AU2010306377B2 - Manipulation of osmolality for differentiating stem cells - Google Patents

Manipulation of osmolality for differentiating stem cells Download PDF

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Publication number
AU2010306377B2
AU2010306377B2 AU2010306377A AU2010306377A AU2010306377B2 AU 2010306377 B2 AU2010306377 B2 AU 2010306377B2 AU 2010306377 A AU2010306377 A AU 2010306377A AU 2010306377 A AU2010306377 A AU 2010306377A AU 2010306377 B2 AU2010306377 B2 AU 2010306377B2
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cells
mosm
ebs
mtesr
culture
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AU2010306377A1 (en
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Alexandra A. Blak
Sharon A. Louis
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Stemcell Technologies Canada Inc
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Stemcell Technologies Canada Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/32—Polylysine, polyornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2010306377A 2009-10-13 2010-10-13 Manipulation of osmolality for differentiating stem cells Active AU2010306377B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US25113009P 2009-10-13 2009-10-13
US61/251,130 2009-10-13
US35494710P 2010-06-15 2010-06-15
US61/354,947 2010-06-15
PCT/CA2010/001610 WO2011044684A1 (en) 2009-10-13 2010-10-13 Manipulation of osmolality for differentiating stem cells

Publications (2)

Publication Number Publication Date
AU2010306377A1 AU2010306377A1 (en) 2012-05-10
AU2010306377B2 true AU2010306377B2 (en) 2015-01-22

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2010306377A Active AU2010306377B2 (en) 2009-10-13 2010-10-13 Manipulation of osmolality for differentiating stem cells

Country Status (7)

Country Link
US (1) US10913929B2 (https=)
EP (1) EP2488631B1 (https=)
JP (1) JP5843775B2 (https=)
CN (1) CN102762718B (https=)
AU (1) AU2010306377B2 (https=)
IL (1) IL218996A (https=)
WO (1) WO2011044684A1 (https=)

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US9708582B2 (en) * 2009-10-13 2017-07-18 Stemcell Technologies Inc. Method of differentiating stem cells
US9149026B2 (en) 2010-06-11 2015-10-06 Regeneron Pharmaceuticals, Inc. Production of fertile XY animals from XY ES cells
CN102851314A (zh) * 2011-07-01 2013-01-02 中国科学院上海药物研究所 诱导性多能干细胞的制备方法和用于制备诱导性多能干细胞的培养基
PT2773748T (pt) * 2011-11-04 2020-03-26 Memorial Sloan Kettering Cancer Center Neurónios dopaminérgicos (da) do mesencéfalo para enxerto
WO2013163455A2 (en) * 2012-04-25 2013-10-31 The Regents Of The University Of California A drug screening platform for rett syndrome
WO2014143383A1 (en) 2013-03-13 2014-09-18 Agilent Technologies, Inc. Transposome tethered to a gene delivery vehicle
WO2014200339A1 (en) * 2013-06-11 2014-12-18 Pluriomics B.V. Culture medium compositions for maturating cardiomyocytes derived from pluripotent mammalian stem cells
US9546384B2 (en) 2013-12-11 2017-01-17 Regeneron Pharmaceuticals, Inc. Methods and compositions for the targeted modification of a mouse genome
KR102386101B1 (ko) 2014-06-26 2022-04-14 리제너론 파마슈티칼스 인코포레이티드 표적화된 유전자 변형을 위한 방법 및 그 조성물, 및 사용 방법
CN104293730B (zh) * 2014-09-23 2017-07-28 云南中科灵长类生物医学重点实验室 多能干细胞体外定向分化为心肌细胞的方法
DK3207124T3 (da) * 2014-10-15 2019-08-12 Regeneron Pharma Fremgangsmåder og sammensætninger til generering eller bevaring af pluripotente celler
US9730975B2 (en) 2014-11-25 2017-08-15 The Penn State Research Foundation Chemical reprogramming of human glial cells into neurons for brain and spinal cord repair
CN104782583B (zh) * 2015-04-29 2017-04-12 中国农业科学院兰州兽医研究所 一种棘球蚴体外三维培养模型及其应用
SG10201913789VA (en) 2015-09-17 2020-03-30 Regeneron Pharma Selection of pluripotent cells for production of fertile xy female mice
HUE067905T2 (hu) * 2015-11-12 2024-11-28 Hoffmann La Roche Oligonukleotidok paternális UBE3A expresszió indukálására
CN105886457A (zh) * 2016-05-16 2016-08-24 中国人民解放军军事医学科学院野战输血研究所 诱导干细胞分化成外胚层祖细胞的方法
US11180733B2 (en) 2016-07-04 2021-11-23 Agency For Science, Technology And Research Method of generating mesenchymal stem cells and uses thereof
KR101810012B1 (ko) 2017-10-23 2018-01-18 차의과학대학교 산학협력단 히스톤 데아세틸라제 저해제 및 골 형성 단백질 경로 차단제를 이용한 인간-유래의 체세포로부터 역분화 만능 줄기세포의 제조방법
KR102167257B1 (ko) * 2019-05-28 2020-10-19 부산대학교 산학협력단 토마티딘 처리를 통한 줄기세포의 성숙화된 심근세포로의 분화유도 촉진 방법
CN114269900B (zh) * 2019-08-20 2025-01-14 千纸鹤治疗公司 心肌细胞的富集方法
KR20220129584A (ko) * 2020-01-14 2022-09-23 아지노모토 가부시키가이샤 삼투압 저감 배지
MX2022011245A (es) 2020-03-11 2023-01-11 Bit Bio Ltd Método de generación de células hepáticas.
CN114621920B (zh) * 2022-02-22 2023-09-05 中国医学科学院血液病医院(中国医学科学院血液学研究所) 一种由造血干祖细胞向nk细胞分化的方法及培养基

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WO2008085879A2 (en) * 2007-01-03 2008-07-17 California Stem Cell, Inc. Stem cell growth media and methods of making and using same
US20080286862A1 (en) * 2003-03-28 2008-11-20 Ludwig Tenneille E Physiochemical Culture Conditions for Embryonic Stem Cells
WO2008148938A1 (en) * 2007-06-05 2008-12-11 Kristiina Rajala Formulations and methods for culturing embryonic stem cells

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Also Published As

Publication number Publication date
CN102762718B (zh) 2015-06-17
AU2010306377A1 (en) 2012-05-10
CN102762718A (zh) 2012-10-31
JP2013507135A (ja) 2013-03-04
JP5843775B2 (ja) 2016-01-13
US20110086379A1 (en) 2011-04-14
US10913929B2 (en) 2021-02-09
EP2488631B1 (en) 2020-01-08
EP2488631A1 (en) 2012-08-22
WO2011044684A1 (en) 2011-04-21
EP2488631A4 (en) 2013-06-26
IL218996A (en) 2017-04-30
IL218996A0 (en) 2012-06-28

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Owner name: STEMCELL TECHNOLOGIES CANADA INC.

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Name of requester: HSBC BANK CANADA