JP2013545726A - 実験・検査用消耗品の表面上に存在する残留核酸の処理方法 - Google Patents
実験・検査用消耗品の表面上に存在する残留核酸の処理方法 Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
- A61L2/186—Peroxide solutions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/20—Gaseous substances, e.g. vapours
- A61L2/206—Ethylene oxide
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/20—Gaseous substances, e.g. vapours
- A61L2/208—Hydrogen peroxide
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L12/00—Methods or apparatus for disinfecting or sterilising contact lenses; Accessories therefor
- A61L12/08—Methods or apparatus for disinfecting or sterilising contact lenses; Accessories therefor using chemical substances
- A61L12/12—Non-macromolecular oxygen-containing compounds, e.g. hydrogen peroxide or ozone
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/02—Membrane cleaning or sterilisation ; Membrane regeneration
- B01D65/06—Membrane cleaning or sterilisation ; Membrane regeneration with special washing compositions
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Abstract
Description
核酸増幅反応は、反応媒体中に最初に存在する核酸配列からの新たな核酸分子の合成を可能にするポリメラーゼの作用を含む一連の酵素反応からなる。
i)消耗品の表面を気相のエチレンオキシドで処理し、ついで
ii)液相または気相の過酸化水素で表面を処理することを含む。
1.5ml ポリプロピレンチューブからなる幾つかの消耗品を既知量のDNA、RNAまたは微生物で並行して汚染した。ついで、これらの消耗品をガンマ線で滅菌し(0〜60kGyの線量)、または気相のエチレンオキシドで処理した。滅菌後、残留核酸の存在をPCR(DNAの場合)およびTMA(RNAの場合)により判定し、滅菌されていない対照消耗品と比較する。
プロトコール1で使用したのと同じタイプの幾つかの消耗品を同じ既知量のDNA、RNAまたは微生物で汚染した。ついで、これらの消耗品を、液体溶液中の過酸化水素(それぞれ、3%および30% H2O2)での処理により処理した。該方法の終了時に、60℃の温度での真空下の乾燥の工程により、該過酸化水素を蒸発させる。処理後、残留核酸の存在をPCR(DNAの場合)およびTMA(RNAの場合)により判定し、未処理サンプルと比較した。
プロトコール1で使用したのと同じタイプの幾つかの消耗品を同じ既知量のDNA、RNAまたは微生物で汚染した。ついで、それらの消耗品の一部を、本発明に従い、気体エチレンオキシドで、ついで3%または30% 過酸化水素で処理した。他の一部を、気体エチレンオキシドの代わりにガンマ線で処理し、ついで過酸化水素で処理した。どちらの場合も、60℃の温度での真空乾燥工程により過酸化水素を蒸発させた。処理後、残留核酸の存在をPCR(DNAの場合)およびTMA(RNAの場合)により判定し、未処理サンプルと比較した。
幾つかのフィルターを含む幾つかの複雑な消耗品を同じ既知量の微生物(ジー・ステアロテルモフィルス(G.stearothermophilus))で汚染した。ついで、それらの消耗品の一部を、本発明の方法に従い、気体エチレンオキシド、ついで約90%の気体過酸化水素で処理した。対照として用いた該消耗品の他の一部は、処理しなかった。残留核酸(DNA)の存在をPCRにより判定した。図4は、種々のPCRサイクル中に増幅されたDNAの量を示す。該処理サンプルにおける増幅の非存在が認められうる。
Claims (15)
- 消耗品の表面上に存在する増幅可能な残留核酸の処理方法であって、以下の工程:
i)消耗品の表面を気相のエチレンオキシドで処理し、ついで
ii)液相または気相の過酸化水素で表面を処理することを特徴とする方法。 - 工程i)におけるエチレンオキシドを70%〜90%(v/v)の濃度で使用する、請求項1記載の方法。
- 工程ii)における過酸化水素が、25%〜60%(v/v)の濃度、好ましくは30%(v/v)を超える濃度の液相である、請求項1または2記載の方法。
- 液相の過酸化水素を50℃〜70℃の温度、好ましくは60℃を超える温度に加熱する、請求項3記載の方法。
- 液相の過酸化水素が、工程ii)において、汚染除去すべき表面と少なくとも40分間にわたって接触している、請求項2または3記載の方法。
- 過酸化水素での処理を気相で行う、請求項1記載の方法。
- 過酸化水素を気化させ、ついで30%を超える、好ましくは50%を超える、より好ましくは80%を超える濃度にする、請求項6記載の方法。
- 過酸化水素を、60℃未満の温度で電場を適用することによりプラズマ状態にする、請求項7記載の方法。
- 工程i)と工程ii)との間に蒸発工程を更に含む、請求項1〜8のいずれか1項に記載の方法。
- 分子生物学における消耗品が、部分的に不織性の包装内に予め包装されている、請求項1〜9のいずれか1項に記載の方法。
- 工程i)およびii)の1つの前に真空を適用する、請求項1〜10のいずれか1項に記載の方法。
- 工程i)と工程ii)との間に残留エチレンオキシドを除去する、請求項1〜11のいずれか1項に記載の方法。
- 工程ii)の後で消耗品の表面から残留過酸化水素を除去する、請求項1〜12のいずれか1項に記載の方法。
- 増幅可能な残留核酸を含有しない、請求項1〜13のいずれか1項に記載の方法により処理された消耗品。
- 消耗品がフィルターまたは膜を含み、該フィルターまたは膜が、好ましくは、ポリプロピレン、PESまたはPVDFのフィルターまたは膜である、請求項14記載の消耗品。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1058516 | 2010-10-19 | ||
FR1058516A FR2966056B1 (fr) | 2010-10-19 | 2010-10-19 | Procede de traitement des acides nucleiques residuels presents a la surface des consommables de laboratoire |
PCT/IB2011/054619 WO2012052913A1 (en) | 2010-10-19 | 2011-10-18 | Process for treatment of residual nucleic acids present on the surface of laboratory consumables |
Publications (2)
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JP2013545726A true JP2013545726A (ja) | 2013-12-26 |
JP5758006B2 JP5758006B2 (ja) | 2015-08-05 |
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JP2013534431A Active JP5758006B2 (ja) | 2010-10-19 | 2011-10-18 | 実験・検査用消耗品の表面上に存在する残留核酸の処理方法 |
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US (1) | US8765054B2 (ja) |
EP (1) | EP2629806B1 (ja) |
JP (1) | JP5758006B2 (ja) |
CN (1) | CN103096937B (ja) |
ES (1) | ES2553975T3 (ja) |
FR (1) | FR2966056B1 (ja) |
WO (1) | WO2012052913A1 (ja) |
Families Citing this family (3)
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US9804069B2 (en) * | 2012-12-19 | 2017-10-31 | Dnae Group Holdings Limited | Methods for degrading nucleic acid |
WO2015175564A1 (en) | 2014-05-13 | 2015-11-19 | Rasirc, Inc. | Methods and systems for delivering process gases to critical process applications |
CN112808690B (zh) * | 2021-01-21 | 2022-06-28 | 人和未来生物科技(长沙)有限公司 | 一种消除核酸残留的方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2003006650A1 (fr) * | 2001-07-09 | 2003-01-23 | Asahi Kasei Kabushiki Kaisha | Procede de purification d'acide nucleique au moyen d'un non-tisse et procede de detection |
WO2005007884A1 (en) * | 2003-07-15 | 2005-01-27 | The Secretary Of State For The Home Department | Use of ethilene oxide to provide dna dree items |
EP1792631A1 (en) * | 2005-11-30 | 2007-06-06 | Roche Diagnostics GmbH | Process for DNA decontamination |
JP2009511016A (ja) * | 2005-10-07 | 2009-03-19 | ベクトン・ディキンソン・アンド・カンパニー | 希薄な過酸化水素を使用する核酸汚染の除去方法 |
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US5858650A (en) * | 1992-04-03 | 1999-01-12 | Abbott Laboratories | Methods for inactivating nucleotide sequences and metal chelates for use therein |
US6482584B1 (en) * | 1998-11-13 | 2002-11-19 | Regeneration Technologies, Inc. | Cyclic implant perfusion cleaning and passivation process |
US20040262146A1 (en) * | 2000-10-02 | 2004-12-30 | Platt Robert C. | Sterilization system plasma generation control |
US7071152B2 (en) * | 2003-05-30 | 2006-07-04 | Steris Inc. | Cleaning and decontamination formula for surfaces contaminated with prion-infected material |
CN200957200Y (zh) * | 2006-06-28 | 2007-10-10 | 叶大林 | 气化过氧化氢灭菌装置 |
CN101909756B (zh) * | 2007-11-20 | 2013-10-16 | 3M创新有限公司 | 样品制备和收集系统及方法 |
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- 2010-10-19 FR FR1058516A patent/FR2966056B1/fr active Active
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- 2011-10-18 CN CN201180044130.XA patent/CN103096937B/zh active Active
- 2011-10-18 WO PCT/IB2011/054619 patent/WO2012052913A1/en active Application Filing
- 2011-10-18 US US13/275,700 patent/US8765054B2/en active Active
- 2011-10-18 JP JP2013534431A patent/JP5758006B2/ja active Active
- 2011-10-18 ES ES11776585.9T patent/ES2553975T3/es active Active
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2003006650A1 (fr) * | 2001-07-09 | 2003-01-23 | Asahi Kasei Kabushiki Kaisha | Procede de purification d'acide nucleique au moyen d'un non-tisse et procede de detection |
WO2005007884A1 (en) * | 2003-07-15 | 2005-01-27 | The Secretary Of State For The Home Department | Use of ethilene oxide to provide dna dree items |
JP2009511016A (ja) * | 2005-10-07 | 2009-03-19 | ベクトン・ディキンソン・アンド・カンパニー | 希薄な過酸化水素を使用する核酸汚染の除去方法 |
EP1792631A1 (en) * | 2005-11-30 | 2007-06-06 | Roche Diagnostics GmbH | Process for DNA decontamination |
Non-Patent Citations (2)
Title |
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JPN5014000710; ARCHER E. , et. al.: 'Validation of a dual cycle ethylene oxide treatment technique to remove DNA from consumables used in' FORENSIC SCIENCE INTERNATIONAL: GENETICS Volume 4, 20100701, Pages 239-243, ELSEVIER * |
JPN5014000715; SHAW K. ,et.al.: 'Comparison of the effects of sterilisation techniques on subsequent DNA profiling' International Journal of Legal MedicineInternational Journal of Legal Medicine Volume 122, 200801, Pages 29-33 * |
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Publication number | Publication date |
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WO2012052913A1 (en) | 2012-04-26 |
FR2966056B1 (fr) | 2016-03-18 |
EP2629806A1 (en) | 2013-08-28 |
CN103096937A (zh) | 2013-05-08 |
EP2629806B1 (en) | 2015-08-26 |
FR2966056A1 (fr) | 2012-04-20 |
ES2553975T3 (es) | 2015-12-15 |
US20120095178A1 (en) | 2012-04-19 |
US8765054B2 (en) | 2014-07-01 |
JP5758006B2 (ja) | 2015-08-05 |
CN103096937B (zh) | 2014-12-10 |
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