JP2010531149A - 細胞懸濁液の処理 - Google Patents
細胞懸濁液の処理 Download PDFInfo
- Publication number
- JP2010531149A JP2010531149A JP2010513945A JP2010513945A JP2010531149A JP 2010531149 A JP2010531149 A JP 2010531149A JP 2010513945 A JP2010513945 A JP 2010513945A JP 2010513945 A JP2010513945 A JP 2010513945A JP 2010531149 A JP2010531149 A JP 2010531149A
- Authority
- JP
- Japan
- Prior art keywords
- oil
- composition
- suspension
- vacuum
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012545 processing Methods 0.000 title description 3
- 239000006285 cell suspension Substances 0.000 title 1
- 239000000203 mixture Substances 0.000 claims abstract description 128
- 239000002245 particle Substances 0.000 claims abstract description 64
- 238000000034 method Methods 0.000 claims abstract description 56
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 50
- 238000004113 cell culture Methods 0.000 claims abstract description 39
- 150000002632 lipids Chemical class 0.000 claims abstract description 35
- 241000894006 Bacteria Species 0.000 claims abstract description 27
- 239000004310 lactic acid Substances 0.000 claims abstract description 25
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 25
- 239000003921 oil Substances 0.000 claims description 75
- 235000019198 oils Nutrition 0.000 claims description 75
- 239000000725 suspension Substances 0.000 claims description 70
- 239000000463 material Substances 0.000 claims description 31
- 239000000843 powder Substances 0.000 claims description 21
- 235000019486 Sunflower oil Nutrition 0.000 claims description 11
- 239000002600 sunflower oil Substances 0.000 claims description 11
- 239000002562 thickening agent Substances 0.000 claims description 11
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 11
- 239000008158 vegetable oil Substances 0.000 claims description 11
- 239000002775 capsule Substances 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 10
- 235000013339 cereals Nutrition 0.000 claims description 9
- 238000009489 vacuum treatment Methods 0.000 claims description 9
- 235000005687 corn oil Nutrition 0.000 claims description 8
- 239000002285 corn oil Substances 0.000 claims description 8
- 239000003549 soybean oil Substances 0.000 claims description 8
- 235000012424 soybean oil Nutrition 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 7
- 239000004006 olive oil Substances 0.000 claims description 7
- 235000008390 olive oil Nutrition 0.000 claims description 7
- 230000001332 colony forming effect Effects 0.000 claims description 6
- 235000000832 Ayote Nutrition 0.000 claims description 5
- 240000004244 Cucurbita moschata Species 0.000 claims description 5
- 235000009854 Cucurbita moschata Nutrition 0.000 claims description 5
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 claims description 5
- 235000019487 Hazelnut oil Nutrition 0.000 claims description 5
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 5
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 5
- 244000028344 Primula vulgaris Species 0.000 claims description 5
- 235000016311 Primula vulgaris Nutrition 0.000 claims description 5
- 235000019774 Rice Bran oil Nutrition 0.000 claims description 5
- 239000010468 hazelnut oil Substances 0.000 claims description 5
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 5
- 235000015136 pumpkin Nutrition 0.000 claims description 5
- 239000008165 rice bran oil Substances 0.000 claims description 5
- 241000186000 Bifidobacterium Species 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 3
- 239000003674 animal food additive Substances 0.000 claims description 2
- 235000013373 food additive Nutrition 0.000 claims description 2
- 239000002778 food additive Substances 0.000 claims description 2
- 238000003860 storage Methods 0.000 abstract description 15
- 210000004027 cell Anatomy 0.000 description 75
- 239000001993 wax Substances 0.000 description 29
- 239000007789 gas Substances 0.000 description 25
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 22
- 238000009472 formulation Methods 0.000 description 15
- 244000057717 Streptococcus lactis Species 0.000 description 12
- 235000014897 Streptococcus lactis Nutrition 0.000 description 12
- 230000000694 effects Effects 0.000 description 11
- 150000001875 compounds Chemical class 0.000 description 9
- GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 9
- 244000005700 microbiome Species 0.000 description 9
- 229920001592 potato starch Polymers 0.000 description 9
- 230000000529 probiotic effect Effects 0.000 description 9
- 239000000377 silicon dioxide Substances 0.000 description 9
- 235000010384 tocopherol Nutrition 0.000 description 9
- 229960001295 tocopherol Drugs 0.000 description 9
- 229930003799 tocopherol Natural products 0.000 description 9
- 239000011732 tocopherol Substances 0.000 description 9
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 9
- 235000012239 silicon dioxide Nutrition 0.000 description 8
- 239000006041 probiotic Substances 0.000 description 7
- 235000018291 probiotics Nutrition 0.000 description 7
- 210000005253 yeast cell Anatomy 0.000 description 7
- 230000000813 microbial effect Effects 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 5
- 239000008176 lyophilized powder Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 229940009289 bifidobacterium lactis Drugs 0.000 description 4
- 235000013365 dairy product Nutrition 0.000 description 4
- 210000001236 prokaryotic cell Anatomy 0.000 description 4
- 239000007858 starting material Substances 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 229910002012 Aerosil® Inorganic materials 0.000 description 3
- 241000235349 Ascomycota Species 0.000 description 3
- 241001149698 Lipomyces Species 0.000 description 3
- 241000235648 Pichia Species 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 3
- 241000194020 Streptococcus thermophilus Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 239000007903 gelatin capsule Substances 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000000859 sublimation Methods 0.000 description 3
- 230000008022 sublimation Effects 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000186216 Corynebacterium Species 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 241000186660 Lactobacillus Species 0.000 description 2
- 244000199866 Lactobacillus casei Species 0.000 description 2
- 235000013958 Lactobacillus casei Nutrition 0.000 description 2
- 240000002605 Lactobacillus helveticus Species 0.000 description 2
- 235000013967 Lactobacillus helveticus Nutrition 0.000 description 2
- 241000192130 Leuconostoc mesenteroides Species 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 241000187747 Streptomyces Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- YZXBAPSDXZZRGB-DOFZRALJSA-N arachidonic acid Chemical compound CCCCC\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O YZXBAPSDXZZRGB-DOFZRALJSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 230000006860 carbon metabolism Effects 0.000 description 2
- 235000013351 cheese Nutrition 0.000 description 2
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Chemical compound CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 229920001903 high density polyethylene Polymers 0.000 description 2
- 239000004700 high-density polyethylene Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229940054346 lactobacillus helveticus Drugs 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- WWZKQHOCKIZLMA-UHFFFAOYSA-N octanoic acid Chemical compound CCCCCCCC(O)=O WWZKQHOCKIZLMA-UHFFFAOYSA-N 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 230000009105 vegetative growth Effects 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 235000013618 yogurt Nutrition 0.000 description 2
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 241001019659 Acremonium <Plectosphaerellaceae> Species 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 229910002016 Aerosil® 200 Inorganic materials 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 239000005632 Capric acid (CAS 334-48-5) Substances 0.000 description 1
- 239000005635 Caprylic acid (CAS 124-07-2) Substances 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 244000180278 Copernicia prunifera Species 0.000 description 1
- 235000010919 Copernicia prunifera Nutrition 0.000 description 1
- 241000235035 Debaryomyces Species 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 241000194031 Enterococcus faecium Species 0.000 description 1
- 241001465321 Eremothecium Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 241000223198 Humicola Species 0.000 description 1
- 241000024378 Ilybius subtilis Species 0.000 description 1
- 241000186673 Lactobacillus delbrueckii Species 0.000 description 1
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 1
- 241000194036 Lactococcus Species 0.000 description 1
- 241000194041 Lactococcus lactis subsp. lactis Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 239000005639 Lauric acid Substances 0.000 description 1
- 241000192132 Leuconostoc Species 0.000 description 1
- 229920000057 Mannan Polymers 0.000 description 1
- 241000235395 Mucor Species 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 208000012868 Overgrowth Diseases 0.000 description 1
- 239000005662 Paraffin oil Substances 0.000 description 1
- 235000019483 Peanut oil Nutrition 0.000 description 1
- 241000192001 Pediococcus Species 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241000235003 Saccharomycopsis Species 0.000 description 1
- 241000235346 Schizosaccharomyces Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 235000014969 Streptococcus diacetilactis Nutrition 0.000 description 1
- 241001494489 Thielavia Species 0.000 description 1
- 241001149964 Tolypocladium Species 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 235000011054 acetic acid Nutrition 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 230000002528 anti-freeze Effects 0.000 description 1
- 239000007798 antifreeze agent Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 229940114079 arachidonic acid Drugs 0.000 description 1
- 235000021342 arachidonic acid Nutrition 0.000 description 1
- 230000001680 brushing effect Effects 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- 239000004203 carnauba wax Substances 0.000 description 1
- 235000013869 carnauba wax Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000003240 coconut oil Substances 0.000 description 1
- 235000019864 coconut oil Nutrition 0.000 description 1
- 239000012612 commercial material Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000008157 edible vegetable oil Substances 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000010462 extra virgin olive oil Substances 0.000 description 1
- 235000021010 extra-virgin olive oil Nutrition 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- -1 fatty acid lipid Chemical class 0.000 description 1
- 239000006052 feed supplement Substances 0.000 description 1
- 235000021105 fermented cheese Nutrition 0.000 description 1
- 235000021001 fermented dairy product Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000021323 fish oil Nutrition 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 150000002334 glycols Chemical class 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 239000007902 hard capsule Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000008172 hydrogenated vegetable oil Substances 0.000 description 1
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 description 1
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 238000010907 mechanical stirring Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000004200 microcrystalline wax Substances 0.000 description 1
- 235000019808 microcrystalline wax Nutrition 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 229960002446 octanoic acid Drugs 0.000 description 1
- 239000012053 oil suspension Substances 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 235000021313 oleic acid Nutrition 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000000312 peanut oil Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 239000012178 vegetable wax Substances 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200700939 | 2007-06-28 | ||
| PCT/EP2008/058322 WO2009000924A1 (en) | 2007-06-28 | 2008-06-27 | Treatment of cell suspension |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010531149A true JP2010531149A (ja) | 2010-09-24 |
| JP2010531149A5 JP2010531149A5 (enExample) | 2011-08-11 |
Family
ID=39858758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010513945A Pending JP2010531149A (ja) | 2007-06-28 | 2008-06-27 | 細胞懸濁液の処理 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20100183768A1 (enExample) |
| EP (1) | EP2173856A1 (enExample) |
| JP (1) | JP2010531149A (enExample) |
| WO (1) | WO2009000924A1 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013537037A (ja) * | 2010-08-26 | 2013-09-30 | ダウ グローバル テクノロジーズ エルエルシー | プロバイオティックスの貯蔵安定性を高める方法 |
| JPWO2019172112A1 (ja) * | 2018-03-06 | 2021-02-18 | ゼノアックリソース株式会社 | 細胞凍結保存用溶液およびその利用 |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010096564A2 (en) * | 2009-02-19 | 2010-08-26 | Probiohealth Llc | Dietary supplements containing polyunsaturated omega-3 fatty acids and probiotic bacteria with potential gastrointestinal and dermatological benefits |
| IT1400821B1 (it) * | 2009-03-09 | 2013-07-02 | Probiotical Spa | Sospensione oleosa contenente batteri probiotici per uso pediatrico |
| ITUD20120064A1 (it) * | 2012-04-17 | 2013-10-18 | Biofarma S P A | Prodotto confezionato comprendente un integratore alimentare per l'infanzia |
| CN105707897A (zh) * | 2016-02-02 | 2016-06-29 | 上海交大昂立股份有限公司 | 一种益生菌脂性混悬液滴剂及其制备方法 |
| WO2020239761A1 (en) * | 2019-05-28 | 2020-12-03 | Chr. Hansen A/S | Process for producing a fermented milk product with an enhanced level of probiotics |
| CN113575756A (zh) * | 2021-07-09 | 2021-11-02 | 内蒙古普泽动保生物技术有限公司 | 一种含益生菌的油性悬浮液及其制备方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10287573A (ja) * | 1997-04-14 | 1998-10-27 | Dr A Tosi Farmaceut Srl | トランス粘膜投与に適したラクトバシラスよりなる製薬組成物 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK242083D0 (da) * | 1983-05-27 | 1983-05-27 | Hansens Chr Bio Syst | Vaginalkapsler |
| IT1231934B (it) * | 1989-09-04 | 1992-01-15 | Biozeta Srl | Composizioni a base di tessuti vegetali od animali e procedimento per la loro preparazione. |
| CA2127392C (en) * | 1993-07-08 | 2008-05-27 | Hideki Sunohara | Process for producing capsule and capsule obtained thereby |
| JP3985906B2 (ja) * | 1996-07-23 | 2007-10-03 | ビユーラー・アクチエンゲゼルシヤフト | 顆粒製品に油を引くための装置 |
| EP0862863B9 (en) * | 1997-01-09 | 2009-08-12 | Societe Des Produits Nestle S.A. | Cereal product containing probiotics |
| CZ287989B6 (cs) * | 1998-03-20 | 2001-03-14 | Medipharm Cz, S. R. O. | Perorální přípravek k prevenci a léčbě infekčních gastroenteritid prasat |
| US6159504A (en) * | 1999-01-11 | 2000-12-12 | Kitii Corporation, Ltd. | Core substance-containing calcium microparticles and methods for producing the same |
| US7153472B1 (en) * | 2000-11-22 | 2006-12-26 | Quadrant Drug Delivery Limited | Preservation and formulation of bioactive materials for storage and delivery in hydrophobic carriers |
| US20050106132A1 (en) * | 2003-08-14 | 2005-05-19 | Porubcan Randolph S. | Growth promoting prebiotic for lactobacillus dietary supplements |
| ATE382677T1 (de) * | 2005-02-18 | 2008-01-15 | Gnosis Spa | Verfahren zur herstellung von hefe-mikrokapseln |
-
2008
- 2008-06-27 US US12/664,957 patent/US20100183768A1/en not_active Abandoned
- 2008-06-27 WO PCT/EP2008/058322 patent/WO2009000924A1/en not_active Ceased
- 2008-06-27 EP EP08774481A patent/EP2173856A1/en not_active Withdrawn
- 2008-06-27 JP JP2010513945A patent/JP2010531149A/ja active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH10287573A (ja) * | 1997-04-14 | 1998-10-27 | Dr A Tosi Farmaceut Srl | トランス粘膜投与に適したラクトバシラスよりなる製薬組成物 |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013537037A (ja) * | 2010-08-26 | 2013-09-30 | ダウ グローバル テクノロジーズ エルエルシー | プロバイオティックスの貯蔵安定性を高める方法 |
| JPWO2019172112A1 (ja) * | 2018-03-06 | 2021-02-18 | ゼノアックリソース株式会社 | 細胞凍結保存用溶液およびその利用 |
| JP2023076609A (ja) * | 2018-03-06 | 2023-06-01 | ゼノジェンファーマ株式会社 | 細胞凍結保存用溶液およびその利用 |
| JP7672127B2 (ja) | 2018-03-06 | 2025-05-07 | ゼノジェンファーマ株式会社 | 細胞凍結保存用溶液およびその利用 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2173856A1 (en) | 2010-04-14 |
| WO2009000924A1 (en) | 2008-12-31 |
| US20100183768A1 (en) | 2010-07-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2010531149A (ja) | 細胞懸濁液の処理 | |
| AU2014286177B2 (en) | A method of freeze-drying encapsulated cells, freeze-dried encapsulated cells, compositions containing freeze-dried encapsulated cells and uses of such cells and compositions | |
| AU2008325308B2 (en) | Agent stabilisation process and product | |
| US20240150706A1 (en) | Formulations of microencapsulated microbial culture with high storage stability | |
| CN105996051A (zh) | 包含乳酸菌和保护剂的新型组合物 | |
| US20230323332A1 (en) | Microencapsulation of microbial culture using octenyl succinic anhydride starch-chitosan complex coacervate | |
| EP2318511A1 (en) | Bacterial composition | |
| JP2017529099A (ja) | 生存プロバイオティック細菌を含む軟質ゲルカプセルの改善された生産方法、及び長い保存可能期間を有する生存プロバイオティック細菌を含む軟質ゲルカプセル | |
| US10532076B2 (en) | Probiotic anhydrous fatty foodstuffs and methods of making same | |
| AU2019250114A1 (en) | Pre-packaged coffee product | |
| US20250215415A1 (en) | Formulations of microencapsulated microbial culture with high storage stability | |
| CN116507718A (zh) | 稳定的乳酸菌组合物 | |
| EP4440338A1 (en) | Improved stability of microbial compositions, and manufacturing methods therefore | |
| US20240023568A1 (en) | Fat encapsulated microbial cultures | |
| JP2012056921A (ja) | 生菌製剤 | |
| TW200846466A (en) | Probiotic powder composition and product thereof | |
| EP4440340A1 (en) | Compositions for increased stability of bacteria | |
| Ouwehand et al. | Probiotics: from strain to product | |
| EP4440341A1 (en) | Fat and wax microencapsulated ambient stable bacteria and probiotics | |
| CN114874935A (zh) | 一种高活性的益生菌制剂组合物及其制备方法 | |
| JP2007053981A (ja) | 粉末状食品材料とそれを用いた食品 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20110624 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20110624 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20120620 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20130702 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20130926 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20131003 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20140304 |