JP2013507135A5 - - Google Patents
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- JP2013507135A5 JP2013507135A5 JP2012533443A JP2012533443A JP2013507135A5 JP 2013507135 A5 JP2013507135 A5 JP 2013507135A5 JP 2012533443 A JP2012533443 A JP 2012533443A JP 2012533443 A JP2012533443 A JP 2012533443A JP 2013507135 A5 JP2013507135 A5 JP 2013507135A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- progenitor cells
- culture medium
- culturing
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 claims description 61
- 210000000130 stem cell Anatomy 0.000 claims description 48
- 239000001963 growth medium Substances 0.000 claims description 40
- 210000004027 cell Anatomy 0.000 claims description 35
- 238000012258 culturing Methods 0.000 claims description 23
- 210000001778 pluripotent stem cell Anatomy 0.000 claims description 13
- 210000001900 endoderm Anatomy 0.000 claims description 12
- 210000001654 germ layer Anatomy 0.000 claims description 10
- 230000001939 inductive effect Effects 0.000 claims description 10
- 210000005155 neural progenitor cell Anatomy 0.000 claims description 8
- 238000004115 adherent culture Methods 0.000 claims description 6
- 238000010899 nucleation Methods 0.000 claims description 6
- 210000002242 embryoid body Anatomy 0.000 claims description 5
- 210000003716 mesoderm Anatomy 0.000 claims description 5
- 239000000872 buffer Substances 0.000 claims description 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 210000003981 ectoderm Anatomy 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 claims description 2
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 claims description 2
- 102000004877 Insulin Human genes 0.000 claims description 2
- 108090001061 Insulin Proteins 0.000 claims description 2
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 2
- 239000003242 anti bacterial agent Substances 0.000 claims description 2
- 229940088710 antibiotic agent Drugs 0.000 claims description 2
- 210000001130 astrocyte Anatomy 0.000 claims description 2
- 239000007853 buffer solution Substances 0.000 claims description 2
- 239000007975 buffered saline Substances 0.000 claims description 2
- 210000004413 cardiac myocyte Anatomy 0.000 claims description 2
- 210000001612 chondrocyte Anatomy 0.000 claims description 2
- 238000000151 deposition Methods 0.000 claims description 2
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 2
- 239000003797 essential amino acid Substances 0.000 claims description 2
- 235000020776 essential amino acid Nutrition 0.000 claims description 2
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims description 2
- 210000003494 hepatocyte Anatomy 0.000 claims description 2
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims description 2
- 229940125396 insulin Drugs 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 claims description 2
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims description 2
- 239000007758 minimum essential medium Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 210000002569 neuron Anatomy 0.000 claims description 2
- 210000004248 oligodendroglia Anatomy 0.000 claims description 2
- 239000011669 selenium Substances 0.000 claims description 2
- 229910052711 selenium Inorganic materials 0.000 claims description 2
- 235000011649 selenium Nutrition 0.000 claims description 2
- 210000002363 skeletal muscle cell Anatomy 0.000 claims description 2
- 239000011573 trace mineral Substances 0.000 claims description 2
- 235000013619 trace mineral Nutrition 0.000 claims description 2
- 239000011782 vitamin Substances 0.000 claims description 2
- 229940088594 vitamin Drugs 0.000 claims description 2
- 229930003231 vitamin Natural products 0.000 claims description 2
- 235000013343 vitamin Nutrition 0.000 claims description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims description 2
- 239000002243 precursor Substances 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25113009P | 2009-10-13 | 2009-10-13 | |
| US61/251,130 | 2009-10-13 | ||
| US35494710P | 2010-06-15 | 2010-06-15 | |
| US61/354,947 | 2010-06-15 | ||
| PCT/CA2010/001610 WO2011044684A1 (en) | 2009-10-13 | 2010-10-13 | Manipulation of osmolality for differentiating stem cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013507135A JP2013507135A (ja) | 2013-03-04 |
| JP2013507135A5 true JP2013507135A5 (https=) | 2013-11-21 |
| JP5843775B2 JP5843775B2 (ja) | 2016-01-13 |
Family
ID=43855139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012533443A Active JP5843775B2 (ja) | 2009-10-13 | 2010-10-13 | 幹細胞を分化させるための重量オスモル濃度の操作法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10913929B2 (https=) |
| EP (1) | EP2488631B1 (https=) |
| JP (1) | JP5843775B2 (https=) |
| CN (1) | CN102762718B (https=) |
| AU (1) | AU2010306377B2 (https=) |
| IL (1) | IL218996A (https=) |
| WO (1) | WO2011044684A1 (https=) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9708582B2 (en) * | 2009-10-13 | 2017-07-18 | Stemcell Technologies Inc. | Method of differentiating stem cells |
| MY191870A (en) | 2010-06-11 | 2022-07-18 | Regeneron Pharma | Production of fertile xy female animals from xy es cells |
| CN102851314A (zh) * | 2011-07-01 | 2013-01-02 | 中国科学院上海药物研究所 | 诱导性多能干细胞的制备方法和用于制备诱导性多能干细胞的培养基 |
| EP3693455A1 (en) * | 2011-11-04 | 2020-08-12 | Memorial Sloan-Kettering Cancer Center | Midbrain dopamine (da) neurons for engraftment |
| EP2841067A4 (en) * | 2012-04-25 | 2016-04-13 | Univ California | MEDICINAL SCREENING PLATFORM FOR THE RETT SYNDROME |
| WO2014143383A1 (en) | 2013-03-13 | 2014-09-18 | Agilent Technologies, Inc. | Transposome tethered to a gene delivery vehicle |
| ES2806698T3 (es) * | 2013-06-11 | 2021-02-18 | Ncardia B V | Composiciones de medio de cultivo para la maduración de cardiomiocitos derivados de células madre pluripotentes de mamíferos |
| KR102170502B1 (ko) | 2013-12-11 | 2020-10-28 | 리제너론 파마슈티칼스 인코포레이티드 | 게놈의 표적화된 변형을 위한 방법 및 조성물 |
| SI3161128T1 (sl) | 2014-06-26 | 2019-02-28 | Regeneron Pharmaceuticals, Inc. | Postopki in sestavki za ciljano genetsko spremembo in postopki uporabe |
| CN104293730B (zh) * | 2014-09-23 | 2017-07-28 | 云南中科灵长类生物医学重点实验室 | 多能干细胞体外定向分化为心肌细胞的方法 |
| BR112017007770A2 (pt) * | 2014-10-15 | 2018-01-16 | Regeneron Pharma | cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc. |
| WO2016086092A1 (en) | 2014-11-25 | 2016-06-02 | The Penn State Research Foundation | Chemical reprogramming of human glial cells into neurons for brain and spinal cord repair |
| CN104782583B (zh) * | 2015-04-29 | 2017-04-12 | 中国农业科学院兰州兽医研究所 | 一种棘球蚴体外三维培养模型及其应用 |
| MX388475B (es) | 2015-09-17 | 2025-03-20 | Regeneron Pharma | Selección de células pluripotentes para producir ratones hembra xy fértiles. |
| LT3374509T (lt) * | 2015-11-12 | 2021-03-10 | F. Hoffmann-La Roche Ag | Oligonukleotidai, skirti sukelti iš tėvo paveldėto ube3a raišką |
| CN105886457A (zh) * | 2016-05-16 | 2016-08-24 | 中国人民解放军军事医学科学院野战输血研究所 | 诱导干细胞分化成外胚层祖细胞的方法 |
| US11180733B2 (en) | 2016-07-04 | 2021-11-23 | Agency For Science, Technology And Research | Method of generating mesenchymal stem cells and uses thereof |
| KR101810012B1 (ko) | 2017-10-23 | 2018-01-18 | 차의과학대학교 산학협력단 | 히스톤 데아세틸라제 저해제 및 골 형성 단백질 경로 차단제를 이용한 인간-유래의 체세포로부터 역분화 만능 줄기세포의 제조방법 |
| KR102167257B1 (ko) * | 2019-05-28 | 2020-10-19 | 부산대학교 산학협력단 | 토마티딘 처리를 통한 줄기세포의 성숙화된 심근세포로의 분화유도 촉진 방법 |
| EP4019549B1 (en) | 2019-08-20 | 2025-06-04 | Orizuru Therapeutics, Inc. | Method for enriching cardiac myocytes |
| WO2021145322A1 (ja) * | 2020-01-14 | 2021-07-22 | 味の素株式会社 | 浸透圧低減培地 |
| CA3175071A1 (en) | 2020-03-11 | 2021-09-16 | Bit Bio Limited | Method of generating hepatic cells |
| CN114621920B (zh) * | 2022-02-22 | 2023-09-05 | 中国医学科学院血液病医院(中国医学科学院血液学研究所) | 一种由造血干祖细胞向nk细胞分化的方法及培养基 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5221537A (en) * | 1988-09-02 | 1993-06-22 | Gerald Hecht | Tissue irrigating solutions |
| US7439064B2 (en) | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
| US20020114788A1 (en) * | 2000-07-27 | 2002-08-22 | Ole Isacson | Cell implantation therapy for neurological diseases or disorders |
| US20060040385A1 (en) * | 2002-12-05 | 2006-02-23 | Technion Research & Development Foundation Ltd. | Cultured human pancreatic islets, and uses thereof |
| WO2006019366A1 (en) * | 2003-03-28 | 2006-02-23 | Wisconsin Alumni Research Foundation | Physiochemical culture conditions for embryonic stem cells |
| US8460928B2 (en) | 2005-10-24 | 2013-06-11 | Agency For Science, Technology And Research | Methods of specifying mesodermal, endodermal and mesoendodermal cell fates |
| GB0623635D0 (en) * | 2006-11-27 | 2007-01-03 | Stem Cell Sciences Uk Ltd | Pluripotent cell growth media |
| CA2901105C (en) * | 2007-01-03 | 2018-02-13 | California Stem Cell, Inc. | Stem cell growth media and methods of making and using same |
| EP2115127A4 (en) | 2007-01-17 | 2010-04-07 | Life Technologies Corp | METHOD AND COMPOSITIONS FOR IMPROVING CELL HEALTH IN CULTURES |
| FI20075417A0 (fi) | 2007-06-05 | 2007-06-05 | Marjo-Riitta Suuronen | Koostumuksia ja menetelmiä alkion kantasolujen kasvatukseen |
| AU2009290563A1 (en) | 2008-09-15 | 2010-03-18 | Genentech, Inc. | Compositions and methods for regulating cell osmolarity |
-
2010
- 2010-10-13 JP JP2012533443A patent/JP5843775B2/ja active Active
- 2010-10-13 CN CN201080056644.2A patent/CN102762718B/zh active Active
- 2010-10-13 US US12/903,521 patent/US10913929B2/en active Active
- 2010-10-13 EP EP10822954.3A patent/EP2488631B1/en active Active
- 2010-10-13 WO PCT/CA2010/001610 patent/WO2011044684A1/en not_active Ceased
- 2010-10-13 AU AU2010306377A patent/AU2010306377B2/en active Active
-
2012
- 2012-04-02 IL IL218996A patent/IL218996A/en active IP Right Grant
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