IN2012DN03823A - - Google Patents
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- Publication number
- IN2012DN03823A IN2012DN03823A IN3823DEN2012A IN2012DN03823A IN 2012DN03823 A IN2012DN03823 A IN 2012DN03823A IN 3823DEN2012 A IN3823DEN2012 A IN 3823DEN2012A IN 2012DN03823 A IN2012DN03823 A IN 2012DN03823A
- Authority
- IN
- India
- Prior art keywords
- modified
- vacuolar sorting
- vps10
- sorting activity
- host cell
- Prior art date
Links
- 230000000694 effects Effects 0.000 abstract 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 210000005253 yeast cell Anatomy 0.000 abstract 2
- 241000235058 Komagataella pastoris Species 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25637909P | 2009-10-30 | 2009-10-30 | |
| US35066810P | 2010-06-02 | 2010-06-02 | |
| PCT/US2010/053903 WO2011053541A1 (en) | 2009-10-30 | 2010-10-25 | Methods for the production of recombinant proteins with improved secretion efficiencies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IN2012DN03823A true IN2012DN03823A (cg-RX-API-DMAC7.html) | 2015-08-28 |
Family
ID=43922476
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IN3823DEN2012 IN2012DN03823A (cg-RX-API-DMAC7.html) | 2009-10-30 | 2010-10-25 |
Country Status (12)
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2494050A4 (en) * | 2009-10-30 | 2013-10-30 | Merck Sharp & Dohme | GRANULOCYTIC COLONY STIMULATING FACTOR MADE IN GLYCOENGINEERED PICHIA PASTORIS |
| JP5932766B2 (ja) * | 2011-03-11 | 2016-06-08 | 株式会社カネカ | Ｖｐｓ遺伝子が破壊されている酵母を用いる異種タンパク質の製造方法 |
| EP3358000A3 (en) | 2012-01-05 | 2018-12-19 | Glykos Finland Oy | Protease deficient filamentous fungal cells and methods of use thereof |
| CN102559740B (zh) * | 2012-02-25 | 2013-03-27 | 山东大学 | 一种提高酿酒酵母分泌表达异源蛋白的方法及专用酿酒酵母菌株 |
| DK2852610T3 (en) | 2012-05-23 | 2018-09-03 | Glykos Finland Oy | PRODUCTION OF FUCOSYLED GLYCOPROTEIN |
| JP6454650B2 (ja) * | 2013-03-06 | 2019-01-23 | プロタリクス リミテッド | ＴＮＦαポリペプチド阻害剤を発現する植物細胞及び薬学的に許容される担体を含む医薬組成物 |
| WO2015004241A2 (en) | 2013-07-10 | 2015-01-15 | Novartis Ag | Multiple proteases deficient filamentous fungal cells and methods of use thereof |
| WO2016012468A1 (en) | 2014-07-21 | 2016-01-28 | Novartis Ag | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
| EP3221455B1 (en) * | 2014-11-20 | 2020-08-12 | Yissum Research and Development Company of the Hebrew University of Jerusalem Ltd. | Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells |
| EP3332005B1 (en) * | 2015-08-05 | 2021-09-22 | Lonza Ltd | Promoter variants |
| WO2018132821A2 (en) | 2017-01-13 | 2018-07-19 | Bolt Threads, Inc. | Elastomeric proteins |
| EP4286524A3 (en) | 2017-07-31 | 2024-03-06 | Melt&Marble AB | Fungal cell with improved protein production capacity |
| SE2051317A1 (en) * | 2020-11-11 | 2022-05-12 | Nielsen Dina Petranovic | A genetically modified yeast cell |
| CN113604373A (zh) * | 2021-02-08 | 2021-11-05 | 江南大学 | 一种提高人源溶菌酶产量及酶活的毕赤酵母缺陷型菌株 |
| CN113736818A (zh) * | 2021-10-08 | 2021-12-03 | 江南大学 | 一种毕赤酵母中提高人源溶菌酶分泌效率及酶活的方法 |
| CN113862242B (zh) * | 2021-10-15 | 2024-06-25 | 江南大学 | 一种减弱液泡分选提高里氏木霉纤维素酶产量的方法 |
| EP4194560A1 (en) | 2021-12-08 | 2023-06-14 | European Molecular Biology Laboratory | Improved production of secreted proteins in fungal cells |
| CN115851468A (zh) * | 2022-07-18 | 2023-03-28 | 江南大学 | 一种生产人酪蛋白巨肽的重组毕赤酵母及其应用 |
| CN116478905B (zh) * | 2023-02-15 | 2025-07-22 | 北京中医药大学 | 一种高尔基体体外培养方法及其在构建药物毒性筛选系统中的应用 |
| WO2025000334A1 (zh) * | 2023-06-29 | 2025-01-02 | 中国科学院深圳先进技术研究院 | 融合蛋白及其在制备代谢产物中的应用 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| JP4969011B2 (ja) | 2000-05-17 | 2012-07-04 | 田辺三菱製薬株式会社 | 酸性糖鎖減少蛋白質の製造方法および該製造された糖蛋白質 |
| EP1522590B1 (en) | 2000-06-28 | 2009-08-26 | Glycofi, Inc. | Methods for producing modified glycoproteins |
| US7598055B2 (en) | 2000-06-28 | 2009-10-06 | Glycofi, Inc. | N-acetylglucosaminyltransferase III expression in lower eukaryotes |
| US7625756B2 (en) | 2000-06-28 | 2009-12-01 | GycoFi, Inc. | Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells |
| US7449308B2 (en) | 2000-06-28 | 2008-11-11 | Glycofi, Inc. | Combinatorial DNA library for producing modified N-glycans in lower eukaryotes |
| US7795002B2 (en) | 2000-06-28 | 2010-09-14 | Glycofi, Inc. | Production of galactosylated glycoproteins in lower eukaryotes |
| US8697394B2 (en) | 2000-06-28 | 2014-04-15 | Glycofi, Inc. | Production of modified glycoproteins having multiple antennary structures |
| US7863020B2 (en) | 2000-06-28 | 2011-01-04 | Glycofi, Inc. | Production of sialylated N-glycans in lower eukaryotes |
| GB0121088D0 (en) | 2001-08-31 | 2001-10-24 | Cambridge Consultants | Electric kettle |
| NZ569490A (en) | 2001-12-27 | 2008-11-28 | Glycofi Inc | Methods to engineer mammalian-type carbohydrate structures |
| IL165717A0 (en) * | 2002-06-26 | 2006-01-15 | Flanders Interuniversity Inst | A strain of methylotrophic yeast for producing proteins |
| US7705203B2 (en) | 2002-11-12 | 2010-04-27 | Purdue Research Foundation | Benzoate inductible promoters |
| US7259007B2 (en) | 2003-12-24 | 2007-08-21 | Glycofi, Inc. | Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins |
| US7479389B2 (en) | 2004-03-02 | 2009-01-20 | Glycofi, Inc. | ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration |
| EP1730293A2 (en) | 2004-03-17 | 2006-12-13 | Glycofi, Inc. | Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast |
| CA2565125A1 (en) | 2004-04-29 | 2005-11-10 | Glycofi, Inc. | Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins |
| US20060252069A1 (en) | 2005-04-21 | 2006-11-09 | Zhang Kunyan | Pcr for mrsa sccmec typing |
| US7851438B2 (en) * | 2006-05-19 | 2010-12-14 | GlycoFi, Incorporated | Erythropoietin compositions |
| WO2007136865A2 (en) | 2006-05-19 | 2007-11-29 | Glycofi, Inc | Recombinant vectors |
-
2010
- 2010-10-25 JP JP2012536916A patent/JP2013509180A/ja not_active Withdrawn
- 2010-10-25 RU RU2012122166/10A patent/RU2012122166A/ru not_active Application Discontinuation
- 2010-10-25 WO PCT/US2010/053903 patent/WO2011053541A1/en not_active Ceased
- 2010-10-25 CA CA2777487A patent/CA2777487A1/en not_active Abandoned
- 2010-10-25 KR KR1020127010917A patent/KR20140015137A/ko not_active Withdrawn
- 2010-10-25 IN IN3823DEN2012 patent/IN2012DN03823A/en unknown
- 2010-10-25 CN CN2010800519511A patent/CN102686731A/zh active Pending
- 2010-10-25 BR BR112012009886A patent/BR112012009886A2/pt not_active IP Right Cessation
- 2010-10-25 AU AU2010313608A patent/AU2010313608A1/en not_active Abandoned
- 2010-10-25 EP EP20100827360 patent/EP2494053A4/en not_active Withdrawn
- 2010-10-25 US US13/503,707 patent/US20130011875A1/en not_active Abandoned
- 2010-10-25 MX MX2012004993A patent/MX2012004993A/es not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| CN102686731A (zh) | 2012-09-19 |
| RU2012122166A (ru) | 2013-12-10 |
| MX2012004993A (es) | 2012-06-12 |
| US20130011875A1 (en) | 2013-01-10 |
| CA2777487A1 (en) | 2011-05-05 |
| BR112012009886A2 (pt) | 2015-09-15 |
| KR20140015137A (ko) | 2014-02-06 |
| JP2013509180A (ja) | 2013-03-14 |
| WO2011053541A1 (en) | 2011-05-05 |
| EP2494053A4 (en) | 2013-10-30 |
| AU2010313608A1 (en) | 2012-06-07 |
| EP2494053A1 (en) | 2012-09-05 |
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