JP2013164419A5 - - Google Patents
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- Publication number
- JP2013164419A5 JP2013164419A5 JP2013024533A JP2013024533A JP2013164419A5 JP 2013164419 A5 JP2013164419 A5 JP 2013164419A5 JP 2013024533 A JP2013024533 A JP 2013024533A JP 2013024533 A JP2013024533 A JP 2013024533A JP 2013164419 A5 JP2013164419 A5 JP 2013164419A5
- Authority
- JP
- Japan
- Prior art keywords
- sample
- aqueous solution
- vitrified
- generating
- image
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 claims 17
- 238000000034 method Methods 0.000 claims 14
- 239000007864 aqueous solution Substances 0.000 claims 6
- 239000002245 particle Substances 0.000 claims 6
- 230000003287 optical effect Effects 0.000 claims 3
- 238000001816 cooling Methods 0.000 claims 2
- 239000012530 fluid Substances 0.000 claims 2
- 238000010884 ion-beam technique Methods 0.000 claims 2
- 238000007787 electrohydrodynamic spraying Methods 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 238000003801 milling Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 239000007921 spray Substances 0.000 claims 1
- 238000005507 spraying Methods 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12155112.1A EP2626885A1 (en) | 2012-02-13 | 2012-02-13 | Forming a vitrified sample for an electron microscopy |
| EP12155112.1 | 2012-02-13 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013164419A JP2013164419A (ja) | 2013-08-22 |
| JP2013164419A5 true JP2013164419A5 (enExample) | 2016-02-12 |
| JP6143487B2 JP6143487B2 (ja) | 2017-06-07 |
Family
ID=47631379
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013024533A Active JP6143487B2 (ja) | 2012-02-13 | 2013-02-12 | 電子顕微鏡用のガラス化された試料を作製する方法 |
Country Status (3)
| Country | Link |
|---|---|
| EP (2) | EP2626885A1 (enExample) |
| JP (1) | JP6143487B2 (enExample) |
| CN (1) | CN103247506B (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2881970A1 (en) * | 2013-12-04 | 2015-06-10 | Fei Company | Method of producing a freestanding thin film of nano-crystalline carbon |
| KR101729814B1 (ko) * | 2014-07-16 | 2017-04-24 | 주식회사 엘지화학 | Abs 수지 내의 그라프트된 스티렌-아크릴로니트릴 두께 측정 방법 |
| EP3125271B1 (en) * | 2015-07-29 | 2018-06-06 | FEI Company | Micro-chamber for inspecting sample material |
| KR101690670B1 (ko) * | 2015-10-14 | 2016-12-28 | 강원대학교산학협력단 | 미스트 분사 및 급냉식 원자간력 현미경용 시편 처리장치 |
| EP3179229B1 (en) * | 2015-12-11 | 2019-01-30 | FEI Company | Preparation of cryogenic sample for charged-particle microscopy |
| CN110799824B (zh) | 2017-07-07 | 2022-07-08 | 威斯康星校友研究基金会 | 用于冷冻电子显微镜的气相样品制备 |
| EP3495798B1 (en) * | 2017-12-08 | 2021-01-20 | FEI Company | Improved preparation of cryogenic sample, e.g. for charged particle microscopy |
| CN109030519B (zh) * | 2018-06-15 | 2020-11-17 | 义乌市研创工业设计有限公司 | 一种冷冻电镜用的可操作观察记录装置 |
| CN108801732B (zh) * | 2018-06-15 | 2021-01-12 | 李建锋 | 一种低温样品碾碎装置 |
| CN109142399A (zh) * | 2018-07-26 | 2019-01-04 | 聚束科技（北京）有限公司 | 一种成像系统及样品探测方法 |
| US11101104B2 (en) * | 2019-08-30 | 2021-08-24 | Fei Company | Multi modal cryo compatible GUID grid |
| CN110632105B (zh) * | 2019-09-17 | 2021-10-29 | 东南大学 | 一种用于透射电镜表征的液体样品腔及其制备方法 |
| CN111413356A (zh) * | 2020-04-07 | 2020-07-14 | 中国科学院生物物理研究所 | 一种冷冻超薄切片的制备方法 |
| JP2022018555A (ja) * | 2020-07-15 | 2022-01-27 | 学校法人 工学院大学 | 急速凍結装置、及び急速凍結方法 |
| US11728146B2 (en) | 2021-01-13 | 2023-08-15 | Wisconsin Alumni Research Foundation | Retractable ion guide, grid holder, and technology for removal of cryogenic sample from vacuum |
| CN115406914B (zh) * | 2021-05-10 | 2025-04-11 | 中国科学院物理研究所 | 制备附着式冷冻样品的装置及方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3145505A1 (de) * | 1981-11-17 | 1983-05-26 | C. Reichert Optische Werke AG, 1170 Wien | Transfereinrichtung zur entnahme von gefriergetrockneten schnitten aus der kuehlkammer eines gefriermikrotomes, insbesondere eines kryo-ultramikrotomes |
| JPS62156853U (enExample) * | 1986-03-27 | 1987-10-05 | ||
| CA2051092C (en) * | 1990-09-12 | 2002-07-23 | Stephen A. Livesey | Method and apparatus for cryopreparation, dry stabilization and rehydration of biological suspensions |
| US5435850A (en) | 1993-09-17 | 1995-07-25 | Fei Company | Gas injection system |
| JP3727532B2 (ja) * | 2000-12-26 | 2005-12-14 | シャープ株式会社 | 薄片試料処理装置及び薄片試料処理方法 |
| JP2009008657A (ja) * | 2007-05-25 | 2009-01-15 | Canon Inc | 固体試料、固体試料作製方法及び固体試料作製装置 |
| JP4722969B2 (ja) * | 2007-06-29 | 2011-07-13 | エフ イー アイ カンパニ | マニピュレータへのサンプル取付け方法 |
| EP2009420A1 (en) * | 2007-06-29 | 2008-12-31 | FEI Company | Method for attaching a sample to a manipulator |
| US8709269B2 (en) * | 2007-08-22 | 2014-04-29 | Applied Materials Israel, Ltd. | Method and system for imaging a cross section of a specimen |
| JP2010008141A (ja) * | 2008-06-25 | 2010-01-14 | Canon Inc | 固体試料の作製装置、固体試料の作製方法及び試料の観察方法 |
| JP5202136B2 (ja) * | 2008-07-02 | 2013-06-05 | 株式会社日立ハイテクノロジーズ | 荷電粒子線装置 |
| KR20130126684A (ko) * | 2011-03-04 | 2013-11-20 | 가부시키가이샤 히다치 하이테크놀로지즈 | 전자 현미경용 시료 홀더 및 시료 관찰 방법 |
-
2012
- 2012-02-13 EP EP12155112.1A patent/EP2626885A1/en not_active Withdrawn
-
2013
- 2013-02-07 CN CN201310048814.8A patent/CN103247506B/zh active Active
- 2013-02-08 EP EP13154508.9A patent/EP2626886B1/en active Active
- 2013-02-12 JP JP2013024533A patent/JP6143487B2/ja active Active
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