JP2010527580A5 - - Google Patents
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- JP2010527580A5 JP2010527580A5 JP2009532723A JP2009532723A JP2010527580A5 JP 2010527580 A5 JP2010527580 A5 JP 2010527580A5 JP 2009532723 A JP2009532723 A JP 2009532723A JP 2009532723 A JP2009532723 A JP 2009532723A JP 2010527580 A5 JP2010527580 A5 JP 2010527580A5
- Authority
- JP
- Japan
- Prior art keywords
- group
- alkenyl
- alkyl
- independently selected
- groups
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 125000003342 alkenyl group Chemical group 0.000 claims 32
- 125000000217 alkyl group Chemical group 0.000 claims 30
- 125000003118 aryl group Chemical group 0.000 claims 22
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 claims 20
- 125000000304 alkynyl group Chemical group 0.000 claims 16
- 125000005843 halogen group Chemical group 0.000 claims 14
- 125000003729 nucleotide group Chemical group 0.000 claims 13
- 239000002773 nucleotide Substances 0.000 claims 11
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 10
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 10
- 108091034117 Oligonucleotide Proteins 0.000 claims 10
- 125000004104 aryloxy group Chemical group 0.000 claims 10
- 108091033319 polynucleotide Proteins 0.000 claims 9
- 102000040430 polynucleotide Human genes 0.000 claims 9
- 239000002157 polynucleotide Substances 0.000 claims 9
- 125000003545 alkoxy group Chemical group 0.000 claims 6
- 125000003282 alkyl amino group Chemical group 0.000 claims 6
- 125000001033 ether group Chemical group 0.000 claims 6
- 125000000896 monocarboxylic acid group Chemical group 0.000 claims 6
- 238000000034 method Methods 0.000 claims 5
- 102200120949 rs199517715 Human genes 0.000 claims 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 4
- 239000002202 Polyethylene glycol Substances 0.000 claims 4
- 125000003158 alcohol group Chemical group 0.000 claims 4
- 229920001223 polyethylene glycol Polymers 0.000 claims 4
- 125000000101 thioether group Chemical group 0.000 claims 4
- 102100034343 Integrase Human genes 0.000 claims 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 3
- 108010006785 Taq Polymerase Proteins 0.000 claims 3
- 241000589596 Thermus Species 0.000 claims 3
- 239000003153 chemical reaction reagent Substances 0.000 claims 3
- 238000001668 nucleic acid synthesis Methods 0.000 claims 3
- -1 αDNA polymerase Proteins 0.000 claims 3
- KXDHJXZQYSOELW-UHFFFAOYSA-N Carbamic acid Chemical group NC(O)=O KXDHJXZQYSOELW-UHFFFAOYSA-N 0.000 claims 2
- 125000003172 aldehyde group Chemical group 0.000 claims 2
- 125000005233 alkylalcohol group Chemical group 0.000 claims 2
- 125000003368 amide group Chemical group 0.000 claims 2
- 125000003277 amino group Chemical group 0.000 claims 2
- 229960002685 biotin Drugs 0.000 claims 2
- 235000020958 biotin Nutrition 0.000 claims 2
- 239000011616 biotin Substances 0.000 claims 2
- 230000000903 blocking effect Effects 0.000 claims 2
- 125000002843 carboxylic acid group Chemical group 0.000 claims 2
- 125000004185 ester group Chemical group 0.000 claims 2
- 125000000623 heterocyclic group Chemical group 0.000 claims 2
- 125000001165 hydrophobic group Chemical group 0.000 claims 2
- 125000005462 imide group Chemical group 0.000 claims 2
- 125000000468 ketone group Chemical group 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 108010017826 DNA Polymerase I Proteins 0.000 claims 1
- 102000004594 DNA Polymerase I Human genes 0.000 claims 1
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims 1
- 101710200526 DNA polymerase 2 Proteins 0.000 claims 1
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 claims 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 101001066878 Homo sapiens Polyribonucleotide nucleotidyltransferase 1, mitochondrial Proteins 0.000 claims 1
- 241000192041 Micrococcus Species 0.000 claims 1
- 102000002681 Polyribonucleotide nucleotidyltransferase Human genes 0.000 claims 1
- 102000009572 RNA Polymerase II Human genes 0.000 claims 1
- 108010009460 RNA Polymerase II Proteins 0.000 claims 1
- 108010065868 RNA polymerase SP6 Proteins 0.000 claims 1
- 101710137500 T7 RNA polymerase Proteins 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 108010028263 bacteriophage T3 RNA polymerase Proteins 0.000 claims 1
- 239000011942 biocatalyst Substances 0.000 claims 1
- 238000010348 incorporation Methods 0.000 claims 1
- 125000006239 protecting group Chemical group 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 0 CN(C(N1)=*)N=C(*)C1=* Chemical compound CN(C(N1)=*)N=C(*)C1=* 0.000 description 18
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US11/583,605 US7928207B2 (en) | 2004-06-28 | 2006-10-18 | Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides |
| PCT/EP2007/009019 WO2008046609A2 (en) | 2006-10-18 | 2007-10-18 | Synthesis and compositions of nucleic acids comprising 2'-terminator nucleotides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2010527580A JP2010527580A (ja) | 2010-08-19 |
| JP2010527580A5 true JP2010527580A5 (https=) | 2010-12-02 |
Family
ID=39078489
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009532723A Pending JP2010527580A (ja) | 2006-10-18 | 2007-10-18 | ２’−ターミネーター・ヌクレオチドを含む核酸の合成及び組成物 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7928207B2 (https=) |
| EP (1) | EP2195327A2 (https=) |
| JP (1) | JP2010527580A (https=) |
| CN (1) | CN102083846A (https=) |
| CA (1) | CA2666555A1 (https=) |
| WO (1) | WO2008046609A2 (https=) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| US7902345B2 (en) | 2006-12-05 | 2011-03-08 | Sequenom, Inc. | Detection and quantification of biomolecules using mass spectrometry |
| WO2009032781A2 (en) | 2007-08-29 | 2009-03-12 | Sequenom, Inc. | Methods and compositions for universal size-specific polymerase chain reaction |
| US10150990B2 (en) | 2008-04-21 | 2018-12-11 | Roche Molecular Systems, Inc. | Ribonucleotide tag nucleic acid detection |
| ES2384681T3 (es) * | 2008-08-12 | 2012-07-10 | F. Hoffmann-La Roche Ag | Extensión de cebadores correctora |
| MD20140044A2 (ro) | 2011-11-11 | 2014-08-31 | Pfizer Inc. | 2-Tiopirimidinone şi utilizarea lor pentru tratamentul afecţiunilor cardiovasculare |
| CN103266103B (zh) * | 2012-11-08 | 2016-08-31 | 天津精耐特基因生物技术有限公司 | 用于扩增核糖核酸的方法及分析该方法中rna或dna模板的方法 |
| US11331643B2 (en) | 2013-04-02 | 2022-05-17 | Molecular Assemblies, Inc. | Reusable initiators for synthesizing nucleic acids |
| US10683536B2 (en) | 2013-04-02 | 2020-06-16 | Molecular Assemblies, Inc. | Reusable initiators for synthesizing nucleic acids |
| US8808989B1 (en) * | 2013-04-02 | 2014-08-19 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
| US11384377B2 (en) | 2013-04-02 | 2022-07-12 | Molecular Assemblies, Inc. | Reusable initiators for synthesizing nucleic acids |
| US9771613B2 (en) | 2013-04-02 | 2017-09-26 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acid |
| US9279149B2 (en) | 2013-04-02 | 2016-03-08 | Molecular Assemblies, Inc. | Methods and apparatus for synthesizing nucleic acids |
| US9856524B2 (en) * | 2014-12-04 | 2018-01-02 | Shaofeng Ding | Lyophilized integrated composition for storage and manipulation of pyrophosphorolysis activated polymerization |
| AU2016257179A1 (en) | 2015-05-05 | 2017-11-02 | Pfizer Inc. | 2-thiopyrimidinones |
| US10465242B2 (en) | 2016-07-14 | 2019-11-05 | University Of Utah Research Foundation | Multi-sequence capture system |
| US11921049B2 (en) * | 2018-11-09 | 2024-03-05 | Shaofeng Ding | Pyrophosphorolysis activated fluorescence to measure PAP amplification of nucleic acid |
| EP3969593A1 (en) * | 2019-05-15 | 2022-03-23 | Massachusetts Institute of Technology | Optimization of circular single stranded dna using m13 phage |
| CN116024320A (zh) * | 2022-07-13 | 2023-04-28 | 上海翔琼生物技术有限公司 | 一种用于检测核酸的荧光定量pcr方法 |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1219824A (en) * | 1981-04-17 | 1987-03-31 | David C. Ward | Modified nucleotides and methods of preparing and using same |
| CA1340806C (en) * | 1986-07-02 | 1999-11-02 | James Merrill Prober | Method, system and reagents for dna sequencing |
| JPH0778074B2 (ja) * | 1989-09-29 | 1995-08-23 | 堺化学工業株式会社 | ヌクレオシド―２’，５’‐及び３’，５’‐二リン酸の製造方法 |
| US5859221A (en) * | 1990-01-11 | 1999-01-12 | Isis Pharmaceuticals, Inc. | 2'-modified oligonucleotides |
| US5914396A (en) * | 1990-01-11 | 1999-06-22 | Isis Pharmaceuticals, Inc. | 2'-O-modified nucleosides and phosphoramidites |
| US5660985A (en) * | 1990-06-11 | 1997-08-26 | Nexstar Pharmaceuticals, Inc. | High affinity nucleic acid ligands containing modified nucleotides |
| CA2153387A1 (en) * | 1993-01-07 | 1994-07-21 | Hubert Koester | Dna sequencing by mass spectrometry |
| US5532130A (en) | 1993-07-20 | 1996-07-02 | Dyad Pharmaceutical Corporation | Methods and compositions for sequence-specific hybridization of RNA by 2'-5' oligonucleotides |
| DE69735445T2 (de) * | 1996-12-10 | 2006-08-10 | Sequenom, Inc., San Diego | Abspaltbare, nicht-flüchtige moleküle zur massenmarkierung |
| US6147200A (en) * | 1999-08-19 | 2000-11-14 | Isis Pharmaceuticals, Inc. | 2'-O-acetamido modified monomers and oligomers |
| EP1409731A2 (en) * | 2001-07-03 | 2004-04-21 | ISIS Pharmaceuticals, Inc. | Methods of modulating pharmacokinetics of oligonucleotides |
| JP3827629B2 (ja) | 2002-08-30 | 2006-09-27 | 住友大阪セメント株式会社 | 光変調器 |
| US7572581B2 (en) | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| US7947817B2 (en) * | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| AU2005250239A1 (en) | 2004-06-02 | 2005-12-15 | Alere San Diego, Inc. | 2'-nitrobenzyl-modified ribonucleotides |
| US7745125B2 (en) | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| JP4753066B2 (ja) | 2005-06-29 | 2011-08-17 | 独立行政法人産業技術総合研究所 | イオン複合型粘土組成物およびその固化物 |
| CN101346472B (zh) | 2005-12-21 | 2013-04-24 | 霍夫曼—拉罗奇有限公司 | 用可逆2’-修饰的核苷酸进行的序列测定和基因分型 |
-
2006
- 2006-10-18 US US11/583,605 patent/US7928207B2/en not_active Expired - Fee Related
-
2007
- 2007-10-18 CN CN200780046819XA patent/CN102083846A/zh active Pending
- 2007-10-18 CA CA002666555A patent/CA2666555A1/en not_active Abandoned
- 2007-10-18 WO PCT/EP2007/009019 patent/WO2008046609A2/en not_active Ceased
- 2007-10-18 JP JP2009532723A patent/JP2010527580A/ja active Pending
- 2007-10-18 EP EP07819087A patent/EP2195327A2/en not_active Withdrawn
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