JP2010265252A - Hair-restoring agent - Google Patents
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- JP2010265252A JP2010265252A JP2009136689A JP2009136689A JP2010265252A JP 2010265252 A JP2010265252 A JP 2010265252A JP 2009136689 A JP2009136689 A JP 2009136689A JP 2009136689 A JP2009136689 A JP 2009136689A JP 2010265252 A JP2010265252 A JP 2010265252A
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本発明は、ツバキ科ツバキ属に属するツバキ（Ｃａｍｅｌｌｉａ ｊａｐｏｎｉｃａ）の実及び／又は種子の脱脂粕の水性成分を有効成分として含有してなることを特徴とする発毛促進、育毛及び／又は養毛剤に関する。The present invention relates to a hair growth promoting, hair growing and / or hair nourishing agent characterized by comprising as an active ingredient an aqueous component of camellia (Camellia japonica) fruit and / or seed defatted cocoon belonging to the camellia family Camellia .
血液の成分は赤血球、白血球、血小板等の細胞性成分及び血漿の液性成分から成り立ち、その比率は４５：５５と言われている。それぞれの血液成分は、生体の恒常性の維持のために重要な役割を果たしている。赤血球は細胞内にあるヘモグロビンと酸素が結合して、全身組織への酸素の運搬を行い、白血球は外部から進入したウイルス、微生物及び異物を貪食破壊して排除するといった免疫に働き、血小板は凝集塊を形成して破損部位を覆い、出血を防ぐ役割をしている。さらに、血漿には栄養分、ホルモンの運搬や炭酸ガス、老廃物の回収といった役割がある。Blood components are composed of cellular components such as red blood cells, white blood cells, and platelets, and liquid components of plasma, and the ratio is said to be 45:55. Each blood component plays an important role in maintaining the homeostasis of the living body. Red blood cells combine with hemoglobin in the cells and oxygen to transport oxygen to the whole body tissue, and white blood cells act for immunity such as phagocytosis and elimination of viruses, microorganisms and foreign substances that have entered from the outside, and platelets aggregate A lump is formed to cover the damaged part and prevent bleeding. In addition, plasma plays a role in nutrients, transport of hormones, carbon dioxide, and waste collection.
近年、加齢、ストレス、喫煙、食生活の欧米化、運動不足、外的環境等によって血流が低下し、様々な体調不良を訴える人が増加している。血流の低下は、動脈硬化症、高血圧症、免疫力の低下、疲労、脱毛症、むくみ、肩こり、冷え症、手足のしびれ、皮膚のくまやくすみ等の様々な症状を引き起こすことが知られている。したがって、全身の血流循環を保つことは生体の組織や細胞の正常な機能や代謝を維持するためには欠かせず、健康で生活の質（ＱＯＬ）を維持するために重要であることが一般に知られている。In recent years, blood flow has decreased due to aging, stress, smoking, westernization of diet, lack of exercise, external environment, etc., and the number of people complaining of various physical condition is increasing. Reduced blood flow is known to cause various symptoms such as arteriosclerosis, hypertension, decreased immunity, fatigue, alopecia, swelling, stiff shoulders, coldness, numbness in the limbs, dullness and dullness in the skin Yes. Therefore, maintaining systemic blood circulation throughout the body is essential for maintaining normal functions and metabolism of living tissues and cells, and is important for maintaining healthy and quality of life (QOL). Generally known.
毛髪の成長においても血流が関係していることが知られている。毛は毛包の基底部にある毛球で作られる。その毛球は毛母細胞と毛乳頭から構成されており、毛母細胞は毛細血管から運ばれる酸素や栄養素を使い、分裂や分化を行いながら頭皮に向かって伸びていく。この過程で血流が低下し、酸素や髪の成長に必要な栄養素が不足すると、ヘアサイクルに異常を招き、髪の成長が抑制されたり、脱毛するなどの毛髪トラブルにつながる。そこで、前記の毛髪トラブルを予防及び／改善するためには、頭皮の血流を促進及び／又は改善する必要がある。It is known that blood flow is also involved in hair growth. Hair is made of hair bulbs at the base of the hair follicle. The hair bulb is composed of hair matrix cells and hair papilla, and the hair matrix cells use oxygen and nutrients carried from the capillaries to extend toward the scalp while undergoing division and differentiation. In this process, blood flow decreases, and oxygen and lack of nutrients necessary for hair growth cause abnormalities in the hair cycle, leading to hair problems such as hair growth being suppressed or hair loss. Therefore, in order to prevent and / or improve the above hair trouble, it is necessary to promote and / or improve the blood flow of the scalp.
血流を改善することにより、発毛促進や育毛効果を奏する活性物質を探索する試みは従来から検討され、これまでにタヒボエキス（特許文献１）、クマザサ属抽出物（特許文献２）、センブリやゲンチアナ（特許文献３）、トウガラシチンキ、ショウガチンキ等が提案されている。Attempts to search for an active substance that exhibits hair growth promotion and hair growth effects by improving blood flow have been studied in the past, and so far Tahibo extract (Patent Document 1), Kumasasa extract (Patent Document 2), assembly And Gentiana (patent document 3), chili tincture, ginger tincture and the like have been proposed.
これら成分や抽出物は、飲食品用途に利用する場合は、胃腸内で変質や分解を受けるリスクがあり、又、個人によっては効果が必ずしも明確でない場合もあり、実用面において有効性を発現し得るものは数少なかった。したがって、前記の発毛促進、育毛及び／又は養毛の作用を増強し得る実効性のある素材が求められていた。When these ingredients and extracts are used for food and drink applications, there is a risk of deterioration or degradation in the gastrointestinal tract, and depending on the individual, the effect may not always be clear, and the effectiveness is practically manifested. There were few things to get. Therefore, an effective material capable of enhancing the effects of hair growth promotion, hair growth and / or hair restoration has been demanded.
後述するツバキについては次のようなことが知られている。すなわち、ツバキは古来より観賞用園芸植物として利用されてきた歴史があり、種子から採取した油脂は燃料油、整髪料、高級食用油等に、木部は灰化して日本酒の醸造に、又、実の脱脂粕は農作物の肥料等に利用されてきた。脱脂粕にはサポニンやタンニンが含まれ、これを加工して殺虫防虫剤（特許文献４）、農園芸用線虫防除剤（特許文献５）等となす提案もある。しかしながら、ツバキの種子の脱脂粕に含まれる成分を経口的に摂取することで、血流を促進及び／又は改善し、発毛促進や育毛及び／又は養毛のために用いる例は見当たらない。The following is known about the camellia described later. That is, camellia has a history of being used as an ornamental horticultural plant since ancient times, and fats and oils collected from seeds are used as fuel oil, hair styling, high-grade edible oil, etc., and xylem is ashed to brew sake. The actual defatted rice bran has been used as a fertilizer for agricultural crops. The defatted cocoons contain saponins and tannins, and there are also proposals for processing them into insecticides (Patent Document 4), agricultural and horticultural nematodes control agents (Patent Document 5), and the like. However, there are no examples of oral intake of ingredients contained in camellia seed defatted silkworms that promote and / or improve blood flow and promote hair growth, hair growth and / or hair growth.
かかる現状に鑑み、本発明者らは、血流を促進及び／又は改善することにより、発毛促進、育毛及び／又は養毛、あるいは脱毛予防のための、安全かつ安定な新規素材を開発するとともに、これを産業上有効活用できる態様の組成物を提供することを課題とした。In view of the current situation, the present inventors develop a safe and stable new material for promoting hair growth, hair growth and / or hair restoration, or preventing hair loss by promoting and / or improving blood flow. At the same time, an object of the present invention is to provide a composition in an aspect in which this can be effectively used industrially.
前記課題を解決するために、本発明者らは、血流を促進及び／又は改善することに基づく発毛促進、育毛及び／又は養毛、脱毛予防のための素材について鋭意検討を重ねた結果、意外にもツバキが極めて有効であり、ツバキにはヒトや動物の血流を顕著に促進及び／又は改善して、とりわけ発毛を促進し、育毛及び／又は養毛に有用であり、脱毛を予防し得る成分が含まれていること、更に、これを飲食品、医薬品、医薬部外品、飼料等の分野において有効利用できることを見出し、本発明を完成するに至った。In order to solve the above-mentioned problems, the present inventors have conducted extensive studies on materials for promoting hair growth, hair growth and / or hair restoration, and preventing hair loss based on promoting and / or improving blood flow. Surprisingly, camellia is extremely effective, and the camellia significantly promotes and / or improves blood flow in humans and animals, especially promotes hair growth, and is useful for hair growth and / or hair growth. The present inventors have found that a component capable of preventing odors is contained and that this can be effectively used in the fields of foods and drinks, pharmaceuticals, quasi drugs, feeds, and the like, and have completed the present invention.
すなわち、本発明によれば、ツバキ科ツバキ属に属するツバキ（Ｃａｍｅｌｌｉａ ｊａｐｏｎｉｃａ）の実及び／又は種子の脱脂粕の水性成分を有効成分として含有してなる発毛促進、育毛及び／又は養毛剤（以下、単に育毛剤という）が提供される。この育毛剤において、前記水性成分は、ツバキ科ツバキ属に属するツバキ（Ｃａｍｅｌｌｉａ ｊａｐｏｎｉｃａ）の実及び／又は種子の脱脂物を水及び／又は低級アルコールで抽出処理して得られる抽出物であることが望ましい。That is, according to the present invention, a hair growth promoting, hair growing and / or hair nourishing agent (hereinafter referred to as an active ingredient) containing an aqueous component of camellia (Camellia japonica) fruit and / or seed defatted cocoon belonging to the genus Camellia camellia. Simply referred to as hair restorer). In this hair restorer, the aqueous component is an extract obtained by subjecting a camellia (Camellia japonica) fruit and / or seed defatted product to extraction with water and / or a lower alcohol. desirable.
前記水性成分はサポニン類を含むものが好ましく、該サポニン類はとりわけカメリアサポニン（Ｃａｍｅｌｌｉａｓａｐｏｎｉｎ）Ａ１、カメリアサポニン（Ｃａｍｅｌｌｉａｓａｐｏｎｉｎ）Ａ２、カメリアサポニン（Ｃａｍｅｌｌｉａｓａｐｏｎｉｎ）Ｂ１、カメリアサポニン（Ｃａｍｅｌｌｉａｓａｐｏｎｉｎ）Ｂ２、カメリアサポニン（Ｃａｍｅｌｌｉａｓａｐｏｎｉｎ）Ｃ１及び／又はカメリアサポニン（Ｃａｍｅｌｌｉａｓａｐｏｎｉｎ）Ｃ２であることがより好ましい。Preferably, the aqueous component contains saponins, among which Camellia saponins A1, Camellia saponins A2, Camellia saponins B1, Camellia saponins (Camellia saponins, Camellia saponins) More preferred is C1 and / or Camellia saponin C2.
又、前記の育毛剤は、経口的に摂取又は投与して用いることを特徴とするものであることが望ましい。Moreover, it is desirable that the hair-restoring agent is characterized by being orally ingested or administered.
本発明に係るツバキ（Ｃａｍｅｌｌｉａ ｊａｐｏｎｉｃａ）の種子の脱脂粕から抽出された水性成分は、品質安定性に優れ、血流を促進及び／又は改善することにより、発毛促進、育毛及び／又は養毛の効果を奏する。かかる効果は、前記水性成分を有効成分として含有してなる育毛剤を経口的に摂取又は投与することによって顕著に発現される。したがって、本発明の育毛剤は、髪の成長が抑制されたり、薄毛、抜け毛、脱毛等の毛髪トラブルを予防及び／又は改善するために有効利用することができる。The aqueous component extracted from the camellia (Camellia japonica) seed defatted cocoon according to the present invention has excellent quality stability and promotes and / or improves blood flow, thereby promoting hair growth, hair growth and / or hair restoration. The effect of. Such an effect is remarkably expressed by orally ingesting or administering a hair restorer comprising the aqueous component as an active ingredient. Therefore, the hair-restoring agent of the present invention can be effectively used to suppress and / or improve hair troubles such as hair growth, hair loss, hair loss and hair loss.
以下に本発明を詳細に説明する。まず、本発明の育毛剤は、ヒトや動物の血流を促進及び／又は改善して、脱毛を予防したり、発毛促進、育毛及び／又は養毛の機能を有するものであり、ツバキ科（Ｔｈｅａｃｅａｅ）のツバキ属（Ｃａｍｅｌｌｉａ）に属するツバキ（Ｃａｍｅｌｌｉａ ｊａｐｏｎｉｃａ）の実及び／又は種子の水性成分を有効成分として含有してなることを特徴とする。The present invention is described in detail below. First, the hair restorer of the present invention promotes and / or improves blood flow in humans and animals to prevent hair loss, and has functions of promoting hair growth, hair growth and / or hair nourishing. It is characterized by containing, as an active ingredient, an aqueous component of a camellia (Camellia japonica) fruit and / or seed belonging to the genus Camellia of (Theaceae).
ツバキ属に属する植物として、一般に、ツバキ節に属するツバキ（Ｃａｍｅｌｌｉａ ｊａｐｏｎｉｃａ）等、チャ節に属するチャ（Ｃ．ｓｉｎｅｎｓｉｓ）等、サザンカ節に属するサザンカ（Ｃ．ｓａｓａｎｑｕａ）等、カワリバツバキ節に属するグランサムツバキ（Ｃ．ｇｒａｎｔｈａｍｉａｎａ）等、ヤナギバサザンカ節に属するヤナギバサザンカ（Ｃ．ｓａｌｉｃｉｆｏｌｉａ）等、ヒメサザンカ節に属するヒメサザンカ（Ｃ．ｌｕｔｃｈｕｅｎｓｉｓ）等が知られているが、本発明ではツバキ節に属するものを用いる。この例としてヤブツバキ（Ｃ．ｊａｐｏｎｉｃａ ｖａｒ．ｊａｐｏｎｉｃａ）、ユキツバキ（Ｃ．ｊａｐｏｎｉｃａ ｓｕｂｓｐ．ｒｕｓｔｉｃａｎａ）、リンゴツバキ（Ｃ．ｊａｐｏｎｉｃａ ｖａｒ．ｍａｃｒｏｃａｒｐａ）、ホウザンツバキ（Ｃ．ｊａｐｏｎｉｃａ ｓｕｂｓｐ．ｈｏｚａｎｅｎｓｉｓ）、ホンコンツバキ（Ｃ．ｈｏｎｇｋｏｎｇｅｎｅｓｉｓ）、トウツバキ（Ｃ．ｒｅｔｉｃｕｌａｔａ）、サルウィンツバキ（Ｃ．ｓａｌｕｅｎｅｎｓｉｓ）、ピタールツバキのピタルディー種（Ｃ．ｐｉｔａｒｄｉｉ ｖａｒ．ｐｉｔａｒｄｉｉ）及びユンナン種（Ｃ．ｐｉｔａｒｄｉｉ ｖａｒ．ｙｕｎｎａｎｉｃａ）、金花茶（Ｃ．ｎｉｔｉｄｉｓｓｉｍａ）、ヤマツバキ（ヤブツバキと同種）、山茶花（ヤブツバキと同種）、ヤクシマツバキ（リンゴツバキと同種）等を挙げることができる。これらのツバキは日本列島、朝鮮半島、中国山東半島等で自生し又は栽培されているものを適宜に利用すればよい。As the plant belonging to the camellia genus, generally, the camellia camellia belonging to the camellia section, such as Camellia japonica, the tea belonging to the tea section (C. sinensis), the sasanqua belonging to the southern section, C. sasanqua, etc. (C. granthamiana) and the like, and the long-tailed salamander (C. salicifolia) and the like, the Hime Sasanka (C. lutchuensis) and the like belonging to the camellia section are known. Use. Examples of this include C. japonica var. Japonica, C. japonica subsp. Rusticana, C. japonica var. Macrocarpa, C. japonich. Hong Konggenesis, C. reticulata, C. salenensis, Pitaldi var. partardii, and C. partidi var. Yamabe camellia (same kind as Ayaba camellia), mountain tea flower (same kind as Ayers camellia), Kushimatsubaki can be mentioned (apple camellia and the like) and the like. What is necessary is just to utilize suitably these camellia which are growing naturally in the Japanese archipelago, the Korean peninsula, the Shandong peninsula of China, etc.
本発明では、前記のツバキの実及び／又は種子を圧搾処理、ヘキサンやヘプタン等の疎水性有機溶媒又は液化二酸化炭素、液化プロパン等の液化ガスを用いた超臨界抽出処理等に供して、常法により油分を抽出した残渣である脱脂物を、前記水性成分を採取するための原料とすることが望ましい。ここで、ツバキの実及び／又は種子は早熟実及び成熟実のいずれでもよく、これらの種子を用いてもよいが、脱脂物及び有効成分の収量の点から成熟実又はその種子を用いることが望ましい。より好ましくは種子を用いる。本発明では、成熟実から得られる種子を１〜２週間程度、天日等で乾燥させたものを用いるのがよい。In the present invention, the above camellia nuts and / or seeds are subjected to a compression treatment, a supercritical extraction treatment using a hydrophobic organic solvent such as hexane or heptane, or a liquefied gas such as liquefied carbon dioxide or liquefied propane. Desirably, a defatted product, which is a residue obtained by extracting oil by a method, is used as a raw material for collecting the aqueous component. Here, the camellia seeds and / or seeds may be either early-ripening seeds or mature seeds, and these seeds may be used. From the viewpoint of the yield of the defatted product and the active ingredient, mature seeds or seeds thereof may be used. desirable. More preferably seeds are used. In the present invention, it is preferable to use seeds obtained from mature fruits dried for about 1 to 2 weeks in the sun.
前記脱脂粕の水性成分は任意の方法で製造することができるが、水及び／又は低級アルコールを用いて抽出処理するのが好ましい。低級アルコールは、その炭素数が大きくなると脱脂粕中の油性物質が抽出される傾向が大きくなるため、炭素数が５程度までのものが望ましく、メタノール、エタノール、ノルマルプロパノール、イソプロパノール、ノルマルブタノール、イソブタノール等を例示できる。炭素数が大きい低級アルコールを使用する場合は、脱脂粕中の油性成分の抽出を抑制するために含水率を高めるのがよい。例えば、プロパノールの場合の含水率は約２０質量％〜約５０質量％とし、ブタノールの場合の含水率は約４０質量％〜約７０質量％とする。望ましい抽出溶媒は水、メタノール及びエタノール、及び、これらの含水アルコール（含水率：０〜１００質量％）である。The aqueous component of the defatted lees can be produced by any method, but it is preferable to extract it with water and / or lower alcohol. The lower alcohol has a tendency to extract the oily substance in the defatted soot as the carbon number of the lower alcohol increases. Therefore, those having a carbon number of up to about 5 are desirable, such as methanol, ethanol, normal propanol, isopropanol, normal butanol, isopropanol. Examples include butanol. When using a lower alcohol having a large carbon number, it is preferable to increase the water content in order to suppress the extraction of oily components in the defatted soot. For example, the water content in the case of propanol is about 20% by mass to about 50% by mass, and the water content in the case of butanol is about 40% by mass to about 70% by mass. Desirable extraction solvents are water, methanol and ethanol, and water-containing alcohols thereof (water content: 0 to 100% by mass).
脱脂粕を抽出するには、脱脂粕１質量部に対して前記抽出溶媒を約１質量倍〜約３０質量倍加え、常圧下又は１〜５気圧の加圧下、常温ないしは約１２０℃で、約１０分〜約３時間、必要に応じて撹拌して混合後、常温に冷却して濾過し、濾液を減圧乾燥、噴霧乾燥、凍結乾燥等の適当な手段により濃縮、乾燥する。尚、乾燥物は適宜に粉砕処理してもよい。このようにして本発明に係る脱脂粕の水性成分である淡黄色ないし黄赤色の固体を得ることができる。前記抽出方法は、一旦抽出処理した抽出残渣を繰り返し抽出処理したり、１〜３気圧の加圧下、約１００℃〜約１３０℃で行うことが望ましい。これにより本発明に係る水性成分の収量が増える。この水性成分はサポニン、タンニン等を含む。In order to extract the defatted soot, the extraction solvent is added in an amount of about 1 to about 30 times by mass with respect to 1 part by weight of the defatted soot, and at about normal temperature or about 1 to 5 atm. After stirring and mixing for 10 minutes to about 3 hours as necessary, the mixture is cooled to room temperature and filtered, and the filtrate is concentrated and dried by an appropriate means such as drying under reduced pressure, spray drying or freeze drying. The dried product may be appropriately pulverized. In this way, a pale yellow to yellow-red solid that is an aqueous component of the defatted soot according to the present invention can be obtained. The extraction method is preferably performed by repeatedly extracting the extraction residue once extracted, or under a pressure of 1 to 3 atmospheres at about 100 ° C. to about 130 ° C. This increases the yield of the aqueous component according to the present invention. This aqueous component includes saponins, tannins and the like.
水性成分に含まれるサポニンとして、３β−［２−Ｏ−β−Ｄ−ガラクトピラノシル−３−Ｏ−（２−Ｏ−β−Ｄ−グルコピラノシル−α−Ｌ−アラビノピラノシル）−β−Ｄ−グルコピラヌロノシルオキシ］オレアナ−１２−エン−１６α，２２α，２８−トリオール２２−［（Ｚ）−２−メチル−２−プテノアート］であるカメリアサポニン（Ｃａｍｅｌｌｉａｓａｐｏｎｉｎ）Ａ１、３β−［２−Ｏ−β−Ｄ−ガラクトピラノシル−３−Ｏ−（２−Ｏ−β−Ｄ−グルコピラノシル−α−Ｌ−アラビノピラノシル）−β−Ｄ−グルコピラヌロノシルオキシ）オレアナ−１２−エン−１６α，２２α，２８−トリオール２２−［（Ｅ）−２−メチル−２−ブテノアート］であるカメリアサポニン（Ｃａｍｅｌｌｉａｓａｐｏｎｉｎ）Ａ２、３β−［２−Ｏ−β−Ｄ−ガラクトピラノシル−３−Ｏ−（２−Ｏ−β−Ｄ−グルコピラノシル−α−Ｌ−アラビノピラノシル）−β−Ｄ−グルコピラヌロノシルオキシ）−１６α，２８−ジヒドロキシ−２２α−［［（Ｚ）−２−メチル−２−ブテノイル］オキシ］オレアナ−１２−エン−２３−アールであるカメリアサポニン（Ｃａｍｅｌｌｉａｓａｐｏｎｉｎ）Ｂ１、３β−［［２−Ｏ−β−Ｄ−ガラクトピラノシル−３−Ｏ−（２−Ｏ−β−Ｄ−グルコピラノシル−α−Ｌ−アラビノピラノシル）−β−Ｄ−グルコピラヌロノシル）オキシ］−１６α，２８−ジヒドロキシ−２２α−［［（Ｅ）−２−メチル−２−ブテノイル］オキシ］オレアナ−１２−エン−２３−アールであるカメリアサポニン（Ｃａｍｅｌｌｉａｓａｐｏｎｉｎ）Ｂ２、３β−［２−Ｏ−β−Ｄ−ガラクトピラノシル−３−Ｏ−（２−Ｏ−β−Ｄ−グルコピラノシル−α−Ｌ−アラビノピラノシル）−β−Ｄ−グルコピラヌロノシルオキシ］オレアナ−１２−エン−１６α，２２α，２３，２８−テトラオール２２−［（Ｚ）−２−メチル−２−ブテノアート］であるカメリアサポニン（Ｃａｍｅｌｌｉａｓａｐｏｎｉｎ）Ｃ１、及び、３β−［２−Ｏ−β−Ｄ−ガラクトピラノシル−３−Ｏ−（２−Ｏ−β−Ｄ−グルコピラノシル−α−Ｌ−アラビノピラノシル）−β−Ｄ−グルコピラヌロノシルオキシ］オレアナ−１２−エン−１６α，２２α，２３，２８−テトラオール２２−［（Ｅ）−２−メチル−２−ブテノアート］であるカメリアサポニン（Ｃａｍｅｌｌｉａｓａｐｏｎｉｎ）Ｃ２等を例示することができ、これらのサポニンはツバキに特異的に含まれている。As a saponin contained in the aqueous component, 3β- [2-O-β-D-galactopyranosyl-3-O- (2-O-β-D-glucopyranosyl-α-L-arabinopyranosyl)- Camellia saponin A1, 3β-, which is [β-D-glucopyranuronosyloxy] oleana-12-ene-16α, 22α, 28-triol 22-[(Z) -2-methyl-2-ptenoate] [2-O-β-D-galactopyranosyl-3-O- (2-O-β-D-glucopyranosyl-α-L-arabinopyranosyl) -β-D-glucopyranuronosyloxy ) Oleana-12-ene-16α, 22α, 28-triol 22-[(E) -2-methyl-2-butenoate] Camellia saponin A2, 3β- [2 O-β-D-galactopyranosyl-3-O- (2-O-β-D-glucopyranosyl-α-L-arabinopyranosyl) -β-D-glucopyranuronosyloxy) -16α , 28-dihydroxy-22α-[[(Z) -2-methyl-2-butenoyl] oxy] oleana-12-en-23-al Camellia saponin B1, 3β-[[2-O-β -D-galactopyranosyl-3-O- (2-O-β-D-glucopyranosyl-α-L-arabinopyranosyl) -β-D-glucopyranuronosyl) oxy] -16α, 28 Camellia saponin B2, 3β- [2 which is -dihydroxy-22α-[[((E) -2-methyl-2-butenoyl] oxy] oleana-12-en-23-al O-β-D-galactopyranosyl-3-O- (2-O-β-D-glucopyranosyl-α-L-arabinopyranosyl) -β-D-glucopyranuronosyloxy] oleana Camellia saponin C1, which is 12-ene-16α, 22α, 23,28-tetraol 22-[(Z) -2-methyl-2-butenoate], and 3β- [2-O-β-D -Galactopyranosyl-3-O- (2-O-β-D-glucopyranosyl-α-L-arabinopyranosyl) -β-D-glucopyranuronosyloxy] oleana-12-ene-16α , 22α, 23,28-tetraol 22-[(E) -2-methyl-2-butenoate], such as Camellia saponin C2, and the like. Ponin is specifically contained in camellia.
本発明の育毛剤は、その有効成分としての前記水性成分を固体状、ペースト状又は液体状の形態となし、これをそのまま育毛剤としてよいが、必要に応じて本発明の育毛剤が利用される用途における公知の添加物を併用して、常法により含有せしめて組成物として調製することもできる。ここで、公知の添加物は経口摂取するために通常利用されるものが望ましく、例えば、賦形剤、結合剤、崩壊剤、滑沢剤、湿潤剤、流動化剤、保存剤、界面活性剤、安定剤、希釈剤、溶解剤、等張化剤、殺菌剤、防腐剤、矯味剤、矯臭剤、着色剤、香料等の添加物質を使用でき、又、育毛効果を有する既知成分やその含有素材を併用してもよい。In the hair restorer of the present invention, the aqueous component as an active ingredient is in the form of a solid, paste or liquid, and this may be used as it is, but the hair restorer of the present invention is used as necessary. It is also possible to prepare a composition by using a conventional method in combination with known additives for the purpose of use. Here, known additives are preferably those commonly used for ingestion, for example, excipients, binders, disintegrants, lubricants, wetting agents, fluidizing agents, preservatives, surfactants. , Stabilizers, diluents, solubilizers, tonicity agents, bactericides, preservatives, flavoring agents, flavoring agents, coloring agents, fragrances, etc. can be used, and known ingredients with hair-growth effects and their contents You may use a material together.
育毛作用が既知の成分や素材としては、前記のもののほか朝鮮人参エキス、ニンニクエキス、カラギーナン、ブラックジンジャー抽出物、スギナ抽出物、ノコギリヤシ果実抽出物、カボチャ種子エキス、緑茶葉エキス、エンメイソウ、オタネニンジン、オトギリソウ、サルビア、ボダイジュ、ラカンカ、ヒバマタ、ミノキシジル、パントテン酸類、ビタミンＥ、パントテン酸、ビオチン等を例示できる。尚、本発明はこれらの例示によって何ら限定されるものではない。In addition to the above-mentioned ingredients and materials with known hair-growth action, ginseng extract, garlic extract, carrageenan, black ginger extract, horse chestnut extract, saw palmetto fruit extract, pumpkin seed extract, green tea leaf extract, emerythra, ginseng, Examples include hypericum, salvia, bodyfish, lacanka, hibermata, minoxidil, pantothenic acids, vitamin E, pantothenic acid, biotin and the like. In addition, this invention is not limited at all by these illustrations.
本発明においては、前述した育毛剤をそのままの形態で飲食品、医薬品、医薬部外品、飼料、その他産業分野の様々な製品として利用することができ、あるいは該各種製品の配合原料の一部として使用する態様でも利用できる。とりわけ、発毛促進、育毛及び／又は養毛のための経口組成物となすことが好ましく、この経口組成物の最も好適な態様は飲食品である。この例を以下に述べるが、本発明はこれにより限定されるものではない。In the present invention, the above-described hair restorer can be used as it is in various forms of foods and drinks, pharmaceuticals, quasi-drugs, feeds, and other industrial fields, or a part of the ingredients of the various products. It can utilize also in the aspect used as. In particular, it is preferable to form an oral composition for promoting hair growth, hair growth and / or hair restoration, and the most preferred embodiment of this oral composition is a food or drink. This example will be described below, but the present invention is not limited thereby.
本発明の育毛剤を経口組成物とする場合の形態は、顆粒剤、錠剤、カプセル剤、液剤等の経口用製剤となすことが可能である。かかる製剤組成物における前記水性成分の含有量は、併用原料の種類や含有量等により一律に規定し難いが、概ね０．０１質量％〜９０質量％程度、より望ましくは約０．１質量％〜約７０質量％である。前記含有量が約０．０１質量％を下回ると本発明の所望効果が認められなくなり、約９０質量％を超えると実用的な製剤組成物を調製することが難しくなる。本発明の育毛剤は、これを望ましくは経口的に摂取又は投与する態様で利用する。経口摂取又は投与する場合の本発明の育毛剤の好適な量の目安は、該剤に含まれる前記水性成分ベースで、ヒト成人１日あたり約１０ｍｇ〜約１，０００ｍｇ、望ましくは約３０ｍｇ〜約５００ｍｇ、更に望ましくは約５０ｍｇ〜約３００ｍｇである。When the hair restorer of the present invention is used as an oral composition, it can be made into oral preparations such as granules, tablets, capsules and liquids. The content of the aqueous component in such a pharmaceutical composition is difficult to define uniformly depending on the type and content of the combined raw materials, but is generally about 0.01% to 90% by mass, more preferably about 0.1% by mass. To about 70% by weight. When the content is less than about 0.01% by mass, the desired effect of the present invention is not recognized. When the content exceeds about 90% by mass, it is difficult to prepare a practical pharmaceutical composition. The hair-restoring agent of the present invention is preferably used in such a manner that it is taken or administered orally. A suitable amount of the hair restorer of the present invention when ingested or administered is about 10 mg to about 1,000 mg, preferably about 30 mg to about 1,000 mg per day for a human adult, based on the aqueous component contained in the agent. 500 mg, more desirably about 50 mg to about 300 mg.
本発明の育毛剤は、これ自体を飲食品、医薬品、飼料その他産業分野の様々な形態の製品とすることができ、あるいは、かかる製品の配合原料の一部とする態様でも利用することができる。実用的な用途としては飲食品がよい。The hair restorer of the present invention can be used as a product in various forms of food and drink, pharmaceuticals, feed and other industrial fields, or can be used as a part of a blended raw material of such a product. . For practical applications, food and drink are good.
飲食品の具体例として、野菜ジュース、果汁飲料、清涼飲料、茶等の飲料類、即席麺、スープ、ゼリー、プリン、ヨーグルト、ケーキプレミックス製品、菓子類、ふりかけ、味噌、醤油、ソース、ドレッシング、マヨネーズ、植物性クリーム、焼肉用たれや麺つゆ等の調味料、麺類、うどん、蕎麦、スパゲッティ、ハムやソーセージ等の畜肉魚肉加工食品、ハンバーグ、コロッケ、ふりかけ、佃煮、ジャム、牛乳、クリーム、バター、スプレッドやチーズ等の粉末状、固形状又は液状の乳製品、マーガリン、パン、ケーキ、クッキー、チョコレート、キャンディー、グミ、ガム等の各種一般加工食品のほか、粉末状、顆粒状、丸剤状、錠剤状、ソフトカプセル状、ハードカプセル状、ペースト状又は液体状の栄養補助食品、特定保健用食品、機能性食品、健康食品、濃厚流動食や嚥下障害用食品の治療食等を挙げることができる。Specific examples of food and drink include beverages such as vegetable juice, fruit juice drinks, soft drinks, tea, instant noodles, soup, jelly, pudding, yogurt, cake premix products, confectionery, sprinkles, miso, soy sauce, sauce, dressing , Mayonnaise, vegetable cream, seasonings such as grilled meat sauce and noodle soup, noodles, udon, soba noodles, spaghetti, processed meat and fish products such as ham and sausage, hamburger, croquette, sprinkle, boiled, jam, milk, cream, Various processed foods such as butter, spread and cheese, powdered, solid or liquid dairy products, margarine, bread, cake, cookies, chocolate, candy, gummi, gum, etc., powder, granules, pills , Tablet, soft capsule, hard capsule, paste or liquid dietary supplement, food for specified health use, Potential food, mention may be made of health food products, the concentrated liquid diet and dysphagia for food diet and the like.
尚、本発明の飲食品においては、これがツバキ科（Ｔｈｅａｃｅａｅ）のツバキ属（Ｃａｍｅｌｌｉａ）に属するツバキ（Ｃａｍｅｌｌｉａ ｊａｐｏｎｉｃａ）の実及び／又は種子の水性成分を有効成分として含有してなる旨、発毛促進、育毛及び／又は養毛するためのものである旨のうち少なくとも１つの表示を付した態様とすることができる。In addition, in the food / beverage products of this invention, the hair growth that this contains the water component of the camellia (Camellia japonica) and / or seed which belong to the camellia genus (Camelia) of the Camellia family (Theaceae) as an active ingredient, It can be set as the aspect which attached | subjected at least 1 indication among the things for promoting, hair-growth, and / or hair-growth.
これらの飲食品を製造するには、本発明の育毛剤と公知の原材料を用い、あるいは公知の原材料の一部を前記の育毛剤で置き換え、常法によって製造すればよい。例えば、本発明の育毛剤を、必要に応じてグルコース（ブドウ糖）、デキストリン、乳糖、澱粉又はその加工物、セルロース粉末等の賦形剤、ビタミン、ミネラル、動植物や魚介類の油脂、たん白（動植物や酵母由来の蛋白質、その加水分解物等を含む）、糖質、色素、香料、酸化防止剤、界面活性剤、その他の食用添加物、各種栄養機能成分を含む粉末やエキス類等の食用素材とともに混合して粉末、顆粒、ペレット、錠剤等の形状に加工したり、常法により前記例の一般加工食品に加工処理したり、これらを混合した液状物をゼラチン、アルギン酸ナトリウム、カルボキシメチルセルロース等の被覆剤で被覆してカプセルに成形したり、飲料（ドリンク類）の形態に加工して、栄養補助食品や健康食品として利用することは好適である。とりわけ錠剤、カプセル剤やドリンク剤が望ましい。In order to produce these foods and drinks, the hair restorer of the present invention and known raw materials may be used, or a part of the known raw materials may be replaced with the above-mentioned hair restorer and produced by conventional methods. For example, the hair restorer of the present invention may be added to excipients such as glucose (dextrose), dextrin, lactose, starch or processed product thereof, cellulose powder, vitamins, minerals, fats and oils of animals and plants and seafood, protein (if necessary) Edibles such as powders and extracts containing various nutritional functional ingredients, including proteins derived from animals and plants and yeast, and hydrolysates thereof, carbohydrates, pigments, fragrances, antioxidants, surfactants, other edible additives Mixed with raw materials and processed into powders, granules, pellets, tablets, etc., processed into general processed foods of the above examples by conventional methods, mixed liquids such as gelatin, sodium alginate, carboxymethyl cellulose, etc. It is preferable to use it as a dietary supplement or health food by coating it with the above-mentioned coating agent and forming it into a capsule, or processing it into a beverage (drinks) form. In particular, tablets, capsules and drinks are desirable.
かかる飲食品に配合する本発明の育毛剤の比率は、飲食品の形態、本発明の育毛剤中の前記水性成分（ツバキ科ツバキ属ツバキの実及び／又は種子の水性成分）の含量、他の配合原料の種類や成分や配合量等のちがいにより一律に規定しがたいが、飲食品中の前記水性成分の含量が約０．０１質量％〜約９０質量％、より望ましくは約１質量％〜約５０質量％となるように、本発明の育毛剤をその他の飲食品製造用公知原料と適宜に組み合わせて処方を設計し、常法に従い目的とする飲食品を調製すればよい。前記水性成分の含量が約０．０１質量％を下回るような飲食品では前記水性成分による所望効果を期待するために多量の当該飲食品を摂取しなければならず、一方、前記水性成分の量が約９０質量％を超えると実用的な飲食品を製造することが困難になることがある。本発明の飲食品は、ヒト成人の場合１日あたりの前記水性成分の摂取量の目安を約１０ｍｇ〜約１，０００ｍｇ、望ましくは約３０ｍｇ〜約５００ｍｇ、更に望ましくは約５０ｍｇ〜約３００ｍｇとして任意の方法、例えば、食事の摂取と同時又は前後に、経口摂取、経管投与等の方法で体内に取り込むことができる。The ratio of the hair restorer of the present invention to be blended in such food and drink is the form of the food and drink, the content of the aqueous component (the camellia camellia camellia and / or seed aqueous component) in the hair restorer of the present invention, etc. Although it is difficult to specify uniformly depending on the type, component, and blending amount of the raw material, the content of the aqueous component in the food and drink is about 0.01% by weight to about 90% by weight, more preferably about 1% by weight. What is necessary is just to prepare the target food-drinks according to a conventional method, designing a prescription by combining suitably the hair restorer of this invention with the other well-known raw material for food-drinks manufacture so that it may become%-about 50 mass%. In foods and beverages in which the content of the aqueous component is less than about 0.01% by mass, a large amount of the food and beverages must be ingested in order to expect the desired effect of the aqueous component, while the amount of the aqueous component If it exceeds about 90% by mass, it may be difficult to produce a practical food or drink. In the case of a human adult, the food / beverage product of the present invention can be arbitrarily selected so that the daily intake of the aqueous component is about 10 mg to about 1,000 mg, desirably about 30 mg to about 500 mg, more desirably about 50 mg to about 300 mg. This method can be taken into the body at the same time as, for example, or before or after meal intake, by oral intake, tube administration, or the like.
本発明の育毛剤を用いる医薬品は、前記の育毛剤に本発明の趣旨に反しない公知の賦形剤や添加物を適宜に加え、常法により加工して錠剤、カプセル剤、顆粒剤、散剤、液剤等の製剤となすことができる。経口あるいは経腸投与して、薄毛、抜け毛、円形脱毛症、男性型脱毛症（ＡＧＡ）、びまん性脱毛症、分娩後脱毛症等の予防又は治療のために適用する。本発明の育毛剤の配合量はその形態や前記医薬製剤の種類、形態、用法及び用量等により一律に設定し難いが、前記水性成分の含量として概ね０．０１質量％〜５０質量％である。経口投与する場合の摂取量はとくに限定されるものではないが、例えば、前記水性成分をベースにして、ヒト成人１日あたり約０．１ｍｇ〜約１，０００ｍｇ、望ましくは約１ｍｇ〜約５００ｍｇ、更に望ましくは約１０ｍｇ〜約３００ｍｇである。The pharmaceutical agent using the hair restorer of the present invention is prepared by appropriately adding known excipients and additives not contrary to the gist of the present invention to the above-mentioned hair restorer, and processing by a conventional method to produce tablets, capsules, granules, powders It can be made into preparations such as liquids. Oral or enteral administration is applied to prevent or treat thinning hair, hair loss, alopecia areata, androgenetic alopecia (AGA), diffuse alopecia, postpartum alopecia and the like. The blending amount of the hair restorer of the present invention is difficult to set uniformly depending on its form and the type, form, usage, dosage and the like of the pharmaceutical preparation, but is generally 0.01% by mass to 50% by mass as the content of the aqueous component. . The amount of intake when orally administered is not particularly limited. For example, based on the aqueous component, about 0.1 mg to about 1,000 mg, preferably about 1 mg to about 500 mg per day for a human adult, More desirably, it is about 10 mg to about 300 mg.
又、本発明の育毛剤をペットフードや家畜用飼料に適用するには、前記飲食品の場合と同様に、公知の各種飼料や飲用水に配合したり、公知の原材料、添加物とともに錠剤状、顆粒状、カプセル状等の製剤形態のものに加工することができる。これらの場合、本発明の育毛剤の配合量は、前記水性成分の含量として約０．０１質量％〜約９０質量％、より望ましくは約１質量％〜約５０質量％であり、１日あたりの摂取量の目安は、前記水性成分を基準として、適用動物の体重（ｋｇ）あたり約０．１ｍｇ〜約１００ｍｇ、より望ましくは約０．５ｍｇ〜約５０ｍｇである。In addition, in order to apply the hair restorer of the present invention to pet food and livestock feed, it is blended into various known feeds and drinking water as in the case of the foods and drinks, or is tableted with known raw materials and additives. , Granules and capsules can be processed. In these cases, the amount of the hair restorer of the present invention is about 0.01% by weight to about 90% by weight, more preferably about 1% by weight to about 50% by weight as the content of the aqueous component. The standard of the intake amount is about 0.1 mg to about 100 mg, more desirably about 0.5 mg to about 50 mg per body weight (kg) of the applied animal, based on the aqueous component.
製造例１
長崎県五島産ヤブツバキ（Ｃ．ｊａｐｏｎｉｃａ ｖａｒ．ｊａｐｏｎｉｃａ）の乾燥種子を粗粉砕して蒸煮後、圧搾して圧搾油を分離した圧搾粕を得、次いで圧搾粕にノルマルヘキサンを加えて常法により抽出処理し、抽出液を分離して抽出粕を採取した。この抽出粕をノルマルヘキサンで洗浄して油分を取り除き脱脂物を採取した。この脱脂物１００ｇに水３００ｍＬを加え、常圧下、８５℃に加熱して１時間適宜に撹拌した後、室温まで冷却し、濾過して濾液を分離した。この濾過残渣に再度水２００ｍＬを加えて同様に加熱、攪拌、冷却後、濾過して濾液を採取した。両濾液を合わせて減圧下に濃縮し、凍結乾燥及び粉砕して、本発明に係る水性成分を含む粉末（試料１とする）１６．４ｇを得た。この粉末は、これを加水分解してＨＰＬＣ分析したところ、サポニンのアグリコンであるサポゲニンを１６．８％、フラボノールの一種であるケンフェロールを２．１％含むものであった。Production Example 1
After coarsely crushing and drying dried seeds of C. japonica var. Japonica from Goto, Nagasaki Prefecture, press squeezed to separate the compressed oil is obtained. After processing, the extract was separated and the extract was collected. The extract was washed with normal hexane to remove the oil, and a defatted material was collected. Water (300 mL) was added to 100 g of the defatted material, heated to 85 ° C. under normal pressure and stirred appropriately for 1 hour, cooled to room temperature, and filtered to separate the filtrate. 200 mL of water was again added to the filtration residue, and the mixture was heated, stirred and cooled in the same manner, and then filtered to collect a filtrate. Both filtrates were combined, concentrated under reduced pressure, freeze-dried and pulverized to obtain 16.4 g of a powder containing the aqueous component according to the present invention (referred to as sample 1). When this powder was hydrolyzed and analyzed by HPLC, it contained 16.8% sapogenin, which is an aglycon of saponin, and 2.1% kaempferol, which is a kind of flavonol.
製造例２
屋久島産ヤクシマツバキ（Ｃ．ｊａｐｏｎｉｃａ ｖａｒ．ｍａｃｒｏｃａｒｐａ）の乾燥種子を製造例１に記載の方法で脱脂して脱脂物を採取した。この脱脂物１００ｇに水３００ｍＬを加え、２気圧の加圧下、１２０℃で２５分間加熱した後、室温まで冷却し、濾過して濾液を分離した。この濾過残渣に再度水２００ｍＬを加えて同様に加熱、攪拌、冷却後、濾過して濾液を採取した。両濾液を合わせて減圧下に濃縮し、凍結乾燥及び粉砕して、本発明に係る水性成分を含む粉末（試料２とする）１６．９ｇを得た。該粉末を製造例１と同様に加水分解してＨＰＬＣ分析した結果、サポゲニン含量は１５．１％であり、ケンフェロール含量は２．５％であった。Production Example 2
The dried seeds of Yakushima-made Yakushima pine (C. japonica var. Macrocarpa) were defatted by the method described in Production Example 1 and the defatted material was collected. 300 mL of water was added to 100 g of this defatted material, heated at 120 ° C. under a pressure of 2 atm for 25 minutes, cooled to room temperature, and filtered to separate the filtrate. 200 mL of water was again added to the filtration residue, and the mixture was heated, stirred and cooled in the same manner, and then filtered to collect a filtrate. Both filtrates were combined, concentrated under reduced pressure, lyophilized and pulverized to obtain 16.9 g of a powder containing the aqueous component according to the present invention (referred to as sample 2). The powder was hydrolyzed in the same manner as in Production Example 1 and analyzed by HPLC. As a result, the sapogenin content was 15.1% and the kaempferol content was 2.5%.
製造例３
製造例１に記載の方法で得た脱脂物１００ｇに含水エタノール（含水率５０％）２５０ｍＬを加え、８０℃で１時間加熱還流した後、室温まで冷却し、濾過して濾液を分離した。この濾過残渣に再度含水エタノール（含水率５０％）２００ｍＬを加えて同様に加熱し、冷却後、濾過して濾液を採取した。両濾液を合わせて減圧下に濃縮し、凍結乾燥及び粉砕して、本発明に係る水性成分を含む粉末（試料３とする）１２．３ｇを得た。該粉末を製造例１と同様に加水分解してＨＰＬＣ分析した結果、サポゲニン含量は１２．５％であり、ケンフェロール含量は２．７％であった。Production Example 3
250 g of water-containing ethanol (water content 50%) was added to 100 g of the defatted product obtained by the method described in Production Example 1, and the mixture was heated to reflux at 80 ° C. for 1 hour, cooled to room temperature, and filtered to separate the filtrate. To this filtration residue, 200 mL of hydrous ethanol (water content 50%) was added again and heated in the same manner. After cooling, the filtrate was collected by filtration. Both filtrates were combined, concentrated under reduced pressure, freeze-dried and pulverized to obtain 12.3 g of a powder containing the aqueous component according to the present invention (referred to as sample 3). The powder was hydrolyzed in the same manner as in Production Example 1 and analyzed by HPLC. As a result, the sapogenin content was 12.5% and the kaempferol content was 2.7%.
製造例４
製造例２に記載の方法で得た脱脂物１００ｇにエタノール（純度９９．５％）２００ｍＬを加え、８０℃で１時間加熱還流した後、室温まで冷却し、濾過して濾液を分離した。この濾過残渣に再度エタノール（純度９９．５％）２００ｍＬを加えて同様に加熱し、冷却後、濾過して濾液を採取した。両濾液を合わせて減圧下に濃縮し、凍結乾燥及び粉砕して、本発明に係る水性成分を含む粉末（試料４とする）４．３ｇを得た。該粉末を製造例１と同様に加水分解してＨＰＬＣ分析した結果、サポゲニン含量は１４．０％であり、ケンフェロール含量は２．５％であった。Production Example 4
Ethanol (purity 99.5%) 200 mL was added to 100 g of the defatted product obtained by the method described in Production Example 2, and the mixture was heated to reflux at 80 ° C. for 1 hour, cooled to room temperature, and filtered to separate the filtrate. 200 mL of ethanol (purity 99.5%) was again added to the filtration residue and heated in the same manner. After cooling, the filtrate was collected by filtration. Both filtrates were combined, concentrated under reduced pressure, lyophilized and pulverized to obtain 4.3 g of a powder (referred to as sample 4) containing an aqueous component according to the present invention. The powder was hydrolyzed in the same manner as in Production Example 1 and analyzed by HPLC. As a result, the sapogenin content was 14.0% and the kaempferol content was 2.5%.
製造例５
製造例１において、乾燥種子を未熟実（種子を含む実全体）におきかえること以外は同様に処理して、脱脂粕を得た後、これから水性成分を含む粉末（試料５とする）１２．８ｇを得た。該粉末を製造例１と同様に加水分解してＨＰＬＣ分析した結果、サポゲニン含量は１３．２％であり、ケンフェロール含量は２．４％であった。Production Example 5
In Production Example 1, except that the dried seed was replaced with immature fruit (whole seed-containing seed), the same treatment was performed to obtain a defatted koji, and then 12.8 g of a powder containing an aqueous component (referred to as sample 5). Got. The powder was hydrolyzed in the same manner as in Production Example 1 and analyzed by HPLC. As a result, the sapogenin content was 13.2% and the kaempferol content was 2.4%.
製造例６
ツバキ科に属する植物であるチャ（Ｃａｍｅｌｌｉａ ｓｉｎｅｎｓｉｓ）の乾燥葉１００ｇに水３００ｍＬを加えて、常圧下、８５℃に加熱して２０分間適宜に撹拌した後、室温まで冷却し、濾過して濾液を分離した。この濾過残渣に再度水２００ｍＬを加えて同様に加熱し、攪拌、冷却後、濾過して濾液を採取した。両濾液を合わせて減圧下に濃縮した。その濃縮溶液を、合成吸着剤のダイヤイオン ＨＰ−２０（三菱化学（株）製）を充填したカラムクロマトグラフィーに供した。水５００ｍＬを通液して洗浄した後、含水エタノール（含水率３０％）５００ｍＬを通液し、該含水エタノールに可溶の茶カテキン含有溶液を回収した。この溶液を減圧下に濃縮し、凍結乾燥及び粉砕して、粉末（比較試料１とする）２．５ｇを得た。該粉末をＨＰＬＣ分析した結果、カテキン類の含量は９１．８％であった。Production Example 6
Water (300 mL) is added to 100 g of dried leaves of Camellia sinensis, a plant belonging to the camellia family, heated to 85 ° C. under normal pressure, stirred for 20 minutes, cooled to room temperature, filtered, and the filtrate was filtered. separated. To this filtration residue, 200 mL of water was added again and heated in the same manner. After stirring and cooling, the filtrate was collected by filtration. Both filtrates were combined and concentrated under reduced pressure. The concentrated solution was subjected to column chromatography packed with a synthetic adsorbent Diaion HP-20 (manufactured by Mitsubishi Chemical Corporation). After washing by passing 500 mL of water, 500 mL of water-containing ethanol (water content 30%) was passed through to collect a tea catechin-containing solution soluble in the water-containing ethanol. This solution was concentrated under reduced pressure, freeze-dried and pulverized to obtain 2.5 g of powder (referred to as Comparative Sample 1). As a result of HPLC analysis of the powder, the content of catechins was 91.8%.
試験例１：血流量の増大作用
試験に参加することに同意が得られた４４〜６５歳までの薄毛の男性３６名を被験者として、１群６名に群分けして二重盲検法により試験を行った。まず、被験者を温度２４±２℃、湿度５０±１０％に制御した恒温・恒湿の部屋に入室させ、１０分間安静に待機させた。１０分後に、試験試料を摂取する前の手の甲及び頭皮の血流量をレーザードップラー（Ｐｅｒｉｍｅｄ社製、ＰｅｒｉＳｃａｎ ＰＩＭＩＩ）を用いて測定した。次に、対照群（プラセボ群）には、被験者が色で判別できないように着色したハードカプセル（ゼラチン製。以下同様）にデキストリンを充填したものを、その他の群には、前記同様に着色したハードカプセルに各試料を充填したものをそれぞれ水１００ｍＬとともに摂取してもらい、３０分後、６０分後に再び前記と同様に手の甲及び頭皮の血流量を測定した。Test Example 1: 36 males with 44 to 65 years old who had consented to participate in the blood flow increasing effect test were divided into 6 groups per group by double blind method. A test was conducted. First, the subject entered a room of constant temperature and humidity controlled at a temperature of 24 ± 2 ° C. and a humidity of 50 ± 10%, and was allowed to stand quietly for 10 minutes. Ten minutes later, the blood flow in the back of the hand and scalp before taking the test sample was measured using a laser Doppler (Periscan, PeriScan PIMII). Next, in the control group (placebo group), hard capsules (made of gelatin; the same applies hereinafter) filled with dextrin so that the subject cannot distinguish by color, and in the other groups, hard capsules colored as described above. Each sample was ingested with 100 mL of water, and after 30 minutes and 60 minutes, blood flow in the back of the hand and scalp was measured again in the same manner as described above.
この結果を表１及び表２に示す。同表において、数値はプラセボ及び被験物質を摂取する前の値を１００としたときの相対値として、平均値±標準偏差で表わした（ｎ＝６、ＡＮＯＶＡ解析）。表１及び表２のデータから、プラセボを摂取した被験者の手の甲及び頭皮の血流には有意な変化はみられず、比較試料１を摂取した群は、摂取３０分後は血流量に有意な差は認められなかったが、摂取６０分後においてはプラセボ群と比べて手の甲及び頭皮の血流量の値が有意に高かった。又、試料１〜５を摂取した群においては、プラセボを摂取した群よりも、３０分後、６０分後の両時間ともに、手の甲及び頭皮の血流量の値が有意に高かった。これにより、本発明に係る試料を摂取することにより、血流量が増加することが明らかとなった。又、その効果は比較試料１の場合よりも強かった。The results are shown in Tables 1 and 2. In the table, the numerical values are expressed as average values ± standard deviation (n = 6, ANOVA analysis) as relative values when the value before taking the placebo and the test substance is 100. From the data in Tables 1 and 2, no significant changes were observed in the blood flow of the back of the hand and scalp of subjects who took placebo, and the group that took Comparative Sample 1 showed significant changes in blood flow after 30 minutes of ingestion. Although no difference was observed, the blood flow values of the back of the hand and scalp were significantly higher at 60 minutes after ingestion compared to the placebo group. Moreover, in the group which ingested the samples 1-5, the value of the blood flow volume of the back of the hand and the scalp was significantly higher at both 30 minutes and 60 minutes after the placebo. Thereby, it became clear by ingesting the sample which concerns on this invention that a blood flow rate increases. The effect was stronger than that of Comparative Sample 1.
試験例２：血流促進改善効果を有する成分
血流促進改善作用を有する実質的な有効成分を解明するために、製造例１において得られた試料１の粉末を次の条件で高速液体クロマトグラフィー（ＨＰＬＣ）に供して分画処理を行った。装置：（株）島津製作所製ＬＣ−１０Ａシリーズ、カラム：（株）ナカライテスク製ＣＯＳＭＯＳＩＬ５Ｃ１８−ＭＳ−ＩＩ（１０×２５０ｍｍ）、溶離液：Ａ液が０．１％酢酸、Ｂ液が０．１％酢酸及び４０％アセトニトリルであり、Ａ液とＢ液のグラジェント分析、流速：２ｍＬ／ｍｉｎ、２１０ｎｍにおけるＵＶ検出。試料１を０．１％酢酸及び４０％アセトニトリル溶液に溶解し、０．４５μｍのメンブランフィルターで濾過を行った。その溶液をＨＰＬＣに供して、サポニンが溶出されるフラクションにあわせて繰り返し分取を行い、ツバキに特異的に含まれるツバキサポニン類を精製した。又、上記のサポニンが溶出される部分以外も分取を行った。これらを減圧下に濃縮し、凍結乾燥して、ツバキサポニン類の粉末（試料６とする）及び非ツバキサポニン類の粉末（試料７とする）を得た。試料６はカメリアサポニンＡ１、Ａ２、Ｂ１、Ｂ２、Ｃ１及びＣ２を含むものであった。Test Example 2: Components having an effect of improving blood flow promotion In order to elucidate a substantially effective ingredient having an action of improving blood flow, the powder of Sample 1 obtained in Production Example 1 was subjected to high performance liquid chromatography under the following conditions. (HPLC) was subjected to fractionation treatment. Apparatus: LC-10A series manufactured by Shimadzu Corporation, column: COSMOSIL5C18-MS-II (10 × 250 mm) manufactured by Nacalai Tesque, eluent: A solution is 0.1% acetic acid, B solution is 0.1 % Acetic acid and 40% acetonitrile, gradient analysis of liquid A and liquid B, flow rate: 2 mL / min, UV detection at 210 nm. Sample 1 was dissolved in 0.1% acetic acid and 40% acetonitrile solution and filtered through a 0.45 μm membrane filter. The solution was subjected to HPLC, and repeated fractionation according to the fraction from which saponin was eluted to purify the Tsubakisponins specifically contained in Camellia. Moreover, fractionation was also performed except for the portion where the saponin was eluted. These were concentrated under reduced pressure and freeze-dried to obtain a powder of Tsubakisaponins (referred to as sample 6) and a powder of non-Tsubakisaponins (referred to as sample 7). Sample 6 contained camelia saponins A1, A2, B1, B2, C1 and C2.
これらの試料の血流量に及ぼす影響について、次のような試験を行った。６週齢のＩＣＲ系雄性マウス（日本クレア（株）から購入）を温度及び湿度の管理下、１２時間明暗サイクルで、餌料（日本クレア（株）製、ＣＥ−２）及び飲用水を自由摂取させて１週間予備飼育した。この後、１群１０匹として、対照群、試料１、試料６、試料７をそれぞれ投与する群の合計４群に群分けを行い、それぞれエーテル麻酔を行い、各試料を投与する前のマウスの足底の血流量を試験例１と同様の方法で測定した。次に、対照群には蒸留水を投与し、それ以外の群には各試料を５０ｍｇ／ｋｇ体重で経口投与し、３０分後、６０分後の血流量を同様に測定した。The following test was performed about the influence which these samples have on the blood flow rate. A 6-week-old ICR male mouse (purchased from Nippon Claire Co., Ltd.) under the control of temperature and humidity, with a 12-hour light / dark cycle, food (Nippon Claire Co., Ltd., CE-2) and drinking water are freely consumed. And pre-bred for 1 week. Thereafter, 10 mice per group were grouped into a total of 4 groups, each administered with the control group, sample 1, sample 6 and sample 7, each subjected to ether anesthesia, and each mouse before administration of each sample The blood flow at the sole of the foot was measured in the same manner as in Test Example 1. Next, distilled water was administered to the control group, and each sample was orally administered at 50 mg / kg body weight to the other groups, and the blood flow after 30 minutes and 60 minutes was measured in the same manner.
この結果を表３に示す。同表において、数値はそれぞれの被験物質を摂取する前の値を１００としたときの相対値として、平均値±標準偏差で表わした（ｎ＝１０、ＡＮＯＶＡ解析）。表３のデータから、対照群及び試料７を投与した群は、血流量に有意な変化は認められなかったが、試料１及び試料６を投与した群においては、３０分後、６０分後の両時間ともに、対照群に比べて血流量が有意に高かった。これにより、本発明に係る試料１及び試料６の摂取によって、血流量が増加することが明らかとなった。又、試料７には血流量を高める作用は見られず、試料６に血流量の増加作用が認められたことより、血流量を高める作用はツバキに特異的に含まれるツバキサポニン類によるものであることが強く示唆された。The results are shown in Table 3. In the table, the numerical values are expressed as average values ± standard deviation (n = 10, ANOVA analysis) as relative values when the value before taking each test substance is 100. From the data in Table 3, the control group and the group to which sample 7 was administered did not show a significant change in blood flow, but in the group to which sample 1 and sample 6 were administered, 30 minutes and 60 minutes later At both times, blood flow was significantly higher than the control group. Thereby, it became clear that blood flow volume increases by ingestion of sample 1 and sample 6 concerning the present invention. In addition, the effect of increasing blood flow was not observed in sample 7, and the effect of increasing blood flow was observed in sample 6. Therefore, the effect of increasing blood flow was due to Tsubakisponins specifically contained in camellia. It was strongly suggested that there was.
試験例３：血流の改善作用
試験に参加することに同意が得られた２５〜４０歳までの普段から冷え性を訴える女性３６名を被験者として、１群６名に群分けして、二重盲検法により試験を行った。まず、被験者を温度２４±２℃、湿度５０±１０％に制御した恒温・恒湿の部屋に入室させ、１０分間安静に待機させた。１０分後、右手の血流量を試験例１と同様の方法で測定し、その後、被験者の右手を水温１４℃の水をはった洗面器に５分間つける冷水負荷を行い、その直後に試料を摂取する前の右手の血流量を上記同様に測定した。次に、対照群には被験者が判別できないように着色したハードカプセルにデキストリンを充填したものを、その他の群にも同様に着色したハードカプセルに各試料を充填したものをそれぞれ水１００ｍＬとともに服用してもらい、１０分後、３０分後に再び前記と同様に右手の血流量を測定した。Test example 3: 36 women who complained of coldness from 25 to 40 years old who consented to participate in the blood flow improvement test were divided into 6 groups per group. The test was conducted in a blinded manner. First, the subject entered a room of constant temperature and humidity controlled at a temperature of 24 ± 2 ° C. and a humidity of 50 ± 10%, and was allowed to stand quietly for 10 minutes. Ten minutes later, the blood flow of the right hand was measured in the same manner as in Test Example 1, and then a cold water load was applied to the subject's right hand in a wash basin with a water temperature of 14 ° C. for 5 minutes. The blood flow of the right hand before ingestion was measured in the same manner as described above. Next, in the control group, the hard capsules that were colored so that the subject could not be identified were filled with dextrin, and in the other groups, the colored hard capsules that were filled with each sample were also taken with 100 mL of water. After 10 minutes and 30 minutes, the blood flow of the right hand was measured again in the same manner as described above.
この結果を表４に示す。同表において、数値はプラセボ及びそれぞれの被験物質を摂取する前の値を１００としたときの相対値として、平均値±標準偏差で表わした（ｎ＝６、ＡＮＯＶＡ解析）。表４のデータから、本発明に係るツバキ種子の脱脂物の水性成分は血流を速やかに回復させることが明らかとなった。The results are shown in Table 4. In the same table, the numerical values are expressed as average values ± standard deviation (n = 6, ANOVA analysis) as relative values when the value before taking the placebo and each test substance is 100. From the data of Table 4, it became clear that the aqueous component of the defatted camellia seeds according to the present invention quickly restores blood flow.
試験例４：育毛作用
試験に参加することに同意が得られた４０〜６５歳の頭頂部の髪が薄い５６名（男性：２８名、女性：２８名）を被験者として、１群８名に群分けして、二重盲検法により試験を行った。対照群には被験者が判別できないように着色したハードカプセルにデキストリンを充填したものを、その他の群には同様に着色したハードカプセルにそれぞれの試料を３００ｍｇ充填したものを毎日、６ヶ月間経口摂取させ、毛髪本数、毛髪成長速度及び毛髪径に及ぼす影響を評価した。それぞれの測定方法については、次のとおりである。毛髪本数については、デジタルマイクロスコープ（（株）ハイロックス製、ＫＨ−３０００）を用いて頭頂部の所定部位の拡大写真を撮影した後、１ｃｍ２あたりの毛髪数を測定した。毛髪成長速度については、まず、試験最終日より３日前に頭頂部の所定部位を毛刈りし、前記デジタルマイクロスコープにて拡大写真を撮影し、毛髪の長さを解析した。次に、毛刈りから７２時間後の毛の長さを上記同様の方法で測定した。７２時間で伸長した毛髪の長さを２４時間あたりに換算し、これを毛髪成長速度（μｍ／２４ｈｒ）とした。また、毛髪径については、所定部位の毛刈りを行った後、刈りとった毛髪を上記と同様に拡大写真を撮影し、毛髪の太さを解析した。尚、これらの測定項目はプラセボ及びそれぞれの試料を摂取させる前と、６ヶ月間摂取させた後に実施した。Test Example 4: 56 people (male: 28, female: 28) whose hair on the top of the 40-65-year-old who agreed to participate in the hair-growth action test was used as subjects. The test was conducted in a double-blind manner, divided into groups. The control group was filled with dextrin in a hard capsule colored so that the subject could not discriminate, and the other group was orally ingested in the same manner with a hard capsule filled with 300 mg of each sample for 6 months. The influence on the number of hairs, hair growth rate and hair diameter was evaluated. About each measuring method, it is as follows. About the number of hair, after taking the enlarged photograph of the predetermined | prescribed site | part of a head using a digital microscope (product made from Hylox Co., Ltd., KH-3000), the number of hair per cm < 2 > was measured. Regarding the hair growth rate, first, a predetermined part of the parietal region was shaved three days before the final test day, and an enlarged photograph was taken with the digital microscope, and the length of the hair was analyzed. Next, the length of hair 72 hours after hair cutting was measured by the same method as described above. The length of the hair extended in 72 hours was converted per 24 hours, and this was defined as the hair growth rate (μm / 24 hr). As for the hair diameter, after trimming a predetermined portion, the enlarged hair was taken in the same manner as described above, and the thickness of the hair was analyzed. These measurement items were performed before ingesting the placebo and each sample and after ingesting for 6 months.
この結果を表５に示す。同表において、数値はそれぞれの被験物質を６ヶ月間摂取した後の数値から、摂取する前の数値を引き、その変化を平均値±標準偏差で表わした（ｎ＝８、ＡＮＯＶＡ解析）。表５のデータから、プラセボを摂取した被験者の毛髪数、毛髪成長速度及び毛髪径に有意な変化はみられず、比較試料１を摂取した群は、毛髪数及び毛髪径に有意な差は認められなかったが、毛髪成長速度においてはプラセボ群と比べ有意に高値を示した。又、試料１〜５を摂取した群においては、プラセボを摂取した群よりも、毛髪数、毛髪成長速度及び毛髪径は有意に高値を示していた。これにより、本発明に係る試料を摂取することにより、発毛及び育毛効果を奏することが明らかとなった。又、その効果は比較試料１の場合よりも強かった。The results are shown in Table 5. In the same table, the numerical value was obtained by subtracting the numerical value before ingestion from the numerical value after ingesting each test substance for 6 months, and the change was expressed as an average value ± standard deviation (n = 8, ANOVA analysis). From the data in Table 5, there was no significant change in the number of hairs, hair growth rate and hair diameter of the subjects who took placebo, and there was a significant difference in the number of hairs and hair diameter in the group taking Comparative Sample 1. Although not, the hair growth rate was significantly higher than that of the placebo group. Moreover, in the group which ingested the samples 1-5, the number of hair, the hair growth rate, and the hair diameter showed the significantly high value compared with the group which ingested the placebo. Thereby, it became clear by taking the sample which concerns on this invention that there exists a hair growth and the hair growth effect. The effect was stronger than that of Comparative Sample 1.
試験例５：発毛促進作用及びその成分
発毛促進作用を有する実質的な有効成分を解明するために、試験例２において得られた試料６及び試料７を用いて、次のように試験を行った。毛周期が休止期にある８週齢のＣ３Ｈ／Ｈｅ系雄性マウス（日本クレア（株）から購入）４０匹の背部被毛をバリカンを用いて刈りとった。そのマウスを温度及び湿度の管理下、１２時間明暗サイクルで、飼料（日本クレア（株）製、ＣＥ−２）及び飲用水を自由摂取させて１週間予備飼育した。この後、１群１０匹として、対照群、試料１、試料６、試料７をそれぞれ投与する群の合計４群に群分けを行い、各試料を３０日間投与して発毛状態の観察を行った。Test Example 5: Hair growth promoting action and its components In order to elucidate the substantial active ingredient having hair growth promoting action, the test was carried out as follows using Sample 6 and Sample 7 obtained in Test Example 2. went. 40 back coats of 8 week old C3H / He male mice (purchased from Nippon Claire Co., Ltd.) whose hair cycle was in the resting phase were shaved using clippers. Under the control of temperature and humidity, the mice were preliminarily raised for 1 week by freely ingesting feed (manufactured by CLEA Japan, CE-2) and drinking water in a 12 hour light / dark cycle. Thereafter, as 10 animals per group, the control group, the sample 1, the sample 6 and the sample 7 were each administered into a total of 4 groups, and each sample was administered for 30 days to observe the hair growth state. It was.
この結果を表６に示す。同表において、数値は各群のマウス１０匹あたりの発毛個体数の比率（％）で表わした。表６のデータから、対照群及び試料７を投与した群では、発毛は認められなかったが、試料１及び試料６を投与した群においては、飼育１０日目から発毛するマウスが認められ、３０日目では８０％以上のマウスが発毛していた。又、試料７には発毛を促進する作用は見られず、試料６には発毛を促進する作用が認められたことより、発毛を促進する作用はツバキに特異的に含まれるツバキサポニン類によるものであることが強く示唆された。The results are shown in Table 6. In the same table, the numerical value was expressed as a ratio (%) of the number of hair growth individuals per 10 mice in each group. From the data in Table 6, no hair growth was observed in the control group and the group administered with the sample 7, but in the group administered with the sample 1 and the sample 6, mice that grow hair were observed from the 10th day of breeding. On the 30th day, 80% or more of the mice had hair growth. In addition, sample 7 showed no effect of promoting hair growth, and sample 6 was recognized to promote hair growth. Therefore, the effect of promoting hair growth was found to be specifically contained in camellia. It was strongly suggested that it was due to the kind.
試作例１
本発明の育毛剤としての試料１〜５のいずれか１種をカプセル充填機に供して、常法により１粒あたり内容量が２００ｍｇのゼラチン被覆ハードカプセル製剤を試作した。その他の試料についても同様に処理して５種類のゼラチン被覆ハードカプセル製剤を試作した。これらのカプセル製剤は経口摂取が可能な栄養補助食品、医薬品等として利用できる。Prototype example 1
Any one of Samples 1 to 5 as the hair-restoring agent of the present invention was subjected to a capsule filling machine, and a gelatin-coated hard capsule preparation having an inner volume of 200 mg per grain was prepared by a conventional method. The other samples were processed in the same manner to produce five types of gelatin-coated hard capsule formulations. These capsule preparations can be used as dietary supplements, medicines and the like that can be taken orally.
試作例２
本発明の育毛剤としての試料２：１５０部（質量基準。以下同様）、ミツロウ：４０部及び月見草油（英国エファモール社製）：８０部を約５０℃に加熱混合して均質にした後、カプセル充填機に供して、常法により１粒あたり内容量が２００ｍｇのゼラチン被覆ソフトカプセル製剤を試作した。このカプセル製剤は経口摂取可能な栄養補助食品として利用することができる。Prototype example 2
Sample 2: 150 parts (mass basis; the same applies hereinafter) as hair restorer of the present invention, beeswax: 40 parts and evening primrose oil (manufactured by Efamol Co., Ltd.): 80 parts after heating and mixing to about 50 ° C. to homogeneity, Using the capsule filling machine, a gelatin-coated soft capsule preparation having an inner volume of 200 mg per grain was prepared by a conventional method. This capsule preparation can be used as a dietary supplement that can be taken orally.
試作例３
本発明の育毛剤としての試料３：３０部、緑茶抽出物（ビーエイチエヌ（株）製）：０．５部、コーンスターチ（日本コーンスターチ（株）製）：１０５部、リン酸三カルシウム（米山化学工業（株）製）：５０部及びリボフラビン（ＤＳＭニュートリション・ジャパン（株）製）：７部を混合機に仕込み、１０分間攪拌混合した。この混合物を直打式打錠機に供して直径７ｍｍ、高さ４ｍｍ、質量１５０ｍｇ／個の素錠を作成し、ついでコーティング機でシェラック被膜を形成させて錠剤形状の食品を試作した。Prototype example 3
Sample 30:30 as a hair restorer of the present invention, green tea extract (manufactured by BN Co., Ltd.): 0.5 part, corn starch (manufactured by Nippon Corn Starch Co., Ltd.): 105 parts, tricalcium phosphate (Yoneyama Chemical Industries) (Made by Co., Ltd.): 50 parts and riboflavin (made by DSM Nutrition Japan Co., Ltd.): 7 parts were charged into a mixer and stirred and mixed for 10 minutes. This mixture was subjected to a direct compression tableting machine to prepare uncoated tablets having a diameter of 7 mm, a height of 4 mm, and a mass of 150 mg / piece, and then a shellac film was formed by the coating machine to produce a tablet-shaped food.
試作例４
市販の栄養ドリンク１００ｍＬに本発明の育毛剤としての試料４：２００ｍｇ加えて十分に混合し飲料を試作した。これは冷蔵庫で１年間保存しても外観及び風味に異状及び違和感は認められなかった。尚、本品は、血流促進及び／又は改善、冷え性改善、育毛等のために利用することができる。Prototype example 4
Sample 100: 200 mg as a hair restorer of the present invention was added to 100 mL of a commercially available nutrition drink and mixed well to prepare a beverage. Even if this was stored in a refrigerator for 1 year, no abnormalities or discomfort was observed in the appearance and flavor. In addition, this product can be used for blood flow promotion and / or improvement, cooling property improvement, hair growth and the like.
試作例５
即席麺の製造工程において、公知の原料に本発明の育毛剤としての試料１を３００ｍｇ加えて即席麺を試作した。これは常温で６ヵ月間保存しても外観及び風味に異状及び違和感は認められなかった。尚、本品は、育毛のために利用することができる。Prototype example 5
In the instant noodle manufacturing process, 300 mg of sample 1 as the hair restorer of the present invention was added to a known raw material to produce instant noodles. Even when it was stored at room temperature for 6 months, no abnormality or discomfort was observed in appearance and flavor. This product can be used for hair growth.
本発明の、ツバキ科ツバキ属に属するツバキ（Ｃａｍｅｌｌｉａ ｊａｐｏｎｉｃａ）の実及び／又は種子の脱脂粕の水性成分を有効成分として含有してなる育毛剤は、これを経口摂取することにより全身の血流を促進及び／又は改善して発毛促進、育毛及び／又は養毛の作用を有するため、飲食品、医薬品、医薬部外品、飼料等の分野において有効利用できる。According to the present invention, the hair restorer containing the aqueous component of camellia (Camellia japonica) fruit and / or seed defatted cocoon as an active ingredient of the camellia genus Camellia belongs to the systemic blood flow. Promotes and / or improves hair growth, has the effects of promoting hair growth, hair growth and / or hair nourishment, and thus can be effectively used in the fields of foods and drinks, pharmaceuticals, quasi drugs, and feeds.
Claims (5)
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| JP2009136689A JP5597828B2 (en) | 2009-05-15 | 2009-05-15 | Hair restorer |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101914158B1 (en) * | 2012-01-10 | 2018-11-02 | (주)아모레퍼시픽 | Agent for promoting hair growth comprising saponin of Torreya seed |
| WO2020009147A1 (en) | 2018-07-04 | 2020-01-09 | 正典 佐伯 | Stem cell filtrate formulation and method for preparing same |
| WO2021215882A1 (en) * | 2020-04-24 | 2021-10-28 | 바이오스펙트럼 주식회사 | Composition for preventing hair loss or promoting hair growth comprising camellia pericarp extract as active ingredient |
| KR102359447B1 (en) * | 2021-03-09 | 2022-02-09 | (주)아모레퍼시픽 | Composition for promoting hair growth |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20230052470A (en) | 2021-10-13 | 2023-04-20 | (주)아모레퍼시픽 | Composition for promoting hair growth |
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| WO2021215882A1 (en) * | 2020-04-24 | 2021-10-28 | 바이오스펙트럼 주식회사 | Composition for preventing hair loss or promoting hair growth comprising camellia pericarp extract as active ingredient |
| KR102359447B1 (en) * | 2021-03-09 | 2022-02-09 | (주)아모레퍼시픽 | Composition for promoting hair growth |
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| JP5597828B2 (en) | 2014-10-01 |
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