JP2012501437A - 粒子選別 - Google Patents
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- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
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Abstract
【選択図】図1A
Description
超音波音場が起動するとすぐに、流体は流体−流体界面において音響放射力を受け、その結果、流体の対流運動が起こる。数パーセントの範囲の密度差が、例えばシャッフル流とサンプル流との間の界面にあれば、効果的な流体運動を発生させるのに十分である。流体界面に作用する音響放射力FI、
Claims (14)
- キャビティ(12)と、
前記キャビティ(12)内に入る流体流(14)の入口(16)と、
前記キャビティ(12)から出る前記流体流の第1の部分(22)の第1の出口(18)と、
前記キャビティ(12)から出る前記流体流の第2の部分(24)の第2の出口(20)であって、
それによって、前記流体流(14)は、前記入口(16)と前記第1の出口(18)及び第2の(20)出口との間に流れ方向(X)を有し、
前記第2の出口(20)は、前記第1の出口(18)から、前記流れ方向(X)に対して横向きである第1の方向(Y)にずれている、第2の出口(20)と、
前記キャビティ(12)内の前記流体流(14)の分離ゾーン(36)において音響定常波を印加するように配置された音響発生器(40)であって、前記定常波は、前記流れ方向(X)とともに前記第1の方向(Y)に対して横向きである、音響発生器(40)と、
前記流体流(14)に含まれる粒子(32)に関係する入力データ信号(44)に基づいて前記音響発生器(40)の選択動作を制御するように配置された制御器(42)であって、それによって、前記粒子(32)を選択的に分離して、前記第1の出口(18)又は前記第2の出口(20)内に入れることができる、制御器(42)と、を備える粒子選別セル(10)。 - 請求項1記載の粒子選別セル(10)と、
前記入口(16)に流体的に接続され、前記キャビティ(12)内に連続した層流流体流を与えるために配置された流れ供給器(2)であって、前記流体流(14)は物理特性界面(26)を有し、前記物理特性界面(26)は、法線が、流れ方向(X)に対して垂直に向けられ、前記音響定常波に対して本質的に垂直であり、前記流れ供給器(2)はさらに、粒子(32)を、前記物理特性界面(26)に隣接する前記層流流体流(14)の中に、前記流れ方向(X)に分離された状態で与えるために配置されている、流れ供給器(2)と、
前記流体流(14)の粒子の粒子特性及び個数の少なくとも一方を検出し、前記制御器(42)に、粒子(32)の粒子特性及び個数のうちの前記検出された少なくとも一方に応答して前記入力データ信号(44)を供給するために配置された検出器(34)と、を備える粒子選別システム(1)。 - 前記検出器(34)は前記分離ゾーン(36)の上流に位置し、前記制御器(42)は、前記入力データ信号が関係する粒子が前記分離ゾーン(36)を通るときに、前記検出器(34)がある特定の特性を検出したか、又は前記第1の粒子がある特定の個数間隔内にある場合に、前記音響定常波を印加するために配置されている、請求項2記載の粒子選別システム。
- 前記物理特性界面(26)は密度界面(27)である、請求項3記載の粒子選別システム。
- 前記密度界面(27)の異なる側面上での密度は0.5%超だけ異なる、請求項4記載の粒子選別システム。
- 前記音響発生器(40)は、前記キャビティ(12)の壁内に配置された圧電トランスデューサ(58)を備える、請求項2記載の粒子選別システム。
- 前記キャビティ(12)の断面寸法は、層流を確実にするほどに十分に小さい、請求項2記載の粒子選別システム。
- 前記音響定常波の周波数は1MHzを上回る、請求項2記載の粒子選別システム。
- 連続した層流流体流(14)を与えるステップ(210)であって、前記流体流(14)は物理特性界面(26)を有し、前記物理特性界面(26)は、法線が流れ方向(X)に対して垂直に向けられている、ステップ(210)と、
粒子(32)を、前記物理特性界面(26)に隣接する前記層流流体流(14)の中に、前記流れ方向(X)に分離された状態で与えるステップ(212)と、
前記粒子(32)の第1の粒子と関係する入力データ信号(44)を得るステップ(214)と、
前記入力データ信号(44)に応答して、音響定常波を前記層流流体流(14)の分離ゾーン(36)において、前記第1の粒子が前記分離ゾーン(36)を通るときに、選択的に印加するステップ(216)であって、前記音響定常波は、前記物理特性界面(26)の法線に対して横向きであり、前記流れ方向(X)に垂直であり、前記音響定常波を印加しないときは、前記第1の粒子を分離して第1のフラクション内に入れ、前記音響定常波を印加すると、前記流れ方向(X)に対して横向きである前記第1の粒子の粒子流路を第2のフラクション内にずらすことが起こる、ステップ(216)と、を含む粒子選別のための方法。 - 前記粒子が、ビーズ、生体試料及び細胞からなるリストから選択される、請求項9記載の方法。
- 前記入力データ信号(44)は粒子の粒子特性及び個数の少なくとも一方に関係する、請求項9記載の方法。
- 前記音響定常波の印加(216)を、ある特定の粒子特性が検出されたときに行なう、請求項11記載の方法。
- 前記音響定常波の印加(216)を、ある特定の個数の粒子が前記検出器(34)を通ったときに行なう、請求項11記載の方法。
- 前記物理特性界面(26)は密度界面(27)である、請求項9記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/198,642 | 2008-08-26 | ||
US12/198,642 US8387803B2 (en) | 2008-08-26 | 2008-08-26 | Particle sorting |
PCT/SE2009/050939 WO2010024753A1 (en) | 2008-08-26 | 2009-08-18 | Particle sorting |
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JP2012501437A true JP2012501437A (ja) | 2012-01-19 |
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JP2011524939A Pending JP2012501437A (ja) | 2008-08-26 | 2009-08-18 | 粒子選別 |
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US (1) | US8387803B2 (ja) |
EP (1) | EP2340112A1 (ja) |
JP (1) | JP2012501437A (ja) |
WO (1) | WO2010024753A1 (ja) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2014151260A (ja) * | 2013-02-07 | 2014-08-25 | Ihi Corp | 固液分離方法及び装置 |
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US11701658B2 (en) | 2019-08-09 | 2023-07-18 | President And Fellows Of Harvard College | Systems and methods for microfluidic particle selection, encapsulation, and injection using surface acoustic waves |
Also Published As
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US20120160746A1 (en) | 2012-06-28 |
EP2340112A1 (en) | 2011-07-06 |
WO2010024753A1 (en) | 2010-03-04 |
US8387803B2 (en) | 2013-03-05 |
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