JP2013079208A - Prohibitin production promotor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To find ingredients promoting production of prohibitin; and to provide prohibitin production promoters applicable to various skin symptoms, and a skin external preparation including the prohibitin production promoters.SOLUTION: The prohibitin production promoters include extract of eucalyptus and/or turmeric. The skin external preparation includes the prohibitin production promoters.

Description

  TECHNICAL FIELD The present invention relates to a prohibitin production promoter characterized by containing an extract of eucalyptus and / or turmeric, and a skin external preparation containing such a prohibitin production promoter.

Causes of skin symptoms such as aging, disease, stress, wrinkles due to ultraviolet rays, blemishes, reduced skin elasticity, dryness, decreased cellular function, melanin production and pigmentation due to ultraviolet rays, decrease or degeneration of dermal matrix components, ultraviolet rays, etc. Examples include cell oxidative damage. In order to prevent and ameliorate such skin symptoms, various active ingredients have been searched and formulated.

For example, collagen, which is a major component of the dermis, can be cited as a protein and peptide that function functionally on the skin. Collagen is applied and put on the market as a skin external preparation, a functional oral composition, and the like as a highly safe moisturizing agent having an excellent moisturizing action and as an anti-aging agent having an anti-aging and improving action on the skin. As such collagen, marine collagen derived from fish (see Patent Document 1), plant-derived collagen-like protein (see Patent Document 2), and the like are known. Examples of other functional proteins include sericin obtained from persimmon persimmon (see Patent Document 3). Sericin is known as a surfactant having an antioxidant action, a whitening action, and a moisturizing action (see Patent Document 4).

  Prohibitin is a protein localized in the inner mitochondrial membrane. It has been suggested that prohibitin (prohibitin 1) and prohibitin 2 form a complex and are involved in cell cycle regulation, signal transduction of membrane permeability, and control of life span. It has also been implicated in the regulation of transcription, apoptosis, mitochondrial respiratory activity, and cellular senescence. (See Non-Patent Document 1) Further, in nematodes, involvement of hibitin 1 in aging and early development (see Non-Patent Document 2) has been reported. In cultured neonatal cardiomyocytes, when overexpressing prohibitin, oxidation occurs. There is a report that protects mitochondria from injury caused by stress (see Non-Patent Document 3). In addition, there is a possibility that prohibitin is involved in the life span extending action under calorie restriction in rat liver (see Non-Patent Document 4), and in the colon, prohibitin inhibits TNF-α-induced nuclear translocation of NF-κB. It is suggested to inhibit and prevent barrier failure (see Non-Patent Document 5).

Thus, it has been suggested that prohibitin has various functions, and the function is expected to be clarified. However, the physiological actions and effects of prohibitin in human skin cells have not yet been studied in detail, and there are still many unclear points. And no examples have been reported in which prohibitin is used for external preparations for skin, particularly cosmetics.

  Therefore, the present inventors conducted various studies under the hypothesis that prohibitin may exert some advantageous action on the skin. As a result of these studies, the present inventors have found that prohibitin exhibits a moisturizing action, an anti-aging action, a photoaging inhibiting action and a whitening action in the skin.

JP 2003-92997 A JP-A-6-107517 JP-A-4-202435 Japanese Patent Laid-Open No. 11-276876 Kasashima K, et al. J Biol Chem. 2006 Nov 24; 281 (47): 36401-10 Artal-Sanz M, et al. J Biol Chem. 2003 Aug 22; 278 (34): 32091-9 Liu X, et al. Cell Stress Chaperones. 2009 May; 14 (3): 311-9 Takahashi S, et al. Biochem Biophys Res Commun. 2011 Feb 18; 405 (3): 462-7 Theiss A, et al. Mol Biol Cell. 2009 Oct; 20 (20): 4412-23

  Prohibitin has been shown to exhibit moisturizing, anti-aging, photoaging, and whitening effects on the skin. It was also shown that skin symptoms can be prevented or ameliorated by whitening. Thus, the present inventors have found a component that promotes the production of prohibitin, and have made various studies for the purpose of providing a prohibitin production promoter that can be applied to various skin conditions.

  The present inventors examined the effect of promoting the production of prohibitin with respect to various naturally derived components. As a result, the present inventors have found an excellent inhibitory effect on prohibitin production in extracts obtained from eucalyptus and turmeric, and have further studied to complete the present invention.

  That is, the present invention relates to a prohibitin production promoter characterized by containing an extract of eucalyptus and / or turmeric, and an external preparation for skin containing those prohibitin production promoters.

  ADVANTAGE OF THE INVENTION According to this invention, the inhibitor of prohibitin production which exhibits the outstanding effect can be provided by mix | blending the extract of eucalyptus and / or turmeric.

  Eucalyptus globulus used as a raw material of the present invention is an evergreen tree of the genus Eucalyptus. Although it is distributed in the southeastern end of Australia and Tasmania, it is cold-resistant and fast growing, so it is used for afforestation and afforestation throughout the world. Essential oil is collected from the leaves and used for medicinal purposes, insecticides, fragrances and the like. The main component of essential oil is cineole.

  Turmeric (Curcuma Longa) used as a raw material of the present invention is a perennial plant belonging to the genus Turmeric. Widely distributed in tropical Asia, Africa and Australia. Used for spices, dyes, medicinal purposes.

  When using these plants, raw materials or dried products may be used, but it is preferable to use extracts extracted using various solvents. For extraction, any part of the plant such as whole plant, stem, leaf, flower, seed, root, rhizome, fruit, bud, etc. may be used. Leaves and turmeric roots should be used. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days.

  Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as ethyl ether and propyl ether. And solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  Extracts of the above-mentioned solvents of each plant can be used as they are, but the concentrated and dried products can be used again by dissolving them in water or a polar solvent, as long as these physiological functions are not impaired. It may be used after performing purification treatment such as decolorization, deodorization, and desalting, and fractionation treatment by column chromatography. The said extract, its processed material, and a fraction can also be lyophilized | freeze-dried after each process and fractionation, and can also be melt | dissolved and used for a solvent at the time of use.

  The extract of eucalyptus and / or turmeric has an excellent activity for promoting the production of prohibitin, and can be used as a promoter for promoting the production of prohibition.

  Prohibitin production promoter characterized by containing an extract of eucalyptus and / or turmeric can be used alone, but various inhibitors such as pharmaceuticals, quasi-drugs, foods and drinks, cosmetics, etc. can be used as the inhibitor of production of inhibitor By blending with the above composition, it is possible to obtain a composition having an activity of promoting prohibitin production.

  The prohibitin production promoter characterized by containing an extract of eucalyptus and / or turmeric is not limited at all in terms of its form and the presence or absence of other ingredients. As for the form, any form such as liquid, paste, gel, solid, powder, etc. can be selected according to its use and the like, vehicle (excipient), solvent, Other general additives (antioxidants, colorants, dispersants, etc.) can optionally be included.

  The composition containing the inhibitor for promoting the production of prohibitin characterized by containing an extract of eucalyptus and / or turmeric can take any dosage form. When the composition is a skin cosmetic, a hair cosmetic, a detergent, etc., it can be provided as a solubilizing system such as lotion, a dispersion system such as calamine lotion, or an emulsifying system such as cream or emulsion. Furthermore, it can provide with various dosage forms, such as an aerosol form filled with a propellant, an ointment, a poultice.

  Specifically, various cosmetics such as emulsions, creams, lotions, lotions, packs, cosmetic liquids, cleaning agents, makeup cosmetics, etc .; liquids, ointments, powders, granules, aerosols, patches, poultices, etc. Various forms of cosmetics, quasi drugs, topical medicines, and the like can be exemplified.

In the case of oral pharmaceuticals, etc., general dosage forms such as liquid preparations such as drinks and infusions, solid preparations such as gums and candy, capsules, powders, granules and tablets can be used.

  In addition to these, a composition containing a promoter for promoting the production of hibitin characterized by containing an extract of eucalyptus and / or turmeric includes, in addition to its use and necessity, pharmaceuticals, quasi drugs, skin cosmetics Optional ingredients that are usually blended in cosmetics, hair cosmetics, cleaning agents, and oral pharmaceuticals, such as water, oily ingredients, moisturizers, powders, pigments, emulsifiers, solubilizers, gelling agents, detergents , UV absorbers, anti-inflammatory agents, thickeners, pH adjusters, chelating agents, drugs (medicinal ingredients), fragrances, resins, fungicides, fungicides, antioxidants, alcohols, and the like can be appropriately blended. . Furthermore, other moisturizers, anti-aging agents, whitening agents, antioxidants and slimming agents, or various plants / fungi or extracts thereof can be used as long as the effects of the present invention are not impaired.

  The amount of prohibitin production promoter characterized by containing an extract of eucalyptus and / or turmeric can be adjusted according to the type and purpose of the composition, but the effect and stability In view of the above, the content is preferably 0.0001 to 10.0% by mass, more preferably 0.001 to 5.0% by mass, and still more preferably 0.01 to 5.0% by mass with respect to the total amount. It is.

  The test method for evaluating the inhibitory effect on prohibitin production will be described in more detail below, but the technical scope of the present invention is not limited thereto.

[Sample 1]
As the eucalyptus extract, the trade name “Eucalice Extract ET” (manufactured by NOF Corporation) was used.

[Sample 2]
The trade name “Tropical Turmeric Extract BG” (manufactured by NOF Corporation) was used as the turmeric extract.

  Using the above samples, the effect of promoting the production of prohibitin was evaluated.

<CDNA synthesis>
Normal human epidermal keratinocytes NHEK were seeded in a 96-well microplate so as to be 1.0 × 10 ⁴ cells per well. Humedia-KG2 (Kurabo NHEK growth medium) was used as a seeding medium. After culturing for 24 hours, the medium was replaced with KG2 medium supplemented with a sample prepared at an arbitrary concentration, and further cultured for 24 hours. Superscript III cells direct cDNA Synthesis System (Invitrogen kit) was used for mRNA extraction and cDNA synthesis.

<Measurement of expression level of prohibitin mRNA>
Using the cDNA obtained by cDNA synthesis in the previous section as a template, the mRNA expression level of prohibitin was measured by the real-time PCR method. Applied Biosystems 7900HT Fast real-time PCR system (Applied Biosystems Japan) was used as an evaluation instrument. As a reagent, SYBR Premix Ex Taq II (manufactured by Takara Bio Inc.), prohibitin sense-side primer 5′-GTTCTCGACCACCGTAATGTGC-3 ′ and antisense-side primer 5′-AGCACACGCTCATCATAGTCCC-3 ′ or GAPDH sense-side primer 5′CCCTCGCT -3 ′ and antisense primer 5′-CACCCTGTTGCTGTAGCCAA-3 ′ were used. The measured value of prohibitin is corrected with the measured value of GAPDH, and the evaluation results of the inhibitory effect on the expression of prohibitin by the extract treatment are shown as relative values in Tables 1 and 2 with the untreated value of the extract treated as a control.

  As is clear from Table 1 and Table 2, the eucalyptus extract and the turmeric extract were found to have an excellent effect on promoting the production of prohibitin.

Then, although the formulation example of the skin external preparation which mix | blended the prohibitin production promoter is shown, the composition which mix | blends the prohibitin production promoter of this invention is not limited to these. Samples 1 and 2 were used as the inhibitor for promoting the production of prohibitin blended in each formulation example.

[Example 1] Emulsion (1) Squalane 10.0 (mass%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 100 (11) Arginine (1% by weight aqueous solution) 20.0
(12) Prohibitin production promoter 2.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After cooling, (11) and (12) are sequentially added at 40 ° C. and mixed uniformly.

[Example 2] Lotion (1) Ethanol 15.0 (mass%)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 100 (5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Prohibitin production promoter 1.0
Production method: (2) and (3) are dissolved in (1). Further, after sequentially adding (4) to (8), the mixture is sufficiently stirred, and (9) is added and mixed uniformly.

[Example 3] Cream (1) Squalane 10.0 (mass%)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20 mass% aqueous solution) 15.0
(10) Remainder 100 (11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Prohibitin production promoter 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. (11) is added and stirred, then cooled and (12) is added at 40 ° C. and mixed uniformly.

[Example 4] Cosmetic liquid (1) The balance (mass%) of purified water 100
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1% by weight aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (derived from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by mass aqueous solution) 2.0
(16) Prohibitin production promoter 3.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. After cooling, add (15) at 50 ° C. and (16) at 40 ° C. and mix uniformly.

[Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (mass%)
(2) The balance made into purified water 100 (3) Sodium hydroxide (10 mass% aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Prohibitin production promoter 0.5
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 1.0
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Example 6] Cleansing fee (1) Squalane 81.0 (mass%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Prohibitin production promoter 4.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) is added to this, and it mixes uniformly.

[Example 7] Face-wash foam (1) Stearic acid 16.0 (mass%)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 25.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 100 (8) Prohibitin production promoter 0.1
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the water phase components (5) to (7) are heated and dissolved at 80 ° C., and the oil phase components are uniformly mixed and stirred. After cooling, add (8) at 40 ° C. and mix uniformly.

[Example 8] Make-up base cream (1) Squalane 10.2 (% by mass)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) The balance which makes 100 purified water (8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Prohibitin production promoter 3.0
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. After cooling, the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (mass%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Remainder water 100 (11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Prohibitin production promoter 0.5
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the water phase components (7) to (10) are mixed, dissolved by heating at 75 ° C., the pigments (11) to (15) are added thereto, and the mixture is uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. After cooling, components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Example 10] Water-in-oil emollient cream (1) Liquid paraffin 34.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Prohibitin production promoter 3.0
(11) The balance of 100 purified water (12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C, and gradually add to (4) heated to 50 ° C with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. with stirring, and emulsified with a homomixer. After cooling, add (12) at 40 ° C. and mix uniformly.

[Example 11] The balance (mass%) of pack (1) purified water 100
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 9.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Prohibitin production promoter 1.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After evenly dissolving, add (4) and (5) and cool with stirring. Add (6) and (7) at 40 ° C. and mix uniformly.

[Example 12] Bath agent (1) Fragrance 0.3 (mass%)
(2) Prohibitin production promoter 3.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 46.7
Production method: (1) to (4) are mixed uniformly.

[Example 13] Hair wax (1) Stearic acid 3.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) The balance of purified water 100 (11) Prohibitin production promoter 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. After cooling, the components (11) and (12) are added at 40 ° C. and mixed uniformly.

Example 14 Hair artic (1) Ethanol 50.0 (mass%)
(2) The balance of 100 purified water (3) Prohibitin production promoter 3.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

  The external preparation for skin shown in Examples 1 to 14 was a composition having a promoting action on prohibitin production.

Claims (2)

  A preventive production promoter comprising eucalyptus and / or turmeric extract.   An external preparation for skin containing the inhibitor for promoting production of prohibitin according to claim 1.