JP2011157299A - Humectant, anti-aging agent, antioxidant, skin whitening agent, antiinflammatory agent, skin external preparation and functional oral composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a humectant, an anti-aging agent, an antioxidant, a skin whitening agent, and an antiinflammatory agent which can be widely applied to the fields of skin external preparations, functional oral compositions and the like. <P>SOLUTION: The humectant, the anti-aging agent, the antioxidant, the skin whitening agent, the antiinflammatory agent, the skin external preparation and the functional oral composition include an extract of a bulb of a plant belonging to the genus Freesia in the family Iridaceae. <P>COPYRIGHT: (C)2011,JPO&INPIT

Description

  The present invention relates to a moisturizer, an anti-aging agent, an antioxidant, a whitening agent, an anti-inflammatory agent, an external preparation for skin, and a functional oral composition containing bulbs of Iridaceae freesia plants (genus Freesia) or extracts thereof. .

  Conventionally, in order to provide a moisturizing ingredient that prevents the skin from drying and moisturizes the skin, various active ingredients have been studied. Also, as factors of skin symptoms such as aging, disease, stress, wrinkles due to ultraviolet rays, blemishes, reduced skin elasticity, dryness, cellular function decline, melanin production and pigmentation due to ultraviolet rays, decrease and degeneration of dermal matrix components, Examples include cell oxidative damage caused by ultraviolet rays. In order to prevent and ameliorate such skin symptoms, various active ingredients have been searched and formulated. In particular, naturally-derived components are known to have various pharmacological and cosmetic effects, and the application of extracts such as plants and fungi to skin external preparations and oral compositions has been studied so far.

  For example, cell water (refer to Patent Document 1), which is a distilled fraction obtained by distilling citrus husks under reduced pressure as a moisturizing agent with excellent skin moisturizing effect and safety, prevents skin aging and improves it. In order to obtain an external preparation for skin, a liquid (see Patent Document 2) obtained by separating from a cucurbitaceae fruit juice juice as an ingredient having an activity of dermal fibroblast activation or growth promotion, or epidermis keratinocytes As a component having an activation or growth promoting action, a peanut extract (see Patent Document 3) and the like are disclosed. Examples of whitening agents include wisteria tea extract (see Patent Document 4) as an ingredient having an inhibitory action on tyrosinase activity, germinated broccoli-derived ingredients (see Patent Document 5) as an ingredient having an inhibitory action on melanin production, and hyaluronidase as an anti-inflammatory agent. Shiratamakazura (see Patent Document 6) and the like are already known as components having an inhibitory action. Antioxidants include, for example, the essence of Iridaceae plants as a component having an action of eliminating hydrogen oxide (see Patent Document 7), or a freesia flower singulated as a component having a radical scavenging action (see Patent Document 8). Has been known so far, but the ingredients obtained from freesia bulbs have not been known so far.

JP 2009-196898 A JP 2004-51492 A JP 2006-316028 A JP 2002-370962 A JP 2003-155221 A JP 2006-36713 A Japanese Patent Laid-Open No. 13-131046 Japanese Patent Laid-Open No. 10-072335

  Thus, various naturally-derived components have been applied so far. However, there are many naturally-derived ingredients whose effects are not yet known, and the development of active ingredients with excellent moisturizing, anti-aging, antioxidant, whitening and anti-inflammatory effects It was expected.

  As a result of examining various components derived from nature, the present inventors have found that moisturizing action, anti-aging action, which is excellent in bulbs of the genus Iridaceae freesia, or its extract, whose effects were not known in the past, The present inventors have found that an antioxidant action, a whitening action and an anti-inflammatory action exist, and have further studied to complete the present invention.

  That is, the present invention provides a moisturizer, an anti-aging agent, an antioxidant, a whitening agent, an anti-inflammatory agent, a skin external preparation, and at least one plant bulb selected from the Iridaceae freesia plant or an extract thereof. It relates to a functional oral composition.

  According to the present invention, a moisturizer, an anti-aging agent, an anti-oxidant having an excellent effect by blending bulbs of one or more kinds of plants selected from Iridaceae freesia plants or extracts thereof, A whitening agent, an anti-inflammatory agent, an external preparation for skin, and a functional oral composition can be provided.

  The Freesia plant used in the present invention is a monocot bulbous plant belonging to the Iridaceae family and is a perennial bulbous plant. Many cultivars are known in addition to the original species Freesia (Freesia refracta) and Freesia armstrengii (Freesia armstrongii).

  Although this invention will not be specifically limited if it is a Freesia genus plant, From the point of the effect of this invention, the 1 type, or 2 or more types of plant selected from Freesia and its cultivar is mentioned as a suitable thing.

  When these freesia plants are used, bulbs are used.

  In extraction, plant bulbs may be used as they are, but considering extraction efficiency, it is preferable to perform extraction after processing such as shredding, drying, and pulverization.

  The extraction can be performed by immersing in an arbitrary extraction solvent for a predetermined time. The extraction solvent may be heated as necessary. Alternatively, extraction can be performed using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be stirred or homogenized in an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is suitably about 1 hour to 14 days.

  As an extraction solvent, in addition to water, lower alcohols such as methanol, ethanol, propanol and isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin; ethers such as ethyl ether and propyl ether Solvents such as esters; esters such as butyl acetate and ethyl acetate; ketones such as acetone and ethyl methyl ketone can be used. These may be used alone or in combination of any two or more. Saline, phosphate buffer, phosphate buffered saline, and the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical liquids and subcritical liquids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  Extracts of freesia plant bulbs with the above solvents can be used as they are, but they can be left to stand for a certain period of time and aged, or concentrated and dried to water or a polar solvent. It can be dissolved again and used. Alternatively, it may be used after performing purification treatment such as decolorization, deodorization, and desalting, and fractionation treatment by column chromatography or the like within a range not impairing these physiological functions. The above-mentioned extract of bulbs of the genus Freesia and the processed products and fractions thereof can be freeze-dried after each treatment and fractionation and dissolved in a solvent at the time of use. It can also be used by encapsulating in vesicles such as liposomes or microcapsules.

  Freesia plant bulb or its extract has excellent moisturizing, anti-aging, antioxidant, whitening, anti-inflammatory, moisturizing, anti-aging, antioxidant, whitening, anti-inflammatory It can be used as an agent, an external preparation for skin and a functional oral composition.

  A moisturizing agent containing a bulb of a freesia genus plant or an extract thereof as an active ingredient has an excellent human dermal fibroblast hyaluronic acid production promoting action and exhibits an excellent moisturizing effect.

  An anti-aging agent comprising a bulb of a freesia plant or an extract thereof as an active ingredient is an excellent cell activating action of human dermal fibroblasts, an action of promoting human dermal fibroblast type I collagen production, and an action of activating human epidermal keratinocytes It has an excellent effect in preventing and improving aging symptoms.

  An antioxidant comprising a freesia plant bulb or an extract thereof as an active ingredient has an excellent DPPH radical scavenging action and an SOD-like activity action, and exhibits an excellent antioxidant effect.

  A whitening agent comprising a bulb of a freesia plant or an extract thereof as an active ingredient has an excellent human epidermal melanocyte tyrosinase activity inhibitory action and a B16 mouse melanoma cell melanin production inhibitory action, and exhibits an excellent whitening effect.

  An anti-inflammatory agent comprising a bulb of a Freesia genus plant or an extract thereof as an active ingredient has an excellent hyaluronidase inhibitory action and exhibits an excellent anti-inflammatory effect.

  A skin external preparation containing a bulb of a freesia plant or an extract thereof exhibits excellent moisturizing action, anti-aging action, antioxidant action, whitening action, anti-inflammatory action and the like.

  A functional oral composition containing a bulb of a freesia plant or an extract thereof exhibits excellent moisturizing action, anti-aging action, antioxidant action, whitening action, anti-inflammatory action and the like.

  As long as these agents contain bulbs of Freesia plants or extracts thereof as active ingredients, the form and presence / absence of other ingredients are not limited. As for the form, any form such as liquid, paste, gel, solid, powder, etc. can be selected according to its use and the like, vehicle (excipient), solvent, Other general additives (antioxidants, colorants, dispersants, etc.) can optionally be included.

  Here, the external preparation for skin means all external compositions for external use on the skin or hair, such as cosmetics, quasi-drugs, and external medicines. The functional oral composition also means all compositions that are taken orally regardless of the type of pharmaceuticals, foods, beverages and the like.

  The dosage form of the external preparation for skin is arbitrary, and can be provided, for example, as a solubilizing system such as lotion, a dispersion system such as calamine lotion, or an emulsifying system such as cream or emulsion. Further, it can be provided in various dosage forms such as an aerosol form, an ointment, and a poultice filled with a propellant.

  Specifically, various cosmetics such as emulsions, creams, lotions, lotions, packs, cosmetic liquids, cleaning agents, makeup cosmetics, etc .; liquids, ointments, powders, granules, aerosols, patches, poultices, etc. Various forms of cosmetics, quasi drugs, topical medicines, and the like can be exemplified.

  The form of the functional oral composition is also arbitrary and is not particularly limited. Specifically, general foods including beverages; health foods (supplements) such as tablets, capsules, granules, powders or functional foods; oral drugs such as tablets, capsules, granules, powders, syrups, extracts Etc. can be exemplified.

  The topical skin preparation or functional oral composition includes freesia plant bulbs or extracts thereof, as well as pharmaceuticals, quasi-drugs, skin cosmetics, hair cosmetics and washings, if necessary. Optional ingredients that are usually blended into the material such as water, oily ingredients, humectants, powders, pigments, emulsifiers, solubilizers, gelling agents, cleaning agents, UV absorbers, anti-inflammatory agents, thickeners, A pH adjuster, a chelating agent, a drug (medicinal component), a fragrance, a resin, a fungicide, a fungicide, an antioxidant, an alcohol, and the like can be appropriately blended. Furthermore, other moisturizers, anti-aging agents, whitening agents, antioxidants and slimming agents, plants other than plants of the genus Freesia or extracts thereof can be used within the range not impairing the effects of the present invention.

  Also in the case of oral compositions such as foods and drinks, there is no particular limitation in combination with various components that are usually used for oral use.

  The amount of freesia plant bulb or its extract to the topical skin preparation or functional oral composition can be adjusted depending on the type and purpose, but from the standpoint of efficacy and stability, In terms of solid content, 0.0001 to 10.0% by mass is preferable, more preferably 0.001 to 5.0% by mass, still more preferably 0.01 to 5% by mass, and still more preferably. It is 0.1-5 mass%.

  Examples of preparations for bulb extract of Freesia plants, test methods for evaluating moisturizing effect, anti-aging effect, antioxidant effect, whitening effect, anti-inflammatory effect, skin external preparation, functional food example Although described in more detail, the technical scope of the present invention is not limited thereby.

[Extract 1: Ethanol extract]
Freesia bulbs were dried and pulverized, 50 volume% ethanol of 20 times the mass of the sample was added and extracted at room temperature with stirring for 3 hours, and then insolubles were removed by filtration. After concentration under reduced pressure, freeze-drying was performed to obtain an extract.

[Extract 2: Hot water extract]
Freesia bulbs were dried and pulverized, purified water of 20 times the mass of the sample was added, and the mixture was extracted by heating at 120 ° C. for 20 minutes in an autoclave. An insoluble material was removed by suction filtration while maintaining a high temperature, and then lyophilized to obtain an extract.

  Using the above extract, the moisturizing effect, anti-aging effect, antioxidant effect, whitening effect, and anti-inflammatory effect of freesia (part: bulb) were evaluated. In addition, ** described in each evaluation result represents a significance probability of less than 1% (P <0.01) with respect to the significance probability P value in the t test.

<Moisturizing effect (hyaluronic acid production promoting action)>
Evaluation of the human dermal fibroblast hyaluronic acid production promoting effect of the freesia bulb extract was performed by the following method. Extract 1 was used as a sample.

Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS) was used as the seeding medium. After culturing for 24 hours, the culture medium was replaced with a DMEM medium supplemented with 0.5% by mass FBS to which the extract 1 was added so as to have each concentration shown in Table 1, and further cultured for 3 days. For the measurement of hyaluronic acid, a hyaluronic acid measurement kit manufactured by Seikagaku Biobusiness was used. The amount of protein in each well was measured using BCA Protein Assay Kit manufactured by PIERCE, and the amount of hyaluronic acid produced per unit amount of protein was determined. The evaluation results are shown in Table 1 as relative values with the amount of hyaluronic acid produced per unit protein in the control with no sample added as 100.

  As is apparent from Table 1, in the medium supplemented with freesia bulb extract, a significant human dermal fibroblast hyaluronic acid production promoting action was observed, and an excellent moisturizing effect was exhibited.

<Anti-aging effect (human dermal fibroblast activation)>
The human dermal fibroblast activation action of the freesia bulb extract was evaluated by the following method. Extract 2 was used as a sample.

Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. Dulbecco's modified Eagle medium (DMEM) supplemented with 1% by weight fetal bovine serum (FBS) was used as the seeding medium. After culturing for 24 hours, the culture medium was replaced with 1% by mass FBS-added DMEM medium to which the extract 2 was added so as to have each concentration shown in Table 2, and further cultured for 48 hours. Next, the MTT reagent adjusted with the culture medium so that it might become 400 micrograms / mL was added to the cell except the supernatant, and it culture | cultivated for about 2 hours. Finally, formazan produced in 2-propanol was extracted and the absorbance at 550 nm was measured. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated using the difference between the two measured values. In the evaluation, in addition to the sample culture solution, 1% by mass FBS-added DMEM medium was used as a control, and 5% by mass FBS-added DMEM medium was used as a positive control. The evaluation results are shown in Table 2 as relative values with the cell activation effect in the control with no sample added being taken as 100.

  As is clear from Table 2, a significant dermal fibroblast activation effect was observed in the medium supplemented with the freesia bulb extract.

<Anti-aging effect (human dermal fibroblast type I collagen production promoting action)>
Evaluation of human dermal fibroblast type I collagen production promoting action of the freesia bulb extract was carried out by the following method. Extract 2 was used as a sample.

Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS) was used as the seeding medium. After culturing for 24 hours, the culture medium was replaced with 0.5% by mass FBS-added DMEM medium to which the extract 2 was added so as to have each concentration shown in Table 3, and further cultured for 24 hours. The ELISA method was used for the quantification of type I collagen secreted into the culture supernatant. Finally, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt ( ABTS) and hydrogen peroxide were added and reacted, and then the absorbance at 405 nm was measured. The amount of protein in each well was measured with BCA Protein Assay Kit manufactured by PIERCE, and the amount of type I collagen produced per unit protein was determined. The evaluation results are shown in Table 3 as relative values when the amount of type I collagen produced per unit protein in the control with no sample added is defined as 100.

  As is clear from Table 3, in the medium supplemented with freesia bulb extract, a significant human dermal fibroblast type I collagen production promoting action was observed.

<Anti-aging effect (human epidermal keratinocyte activation action)>
Evaluation of the human epidermal keratinocyte activating effect of the freesia bulb extract was performed by the following method. Extract 2 was used as a sample.

Normal human epidermal keratinocytes NHEK were seeded in a 96-well microplate so as to be 2.0 × 10 ⁴ cells per well. Humedia-KG2 medium (Kurabo NHEK growth medium) was used as the seeding medium. After culturing for 24 hours, the medium was replaced with KG2 medium supplemented with extract 2 so that the concentrations shown in Table 4 were obtained, and further cultured for 24 hours. Next, the MTT reagent adjusted with the culture medium so that it might be set to 100 microgram / mL was added to the cell except the supernatant, and it culture | cultivated for about 1 hour. Finally, formazan produced in 2-propanol was extracted and the absorbance at 550 nm was measured. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated using the difference between the two measured values. The evaluation results are shown in Table 4 as relative values when the cell activation effect in the control with no sample added is taken as 100.

  As is clear from Table 4, in the medium supplemented with the freesia bulb extract, a significant keratinocyte activation effect was observed.

  As shown in Tables 2 to 4, the freesia bulb extract is excellent because it exhibits a human dermal fibroblast activation action, a human dermal fibroblast type I collagen production promotion action, and a human epidermal keratinocyte activation action. Exhibits anti-aging effects.

<Antioxidant effect (DPPH radical scavenging action)>
The DPPH radical scavenging action of the freesia bulb extract was evaluated by the following method. Extract 1 was used as a sample.

Add 100 μL of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) ethanol solution to 100 μL of the sample solution prepared by extracting Extract 1 with each concentration shown in Table 5 with 50 mass% ethanol. After mixing well, the mixture was allowed to stand in the dark at room temperature for 10 minutes, and the absorbance at 516 nm derived from the DPPH radical was measured. When the absorbance when no sample is added is (A) and the absorbance when the sample is added is (B), the DPPH radical elimination rate is defined by the following equation.
Erase rate = {1- (B) / (A)} × 100
The evaluation results are shown in Table 5.

  As apparent from Table 5, a significant DPPH radical scavenging action was observed in the freesia bulb extract.

<Antioxidant effect (superoxide anion scavenging action)>
Evaluation of SOD-like activity of freesia bulb extract (evaluation of superoxide anion scavenging action) was performed by the following method. Extract 1 was used as a sample.

Extract 1 was added to a HANK'S (+) solution in a concentration of 25 μL shown in Table 6 and 25 μL of a HANK'S (+) solution containing 0.25 mM WST-1 and 1 mM hypoxanthine. Was added. Furthermore, 25 μL (0.0075 Units) of xanthine oxidase was added, and after reacting at 37 ° C. for 15 minutes, the absorbance at 450 nm was measured. When the absorbance when no sample is added is (A) and the absorbance when the sample is added is (B), the superoxide anion elimination rate is defined by the following equation.
Erase rate (%) = {1- (B) / (A)} × 100
The evaluation results are shown in Table 6.

  As is clear from Table 6, a significant SOD-like activity (superoxide anion scavenging action) was observed in the freesia bulb extract.

  As shown in Tables 5 and 6, the freedia bulb extract exhibits an excellent antioxidant effect because it exhibits DPPH radical scavenging action and SOD-like activity (superoxide anion scavenging action).

<Whitening effect (tyrosinase activity inhibitory action)>
The human epidermal melanocyte tyrosinase activity inhibitory action of the freesia bulb extract was evaluated by the following method. Extract 2 was used as a sample.

Normal human epidermal melanocytes were seeded in a 96-well microplate so as to be 3.0 × 10 ⁴ cells per well. Medium 254S was used as the seeding medium. After culturing for 24 hours, the medium was replaced with Medium 254S to which the extract 2 was added so that the concentrations shown in Table 7 were obtained, followed by further culturing for 48 hours. Next, it was replaced with 75 μL of a phosphate buffer containing 1% by weight Triton-X to completely lyse the cells, and 50 μL was used as a crude enzyme solution. To the crude enzyme solution, 50 μL of a 0.05% by mass L-dopa-containing phosphate buffer as a substrate was added and allowed to stand at 37 ° C. for 2 hours. The absorbance at 405 nm was measured immediately after the addition of the substrate and at the end of the reaction, and the amount of dopamelanin produced was determined by introducing the difference between the two measured values into the following equation.
Amount of dopamelanin produced = {(405 nm value after reaction−405 nm value before reaction) −2.166} /5.238
In addition, the amount of protein in each well was measured with BCA Protein Assay Kit manufactured by PIERCE, and the amount of dopamelanin produced per unit protein amount was determined. The evaluation results are shown in Table 7 in comparison with the amount of dopamelanin produced per unit protein in the control with no sample added.

  As is clear from Table 7, a significant tyrosinase activity inhibitory action was observed in the medium supplemented with the freesia bulb extract.

<Whitening effect (melanin production inhibitory action)>
Evaluation of the B16 mouse melanoma cell melanin production inhibitory effect of the freesia bulb extract was performed by the following method. Extract 2 was used as a sample.

  B16 mouse melanoma cells (B16F0 cells) were seeded in a 90 mm dish so that there were 18000 cells per dish. Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS) was used as the seeding medium. After culturing for 24 hours, the medium was replaced with 5% by mass FBS-added DMEM medium to which the extract 2 was added so as to have each concentration shown in Table 8, and further cultured for 5 days. After completion of the culture, cells were peeled off by trypsin treatment, transferred to a 1.5 mL microtube, and centrifuged to obtain a cell precipitate. The obtained precipitate was visually determined based on the following determination table. In the evaluation, a 5% FBS-added DMEM medium containing 5% by mass FBS was used as a negative control, and a 5% FBS-added DMEM medium containing 50 mM sodium lactate was used as a positive control. These visual determination results were determined as determination 5 and determination 1, and used as an index for sample determination. As shown in Table 9, the visual judgment was evaluated in five stages. At the same time, a tissue solubilizer (trade name Solvable) was added to the precipitate, boiled, returned to room temperature, and the absorbance at 500 nm was measured with a spectrophotometer (HITACHI spectrophotometer U-3010) to determine the total amount of melanin. Asked. The evaluation results are shown in Table 8.

  As is clear from Table 8, in the medium supplemented with the freesia bulb extract, a significant B16 mouse melanoma cell melanin production inhibitory effect was observed.

  As shown in Tables 7 and 8, the freesia bulb extract exhibits excellent tyrosinase activity inhibitory action and B16 mouse melanoma cell melanin production inhibitory action, and thus exhibits an excellent whitening effect.

<Anti-inflammatory effect (hyaluronidase inhibitory action)>
The hyaluronidase inhibitory effect of freesia bulb extract was evaluated by the following method. Extract 1 was used as a sample.

Hyaluronic acid potassium salt (derived from human umbilical cord) was dissolved in 0.1 M phosphate buffer (pH 7.0) to a concentration of 0.9 mg / mL to obtain a substrate solution. Hyaluronidase (derived from bovine testis) was dissolved in 0.1 M phosphate buffer (pH 7.0) to 5300 unit / mL to obtain an enzyme solution. 0.1 mL of the sample solution and 0.03 mL of the enzyme solution prepared by extracting each extract 2 to the concentrations shown in Table 10 with a buffer solution were placed in a test tube and reacted at 37 ° C. for 20 minutes. Next, 0.06 mL of an activator was added and reacted at 37 ° C. for 20 minutes. Further, 0.15 mL of the substrate solution was added and reacted at 37 ° C. for 1 hour. 0.06 mL of 0.4 N NaOH was added, and ice-cooled immediately after the reaction was stopped. Boric acid buffer (pH 9.1) was added in an amount of 0.06 mL, boiled for 3 minutes, and further ice-cooled. After 2.0 mL of p-DABA solution was added and reacted at 37 ° C. for 20 minutes, the reaction solution was transferred to a 96-well microplate, and the absorbance at 585 nm was measured. As a control, a sample-free buffer solution was used. When the activity of hyaluronidase is inhibited, the degradation product N-acetylglucosamine (GlcNAc) decreases, and the absorbance by p-DABA decreases. Hyaluronidase inhibitory action is defined by the following equation. Inhibition rate (%) = (control absorbance−sample absorbance) / control absorbance × 100
Table 10 shows the evaluation results.

  As is apparent from Table 10, the freesia bulb extract has a significant hyaluronidase inhibitory action and exhibits an excellent anti-inflammatory effect.

  Then, the formulation example of the skin external preparation and functional oral composition which mix | blended the freesia bulb extract obtained by each said preparation method is shown.

[Example 1] Emulsion (1) Squalane 10.0 (mass%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 100 (11) Arginine (1% by weight aqueous solution) 20.0
(12) Extract 1 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After cooling, (11) and (12) are sequentially added at 40 ° C. and mixed uniformly.

[Example 2] Lotion (1) Ethanol 15.0 (mass%)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 100 (5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Extract 2 1.0
Production method: (2) and (3) are dissolved in (1). Further, after sequentially adding (4) to (8), the mixture is sufficiently stirred, and (9) is added and mixed uniformly.

[Example 3] Cream (1) Squalane 10.0 (mass%)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20 mass% aqueous solution) 15.0
(10) Remainder 100 (11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Extract 1 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. (11) is added and stirred, then cooled and (12) is added at 40 ° C. and mixed uniformly.

[Example 4] Cosmetic liquid (1) The balance (mass%) of purified water 100
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1% by weight aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (derived from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by mass aqueous solution) 2.0
(16) Extract 2 3.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. After cooling, add (15) at 50 ° C. and (16) at 40 ° C. and mix uniformly.

[Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (mass%)
(2) The balance made into purified water 100 (3) Sodium hydroxide (10 mass% aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Extract 2 0.5
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 1.0
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Example 6] Cleansing fee (1) Squalane 81.0 (mass%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Extract 1 4.0
Manufacturing method: (1) and (2) are uniformly dissolved. Add (3) to this and mix uniformly.

[Example 7] Face-wash foam (1) Stearic acid 16.0 (mass%)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 25.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) The balance which is made into purified water 100 (8) Extract 2 0.1
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the water phase components (5) to (7) are heated and dissolved at 80 ° C., and the oil phase components are uniformly mixed and stirred. After cooling, add (8) at 40 ° C. and mix uniformly.

[Example 8] Make-up base cream (1) Squalane 10.2 (% by mass)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) The balance which makes 100 purified water (8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Extract 1 3.0
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. After cooling, the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (mass%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Remainder water 100 (11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Extract 2 0.5
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the water phase components (7) to (10) are mixed, dissolved by heating at 75 ° C., the pigments (11) to (15) are added thereto, and the mixture is uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. After cooling, components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Example 10] Water-in-oil emollient cream (1) Liquid paraffin 34.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Extract 1 3.0
(11) The balance of 100 purified water (12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C, and gradually add to (4) heated to 50 ° C with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. with stirring, and emulsified with a homomixer. After cooling, add (12) at 40 ° C. and mix uniformly.

[Example 11] The balance (mass%) of pack (1) purified water 100
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 9.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Extract 2 1.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After evenly dissolving, add (4) and (5) and cool with stirring. Add (6) and (7) at 40 ° C. and mix uniformly.

[Example 12] Bath agent (1) Fragrance 0.3 (mass%)
(2) Extract 1 3.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 46.7
Production method: (1) to (4) are mixed uniformly.

[Example 13] Hair wax (1) Stearic acid 3.0 (mass%)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 100 (11) Extract 2 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. After cooling, the components (11) and (12) are added at 40 ° C. and mixed uniformly.

Example 14 Hair artic (1) Ethanol 50.0 (mass%)
(2) The balance of purified water 100 (3) Extract 1 3.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

Example 15 Tablet (1) Corn Starch 44.0 (mass%)
(2) Crystalline cellulose 100 (3) Carboxymethylcellulose calcium 5.0
(4) Silicic anhydride 0.5
(5) Magnesium stearate 0.5
(6) Extract 1 5.0
Production method: (1) to (6) are uniformly mixed, and compression-molded with a tableting machine to obtain one tablet of 200 mg.

[Example 16] Powder (1) Magnesium aluminate silicate 95.3 (% by mass)
(2) Carboxymethylcellulose calcium 100 The remainder (3) Extract 1 4.0
Production method: After the powders (1) to (3) are mixed, they are pulverized by a pulverizer and uniformly dispersed.

[Example 17] Candy (1) Sucrose 60.0 (mass%)
(2) Remaining as Minamata 100 (3) Extract 1 5.0
(4) Fragrance Appropriate amount Manufacturing method: (1) and (2) are heated, mixed and homogenized, cooled, components (3) and (4) are added at 70 ° C., mixed and homogenized, and then molded.

[Example 18] Drink (1) Aminoethylsulfonic acid 1000 mg
(2) Thiamine nitrate 10mg
(3) Riboflavin sodium phosphate 5mg
(4) Pyridoxine hydrochloride 10mg
(5) Anhydrous caffeine 50mg
(6) Citric acid 250mg
(7) D-sorbitol solution 8mg
(8) Extract 1 1000 mg
(9) Fragrance Trace amount (10) Purified water 100 mL of the remaining manufacturing method: (1) to (9) are sequentially added to (10) and homogenized.

  The skin external preparations shown in Examples 1 to 14 were compositions having a moisturizing action, an anti-aging action, an antioxidant action, a whitening action, and an anti-inflammatory action. The functional oral compositions shown in Examples 15 to 18 were compositions having a moisturizing action, an anti-aging action, an antioxidant action, a whitening action, and an anti-inflammatory action.

Claims (7)

  A moisturizing agent comprising as an active ingredient an extract of bulbs of Iridaceae Freesia genus (Freesia genus) plants.   An anti-aging agent comprising, as an active ingredient, an extract of bulbs of Iridaceae Freesia genus (Freesia genus) plants.   Antioxidant which uses the extract of bulb of Freesia genus (Freesia genus) plant as an active ingredient.   A whitening agent comprising, as an active ingredient, an extract of bulbs of Iridaceae Freesia genus (Freesia genus) plants.   An anti-inflammatory agent comprising, as an active ingredient, an extract of bulbs of Iridaceae Freesia genus (Freesia genus) plants.   An external preparation for skin containing, as an active ingredient, an extract of bulbs of Iridaceae Freesia genus (Freesia genus) plants.   The functional oral composition which uses the extract of the bulb of a Iridaceae Freesia genus (Freesia genus) plant as an active ingredient.