JP2013505728A5 - - Google Patents
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- JP2013505728A5 JP2013505728A5 JP2012531105A JP2012531105A JP2013505728A5 JP 2013505728 A5 JP2013505728 A5 JP 2013505728A5 JP 2012531105 A JP2012531105 A JP 2012531105A JP 2012531105 A JP2012531105 A JP 2012531105A JP 2013505728 A5 JP2013505728 A5 JP 2013505728A5
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- JP
- Japan
- Prior art keywords
- composition
- nanoparticles
- cell
- positively charged
- negatively charged
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000002105 nanoparticle Substances 0.000 claims 13
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 2
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 claims 2
- 229910000460 iron oxide Inorganic materials 0.000 claims 2
- 108010039918 Polylysine Proteins 0.000 claims 1
- 150000004676 glycans Polymers 0.000 claims 1
- 239000010931 gold Substances 0.000 claims 1
- 229910052737 gold Inorganic materials 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 229920000656 polylysine Polymers 0.000 claims 1
- 229920000642 polymer Polymers 0.000 claims 1
- 229920001282 polysaccharide Polymers 0.000 claims 1
- 239000005017 polysaccharide Substances 0.000 claims 1
- 150000004804 polysaccharides Polymers 0.000 claims 1
Claims (10)
- 細胞を磁性化するための組成物であって、
a)負に帯電したナノ粒子;
b)正に帯電したナノ粒子;及び
c)支持分子、
を含み、ここで前記負に帯電したナノ粒子又は正に帯電したナノ粒子のうちの1つが磁気応答性エレメントを含み、かつ、緊密な混合状態で前記支持分子が前記負に帯電したナノ粒子及び前記正に帯電したナノ粒子を保持する組成物。 - 前記負に帯電したナノ粒子が金のナノ粒子であり、前記正に帯電したナノ粒子が酸化鉄のナノ粒子である、請求項1に記載の組成物。
- 前記支持分子がペプチド、ポリサッカリド、核酸、ポリマー又はその組み合わせを含む、請求項1に記載の組成物。
- 前記支持分子がポリ−リシンを含む、請求項1に記載の組成物。
- a)前記支持分子がポリ−リシンを含み、
b)前記負に帯電したナノ粒子が金のナノ粒子であり、及び
c)前記正に帯電したナノ粒子が酸化鉄のナノ粒子である、請求項1に記載の組成物。 - 請求項1又は5に記載の組成物と細胞を接触させること、及び該細胞が磁性化するまで1〜12時間インキュベートすること、及び磁性化した前記細胞を、前記細胞を移動させるのに十分な磁場にかけることを含む、細胞を移動させる方法。
- 前記磁場が非対称である、請求項6に記載の方法。
- 前記細胞が懸濁されている又は接着している、請求項6に記載の方法。
- 請求項1又は5に記載の組成物とインキュベートすることにより作成される磁性化細胞を含む組成物。
- 前記細胞が凍結している、請求項9に記載の組成物。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US24584609P | 2009-09-25 | 2009-09-25 | |
US61/245,846 | 2009-09-25 | ||
PCT/US2010/050445 WO2011038370A1 (en) | 2009-09-25 | 2010-09-27 | Materials for magnetizing cells and magnetic manipulation |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2013505728A JP2013505728A (ja) | 2013-02-21 |
JP2013505728A5 true JP2013505728A5 (ja) | 2013-07-25 |
JP5769717B2 JP5769717B2 (ja) | 2015-08-26 |
Family
ID=43796264
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2012531105A Active JP5769717B2 (ja) | 2009-09-25 | 2010-09-27 | 細胞を磁性化するための材料及び磁気操作 |
Country Status (6)
Country | Link |
---|---|
US (2) | US8883471B2 (ja) |
EP (1) | EP2480343B1 (ja) |
JP (1) | JP5769717B2 (ja) |
BR (1) | BR112012006558B1 (ja) |
ES (1) | ES2657242T3 (ja) |
WO (1) | WO2011038370A1 (ja) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20160137974A1 (en) | 2008-09-25 | 2016-05-19 | Nano3D Biosciences, Inc | Microplates for magnetic 3d culture |
WO2011038370A1 (en) * | 2009-09-25 | 2011-03-31 | N3D Biosciences, Inc. | Materials for magnetizing cells and magnetic manipulation |
KR101878749B1 (ko) * | 2010-03-05 | 2018-07-17 | 삼성전자주식회사 | 표적 세포의 분리 방법 및 키트 |
ES2644243T3 (es) | 2011-02-01 | 2017-11-28 | Nano3D Biosciences, Inc. | Ensayo de viabilidad celular en 3D |
JP6466323B2 (ja) * | 2012-06-14 | 2019-02-06 | グライナー バイオ‐ワン ノース アメリカ,インコーポレイテッド | 磁性細胞外マトリックス |
TWI655017B (zh) * | 2012-11-29 | 2019-04-01 | 微美德桑提亞公司 | 含穩定磁場之方法、設備及分析方法 |
CN104232614B (zh) * | 2013-06-15 | 2017-12-15 | 长春理工大学 | 生理环境下细胞磁力显微操纵方法和系统 |
WO2015130745A1 (en) * | 2014-02-26 | 2015-09-03 | Brigham And Women's Hospital, Inc. | System and method for magnetic self-assembly |
KR102527270B1 (ko) | 2014-02-26 | 2023-04-27 | 브리검앤드위민즈하스피탈, 인코포레이티드 | 세포 레비테이션 및 모니터링을 위한 시스템 및 방법 |
US10092891B2 (en) | 2014-04-25 | 2018-10-09 | University Of Florida Research Foundation, Incorporated | Controlling the activity of growth factors, particularly TGF-β, in vivo |
JP6173421B2 (ja) * | 2015-12-17 | 2017-08-02 | ナノ3ディー バイオサイエンスィズ,インコーポレイテッド | 磁気三次元培養用機器 |
US10073346B2 (en) | 2016-07-08 | 2018-09-11 | Cypre, Inc. | Apparatus for patterning hydrogels into multi-well plates |
JP7212028B2 (ja) | 2017-07-13 | 2023-01-24 | グライナー バイオ‐ワン ノース アメリカ,インコーポレイテッド | 撮像用の培養プレート |
KR102001724B1 (ko) * | 2017-07-20 | 2019-07-18 | 서울대학교산학협력단 | 나노입자 어셈블리 구조체 및 이를 이용한 면역 분석 방법 |
US20200239833A1 (en) * | 2017-08-08 | 2020-07-30 | Greiner Bio-One North America, Inc. | Use of magnetic cells to manipulate non-magnetic cells |
KR102220208B1 (ko) * | 2018-06-22 | 2021-02-25 | 서울대학교산학협력단 | 나노입자 구조체 |
KR102176034B1 (ko) * | 2018-06-22 | 2020-11-06 | 서울대학교산학협력단 | 혈액 클렌징 시스템 |
JP7151218B2 (ja) * | 2018-07-04 | 2022-10-12 | 横河電機株式会社 | 細胞構築物の製造方法、担体、および担体の製造方法 |
CN112430564B (zh) * | 2019-08-26 | 2023-09-08 | 上海交通大学医学院附属第九人民医院 | 一种磁控三维细胞培养物调控方法 |
CN111944739B (zh) * | 2020-08-20 | 2021-11-26 | 创芯国际生物科技(广州)有限公司 | 一种类器官培养基质材料及其制备方法和应用 |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995031481A1 (en) | 1994-05-18 | 1995-11-23 | The Research And Development Institute, Inc. | Simple, rapid method for the detection, identification and enumeration of specific viable microorganisms |
US20020086842A1 (en) * | 2000-06-26 | 2002-07-04 | Christian Plank | Method for transfecting cells using a magnetic field |
EP1532274A4 (en) | 2002-06-28 | 2006-09-27 | Purdue Research Foundation | MAGNETIC NANOMATERIALS AND METHODS FOR DETECTING BIOLOGICAL MATERIALS |
WO2004083416A1 (ja) * | 2003-03-18 | 2004-09-30 | Japan Tissue Engineering Co., Ltd | 細胞シート移送方法及び細胞シート移送機器 |
GB0313259D0 (en) * | 2003-06-09 | 2003-07-16 | Consejo Superior Investigacion | Magnetic nanoparticles |
US20090137018A1 (en) * | 2003-06-30 | 2009-05-28 | University Of South Florida | Magnetic three-dimensional cell culture apparatus and method |
US7906345B2 (en) * | 2003-11-12 | 2011-03-15 | The Board Of Trustees Of The Leland Stanford Junior University | Magnetic nanoparticles, magnetic detector arrays, and methods for their use in detecting biological molecules |
JP5398982B2 (ja) * | 2004-05-24 | 2014-01-29 | ミダテック リミテッド | Rnaリガンドを含むナノ粒子 |
US7332101B2 (en) * | 2004-06-25 | 2008-02-19 | Massachusetts Institute Of Technology | Permanently linked, rigid, magnetic chains |
JP4863655B2 (ja) * | 2005-06-17 | 2012-01-25 | 国立大学法人 東京医科歯科大学 | 細胞含有シート |
AU2006288048A1 (en) * | 2005-09-08 | 2007-03-15 | Biterials Co., Ltd. | Magnetic nanoparticle having fluorescent and preparation method thereof and use thereof |
WO2011038370A1 (en) * | 2009-09-25 | 2011-03-31 | N3D Biosciences, Inc. | Materials for magnetizing cells and magnetic manipulation |
-
2010
- 2010-09-27 WO PCT/US2010/050445 patent/WO2011038370A1/en active Application Filing
- 2010-09-27 US US13/393,651 patent/US8883471B2/en active Active
- 2010-09-27 ES ES10819626.2T patent/ES2657242T3/es active Active
- 2010-09-27 JP JP2012531105A patent/JP5769717B2/ja active Active
- 2010-09-27 BR BR112012006558-0A patent/BR112012006558B1/pt active IP Right Grant
- 2010-09-27 EP EP10819626.2A patent/EP2480343B1/en active Active
-
2014
- 2014-11-07 US US14/536,388 patent/US9688955B2/en active Active
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