JP2011083289A - 変異型ルシフェラーゼ - Google Patents

変異型ルシフェラーゼ Download PDF

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Publication number
JP2011083289A
JP2011083289A JP2011000791A JP2011000791A JP2011083289A JP 2011083289 A JP2011083289 A JP 2011083289A JP 2011000791 A JP2011000791 A JP 2011000791A JP 2011000791 A JP2011000791 A JP 2011000791A JP 2011083289 A JP2011083289 A JP 2011083289A
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Japan
Prior art keywords
luciferase
luciola
amino acid
residue
photinus pyralis
Prior art date
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JP2011000791A
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English (en)
Japanese (ja)
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JP2011083289A5 (de
Inventor
David James Squirrell
スクイレル，デイビツド・ジエイムズ
Melenie Jane Murphy
マーフイー，メレニー・ジエーン
Rachel Louise Price
プライス，レイチエル・ルイーズ
Peter John White
ホワイト，ピーター・ジヨン
Tara Louise Willey
ウイリー，テラ・ルイーズ
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3M Innovative Properties Co
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3M Innovative Properties Co
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Application filed by 3M Innovative Properties Co filed Critical 3M Innovative Properties Co
Publication of JP2011083289A publication Critical patent/JP2011083289A/ja
Publication of JP2011083289A5 publication Critical patent/JP2011083289A5/ja
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
JP2011000791A 1999-10-26 2011-01-05 変異型ルシフェラーゼ Withdrawn JP2011083289A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9925161.3A GB9925161D0 (en) 1999-10-26 1999-10-26 Novel enzyme
GBGB0016744.5A GB0016744D0 (en) 1999-10-26 2000-07-10 Novel enzyme

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2001533163A Division JP5154724B2 (ja) 1999-10-26 2000-10-26 変異型ルシフェラーゼ

Publications (2)

Publication Number Publication Date
JP2011083289A true JP2011083289A (ja) 2011-04-28
JP2011083289A5 JP2011083289A5 (de) 2012-09-13

Family

ID=10863292

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011000791A Withdrawn JP2011083289A (ja) 1999-10-26 2011-01-05 変異型ルシフェラーゼ

Country Status (6)

Country Link
US (2) US8669087B1 (de)
EP (2) EP1935980B1 (de)
JP (1) JP2011083289A (de)
GB (2) GB9925161D0 (de)
HK (1) HK1055443A1 (de)
ZA (1) ZA200203170B (de)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015223107A (ja) * 2014-05-27 2015-12-14 オリンパス株式会社 プロモーターアッセイ
WO2017221873A1 (ja) * 2016-06-21 2017-12-28 東亜ディーケーケー株式会社 変異型甲虫ルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型甲虫ルシフェラーゼの製造方法

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
WO2012061477A1 (en) 2010-11-02 2012-05-10 Promega Corporation Coelenterazine derivatives and methods of using same
US8669103B2 (en) 2010-11-02 2014-03-11 Promega Corporation Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
CN102191213B (zh) * 2011-04-14 2012-10-03 中国科学院武汉病毒研究所 一种萤火虫荧光素酶的编码基因及制备方法和应用
JP6588917B2 (ja) 2014-01-29 2019-10-09 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生細胞への適用のためのプロ基質
US9790537B2 (en) 2014-01-29 2017-10-17 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
WO2016040788A1 (en) 2014-09-11 2016-03-17 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
US11788068B2 (en) 2017-10-25 2023-10-17 Roche Molecular Systems, Inc. Modified/mutant bacterial luciferases

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JPH05244942A (ja) * 1991-06-27 1993-09-24 Kikkoman Corp 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、及び耐熱性ホタルルシフェラーゼの製造法
JPH08510387A (ja) * 1994-01-03 1996-11-05 プロメガ・コーポレイシヨン 突然変異体ルシフェラーゼ
JPH09510610A (ja) * 1994-03-23 1997-10-28 イギリス国 ルシフェラーゼ
WO1999014336A2 (en) * 1997-09-19 1999-03-25 Promega Corporation Thermostable luciferases and methods of production
JP2003512071A (ja) * 1999-10-26 2003-04-02 イギリス国 変異型ルシフェラーゼ

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JPS5782879A (en) 1980-11-12 1982-05-24 Mita Ind Co Ltd Photosensitive drum of electrostatic copier
US5583024A (en) 1985-12-02 1996-12-10 The Regents Of The University Of California Recombinant expression of Coleoptera luciferase
JPH088864B2 (ja) 1988-04-12 1996-01-31 キッコーマン株式会社 ルシフェラーゼ
US5049358A (en) 1988-09-30 1991-09-17 Miles Inc. Composition and test device for assaying for proteins
US5196524A (en) 1989-01-06 1993-03-23 Eli Lilly And Company Fusion reporter gene for bacterial luciferase
US5219737A (en) 1990-03-27 1993-06-15 Kikkoman Corporation Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase
US5480789A (en) 1991-04-01 1996-01-02 Florigene Europe B.V. Genetically transformed rose plants and methods for their production
US5229285A (en) 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
GB9301118D0 (en) 1993-01-21 1993-03-10 Secr Defence Enzyme linked assays
US6387675B1 (en) 1994-01-03 2002-05-14 Promega Corporation Mutant luciferases
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
GB2301592B (en) 1994-03-23 1998-06-17 Secr Defence Luciferases
DE69430327T2 (de) 1994-07-13 2002-10-31 Secr Defence Brit Mikrobiologisches testverfahren und reagenzien
US5670356A (en) 1994-12-12 1997-09-23 Promega Corporation Modified luciferase
CA2210354C (en) 1995-01-20 2008-01-08 The Secretary Of State For Defence Mutant luciferases
US5716851A (en) 1996-01-16 1998-02-10 Bayer Corporation Glass/cellulose as protein reagent
JPH09294600A (ja) 1996-04-26 1997-11-18 Kikkoman Corp 複数のプロモーター活性の測定法
JP2001504325A (ja) 1996-09-27 2001-04-03 マキシジェン，インコーポレイテッド 回帰的配列シャフリングおよび選択による遺伝子治療の最適化のための方法
GB9707486D0 (en) 1997-04-11 1997-05-28 Secr Defence Enzyme assays
US6074859A (en) 1997-07-08 2000-06-13 Kikkoman Corporation Mutant-type bioluminescent protein, and process for producing the mutant-type bioluminescent protein
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
US7083911B2 (en) 2001-02-16 2006-08-01 Promega Corporation Method for detection of ATP
US7183092B2 (en) * 2003-10-03 2007-02-27 Board Of Control Of Michigan Technological University Modified luciferase
JP5244942B2 (ja) 2011-04-25 2013-07-24 株式会社グレイプ 筋交い取付構造

Patent Citations (6)

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Publication number Priority date Publication date Assignee Title
JPH05244942A (ja) * 1991-06-27 1993-09-24 Kikkoman Corp 耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、及び耐熱性ホタルルシフェラーゼの製造法
JPH08510387A (ja) * 1994-01-03 1996-11-05 プロメガ・コーポレイシヨン 突然変異体ルシフェラーゼ
JPH09510610A (ja) * 1994-03-23 1997-10-28 イギリス国 ルシフェラーゼ
WO1999014336A2 (en) * 1997-09-19 1999-03-25 Promega Corporation Thermostable luciferases and methods of production
JP2003512071A (ja) * 1999-10-26 2003-04-02 イギリス国 変異型ルシフェラーゼ
JP5154724B2 (ja) * 1999-10-26 2013-02-27 プロメガ コーポレイション 変異型ルシフェラーゼ

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JPN6010037062; Ｂｉｏｃｈｅｍｉｓｔｒｙ，Ｖｏｌ．３２，Ｎｏ．５０（１９９３）ｐ．１３７９５-１３７９９ *
JPN6010037063; Ｂｉｏｃｈｅｍ．Ｊ．，Ｖｏｌ．３１９，Ｐｔ．２（１９９６）ｐ．３４３-３５０ *
JPN6011057384; Ｂｉｏｃｈｉｍ．Ｂｉｏｐｈｙｓ．Ａｃｔａ，Ｖｏｌ．１３３９，Ｎｏ．１（１９９７）ｐ．３９-５２ *
JPN7013001973; Ｌｉｐｍａｎ: 'Ｒａｐｉｄ　ａｎｄ　Ｓｅｎｓｉｔｉｖｅ　Ｐｒｏｔｅｉｎ　Ｓｉｍｉｌａｒｉｔｙ　Ｓｅａｒｃｈｅｓ' Ｓｃｉｅｎｃｅ ２２７, 1985, １４３５-１４４１ *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015223107A (ja) * 2014-05-27 2015-12-14 オリンパス株式会社 プロモーターアッセイ
WO2017221873A1 (ja) * 2016-06-21 2017-12-28 東亜ディーケーケー株式会社 変異型甲虫ルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型甲虫ルシフェラーゼの製造方法
JP2017225372A (ja) * 2016-06-21 2017-12-28 東亜ディーケーケー株式会社 変異型甲虫ルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型甲虫ルシフェラーゼの製造方法
US10851141B2 (en) 2016-06-21 2020-12-01 Dkk-Toa Corporation Mutant beetle luciferase, gene, recombinant vector, transformant, and method for preparing mutant beetle luciferase

Also Published As

Publication number Publication date
EP2284271B1 (de) 2013-09-11
GB9925161D0 (en) 1999-12-22
GB0016744D0 (en) 2000-08-30
EP2284271A3 (de) 2012-03-07
US8669087B1 (en) 2014-03-11
ZA200203170B (en) 2003-09-23
EP1935980B1 (de) 2013-07-17
US20140186918A1 (en) 2014-07-03
EP2284271A2 (de) 2011-02-16
EP1935980A1 (de) 2008-06-25
HK1055443A1 (en) 2004-01-09

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