JP2011024493A5 - - Google Patents
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- Publication number
- JP2011024493A5 JP2011024493A5 JP2009174234A JP2009174234A JP2011024493A5 JP 2011024493 A5 JP2011024493 A5 JP 2011024493A5 JP 2009174234 A JP2009174234 A JP 2009174234A JP 2009174234 A JP2009174234 A JP 2009174234A JP 2011024493 A5 JP2011024493 A5 JP 2011024493A5
- Authority
- JP
- Japan
- Prior art keywords
- pei
- solution
- nucleic acid
- polyethyleneimine
- introducing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009174234A JP5697123B2 (ja) | 2009-07-27 | 2009-07-27 | 酸性化ポリエチレンイミンを用いる細胞への核酸導入方法 |
| US12/752,188 US8507270B2 (en) | 2009-07-27 | 2010-04-01 | Method of transfecting cells with nucleic acids using acidified polyethylenimine |
| US13/936,259 US9249417B2 (en) | 2009-07-27 | 2013-07-08 | Reagent kit having acidified polvethvlendimine for introducting nucleic acids into cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009174234A JP5697123B2 (ja) | 2009-07-27 | 2009-07-27 | 酸性化ポリエチレンイミンを用いる細胞への核酸導入方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011024493A JP2011024493A (ja) | 2011-02-10 |
| JP2011024493A5 true JP2011024493A5 (enExample) | 2012-09-13 |
| JP5697123B2 JP5697123B2 (ja) | 2015-04-08 |
Family
ID=43497651
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009174234A Expired - Fee Related JP5697123B2 (ja) | 2009-07-27 | 2009-07-27 | 酸性化ポリエチレンイミンを用いる細胞への核酸導入方法 |
Country Status (2)
| Country | Link |
|---|---|
| US (2) | US8507270B2 (enExample) |
| JP (1) | JP5697123B2 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5697123B2 (ja) | 2009-07-27 | 2015-04-08 | 国立大学法人 千葉大学 | 酸性化ポリエチレンイミンを用いる細胞への核酸導入方法 |
| EP2338520A1 (de) | 2009-12-21 | 2011-06-29 | Ludwig Maximilians Universität | Konjugat mit Zielfindungsligand und dessen Verwendung |
| US10881682B2 (en) | 2015-08-26 | 2021-01-05 | International Business Machines Corporation | Therapeutic compositions comprising n-alkyl-hydroxy polymers |
| WO2017197471A1 (pt) * | 2016-05-19 | 2017-11-23 | Universidade Estadual De Campinas - Unicamp | Kit x-pei e método de transfecção celular |
| EP3510161A4 (en) | 2016-08-23 | 2020-04-22 | Akouos, Inc. | COMPOSITIONS AND METHODS FOR TREATING NON-AGE-ASSOCIATED HEARING DEFICIENCY IN A HUMAN SUBJECT |
| WO2018100089A1 (en) | 2016-12-01 | 2018-06-07 | Norbert Gretz | Means and methods for visualization of tissue structures |
| CN106943015A (zh) * | 2017-03-29 | 2017-07-14 | 广西喜爱家饮水设备有限公司 | 自动化饮水系统 |
| BR112019025792A2 (pt) | 2017-06-07 | 2020-07-07 | Spark Therapeutics, Inc. | agentes de aperfeiçoamento para transfecção de células melhoradas e/ou produção de vetor raav |
| JP7333930B2 (ja) * | 2019-01-07 | 2023-08-28 | 独立行政法人国立高等専門学校機構 | ミミズ細胞の保存方法及びミミズ培養細胞の形質転換方法 |
| WO2021168362A1 (en) | 2020-02-21 | 2021-08-26 | Akouos, Inc. | Compositions and methods for treating non-age-associated hearing impairment in a human subject |
| DE102020114183B4 (de) | 2020-05-27 | 2022-07-14 | Universität Leipzig Dezernat Forschung und Transfer | Mittel zur transfektion von nukleinsäuren in zellen |
| CN112695056A (zh) * | 2020-12-29 | 2021-04-23 | 苏州汇桢生物技术有限公司 | 一种提高瞬时转染效率的转染试剂及转染方法 |
| CN114561413B (zh) * | 2022-03-24 | 2023-09-29 | 新乡医学院 | 一种瞬时转染试剂及其应用 |
| EP4578952A1 (en) | 2022-08-22 | 2025-07-02 | FUJIFILM Corporation | Method for introducing nucleic acid into cells and application thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3996028B2 (ja) * | 2002-09-30 | 2007-10-24 | 株式会社日本触媒 | 蛋白質又はペプチドの細胞内導入方法 |
| CN1989213B (zh) * | 2004-07-28 | 2010-08-04 | 大日本油墨化学工业株式会社 | 有机无机复合涂膜及水性涂料组合物 |
| JP5697123B2 (ja) | 2009-07-27 | 2015-04-08 | 国立大学法人 千葉大学 | 酸性化ポリエチレンイミンを用いる細胞への核酸導入方法 |
-
2009
- 2009-07-27 JP JP2009174234A patent/JP5697123B2/ja not_active Expired - Fee Related
-
2010
- 2010-04-01 US US12/752,188 patent/US8507270B2/en not_active Expired - Fee Related
-
2013
- 2013-07-08 US US13/936,259 patent/US9249417B2/en active Active
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