JP2010078580A - Analyzing device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an analyzing device capable of shortening a sucking time of a fixed quantity of sample liquid. <P>SOLUTION: An opening part (101) of a capillary channel 11 for supply is opened on a tilted surface (14) of the chip of a spot contact part (103). Since sampling is performed in the attitude wherein the tilted surface is tilted along a finger chip of an examinee, an influence of gravity when sucking the sample liquid by a capillary force can be reduced, and sucking speed is heightened, to thereby enable sampling of a prescribed quantity of the sample liquid in a short time. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to an analytical device used for analyzing a liquid collected from a living organism.

Conventionally, as a method for analyzing a liquid collected from a living organism or the like, a method for analyzing using a device for analysis in which a liquid channel is formed is known. The analytical device can control the fluid using a rotating device, and utilizes centrifugal force to dilute the sample liquid, measure the solution, separate the solid component, transfer and distribute the separated fluid, Since a solution and a reagent can be mixed, various biochemical analyzes can be performed.
JP 7-500910 (Fig. 1)

  If the analysis device is classified according to the method of taking the sample liquid, in addition to the type in which an appropriate amount is injected by a syringe as seen in Patent Document 1, the opening 101 of the capillary channel is used as a sample liquid reservoir as shown in FIG. A type that is brought into contact and sucked up by capillary force is conceivable.

  The analysis device 100 shown in FIG. 12 is provided in a spotting portion 103 in which an opening 101 is formed so as to protrude from the analysis device main body 102. As shown in FIG. 13, the analytical device 100 is configured by bonding a base substrate 1 and a cover substrate 2 together.

  In the base substrate 1, internal recesses to be a holding chamber 3, a reagent chamber 4, a flow path 5, a measurement chamber 6, and a flow path 7 are formed on the bonding surface 1 a with the cover substrate 2. The reagent chamber 4 carries an analysis reagent (not shown). Each opening surface of the internal recess is closed by the cover substrate 2 to form a cavity having a gap of a predetermined size, transfer of the sample liquid by capillary force, hold a predetermined liquid volume, etc. The function is supposed to work. Reference numeral 8b denotes an air opening hole formed on the cover substrate 2 corresponding to the position of the outlet 8a on the base substrate 1 side.

  The spotting portion 103 is formed by joining the protrusion 9 of the base substrate 1 and the protrusion 10 of the cover substrate 2, and the protrusion length L 1 of the protrusion 9 from the analysis device body 102 and the analysis device body 102. The protrusion length L2 of the protrusion 10 is formed to be the same. The tip of the spotting portion 103 and the holding chamber 3 are connected by a supply capillary channel 11 formed between the base substrate 1 and the cover substrate 2 as shown in FIGS. .

  When blood is analyzed as a sample solution, the posture of the analysis device 100 is vertical as shown in FIG. 16, and the spotting portion 103 is brought into contact with the blood pool 13 of the fingertip 12 of the examinee, The blood as the sample is sucked up to the holding chamber 3 by the capillary force of the supply capillary channel 11 and the holding chamber 3.

  However, when the speed of the blood sucked up depends on the posture of the supply capillary channel 11 and the holding chamber 3, and it takes a short time to contact the spotting portion 103 with the blood reservoir 13, or the posture is inappropriate. However, there is a problem that it is not possible to sample only a fixed amount of blood necessary for performing an accurate analysis.

  An object of the present invention is to provide an analytical device that can reduce the time required to suck up a fixed amount of sample liquid in an analytical device that is brought into contact with a sample liquid reservoir and sucked up by capillary force.

  In the analysis device according to claim 1 of the present invention, one end of the supply capillary channel is opened at a spotted portion formed to protrude from the analysis device body, and the supply capillary channel is the analysis device body. The sample solution is connected to the microchannel structure formed inside, and sucked up by the capillary force of the supply capillary channel and used for reading to access the sucked solution The device is characterized in that the tip of the spotted portion is formed on an inclined surface, and the one end of the supply capillary channel is open on the inclined surface.

The analysis device according to claim 2 of the present invention is characterized in that, in claim 1, a clogging prevention concave portion communicating with the one end of the supply capillary channel is formed on the inclined surface.
The analysis device according to claim 3 of the present invention is the analysis device body according to claim 1, wherein the analysis device main body formed by projecting the spotted portion includes a base substrate on which an internal recess serving as the microchannel structure is formed, And a cover substrate that is bonded to the base substrate and closes the opening surface of the internal recess, and the protruding length of the base substrate that forms the spotted portion is the same as that of the cover substrate that forms the spotted portion. It is shorter than the projecting length, and the width of the cover substrate that forms the spotted portion is narrower than the width of the base substrate that forms the spotted portion.

  The analysis device according to claim 4 of the present invention is characterized in that in any one of claims 1 to 3, the angle of the inclined surface at the tip of the spotting portion is 30 ° to 45 °. .

  According to this configuration, the tip of the spotted portion is formed on an inclined surface, and the one end of the supply capillary channel is opened on the inclined surface, so that the inclined surface of the tip of the spotted portion is at the fingertip of the examinee. In this case, the sample is sampled in a slanted posture, and the influence of gravity when sucking up the sample liquid due to capillary force can be reduced. Sampling is possible.

Hereinafter, the analysis device 101A of the present invention will be described based on each embodiment shown in FIGS.
(Embodiment 1)
1 to 6 show Embodiment 1 of the present invention.

In addition, the same code | symbol is attached | subjected and demonstrated to what performs the effect | action similar to FIGS.
As shown in FIGS. 1 and 2, the analysis device 100 </ b> A configured by bonding the base substrate 1 and the cover substrate 2 has the tip of the spotting portion 103 formed by the inclined surface 14. The point that one end of the supply capillary channel 11 is open on the surface 14 is different from the comparative example shown in FIGS.

  As shown in FIG. 2, since the tip of the spotting portion 103 is formed on the inclined surface 14, the spotting portion 103 formed by joining the projection 9 of the base substrate 1 and the projection 10 of the cover substrate 2. The protrusion length L2 of the protrusion 10 is shorter than the protrusion length L1 of the protrusion 9. Moreover, as shown in FIG. 3, the angle θ of the inclined surface 14 is an acute angle. Specifically, when the sample liquid is blood, it is preferably 30 ° to 45 °. FIGS. 3 and 4 show a state in which the opening 101 which is one end of the supply capillary channel 11 is opened at the inclined surface 14.

  In the first embodiment, the width W1 of the protrusion 9 of the base substrate 1 near the opening 101 and the width W2 of the protrusion 10 of the cover substrate 2 near the opening 101 are formed the same.

  Further, the supply capillary channel 11, the holding chamber 3, and the walls of the channels 5 and 7 are subjected to hydrophilic treatment. Examples of the hydrophilic treatment method include a surface treatment method using an active gas such as plasma, corona, ozone, and fluorine, and a surface treatment using a surfactant or a hydrophilic polymer. Here, hydrophilicity means that the contact angle with water is less than 90 °.

  The specific size of the analysis device 100A is such that when the thickness of the base substrate 1 is 15 mm, the thickness of the cover substrate 2 is 1 mm, and the analysis device 100A is configured with approximately 80 mm square, the depth of the holding chamber 3 is 0. .1 mm. The depth of the reagent chamber 4 is 0.3 mm to 0.5 mm, which is deeper than the depth of the holding chamber 3. By setting in this way, the blood injected into the holding chamber 3 does not proceed to the reagent chamber 4 only by the capillary force, and the sample solution is obtained by utilizing the centrifugal force obtained by rotating the analysis device 100A. This is for transportation.

  The supply capillary channel 11, the holding chamber 3, the channel 5, and the channel 7 are formed with a depth of 0.02 mm to less than 0.3 mm. If the sample liquid flows by capillary force, this dimension is used. It is not limited to. In general, since liquid such as blood is measured and analyzed, 0.02 mm to less than 0.3 mm is desirable. Moreover, although the depth of the reagent chamber 4 and the measurement chamber 6 is formed at 0.3 mm to 0.5 mm, this is based on conditions for measuring the amount of sample solution and absorbance (optical path length, measurement wavelength). The reaction concentration of the sample solution, the type of reagent, etc.). Then, the sample liquid transferred to the measurement chamber 6 is optically measured.

  The width W1 of the protrusion 9 of the base substrate 1 and the width W2 of the protrusion 10 of the cover substrate 2 are 3 to 5 mm, and the protruding length L3 (= L1) of the spotted portion 103 from the analysis device body 102 is 5 mm.

  With this configuration, when blood is analyzed as a sample solution, the posture is vertical and the tip of the spotted portion 103 is placed at the fingertip of the examinee as in the analysis device 100A indicated by the phantom line in FIG. Since the opening 101 at one end of the supply capillary channel 11 opened at the inclined surface 14 does not contact the blood reservoir 13 even if it is brought into contact with the blood reservoir 13, the supply chamber from the supply capillary channel 11 is maintained. 3 is not sucking up blood.

  Therefore, when the analysis device 100A is tilted as shown by the solid line in FIG. 5 and the inclined surface 14 is moved along the fingertip 12, the opening 101 opened at the inclined surface 14 contacts the blood reservoir 13, Blood is sucked into the holding chamber 3 from the supply capillary channel 11.

  Since the tip shape of the spotting portion 101 is formed on the inclined surface 14 as described above, the length of the supply capillary channel 11 is longer than the distance shown in FIG. 6 compared to the comparative example shown in FIG. The length of the capillary channel 11 for supply and the holding chamber 3 at the time of suction is 30 ° to 45 °, which is the same as the angle θ of the inclined surface 14, and the comparative example shown in FIG. As compared with the case where the angle between the supply capillary channel 11 and the holding chamber 3 is vertical as in the case of the above, it is possible to reduce the magnitude of gravity that affects the speed of the sucked blood and to hold a fixed amount of blood. 3, the time required for sampling can be shortened compared with the comparative example of FIG.

  As a result, it is possible to reduce the occurrence of an insufficient state where the blood held in the holding chamber 3 does not reach a fixed amount, and the blood held in the holding chamber 3 is transferred toward the measurement chamber 6 by centrifugal force. When the solution in the measurement chamber 6 is optically accessed and analyzed, accurate analysis can be performed.

(Embodiment 2)
7 and 8 show a second embodiment of the present invention.
In the case of the first embodiment, when the analysis device 100A is pressed too much against the fingertip 12 of the examinee as shown in FIG. 8 (b), the opening 101 opened at the inclined surface 14 is formed by the fingertip 12. It is conceivable that the blood sucking speed is reduced due to obstruction. On the other hand, the second embodiment is different from the first embodiment in that an obstruction prevention recess 15 communicating with the opening 101 is formed on the inclined surface 14 as shown in FIG. Specifically, the cover substrate 2 is the same as that of the first embodiment, but the blocking prevention recess 15 is formed in the base substrate 1.

  With this configuration, even when the analysis device 100A is pressed too much against the fingertip 12 of the examinee, the occlusion prevention recess 15 prevents the fingertip 12 from contacting the opening 101 as shown in FIG. In this case, the blood suction speed does not decrease.

(Embodiment 3)
9 to 11 show Embodiment 3 of the present invention.
In Embodiment 1, since the inclined surface 14 is formed in the spotting portion 103, when the inclined surface 14 is brought into contact with the blood reservoir 13, the area wetted by blood becomes larger than that in the comparative example shown in FIG. The blood that cannot be sucked into the supply capillary channel 11 by the capillary force remains at the tip of the base substrate 1 and is solidified there. In the third embodiment, the blood remaining at the tip of the base substrate 1 can be reduced. .

  In the first embodiment, the width W1 of the protrusion 9 of the base substrate 1 and the width W2 of the protrusion 10 of the cover substrate 2 are formed to be the same. In this third embodiment, the inclined surface 14 of the spotting portion 103 is formed. The width W2 near the opening 101 of the protrusion 10 of the cover substrate 2 is formed narrower than the width W1 of the base end of the protrusion 9 of the base substrate 1. In FIG. 9, the tip of the protrusion 10 of the cover substrate 2 is located near the center of the protrusion 9 of the base substrate 1, and the one end of the supply capillary channel 11 is connected to both sides 10R, 10R of the protrusion 10 as shown in FIG. 10L and the tip 10T of the protrusion 10 are open.

  11A, the blood 13a remaining on the protrusion 9 of the base substrate 1 starts to be sucked into the holding chamber 3 via the supply capillary channel 11 by the capillary force as shown in FIG. The base substrate 1 of the base substrate 1 is supplied from the supply capillary channel 11 formed between the tip of the protrusion 10 of the cover substrate 2 whose tip is narrower than the protrusion 9 of the substrate 1 and the protrusion 9 of the base substrate 1. As shown in FIG. 11B, most of the blood 13a remaining on the inclined surface 14 of the projection 9 can be sucked up by the capillary force.

  In each of the above-described embodiments, the analysis device 100A can be tilted in the horizontal direction as the inclination of the spotted portion becomes an acute angle, which is effective in shortening the filling time. The effect of the inclination of the spotting part 103 when the sample liquid is blood is confirmed to be in the range of 30 to 45 °. However, if the sample solution has an effect on the filling time even at 45 ° or more, it is limited to this angle. It is not something.

  In each of the above embodiments, the case of an analytical device used for reading optically accessing a solution in the measurement chamber 6 has been described as an example. However, an electrochemical sensor is provided in the measurement chamber 6 to provide the solution with the solution. The same applies to the analytical device used for reading to access.

  In each of the above embodiments, the case where the sample liquid sucked into the holding chamber 3 by capillary force is transferred to the measurement chamber 6 by centrifugal force has been described as an example. However, the opening is provided in the measurement chamber having capillary force. In the case of an analytical device used for reading a sample liquid directly from the unit 101 and optically or electrically accessing a test object entering the measurement chamber, the tip shape of the spotting unit 103 is inclined. By forming on the surface 14, the above-mentioned fixed amount of sample liquid can be sampled and an accurate analysis can be realized.

  The analytical device of the present invention is useful for measuring components of biological fluids with an electrochemical sensor or an optical sensor.

FIG. 3 is an external perspective view of the analysis device according to (Embodiment 1) of the present invention. Exploded perspective view of the same embodiment Enlarged view of the main part of the same embodiment The top view of the principal part of the same embodiment Usage state explanatory diagram of the same embodiment Enlarged view of the usage state of the same embodiment The expanded perspective view of the principal part of the analysis device of (Embodiment 2) of this invention The expanded sectional view of the use condition of the same embodiment, and the expanded sectional view of the use condition of (Embodiment 1) The expanded perspective view of the principal part of the analysis device of (Embodiment 3) of this invention The top view of the principal part of the same embodiment Plan view of usage state of the embodiment External perspective view of an analytical device of the type in which the opening of the capillary channel is brought into contact with the sample liquid reservoir and sucked up by capillary force 12 is an exploded perspective view of the comparative example shown in FIG. Enlarged view of the main part of the comparative example Plan view of the main part of the comparative example Usage state explanatory diagram of the comparative example

Explanation of symbols

DESCRIPTION OF SYMBOLS 100A Analysis device 101 Opening part 102 Analysis device main body 103 Spotting part L1 Projection length of protrusion 9 L2 Projection length of protrusion 10 W2 Width of protrusion 10 W1 Width of protrusion 9 1 Base substrate 1a Cover substrate 2 of base substrate 2 cover substrate 3 holding chamber 4 reagent chamber 5 flow channel 6 measurement chamber 7 flow channel 8a outlet on the base substrate 1 side 8b air release hole 9 projection on the base substrate 1 10 projection on the cover substrate 2 10R, 10L Both sides of the protrusion 10 10T The tip of the protrusion 10 11 Capillary flow channel for supply 12 Fingertip 14 of the examinee 14 Inclined surface 15 Blocking prevention recess θ Angle of the inclined surface 14

Claims (4)

One end of a supply capillary channel is opened at a spotted portion formed projecting from the analysis device body, and the supply capillary channel is connected to a microchannel structure formed inside the analysis device body, An analytical device used for reading to suck up the sample solution attached to the spotting portion with the capillary force of the supply capillary channel and to access the sucked solution,
An analysis device in which a tip of the spotting portion is formed on an inclined surface, and the one end of the supply capillary channel is opened on the inclined surface. The analysis device according to claim 1, wherein a clogging prevention concave portion communicating with the one end of the supply capillary channel is formed on the inclined surface. The analysis device main body formed by protruding the spotted portion is:
A base substrate having an internal recess to be the microchannel structure;
A cover substrate that is bonded to the base substrate and closes an opening surface of the internal recess,
The protruding length of the base substrate that forms the spotted portion is shorter than the protruding length of the cover substrate that forms the spotted portion, and the width of the cover substrate that forms the spotted portion is the spotted portion. The analytical device according to claim 1, wherein the analytical device is narrower than a width of the base substrate forming the substrate. The analysis device according to any one of claims 1 to 3, wherein an angle of the inclined surface at a tip of a spotting portion is 30 ° to 45 °.

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