JP2013082747A - Washing liquid for oral cavity - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a drink, a washing liquid, a cosmetic, a medicine, and a quasi drug as a refreshing beverage, and a sport drink, and having functions usable for washing oral cavity, throat and nose.SOLUTION: A functional drink is made by adding salt to tea, coffee, plant extract liquid, or vinegar and blending. A washing liquid, a cosmetic, a medicine, and a quasi drug are made by adding a liquid obtained by adding salt or bittern to tea, coffee, plant extract liquid, or vinegar.

Description

  The present invention relates to an oral cleaning solution characterized by adding salt or bittern to a plant extract.

Conventional tea drinks, coffee drinks, plant-extracted drinks and vinegar drinks are beverages that use water, sweeteners, fruit juices, chemically synthesized food additives, non-chemically synthesized additives, and functional new materials. There is. There is also an extracted beverage using deep ocean water. (For example, refer to Patent Document 1) In addition, there are barley tea beverages having an effect of improving blood flow in which the amounts of magnesium and potassium are adjusted. (For example, refer to Patent Document 2).
Japanese Patent No. 3456855 Japanese Patent Laid-Open No. 2005-151981

  Traditionally, commercially available tea drinks, coffee drinks, plant extract drinks, vinegar drinks, etc., have beverages with adjusted sweetness such as sugar, fructose, sweeteners, etc., but add salt to make them functional. There is no drink.

  Therefore, the present invention has been made paying attention to the conventional problems as described above, and aims to provide an oral cleaning solution for oral cavity, throat and nasal cleaning by combining a plant extract and a salt. To do.

In order to solve the above-mentioned problems, the present invention is an oral cleaning liquid characterized by adding a salt to a plant extract and preparing it.
The salinity at that time is preferably 0.1% to 10%, more preferably 0.1% to 3%, and even more preferably 0.1% to 1.0%. In particular, it is preferable to prepare a sodium chloride concentration almost isotonic with the body fluid, and a salt concentration equivalent to that of physiological saline is preferable.
The salt used may be any of purified salt, rock salt, regenerated salt, and natural sea salt.

The plant extract may be any extract as long as it is extracted from plants used for beverages by various extraction methods.
The target plant may be any edible plant, such as moon peach, herbs (star anise, licorice, mallow blue, linden, rose pink, safflower, blueberry, lemon palm, marjoram, verbita, clove. , Stevia, Rose Red, Wild Strawberry, Penny Royal Mint, Sage, Green Tea, Meadows Sweet, Mallow Black, Eucalyptus, Organo, Hibiscus (Roselle)), etc.

Also, the extraction method may be an existing extraction method, and for example, a pressing method, a steam distillation method, a hot water extraction method, a solvent (alcohol) extraction method, an adsorption method, a cold immersion method (enflurage) and the like can be used.

In Okinawa in this prefecture, salt, herbal tea, and medicinal plants are abundant resources, so they can be manufactured in large quantities at low cost and stably.

In addition, by setting the salinity equivalent to that of physiological saline, the osmotic pressure due to the salinity is reduced, and bacteria attached to the surface of each tissue in the human body are easily washed away by simple washing.

Further, due to the interaction between the antibacterial and bactericidal actions contained in the plant extract and the above-mentioned washing action, the washing effect suitable for oral washing, throat washing, nasal washing, etc. is achieved.

FIG. 1 is a diagram showing a production process of an oral cleaning liquid containing a salt according to the present invention.

T-1) Pure water boiling 4 L of pure water is boiled. Stop heating after boiling.

T-2) 8 g of extracted plant-immersed hibiscus, 2 g of licorice, and 4 g of moon peach were immersed.
The extracted plant is preferably about 0.1 to 10.0% by weight with respect to the extraction solvent.

T-3) Extraction Extraction was performed for 30 minutes. Stir every 10 minutes.

T-4) 24 g of salt was added to the salt-added extract, and the mixture was sufficiently stirred.

T-5) Completely stirred thoroughly to give a new oral cleaning solution.

As a result of tasting the oral cleaning liquid according to the present invention described above, the fragrance of the herb and the salty taste were harmonized, resulting in a cleaning liquid that can be drunk.

The mouthwash was rinsed well with the mouth washing solution according to the present invention, the throat mussel was removed, and the gums were tightened and the refreshing feeling spread in the mouth.

[Test Example 1]

8 kinds of moon peach essential oil components and various herbs were tested for antibacterial activity against pathogenic bacteria and resident bacteria in the oral cavity and intestinal tract.

1) Strain used As the strain, the pathogenic bacteria, the oral resident bacteria and the intestinal resident bacteria shown in FIGS.

2) Moon peach essential oil component Moon peach essential oil component is composed of 8 kinds of components such as α-pinene, 1.8-cineole, p-cytone, borneol, α-terpineol, camphor, methyl cinnamate and thymol shown in FIG. used.

3) Herbs The herbs are shown in Figure 3, Star anise, licorice, mallow blue, linden, rose pink, safflower, blueberry, lemon palm, marjoram, velvet, clove, stevia, rose red, wild strawberry, penny royal mint, Twenty-two kinds of sage, tea, medius sweet, mallow black, eucalyptus, organo, and hibiscus were used.

  The water extract of the herb was prepared by crushing each herb with a mixer, weighing 2 g, adding 20 ml of distilled water thereto, and heating and extracting at 100 ° C. for 30 minutes. After centrifugation at 3.000 × g for 20 minutes, the supernatant was taken and filtered using a membrane filter having a pore size of 0.22 μm, and this was used as a 10% aqueous extract.

4) Antibacterial activity test This test was conducted by two methods.

a) Antibacterial activity test by disc method Prepare bacteria cultured in Mueller Hinton broth (Difco) to 106 cfu / ml with physiological saline and apply to Mueller Hinton agar medium.

  25 μl of various moon peach extracts were dropped onto a disc (diameter 6 mm, Toyo filter paper) previously sterilized by dry heat, and then dried.

  The dried disc was placed on the medium inoculated with the test bacteria, cultured at 37 ° C. overnight, and the diameter of the inhibition circle that appeared around the disc was measured with a caliper.

  Lactobacillus and Streptococcus pneumoniae use 5% sheep blood-added Mueller Hinton medium, Haemophilus influenzae is Hemophilus test medium (Mueller Hinton agar medium, 15 μg / ml NAD + 15 μg / ml bovine hematin + 5 mg / ml yeast extract, pH 7.4 The modified GAM agar medium was used for each anaerobic bacterium.

  Measurement of antibacterial activity other than essential oil components: 20 μl of the test substance was directly dropped onto a plate previously coated with a culture solution and cultured at 37 ° C. A sample in which a clear circle of inhibition was observed after the culture was defined as +.

b) Culture method Streptococcus and Haemophilus influenzae were cultured in candle culture, and anaerobic bacteria were cultured in an anaerobic jar equipped with an Anaeroback at 37 ° C for 48 hours. Others were aerobic cultured.

5) Test results Fig. 2 shows the effect of the moon peach essential oil component on the growth of various microorganisms. Of the eight essential oil components tested, the α-terpineol and thymol components showed the broadest spectrum, followed by α-pinene and 1.8-cineole.

  P-cytone, borneol, camphor and methyl cinnamate did not show growth inhibitory activity against all the strains used this time.

  It was α-terpineol and thymol components that inhibited the growth of oral bacteria, and the remaining 6 essential oil components were not effective.

  In addition to oral bacteria, α-terpineol showed growth inhibitory activity against MRSA, O-157, food poisoning, periodontal bacteria and yeast.

  Thymol was active against many genus of Gram-positive bacteria, Staphylococcus, Streptococcus and Enterobacteriaceae.

  Moreover, FIG. 3 shows the result of having tested about the influence which it has on the growth of various bacteria using 22 types of herbs (10% aqueous extract).

  It shows the antibacterial spectrum of herbs that inhibited the growth of one or more strains, with the broadest spectrum being Hibiscus (Rosel), followed by Mallow Black, Licorice, Rose Pink, Meadows Sweet, and Clove. It was.

  In addition, for the antibacterial activity against three typical bacteria, Shigella (Group B), enterohemorrhagic Escherichia coli, and methicillin-resistant Staphylococcus aureus, the following 11 types including 8 kinds of moon essence essential oil components were tested. 4 shows.

  1, α-pinene 2, cineol 3, P-cytone 4, borneol 5, α-terpineol 6, camphor 7, methyl cinnamate 8, thymol 9, roselle (5% 10 μl) 10, licorice (5%) 11, Moromi black vinegar

6) Evaluation of results Recent research has revealed that the ingredients of moon peach have deodorant, insect-repellent and antibacterial effects, and research on their effective use is thriving.

  As for the bactericidal effect of moon peach, the growth of bacteria such as Staphylococcus aureus, Escherichia coli, Neisseria pneumoniae and Bacillus subtilis is suppressed, and in recent years, antibacterial activity against H. pylori has been reported. In addition, it has already been reported to exhibit antibacterial activity against fungi such as ringworm.

However, there are few studies on oral bacteria, periodontal disease bacteria, and pathogenic bacteria of respiratory infections.
In this study, we investigated the effects of moon peach oil components and various herb components on the growth of periodontal disease bacteria, streptococci resident in the oral cavity, causative bacteria of respiratory infections, and strains causing oral infection.

  Among the moon peach essential oil components, α-terpineol and thymol component showed a strong growth inhibitory action against oral streptococci.

  Of the 22 herbs, mallow black and hibiscus were the only ones that showed oral bacterial growth inhibition.

[Test Example 2]

  Using the oral cleaning liquid produced in the above example, gargle was performed for 1 week, and a saliva test was performed.

1) Test conditions Subject: 36-year-old male Brushing teeth twice a day (morning and evening)
No smoking Oral care: No particular gargle: 3 times a day (morning, noon, evening)
Garage implementation period: 1 week

2) Test results The saliva test was performed using a saliva test kit: dent cult of Oral Care.

  Dent cults are used by many WHO (World Health Organization) epidemiological surveys, as well as many Western universities and public institutions with advanced preventive dentistry, and have been introduced by many universities in Japan. .

a) Measurement of mutans: DentocultSM
A mutans bacterium is a bacterium that creates a trigger for caries and serves as the largest barometer for the occurrence and progression of caries, and identifies mutans bacteria in saliva and plaque by colonies.

b) Measurement of Lactobacillus: Dentocult LB
Lactobacillus is a fungus that promotes dental caries, and tends to increase when carbohydrate intake is high. Therefore, it is possible to infer disorder of eating habits from the test result. Bacteria present in the deep part of the axilla and around the defective prosthesis.

  FIG. 5 shows the measurement results of mutans and lactobacillus in saliva.

(1) shows the result before gargle execution, and (2) shows the measurement result one week after gargle execution.
The left side is the measurement result of Lactobacillus (Dentocult LB), and the right side is the measurement result of D. mutans (Dentocult SM).

Lactobacillus was reduced, but it was about the same 10 ³ , but a remarkable decrease was confirmed for mutans.

[Test Example 3]

  Under the test conditions of Test Example 2 above, halitosis was measured before gargle and after 1 week of gargle.

Test equipment: Hasegawa bad breath measuring instrument Oral chroma Measurement range: H ₂ S, CH ₃ SH, (CH ₃ ) ₂ S

Volatile sulfur compounds (VSCs), which are the main causative components of bad breath, are classified into three gases (hydrogen sulfide: H ₂ S, methyl captan: CH ₃ SH, dimethyl sulfide: (CH ₃ ) ₂ S) Measure.

  The measurement results are shown in FIG. (1) shows the measurement results before gargle execution, and (2) shows the measurement results after 20 minutes of gargle execution.

  It was confirmed that hydrogen sulfide greatly exceeded the cognitive threshold before gargle implementation, whereas it was significantly below the cognitive threshold 20 minutes after gargle implementation.

  It was confirmed that when mouthwashing was performed using the oral cleaning liquid according to the present invention, it was effective in preventing bad breath.

It is a flowchart which shows the manufacture process of the oral cavity cleaning liquid by this invention. It is a figure which shows the antibacterial activity test result of the moon peach essential oil component which affects the growth of various bacteria. It is a figure which shows the antibacterial activity test result of the herb water extract which influences the growth of various microorganisms. It is a figure which shows disk test results, such as a moon peach essential oil component and herbs with respect to Shigella, Escherichia coli, and Staphylococcus aureus. It is a figure which shows the measurement result of the mutans bacterium and Lactobacillus bacterium in saliva. It is a figure which shows a bad breath measurement test result.

Claims (2)

For plant extracts
So that the sodium chloride concentration of the extract is the same as that of physiological saline,
It is an oral cleaning solution to which salt or bittern is added,
An oral cleaning liquid characterized by being able to wash out the oral cavity by washing away bacteria adhering to the oral cavity when drinking it. For plant extracts containing extracts from one or more of moon peach, licorice, roselle,
So that the sodium chloride concentration of the extract is the same as that of physiological saline,
It is an oral cleaning solution to which salt or bittern is added,
An oral cleaning liquid characterized by being able to wash out the oral cavity by washing away bacteria adhering to the oral cavity when drinking it.