JP2011510626A - Snpアレイを用いた染色体、遺伝子または特定ヌクレオチド配列のコピー数測定方法 - Google Patents
Snpアレイを用いた染色体、遺伝子または特定ヌクレオチド配列のコピー数測定方法 Download PDFInfo
- Publication number
- JP2011510626A JP2011510626A JP2010544244A JP2010544244A JP2011510626A JP 2011510626 A JP2011510626 A JP 2011510626A JP 2010544244 A JP2010544244 A JP 2010544244A JP 2010544244 A JP2010544244 A JP 2010544244A JP 2011510626 A JP2011510626 A JP 2011510626A
- Authority
- JP
- Japan
- Prior art keywords
- dna
- chromosome
- copy number
- snp
- homozygous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2545/00—Reactions characterised by their quantitative nature
- C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
- C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Plant Pathology (AREA)
Abstract
【解決手段】
本発明は、以下の工程:(a)ホモ接合DNAを検体試料DNAと混合する工程;(b)該DNA混合物をSNPアレイで分析する工程;そして(c)ホモ接合DNAからのシグナルと検体試料DNAからのシグナルとの差異を測定して染色体、遺伝子または特定ヌクレオチド配列のコピー数を決定する工程を含む、染色体、遺伝子または特定ヌクレオチド配列のコピー数測定方法に関する。
【選択図】図3
Description
本発明の目的は、以下のステップ:(a)ホモ接合DNAを検体試料DNAと混合する工程;(b)該DNA混合物をSNPアレイで分析する工程;そして(c)ホモ接合DNAからのシグナルと検体試料DNAからのシグナルとの差異を測定して染色体、遺伝子または特定ヌクレオチド配列のコピー数を決定する工程とを含んでなる、染色体、遺伝子または特定ヌクレオチド配列のコピー数測定方法を提供することである。
一つの見地では、上記目的を達成するために、本発明は、以下のステップ:(a)ホモ接合DNAを検体試料DNAと混合する工程;(b)該DNA混合物をSNPアレイで分析する工程;そして(c)ホモ接合DNAからのシグナルと検体試料DNAからのシグナルとの差異を測定して、染色体、遺伝子または特定ヌクレオチド配列のコピー数を決定する工程とを含む、染色体、遺伝子または特定ヌクレオチド配列のコピー数測定方法に関する。
正常の検体試料DNAを単独で分析し、また正常の検体試料DNAとホモ接合DNAとの1:1混合物を分析した後、各アレルの相対的なシグナル強度を示した。正常な検体試料(normal)におけるアレルのシグナル強度は、図1の左側に示した。コピー数がトリソミーに変化した場合のアレルの相対的なシグナル強度を、図の右側に示した。ここで各DNA鎖の変化を図式化した。検体試料DNAとホモ接合DNAの全てのアレルに対する情報を分析した後、さらに本発明の方法に従って、各アレルをSNPアレイで分析した。コピー数の測定が可能な場合をoで表した。コピー数の測定が不可能な場合をxで表示した。pは、特定のアレルの相対的シグナル強度からはコピー数の増加または減少を決定することはできないが、変化したということは分かる場合であって、全シグナルの強度によって増加または減少を決定できる場合である。
各アレルの相対的シグナル強度として一つのアレルの欠失が認められ、この場合の各DNA鎖における欠失を分けて図式化した。検体試料のみをもってSNPアレイを実施したとき、一つのアレルの結果からは欠失したことを決定することができず、隣接したアレルの分析によって欠失の可能性を決定することができた。ところが、本発明の方法を用いた場合、各アレルを分析することができ、それによって欠失を決定することができ、さらに以前の方法に比べて分解能が激的に改善されることが分かる(図2)。
ホモ接合細胞株としての胞状奇胎細胞株のDNAと、21番染色体の3コピーを持つダウン症候群患者のDNAのそれぞれとを1:1で混合した後、SNPアレイを用いてコピー数を分析した。21番染色体とX染色体を除いた場合、SRが1:1、1:3、3:1であることを確認した。21番染色体はSRが2:3、3:2、1:4、4:1であることを確認した。ここでSRが2:3または3:2であることは、ダウン症候群患者における21番染色体が3コピーあることを示している(図4)。
ダウン症候群DNAと正常のコントロールDNAを試料として用い、これを胞状奇胎DNAとそれぞれ混合した(1:1)。次いでIllumina SNP array(317K Duo)を用いてSNP分析を実施した。胞状奇胎ではAAを示すものを、ダウン症候群ではBBを示すもののみを別途分析した。1番染色体および21番染色体から300個の対応するSNPsをそれぞれ抽出して分析した(図5)。その結果、21番染色体は、ダウン症候群において追加されており(3n)、正常な染色体(2n)である1番染色体とは、コピー数の状態において容易に区別された。
Claims (7)
- 以下の工程:
(a)ホモ接合DNAを検体試料DNAと混合する工程;
(b)該DNA混合物をSNPアレイで分析する工程;そして
(c)ホモ接合DNAからのシグナルと検体試料DNAからのシグナルとの差異を測定して、染色体、遺伝子または特定ヌクレオチド配列のコピー数を決定する工程
を含む、染色体、遺伝子または特定ヌクレオチド配列のコピー数測定方法。 - ホモ接合DNAが単為生殖細胞株または胞状奇胎細胞株のDNAであることを特徴とする、請求項1に記載の方法。
- ホモ接合DNAがクローニングされたDNA断片であることを特徴とする、請求項1に記載の方法。
- (a)工程において、ホモ接合DNAを検体試料DNAと1:1の比率で混合することを特徴とする、請求項1に記載の方法。
- SNPアレイがレアSNPを測定するように考案されたSNPアレイであることを特徴とする、請求項1に記載の方法。
- (c)工程において、ホモ接合DNAおよび検体試料DNAが異型のホモ接合アレルである場合、
ホモ接合DNAおよび検体試料DNAのSNPアレル間のシグナル比(SR)は、以下の式
- 検体試料DNAがヘテロ接合アレルの場合、2つの塩基からのシグナル強度を他の領域で測定した値と比較して、染色体、遺伝子または特定ヌクレオチド配列のコピー数における変化を決定することを特徴とする、請求項1に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR20080022500 | 2008-03-11 | ||
KR10-2008-0022500 | 2008-03-11 | ||
PCT/KR2009/001111 WO2009113779A2 (ko) | 2008-03-11 | 2009-03-05 | Snp 어레이를 이용한 염색체, 유전자, 또는 특정 뉴클레오티드 서열의 카피 수 측정방법 |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2011510626A true JP2011510626A (ja) | 2011-04-07 |
JP5683964B2 JP5683964B2 (ja) | 2015-03-11 |
Family
ID=41065650
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2010544244A Expired - Fee Related JP5683964B2 (ja) | 2008-03-11 | 2009-03-05 | Snpアレイを用いた染色体、遺伝子または特定ヌクレオチド配列のコピー数測定方法 |
Country Status (8)
Country | Link |
---|---|
US (1) | US9012370B2 (ja) |
EP (1) | EP2253713B1 (ja) |
JP (1) | JP5683964B2 (ja) |
KR (1) | KR101030476B1 (ja) |
CN (1) | CN101918597B (ja) |
AU (1) | AU2009224170B2 (ja) |
CA (1) | CA2710807C (ja) |
WO (1) | WO2009113779A2 (ja) |
Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5888769B2 (ja) * | 2011-09-20 | 2016-03-22 | 国立大学法人京都大学 | 相同組換えにより遺伝子改変された多能性幹細胞の簡便な検出法 |
US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
WO2014124336A2 (en) | 2013-02-08 | 2014-08-14 | 10X Technologies, Inc. | Partitioning and processing of analytes and other species |
US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
CN110548550B (zh) | 2014-04-10 | 2022-03-08 | 10X基因组学有限公司 | 用于封装和分割试剂的流体装置、系统和方法及其应用 |
KR101663171B1 (ko) * | 2014-05-27 | 2016-10-14 | 이원 다이애그노믹스 게놈센타(주) | 다운증후군 진단을 위한 바이오마커 및 그의 용도 |
CA2953374A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
MX2016016713A (es) | 2014-06-26 | 2017-05-23 | 10X Genomics Inc | Procesos y sistemas para el montaje de secuencias de acido nucleico. |
MX367432B (es) | 2015-01-12 | 2019-08-08 | 10X Genomics Inc | Procesos y sistemas para la preparación de bibliotecas de secuenciación de ácido nucleico y bibliotecas preparadas con estos. |
EP3245605B1 (en) | 2015-01-13 | 2022-04-20 | 10X Genomics, Inc. | Systems and methods for visualizing structural variation and phasing information |
EP3256606B1 (en) | 2015-02-09 | 2019-05-22 | 10X Genomics, Inc. | Systems and methods for determining structural variation |
CN108779491B (zh) | 2016-02-11 | 2021-03-09 | 10X基因组学有限公司 | 用于全基因组序列数据的从头组装的系统、方法和介质 |
US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
EP3625715A4 (en) | 2017-05-19 | 2021-03-17 | 10X Genomics, Inc. | DATA SET ANALYSIS SYSTEMS AND METHODS |
EP3625361A1 (en) | 2017-11-15 | 2020-03-25 | 10X Genomics, Inc. | Functionalized gel beads |
US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
WO2019108851A1 (en) | 2017-11-30 | 2019-06-06 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation and analysis |
US11639928B2 (en) | 2018-02-22 | 2023-05-02 | 10X Genomics, Inc. | Methods and systems for characterizing analytes from individual cells or cell populations |
US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
US11655499B1 (en) | 2019-02-25 | 2023-05-23 | 10X Genomics, Inc. | Detection of sequence elements in nucleic acid molecules |
AU2022227563A1 (en) | 2021-02-23 | 2023-08-24 | 10X Genomics, Inc. | Probe-based analysis of nucleic acids and proteins |
KR20240016220A (ko) * | 2022-07-28 | 2024-02-06 | 국립암센터 | 차세대 염기서열분석 패널의 검증 방법 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006011738A1 (en) * | 2004-07-26 | 2006-02-02 | Kyeong Man Hong | Method for measuring the chromosome, gene or nucleotide sequence copy numbers using co- amplification of artificial snp sequences |
Family Cites Families (66)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US1359801A (en) | 1920-03-03 | 1920-11-23 | Goldner Joe | Combination mattress and pillow |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US5333675C1 (en) | 1986-02-25 | 2001-05-01 | Perkin Elmer Corp | Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps |
US5721098A (en) | 1986-01-16 | 1998-02-24 | The Regents Of The University Of California | Comparative genomic hybridization |
US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
US5856092A (en) | 1989-02-13 | 1999-01-05 | Geneco Pty Ltd | Detection of a nucleic acid sequence or a change therein |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5242974A (en) | 1991-11-22 | 1993-09-07 | Affymax Technologies N.V. | Polymer reversal on solid surfaces |
US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
US5527681A (en) | 1989-06-07 | 1996-06-18 | Affymax Technologies N.V. | Immobilized molecular synthesis of systematically substituted compounds |
US6040138A (en) | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
US6309822B1 (en) | 1989-06-07 | 2001-10-30 | Affymetrix, Inc. | Method for comparing copy number of nucleic acid sequences |
US6346413B1 (en) | 1989-06-07 | 2002-02-12 | Affymetrix, Inc. | Polymer arrays |
US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
US5424186A (en) | 1989-06-07 | 1995-06-13 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis |
US5252743A (en) | 1989-11-13 | 1993-10-12 | Affymax Technologies N.V. | Spatially-addressable immobilization of anti-ligands on surfaces |
US5817462A (en) | 1995-02-21 | 1998-10-06 | Applied Spectral Imaging | Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding |
US5412087A (en) | 1992-04-24 | 1995-05-02 | Affymax Technologies N.V. | Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces |
US5384261A (en) | 1991-11-22 | 1995-01-24 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis using mechanically directed flow paths |
US5550215A (en) | 1991-11-22 | 1996-08-27 | Holmes; Christopher P. | Polymer reversal on solid surfaces |
US5324633A (en) | 1991-11-22 | 1994-06-28 | Affymax Technologies N.V. | Method and apparatus for measuring binding affinity |
ATE262374T1 (de) | 1991-11-22 | 2004-04-15 | Affymetrix Inc | Kombinatorische strategien für polymersynthese |
US5491074A (en) | 1993-04-01 | 1996-02-13 | Affymax Technologies Nv | Association peptides |
US5837832A (en) | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
WO1995000530A1 (en) | 1993-06-25 | 1995-01-05 | Affymax Technologies N.V. | Hybridization and sequencing of nucleic acids |
US5858659A (en) | 1995-11-29 | 1999-01-12 | Affymetrix, Inc. | Polymorphism detection |
DE4344726C2 (de) | 1993-12-27 | 1997-09-25 | Deutsches Krebsforsch | Verfahren zum Nachweis von nicht balanciertem genetischen Material einer Spezies oder zum Nachweis der Genexpression in Zellen einer Spezies |
US5578832A (en) | 1994-09-02 | 1996-11-26 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
US6090555A (en) | 1997-12-11 | 2000-07-18 | Affymetrix, Inc. | Scanned image alignment systems and methods |
US5631734A (en) | 1994-02-10 | 1997-05-20 | Affymetrix, Inc. | Method and apparatus for detection of fluorescently labeled materials |
WO1995030774A1 (en) | 1994-05-05 | 1995-11-16 | Beckman Instruments, Inc. | Oligonucleotide repeat arrays |
US5571639A (en) | 1994-05-24 | 1996-11-05 | Affymax Technologies N.V. | Computer-aided engineering system for design of sequence arrays and lithographic masks |
US5795716A (en) | 1994-10-21 | 1998-08-18 | Chee; Mark S. | Computer-aided visualization and analysis system for sequence evaluation |
WO1996015271A1 (en) | 1994-11-16 | 1996-05-23 | Abbott Laboratories | Multiplex ligations-dependent amplification |
US5599695A (en) | 1995-02-27 | 1997-02-04 | Affymetrix, Inc. | Printing molecular library arrays using deprotection agents solely in the vapor phase |
US5959098A (en) | 1996-04-17 | 1999-09-28 | Affymetrix, Inc. | Substrate preparation process |
US5624711A (en) | 1995-04-27 | 1997-04-29 | Affymax Technologies, N.V. | Derivatization of solid supports and methods for oligomer synthesis |
US5968740A (en) | 1995-07-24 | 1999-10-19 | Affymetrix, Inc. | Method of Identifying a Base in a Nucleic Acid |
US5733729A (en) | 1995-09-14 | 1998-03-31 | Affymetrix, Inc. | Computer-aided probability base calling for arrays of nucleic acid probes on chips |
US6300063B1 (en) | 1995-11-29 | 2001-10-09 | Affymetrix, Inc. | Polymorphism detection |
US6147205A (en) | 1995-12-15 | 2000-11-14 | Affymetrix, Inc. | Photocleavable protecting groups and methods for their use |
EP0902885A4 (en) | 1996-05-16 | 2006-09-27 | Affymetrix Inc | SYSTEMS AND METHODS FOR DETECTION OF BRANDED PRODUCTS |
WO1998056954A1 (en) | 1997-06-13 | 1998-12-17 | Affymetrix, Inc. | Method to detect gene polymorphisms and monitor allelic expression employing a probe array |
US6333179B1 (en) | 1997-06-20 | 2001-12-25 | Affymetrix, Inc. | Methods and compositions for multiplex amplification of nucleic acids |
DE69823206T2 (de) | 1997-07-25 | 2004-08-19 | Affymetrix, Inc. (a Delaware Corp.), Santa Clara | Verfahren zur herstellung einer bio-informatik-datenbank |
US6223127B1 (en) | 1997-08-15 | 2001-04-24 | Affymetrix, Inc. | Polymorphism detection utilizing clustering analysis |
DE69829402T2 (de) | 1997-10-31 | 2006-04-13 | Affymetrix, Inc. (a Delaware Corp.), Santa Clara | Expressionsprofile in adulten und fötalen organen |
US6013449A (en) | 1997-11-26 | 2000-01-11 | The United States Of America As Represented By The Department Of Health And Human Services | Probe-based analysis of heterozygous mutations using two-color labelling |
US6428752B1 (en) | 1998-05-14 | 2002-08-06 | Affymetrix, Inc. | Cleaning deposit devices that form microarrays and the like |
US6269846B1 (en) | 1998-01-13 | 2001-08-07 | Genetic Microsystems, Inc. | Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays |
US6201639B1 (en) | 1998-03-20 | 2001-03-13 | James W. Overbeck | Wide field of view and high speed scanning microscopy |
US6185030B1 (en) | 1998-03-20 | 2001-02-06 | James W. Overbeck | Wide field of view and high speed scanning microscopy |
US6020135A (en) | 1998-03-27 | 2000-02-01 | Affymetrix, Inc. | P53-regulated genes |
US5936324A (en) | 1998-03-30 | 1999-08-10 | Genetic Microsystems Inc. | Moving magnet scanner |
US6185561B1 (en) | 1998-09-17 | 2001-02-06 | Affymetrix, Inc. | Method and apparatus for providing and expression data mining database |
US6262216B1 (en) | 1998-10-13 | 2001-07-17 | Affymetrix, Inc. | Functionalized silicon compounds and methods for their synthesis and use |
WO2000024939A1 (en) | 1998-10-27 | 2000-05-04 | Affymetrix, Inc. | Complexity management and analysis of genomic dna |
US6177248B1 (en) | 1999-02-24 | 2001-01-23 | Affymetrix, Inc. | Downstream genes of tumor suppressor WT1 |
WO2000058516A2 (en) | 1999-03-26 | 2000-10-05 | Whitehead Institute For Biomedical Research | Universal arrays |
US6218803B1 (en) | 1999-06-04 | 2001-04-17 | Genetic Microsystems, Inc. | Position sensing with variable capacitance transducers |
US20030143554A1 (en) | 2001-03-31 | 2003-07-31 | Berres Mark E. | Method of genotyping by determination of allele copy number |
US7822555B2 (en) | 2002-11-11 | 2010-10-26 | Affymetrix, Inc. | Methods for identifying DNA copy number changes |
DE102005051816A1 (de) | 2005-10-28 | 2007-05-10 | Advalytix Ag | Verfahren zur relativen Bestimmung der Kopienzahl einer vorbestimmten Sequenz in einer biologischen Probe |
DE102005059227A1 (de) | 2005-12-12 | 2007-06-14 | Advalytix Ag | Verfahren zur Bestimmung des Genotyps aus einer biologischen Probe enthaltend Nukleinsäuren unterschiedlicher Individuen |
-
2009
- 2009-03-05 JP JP2010544244A patent/JP5683964B2/ja not_active Expired - Fee Related
- 2009-03-05 WO PCT/KR2009/001111 patent/WO2009113779A2/ko active Application Filing
- 2009-03-05 EP EP09718746.2A patent/EP2253713B1/en not_active Not-in-force
- 2009-03-05 KR KR1020090018949A patent/KR101030476B1/ko not_active IP Right Cessation
- 2009-03-05 CA CA2710807A patent/CA2710807C/en not_active Expired - Fee Related
- 2009-03-05 US US12/810,229 patent/US9012370B2/en not_active Expired - Fee Related
- 2009-03-05 AU AU2009224170A patent/AU2009224170B2/en not_active Ceased
- 2009-03-05 CN CN200980101478.0A patent/CN101918597B/zh not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006011738A1 (en) * | 2004-07-26 | 2006-02-02 | Kyeong Man Hong | Method for measuring the chromosome, gene or nucleotide sequence copy numbers using co- amplification of artificial snp sequences |
Non-Patent Citations (4)
Title |
---|
JPN6013004009; J. Translat. Med. Vol.1, No.4, 2003, p.1-14 * |
JPN6013004010; Gen. Res. Vol.9, 1999, p.499-505 * |
JPN6013004011; Can. Res. Vol.64, 2004, p.3060-3071 * |
JPN6013004012; Nat. Vol.444, 200611, p.444-454 * |
Also Published As
Publication number | Publication date |
---|---|
US20110105352A1 (en) | 2011-05-05 |
KR20090097792A (ko) | 2009-09-16 |
CA2710807C (en) | 2015-09-08 |
EP2253713B1 (en) | 2015-02-25 |
CN101918597B (zh) | 2013-09-18 |
AU2009224170B2 (en) | 2012-03-29 |
WO2009113779A2 (ko) | 2009-09-17 |
JP5683964B2 (ja) | 2015-03-11 |
KR101030476B1 (ko) | 2011-04-25 |
AU2009224170A1 (en) | 2009-09-17 |
US9012370B2 (en) | 2015-04-21 |
CN101918597A (zh) | 2010-12-15 |
WO2009113779A3 (ko) | 2009-12-10 |
EP2253713A2 (en) | 2010-11-24 |
EP2253713A4 (en) | 2012-03-21 |
CA2710807A1 (en) | 2009-09-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5683964B2 (ja) | Snpアレイを用いた染色体、遺伝子または特定ヌクレオチド配列のコピー数測定方法 | |
RU2733887C2 (ru) | Мультиплексное детектирование нуклеиновых кислот | |
JP5262230B2 (ja) | 新規多型検出法 | |
JP5759500B2 (ja) | 緑内障進行リスクの判定方法 | |
JP2009519710A (ja) | 遺伝子発現調節エレメントのハイスループットでの特徴付けのための機能性アレイ | |
US20040229224A1 (en) | Allele-specific expression patterns | |
RU2577726C2 (ru) | Генетические полиморфизмы при возрастной дегенерации желтого пятна | |
Brion et al. | New technologies in the genetic approach to sudden cardiac death in the young | |
JP2009516516A (ja) | ヌクレオチド配列を同定するための方法及びプローブ | |
Ness | Microarray analysis: basic strategies for successful experiments | |
EP1829979B1 (en) | Method of identifying gene with variable expression | |
EP2059608A1 (en) | Use of snps for the diagnosis of a pain protective haplotype in the gtp cyclohydrolase 1 gene (gch1) | |
Yang et al. | Copy number variation | |
EP2397561B1 (en) | Methods for determining the presence or absence of genetic segments | |
Soteh et al. | Mutational analysis of the von Willebrand factor gene in type 1 von Willebrand disease using conformation sensitive gel electrophoresis: a comparison of fluorescent and manual techniques | |
US8221978B2 (en) | Normalization probes for comparative genome hybridization arrays | |
JP4972737B2 (ja) | Th2サイトカイン阻害剤への感受性の検査方法 | |
WO2017120750A1 (zh) | 一种针对东亚人群全基因组范围内的非编码区的SNPs的DNA芯片 | |
US8216787B2 (en) | Biomarker for successful aging without cognitive decline | |
Horvath et al. | Basic molecular techniques for the detection of single nucleotide polymorphisms: genome-wide applications in search for endocrine tumor related genes | |
Uziela | Making microarray and RNA-seq gene expression data comparable | |
Mariana et al. | DESENVOLVIMENTO DE UM MÉTODO DE DETEÇÃO DE CNVs ATRAVÉS DE qPCR |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20130205 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20130507 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20140128 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20140428 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20140508 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20140528 |
|
A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20140604 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140627 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20150106 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20150114 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 5683964 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
LAPS | Cancellation because of no payment of annual fees |