JP2011522547A5 - - Google Patents

Info

Publication number

JP2011522547A5

JP2011522547A5 JP2011512807A JP2011512807A JP2011522547A5 JP 2011522547 A5 JP2011522547 A5 JP 2011522547A5 JP 2011512807 A JP2011512807 A JP 2011512807A JP 2011512807 A JP2011512807 A JP 2011512807A JP 2011522547 A5 JP2011522547 A5 JP 2011522547A5

Authority

JP

Japan

Prior art keywords

astaxanthin

nucleotide sequence

carotene

beta

production

Prior art date

2009-06-02

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

Links

• Espacenet
• Global Dossier
• Discuss

• 108020004414 DNA Proteins 0.000 claims description 19
• JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 claims description 18
• 235000013793 astaxanthin Nutrition 0.000 claims description 18
• 239000001168 astaxanthin Substances 0.000 claims description 18
• MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 claims description 18
• 229940022405 astaxanthin Drugs 0.000 claims description 18
• 239000002773 nucleotide Substances 0.000 claims description 15
• 125000003729 nucleotide group Chemical group 0.000 claims description 15
• OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 claims description 12
• 108010045510 NADPH-Ferrihemoprotein Reductase Proteins 0.000 claims description 11
• OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 claims description 11
• 235000013734 beta-carotene Nutrition 0.000 claims description 11
• 239000011648 beta-carotene Substances 0.000 claims description 11
• TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 claims description 11
• 229960002747 betacarotene Drugs 0.000 claims description 11
• 238000000034 method Methods 0.000 claims description 10
• 102000004190 Enzymes Human genes 0.000 claims description 6
• 108090000790 Enzymes Proteins 0.000 claims description 6
• 238000006243 chemical reaction Methods 0.000 claims description 6
• 108091034117 Oligonucleotide Proteins 0.000 claims description 5
• 239000012528 membrane Substances 0.000 claims description 5
• 239000013612 plasmid Substances 0.000 claims description 5
• 230000000295 complement effect Effects 0.000 claims description 4
• 150000001413 amino acids Chemical class 0.000 claims description 2
• 230000015572 biosynthetic process Effects 0.000 claims description 2
• 238000003786 synthesis reaction Methods 0.000 claims description 2
• 238000004519 manufacturing process Methods 0.000 claims 11
• 229920001184 polypeptide Polymers 0.000 claims 7
• 102000004196 processed proteins & peptides Human genes 0.000 claims 7
• 108090000765 processed proteins & peptides Proteins 0.000 claims 7
• 125000003275 alpha amino acid group Chemical group 0.000 claims 5
• 230000000694 effects Effects 0.000 claims 5
• 239000000203 mixture Substances 0.000 claims 5
• 230000002068 genetic effect Effects 0.000 claims 2
• 239000000823 artificial membrane Substances 0.000 claims 1
• 238000012258 culturing Methods 0.000 claims 1
• 239000002502 liposome Substances 0.000 claims 1
• 210000001589 microsome Anatomy 0.000 claims 1
• 210000001700 mitochondrial membrane Anatomy 0.000 claims 1
• 238000001823 molecular biology technique Methods 0.000 claims 1
• 239000011541 reaction mixture Substances 0.000 claims 1
• 108090000623 proteins and genes Proteins 0.000 description 28
• 239000012634 fragment Substances 0.000 description 8
• 238000003752 polymerase chain reaction Methods 0.000 description 7
• 230000003321 amplification Effects 0.000 description 5
• 238000003199 nucleic acid amplification method Methods 0.000 description 5
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
• 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
• 238000004925 denaturation Methods 0.000 description 4
• 230000036425 denaturation Effects 0.000 description 4
• 108091028043 Nucleic acid sequence Proteins 0.000 description 3
• 239000011543 agarose gel Substances 0.000 description 3
• 238000004458 analytical method Methods 0.000 description 3
• 238000012217 deletion Methods 0.000 description 3
• 230000037430 deletion Effects 0.000 description 3
• 108020004635 Complementary DNA Proteins 0.000 description 2
• 241000588724 Escherichia coli Species 0.000 description 2
• GRRNUXAQVGOGFE-UHFFFAOYSA-N Hygromycin-B Natural products OC1C(NC)CC(N)C(O)C1OC1C2OC3(C(C(O)C(O)C(C(N)CO)O3)O)OC2C(O)C(CO)O1 GRRNUXAQVGOGFE-UHFFFAOYSA-N 0.000 description 2
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
• 238000000137 annealing Methods 0.000 description 2
• 230000006696 biosynthetic metabolic pathway Effects 0.000 description 2
• 238000010804 cDNA synthesis Methods 0.000 description 2
• 239000002299 complementary DNA Substances 0.000 description 2
• 230000029087 digestion Effects 0.000 description 2
• 239000012153 distilled water Substances 0.000 description 2
• 238000009396 hybridization Methods 0.000 description 2
• GRRNUXAQVGOGFE-NZSRVPFOSA-N hygromycin B Chemical compound O[C@@H]1[C@@H](NC)C[C@@H](N)[C@H](O)[C@H]1O[C@H]1[C@H]2O[C@@]3([C@@H]([C@@H](O)[C@@H](O)[C@@H](C(N)CO)O3)O)O[C@H]2[C@@H](O)[C@@H](CO)O1 GRRNUXAQVGOGFE-NZSRVPFOSA-N 0.000 description 2
• 229940097277 hygromycin b Drugs 0.000 description 2
• 239000013642 negative control Substances 0.000 description 2
• 108091008146 restriction endonucleases Proteins 0.000 description 2
• 239000000523 sample Substances 0.000 description 2
• 230000009466 transformation Effects 0.000 description 2
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
• 108700028369 Alleles Proteins 0.000 description 1
• 241000123346 Chrysosporium Species 0.000 description 1
• 241001290628 Cunninghamella echinulata Species 0.000 description 1
• 241001149409 Cystobasidium minutum Species 0.000 description 1
• 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 description 1
• 108010042407 Endonucleases Proteins 0.000 description 1
• 102000004533 Endonucleases Human genes 0.000 description 1
• YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
• 241000206602 Eukaryota Species 0.000 description 1
• 101150064643 GPD gene Proteins 0.000 description 1
• 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
• 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
• 101710198130 NADPH-cytochrome P450 reductase Proteins 0.000 description 1
• 239000004677 Nylon Substances 0.000 description 1
• 108090000854 Oxidoreductases Proteins 0.000 description 1
• 102000004316 Oxidoreductases Human genes 0.000 description 1
• 101150053185 P450 gene Proteins 0.000 description 1
• 238000012408 PCR amplification Methods 0.000 description 1
• 102000010292 Peptide Elongation Factor 1 Human genes 0.000 description 1
• 108010077524 Peptide Elongation Factor 1 Proteins 0.000 description 1
• 229940127593 SEQ-9 Drugs 0.000 description 1
• 241000235343 Saccharomycetales Species 0.000 description 1
• 101150078565 TEF gene Proteins 0.000 description 1
• 241000222355 Trametes versicolor Species 0.000 description 1
• 239000003242 anti bacterial agent Substances 0.000 description 1
• 229940088710 antibiotic agent Drugs 0.000 description 1
• QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
• 230000003115 biocidal effect Effects 0.000 description 1
• 238000010276 construction Methods 0.000 description 1
• 238000002474 experimental method Methods 0.000 description 1
• 230000006801 homologous recombination Effects 0.000 description 1
• 238000002744 homologous recombination Methods 0.000 description 1
• 238000003780 insertion Methods 0.000 description 1
• 230000037431 insertion Effects 0.000 description 1
• 229920001778 nylon Polymers 0.000 description 1
• 229910052760 oxygen Inorganic materials 0.000 description 1
• 239000001301 oxygen Substances 0.000 description 1
• 238000002360 preparation method Methods 0.000 description 1
• 102000004169 proteins and genes Human genes 0.000 description 1
• 238000010561 standard procedure Methods 0.000 description 1
• 239000000758 substrate Substances 0.000 description 1
• 238000012360 testing method Methods 0.000 description 1
• 238000013519 translation Methods 0.000 description 1