JP2014034549A - External skin preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an external skin preparation having an antibacterial effect on ethanol-resistant bacterium which have resistance to low concentration ethanol.SOLUTION: An external skin preparation, which contains less than 50 mass% of ethanol and 5 to 30 mass% in total of one or more kinds of compounds selected from polyhydric alcohols and alkyl ethers of polyhydric alcohols, has an antibacterial effect on ethanol-resistant bacterium which have resistance to low concentration alcohol.

Description

  The present invention relates to a skin external preparation having an antibacterial effect against ethanol-resistant bacteria having resistance to a low concentration of alcohol.

In recent years, bacterial contamination of foods and the like has attracted attention, and alcohol has been frequently used for sterilization. Due to this, the presence of bacteria having resistance to alcohol, particularly Pichia anomala, has attracted attention (Non-Patent Document 1).

However, in the cosmetics / pharmaceutical industry, antibacterial effects on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida, black mold, etc. have been studied. However, bacteria resistant to alcohol, especially Pichia anomala ( Pichia anomala ) There have been no studies on antibacterial properties against bacteria.

Baking thinner odor generation and factory hygiene management, published in November 2010 by Shigezo Naito, Food Development Analysis Center, http://www.mac.or.jp/mail/101101/02.shtml

  The subject of this invention is providing the skin external preparation which has an antimicrobial effect with respect to the ethanol tolerance microbe which has tolerance with respect to low concentration ethanol.

  The present invention is resistant to low-concentration alcohols containing less than 50% by weight of ethanol and 5 to 30% by weight of one or more selected from polyhydric alcohols and alkyl ethers of polyhydric alcohols. An external preparation for skin having an antibacterial effect against ethanol-resistant bacteria having the above.

  The skin external preparation of the present invention has a low concentration by blending less than 50% by mass of ethanol and 5 to 30% by mass of one or more selected from polyhydric alcohols and alkyl ethers of polyhydric alcohols. It exhibits antibacterial effects against ethanol-resistant bacteria that have resistance to other alcohols.

  Hereinafter, modes for carrying out the present invention will be described.

  Ethanol used in the present invention can be used as long as it is ethanol that is usually used as a raw material for external preparation for skin. Ethanol is blended in an amount of less than 50% by weight, preferably less than 20% by weight, more preferably less than 10% by weight, based on the total amount of the external preparation for skin.

  In the present invention, any polyhydric alcohol that is usually used as a raw material for external preparation for skin can be used. Particularly, those having 4 or less hydroxyl groups in the molecule are preferred for obtaining synergistic antibacterial activity. For example, ethylene glycol, diethylene glycol, triethylene glycol, propylene glycol, dipropylene glycol, 1,3-butylene glycol, 3-methyl-1,3-butanediol (isopropylene glycol), 1,2-pentanediol, hexylene Glycol, glycerin, diglycerin and the like are exemplified, and one or more of these are selected and used.

  In addition, as the alkyl ether of the polyhydric alcohol used in the present invention, glycols or alkyl ethers of glycerin can be preferably used, and in particular, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether (ethyl etherosolve), ethylene glycol monobutyl ether. (Butyl cellosolve), ethylene glycol dimethyl ether, diethylene glycol monomethyl ether (methyl carbitol), diethylene glycol monoethyl ether (ethyl carbitol), diethylene glycol monobutyl ether, diethylene glycol dimethyl ether, diethylene glycol diethyl ether, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene Glico Preferred are rumonoisopropyl ether, dipropylene glycol monomethyl ether, dipropylene glycol monoethyl ether, glyceryl monopalmityl ether (chimyl alcohol), glyceryl monostearyl ether (batyl alcohol), glyceryl monooleyl ether (ceralkyl alcohol), etc. 1 type or 2 types or more are selected and used from these.

  In the present invention, one or two kinds selected from 1,3-butylene glycol and 1,2-pentanediol are preferably used from the viewpoint of antibacterial activity as these polyhydric alcohols or alkyl ethers of polyhydric alcohols. .

  1 type (s) or 2 or more types chosen from the polyhydric alcohol and the alkyl ether of a polyhydric alcohol mix | blend 5-30 mass% with respect to skin external preparation whole quantity, Preferably 5-20 mass% is carried out.

  Although it does not specifically limit as a dosage form of the external preparation for skin of this invention, It can provide with various dosage forms, such as a lotion agent, an emulsion, a gel agent, a cream agent, an ointment, a powder agent, a granule. In addition, skin cosmetics such as lotion, milky lotion, cream, beauty essence, packs, foundation cosmetics such as makeup base lotion and makeup base cream, foundations for each dosage form such as emulsion, oily, solid, etc. It can also be provided as makeup cosmetics such as color and cheek color, skin cleansing agents such as cleansing creams, cleansing lotions, cleansing foams, facial soaps and body shampoos. More specifically, it is preferably used for cleansing lotions, facial cleansers, hair shampoos, body shampoos, lotions and lotions, and cleansing lotions, lotions and lotions are particularly preferred.

  The external preparation for skin of the present invention includes, in addition to the above-mentioned components, various components generally used for external preparations for skin, for example, avocado oil, palm oil, peanut oil, rice bran oil within the range not impairing the effects of the present invention. , Jojoba oil, orange luffy oil, macadamia nut oil, squalane, evening primrose oil, sesame oil, sunflower oil, safflower oil, carola oil, carnauba wax, paraffin wax, lanolin, diisostearyl malate, isostearyl alcohol, fluid Oils such as paraffin, moisturizers such as collagen and hyaluronic acid, vitamins A such as vitamin A oil, retinol and retinol acetate, vitamins B2 such as riboflavin and riboflavin butyrate, vitamins B6 such as pyridoxine hydrochloride, L-ascorbic acid , Magnesium L-ascorbyl phosphate, L-ascorbic acid Vitamin C such as thorium, pantothenic acid such as calcium pantothenate, D-pantothenyl alcohol, pantothenyl ethyl ether and acetyl pantothenyl ethyl ether, vitamin D such as ergocalciferol and cholecalciferol, nicotinic acid and nicotinic acid Amido, nicotinic acids such as benzyl nicotinate, vitamin E such as α-tocopherol and tocopherol acetate, vitamins such as vitamin P and biotin, 2-hydroxy-4-methoxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5 -Sulfonic acid, benzophenone derivatives such as 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid sodium, paraaminobenzoic acid derivatives such as paraaminobenzoic acid, ethyl paraaminobenzoate, octyl paradimethylaminobenzoate, paramethoxy Methoxycinnamic acid derivatives such as 2-ethylhexyl cinnamate and glyceryl di-2-methoxycinnamic acid, salicylic acid derivatives such as octyl salicylate and myristyl salicylate, urocanic acid, 4-tert-butyl-4'-methoxydi UV absorbers such as benzoylmethane and 2- (2'-hydroxy-5'-methylphenyl) benzotriazole, natural polysaccharides such as guar gum, locust bean gum, carrageenan, quince seed, pectin and mannan, xanthan gum and dextran , Natural polysaccharides such as curdlan and hyaluronic acid, animal polymers such as gelatin, casein, albumin and collagen, and celluloses such as methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose and carboxymethylcellulose Synthetic polymers, soluble starch, carboxymethyl starch, starch-based semi-synthetic polymers such as methyl starch, alginic acid-based semi-synthetic polymers such as alginic acid propylene glycol ester, alginate, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, polyacrylic Synthetic polymers such as sodium acid and polyethylene oxide, water-soluble polymers such as inorganic polymers such as bentonite, laponite and colloidal alumina, antioxidants such as dibutylhydroxytoluene, butylhydroxyanisole and gallic acid esters, higher fatty acid soaps , Anionic surfactants such as alkyl sulfates, polyoxyethylene alkyl ether sulfates, acylmethyl taurates, alkyl ether phosphates, acyl amino acid salts, chlorides Cationic surfactants such as rualkyltrimethylammonium chloride, dialkyldimethylammonium chloride, benzalkonium chloride, alkyldimethylaminoacetic acid betaines, alkylamidodimethylaminoacetic acid betaines, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaines Amphoteric surfactants such as polyoxyethylene alkyl ether, polyoxyethylene alkylphenyl ether, polyoxyethylene polyoxypropylene glycol, polyoxyethylene polyoxypropylene alkyl ether, polyoxyalkylene fatty acid glycerin ester, polyoxyalkylene sorbitan ester, Sorbit oligomer tetraester, Sorbit oligomer hexaester, polyethylene glycol ester, glycerin fat Nonionic surfactants such as esters, sorbitan fatty acid esters, sucrose fatty acid esters, alkylolamides, fatty acid amides, metal ions such as ethylenediaminetetraacetic acid sodium salt, sodium polyphosphate, citric acid, sodium metaphosphate, succinic acid, gluconic acid Blocking agent, placenta extract, Sahakuhi extract, glutathione, kojic acid and its derivatives, whitening agents such as hydroquinone and its derivatives such as hydroquinone glycoside, glycyrrhizic acid, glycyrrhetinic acid, allantoin, azulene, hydrocortisone, ε-aminocaprone Anti-inflammatory agents such as acids, astringents such as allantoin hydroxyaluminum, aluminum chloride, tannic acid, citric acid and lactic acid, cooling agents such as menthol and camphor, diphenhydramine hydrochloride, chlorphenirami maleate Antihistamines etc., estradiol, estrone, sebum inhibitors, such as ethinyl estradiol, salicylic acid, keratin peeling and dissolution agent, such as resorcin, alpha-hydroxy acids and the like can be blended.

Examples of ethanol-resistant bacteria having resistance to a low concentration of alcohol include fungi that perform alcohol fermentation, fungi that have alcohol utilization, and the like. Among these bacteria Pichia (pichia) yeast belonging to the genus, for particularly Pichia anomala (Pichia anomala) bacteria, to exert the antimicrobial activity.

  EXAMPLES Hereinafter, the present invention will be specifically described with reference to examples, but the scope of the present invention is not limited thereby. The blending amount is mass% unless otherwise specified.

[Evaluation of antibacterial activity]
Pichia anomala , Escherichia coli , Staphylococcus aureus , Pseudomonas aeruginosa , Candida albicans , and black mold ( Aspergillus niger ) Inoculate 10 ⁶ Pichia anomala, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, 10 ⁵ Candida and black mold per gram of sample, and culture at 37 ° C and 25 ° C, respectively. Then, the viable cell count after 2 weeks was measured. Antibacterial activity passed after 2 weeks, when Pichia anomala, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were killed, and Candida and black molds when the viable count was 1/1000 or less It was judged that. In addition, the antibacterial activity test result was shown as “◯” when it passed, and “x” when it failed.

  A lotion was prepared by a conventional method according to the formulation shown in Table 1, and an antibacterial activity test was conducted. As a result, in Comparative Example 1 in which 1,3-butylene glycol was blended only in an amount of 3% by mass below the blending lower limit, the antibacterial effect against Pichia anomalar was not observed, and the tendency was on the contrary. On the other hand, in Example 1 of the present invention, an antibacterial effect was recognized against all bacterial species.

  A cleansing lotion was prepared by a conventional method with the formulation shown in Table 2, and an antibacterial activity test was conducted. As a result, in Comparative Example 2 in which 1,3-butylene glycol was blended only at 4% by mass below the blending lower limit, the antibacterial effect against Pichia anomalar was not observed, and the tendency was on the contrary. On the other hand, in Example 2 of the present invention, an antibacterial effect was recognized against all bacterial species.

  An anti-inflammatory lotion was prepared by a conventional method according to the formulation shown in Table 3, and an antibacterial activity test was conducted. As a result, in Comparative Example 3 in which 1,3-butylene glycol was blended only in an amount of 3% by mass below the blending lower limit, the antibacterial effect against Pichia anomalar was not observed, and the tendency was on the contrary. On the other hand, in Example 3 of the present invention, an antibacterial effect was recognized against all bacterial species.

[Example 4] Moisturizing serum
(1) Purified water Amount that makes the total amount 100
(2) 1,3-butylene glycol 10.0
(3) Glycerin 10.0
(4) Carboxyvinyl polymer (1% by mass) 5.0
(5) Methyl paraoxybenzoate 0.15
(6) Fragrance 0.1
(7) POE hydrogenated castor oil 0.05

[Example 5] Moisturizing serum
(1) Purified water Amount that makes the total amount 100
(2) 1,3-butylene glycol 15.0
(3) Glycerin 15.0
(4) Trimethylglycine 5.0
(5) Phenoxyethanol 0.3
(6) Sodium hyaluronate 5.0
(7) Fragrance 0.1
(8) POE hydrogenated castor oil 0.05

[Example 6] Whitening serum
(1) Purified water Amount that makes the total amount 100
(2) 1,3-butylene glycol 8.0
(3) Glycerin 6.0
(4) Diglycerin 7.0
(5) Ethanol 6.0
(6) Ascorbic acid phosphate magnesium salt 3.0
(7) Phenoxyethanol 0.15
(8) Fragrance 0.1
(9) POE hydrogenated castor oil 0.05

[Example 7] Whitening lotion
(1) Purified water Amount that makes the total amount 100
(2) Arbutin 3.0
(3) Glycerin 10.0
(4) 1,2-pentanediol 2.0
(5) Ethanol 7.0

[Example 8] Emulsion
(1) Purified water Amount that makes the total amount 100
(2) Glycerin 6.0
(3) 1,3-butylene glycol 3.5
(4) 1,2-pentanediol 2.0
(5) Alkyl-modified carboxyvinyl polymer 0.5
(6) Phenoxyethanol 0.3
(7) Methylparaben 0.1
(8) Behenyl alcohol 6.0
(9) Vaseline 3.5
(10) Cetyl isooctanoate 6.0
(11) Dimethylpolysiloxane 3.0
(12) Polyglyceryl myristate 0.5
(13) Stearyl glucoside 0.5

[Example 9] Cosmetic emulsion
(1) Purified water Amount that makes the total amount 100
(2) Glycerin 6.0
(3) 1,3-butylene glycol 6.0
(4) Behenyl alcohol 6.0
(5) Petrolatum 3.5
(6) Cetyl isooctanoate 6.0
(7) Dimethicone 3.0
(8) Decaglyceryl myristate 1.0
(9) Stearyl glucoside 0.5
(10) Alkyl-modified carboxyvinyl polymer
(2.5% by weight aqueous solution) 20.0
(11) Phenoxyethanol 0.3
(12) Paraben 0.1
(13) Allantoin 0.3

[Example 10] Cream
(1) Purified water Amount that makes the total amount 100
(2) Glycerin 10.0
(3) DPG 5.0
(4) Liquid paraffin 8.5
(5) Isopropyl myristate 8.5
(6) Glyceryl laurate 2.0
(7) Stearyl alcohol 2.0
(8) Hydrogenated lanolin fatty acid ester 3.0
(9) POE stearyl ether 2.0
(10) Ethanol 5.0
(11) Phenoxyethanol 0.2

Example 11 Emulsion
(1) Purified water Amount that makes the total amount 100
(2) Glycerin 2.0
(3) 1,3-butylene glycol 17.0
(4) Sodium stearoyl glutamate 0.15
(5) Squalane 2.5
(6) Behenyl alcohol 0.5
(7) Owl 0.3
(8) Glyceryl stearate 0.3
(9) Stearic acid 0.3
(10) Alkyl-modified carboxyvinyl polymer
(1% by weight aqueous solution) 10.0

[Example 12] Face wash
(1) Purified water Amount that makes the total amount 100
(1) Stearic acid 10.0
(2) Palmitic acid 10.0
(3) Myristic acid 12.0
(4) Lauric acid 4.0
(5) Oleyl alcohol 1.5
(6) Lanolin alcohol 1.0
(7) Potassium hydroxide 6.0
(8) Amount with 100% purified water
(9) 1,3-butylene glycol 10.0
(10) Phenoxyethanol 0.3

[Example 13] Body shampoo
(1) Lauric acid 5.0
(2) Myristic acid 7.5
(3) Lauroyl diethanolamide 5.0
(4) 1,3-butylene glycol 10.0
(5) Potassium hydroxide (10% by weight aqueous solution) 3.0
(6) Amount with 100% purified water
(7) Phenoxyethanol 0.3

  As a result of performing the antibacterial activity test about Examples 4-13, it passed with respect to all the microbes.

Claims (1)

  Ethanol resistance having resistance to low-concentration alcohol, containing less than 50% by mass of ethanol and 5 to 30% by mass of one or more selected from polyhydric alcohols and alkyl ethers of polyhydric alcohols A topical skin preparation that has antibacterial effects against bacteria.