CN102762718B - 用于分化干细胞的重量克分子渗透压浓度控制 - Google Patents

用于分化干细胞的重量克分子渗透压浓度控制 Download PDF

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CN102762718B
CN102762718B CN201080056644.2A CN201080056644A CN102762718B CN 102762718 B CN102762718 B CN 102762718B CN 201080056644 A CN201080056644 A CN 201080056644A CN 102762718 B CN102762718 B CN 102762718B
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cells
cell
ebs
mosm
osmolality
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CN102762718A (zh
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亚历山大·A·布莱克
莎朗·A·路易斯
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Stemcell Technologies Canada Inc
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/32—Polylysine, polyornithine
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Neurology (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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CN201080056644.2A 2009-10-13 2010-10-13 用于分化干细胞的重量克分子渗透压浓度控制 Active CN102762718B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US25113009P 2009-10-13 2009-10-13
US61/251,130 2009-10-13
US35494710P 2010-06-15 2010-06-15
US61/354,947 2010-06-15
PCT/CA2010/001610 WO2011044684A1 (en) 2009-10-13 2010-10-13 Manipulation of osmolality for differentiating stem cells

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CN102762718A CN102762718A (zh) 2012-10-31
CN102762718B true CN102762718B (zh) 2015-06-17

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US (1) US10913929B2 (https=)
EP (1) EP2488631B1 (https=)
JP (1) JP5843775B2 (https=)
CN (1) CN102762718B (https=)
AU (1) AU2010306377B2 (https=)
IL (1) IL218996A (https=)
WO (1) WO2011044684A1 (https=)

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US9708582B2 (en) * 2009-10-13 2017-07-18 Stemcell Technologies Inc. Method of differentiating stem cells
US9149026B2 (en) 2010-06-11 2015-10-06 Regeneron Pharmaceuticals, Inc. Production of fertile XY animals from XY ES cells
CN102851314A (zh) * 2011-07-01 2013-01-02 中国科学院上海药物研究所 诱导性多能干细胞的制备方法和用于制备诱导性多能干细胞的培养基
PT2773748T (pt) * 2011-11-04 2020-03-26 Memorial Sloan Kettering Cancer Center Neurónios dopaminérgicos (da) do mesencéfalo para enxerto
WO2013163455A2 (en) * 2012-04-25 2013-10-31 The Regents Of The University Of California A drug screening platform for rett syndrome
WO2014143383A1 (en) 2013-03-13 2014-09-18 Agilent Technologies, Inc. Transposome tethered to a gene delivery vehicle
WO2014200339A1 (en) * 2013-06-11 2014-12-18 Pluriomics B.V. Culture medium compositions for maturating cardiomyocytes derived from pluripotent mammalian stem cells
US9546384B2 (en) 2013-12-11 2017-01-17 Regeneron Pharmaceuticals, Inc. Methods and compositions for the targeted modification of a mouse genome
KR102386101B1 (ko) 2014-06-26 2022-04-14 리제너론 파마슈티칼스 인코포레이티드 표적화된 유전자 변형을 위한 방법 및 그 조성물, 및 사용 방법
CN104293730B (zh) * 2014-09-23 2017-07-28 云南中科灵长类生物医学重点实验室 多能干细胞体外定向分化为心肌细胞的方法
DK3207124T3 (da) * 2014-10-15 2019-08-12 Regeneron Pharma Fremgangsmåder og sammensætninger til generering eller bevaring af pluripotente celler
US9730975B2 (en) 2014-11-25 2017-08-15 The Penn State Research Foundation Chemical reprogramming of human glial cells into neurons for brain and spinal cord repair
CN104782583B (zh) * 2015-04-29 2017-04-12 中国农业科学院兰州兽医研究所 一种棘球蚴体外三维培养模型及其应用
SG10201913789VA (en) 2015-09-17 2020-03-30 Regeneron Pharma Selection of pluripotent cells for production of fertile xy female mice
HUE067905T2 (hu) * 2015-11-12 2024-11-28 Hoffmann La Roche Oligonukleotidok paternális UBE3A expresszió indukálására
CN105886457A (zh) * 2016-05-16 2016-08-24 中国人民解放军军事医学科学院野战输血研究所 诱导干细胞分化成外胚层祖细胞的方法
US11180733B2 (en) 2016-07-04 2021-11-23 Agency For Science, Technology And Research Method of generating mesenchymal stem cells and uses thereof
KR101810012B1 (ko) 2017-10-23 2018-01-18 차의과학대학교 산학협력단 히스톤 데아세틸라제 저해제 및 골 형성 단백질 경로 차단제를 이용한 인간-유래의 체세포로부터 역분화 만능 줄기세포의 제조방법
KR102167257B1 (ko) * 2019-05-28 2020-10-19 부산대학교 산학협력단 토마티딘 처리를 통한 줄기세포의 성숙화된 심근세포로의 분화유도 촉진 방법
CN114269900B (zh) * 2019-08-20 2025-01-14 千纸鹤治疗公司 心肌细胞的富集方法
KR20220129584A (ko) * 2020-01-14 2022-09-23 아지노모토 가부시키가이샤 삼투압 저감 배지
MX2022011245A (es) 2020-03-11 2023-01-11 Bit Bio Ltd Método de generación de células hepáticas.
CN114621920B (zh) * 2022-02-22 2023-09-05 中国医学科学院血液病医院(中国医学科学院血液学研究所) 一种由造血干祖细胞向nk细胞分化的方法及培养基

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AU2010306377A1 (en) 2012-05-10
CN102762718A (zh) 2012-10-31
JP2013507135A (ja) 2013-03-04
JP5843775B2 (ja) 2016-01-13
US20110086379A1 (en) 2011-04-14
US10913929B2 (en) 2021-02-09
EP2488631B1 (en) 2020-01-08
EP2488631A1 (en) 2012-08-22
AU2010306377B2 (en) 2015-01-22
WO2011044684A1 (en) 2011-04-21
EP2488631A4 (en) 2013-06-26
IL218996A (en) 2017-04-30
IL218996A0 (en) 2012-06-28

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