JP2013545116A - 高度なイメージング特性を有する顕微鏡イメージング装置 - Google Patents
高度なイメージング特性を有する顕微鏡イメージング装置 Download PDFInfo
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Abstract
Description
Claims (26)
- 光センサのアレイを含むイメージ捕捉手段と、
約1mW未満の励起光を、少なくとも0.5mm2である視野内の対象物体に向けるように、そして、前記励起光によって引き起こされた落射蛍光エミッションを、光センサの前記アレイに向けるように構成された、光学機器とを備え、前記光学機器と、光センサのアレイとは、前記視野のイメージについて、少なくとも2.5μmの解像度を提供するために、それぞれ、前記対象物体に十分に近い、
落射蛍光顕微鏡。 - 前記光学機器は、対物レンズと、発光ダイオードと、CMOSイメージセンサアレイとを含み、それぞれが、サイズが1立方インチ(約16.39cm3)未満である統合されたハウジング内に含まれる、請求項1に記載の顕微鏡。
- 前記光学機器は、対物レンズと、発光ダイオードと、CMOSイメージセンサアレイとを含み、それぞれが、統合されたハウジング内に含まれ、ここで、前記光学機器と、光センサのアレイとは、重量が2グラム未満である、請求項1に記載の顕微鏡。
- 前記イメージ捕捉手段における、かつ、前記対象物体の、光学倍率は、5以下である、請求項1に記載の顕微鏡。
- 前記光学機器は、前記励起光を提供する光源と、光センサの前記アレイと、対物レンズとの間の、セルフアライメントを提供するように構成される、請求項1に記載の顕微鏡。
- 前記光学機器は、リアルタイムイメージングの間、光センサの前記アレイと、対物レンズとの間の距離を調節することによって、イメージのフォーカシングを提供するように構成される、請求項1に記載の顕微鏡。
- 少なくとも1つの光学フィルタ要素と、前記励起光を生成する光源とを更に含み、ここで、前記少なくとも1つの光学フィルタ要素と、前記光源と、光センサの前記アレイとは、前記顕微鏡から取り外される、及び前記顕微鏡に再取り付けされるように構成される、請求項1に記載の顕微鏡。
- 前記光学機器と、光センサの前記アレイとは、前記視野のイメージについて、少なくとも2.5μmの解像度を、少なくとも36Hzのレートにおいて提供するために、それぞれ、前記対象物体に十分に近い、請求項1に記載の顕微鏡。
- 6mW未満を提供するエネルギー源から励起光を生成するように構成された、光源と、
センサアレイを含むイメージング回路手段と、
少なくとも0.5mm2である視野について、少なくとも2.5μmのイメージ解像度を提供するために、前記光源、前記イメージセンサアレイ、及び対象物体に十分に接近して動作するように構成された、対物レンズと
を備える、落射蛍光顕微鏡。 - サイズが1立方インチ(約16.39cm3)未満である落射蛍光顕微鏡であって、前記顕微鏡は、
光を、イメージング対象を含む視野に向けるように構成された、光励起配置と、
向けられた光と、前記イメージング対象との間の相互作用によって引き起こされた蛍光から、イメージデータを生成するように構成された、光センサアレイを含むイメージング回路と、
前記蛍光を、前記光センサアレイに、0.5mm2にわたって、かつ、少なくとも2.5μmの解像度で描かれるための前記イメージデータのため、十分な強度及びフォーカスを伴って向けるように構成された、光学配置と
を備える、顕微鏡。 - サイズが1立方インチ(約16.39cm3)未満であるポータブルハウジング
を備える、イメージング装置であって、前記ポータブルハウジングは、
励起光を提供する、励起源と、
第1の端と、第2の端とを有する、光学経路を提供する、細長い機器と
を備え、前記細長い機器は、
前記光学経路の前記第1の端における対物レンズと、
前記励起光を、前記対物レンズに向けるように構成及び配置された、1つ以上の励起要素と、
前記対物レンズから受け取られた落射蛍光エミッション光から、前記光学経路の前記第2の端における焦点面を提供するように構成及び配置された、1つ以上のエミッション要素と
前記焦点面において配置された、かつ、前記落射蛍光エミッション光から、対象物体のイメージを捕捉するように構成及び配置された、光センサのアレイを含む、イメージング回路と
を含み、前記イメージは、複数の個々の毛細血管を捕捉するための十分な視野と、前記個々の毛細血管を相互に区別するための十分な解像度とを有する、イメージング装置。 - 前記対物レンズは、ほぼ平行にされた落射蛍光エミッション光を、前記光学経路内に向け、前記十分な解像度は、毛細血管を通って流れている個々の赤血球を区別する、又は解像することが可能である、請求項11に記載のイメージング装置。
- 光センサの前記アレイは、CMOSセンサを含む、請求項11に記載のイメージング装置。
- 前記細長い機器は、前記対物レンズと、光センサの前記アレイとの間の、前記光学経路内に配置された、アクロマティックレンズを更に含み、前記アクロマティックレンズは、前記対物レンズからの、平行にされた落射蛍光を受け取り、前記平行にされた落射蛍光を、光センサの前記アレイに対応する焦点面上にフォーカスさせるように構成される、請求項11に記載のイメージング装置。
- 前記励起源は、発光ダイオードである、請求項11に記載のイメージング装置。
- 前記細長い機器は、前記光学経路内に配置された、かつ、前記励起源からの前記励起光を、前記対物レンズに反射し、前記対物レンズからの前記落射蛍光エミッション光を通過させるように構成された、ダイクロイックミラーを更に含む、請求項11に記載のイメージング装置。
- 前記イメージング装置は、前記対象物体の連続したイメージを、少なくとも50Hzのレートで捕捉するように構成及び配置され、前記イメージは、複数の個々の毛細血管を捕捉するための十分な視野と、前記個々の毛細血管を相互に区別する、かつ、毛細血管を通って流れている個々の赤血球を区別するための、十分な解像度とを有する、請求項11に記載のイメージング装置。
- 前記励起源は、前記励起源を別の励起源と交換することを可能にする、締結要素を使用して、前記細長い機器に物理的に接続される、請求項11に記載のイメージング装置。
- 前記細長い機器は、前記対物レンズと、光センサの前記アレイとの間の、前記光学経路内に配置された、エミッションフィルタを更に含み、前記エミッションフィルタは、前記落射蛍光エミッション光の波長を含む、帯域通過を有するように構成される、請求項11に記載のイメージング装置。
- 前記細長い機器は、前記イメージのフォーカシングを可能にするために、前記光学経路内の光学要素を調節するように構成される、請求項11に記載のイメージング装置。
- 前記イメージング装置は、移動している生体の、インビボのイメージを捕捉するように構成される、請求項11に記載のイメージング装置。
- 捕捉されたイメージを、デジタルデータとして送信する、送信器回路を更に含む、請求項11に記載のイメージング装置。
- 前記ハウジングに結合された、別の光学システムへの、インタフェースを更に含む、請求項11に記載のイメージング装置。
- 前記装置は、外部光学データ記録/設定システムとインタフェースするための、同期化回路を含み、ここで、前記同期化回路は、前記イメージング装置と、前記外部光学データ記録/設定システムとの間で通信されるデータのための、同期化情報を提供する、フレームアクティブ信号を通信するように構成及び配置される、請求項11に記載のイメージング装置。
- 慢性実験の間の、共通イメージング位置の反復イメージングのための、正確な顕微鏡アライメントを可能にするために、支持構造物のベースプレートに、前記落射蛍光顕微鏡を取り付けるステップ、及び再度取り付けるステップを含む、請求項1に記載の顕微鏡装置を使用する方法。
- 慢性実験の間の、共通イメージング位置の反復イメージングのための、正確な顕微鏡アライメントを可能にするために、支持構造物のベースプレートに、前記落射蛍光顕微鏡を取り付けるステップ、及び再度取り付けるステップを含む、請求項10に記載のイメージング装置を使用する方法。
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WO2012027586A2 (en) | 2012-03-01 |
US11259703B2 (en) | 2022-03-01 |
US20160004063A1 (en) | 2016-01-07 |
US20160029893A1 (en) | 2016-02-04 |
AU2015272003B2 (en) | 2017-10-19 |
WO2012027586A3 (en) | 2013-11-14 |
AU2011293269B2 (en) | 2015-10-01 |
US20160033752A1 (en) | 2016-02-04 |
US9498135B2 (en) | 2016-11-22 |
CA2943966C (en) | 2019-02-19 |
GB2483963A (en) | 2012-03-28 |
US10813552B2 (en) | 2020-10-27 |
CA2809581C (en) | 2016-11-15 |
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