JP2010514429A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2010514429A5 JP2010514429A5 JP2009543399A JP2009543399A JP2010514429A5 JP 2010514429 A5 JP2010514429 A5 JP 2010514429A5 JP 2009543399 A JP2009543399 A JP 2009543399A JP 2009543399 A JP2009543399 A JP 2009543399A JP 2010514429 A5 JP2010514429 A5 JP 2010514429A5
- Authority
- JP
- Japan
- Prior art keywords
- mrsa
- probe
- fluorescent moiety
- sample
- primers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 claims 41
- 230000003321 amplification Effects 0.000 claims 15
- 238000003199 nucleic acid amplification method Methods 0.000 claims 15
- 238000000034 method Methods 0.000 claims 13
- 150000007523 nucleic acids Chemical group 0.000 claims 11
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 9
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 8
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 claims 4
- 241000191967 Staphylococcus aureus Species 0.000 claims 4
- 229960003085 meticillin Drugs 0.000 claims 4
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 claims 2
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 claims 2
- 230000015572 biosynthetic process Effects 0.000 claims 2
- 210000000349 chromosome Anatomy 0.000 claims 2
- 238000001514 detection method Methods 0.000 claims 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical group O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 2
- 238000002372 labelling Methods 0.000 claims 2
- 238000002844 melting Methods 0.000 claims 2
- 230000008018 melting Effects 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 239000012472 biological sample Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000001124 body fluid Anatomy 0.000 claims 1
- 239000010839 body fluid Substances 0.000 claims 1
- 210000001217 buttock Anatomy 0.000 claims 1
- 230000002759 chromosomal effect Effects 0.000 claims 1
- 239000013068 control sample Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 108010068698 spleen exonuclease Proteins 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
- 210000002700 urine Anatomy 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US88279906P | 2006-12-29 | 2006-12-29 | |
| US60/882,799 | 2006-12-29 | ||
| EP07008206 | 2007-04-23 | ||
| EP07008206.0 | 2007-04-23 | ||
| PCT/EP2007/011458 WO2008080620A1 (en) | 2006-12-29 | 2007-12-28 | Methods for detecting methicillin-resistant s. aureus as well as primers, probes and kits for the same |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010514429A JP2010514429A (ja) | 2010-05-06 |
| JP2010514429A5 true JP2010514429A5 (https=) | 2010-11-25 |
| JP5203387B2 JP5203387B2 (ja) | 2013-06-05 |
Family
ID=38473002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009543399A Active JP5203387B2 (ja) | 2006-12-29 | 2007-12-28 | メチシリン耐性黄色ブドウ球菌の検出方法ならびにその検出用プライマー、プローブおよびキット |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP2118306B1 (https=) |
| JP (1) | JP5203387B2 (https=) |
| CN (1) | CN101680023B (https=) |
| CA (1) | CA2673958C (https=) |
| ES (1) | ES2403587T3 (https=) |
| WO (1) | WO2008080620A1 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2348042A1 (en) | 2001-06-04 | 2002-12-04 | Ann Huletsky | Sequences for detection and identification of methicillin-resistant staphylococcus aureus |
| US11834720B2 (en) | 2005-10-11 | 2023-12-05 | Geneohm Sciences, Inc. | Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx |
| EP2657351B1 (en) | 2007-12-21 | 2018-06-20 | Biomerieux Sa | Detection of methicillin-resistant Staphylococcus aureus |
| JP5550060B2 (ja) * | 2008-10-03 | 2014-07-16 | 独立行政法人産業技術総合研究所 | そうか病病原菌種のｐｃｒ定量用試薬キット |
| IN2014DN05831A (https=) | 2011-12-23 | 2015-06-26 | Biomerieux Sa | |
| RU2716210C2 (ru) | 2012-04-06 | 2020-03-06 | Дженеом Сайенсиз Канада, Инк. | Последовательности для обнаружения и распознавания устойчивого к метициллину золотистого стафилококка (staphylococcus aureus) (mrsa) с mrej типа xxi |
| US9080217B2 (en) * | 2012-09-21 | 2015-07-14 | Roche Molecular Systems, Inc. | Methods for detection of Clostridium difficile |
| CN108498806A (zh) * | 2018-04-13 | 2018-09-07 | 四川大学 | 一种反义肽核酸-dna四面体载体复合物及其制备方法和应用 |
| KR102264434B1 (ko) * | 2018-11-26 | 2021-06-14 | 재단법인 바이오나노헬스가드연구단 | 황색포도상구균-특이 핵산 프로브 및 이의 용도 |
| CN111549151A (zh) * | 2020-05-06 | 2020-08-18 | 南京实践医学检验有限公司 | 一种用于检测多种细菌耐药基因的试剂盒 |
| KR102714821B1 (ko) * | 2023-07-27 | 2024-10-14 | 드림디엑스 주식회사 | 항생제 내성 세균 검출용 조성물 및 이를 이용한 검출방법 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2348042A1 (en) * | 2001-06-04 | 2002-12-04 | Ann Huletsky | Sequences for detection and identification of methicillin-resistant staphylococcus aureus |
| EP1529847B1 (en) * | 2003-11-07 | 2006-04-05 | Federal Rep. of Germany repr. by the Ministry of Health & Soc. Security, the latter repr. by the Pres. of the Robert Koch Ins. | Method for detecting methicillin resistant Staphylococcus aureus (MRSA) |
| CA2606253A1 (en) * | 2005-04-21 | 2006-10-26 | Uti Limited Partnership | Pcr for mrsa sccmec typing |
| JP5027126B2 (ja) * | 2005-07-25 | 2012-09-19 | アリーア サン ディエゴ， インコーポレイテッド | リコンビナーゼポリメラーゼ増幅を多重化するための方法 |
-
2007
- 2007-12-28 CA CA2673958A patent/CA2673958C/en active Active
- 2007-12-28 WO PCT/EP2007/011458 patent/WO2008080620A1/en not_active Ceased
- 2007-12-28 JP JP2009543399A patent/JP5203387B2/ja active Active
- 2007-12-28 CN CN200780051863.XA patent/CN101680023B/zh active Active
- 2007-12-28 EP EP07857150A patent/EP2118306B1/en active Active
- 2007-12-28 ES ES07857150T patent/ES2403587T3/es active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2010514429A5 (https=) | ||
| Barken et al. | Advances in nucleic acid-based diagnostics of bacterial infections | |
| US11993804B2 (en) | Generic sample preparation | |
| US12146197B2 (en) | Multiplex diagnostic assays for Lyme disease and other tick-borne diseases | |
| JP5203387B2 (ja) | メチシリン耐性黄色ブドウ球菌の検出方法ならびにその検出用プライマー、プローブおよびキット | |
| BR112013010856B1 (pt) | método para detectar se uma sequência alvo de gardnerella vaginalis (gv) está presente em uma amostra e kit para uma reação de amplificação ou de detecção de gardnerella vaginalis | |
| JP6959257B2 (ja) | マイコプラズマ・ジェニタリウムを検出するための組成物と方法 | |
| CA2802548C (en) | Qualitative and quantitative detection of microbial nucleic acids | |
| US20140017692A1 (en) | Method and kit for detecting target nucleic acid | |
| US20160115529A1 (en) | Control nucleic acids for multiple parameters | |
| KR20120018734A (ko) | 헤파티티스 ｂ 바이러스 검출용 키트 및 이를 이용한 헤파티티스 ｂ 바이러스의 검출 방법 | |
| JP2020533974A (ja) | 呼吸器合胞体ウイルス種のニッキング及び伸長増幅反応（ｎｅａｒ） | |
| ES2269890T3 (es) | Deteccion del enterococcus spp vancomicina-resistente. | |
| JP6538660B2 (ja) | オーバーラッププライマー及び融解プローブを用いた一塩基多型の検出 | |
| CA2883066C (en) | Compositions and methods for detection of clostridium difficile | |
| WO2006102695A1 (en) | Identifying respiratory infectious organisms | |
| PT1560929E (pt) | Polinucleótidos para a amplificação e detecção de chlamydia trachomatis e neisseria gonorrhoeae | |
| JP7391321B2 (ja) | アクネ菌のｄｎａタイプの判別用オリゴヌクレオチドセット、キット及びアクネ菌のｄｎａタイプの判別方法 | |
| CA2969580C (en) | Compositions and methods for detecting mecc containing methicillin-resistant staphylococcus aureus | |
| JP7414232B2 (ja) | 皮膚常在菌の検出用オリゴヌクレオチドセット、キット及び皮膚常在菌の検出方法 | |
| US20170283888A1 (en) | Use of rnase h for the selective amplification of viral dna | |
| US20260098309A1 (en) | Compositions and methods for detecting lymphogranuloma venereum (lgv) serovars of chlamydia trachomatis | |
| JP2009533063A5 (https=) | ||
| US9267178B2 (en) | Detection and differentiation of demodex mites | |
| CA2705852C (en) | Photoinduced electron transfer (pet) primer for nucleic acid amplification |