JP2011087571A - 細菌分析装置および細菌分析方法 - Google Patents
細菌分析装置および細菌分析方法 Download PDFInfo
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- JP2011087571A JP2011087571A JP2010191297A JP2010191297A JP2011087571A JP 2011087571 A JP2011087571 A JP 2011087571A JP 2010191297 A JP2010191297 A JP 2010191297A JP 2010191297 A JP2010191297 A JP 2010191297A JP 2011087571 A JP2011087571 A JP 2011087571A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 493
- 238000004458 analytical method Methods 0.000 title claims abstract description 158
- 238000001514 detection method Methods 0.000 claims abstract description 139
- 102000004190 Enzymes Human genes 0.000 claims abstract description 68
- 108090000790 Enzymes Proteins 0.000 claims abstract description 68
- 229940088598 enzyme Drugs 0.000 claims abstract description 68
- 108010014251 Muramidase Proteins 0.000 claims abstract description 63
- 102000016943 Muramidase Human genes 0.000 claims abstract description 63
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims abstract description 63
- 229960000274 lysozyme Drugs 0.000 claims abstract description 63
- 235000010335 lysozyme Nutrition 0.000 claims abstract description 63
- 239000004325 lysozyme Substances 0.000 claims abstract description 63
- 241000191940 Staphylococcus Species 0.000 claims abstract description 60
- 108090000988 Lysostaphin Proteins 0.000 claims abstract description 48
- 238000002360 preparation method Methods 0.000 claims abstract description 34
- 241000192125 Firmicutes Species 0.000 claims abstract description 19
- 210000002700 urine Anatomy 0.000 claims abstract description 19
- 238000005259 measurement Methods 0.000 claims description 231
- 230000001580 bacterial effect Effects 0.000 claims description 39
- 238000000034 method Methods 0.000 claims description 25
- 230000010365 information processing Effects 0.000 claims description 21
- 210000002421 cell wall Anatomy 0.000 claims description 3
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 claims description 2
- 239000012670 alkaline solution Substances 0.000 abstract description 9
- 239000000243 solution Substances 0.000 abstract description 9
- 241000295644 Staphylococcaceae Species 0.000 description 23
- 230000008569 process Effects 0.000 description 18
- 230000003287 optical effect Effects 0.000 description 14
- 239000012192 staining solution Substances 0.000 description 11
- 241000304886 Bacilli Species 0.000 description 9
- 238000004891 communication Methods 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 241000191967 Staphylococcus aureus Species 0.000 description 8
- 238000004590 computer program Methods 0.000 description 8
- 239000003085 diluting agent Substances 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 241000588724 Escherichia coli Species 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 238000000684 flow cytometry Methods 0.000 description 5
- 241000191963 Staphylococcus epidermidis Species 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 4
- 102000038379 digestive enzymes Human genes 0.000 description 4
- 108091007734 digestive enzymes Proteins 0.000 description 4
- 238000001802 infusion Methods 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 239000013049 sediment Substances 0.000 description 4
- 241000194032 Enterococcus faecalis Species 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 230000009257 reactivity Effects 0.000 description 3
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 2
- 241000194017 Streptococcus Species 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229960003085 meticillin Drugs 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 208000019206 urinary tract infection Diseases 0.000 description 2
- 241000194033 Enterococcus Species 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/12—Nitrate to nitrite reducing bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
- G01N2333/936—Hydrolases (3) acting on glycosyl compounds (3.2) acting on beta-1, 4 bonds between N-acetylmuramic acid and 2-acetyl-amino 2-deoxy-D-glucose, e.g. lysozyme
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
- G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
- G01N2333/96402—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from non-mammals
- G01N2333/96405—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from non-mammals in general
- G01N2333/96408—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from non-mammals in general with EC number
- G01N2333/96419—Metalloendopeptidases (3.4.24)
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2010191297A JP2011087571A (ja) | 2009-09-28 | 2010-08-27 | 細菌分析装置および細菌分析方法 |
| EP10179352A EP2308993A1 (en) | 2009-09-28 | 2010-09-24 | Bacteria analysis apparatus, bacteria analysis method and computer program product |
| CN2010102916476A CN102031224A (zh) | 2009-09-28 | 2010-09-26 | 细菌分析装置、细菌分析方法及其控制系统 |
| US12/891,567 US20110076716A1 (en) | 2009-09-28 | 2010-09-27 | Bacteria analysis apparatus, bacteria analysis method and computer program product |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009222645 | 2009-09-28 | ||
| JP2010191297A JP2011087571A (ja) | 2009-09-28 | 2010-08-27 | 細菌分析装置および細菌分析方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2011087571A true JP2011087571A (ja) | 2011-05-06 |
| JP2011087571A5 JP2011087571A5 (enExample) | 2013-10-10 |
Family
ID=43500178
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010191297A Pending JP2011087571A (ja) | 2009-09-28 | 2010-08-27 | 細菌分析装置および細菌分析方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20110076716A1 (enExample) |
| EP (1) | EP2308993A1 (enExample) |
| JP (1) | JP2011087571A (enExample) |
| CN (1) | CN102031224A (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USD670400S1 (en) * | 2011-05-05 | 2012-11-06 | Constitution Medical, Inc. | Blood analyzer |
| USD674912S1 (en) * | 2011-07-22 | 2013-01-22 | Constitution Medical, Inc. | Blood analyzer |
| USD705437S1 (en) * | 2012-12-28 | 2014-05-20 | Meso Scale Technologies, Llc. | Instrument |
| JP6948158B2 (ja) * | 2017-05-29 | 2021-10-13 | シスメックス株式会社 | 試料処理装置及び試料処理方法 |
| CN110592180A (zh) * | 2019-10-05 | 2019-12-20 | 天津市宝坻区人民医院 | 含有溶菌酶的尿素酶生化鉴定管 |
| CN114452429B (zh) * | 2021-12-20 | 2022-11-18 | 珠海格力电器股份有限公司 | 服饰的处理方法、装置、电子设备及储存介质 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1128099A (ja) * | 1997-05-12 | 1999-02-02 | Kikkoman Corp | 黄色ブドウ球菌の検出法 |
| WO2002101037A1 (en) * | 2001-05-31 | 2002-12-19 | Fuso Pharmaceutical Industries, Ltd. | Method of evaluating the function of phagocyte and utilization thereof |
| JP2003116595A (ja) * | 2001-10-05 | 2003-04-22 | Sysmex Corp | 薬剤感受性試験方法及び装置 |
| JP2005110629A (ja) * | 2003-10-10 | 2005-04-28 | Sysmex Corp | 細菌分析装置および方法 |
| US20050153370A1 (en) * | 2003-12-30 | 2005-07-14 | 3M Innovative Properties Company | Method of enhancing signal detection of cell-wall components of cells |
| JP2005198657A (ja) * | 2005-02-08 | 2005-07-28 | Fuso Pharmaceutical Industries Ltd | ヘリコバクターピロリ菌検出用プローブhp−34 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3529453B2 (ja) * | 1993-12-06 | 2004-05-24 | ミリポア・コーポレイション | 大腸菌群の選択的検出方法 |
| ATE366824T1 (de) * | 2001-05-31 | 2007-08-15 | Fuso Pharmaceutical Ind | Verbessertes verfahren zum nachweis und zur identifizierung eines eine infektion verursachenden mikroorganismus |
| JP2003093095A (ja) * | 2001-09-25 | 2003-04-02 | Hideo Tanaka | 大腸菌の特異的検出方法 |
| JP4484442B2 (ja) * | 2003-04-10 | 2010-06-16 | シスメックス株式会社 | 細菌測定方法と装置とプログラム |
-
2010
- 2010-08-27 JP JP2010191297A patent/JP2011087571A/ja active Pending
- 2010-09-24 EP EP10179352A patent/EP2308993A1/en not_active Withdrawn
- 2010-09-26 CN CN2010102916476A patent/CN102031224A/zh active Pending
- 2010-09-27 US US12/891,567 patent/US20110076716A1/en not_active Abandoned
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH1128099A (ja) * | 1997-05-12 | 1999-02-02 | Kikkoman Corp | 黄色ブドウ球菌の検出法 |
| WO2002101037A1 (en) * | 2001-05-31 | 2002-12-19 | Fuso Pharmaceutical Industries, Ltd. | Method of evaluating the function of phagocyte and utilization thereof |
| JP2003116595A (ja) * | 2001-10-05 | 2003-04-22 | Sysmex Corp | 薬剤感受性試験方法及び装置 |
| JP2005110629A (ja) * | 2003-10-10 | 2005-04-28 | Sysmex Corp | 細菌分析装置および方法 |
| US20050153370A1 (en) * | 2003-12-30 | 2005-07-14 | 3M Innovative Properties Company | Method of enhancing signal detection of cell-wall components of cells |
| JP2005198657A (ja) * | 2005-02-08 | 2005-07-28 | Fuso Pharmaceutical Industries Ltd | ヘリコバクターピロリ菌検出用プローブhp−34 |
Non-Patent Citations (1)
| Title |
|---|
| PLOS ONE, vol. Vol.4, Issue 5, JPN6014038164, 7 May 2009 (2009-05-07), pages 5470 - 1, ISSN: 0002894927 * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2308993A1 (en) | 2011-04-13 |
| CN102031224A (zh) | 2011-04-27 |
| US20110076716A1 (en) | 2011-03-31 |
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