JP2011520467A - Ｄｎａおよびｒｎａ検出のためにサンプルを収集および処理するための方法、キットならびにシステム - Google Patents
Ｄｎａおよびｒｎａ検出のためにサンプルを収集および処理するための方法、キットならびにシステム Download PDFInfo
- Publication number
- JP2011520467A JP2011520467A JP2011510628A JP2011510628A JP2011520467A JP 2011520467 A JP2011520467 A JP 2011520467A JP 2011510628 A JP2011510628 A JP 2011510628A JP 2011510628 A JP2011510628 A JP 2011510628A JP 2011520467 A JP2011520467 A JP 2011520467A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- detection
- nucleic acid
- container
- pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 149
- 238000000034 method Methods 0.000 title claims abstract description 96
- 238000012545 processing Methods 0.000 title claims abstract description 15
- 239000000463 material Substances 0.000 claims abstract description 33
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 28
- 238000011084 recovery Methods 0.000 claims abstract description 27
- 239000000523 sample Substances 0.000 claims description 164
- 150000007523 nucleic acids Chemical class 0.000 claims description 104
- 108020004707 nucleic acids Proteins 0.000 claims description 101
- 102000039446 nucleic acids Human genes 0.000 claims description 101
- 239000003153 chemical reaction reagent Substances 0.000 claims description 49
- 241000238557 Decapoda Species 0.000 claims description 47
- 239000000284 extract Substances 0.000 claims description 43
- 238000003757 reverse transcription PCR Methods 0.000 claims description 29
- 241000700605 Viruses Species 0.000 claims description 23
- 239000007788 liquid Substances 0.000 claims description 23
- 238000001914 filtration Methods 0.000 claims description 22
- 244000052769 pathogen Species 0.000 claims description 18
- 238000009396 hybridization Methods 0.000 claims description 17
- 238000011901 isothermal amplification Methods 0.000 claims description 14
- 239000002245 particle Substances 0.000 claims description 14
- 238000012163 sequencing technique Methods 0.000 claims description 14
- 238000004458 analytical method Methods 0.000 claims description 13
- 239000000243 solution Substances 0.000 claims description 13
- 102000004190 Enzymes Human genes 0.000 claims description 12
- 108090000790 Enzymes Proteins 0.000 claims description 12
- 239000002773 nucleotide Substances 0.000 claims description 11
- 125000003729 nucleotide group Chemical group 0.000 claims description 11
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 10
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 9
- 230000000295 complement effect Effects 0.000 claims description 9
- 238000001917 fluorescence detection Methods 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- 102100034343 Integrase Human genes 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 8
- 230000009089 cytolysis Effects 0.000 claims description 7
- -1 polypropylene Polymers 0.000 claims description 7
- 239000004094 surface-active agent Substances 0.000 claims description 7
- 238000003745 diagnosis Methods 0.000 claims description 6
- 239000003161 ribonuclease inhibitor Substances 0.000 claims description 6
- 239000001103 potassium chloride Substances 0.000 claims description 5
- 235000011164 potassium chloride Nutrition 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 4
- 206010047642 Vitiligo Diseases 0.000 claims description 4
- 208000015181 infectious disease Diseases 0.000 claims description 4
- 230000002458 infectious effect Effects 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 229920001155 polypropylene Polymers 0.000 claims description 4
- 238000005251 capillar electrophoresis Methods 0.000 claims description 3
- 238000003936 denaturing gel electrophoresis Methods 0.000 claims description 3
- 238000001502 gel electrophoresis Methods 0.000 claims description 3
- 239000011544 gradient gel Substances 0.000 claims description 3
- 230000003394 haemopoietic effect Effects 0.000 claims description 3
- 230000017074 necrotic cell death Effects 0.000 claims description 3
- 238000007920 subcutaneous administration Methods 0.000 claims description 3
- 238000000123 temperature gradient gel electrophoresis Methods 0.000 claims description 3
- 239000004698 Polyethylene Substances 0.000 claims description 2
- 239000007853 buffer solution Substances 0.000 claims description 2
- 230000002879 macerating effect Effects 0.000 claims description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 2
- 229920000573 polyethylene Polymers 0.000 claims description 2
- 241000607598 Vibrio Species 0.000 claims 2
- 241001265687 Taura syndrome virus Species 0.000 claims 1
- 239000011343 solid material Substances 0.000 claims 1
- 230000001225 therapeutic effect Effects 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 239000013615 primer Substances 0.000 description 73
- 108020004414 DNA Proteins 0.000 description 52
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 39
- 210000001519 tissue Anatomy 0.000 description 39
- 238000003752 polymerase chain reaction Methods 0.000 description 31
- 230000003321 amplification Effects 0.000 description 28
- 238000003199 nucleic acid amplification method Methods 0.000 description 28
- 239000003112 inhibitor Substances 0.000 description 20
- 239000000203 mixture Substances 0.000 description 20
- 230000008569 process Effects 0.000 description 15
- 239000007787 solid Substances 0.000 description 13
- 239000003795 chemical substances by application Substances 0.000 description 12
- 210000000087 hemolymph Anatomy 0.000 description 12
- 108091028043 Nucleic acid sequence Proteins 0.000 description 11
- 230000015572 biosynthetic process Effects 0.000 description 11
- 229940088598 enzyme Drugs 0.000 description 11
- 235000013305 food Nutrition 0.000 description 11
- 238000005382 thermal cycling Methods 0.000 description 11
- 239000003463 adsorbent Substances 0.000 description 10
- 238000004925 denaturation Methods 0.000 description 10
- 230000036425 denaturation Effects 0.000 description 10
- 201000010099 disease Diseases 0.000 description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 9
- 244000005700 microbiome Species 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 108091093088 Amplicon Proteins 0.000 description 8
- 244000144974 aquaculture Species 0.000 description 8
- 238000009360 aquaculture Methods 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 241000439574 Decapod penstyldensovirus 1 Species 0.000 description 7
- 241001465754 Metazoa Species 0.000 description 7
- 102000006382 Ribonucleases Human genes 0.000 description 7
- 108010083644 Ribonucleases Proteins 0.000 description 7
- 238000003556 assay Methods 0.000 description 7
- 239000002299 complementary DNA Substances 0.000 description 7
- 239000012530 fluid Substances 0.000 description 7
- 238000010839 reverse transcription Methods 0.000 description 7
- 230000003381 solubilizing effect Effects 0.000 description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 6
- 241000607618 Vibrio harveyi Species 0.000 description 6
- 230000003187 abdominal effect Effects 0.000 description 6
- 210000002421 cell wall Anatomy 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000000605 extraction Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 208000011580 syndromic disease Diseases 0.000 description 6
- 229920000936 Agarose Polymers 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 244000052616 bacterial pathogen Species 0.000 description 5
- 238000000227 grinding Methods 0.000 description 5
- 239000013641 positive control Substances 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- 244000052613 viral pathogen Species 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 4
- 206010027982 Morphoea Diseases 0.000 description 4
- 108091034117 Oligonucleotide Proteins 0.000 description 4
- 108700026244 Open Reading Frames Proteins 0.000 description 4
- 241000238552 Penaeus monodon Species 0.000 description 4
- 239000013068 control sample Substances 0.000 description 4
- 239000000539 dimer Substances 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 230000004807 localization Effects 0.000 description 4
- 238000002803 maceration Methods 0.000 description 4
- 238000002844 melting Methods 0.000 description 4
- 230000008018 melting Effects 0.000 description 4
- 210000003205 muscle Anatomy 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 150000008300 phosphoramidites Chemical class 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 238000005063 solubilization Methods 0.000 description 4
- 230000007928 solubilization Effects 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- 108091032955 Bacterial small RNA Proteins 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 108020004566 Transfer RNA Proteins 0.000 description 3
- 239000013504 Triton X-100 Substances 0.000 description 3
- 229920004890 Triton X-100 Polymers 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 238000007429 general method Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- 108020004418 ribosomal RNA Proteins 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 238000011896 sensitive detection Methods 0.000 description 3
- 239000001226 triphosphate Substances 0.000 description 3
- 235000011178 triphosphate Nutrition 0.000 description 3
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 239000005909 Kieselgur Substances 0.000 description 2
- 206010028851 Necrosis Diseases 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 238000002944 PCR assay Methods 0.000 description 2
- 239000012807 PCR reagent Substances 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 125000003275 alpha amino acid group Chemical group 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000002820 assay format Methods 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 2
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 2
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 2
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 2
- 239000003398 denaturant Substances 0.000 description 2
- BCAARMUWIRURQS-UHFFFAOYSA-N dicalcium;oxocalcium;silicate Chemical compound [Ca+2].[Ca+2].[Ca]=O.[O-][Si]([O-])([O-])[O-] BCAARMUWIRURQS-UHFFFAOYSA-N 0.000 description 2
- 210000003981 ectoderm Anatomy 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000009830 intercalation Methods 0.000 description 2
- 230000002452 interceptive effect Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 238000007834 ligase chain reaction Methods 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 230000002101 lytic effect Effects 0.000 description 2
- 210000003716 mesoderm Anatomy 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000010079 rubber tapping Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 description 1
- COCMHKNAGZHBDZ-UHFFFAOYSA-N 4-carboxy-3-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate Chemical compound C=12C=CC(=[N+](C)C)C=C2OC2=CC(N(C)C)=CC=C2C=1C1=CC(C([O-])=O)=CC=C1C(O)=O COCMHKNAGZHBDZ-UHFFFAOYSA-N 0.000 description 1
- 229920000298 Cellophane Polymers 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 1
- 201000000628 Gas Gangrene Diseases 0.000 description 1
- 108010078851 HIV Reverse Transcriptase Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 101150092040 ORF94 gene Proteins 0.000 description 1
- 101100263774 Orgyia pseudotsugata multicapsid polyhedrosis virus P18 gene Proteins 0.000 description 1
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102100037968 Ribonuclease inhibitor Human genes 0.000 description 1
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 1
- CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 241000610628 Trichoptilium incisum Species 0.000 description 1
- 108020004417 Untranslated RNA Proteins 0.000 description 1
- 102000039634 Untranslated RNA Human genes 0.000 description 1
- 241000700647 Variola virus Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000009388 chemical precipitation Methods 0.000 description 1
- 231100000481 chemical toxicant Toxicity 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- FFYPMLJYZAEMQB-UHFFFAOYSA-N diethyl pyrocarbonate Chemical compound CCOC(=O)OC(=O)OCC FFYPMLJYZAEMQB-UHFFFAOYSA-N 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 241001493065 dsRNA viruses Species 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- YQOKLYTXVFAUCW-UHFFFAOYSA-N guanidine;isothiocyanic acid Chemical compound N=C=S.NC(N)=N YQOKLYTXVFAUCW-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 206010028320 muscle necrosis Diseases 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000001668 nucleic acid synthesis Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 108010024226 placental ribonuclease inhibitor Proteins 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000009711 regulatory function Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 239000012209 synthetic fiber Substances 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/505—Containers for the purpose of retaining a material to be analysed, e.g. test tubes flexible containers not provided for above
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12823808P | 2008-05-19 | 2008-05-19 | |
| US61/128,238 | 2008-05-19 | ||
| PCT/US2009/044421 WO2009143089A1 (en) | 2008-05-19 | 2009-05-19 | Method, kit and system for collecting and processing samples for dna and rna detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2011520467A true JP2011520467A (ja) | 2011-07-21 |
| JP2011520467A5 JP2011520467A5 (enExample) | 2012-07-12 |
Family
ID=40999774
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011510628A Pending JP2011520467A (ja) | 2008-05-19 | 2009-05-19 | Ｄｎａおよびｒｎａ検出のためにサンプルを収集および処理するための方法、キットならびにシステム |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8895240B2 (enExample) |
| JP (1) | JP2011520467A (enExample) |
| CN (1) | CN102037138A (enExample) |
| BR (1) | BRPI0908618A2 (enExample) |
| WO (1) | WO2009143089A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018139563A (ja) * | 2017-02-28 | 2018-09-13 | 東洋紡株式会社 | Ｒｎアーゼ阻害剤組成物 |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2802550C (en) * | 2010-07-29 | 2019-01-15 | F. Hoffmann-La Roche Ag | Generic pcr |
| KR20140056188A (ko) * | 2011-05-24 | 2014-05-09 | 인제니 피씨알 비.브이. | 물질의 온도를 변화시키는 방법 및 변화시키기 위한 시스템 |
| CN104411406B (zh) | 2012-03-16 | 2017-05-31 | 统计诊断与创新有限公司 | 具有集成传送模块的测试盒 |
| AU2014237362A1 (en) * | 2013-03-15 | 2015-09-17 | Theranos Ip Company, Llc | Devices, systems and methods for sample preparation |
| CA2965657A1 (en) * | 2014-10-28 | 2016-05-06 | Envirologix Inc. | Sample preparation vessels, microfluidic circuits, and systems and methods for sample preparation, extraction, and analysis |
| WO2018056803A1 (en) * | 2016-09-21 | 2018-03-29 | Universiti Malaya | Rapid detection of prawn viruses, method and kit thereof |
| TWI833715B (zh) * | 2017-10-27 | 2024-03-01 | 美商奇諾診療公司 | 用於超低體積液體生物檢體之裝置、系統及方法 |
| CN107988426A (zh) * | 2017-11-10 | 2018-05-04 | 杭州众测生物科技有限公司 | 对虾桃拉病毒（tsv）的raa恒温荧光检测方法及试剂 |
| CN109652405A (zh) * | 2019-01-22 | 2019-04-19 | 南通龙洋生物科技有限公司 | 一种利用南美白对虾血淋巴直接pcr的方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6071698A (en) * | 1996-11-01 | 2000-06-06 | Novartis Finance Corporation | DNA extraction buffer and method of use thereof |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4968615A (en) * | 1985-12-18 | 1990-11-06 | Ciba-Geigy Corporation | Deoxyribonucleic acid segment from a virus |
| US5334499A (en) * | 1989-04-17 | 1994-08-02 | Eastman Kodak Company | Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction |
| US5994056A (en) * | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| FR2694754B1 (fr) * | 1992-08-12 | 1994-09-16 | Bio Merieux | Fragments d'ADN de mycobactéries, amorces d'amplification, sondes d'hybridation, réactifs et procédé de détection de détection de mycobactéries. |
| US6037127A (en) * | 1994-03-31 | 2000-03-14 | E. I. Du Pont De Nemours And Company | Method for detection of non-denatured nucleic acid fragments |
| US5580728A (en) * | 1994-06-17 | 1996-12-03 | Perlin; Mark W. | Method and system for genotyping |
| US5824535A (en) * | 1996-01-17 | 1998-10-20 | Kou; Guang-Hsiung | Identification, purification and detection of WSBV (Baculovirus associated with white spot syndrome) |
| CA2225189C (en) * | 1997-03-06 | 2010-05-25 | Queen's University At Kingston | Canine factor viii gene, protein and methods of use |
| US6894156B2 (en) * | 1999-04-15 | 2005-05-17 | E. I. Du Pont De Nemours And Company | Nucleic acid fragments for the identification of dechlorinating bacteria |
| US6793387B1 (en) * | 1999-05-08 | 2004-09-21 | Chata Biosystems, Inc. | Apparatus for automatic preparation of a mixture and method |
| US20020132769A1 (en) * | 2000-10-06 | 2002-09-19 | Michael Kaleko | Targeting molecules |
| US20040010815A1 (en) * | 2001-09-26 | 2004-01-15 | Lange B. Markus | Identification and characterization of plant genes |
| US20040194156A1 (en) * | 2002-12-27 | 2004-09-30 | Califa Llc | Transgenic aquatic invertebrates as a bioreactor for production of recombinant polypeptides |
| US20040166520A1 (en) * | 2003-01-03 | 2004-08-26 | Connolly D. Michael | Identifying items with nucleic acid taggants |
| CN1179052C (zh) * | 2003-03-06 | 2004-12-08 | 中国科学院武汉病毒研究所 | 罗氏沼虾诺达病毒核酸rt-pcr检测的方法 |
| US7575864B2 (en) * | 2004-05-27 | 2009-08-18 | E.I. Du Pont De Nemours And Company | Method for the direct detection of diagnostic RNA |
| JP2010501178A (ja) | 2006-08-24 | 2010-01-21 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | エビ病原体の診断用配列 |
| US8057991B2 (en) | 2006-08-24 | 2011-11-15 | E. I. Du Pont De Nemours And Company | Sequences diagnostic for shrimp pathogens |
| CN101611155B (zh) | 2006-08-24 | 2013-05-15 | 纳幕尔杜邦公司 | 虾病原体诊断用序列 |
| US7709203B2 (en) * | 2006-10-20 | 2010-05-04 | E.I. Du Pont De Nemours And Company | Sequences diagnostic for shrimp pathogens |
-
2009
- 2009-05-18 US US12/467,711 patent/US8895240B2/en not_active Expired - Fee Related
- 2009-05-19 JP JP2011510628A patent/JP2011520467A/ja active Pending
- 2009-05-19 CN CN200980117982XA patent/CN102037138A/zh active Pending
- 2009-05-19 BR BRPI0908618A patent/BRPI0908618A2/pt not_active Application Discontinuation
- 2009-05-19 WO PCT/US2009/044421 patent/WO2009143089A1/en not_active Ceased
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6071698A (en) * | 1996-11-01 | 2000-06-06 | Novartis Finance Corporation | DNA extraction buffer and method of use thereof |
Non-Patent Citations (1)
| Title |
|---|
| JPN6014003580; Ｄｉｓｅａｓｅｓ ｏｆ ａｑｕａｔｉｃ ｏｒｇａｎｉｓｍｓ． ２００６， Ｖｏｌ．７１， Ｎｏ．２， ｐ．９１-１００ * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018139563A (ja) * | 2017-02-28 | 2018-09-13 | 東洋紡株式会社 | Ｒｎアーゼ阻害剤組成物 |
| JP2021100965A (ja) * | 2017-02-28 | 2021-07-08 | 東洋紡株式会社 | Ｒｎアーゼ阻害剤組成物 |
| JP7036259B2 (ja) | 2017-02-28 | 2022-03-15 | 東洋紡株式会社 | Ｒｎアーゼ阻害剤組成物 |
Also Published As
| Publication number | Publication date |
|---|---|
| US8895240B2 (en) | 2014-11-25 |
| US20100028887A1 (en) | 2010-02-04 |
| WO2009143089A1 (en) | 2009-11-26 |
| BRPI0908618A2 (pt) | 2015-09-15 |
| CN102037138A (zh) | 2011-04-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8895240B2 (en) | Method, kit and system for collecting and processing samples for DNA and RNA detection | |
| TWI697560B (zh) | 用於偵測細菌污染之方法及組合物 | |
| JP5133991B2 (ja) | エビ病原体の診断用配列 | |
| US8309301B2 (en) | Sequences diagnostic for shrimp pathogens | |
| WO2004072230A2 (en) | Real-time polymerase chain reaction using large target amplicons | |
| US7951540B2 (en) | Sequences diagnostic for shrimp pathogens | |
| JP2020156470A (ja) | 非特異増幅が抑制された核酸増幅法 | |
| JP2011520467A5 (enExample) | ||
| JP2024026545A (ja) | 改良された核酸検出方法 | |
| CN114080456A (zh) | Rna病毒检测方法 | |
| CN101506387B (zh) | 用于诊断虾病原体的序列 | |
| Pusch et al. | Quantitative detection of enteroviruses in activated sludge by cell culture and real-time RT-PCR using paramagnetic capturing | |
| CN101619349A (zh) | 一种荧光实时定量pcr检测香港海鸥型菌的试剂盒及检测方法 | |
| JP4903722B2 (ja) | ウイルスを用いることによる試料中の生細胞を検出するための方法 | |
| CN111254217A (zh) | 诺如病毒的检测方法 | |
| CN105838803A (zh) | 一种致病哈氏弧菌特异性分子遗传标记及其应用 | |
| Umesha et al. | Detection of Leptospira in Canine Samples using Polymerase Chain Reaction based on LipL32gene and Biochemical Profiles in Canine Leptospirosis | |
| JP2005073595A (ja) | 核酸試料調製法及び核酸増幅法 | |
| EP4267756A1 (en) | Detecting a target nucleic acid in a biological sample | |
| HK1136318B (en) | Sequences diagnostic for shrimp pathogens | |
| HK1137781A (en) | Sequences diagnostic for shrimp pathogens | |
| HK1137781B (en) | Sequences diagnostic for shrimp pathogens | |
| HK1137488B (en) | Sequences diagnostic for shrimp pathogens |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20120521 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20120521 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140204 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140425 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20141007 |