JP2013506416A - 細胞遊離のための方法及びキット - Google Patents

細胞遊離のための方法及びキット Download PDF

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Publication number
JP2013506416A
JP2013506416A JP2012532045A JP2012532045A JP2013506416A JP 2013506416 A JP2013506416 A JP 2013506416A JP 2012532045 A JP2012532045 A JP 2012532045A JP 2012532045 A JP2012532045 A JP 2012532045A JP 2013506416 A JP2013506416 A JP 2013506416A
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JP
Japan
Prior art keywords
cells
cell culture
layers
culture support
multilayer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
JP2012532045A
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English (en)
Japanese (ja)
Other versions
JP2013506416A5 (enExample
Inventor
ルビンシュタイン，スラウォミール
スミス，レジナルド・ディ
ロギン，イヴェリナ・アール
スサーラ，プラミーラ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Electric Co
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Electric Co filed Critical General Electric Co
Publication of JP2013506416A publication Critical patent/JP2013506416A/ja
Publication of JP2013506416A5 publication Critical patent/JP2013506416A5/ja
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/32—Polylysine, polyornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
JP2012532045A 2009-09-30 2010-09-27 細胞遊離のための方法及びキット Ceased JP2013506416A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/570,032 2009-09-30
US12/570,032 US8399252B2 (en) 2009-09-30 2009-09-30 Methods and kits for cell release
PCT/SE2010/051033 WO2011040867A1 (en) 2009-09-30 2010-09-27 Methods and kits for cell release

Publications (2)

Publication Number Publication Date
JP2013506416A true JP2013506416A (ja) 2013-02-28
JP2013506416A5 JP2013506416A5 (enExample) 2013-11-14

Family

ID=43780827

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012532045A Ceased JP2013506416A (ja) 2009-09-30 2010-09-27 細胞遊離のための方法及びキット

Country Status (7)

Country Link
US (2) US8399252B2 (enExample)
EP (1) EP2483391A4 (enExample)
JP (1) JP2013506416A (enExample)
CN (1) CN102575226A (enExample)
CA (1) CA2772248A1 (enExample)
IN (1) IN2012DN01891A (enExample)
WO (1) WO2011040867A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9453197B2 (en) 2010-12-16 2016-09-27 General Electric Company Methods of making cell carrier
US9926523B2 (en) 2010-12-16 2018-03-27 General Electric Company Cell carriers and methods for culturing cells
US9453196B2 (en) 2010-12-16 2016-09-27 General Electric Company Cell carrier, methods of making and use
US9534206B2 (en) 2010-12-16 2017-01-03 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
US9518249B2 (en) 2010-12-16 2016-12-13 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
US11148349B2 (en) 2013-09-27 2021-10-19 3M Innovative Properties Company Dual-sided structured film articles
JP7572567B2 (ja) * 2020-12-31 2024-10-23 アカデミア シニカ 細胞培養システム、方法およびその使用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7022522B2 (en) 1998-11-13 2006-04-04 Limin Guan Macroporous polymer scaffold containing calcium phosphate particles
JP4161106B2 (ja) 2004-09-30 2008-10-08 独立行政法人科学技術振興機構 細胞剥離剤及び細胞シート剥離方法
ITRM20060020A1 (it) 2006-01-18 2007-07-19 Andrea Masotti Metodo per la coltura di cellule
GB2425074A (en) * 2006-03-23 2006-10-18 Ilika Technologies Ltd Selective cellular adhesion on polymer microarrays
US20080241926A1 (en) 2007-03-02 2008-10-02 Ilsoon Lee Cell adhesion on surfaces of varying topographies

Also Published As

Publication number Publication date
US20110076764A1 (en) 2011-03-31
CA2772248A1 (en) 2011-04-07
EP2483391A4 (en) 2013-04-10
WO2011040867A1 (en) 2011-04-07
IN2012DN01891A (enExample) 2015-07-24
EP2483391A1 (en) 2012-08-08
US8993322B2 (en) 2015-03-31
US8399252B2 (en) 2013-03-19
CN102575226A (zh) 2012-07-11
US20130171729A1 (en) 2013-07-04

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