WO2022267965A1 - Comprehensive gas testing apparatus - Google Patents

Abstract

The present invention relates to a comprehensive testing apparatus for microorganisms, such as viruses, and gas components in the air. The apparatus comprises: a microorganism testing module, a gas component testing module and a processing control communication module. Microorganisms, such as viruses of a target category, and harmful gas components, such as PM2.5 particles and formaldehyde, in the air can be tested in real time, disinfection and self-cleaning can be performed, and a real-time alarm can be given. The apparatus in the present invention has the advantages of having a small size, high measurement sensitivity and precision, a quick measurement process, a strong environment adaptability, a low cost, etc.

Description

A gas comprehensive detection device

related application

This application claims the priority of the Chinese invention patent application: 202110712481.9, filed on June 25, 2021, entitled "A Comprehensive Gas Detection Device".

technical field

The invention relates to the field of optical detection, in particular to a rapid and high-sensitivity detection of microorganisms such as viruses, and a comprehensive detection device for high-precision composite gas components and concentrations, and particle size and concentration of suspended particles.

Background technique

In the environment of human life, there are various viruses and other microorganisms in the air, such as influenza virus and other microorganisms, SARS virus and other microorganisms, new coronavirus and other microorganisms (COVID-19), etc. The detection and early warning of viruses and other microorganisms in the indoor ambient air is very necessary. The development of novel coronavirus and other microbial pneumonia sweeping the world has entered the post-epidemic era. Normalized monitoring of viruses and other microorganisms in the air in public places, such as airports, high-speed rail stations, conference rooms, etc., is the focus and difficulty of the current new crown epidemic prevention and control .

PCR technology is currently the most common screening method for microorganisms such as the new coronavirus, but it has disadvantages such as limited use environment, long detection time, and low accuracy rate, and cannot be applied to the detection of viruses and other microorganisms in indoor air environments.

In addition, with the development of society, people's attention to air quality and safety is increasing year by year. On the one hand, with the intensification of pollution, various toxic and harmful gases, and particulate matter make the air in the living environment affect people's health; for some special production scenarios, such as mines, chemical plants, construction sites, etc., it is often full of All kinds of toxic gases and dusts pose a serious threat to people's health and safety. Therefore, there is an increasing demand for a comprehensive detection device capable of fast, real-time, and high-precision detection of compound gas component concentration and particle size.

Contents of the invention

In order to overcome the deficiencies of the prior art, the present invention provides a detection device for microorganisms such as viruses in the air with high measurement sensitivity, high precision, fast measurement process and strong environmental adaptability, and it can also simultaneously detect gas components and particulate matter Comprehensive testing.

Specifically, the present invention provides a comprehensive gas detection device, characterized in that the detection device includes a microbial detection module,

The microbial detection module includes a gate enrichment module and a Raman module, and the gate enrichment module includes a charged component and an electrostatic adsorption component,

The Raman module includes a strong electric field component, a laser excitation source and a Raman detection component,

The charging component is used to discharge the target gas to charge it;

The strong electric field component is arranged outside the electrostatic adsorption component for applying an electric field thereto;

The electrostatic adsorption component is arranged downstream of the charging component for adsorbing microorganisms in the charged target gas, and a laser path is reserved in the adsorption component;

The laser excitation source is used to emit laser light into the electrostatic adsorption component along the laser path, the Raman detection component is used to detect the Raman signal emitted from the electrostatic adsorption component, and the detection device is based on the Raman signal for microbial detection.

Preferably, the Raman module further includes an interference filter component, a Rayleigh filter, a laser absorption cell, and an aspherical long-focus filter component, and the interference filter component and the Rayleigh filter are sequentially arranged on the laser excitation In front of the source, it is used to filter the laser light emitted by it. The laser absorption pool is arranged at the outlet of the adsorption component to collect waste light. The aspheric long focal depth filter component is arranged on the Raman detector In front of the component, it is used to filter the outgoing Raman scattered light to filter out background fluorescence and stray light.

Preferably, the Raman module further includes a multi-pass reflective component, the multi-pass reflective component has at least one reflective surface facing the adsorption component, the laser emitted by the laser excitation source faces the multi-pass reflective component incident on the reflective surface, so that it is reflected at least once within the multi-pass reflective assembly.

Preferably, the detection device further includes a gas component detection module, and the gas component detection module 2 is arranged upstream of the microorganism detection module for detecting gas components.

Preferably, the enrichment gating module includes a first air pump, and the first air pump guides the detected gas into the charging assembly.

Preferably, the detection device further includes a disinfecting and cleaning module, the disinfecting and cleaning module includes a second air pump and a temperature-controlled disinfecting component, the air pump is used to clean the adsorption component through airflow, and the temperature-controlled The disinfecting component is used for disinfecting the gate enrichment module.

Preferably, the detection device further includes a signal processing module, and the signal processing module uses a microbial deconstruction model to process the Raman spectrum of the microbial aerosol to identify the microbial category.

Preferably, the electrostatic adsorption component is made of nano-metal material, preferably, nano-metal particle material.

Preferably, the electrostatic adsorption component is composed of nano-gold or nano-silver particles.

Preferably, the gas component detection module includes a multi-wavelength pulse sequence generator 2.1, a first detection unit, a second detection unit and a gas chamber.

Optionally, the gate enrichment module adopts a turbo pump and a piston pump with adjustable flow rate as an air pump, and adopts gold nanoparticle micromaterials and silver nanoparticle micromaterials (these nanomaterials can be metal nanonets or metal nanolayers) One or more combinations of metal nano-micro materials such as various forms) are used as adsorption components. The various components can be combined with each other.

Optionally, the disinfecting and cleaning module uses the adjustable intensity electric field component in the Raman module to disinfect microorganisms such as viruses by adjusting the voltage; the air pump with adjustable flow rate in the gate-enrichment module is used to adjust the air flow Play a cleaning role.

Principle of invention

The invention constructs a detection device that can realize the detection of microorganisms. In a preferred implementation mode, the present invention is a device capable of detecting microorganisms such as viruses in gas as well as various gas components and particles.

First of all, the device of the present invention uses a charging device to recharge microbial aerosol particles such as viruses with a certain charge, and separates charged microbial aerosol particles such as viruses from the air flow under the force of an electrostatic field—that is, charged After micro-viruses and other microbial particles enter the electric field, they are deflected under the action of the electric field force, and thus are adsorbed on the surface of the metal nano-micro material. Other particulate matter such as PM2.5, PM10, etc. have very little charge and are under the action of an electric field. They are blown out of the gating enrichment module under the action of airflow, thereby achieving the gating effect and greatly reducing the fluorescence of the particulate matter. The impact of the effect on the detection. By designing different plate spacing (mm), charging voltage (kV), electrostatic field strength (kV/cm), air flow rate (L/min), it can achieve high efficiency and high survival of different types of microbial aerosol particles such as viruses rate collection.

Then, the device of the present invention applies a strong electric field to the metal nano-micromaterial through a strong electric field component, so that the number of free electrons contained in it increases by at least one order of magnitude, thereby increasing the electric field intensity on the surface of the metal nanostructure by at least 10 times, and adopts side/front Enhance the Raman effect to the multi-pass surface to obtain the Raman spectrum information of microbial aerosols such as viruses, and combine artificial intelligence image analysis and processing technology to complete the detection of microbial aerosols such as viruses.

Specifically, the incident light is irradiated on the metal nano-micromaterial, preferably, for example, on the gold nano-micromaterial, and the electrons in the nanoparticles vibrate under the action of an external electric field. When the frequency of the incident light is equal to the natural vibration frequency of the electron, Local surface plasmon resonance will occur, and a local enhanced electric field stronger than the excited electric field will be formed on the surface of the metal nanostructure. In the present invention, by applying a strong electric field to the metal nanostructure, the free electrons in the metal are greatly increased under the excitation of the strong electric field, so that the electric field intensity on the surface of the metal nanostructure can be an order of magnitude higher than that before the electric field is applied, and the surface-enhanced Raman scattering (SERS ) is proportional to the fourth power of the surface-enhanced electric field. Therefore, the device of the present invention is sufficient to further increase the Raman signal by 10 ⁴ times, that is, the signal-to-noise ratio is increased by 40 dB.

Moreover, in a preferred implementation mode, the present invention performs spectral preprocessing on the collected Raman spectrum, uses S-G convolution to achieve spectral smoothing, uses a multi-stage low-order polynomial convergence algorithm to achieve baseline alignment, and then uses the maximum normalization method to perform Spectral normalization. The obtained spectrum adopts principal component analysis method and SVM support vector machine supervised artificial intelligence pattern recognition method to obtain a deconstruction model, so as to identify different types of microbial aerosols such as viruses.

The device of the present invention completes the killing and cleaning of enrichment chips by adopting temperature field inactivation and high-flow rate airflow scouring technology, and can also achieve the effect of inactivating microorganisms such as viruses by adjusting the intensity of the high-voltage electric field.

Compared with the prior art, the device of the present invention has the beneficial effects of:

1. The present invention provides a rapid and high-sensitivity detection device for microorganisms such as viruses, and preferably, it can also detect the composition and concentration of composite gas and the particle size and concentration of suspended particles with high precision. The device of the invention screens out the particles in the detection sample through the selection process, can greatly reduce the influence of particle fluorescence on the detection, and can increase the service life of the device.

2. The device of the present invention applies a strong electric field to the metal nano-micromaterial through a strong electric field component, so that the number of free electrons on the surface of the metal nano-micromaterial increases by an order of magnitude, that is, the excitation electric field can be enhanced by 10 times, and the side/forward multi-pass surface is adopted Enhanced Raman surface enhanced remultiplication technology increases the Raman signal by 10 ⁴ times, greatly improving the signal-to-noise ratio and detection sensitivity. By combining artificial intelligence image processing technology, a detection rate higher than 95% can be achieved.

3. The present invention completes the sterilization and cleaning of the enrichment chip through the internal use of temperature field inactivation and high-flow airflow scouring technology, which improves the service life of the detection device and reduces the detection cost.

Description of drawings

Fig. 1 is the structural representation of device of the present invention;

2 is a schematic diagram of the principle of the gas component detection device in Example 2 of the present invention;

Fig. 3 is the schematic diagram of the reflection chamber in the air chamber in embodiment 2;

FIG. 4 is a timing signal sent by the multi-wavelength pulse sequence generation module in Embodiment 2. FIG.

.

detailed description

In order to make the purpose, technical solutions and advantages of the inventive device clearer, the technical solutions in the invention will be clearly described below. Obviously, the described embodiments are some embodiments of the inventive device, rather than all embodiments. Based on the embodiments of the invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the invention.

Example 1:

As shown in FIG. 1 , the schematic architecture of the device in this embodiment 1 is introduced. The comprehensive detection device in this embodiment includes a microorganism detection module 1 , a gas component detection module 2 and a processing control communication module 3 . Wherein the microorganism detection module 1 is one of the main cores of the present invention, the gas component detection module can be selected, and the gas to be measured can be first passed into a detection module for detection, and then the gas is passed into another detection module to perform detection.

The microbial detection module includes a gating enrichment module, a Raman module, and a disinfecting and cleaning module. As shown in Figure 1, the gated enrichment module includes an air pump 1.1.1, a charging component 1.1.2 and an electrostatic adsorption component 1.1.3. The gating and enrichment module is used for gating and enriching microbial aerosols such as viruses.

In this embodiment, the charging component is installed in the charging chamber, the cavity is sealed, one end on both sides communicates with the air pump 1.1.1, and the other end communicates with the adsorption chamber of the electrostatic adsorption component 1.1.3. The charging component 1.1.2 has a high-voltage power supply and one or more sets of tungsten wire pairs with a diameter of 40 μm (other sizes can also be used). The discharge distance of the tungsten wires is set to 8mm, and the charging voltage is +5kV. Aerosol charges.

In this embodiment, an electrostatic adsorption component 1.1.3 is arranged in the adsorption chamber, and the main material of the electrostatic adsorption component 1.1.3 is formed by stacking three-dimensional flocculated gold nanomaterials. The electrostatic adsorption component is used to adsorb microorganisms in the charged target gas, and a laser path is reserved in the adsorption component. It should be noted that the reserved laser path mentioned in the present invention includes two meanings. On the one hand, it means that the gold nanomaterials in the electrostatic adsorption component are sparse enough for the laser to pass through, or that the nanomaterials in the electrostatic adsorption component are along the A sheet or planar structure is formed along the laser path, and the laser passes through gaps in the sheet structure and is at least partially irradiated on the nanometer material.

Raman module includes strong electric field component 1.2.1, laser excitation source 1.2.2, interference filter component 1.2.3, Rayleigh filter 1.2.4, multi-pass reflection component 1.2.5, laser absorption pool 1.2.6, non Spherical long focal depth filter component 1.2.7, Raman detection component 1.2.8.

The Raman module applies a strong electric field to the electrostatic adsorption component 1.1.3 through the strong electric field component 1.2.1, which greatly increases the number of free electrons contained in the electrostatic adsorption component 1.1.3, and enhances the Raman effect principle through the side/forward multi-pass surface Use the Raman detection component 1.2.8 to obtain the Raman spectrum information of microbial aerosols such as viruses. The strong electric field component 1.2.1 is arranged on the upper, lower or left and right sides of the electrostatic adsorption component, sandwiching the electrostatic adsorption component in the middle. The laser excitation source 1.2.2 is used to emit laser light into the electrostatic adsorption assembly along the laser path.

Preferably, in order to increase the adsorption effect, on both sides of the adsorption component, in addition to the strong electric field component 1.2.1, a collection electric field component is added (that is, an additional set of electrode plates is provided on the upper and lower surfaces of the adsorption component). Collecting electric field (1.1.3.1) strength E=3.5kV/cm, composed of silver material, the distance h between parallel electrode plates is 20mm, the width b is 10mm, and the length l is 100mm. The flow rate of the turbo air pump is 10L/min. This module enriches microbial aerosols such as viruses, and screens out particulate matter and achieves a gating effect.

The working process of the gate-enrichment module is as follows: firstly, the gas pump 1.1.1 starts to drive the measured gas into the charged component. The tungsten wire is powered by a high-voltage power supply for high-voltage discharge. Microbial aerosol particles such as viruses with a certain charge are recharged by the charging component. The charged air further enters the electrostatic adsorption component and passes through the electrostatic adsorption component (the electrostatic adsorption component uses nano-particle materials, and the gas can pass through it). In the electrostatic adsorption component, due to the externally applied electric field, the more charged microorganisms are deflected under the action of the electric field force, and thus are adsorbed on the surface of the metal nano-micromaterial. Other particles such as PM2.5, PM10, etc. have very little charge, are subjected to little electric field force, and are blown out of the gate enrichment module under the action of airflow.

The charging component recharges the microbial aerosol particles such as viruses with a certain charge, and separates the charged microbial aerosol particles such as viruses from the air flow under the force of an electrostatic field—that is, charged micro-viruses and other microbial particles After entering the electric field, it is deflected under the action of the electric field force, and thus is adsorbed on the surface of the metal nano-micro material. Other particles such as PM2.5, PM10, etc. have very little charge and are under the action of the electric field. They are blown to the outside of the gating enrichment module under the action of the airflow, so as to achieve the gating effect.

At the same time, since the Raman module applies a strong electric field to the electrostatic adsorption component 1.1.3 through the strong electric field component 1.2.1, the number of free electrons contained in it increases by at least one order of magnitude, thereby increasing the electric field intensity on the surface of the metal nanostructure by at least 10 times, when the microorganisms are adsorbed to the electrostatic adsorption component 1.1.3, the laser light emitted by the laser source 1.2.2 of the Raman module is filtered by the interference filter component 1.2.3 and the Rayleigh filter 1.2.4, and finally irradiated to On the microorganisms adsorbed in the adsorption component. In this embodiment, in order to increase the interaction between the laser and the material, it is preferable to arrange a multi-pass reflective component 1.2.5 on the outside of the adsorption component, with its reflection surface facing the adsorption component 1.1.3. Moreover, the incident direction of the laser is set as an oblique incident, so that the laser is reflected by the multi-pass reflection component 1.2.5, turns back several times in the adsorption component 1.1.3, and then exits from the other side.

In this embodiment, the electric field strength of the strong electric field component in the Raman module is E=4.3kV/cm, which is used to apply a strong electric field to the surface of the nanomaterial; the laser excitation source parameters are 50 microwatts, 532nm wavelength, 1.3 micron diameter, With a depth of focus of 1 micron, the power density is 5*10 ^-5 /10 ^-8 = 500 W/cm ² . The device of the present invention can measure the surface-enhanced Raman spectrum of R6G at 10 ^-8 M, and the number of molecules irradiated by the laser is: 6.02*10 ²³ *10 ^-8 *(0.65*10 ^-5 )2*3.14*10 ^{- 5} ≈5 R6G molecules/m ³ , the signal-to-noise ratio is increased by 40dB, and the measurement sensitivity of viruses and other microorganisms is also increased by 4 orders of magnitude; the interference filter component is a 532nm interference filter; the Rayleigh filter is a notch filter, Its absorption center is located at 532nm; the multi-pass reflection component is an optical lens coated with a high-reflection film with a wavelength of 532nm; the laser absorption pool is a blackened aluminum alloy light collector; High-wavelength transparent film; the Raman detection component is a CCD detector; as shown in Figure 1, the components in the Raman module are coaxially placed according to the optical path.

In this embodiment, the laser absorption pool is a laser trash can, made of aluminum alloy, the surface is oxidized and blackened, and placed at the end of the main optical path.

In this embodiment, the disinfecting and cleaning module uses a high-temperature heating rod and a turbo air pump in the gate-enrichment module to disinfect and clean microorganisms such as viruses in the gate-enrichment module.

In this embodiment, the processing and control communication module adopts artificial intelligence graphic analysis and processing technology, including spectral preprocessing, and then uses the principal component analysis method and SVM support vector machine supervised artificial intelligence pattern recognition method to obtain the spectrum obtained to obtain different viruses. and other unique deconstruction models for microorganisms. During the detection, the collected graphics are compared with the structural model library, so that different types of viruses and other microorganisms can be matched and identified.

In another embodiment, the metal nanomaterial can be a plurality of two-dimensional flat metal nanoplates placed in parallel, preferably two-dimensional flat gold nanoplates, and combined with other two-dimensional flat gold nanoplates and The multi-pass reflective components together form a reflective optical path, which can reflect the laser light emitted by the laser excitation source between the two-dimensional flat gold nanoplate and the multi-pass reflective component. It can effectively enhance the excitation electric field intensity and improve the detection sensitivity of the device.

Example 2

In this embodiment, in addition to the above-mentioned microorganism detection device, the comprehensive detection device of the present invention further includes: a high-precision gas component detection device.

As shown in Figure 2, the composite gas component of the present embodiment, the concentration detection device comprises multi-wavelength pulse sequence generation module 301, the first detection unit 302, the second detection unit 303, gas chamber main body 304, gas chamber inlet assembly 305 and Air chamber outlet assembly 306 . The measured gas enters the gas chamber from the gas chamber inlet assembly 305 and flows out from the gas chamber outlet assembly 306 .

In this embodiment, the multi-wavelength pulse sequence generation module 301 is a module composed of a pulse power supply and four QCL laser light source packages with different central wavelengths. The central wavelengths correspond to the fingerprint wavelengths (532nm, 640nm) of PM2.5 and PM10 particles respectively, CO ₂ 's fingerprint wavelength (4.26μm) and formaldehyde's fingerprint wavelength (3.56μm). The timing trigger mode of multiple wavelength lasers is shown in Figure 4. In this embodiment, the multi-wavelength pulse sequence generation module 301 is arranged at the rightmost end of the gas chamber, and is incident into the gas chamber through the gas chamber window.

Continuing to refer to FIG. 2 , the first detection unit 302 is arranged near the inlet of the air chamber, and adopts a photodiode responsive to the fingerprint wavelength (532nm, 640nm) of PM2.5 and PM10 particles. It is used for measurement by light scattering method, and measures the scattered light intensity and forward scattered light intensity of dual-wavelength pulsed light at a specific scattering angle.

The second detection unit 303 is arranged near the outlet of the gas chamber, and adopts a photodiode responsive to the fingerprint wavelength (4.26 μm) of _CO and formaldehyde (3.56 μm), and is used to measure the multi-wavelength pulsed light by infrared spectroscopic absorption method. The light intensity transmitted by the air chamber main module (4).

The main body 304 of the gas chamber can adopt the structure as shown in FIG. 3 . The gas chamber conforms to the characteristics of the White-type reflective gas chamber, and is composed of 4 concave mirrors with the same curvature radius. In order to obtain a longer optical path, it can also detect when the content of CO ₂ and formaldehyde is very small.

Preferably, the inner wall of the air chamber inlet assembly 305 is coated with a dust-proof coating to prolong the service life of the device and increase the detection accuracy. In addition, the structure at the inlet assembly (5) of the gas chamber conforms to the principle of fluid mechanics, so that the gas to be measured can flow smoothly in the device.

Preferably, the gas chamber outlet assembly 306 is equipped with a low-power silent and vibration-free fan, blowing air toward the outside of the gas chamber, so that the sample to be tested is sucked from the inlet of the gas chamber, and can operate with the required pulse sequence timing or continuously. In addition, the fan has a rapid operation mode. When the device is turned on, the fan can be set to the extreme speed operation mode to clean the air chamber inlet assembly 305, the air chamber main body 304 and the air chamber outlet assembly 6 through the extreme speed operation of the fan, prolonging the service life of the device.

Preferably, the signal processing module includes a filter circuit, a differential amplifier circuit and an STM32L031G6U6 single-chip microcomputer chip.

The use process of device in the present embodiment is as follows:

1: The sample (gas to be measured) is sent in from the gas chamber inlet assembly (5) and sent out from the gas chamber outlet assembly (6) at a constant flow rate by the fan.

2: Start the multi-wavelength pulse sequence generation module (1), so that it emits an optical pulse of a wavelength selected according to needs into the test gas chamber in the form of a pulse sequence with a repetition rate of R and a pulse width of τ. Specifically, Sequentially emit the measurement light pulses of the fingerprint wavelengths (532nm, 640nm) of PM2.5 and PM10 particles into the gas chamber at the incident window at the end of the gas chamber inlet assembly, and send the _CO2 fingerprint wavelength (4.26μm) measurement light pulses to the gas chamber Main body, measuring light pulses of formaldehyde fingerprint wavelength (3.56 μm) to the main body of the air chamber (in this embodiment, the emitted light pulses are the light of the above four fingerprint wavelengths, those skilled in the art can increase the number of wavelengths in the sequence, and the single-shot The more gas components to be measured, the lower the comprehensive cost of the device of the present invention, and the higher the comprehensive benefit), the test is carried out. The dissipation time t of a single pulse with wavelength λ in the gas chamber satisfies

3: Use the first detection unit to detect the scattered light signal, use the pulsed light of the fingerprint wavelength (532nm, 640nm) of PM2.5 and PM10 particles as the measurement light, and use the light scattering method to measure the particle size and corresponding concentration of suspended particles (light scattering method measurement and particle size and concentration calculation of scattering particles can be carried out by existing methods). Select the test light required by the light scattering method from the pulse sequence (for better illustration, only 532nm and 640nm here, but more wavelengths can be selected in practical applications), and determine the scattering coefficient of the selected wavelength. If the wavelength of the test light is λ, the intensity of the outgoing light is I ₀ (λ), the length of the test optical path is l, and the forward scattering light intensity I _s|| (λ) is measured, it can be obtained that after passing through the scattering medium with a length of l , the relationship between different scattering coefficients at different wavelengths is:

That is, the homogeneous scattering coefficient equation, λ _a , λ _b are two kinds of measurement wavelengths. According to this formula, under the same scattering medium conditions, two groups of two, different wavelengths of test light are substituted into the formula, and multiple homogeneous scattering coefficient equations are obtained to form a group of homogeneous scattering coefficient equations. This equation set is used to improve the measurement accuracy of subsequent infrared spectroscopy measurements.

In addition, set the wavelength as λ, measure the emitted light intensity as I ₀ (λ), test the optical path length as l, and measure the forward scattered light intensity I _s|| (λ) to obtain the reference scattering coefficient γ(λ), Its value is

By bringing in the light intensity results of multiple wavelengths of test light, multiple reference scattering coefficients can be obtained.

Simultaneously measure the side scattered light intensity I _s⊥ (λ) of multiple wavelengths selected under a specific scattering angle, and use the light scattering method to analyze the particle size and corresponding concentration of suspended particles;

4: Use the second detection unit to detect the infrared light signal, and use the infrared spectrum detection method to measure. In the infrared spectrum detection, there are corresponding test wavelengths λ ₁ , λ ₂ , λ ₃ λ _n for the gas component 1, gas component 2, gas component 3...gas component n to be measured The corresponding molar molecular absorption coefficients are

The corresponding measured concentrations are c ₁ , c ₂ , c ₃ ···c _n , and the test wavelengths λ ₁ , λ ₂ , λ ₃ ···λ _n are only compatible with the corresponding gas components 1, Gas component 3... There is a strong absorption of gas component n, and the absorption of other components is negligible. Assuming that the detection optical path is L, the infrared spectrum measurement equations are obtained

For the present embodiment, CO2 fingerprint wavelength (4.26 μm) pulsed light and formaldehyde fingerprint wavelength (3.56 μm) pulsed light are respectively used as measurement light, and infrared spectroscopy is used for measurement to obtain the infrared spectrum measurement equation, and step 3 The obtained equations are combined to obtain a system of equations;

5: Simultaneously combine (5)(7)(8), substitute the measured input into the equations for fitting, and solve simultaneously; in the process of solving, refer to the scattering coefficient to select the wavelength similar to the measurement light in the infrared spectrum measurement Determine the size of the scatter term with reference to the scatter coefficient.

For molar molecules with large absorption coefficients (for example, the absorption band intensity of CO ₂ gas at 4.26 μm fingerprint wavelength is 95.5*10 ^-18 cm ^-1 ), it can be directly obtained from the infrared spectrum measurement equation that determines the scattering item, and for molar molecules The absorption coefficient is small (for example, CO gas has an absorption band intensity of 9.8*10 ^-18 cm ^-1 at the fingerprint wavelength of 4.67 μm. Although it is not measured in this example for simplicity, in actual use, it is usually necessary to It is measured, therefore, the present invention covers the situation of its measurement), and the infrared spectrum measurement equation group that has determined the size of the scattering term adopts step difference operation to obtain accurate gas component concentration results, and its operational formula is as follows

Simultaneously combine the reference scattering coefficient obtained in the above steps, the homogeneous scattering coefficient equation, and the infrared spectrum measurement equation group to determine the size of the scattering item, and use step difference to obtain accurate gas component concentrations.

Although the principle of the present invention has been described in detail above in conjunction with the preferred embodiments of the present invention, those skilled in the art should understand that the above embodiments are only explanations for the exemplary implementation of the present invention, and are not intended to limit the scope of the present invention. The details in the embodiments do not constitute a limitation to the scope of the present invention. Without departing from the spirit and scope of the present invention, any obvious changes such as equivalent transformations and simple replacements based on the technical solutions of the present invention fall within the scope of the present invention. within the scope of protection.

Claims (10)

1. A gas comprehensive detection device, characterized in that the detection device includes a microorganism detection module (1),

   The microbial detection module (1) includes a gate enrichment module and a Raman module, and the gate enrichment module includes a charged component and an electrostatic adsorption component,

   The Raman module includes a strong electric field component (1.2.1), a laser excitation source (1.2.2) and a Raman detection component (1.2.8),

   The charging component is used to discharge the target gas to charge it;

   The strong electric field component (1.2.1) is arranged outside the electrostatic adsorption component for applying an electric field thereto;

   The electrostatic adsorption component is arranged downstream of the charging component for adsorbing microorganisms in the charged target gas, and a laser path is reserved in the adsorption component;

   The laser excitation source (1.2.2) is used to emit laser light into the electrostatic adsorption component along the laser path, and the Raman detection component (1.2.8) is used to detect Raman signal, the detection device detects microorganisms based on the Raman signal.
2. The gas comprehensive detection device according to claim 1, characterized in that, the Raman module also includes an interference filter assembly (1.2.3), a Rayleigh filter (1.2.4), a laser absorption cell (1.2.6 ) and an aspherical long focal depth filter assembly (1.2.7), the interference filter assembly (1.2.3) and the Rayleigh filter (1.2.4) are sequentially arranged in front of the laser excitation source (1.2.2) , for filtering the laser light emitted by it, the laser absorption pool (1.2.6) is arranged at the outlet of the adsorption assembly for collecting waste light, and the aspheric long focal depth filter assembly (1.2.7 ) is arranged in front of the Raman detection component (1.2.8), and is used to filter the outgoing Raman scattered light to filter out background fluorescence and stray light.
3. The comprehensive gas detection device according to claim 1, wherein the Raman module further comprises a multi-pass reflection component (1.2.5), and the multi-pass reflection component (1.2.5) has At least one reflective surface of the laser excitation source (1.2.2) is incident on the reflective surface of the multi-pass reflective assembly (1.2.5), so that it is on the multi-pass reflective assembly (1.2.5) is reflected at least once.
4. The gas comprehensive detection device according to claim 1, characterized in that, it also includes a gas component detection module (2), and the gas component detection module (2) is arranged upstream of the microorganism detection module for detecting gas Components are tested.
5. The gas comprehensive detection device according to claim 1, characterized in that, the enrichment gating module includes a first air pump (1.1.1), and the first air pump (1.1.1) guides the gas to be detected into the charged components.
6. The gas comprehensive detection device according to claim 1, characterized in that it also includes a disinfecting and cleaning module, and the disinfecting and cleaning module includes a second air pump (1.3.1) and a temperature-controlled disinfecting component (1.3.2), The air pump (1.3.1) is used to clean the adsorption component through airflow, and the temperature-controlled disinfecting component (1.3.2) is used to disinfect the gate-enrichment module.
7. The comprehensive gas detection device according to claim 1, further comprising a signal processing module, the signal processing module uses a microbial deconstruction model to process the Raman spectrum of the microbial aerosol to identify the type of microorganisms.
8. The comprehensive gas detection device according to claim 1, wherein the electrostatic adsorption component is made of nano-metal material, preferably, nano-metal particle material.
9. The comprehensive gas detection device according to claim 1, wherein the electrostatic adsorption component is composed of nano-gold or nano-silver particles.
10. The gas comprehensive detection device according to claim 4, characterized in that, the gas component detection module (2) comprises a multi-wavelength pulse sequence generator (2.1), a first detection unit (2.2), a second detection unit ( 2.4) and the gas chamber (2.3).

Images