CN102575226A - 细胞释放的方法和试剂盒 - Google Patents

细胞释放的方法和试剂盒 Download PDF

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Publication number
CN102575226A
CN102575226A CN2010800450145A CN201080045014A CN102575226A CN 102575226 A CN102575226 A CN 102575226A CN 2010800450145 A CN2010800450145 A CN 2010800450145A CN 201080045014 A CN201080045014 A CN 201080045014A CN 102575226 A CN102575226 A CN 102575226A
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China
Prior art keywords
cells
cell culture
layer
multilayer
cell
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Pending
Application number
CN2010800450145A
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English (en)
Chinese (zh)
Inventor
S·鲁宾茨塔恩
R·D·史密斯
E·R·洛格欣
P·苏萨拉
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General Electric Co
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General Electric Co
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Publication date
Application filed by General Electric Co filed Critical General Electric Co
Publication of CN102575226A publication Critical patent/CN102575226A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/32—Polylysine, polyornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
CN2010800450145A 2009-09-30 2010-09-27 细胞释放的方法和试剂盒 Pending CN102575226A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/570,032 2009-09-30
US12/570,032 US8399252B2 (en) 2009-09-30 2009-09-30 Methods and kits for cell release
PCT/SE2010/051033 WO2011040867A1 (en) 2009-09-30 2010-09-27 Methods and kits for cell release

Publications (1)

Publication Number Publication Date
CN102575226A true CN102575226A (zh) 2012-07-11

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ID=43780827

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2010800450145A Pending CN102575226A (zh) 2009-09-30 2010-09-27 细胞释放的方法和试剂盒

Country Status (7)

Country Link
US (2) US8399252B2 (enExample)
EP (1) EP2483391A4 (enExample)
JP (1) JP2013506416A (enExample)
CN (1) CN102575226A (enExample)
CA (1) CA2772248A1 (enExample)
IN (1) IN2012DN01891A (enExample)
WO (1) WO2011040867A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115413296A (zh) * 2020-12-31 2022-11-29 刘扶东 细胞培养基质、其方法及用途

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9453197B2 (en) 2010-12-16 2016-09-27 General Electric Company Methods of making cell carrier
US9926523B2 (en) 2010-12-16 2018-03-27 General Electric Company Cell carriers and methods for culturing cells
US9453196B2 (en) 2010-12-16 2016-09-27 General Electric Company Cell carrier, methods of making and use
US9534206B2 (en) 2010-12-16 2017-01-03 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
US9518249B2 (en) 2010-12-16 2016-12-13 General Electric Company Cell carrier, associated methods for making cell carrier and culturing cells using the same
US11148349B2 (en) 2013-09-27 2021-10-19 3M Innovative Properties Company Dual-sided structured film articles

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080241926A1 (en) * 2007-03-02 2008-10-02 Ilsoon Lee Cell adhesion on surfaces of varying topographies

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7022522B2 (en) 1998-11-13 2006-04-04 Limin Guan Macroporous polymer scaffold containing calcium phosphate particles
JP4161106B2 (ja) 2004-09-30 2008-10-08 独立行政法人科学技術振興機構 細胞剥離剤及び細胞シート剥離方法
ITRM20060020A1 (it) 2006-01-18 2007-07-19 Andrea Masotti Metodo per la coltura di cellule
GB2425074A (en) * 2006-03-23 2006-10-18 Ilika Technologies Ltd Selective cellular adhesion on polymer microarrays

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080241926A1 (en) * 2007-03-02 2008-10-02 Ilsoon Lee Cell adhesion on surfaces of varying topographies

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CONSTANT VODOUHE等: "Control of drug accessibility on functional polyelectrolyte multilayer films", 《BIOMATERIALS》 *
J.F. LIANG等: "Dimethyl sulfoxide induces multilayer aggregates and prolongs survival of primary cultured hepatocytes", 《BIOTECHNOLOGY TECHNIQUES》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115413296A (zh) * 2020-12-31 2022-11-29 刘扶东 细胞培养基质、其方法及用途

Also Published As

Publication number Publication date
US20110076764A1 (en) 2011-03-31
CA2772248A1 (en) 2011-04-07
JP2013506416A (ja) 2013-02-28
EP2483391A4 (en) 2013-04-10
WO2011040867A1 (en) 2011-04-07
IN2012DN01891A (enExample) 2015-07-24
EP2483391A1 (en) 2012-08-08
US8993322B2 (en) 2015-03-31
US8399252B2 (en) 2013-03-19
US20130171729A1 (en) 2013-07-04

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Effective date of abandoning: 20120711

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