JP2013512676A - 無間質条件下におけるヒト胚性幹細胞からの機能的巨核球および血小板の大規模生成 - Google Patents

無間質条件下におけるヒト胚性幹細胞からの機能的巨核球および血小板の大規模生成 Download PDF

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JP2013512676A
JP2013512676A JP2012542230A JP2012542230A JP2013512676A JP 2013512676 A JP2013512676 A JP 2013512676A JP 2012542230 A JP2012542230 A JP 2012542230A JP 2012542230 A JP2012542230 A JP 2012542230A JP 2013512676 A JP2013512676 A JP 2013512676A
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platelets
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cells
tpo
scf
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JP2013512676A5 (enExample
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フェン リー，
シー−ジアン ルー，
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ステム セル アンド リジェネレイティブ メディスン インターナショナル， インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0644—Platelets; Megakaryocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/08—Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2311—Interleukin-11 (IL-11)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/26—Flt-3 ligand (CD135L, flk-2 ligand)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1394—Bone marrow stromal cells; whole marrow
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2012542230A 2009-12-04 2010-12-03 無間質条件下におけるヒト胚性幹細胞からの機能的巨核球および血小板の大規模生成 Pending JP2013512676A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US26693909P 2009-12-04 2009-12-04
US61/266,939 2009-12-04
US38416510P 2010-09-17 2010-09-17
US61/384,165 2010-09-17
PCT/US2010/058990 WO2011069127A1 (en) 2009-12-04 2010-12-03 Large scale generation of functional megakaryocytes and platelets from human embryonic stem cells under stromal-free conditions

Related Child Applications (1)

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JP2016086761A Division JP2016135138A (ja) 2009-12-04 2016-04-25 無間質条件下におけるヒト胚性幹細胞からの機能的巨核球および血小板の大規模生成

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JP2013512676A true JP2013512676A (ja) 2013-04-18
JP2013512676A5 JP2013512676A5 (enExample) 2014-01-16

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JP2012542230A Pending JP2013512676A (ja) 2009-12-04 2010-12-03 無間質条件下におけるヒト胚性幹細胞からの機能的巨核球および血小板の大規模生成
JP2016086761A Pending JP2016135138A (ja) 2009-12-04 2016-04-25 無間質条件下におけるヒト胚性幹細胞からの機能的巨核球および血小板の大規模生成
JP2019039160A Pending JP2019122385A (ja) 2009-12-04 2019-03-05 無間質条件下におけるヒト胚性幹細胞からの機能的巨核球および血小板の大規模生成
JP2022011675A Pending JP2022064953A (ja) 2009-12-04 2022-01-28 無間質条件下におけるヒト胚性幹細胞からの機能的巨核球および血小板の大規模生成

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JP2016086761A Pending JP2016135138A (ja) 2009-12-04 2016-04-25 無間質条件下におけるヒト胚性幹細胞からの機能的巨核球および血小板の大規模生成
JP2019039160A Pending JP2019122385A (ja) 2009-12-04 2019-03-05 無間質条件下におけるヒト胚性幹細胞からの機能的巨核球および血小板の大規模生成
JP2022011675A Pending JP2022064953A (ja) 2009-12-04 2022-01-28 無間質条件下におけるヒト胚性幹細胞からの機能的巨核球および血小板の大規模生成

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US (2) US9988603B2 (enExample)
EP (1) EP2507359A4 (enExample)
JP (4) JP2013512676A (enExample)
KR (4) KR20190142424A (enExample)
AU (4) AU2010325811A1 (enExample)
BR (1) BR112012013497A2 (enExample)
CA (2) CA2782013C (enExample)
IN (1) IN2012DN05054A (enExample)
RU (1) RU2012127810A (enExample)
WO (1) WO2011069127A1 (enExample)

Cited By (7)

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WO2017065280A1 (ja) * 2015-10-14 2017-04-20 株式会社メガカリオン 精製血小板の製造方法
US9988603B2 (en) 2009-12-04 2018-06-05 Stem Cell & Regenerative Medicine International Large scale generation of functional megakaryocytes and platelets from human embryonic stem cells under stromal-free conditions
US10894065B2 (en) 2012-12-21 2021-01-19 Astellas Institute For Regenerative Medicine Methods for production of platelets from pluripotent stem cells and compositions thereof
US11566228B2 (en) 2006-04-14 2023-01-31 Astellas Institute For Regenerative Medicine Hemangio-colony forming cells
US12097223B2 (en) 2011-11-30 2024-09-24 Astellas Institute For Regenerative Medicine Mesenchymal stromal cells and uses related thereto
US12209255B2 (en) 2012-07-12 2025-01-28 Astellas Institute For Regenerative Medicine Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof
US12465621B2 (en) 2011-11-30 2025-11-11 Astellas Institute For Regenerative Medicine Mesenchymal stromal cells and uses related thereto

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JP6580329B2 (ja) 2014-03-31 2019-09-25 シスメックス株式会社 未分化細胞から分化細胞および／または分化細胞の産生物を取得する方法
WO2015179301A1 (en) 2014-05-19 2015-11-26 Eleftherios Papoutsakis Megakaryocytic particles and microparticles for cell therapy & fate modification of stem and progenitor cells
AU2016242823A1 (en) * 2015-03-30 2017-09-21 Jeffrey Thomas Loh Methods for producing modified red blood cell compositions, compositions and uses thereof
AU2016308818B2 (en) 2015-08-18 2022-04-21 Astellas Institute For Regenerative Medicine Clinical formulations
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CA3178268A1 (en) 2020-05-11 2021-11-18 Jonathan Thon Compositions and methods related to megakaryocyte-derived extracellular vesicles

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Cited By (13)

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Publication number Priority date Publication date Assignee Title
US11566228B2 (en) 2006-04-14 2023-01-31 Astellas Institute For Regenerative Medicine Hemangio-colony forming cells
US9988603B2 (en) 2009-12-04 2018-06-05 Stem Cell & Regenerative Medicine International Large scale generation of functional megakaryocytes and platelets from human embryonic stem cells under stromal-free conditions
US12097223B2 (en) 2011-11-30 2024-09-24 Astellas Institute For Regenerative Medicine Mesenchymal stromal cells and uses related thereto
US12465621B2 (en) 2011-11-30 2025-11-11 Astellas Institute For Regenerative Medicine Mesenchymal stromal cells and uses related thereto
US12209255B2 (en) 2012-07-12 2025-01-28 Astellas Institute For Regenerative Medicine Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof
US10894065B2 (en) 2012-12-21 2021-01-19 Astellas Institute For Regenerative Medicine Methods for production of platelets from pluripotent stem cells and compositions thereof
US11400118B2 (en) 2012-12-21 2022-08-02 Astellas Institute For Regenerative Medicine Methods for production of platelets from pluripotent stem cells and compositions thereof
US12076347B2 (en) 2012-12-21 2024-09-03 Astellas Institute For Regenerative Medicine Methods for production of platelets from pluripotent stem cells and compositions thereof
US12109239B2 (en) 2012-12-21 2024-10-08 Astellas Institute For Regenerative Medicine Methods for production of human hemogenic endothelial cells from pluripotent stem cells and compositions thereof
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JP7287634B2 (ja) 2015-10-14 2023-06-06 株式会社メガカリオン 精製血小板の製造方法
WO2017065280A1 (ja) * 2015-10-14 2017-04-20 株式会社メガカリオン 精製血小板の製造方法

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US9988603B2 (en) 2018-06-05
EP2507359A1 (en) 2012-10-10
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AU2017201995B2 (en) 2019-06-06
CA2782013A1 (en) 2011-06-09
US20120315338A1 (en) 2012-12-13
KR20180086301A (ko) 2018-07-30
KR20210066020A (ko) 2021-06-04
US20190002829A1 (en) 2019-01-03
RU2012127810A (ru) 2014-01-10
AU2022200188A1 (en) 2022-02-10
IN2012DN05054A (enExample) 2015-10-09
WO2011069127A1 (en) 2011-06-09
KR20190142424A (ko) 2019-12-26
KR20120128605A (ko) 2012-11-27
AU2019203670B2 (en) 2021-10-14
JP2022064953A (ja) 2022-04-26
EP2507359A4 (en) 2015-05-13
CA3115233A1 (en) 2011-06-09
AU2010325811A1 (en) 2012-06-21
AU2019203670A1 (en) 2019-06-13
BR112012013497A2 (pt) 2016-05-31
AU2017201995A1 (en) 2017-04-13
CA2782013C (en) 2021-06-08
JP2019122385A (ja) 2019-07-25

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