JP2012121874A - Antidiastatic action agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To find a product having an antidiastatic action among natural products having high safety, and to provide an antidiastatic action agent comprising the same as an active ingredient(s).SOLUTION: As the active ingredient(s) of the antidiastatic action agent, one or more kinds of ingredients derived from natural products selected from the group consisting of the extract of a licorice leaf part, the extract of Actinidia polygama, the extract of Punica granatum, the extract of Machilus odoratissima, the extract of Mallotus philippinensis, the extract of Centella asiatica, the Plum extract, the extract of Japanese maple and a pearl protein hydrolyzate are incorporated.

Description

  The present invention relates to an anti-glycation agent.

  Carbohydrates are very important as energy sources in living organisms including humans. However, on the other hand, glycation reaction (glycation) in which carbohydrate reacts with protein is a reaction that non-enzymatically modifies protein with sugar, which causes denaturation of the protein, cross-linking between proteins, etc. To reduce the function.

  The glycation reaction not only causes direct damage by modifying and changing the structure of extracellular matrix constituent proteins such as collagen, but also affects cellular responses by being recognized by receptors with glycated proteins as ligands. Bring. In particular, the effect of the saccharification reaction is serious for diabetic patients whose glucose concentration in the blood is high. It is known that protein glycation contributes to diabetic complications such as diabetic neuropathy, diabetic retinopathy, and diabetic nephropathy. In addition, it is known that the glycation reaction in the blood vessel wall leads to the progression of arteriosclerosis due to the damage of endothelial cells and the accumulation of denatured proteins.

  In addition, extracellular matrix components such as collagen occupy a majority of the dry weight in tissues such as bone and skin. Therefore, for example, when collagen is glycated and abnormally crosslinked, osteoporosis, osteoarthritis and the like occur in bone and cartilage tissue, and elasticity is reduced and dullness due to yellowing occurs in the skin. Furthermore, since abnormally cross-linked collagen or the like is less susceptible to degradation by collagenase or the like, the expression of collagenase or the like is induced, causing problems such as degradation to normal collagen.

  For this reason, if the saccharification reaction can be suppressed in some form, it is expected to be useful for the prevention or treatment of the above-mentioned diseases, that is, diabetic complications, arteriosclerosis, osteoporosis, osteoarthritis and the like. Furthermore, it is expected to be effective in preventing or improving skin elasticity and dullness.

  Conventionally, N-phenacyl thiazolium bromide is known as a compound having an anti-glycation action (Non-patent Document 1). However, this compound has a safety problem and is not suitable as a pharmaceutical or a topical skin preparation. Moreover, as an ingredient derived from a natural product having an anti-glycation action, for example, an extract from mugwort is known (Patent Document 1). However, it is difficult to provide a substance having an anti-glycation action, and there is a strong demand for the development and provision of further new anti-glycation action substances.

JP 2001-122758 A

Vasan S. et al., Nature, July 18, 1996, Vol. 82, No. 6588, p.275-278

  It is an object of the present invention to provide an anti-glycation agent having an anti-glycation action among highly safe natural products and using it as an active ingredient.

  In order to solve the above-mentioned problems, the anti-glycation agent of the present invention includes a licorice leaf extract, a matababi extract, a pomegranate extract, a Mathilus extract, a Kusnohagashi extract, a dextran extract, a ume extract, and a Japanese maple extract. And one or more kinds of natural product-derived components selected from the group consisting of pearl protein hydrolysates as active ingredients.

  According to the present invention, it is possible to provide an anti-glycation agent having an excellent anti-glycation effect and high safety.

Embodiments of the present invention will be described below.
The anti-glycation agent of the present embodiment is extracted from licorice leaf extract, matatabi extract, pomegranate extract, matylus extract, kusunohagashi extract, biloba extract, ume extract, Japanese maple extract and pearl protein hydrolyzate. 1 type or 2 or more types of natural product origin components chosen from the group which consists of are contained as an active ingredient.

  Here, in the present embodiment, the “extract” refers to an extract obtained using the above plant as an extraction raw material, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or these Either a crude product or a purified product is included.

[Manufacture of licorice leaf extract, matababi extract, pomegranate extract, matylus extract, coxnohagashi extract, budgerigar extract, ume extract, Japanese maple extract]
In this embodiment, the extraction raw material used to produce the licorice leaf part extract, the matababi extract, the pomegranate extract, the Mathilus extract, the Coxnohagashi extract, the gram extract, the ume extract, and the Japanese maple extract is licorice. , Matabi (scientific name: Actinidia polygama), Pomegranate (scientific name: Punica granatum), Matilus (scientific name: Machilus odoratissima), Kusunohagasiwa (scientific name: Mallotus philippinensis Mueller-Argoviensis), Sexsou (scientific name: Centella asiaticme Siebold et Zuccarini) and Japanese maple (scientific name: Acer palmatum).

  Licorice is a perennial herb belonging to the genus Glycyrrhiza. Licorice includes Glychyrrhiza glabra, Glychyrrhiza inflata, Glychyrrhiza uralensis, Glychyrrhiza aspera, Glychyrrhiza aspera, Glychyrrhiza aspera, Glychyrrhiza aspera, Glychyrrhiza aspera, Glychyrrhiza aspera, Glychyrrhiza aspera ), Glychyrrhiza yunnanensis, Glychyrrhiza lepidota, Glychyrrhiza echinata, Glychyrrhiza echinata, Glychyrrhiza acanthocarpa, etc. Licorice may be used as an extraction raw material, especially Glychyrrhiza glabra, Glychyrrhiza uralen sis) and Glychyrrhiza inflata are preferably used as raw materials for extraction. A constituent part of licorice that can be used as an extraction raw material is a leaf.

  Matabi (Actinidia polygama) is a semi-generic plant belonging to the genus Matababi, which is distributed in the Korean Peninsula, China, Japan, etc., and can be easily obtained from these regions. Examples of the constituent parts of matabi which can be used as an extraction raw material include, for example, flower part, bud part, fruit part, fruit skin part, seed part, seed coat part, stem part, leaf part, branch part, branch leaf part, trunk part, bark part, A root part, a rhizome part, a root bark part or a mixture thereof may be mentioned, and a fruit part is preferable.

  Pomegranate (Punica granatum) is a deciduous small shrub belonging to the genus Pomegranate, which is distributed in Iran, Afghanistan, Western Pakistan, etc., and can be easily obtained from these regions. Examples of pomegranate constituent parts that can be used as the raw material for extraction include leaves, branches, bark parts, trunks, stems, fruit parts, fruit skin parts, flower parts, above-ground parts, root parts, or a mixture of these parts. Although it is mentioned, Preferably it is a skin part.

  Machilus (Machilus odoratissima) is an evergreen tree belonging to the genus Machilus, and is distributed in Yunnan, Guizhou, Sichuan, Tibet, etc., and can be easily obtained from these areas. Examples of the constituent parts of Mathils that can be used as an extraction raw material include bark parts, leaf parts, trunk parts, above-ground parts, flower parts, fruit parts, seed parts, root parts, whole grasses, or a mixture of these parts. The bark portion is preferable.

  Kusunohagashi (Mallotus philippinensis Mueller-Argoviensis, Chinese name: Luzon Xuumou) is an evergreen small tree belonging to the genus Aceraceae, Guangdong, Guangxi, Hunan, Yunnan, Sichuan, Zhejiang, Fujian, Jiangxi, It is distributed in Taiwan and can be easily obtained from these areas. Examples of the constituent parts of Kusunohagashi that can be used as an extraction raw material include leaf parts, flower parts, root parts, bark parts, branch parts, or a mixture of these parts, but bark parts are preferred.

  Betula (Centella asiatica) is a perennial that belongs to the genus Button, and is distributed in the southwestern Honshu, Shikoku, Kyushu, Okinawa, Korean Peninsula, Taiwan, China, etc., and can be easily obtained from these areas Can do. Examples of the constituent parts of dextran that can be used as an extraction raw material include a flower part, a root part, a leaf part, a stem part, a branch part, and the like, and preferably a leaf part or a stem part.

  Plum (Prunus mume Siebold et Zuccarini) is a deciduous small tree belonging to the family Rosaceae and is planted for ornamental and edible purposes. It is native to central China, and has come to Japan since ancient times and has become wild in Kyushu. Examples of constituent parts of ume that can be used as an extraction raw material include leaves, branches, bark, trunks, stems, fruit parts, fruit skin parts, flower parts, above-ground parts, root parts, or a mixture of these parts. Although it is mentioned, Preferably it is a fruit part.

  Acer palmatum (scientific name: Acer palmatum) is a deciduous sub-high tree belonging to the genus Acer palmaceae, and is also referred to as Acer palmatum, Acer palmatum, Aca palmae and the like. Japanese maple is distributed in Shikoku, Kyushu, southern Korea, eastern China, etc., and can be easily obtained from these regions. Examples of the constituent parts of Acer palmatum that can be used as an extraction raw material include a leaf part, a branch part, a root part, a bark part, a seed part, or a mixture of these parts, and a leaf part is preferable.

  Details of the anti-glycation substances contained in each of the licorice leaf extract, matata extract, pomegranate extract, matylus extract, coxnohagashi extract, biloba extract, ume extract, and maple extract are unknown. However, an extract having this action can be obtained from the above plant by an extraction method generally used for the extraction of the plant.

  For example, it can be obtained by drying the above plant, pulverizing it as it is or using a crusher, and subjecting it to extraction with an extraction solvent. Drying may be performed in the sun or using a commonly used dryer. Moreover, after performing pretreatment, such as degreasing, with a nonpolar solvent such as hexane, it may be used as an extraction raw material. By performing pretreatment such as degreasing, extraction treatment with the polar solvent of the plant can be efficiently performed.

  As the extraction solvent, it is preferable to use a polar solvent, and examples thereof include water and hydrophilic organic solvents. These are used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. It is preferable.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, and those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, the water that can be used as the extraction solvent in this embodiment includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of hydrophilic organic solvents that can be used as extraction solvents include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when using a mixed solution of water and a lower aliphatic alcohol, it is preferable to mix 1 to 90 parts by volume of a lower aliphatic alcohol with respect to 10 parts by volume of water. When using a mixed solution, it is preferable to mix 1 to 40 parts by volume of a lower aliphatic ketone with 10 parts by volume of water, and when using a mixed solution of water and a polyhydric alcohol, water 10 It is preferable to mix 10 to 90 parts by volume of a polyhydric alcohol with respect to the volume part.

  The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted in the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent 5 to 15 times (mass ratio) of the extraction raw material, the soluble components are extracted at room temperature or under reflux, and then filtered to remove the extraction residue. A liquid can be obtained. When the solvent is distilled off from the obtained extract, a paste-like concentrate is obtained, and when this concentrate is further dried, a dried product is obtained.

  In addition, although the extract obtained as mentioned above can be used as an active ingredient of an anti-glycation agent as it is, a concentrated solution or a dried product is easier to use.

  In addition, since the extract from the plant has a peculiar odor, it can be purified for the purpose of decolorization, deodorization and the like within a range not causing a decrease in its physiological activity. Since it is not used in a large amount when it is blended, etc., there is no practical problem even if it is not purified.

[Production of pearl protein hydrolyzate]
The pearl protein hydrolyzate in this embodiment is obtained by subjecting shells and / or pearls of a shell having a pearl layer to decalcification, and hydrolyzing the pearl protein (conchiolin) obtained thereby.

  Examples of shells having a pearl layer include pearl oysters (scientific name: Pinctada fucata); mussels (scientific name: Mytilus coruscum), mussels (scientific name: Mytilus edulis); (Scientific name: Hyriopsis schlegelii), crow mussel (scientific name: Cristaria plicata), etc. Among them, it is preferable to use pearl oysters.

  Shells and / or pearls of shells having a pearl layer are subjected to a decalcification treatment as they are or after being pulverized. What is necessary is just to perform the decalcification process of a shell and / or a pearl by a conventional method, for example, adding an acid or its aqueous solution to a shell and / or a pearl, and stirring.

  The acid that can be used for the decalcification treatment is not particularly limited as long as it can sufficiently elute inorganic components such as calcium carbonate contained in shells and / or pearls. For example, hydrochloric acid, sulfuric acid, Examples include inorganic acids such as phosphoric acid and nitric acid; and organic acids such as oxalic acid, carbonic acid, citric acid, malic acid, maleic acid, tartaric acid, acetic acid, and formic acid. Alternatively, two or more mixed acids may be used.

  The addition amount of the acid or an aqueous solution thereof may be an amount that can sufficiently elute inorganic components such as calcium carbonate contained in shells and / or pearls, and may be appropriately set according to the type of acid to be used.

  The treatment liquid obtained by the decalcification treatment is subjected to solid-liquid separation means such as centrifugation, filtration, and decantation to collect insoluble matter, and if necessary, purified water is added to the collected insoluble matter and centrifuged again. Repeat washing operations such as separation and filtration to recover insoluble matter. The obtained insoluble matter can be finely pulverized using a pulverizer or the like, if desired, and dried by a conventional method to obtain a pearl protein (conchiolin).

  The obtained pearl protein (conchiolin) is hydrolyzed using an aqueous solution of acid or alkali.

  Examples of the acid that can be used for the hydrolysis treatment of pearl protein (conchiolin) include hydrochloric acid, sulfuric acid and the like. One of these may be used alone, or both may be used in combination. May be.

  Moreover, as an alkali which can be used for the hydrolysis process of a pearl protein (conchiolin), sodium hydroxide, potassium hydroxide, etc. are mentioned, for example, One of these may be used independently or both. May be used in combination.

  When hydrolyzing using an acid or alkali aqueous solution, the amount of acid or alkali aqueous solution added or the acid or alkali concentration in the aqueous solution hydrolyzes pearl protein (conchiolin) contained in shells and / or pearls. However, the amount of the aqueous solution added or the concentration of the acid or alkali in the aqueous solution can be appropriately changed depending on the type of acid or alkali used. .

  The hydrolysis treatment is performed at a temperature of 0 to 130 ° C., preferably 50 to 110 ° C., for about 30 minutes to 10 days, preferably about 5 hours to 5 days.

  In this way, acid or alkali is removed from the solution obtained by the hydrolysis treatment, and the polymer peptide is removed by subjecting to ultrafiltration treatment as necessary, followed by treatment such as concentration and drying. Thus, a pearl protein hydrolyzate can be obtained.

  The acid or alkali can be removed from the solution obtained by the hydrolysis treatment by a conventional method, and examples thereof include freeze drying, a method of neutralizing the acid or alkali in the solution and desalting.

[Anti-glycation agent]
Since each natural product-derived component obtained as described above has an excellent anti-glycation action, it can be used as an active ingredient of an anti-glycation action agent.

  Licorice leaf extract, matababi extract, pomegranate extract, matylus extract, coxnohagashi extract, budgerigar extract, ume extract, Japanese maple extract or pearl protein hydrolyzate, through its anti-glycation action, diabetic nerve It can be used as an active ingredient in prophylactic / therapeutic agents for diabetic complications such as disorders, diabetic retinopathy, diabetic nephropathy, and as an active ingredient for prophylactic / therapeutic agents for arteriosclerosis caused by protein saccharification Can be used. However, licorice leaf extract, matababi extract, pomegranate extract, matylus extract, coxnohagashi extract, biloba extract, ume extract, Japanese maple extract, or pearl protein hydrolyzate is not suitable for these uses. For example, it can be used as an active ingredient of a preventive / ameliorating agent for reducing skin elasticity or dullness caused by a protein saccharification reaction. Furthermore, licorice leaf extract, matababi extract, pomegranate extract, matylus extract, coxnohagashi extract, biloba extract, ume extract, Japanese maple extract or pearl protein hydrolyzate is caused by protein saccharification reaction It can also be used as an active ingredient of a prophylactic / therapeutic agent for diseases such as osteoporosis and osteoarthritis.

  In the present embodiment, among the licorice leaf extract, matababi extract, pomegranate extract, matylus extract, coxnohagashi extract, biloba extract, ume extract, Japanese maple extract or pearl protein hydrolyzate Any one of them may be used as the active ingredient, or these may be mixed and used as the active ingredient. When using licorice leaf part extract, matababi extract, pomegranate extract, matylus extract, coxnohagashi extract, biloba extract, ume extract, Japanese maple extract or pearl protein hydrolysate as the active ingredient, The compounding ratio is that of the anti-glycation action of licorice leaf extract, matatabi extract, pomegranate extract, matylus extract, camphora serrata extract, budgerigar extract, ume extract, Japanese maple extract or pearl protein hydrolyzate. What is necessary is just to adjust suitably according to a grade.

  The anti-glycation agent of this embodiment includes a licorice leaf extract, a matahabi extract, a pomegranate extract, a Mathilus extract, a Kusunohagashi extract, a biloba extract, a ume extract, a Japanese maple extract or a pearl protein hydrolyzate and It may be composed of only these mixtures, licorice leaf extract, matababi extract, pomegranate extract, matylus extract, camphor serrata extract, biloba extract, ume extract, Japanese maple extract or pearl protein hydrolyzate And a mixture of these.

  The anti-glycation agent of this embodiment is an arbitrary pharmaceutically acceptable carrier such as dextrin or cyclodextrin, and any other auxiliary agent such as powder, granule, tablet, liquid, etc. according to a conventional method. It can be formulated into a dosage form. In this case, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring / flavoring agent, and the like can be used. The anti-glycation agent can be used by being blended with other compositions (for example, skin external preparations, cosmetic foods and drinks), and can also be used as an ointment, an external liquid, a patch and the like.

  When the anti-glycation agent of the present embodiment is formulated, a licorice leaf extract, a matababi extract, a pomegranate extract, a Matylus extract, a Kusunohagashi extract, a biloba extract, a plum extract, a Japanese maple extract, or a pearl protein Content of a hydrolyzate and these mixtures is not specifically limited, According to the objective, it can set suitably.

  In addition, the anti-glycation agent of the present embodiment, if necessary, other natural extracts having an anti-glycation action, such as licorice leaf extract, matababi extract, pomegranate extract, matylus extract, Kusunohagashi extract It can be used as an active ingredient by blending with a product, an extract of pebbles, a plum extract, a white maple extract or a pearl protein hydrolyzate and a mixture thereof.

  Examples of the method for administering the anti-glycation agent of the present embodiment to a patient include transdermal administration, oral administration, and the like, and a suitable method for its prevention / treatment may be appropriately selected depending on the type of the disease. .

  In addition, the dose of the anti-glycation agent of the present embodiment may be appropriately increased or decreased depending on the type of disease, severity, individual differences among patients, administration method, administration period, and the like.

  The anti-glycation agent of the present embodiment includes a licorice leaf extract, a matababi extract, a pomegranate extract, a Mathilus extract, a Kusnohagashi extract, a biloba extract, a ume extract, a Japanese maple extract, or a pearl protein hydrolyzate. Through its anti-glycation action, it can prevent or treat diabetic complications such as diabetic neuropathy, diabetic retinopathy, diabetic nephropathy, and also prevent or treat arteriosclerosis caused by protein glycation reaction be able to. In addition, the anti-glycation agent of this embodiment is a licorice leaf part extract, a matababi extract, a pomegranate extract, a Matylus extract, a Kusunohagashi extract, a biloba extract, a ume extract, a Japanese maple extract or a pearl protein hydrolyzate Through the anti-glycation action of the product, it is possible to prevent or improve skin elasticity reduction, dullness, and the like caused by protein glycation reaction. Furthermore, the anti-glycation agent of the present embodiment can also prevent or treat diseases such as osteoporosis and osteoarthritis caused by protein glycation reaction. However, the anti-glycation agent of the present embodiment can be used for all uses other than these uses that are meaningful for exerting an anti-glycation action.

  The anti-glycation agent of the present embodiment is preferably applied to humans, but as long as the respective effects are exhibited, animals other than humans (for example, mice, rats, hamsters, dogs) , Cats, cows, pigs, monkeys, etc.).

  The anti-glycation agent of the present embodiment has an excellent anti-glycation effect and is excellent in usability and safety when applied to the skin.

  Here, the external skin preparation is not limited in its category, and includes a wide range of skin cosmetics, quasi-drugs, pharmaceuticals, etc. Specifically, for example, ointments, creams, milky lotions, cosmetic liquids, Lotions, packs, foundations, lip balms, bath salts, hair nicks, hair lotions, soaps, body shampoos and the like.

  In addition, the anti-glycation agent of the present embodiment has an excellent anti-glycation action and is also excellent in safety when taken orally. is there. Here, there is no restriction | limiting in the classification | category as food / beverage products for beauty, and it includes a wide range of foods such as general foods, health foods, health functional foods, quasi drugs, and pharmaceuticals that are taken orally.

  Furthermore, since the anti-glycation agent of this embodiment has an excellent anti-glycation action, it can be suitably used as a reagent for studying diseases related to saccharification reactions.

  Hereinafter, although a test example is given and this invention is demonstrated further in detail, this invention is not limited to the following test example at all. In this test example, freeze-dried products shown in Table 1 were used as samples (samples 1 to 9).

[Test Example 1] Anti-glycation test The sugar such as glucose is an α-dicarbonyl compound having two consecutive carbonyl groups in the molecule, such as methylglyoxal or 3-deoxyglucosone, due to glycolysis or oxidation. Generate. These α-dicarbonyl compounds are about 10,000 times more reactive than normal sugars, and easily react with amino groups in proteins to produce glycated proteins. Therefore, it is thought that the component which has the effect | action which decomposes | disassembles an alpha-dicarbonyl compound suppresses glycation of protein.

  In this test, 1-phenyl-1,2-propanedione was used to detect the decomposition action of the α-dicarbonyl compound. 1-phenyl-1,2-propanedione has an α-diketone (α-dicarbonyl) structure in the molecule, and when the C—C bond of the α-diketone is cleaved, 1-phenyl-1,2- Benzoic acid is produced in an amount equal to that of propanedione.

  About each said natural product origin component (samples 1-9), the production amount of benzoic acid was used as the parameter | index, and the anti-glycation effect was tested as follows.

  22 mM 1-phenyl-1,2-propanedione (manufactured by Wako Pure Chemical Industries, Ltd.) 2 mL of methanol solution, test samples dissolved in purified water so that the final concentration is 10% (samples 1-9, sample concentration is 1 mL) and 1 mL of 0.1 M phosphate buffer (pH 7.4) were mixed and reacted at 37 ° C. for 10 hours.

  After completion of the reaction, the reaction solution was centrifuged (450 × g, 3 minutes), the supernatant was diluted 10-fold with the mobile phase shown below, and then filtered through a 0.45 μm membrane filter under the following conditions. The amount of benzoic acid produced was quantified by high performance liquid chromatography. As a standard substance, 20 mg of benzoic acid (manufactured by Wako Pure Chemical Industries, Ltd.) was dissolved in the following mobile phase, accurately made up to 100 mL, and filtered through a 0.45 μm membrane filter.

<Liquid chromatography conditions>
Stationary phase: JAIGEL GS-310 (manufactured by Nippon Analytical Industrial Co., Ltd.)
Column diameter: 20mm
Column length: 250mm
Column temperature: 40 ° C
Injection volume: 20 μL
Mobile phase: acetonitrile: phosphoric acid aqueous solution (pH 2.5) = 3: 7 (volume ratio)
Mobile phase flow rate: 1 mL / min
Detection wavelength: 254 nm

  From the obtained results, the anti-glycation action rate (%) was calculated by the following formula.

Anti-glycation action rate (%) = A / B × 100
In the formula, A represents “substance amount of benzoic acid produced” and B represents “substance amount of 1-phenyl-1,2-propanedione added to the reaction solution”.
The results are shown in Table 2.

  As shown in Table 2, since samples 1 to 9 all produced benzoic acid, the extract of licorice leaf part, extract of matababi, pomegranate extract, extract of Matylus, extract of Kusunohagashi, extract of sesame, ume The extract, Japanese maple extract and pearl protein hydrolyzate were found to have anti-glycation action. In particular, it was shown that the Matylus extract (Sample 4), Kusunohagashi extract (Sample 5), Ume extract (Sample 7), and Japanese maple extract (Sample 9) have very excellent anti-glycation action.

  The anti-glycation agent of the present invention is used for the prevention or treatment of diabetic complications such as diabetic neuropathy, diabetic retinopathy, diabetic nephropathy, prevention or treatment of arteriosclerosis caused by protein glycation reaction, protein glycation It can greatly contribute to the prevention or improvement of skin elasticity reduction and dullness caused by the reaction, and the prevention or treatment of diseases such as osteoporosis and osteoarthritis caused by the protein glycation reaction.

Claims (1)

  One or two kinds selected from the group consisting of licorice leaf extract, matababi extract, pomegranate extract, matylus extract, coxnohagashi extract, budgerigar extract, ume extract, Japanese maple extract and pearl protein hydrolyzate An anti-glycation agent containing the above natural product-derived component as an active ingredient.