JP2013509180A - 改善された分泌効率を有する組換えタンパク質の産生方法 - Google Patents

改善された分泌効率を有する組換えタンパク質の産生方法 Download PDF

Info

Publication number
JP2013509180A
JP2013509180A JP2012536916A JP2012536916A JP2013509180A JP 2013509180 A JP2013509180 A JP 2013509180A JP 2012536916 A JP2012536916 A JP 2012536916A JP 2012536916 A JP2012536916 A JP 2012536916A JP 2013509180 A JP2013509180 A JP 2013509180A
Authority
JP
Japan
Prior art keywords
vps10
protein
cell
host cell
yeast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP2012536916A
Other languages
English (en)
Japanese (ja)
Inventor
ミール，マイケル
リン，ヘピン
チヨイ，ビヨン−クオン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Publication of JP2013509180A publication Critical patent/JP2013509180A/ja
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2012536916A 2009-10-30 2010-10-25 改善された分泌効率を有する組換えタンパク質の産生方法 Withdrawn JP2013509180A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US25637909P 2009-10-30 2009-10-30
US61/256,379 2009-10-30
US35066810P 2010-06-02 2010-06-02
US61/350,668 2010-06-02
PCT/US2010/053903 WO2011053541A1 (en) 2009-10-30 2010-10-25 Methods for the production of recombinant proteins with improved secretion efficiencies

Publications (1)

Publication Number Publication Date
JP2013509180A true JP2013509180A (ja) 2013-03-14

Family

ID=43922476

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012536916A Withdrawn JP2013509180A (ja) 2009-10-30 2010-10-25 改善された分泌効率を有する組換えタンパク質の産生方法

Country Status (12)

Country Link
US (1) US20130011875A1 (cg-RX-API-DMAC7.html)
EP (1) EP2494053A4 (cg-RX-API-DMAC7.html)
JP (1) JP2013509180A (cg-RX-API-DMAC7.html)
KR (1) KR20140015137A (cg-RX-API-DMAC7.html)
CN (1) CN102686731A (cg-RX-API-DMAC7.html)
AU (1) AU2010313608A1 (cg-RX-API-DMAC7.html)
BR (1) BR112012009886A2 (cg-RX-API-DMAC7.html)
CA (1) CA2777487A1 (cg-RX-API-DMAC7.html)
IN (1) IN2012DN03823A (cg-RX-API-DMAC7.html)
MX (1) MX2012004993A (cg-RX-API-DMAC7.html)
RU (1) RU2012122166A (cg-RX-API-DMAC7.html)
WO (1) WO2011053541A1 (cg-RX-API-DMAC7.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020503882A (ja) * 2017-01-13 2020-02-06 ボルト スレッズ インコーポレイテッド エラストマータンパク質

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2494050A4 (en) * 2009-10-30 2013-10-30 Merck Sharp & Dohme GRANULOCYTIC COLONY STIMULATING FACTOR MADE IN GLYCOENGINEERED PICHIA PASTORIS
JP5932766B2 (ja) * 2011-03-11 2016-06-08 株式会社カネカ Ｖｐｓ遺伝子が破壊されている酵母を用いる異種タンパク質の製造方法
EP3358000A3 (en) 2012-01-05 2018-12-19 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
CN102559740B (zh) * 2012-02-25 2013-03-27 山东大学 一种提高酿酒酵母分泌表达异源蛋白的方法及专用酿酒酵母菌株
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
JP6454650B2 (ja) * 2013-03-06 2019-01-23 プロタリクス リミテッド ＴＮＦαポリペプチド阻害剤を発現する植物細胞及び薬学的に許容される担体を含む医薬組成物
WO2015004241A2 (en) 2013-07-10 2015-01-15 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
WO2016012468A1 (en) 2014-07-21 2016-01-28 Novartis Ag Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
EP3221455B1 (en) * 2014-11-20 2020-08-12 Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells
EP3332005B1 (en) * 2015-08-05 2021-09-22 Lonza Ltd Promoter variants
EP4286524A3 (en) 2017-07-31 2024-03-06 Melt&Marble AB Fungal cell with improved protein production capacity
SE2051317A1 (en) * 2020-11-11 2022-05-12 Nielsen Dina Petranovic A genetically modified yeast cell
CN113604373A (zh) * 2021-02-08 2021-11-05 江南大学 一种提高人源溶菌酶产量及酶活的毕赤酵母缺陷型菌株
CN113736818A (zh) * 2021-10-08 2021-12-03 江南大学 一种毕赤酵母中提高人源溶菌酶分泌效率及酶活的方法
CN113862242B (zh) * 2021-10-15 2024-06-25 江南大学 一种减弱液泡分选提高里氏木霉纤维素酶产量的方法
EP4194560A1 (en) 2021-12-08 2023-06-14 European Molecular Biology Laboratory Improved production of secreted proteins in fungal cells
CN115851468A (zh) * 2022-07-18 2023-03-28 江南大学 一种生产人酪蛋白巨肽的重组毕赤酵母及其应用
CN116478905B (zh) * 2023-02-15 2025-07-22 北京中医药大学 一种高尔基体体外培养方法及其在构建药物毒性筛选系统中的应用
WO2025000334A1 (zh) * 2023-06-29 2025-01-02 中国科学院深圳先进技术研究院 融合蛋白及其在制备代谢产物中的应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4818700A (en) 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
JP4969011B2 (ja) 2000-05-17 2012-07-04 田辺三菱製薬株式会社 酸性糖鎖減少蛋白質の製造方法および該製造された糖蛋白質
EP1522590B1 (en) 2000-06-28 2009-08-26 Glycofi, Inc. Methods for producing modified glycoproteins
US7598055B2 (en) 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7795002B2 (en) 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
US8697394B2 (en) 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
GB0121088D0 (en) 2001-08-31 2001-10-24 Cambridge Consultants Electric kettle
NZ569490A (en) 2001-12-27 2008-11-28 Glycofi Inc Methods to engineer mammalian-type carbohydrate structures
IL165717A0 (en) * 2002-06-26 2006-01-15 Flanders Interuniversity Inst A strain of methylotrophic yeast for producing proteins
US7705203B2 (en) 2002-11-12 2010-04-27 Purdue Research Foundation Benzoate inductible promoters
US7259007B2 (en) 2003-12-24 2007-08-21 Glycofi, Inc. Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
US7479389B2 (en) 2004-03-02 2009-01-20 Glycofi, Inc. ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration
EP1730293A2 (en) 2004-03-17 2006-12-13 Glycofi, Inc. Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast
CA2565125A1 (en) 2004-04-29 2005-11-10 Glycofi, Inc. Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins
US20060252069A1 (en) 2005-04-21 2006-11-09 Zhang Kunyan Pcr for mrsa sccmec typing
US7851438B2 (en) * 2006-05-19 2010-12-14 GlycoFi, Incorporated Erythropoietin compositions
WO2007136865A2 (en) 2006-05-19 2007-11-29 Glycofi, Inc Recombinant vectors

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020503882A (ja) * 2017-01-13 2020-02-06 ボルト スレッズ インコーポレイテッド エラストマータンパク質
US11858971B2 (en) 2017-01-13 2024-01-02 Bolt Threads, Inc. Elastomeric proteins

Also Published As

Publication number Publication date
CN102686731A (zh) 2012-09-19
RU2012122166A (ru) 2013-12-10
MX2012004993A (es) 2012-06-12
US20130011875A1 (en) 2013-01-10
CA2777487A1 (en) 2011-05-05
BR112012009886A2 (pt) 2015-09-15
KR20140015137A (ko) 2014-02-06
WO2011053541A1 (en) 2011-05-05
IN2012DN03823A (cg-RX-API-DMAC7.html) 2015-08-28
EP2494053A4 (en) 2013-10-30
AU2010313608A1 (en) 2012-06-07
EP2494053A1 (en) 2012-09-05

Similar Documents

Publication Publication Date Title
JP2013509180A (ja) 改善された分泌効率を有する組換えタンパク質の産生方法
US11827891B2 (en) Protease deficient filamentous fungal cells and methods of use thereof
US8440456B2 (en) Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins
EP3019602B1 (en) Multiple proteases deficient filamentous fungal cells and methods of use thereof
US7968312B2 (en) Production of polypeptides by improved secretion
CN101945998B (zh) 用于蛋白生产的载体和酵母菌株
US20100297738A1 (en) Expression system
CN113423836A (zh) 重组宿主细胞中碳源调节的蛋白的产生
CN102725396A (zh) 在缺乏二肽基氨肽酶活性的巴斯德毕赤酵母中生产治疗性蛋白的方法
MXPA03004853A (es) Metodos y composiciones para produccion sumamente eficiente de proteinas heterologas en levadura.
JP4955764B2 (ja) 酵母発現系における組み換え外来タンパク質の分泌効率を増強するための方法
DK2646558T3 (en) Promoters for expression of genes in a fungal cell
CN113316641A (zh) 串联蛋白表达
KR100977446B1 (ko) 분비 스트레스 반응을 조절하는 한세눌라 폴리모르파의신규한 유전자 및 상기 유전자를 이용하여 재조합 단백질의분비 발현 효율을 증가시키는 방법
HK40054115A (en) Carbon-source regulated protein production in a recombinant host cell

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20130611

A761 Written withdrawal of application

Free format text: JAPANESE INTERMEDIATE CODE: A761

Effective date: 20140724