conveying the collection board 12A with a rail, for example, and is controlled by the control apparatus 200. The control device 200 conveys the collection plate 12A to the collection position during the collection operation, and conveys the collection plate 12A to the detection position during the detection operation.

  Preferably, the cabinet 3 is provided with an extraction hole 11B for extracting the collection plate 12B. More preferably, the drive unit 2 (FIG. 3) may further drive a transport mechanism (not shown) that transports the collection plate 12B to the take-out hole 11B as indicated by the one-dot chain line arrow in FIG.

  The heater 15 heats the particles collected on the collection plate 12A by the first collection mechanism, and does not heat the particles collected on the collection plate 12B by the second collection mechanism. Therefore, when biologically-derived particles (fungi) are included in the collected particles, the viable bacteria are collected by the collecting plate 12B, although viable bacteria are not detected after heating by the collecting plate 12A. Preferably, the control device 200 takes out the collecting plate 12B and transports it to the hole 11B before heating the collecting plate 12A with the heater 15. Thereby, taking out of the collection board 12B from the inside of the cabinet 3 can be promoted before heating.

  Preferably, an identifier 12C is added to the collection plate 12B. The identifier 12C is a barcode, a QR code (registered trademark), printing of numbers or the like, physical unevenness such as numbers. In this case, the collection detection device 100 includes a reading device 4 that reads the identifier 12C. The reading device 4 may optically read the identifier 12C according to the type of the identifier 12C, or may physically read the identifier 12C by contact or the like. Preferably, the reading device 4 is arranged at a position where the identifier 12C is read from the collection plate 12B in a state where the collection plate 12B is set at the collection position by the second collection mechanism. The reading device 4 is connected to the control device 200 and inputs the identification information of the collection plate 12B read from the identifier 12C to the control device 200.

<Overview of operation>
The collection detection device 100 detects the biological particles from the collected particles, which are introduced into the cabinet 3 and collects the particles floating in the air as a sample. The detection operation for measuring the quantity is performed. The collection operation is realized by the control device 200 rotating the fan 50. Further, when the first collection mechanism and the second collection mechanism perform collection using electrostatic force as described above, the collection operation is performed by applying a high voltage to the discharge electrode (not shown) by the control device 200. Is realized. The detection operation is realized by the control device 200 causing the heater 15 to generate heat and irradiating the light emitting element 6 with light at a predetermined timing.

  In the collection operation, at least a part of the particles is collected from the air that is the same sample by the first collection mechanism and the second collection mechanism. The particles collected on the surface of the collection plate 12A by the first collection mechanism are the targets for detecting the amount of biological particles. The particles carried on the collection plate 12B by the second collection mechanism can be taken out from the cabinet 3 without being heated. Thereby, for the same sample air, biologically derived particles are detected in real time from the particles collected by the first collection mechanism, and for the identification work by the second collection mechanism. Particles are collected in such a state that can be cultured.

  Preferably, the collection detection device 100 writes in the memory information that associates the detection result of the biological particles from the air that is the same sample with the carrier used for collection by the second collection mechanism. Thereby, correspondence with the detection result of the particle | grains derived from the organism from the air which is the same sample, and the collection result in the 2nd collection mechanism is ensured.

<Functional configuration>
FIG. 3 is a block diagram illustrating a specific example of a functional configuration in the collection detection device 100. Referring to FIG. 3, control device 200 detects signal processing unit 30 for treating a signal representing the fluorescence intensity, and detects living organism-derived particles in the air introduced by measuring the fluorescence intensity. And a measurement unit 40. FIG. 3 shows an example in which the function of the signal processing unit 30 is realized mainly by hardware that is an electric circuit. However, at least a part of these functions may be realized by software, in which the signal processing unit 30 includes a CPU (Central Processing Unit) (not shown) and the CPU executes a predetermined program. . In addition, an example in which the function of the measurement unit 40 is realized by software is shown. However, at least some of these functions may be realized by hardware such as an electric circuit.

  With reference to FIG. 3, the signal processing unit 30 includes a current-voltage conversion circuit 34 connected to the light receiving unit 9 and an amplification circuit 35 connected to the current-voltage conversion circuit 34.

  The measurement unit 40 includes a control unit 41 implemented mainly by a CPU, a writing unit 42 for writing information to the memory 60, and a clock generation unit 43. Further, the measuring unit 40 includes driving units 2A and 2B, a reading device 4, a heater 15, and a driving unit 48 for driving the fan 50.

  Fluorescence from the particles generated when the particles collected on the collection plate 12A are irradiated with excitation light from the light emitting element 6 is collected on the light receiving unit 9. The light receiving unit 9 inputs a signal corresponding to the amount of received light (fluorescence intensity) to the signal processing unit 30. The signal is input to the current-voltage conversion circuit 34.

  The current-voltage conversion circuit 34 detects the peak current value H representing the fluorescence intensity from the signal input from the light receiving unit 9 and converts it to a voltage value Eh. The voltage value Eh is amplified to a preset amplification factor by the amplifier circuit 35 and is output to the measurement unit 40. The control unit 41 of the measurement unit 40 receives an input of the voltage value Eh from the signal processing unit 30. The writing unit 42 sequentially writes the voltage value Eh into the memory 60.

  The clock generator 43 generates a clock signal and outputs it to the controller 41. The control unit 41 controls the drive of the drive unit 48 by outputting a control signal for driving the drive units 2A and 2B, the heater 15, and the fan 50 to the drive unit 48 at a timing based on the clock signal. To do. Moreover, the control part 41 is connected with the light emitting element 6 and the light-receiving part 9, and controls those ON / OFF. The control unit 41 is connected to each of the first collection mechanism and the second collection mechanism, and controls collection operations such as voltage control.

  The control unit 41 includes a calculation unit 411 and a determination unit 412. The calculation unit 411 uses the voltage value Eh stored in the memory 60 to calculate the amount of living organism-derived particles in the introduced air. The writing unit 42 writes the particle amount in the memory 60 as a detection result. Further, the determination unit 412 determines whether or not organism-derived particles are detected to be equal to or greater than the threshold value by comparing a predetermined threshold value with the calculated particle amount. The writing unit 42 may write the determination result in the memory 60 as the detection result.

  Preferably, the writing unit 42 writes, in the memory 60, information that associates the detection result of the biological particles from the air that is the same sample with the carrier used for collection by the second collection mechanism.

  The memory 60 may be a memory (internal memory) mounted on the control device 200, a memory (external memory) mounted on an external device accessible by the control device 200, or a readable / writable medium. Good.

<Operation flow>
FIG. 4 is a flowchart showing a specific example of the operation flow of the collection detection device 100 according to the first embodiment. The operations shown in the flowchart of FIG. 4 are mainly performed by a control unit 41 (CPU) included in the control device 200 reading out and executing a program stored in the memory 60 to exhibit each function of the measurement unit 40. This is realized by the operation of each part of the signal processing unit 30.

  Referring to FIG. 4, control unit 41 causes reading device 4 to read identifier 12C from collection plate 12B set at the collection position by the second collection mechanism (step S1). The identification information of the plate 12B is obtained. The control unit 41 stores the read identification information in the memory 60 (step S3).

  The control part 41 performs the collection operation | movement by a 1st collection mechanism and a 2nd collection mechanism (step S5). That is, in step S5, the control unit 41 drives the fan 50, and further operates the first collection mechanism and the second collection mechanism by applying to the electrode needle (not shown). After the collecting operation, the control unit 41 preferably performs an operation for taking out the collecting plate 12B (step S7). In step S7, for example, the control unit 41 conveys the collection plate 12B to the vicinity of the take-out hole 11B. Preferably, the control unit 41 performs an operation for taking out the collection plate 12B before the heating operation in step S11. Or when performing the operation | movement for taking out the collection board 12B after the heating operation of step S11, Preferably, the control part 41 is the control for the collection board 12B not being influenced by the heating with respect to the collection board 12A. To do. For example, the control unit 41 performs the cooling operation of the collecting plate 12B in parallel during the heating operation in step S11. Alternatively, during step S11, a heat shield member may be provided between the collecting plate 12A and the collecting plate 12B.

  Next, the control unit 41 measures the fluorescence amount F1 on the surface of the collection plate 12A before heating (step S9). That is, in step S9, the control unit 41 illuminates the light emitting element 6 to irradiate the particles collected on the collection plate 12A at the detection position and emits excitation light. Fluorescence from is received for a predetermined time. This fluorescence is emitted from the particles on the surface of the collection plate 12A with the irradiation of excitation light. Based on the current signal from the light receiving unit 9, the measuring unit 40 measures the fluorescence intensity F1 before heating of the particles collected on the collecting plate 12A.

  Next, the control part 41 performs the heating operation | movement with respect to 12 A of collection plates (step S11). That is, in step S11, the control unit 41 energizes the heater 15 for a predetermined time. Thereby, the particles collected on the collection plate 12A are heated.

  Next, the control unit 41 measures the fluorescence amount F2 on the surface of the collection plate 12A after heating (step S13). Prior to step S13, the control unit 41 may perform a cooling operation on the collection plate 12A. In step S13, the control unit 41 illuminates the light emitting element 6 to irradiate the particles collected on the collection plate 12A at the detection position with excitation light and the light receiving unit 9 from the surface of the collection plate 12A. Fluorescence is received for a predetermined time. Based on the current signal from the light receiving unit 9, the control unit 41 measures the fluorescence intensity F2 after heating the particles collected on the collection plate 12A.

  Next, the control unit 41 calculates the amount of biological particles using the amount of increase in fluorescence intensity before and after heating (F2-F1) (step S15). In step S15, for example, the control unit 41 stores in advance a relational expression between the amount of increase in fluorescence intensity before and after heating and the amount of biological particles (concentration, etc.), and the fluorescence intensity obtained from the relational expression. The amount of biological particles can be obtained by substituting the increase amount (F2-F1).

  Preferably, the control unit 41 stores the measurement result in the memory 60 (step S17). At this time, the control unit 41 adds information that can specify the measurement result to the measurement result and stores the information in the memory 60. The information that can specify the measurement result is, for example, a time stamp as information that specifies the specific timing. As another example, the information that can specify the measurement result may be information indicating the number of times of measurement. FIG. 5A is a diagram illustrating an example of a memory 60 that stores measurement results. The memory 60 includes an area for storing a measurement result to which a time stamp as an example of information that can specify the measurement result is added, as shown in FIG.

  More preferably, the control unit 41 stores in the memory 60 information that associates the measurement result with the collection plate 12B used in the collection operation by the second collection mechanism in Step S5. As an example, the control unit 41 adds information (for example, a time stamp) that can specify the measurement result added to the measurement result in step S17 to the identification information of the collection plate 12B stored in the memory 60 in step S3. To do. As another example, the control unit 41 may store the measurement result itself and the identification information of the collection plate 12B in the memory 60 in association with each other. FIG. 5B is a diagram illustrating an example of a memory 60 that stores information that associates information that can specify a measurement result with the collection plate 12B. The memory 60 stores a combination of a time stamp as an example of information that can specify a measurement result as shown in FIG. 5B and an identifier that is an example of identification information of the collection plate 12B. Including the region.

<Effect of the first embodiment>
The collection detection device 100 according to the present embodiment is configured as described above, and the operation is controlled as described above, so that biologically derived particles are detected in real time from particles floating in the air, and Particles in the air, which are the same sample, are collected in a state where they are carried on the carrier without being affected by the heating due to the detection. Therefore, it becomes possible to culture particles floating in the air that is the same sample as the sample used for detection, and to identify the type of biologically-derived particles (fungi etc.) among the collected particles. . Therefore, in the collection and detection apparatus 100 according to the present embodiment, it is possible to realize both the detection of biological particles in real time and the collection of biological particles in a culturable state.

[Second Embodiment]
The collection detection device 100 according to the second embodiment performs a cleaning operation for refreshing the surface of the collection plate 12A using the refresh member 13 after the detection operation. Thereby, in the collection detection device 100, the collection operation and the detection operation by the first collection mechanism using the collection plate 12A can be repeated (continuously).

  In order to cope with repetition of the collection operation and the detection operation by the first collection mechanism, the collection plate 12B used in the collection operation by the second collection mechanism includes a plurality of collection regions. And the collection detection apparatus 100 concerning 2nd Embodiment further contains the mechanism for switching a collection area | region in the collection operation | movement by a 2nd collection mechanism.

  FIG. 6A is a schematic diagram of an example of a mechanism for switching the collection region. With reference to FIG. 6A, the collection detection apparatus 100 according to the second embodiment further includes a cover material 14A for covering the collection plate 12B as an example. The cover member 14A has windows 141, 142,... 1412 corresponding to the plurality of collection regions 121, 122,... 1212 included in the collection plate 12B, and the control unit 41 drives opening and closing of each window (not shown). The opening and closing of each window is controlled by controlling the driving mechanism. That is, the control unit 41 sequentially releases the plurality of windows 141, 142,... 1412 one by one and closes the other windows each time the collection operation is performed by the second collection mechanism. Thus, one particle is carried in the collection regions 121, 122,... 1212 for each collection operation by the second collection mechanism. In this case, preferably, an identifier is added to each of the collection areas 121, 122,... 1212, and the reading device 4 opens the window for each collection operation by the second collection mechanism according to the control of the control unit 41. The identifier of the collection area that has been released, that is, the carrier, is read.

  FIG. 6B is a schematic diagram of another example of a mechanism for switching the collection region. With reference to FIG. 6 (B), the collection detection apparatus 100 concerning 2nd Embodiment further contains the drive part 14B for moving the collection board 12B as another example. In the case of this example, in the collection operation by the second collection mechanism, particles are collected by adhering to a narrow range on the collection plate 12B. The drive part 14B moves the collection board 12B according to control of the control part 41 so that the collection area | regions 121,122, ... 1212 are located one by one in the collection position by a 2nd collection mechanism. As an example, the drive unit 14B rotates the collection plate 12B.

  FIG. 7 is a flowchart showing a specific example of the operation flow of the collection detection device 100 according to the second embodiment. The operation shown in the flowchart of FIG. 7 is also mainly performed by the control unit 41 (CPU) included in the control device 200 reading out and executing a program stored in the memory 60 and performing each function of the measurement unit 40. This is realized by the operation and each part of the signal processing unit 30 operating.

  Referring to FIG. 7, control unit 41 causes reader 4 to read the identifier 12 </ b> C added to the collection area of collection plate 12 </ b> B at the collection position by the second collection mechanism (step S <b> 7). S31), the identification information of the collection area is obtained. The control unit 41 stores the read identification information in the memory 60 (step S33).

  The control part 41 performs the collection operation | movement by a 1st collection mechanism and a 2nd collection mechanism (step S35). Next, the control unit 41 measures the fluorescence amount F1 on the surface of the collection plate 12A before heating (step S37). Next, the control unit 41 performs a heating operation on the collection plate 12A (step S39). Next, the control unit 41 measures the fluorescence amount F2 on the surface of the collection plate 12A after heating (step S41). And the control part 41 calculates the amount of biological particles using the increase amount (F2-F1) of the fluorescence intensity before and behind heating (step S43). The control unit 41 adds a time stamp for specifying the specific timing to the measurement result and stores it in the memory 60 (step S45). Further, the control unit 41 adds the time stamp added to the measurement result in step S43 to the collection area identification information stored in the memory 60 in step S33 (step S47). The operation so far is the same as the operation of the collection detection device 100 according to the first embodiment.

  In the collection detection device 100 according to the second embodiment, when the collection operation and the detection operation are continuously performed (YES in Step S49), the control unit 41 is an operation for switching the collection region of the collection plate 12B. Is performed (step S51). In step S51, for example, the control unit 41 closes the window corresponding to the collection area used for the collection and releases the window corresponding to the next collection area. Further, for example, the control unit 41 moves the collection plate 12B so as to switch the collection area located at the collection position of the second collection operation from the collection area used for the collection to the next collection area. Let Moreover, the control part 41 performs the cleaning operation | movement for refreshing the collection board 12A surface (step S53).

  And the control part 41 repeats operation | movement after step S31 after the operation | movement of said step S51, S53.

<Effects of Second Embodiment>
The collection and detection apparatus 100 according to the present embodiment is configured as described above, and the operation is controlled as described above, whereby particles can be collected and detected continuously. Furthermore, since the correspondence relationship between the measurement result and the carrier carrying the particles contained in the same sample as the sample used for the detection operation is stored in the memory 60, a plurality of detection operations can be performed continuously. Even if it is carried out, it is possible to match the measurement results with the particles used for the culture without confusion.

[Third Embodiment]
In the collection detection device 100 according to the first embodiment and the second embodiment, the collection operation by the second collection mechanism is performed simultaneously with the collection operation by the first collection mechanism. However, the presence or absence of the viable bacteria identification process may be determined according to the measurement result. Therefore, the collection operation by the second collection mechanism may be unnecessary.

  Therefore, the collection detection device 100 according to the third embodiment performs a collection operation by the second collection mechanism based on the measurement result.

  FIG. 8 is a schematic diagram of the collection detection device 100 according to the third embodiment. As shown in FIG. 8, preferably, the collection detection device 100 according to the third embodiment includes a chamber 101 and a first one for performing collection operations by the first collection mechanism, which are independent of each other. And a chamber 102 for performing a collecting operation by two collecting mechanisms. Therefore, the collection detection apparatus 100 according to the third embodiment includes a fan 50A for introducing air as a sample into the chamber 101, and a fan 50B for introducing air as a sample into the chamber 102. The control device 200 drives each of the fans 50A and 50B in accordance with the timing of both detection operations.

  FIG. 9 is a flowchart showing a specific example of the operation flow of the collection detection device 100 according to the third embodiment. In the operation shown in the flowchart of FIG. 9, the control unit 41 (CPU) included in the control device 200 also reads and executes a program stored in the memory 60, and performs each function of the measurement unit 40. This is realized by the operation and each part of the signal processing unit 30 operating.

  With reference to FIG. 9, first, the control unit 41 executes a collecting operation by the first collecting mechanism (step S <b> 71). Next, the control unit 41 measures the fluorescence amount F1 on the surface of the collection plate 12A before heating (step S73). Next, the control part 41 performs the heating operation | movement with respect to 12 A of collection plates (step S75). Next, the control unit 41 measures the fluorescence amount F2 on the surface of the collection plate 12A after heating (step S77). And the control part 41 calculates the amount of particle | grains derived from a living body using the increase amount (F2-F1) of the fluorescence intensity before and behind a heating (step S79). The operations so far are the same as the collection and detection operations by the first collection mechanism in the collection and detection device 100 according to the first embodiment and the collection and detection device 100 according to the second embodiment. is there.

  The control unit 41 determines whether or not the measurement result in step S79 is equal to or greater than a predetermined threshold value (step S81). That is, the control unit 41 determines whether or not organism-derived particles have been detected more than specified from air as a sample. As a result, when the detection result is equal to or greater than the threshold value (YES in step S81), the control unit 41 executes the collection operation by the second collection mechanism (step S83). If the detection result does not reach the threshold value (NO in step S81), the control unit 41 ends the operation without executing the collection operation by the second collection mechanism.

  The collection detection device 100 according to the third embodiment is similar to the operation of the collection detection device 100 according to the first embodiment and the collection detection device 100 according to the second embodiment. The operation of storing the detection result in the memory 60 or the operation of reading the identifier of the collection plate 12B and associating it with the detection result may be further performed. Moreover, the collection detection apparatus 100 concerning 3rd Embodiment also continues the collection operation | movement and detection operation by a 1st collection mechanism similarly to the collection detection apparatus 100 concerning 2nd Embodiment. And a mechanism for continuously performing the collecting operation by the second collecting mechanism along with this.

<Effect of the third actual form>
Since the collection detection device 100 according to the present embodiment is configured as described above and the operation is controlled as described above, an unnecessary collection operation by the second collection mechanism can be suppressed. For example, when a culture medium is used as the collection plate 12B as described above, the culture medium to which particles are attached is discarded if the culture is not performed. In such a case, for example, the waste of the medium can be suppressed by performing the collection operation by the second collection mechanism only when the condition for performing the culture is satisfied, for example, the measurement result is equal to or greater than the threshold value. .

[Fourth Embodiment]
The carrier for carrying particles by the collection operation by the second collection mechanism is not limited to a planar member such as the collection plate 12B. The air itself which is the sample used for the collection operation and the detection operation by the first collection mechanism may be collected.

  FIG. 10 is a schematic diagram of the collection detection device 100 according to the fourth embodiment. As shown in FIG. 10, the carrier for carrying particles by the collection operation by the second collection mechanism may be an airbag 12D. In this case, the collection detecting device 100 according to the fourth embodiment is for introducing air as a sample into the airbag 12D by depressurizing the inside of the chamber 102 in which the airbag 12D is arranged. Including the introduction mechanism 50C, the control device 200 drives the introduction mechanism 50C in accordance with the timing of the collection operation by the second collection mechanism. Moreover, the collection detection device 100 according to the fourth embodiment further includes a collection device 12E for collecting air in the airbag 12D arranged in the chamber 102. Preferably, similarly to the third embodiment, the control device 200 controls the collection device 12E so that the air in the airbag 12D is collected by the collection device 12E based on the measurement result. The collection device 12E may include a collection mechanism similar to the collection mechanism included in the collection detection device 100.

  FIG. 11 is a flowchart showing a specific example of the operation flow of the collection detection device 100 according to the fourth embodiment. The operation shown in the flowchart of FIG. 11 is also mainly performed by the control unit 41 (CPU) included in the control device 200 reading and executing a program stored in the memory 60, and performing each function of the measurement unit 40. This is realized by the operation and each part of the signal processing unit 30 operating.

  With reference to FIG. 11, first, the control unit 41 executes a collecting operation by the first collecting mechanism and the second collecting mechanism (step S <b> 91). In the collection detection device 100 according to the fourth embodiment, particles floating in the air as a sample are carried on the airbag 12D by the collection operation of the second collection mechanism. Next, the control unit 41 measures the fluorescence amount F1 on the surface of the collection plate 12A before heating (step S93). Next, the control part 41 performs the heating operation | movement with respect to 12 A of collection plates (step S95). Next, the control unit 41 measures the amount of fluorescence F2 on the surface of the collection plate 12A after heating (step S97). And the control part 41 calculates the amount of biological particles using the increase amount (F2-F1) of the fluorescence intensity before and after heating (step S99). The operations up to here are substantially the same as the collection operation and the detection operation in the collection detection device 100 according to the first embodiment.

  The controller 41 determines whether or not the measurement result in step S99 is equal to or greater than a predetermined threshold value (step S101). That is, the control unit 41 determines whether or not organism-derived particles have been detected more than specified from air as a sample. As a result, when the detection result is equal to or greater than the threshold value (YES in step S101), the control unit 41 collects particles floating in the air in the airbag 12D with the collection device 12E (step S103). ). Thereafter, the control unit 41 exhausts the air in the airbag 12D (step S105). If the detection result does not reach the threshold value (NO in step S81), the control unit 41 does not collect particles floating in the air in the airbag 12D with the collection device 12E, and the airbag. The air in 12D is exhausted (step S105).

  The collection detection device 100 according to the third embodiment is similar to the operation of the collection detection device 100 according to the first embodiment and the collection detection device 100 according to the second embodiment. The operation of storing the detection result in the memory 60 or the operation of reading the identifier of the collection plate 12B and associating it with the detection result may be further performed. Moreover, the collection detection apparatus 100 concerning 3rd Embodiment also continues the collection operation | movement and detection operation by a 1st collection mechanism similarly to the collection detection apparatus 100 concerning 2nd Embodiment. And a mechanism for continuously performing the collecting operation by the second collecting mechanism along with this.

<Effect of the fourth actual form>
The collection and detection apparatus 100 according to the present embodiment is configured as described above, and the operation is controlled as described above, whereby the air containing the particles that are the sample used for detection is once held in the airbag. In addition, particles are collected from the retained air only when necessary. For this reason, when the culture is not performed, the operation of supporting the particles by the support member which is a planar member can be prevented from being performed.

  The embodiment disclosed this time should be considered as illustrative in all points and not restrictive. The scope of the present invention is defined by the terms of the claims, rather than the description above, and is intended to include any modifications within the scope and meaning equivalent to the terms of the claims.

  2A, 2B, 14B, 48 Drive unit, 3 cabinet, 4 reader, 6 light emitting element, 9 light receiving unit, 10 introduction hole, 11A discharge hole, 11B extraction hole, 12A, 12B collection plate, 12C identifier, 12D airbag , 12E collection device, 13 refresh member, 14A cover material, 15 heater, 30 signal processing unit, 34 voltage conversion circuit, 35 amplification circuit, 40 measurement unit, 41 control unit, 42 writing unit, 43 clock generation unit, 50 , 50A, 50B fan, 50C introduction mechanism, 60 memory, 100 collection detection device, 101, 102 chamber, 121, 122 collection area, 141, 142 window, 200 control device, 411 calculation unit, 412 determination unit.

Claims (10)

A collection detection device for collecting particles in the air and detecting biological particles from the collected particles,
A first collection mechanism for collecting at least a part of particles in the air as a sample on a collection member;
A second collection mechanism for collecting the particles in the air as the sample by carrying at least some particles different from the particles collected by the collection member by a carrier;
A light receiving element for receiving fluorescence;
Based on the amount of increase in fluorescence intensity from the particles collected by the collecting member before and after heating the particles, the biological particles are detected from the particles collected by the collecting member. A detection mechanism;
A control mechanism for controlling at least the collection operation in the first collection mechanism and the detection operation in the detection mechanism, and
The second collection mechanism is a collection detection device configured to collect particles in the air as the sample without being affected by the heating.   The collection detection device according to claim 1, wherein the control mechanism is further configured to perform processing for storing, in a memory, information that associates a detection result of the detection mechanism with the carrier.   The association information includes information indicating a combination of information for specifying the detection result or timing for performing a detection operation in the detection mechanism associated with the detection result and information for specifying the carrier. Item 3. The collection detection device according to Item 2. Identification information is attached to the carrier,
A reading mechanism for reading the identification information;
The control mechanism is further configured to control the reading mechanism to cause the reading mechanism to execute a reading operation at a timing according to the collecting operation in the second collecting mechanism,
The collection detection device according to claim 3, wherein the information specifying the carrier includes the identification information. Set so that one of the plurality of carriers is used for collecting particles in the second collection mechanism, and no one of the plurality of carriers is used for collection. A set mechanism for
The collection detection device according to claim 1, wherein the setting mechanism performs the setting operation in association with a detection operation by the detection mechanism.   The collection detection device according to claim 5, wherein the setting operation includes an operation of moving the one carrier to a collection position in the second collection mechanism. The plurality of carriers are a plurality of regions provided on one continuous carrier surface,
The collection detection device according to claim 5, wherein the setting operation includes an operation for covering a region other than the region used for collection in the second collection mechanism among the plurality of regions with a cover material. .   The second collection mechanism uses the collection member separated from the collection member or at least a part of the region separable from the collection member as the carrier. The collection detection apparatus in any one.   The said 2nd collection mechanism is a collection detection apparatus in any one of Claims 1-7 which uses the airbag for storing the air used as the said sample as the said support body.   The control mechanism is further configured to cause the second collection mechanism to perform a collection operation when a detection result in the detection mechanism is a predetermined result. The collection detection apparatus in any one of.

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