JP2009513141A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2009513141A5 JP2009513141A5 JP2008538055A JP2008538055A JP2009513141A5 JP 2009513141 A5 JP2009513141 A5 JP 2009513141A5 JP 2008538055 A JP2008538055 A JP 2008538055A JP 2008538055 A JP2008538055 A JP 2008538055A JP 2009513141 A5 JP2009513141 A5 JP 2009513141A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- detection
- protein
- acid binding
- construct
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000007523 nucleic acids Chemical group 0.000 claims 57
- 108020004707 nucleic acids Proteins 0.000 claims 53
- 102000039446 nucleic acids Human genes 0.000 claims 53
- 238000001514 detection method Methods 0.000 claims 48
- 238000000034 method Methods 0.000 claims 35
- 239000012634 fragment Substances 0.000 claims 33
- 102000004169 proteins and genes Human genes 0.000 claims 31
- 108090000623 proteins and genes Proteins 0.000 claims 31
- 108090000765 processed proteins & peptides Proteins 0.000 claims 20
- 229920001184 polypeptide Polymers 0.000 claims 19
- 102000004196 processed proteins & peptides Human genes 0.000 claims 19
- 210000004027 cell Anatomy 0.000 claims 9
- 150000001413 amino acids Chemical class 0.000 claims 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 7
- 201000010099 disease Diseases 0.000 claims 5
- 108010048367 enhanced green fluorescent protein Proteins 0.000 claims 5
- 108091023037 Aptamer Proteins 0.000 claims 4
- 108090000204 Dipeptidase 1 Proteins 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 4
- 108090000790 Enzymes Proteins 0.000 claims 4
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims 4
- 102000006635 beta-lactamase Human genes 0.000 claims 4
- 108091006047 fluorescent proteins Proteins 0.000 claims 4
- 102000034287 fluorescent proteins Human genes 0.000 claims 4
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 108020004414 DNA Proteins 0.000 claims 3
- 102000004144 Green Fluorescent Proteins Human genes 0.000 claims 3
- 108091005948 blue fluorescent proteins Proteins 0.000 claims 3
- 230000000295 complement effect Effects 0.000 claims 3
- 108010082025 cyan fluorescent protein Proteins 0.000 claims 3
- 208000035475 disorder Diseases 0.000 claims 3
- 239000005090 green fluorescent protein Substances 0.000 claims 3
- 238000001727 in vivo Methods 0.000 claims 3
- 230000009261 transgenic effect Effects 0.000 claims 3
- 108091005957 yellow fluorescent proteins Proteins 0.000 claims 3
- 241000894006 Bacteria Species 0.000 claims 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 2
- 108010022394 Threonine synthase Proteins 0.000 claims 2
- 239000012472 biological sample Substances 0.000 claims 2
- 102000004419 dihydrofolate reductase Human genes 0.000 claims 2
- 230000004927 fusion Effects 0.000 claims 2
- 210000004962 mammalian cell Anatomy 0.000 claims 2
- 244000052769 pathogen Species 0.000 claims 2
- 230000001717 pathogenic effect Effects 0.000 claims 2
- 239000013612 plasmid Substances 0.000 claims 2
- 230000035945 sensitivity Effects 0.000 claims 2
- 102100026189 Beta-galactosidase Human genes 0.000 claims 1
- 101710132601 Capsid protein Proteins 0.000 claims 1
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 claims 1
- 101710094648 Coat protein Proteins 0.000 claims 1
- 241000252212 Danio rerio Species 0.000 claims 1
- 101100232687 Drosophila melanogaster eIF4A gene Proteins 0.000 claims 1
- 108091005941 EBFP Proteins 0.000 claims 1
- 108091005942 ECFP Proteins 0.000 claims 1
- 241000233866 Fungi Species 0.000 claims 1
- 102000053187 Glucuronidase Human genes 0.000 claims 1
- 108010060309 Glucuronidase Proteins 0.000 claims 1
- 102000005720 Glutathione transferase Human genes 0.000 claims 1
- 108010070675 Glutathione transferase Proteins 0.000 claims 1
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 claims 1
- 101710125418 Major capsid protein Proteins 0.000 claims 1
- 241000699670 Mus sp. Species 0.000 claims 1
- 101710141454 Nucleoprotein Proteins 0.000 claims 1
- 102000035195 Peptidases Human genes 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 claims 1
- 101710083689 Probable capsid protein Proteins 0.000 claims 1
- 241000700605 Viruses Species 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 108010005774 beta-Galactosidase Proteins 0.000 claims 1
- 102000006995 beta-Glucosidase Human genes 0.000 claims 1
- 108010047754 beta-Glucosidase Proteins 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 210000002919 epithelial cell Anatomy 0.000 claims 1
- 210000002950 fibroblast Anatomy 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 206010022000 influenza Diseases 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 210000002569 neuron Anatomy 0.000 claims 1
- 210000000287 oocyte Anatomy 0.000 claims 1
- 244000045947 parasite Species 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 238000012797 qualification Methods 0.000 claims 1
- 108010054624 red fluorescent protein Proteins 0.000 claims 1
- 230000007017 scission Effects 0.000 claims 1
- 108091006106 transcriptional activators Proteins 0.000 claims 1
- 238000001890 transfection Methods 0.000 claims 1
- 230000009466 transformation Effects 0.000 claims 1
- 210000004881 tumor cell Anatomy 0.000 claims 1
- 238000011179 visual inspection Methods 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US73074605P | 2005-10-27 | 2005-10-27 | |
| PCT/US2006/042210 WO2007050979A2 (en) | 2005-10-27 | 2006-10-27 | Real time nucleic acid detection in vivo using protein complementation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2009513141A JP2009513141A (ja) | 2009-04-02 |
| JP2009513141A5 true JP2009513141A5 (enExample) | 2009-12-17 |
Family
ID=37968606
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008538055A Pending JP2009513141A (ja) | 2005-10-27 | 2006-10-27 | タンパク質相補によるリアルタイムインビボ核酸検出 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20090029370A1 (enExample) |
| EP (1) | EP1954828A2 (enExample) |
| JP (1) | JP2009513141A (enExample) |
| KR (1) | KR20080070837A (enExample) |
| CN (1) | CN101365805A (enExample) |
| AU (1) | AU2006305901A1 (enExample) |
| CA (1) | CA2627410A1 (enExample) |
| IL (1) | IL191064A0 (enExample) |
| WO (1) | WO2007050979A2 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7662554B2 (en) | 2002-10-09 | 2010-02-16 | The Trustees Of Boston University | Nucleic acid supported protein complementation |
| KR20080070838A (ko) * | 2005-10-27 | 2008-07-31 | 트러스티스 오브 보스턴 유니버시티 | 활성화된 분절폴리펩티드 및 이의 생산 방법 및 이의 용도 |
| RU2009120007A (ru) * | 2006-10-27 | 2010-12-10 | Трастиз Оф Бостон Юниверсити (Us) | Направленные конъюгаты с разделенными биомолекулами для лечения заболеваний, злокачественных образований и нарушений и способы их получения |
| CN101889074A (zh) * | 2007-10-04 | 2010-11-17 | 哈尔西恩莫尔丘勒公司 | 采用电子显微镜对核酸聚合物测序 |
| WO2011135040A1 (en) | 2010-04-30 | 2011-11-03 | F. Hoffmann-La Roche Ag | Fluorescent antibody fusion protein, its production and use |
| CN106868157B (zh) * | 2017-03-17 | 2019-08-27 | 广东省生态环境技术研究所 | 一种金黄色葡萄球菌的检测方法及检测试剂盒 |
| CN106868158B (zh) * | 2017-03-17 | 2019-08-27 | 广东省生态环境技术研究所 | 一种沙门氏菌的检测方法及检测试剂盒 |
| EP3655530A4 (en) * | 2017-07-17 | 2021-07-28 | The Broad Institute, Inc. | NEW TYPE VI CRISPR ORTHOLOGIST AND SYSTEMS |
| CN108267595A (zh) * | 2017-11-27 | 2018-07-10 | 南京天纵易康生物科技股份有限公司 | 一种基于双分子荧光互补技术的Myo检测试剂盒、制备及使用方法 |
| US20210348217A1 (en) * | 2018-07-16 | 2021-11-11 | Massachusetts Institute Of Technology | Rna tickertape for recording transcriptional histories of cells |
| WO2021010442A1 (ja) * | 2019-07-16 | 2021-01-21 | 国立大学法人東京大学 | タンパク質とｒｎａとの相互作用評価方法、相互作用調節物質評価方法、及び相互作用調節物質検出方法、並びに、これらに用いる融合タンパク質、キット、及びバイオセンサ |
| CN110455756B (zh) * | 2019-07-26 | 2022-03-29 | 重庆工商大学 | 一种同时检测二价铅离子和二价铜离子的方法 |
| CN114252622A (zh) * | 2020-09-23 | 2022-03-29 | 中国科学院大连化学物理研究所 | 一种体外筛选新型冠状病毒抑制剂的方法 |
| CN113092461B (zh) * | 2021-04-09 | 2023-08-22 | 四川大学华西医院 | 一种均相二维可视化/荧光分析方法及应用 |
| CN113504274B (zh) * | 2021-07-20 | 2023-11-21 | 郑州轻工业大学 | 一种共价有机骨架材料及其制备方法和应用，适配体传感器及其制备方法 |
| CN115855920B (zh) * | 2022-08-29 | 2026-04-14 | 江苏科技大学 | 智能手机APP结合DSN酶微流体芯片比色检测miRNA-21的方法 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2075858C (en) * | 1991-08-15 | 1998-05-19 | Scott J. Eisenbeis | Detection of complementary nucleotide sequences |
| JPH08500965A (ja) * | 1992-05-22 | 1996-02-06 | デイナ ファーバー キャンサー インスチチュート | ハイブリッドウイルス発現ベクター，その使用および新規アッセイ |
| AU7401394A (en) * | 1993-07-20 | 1995-02-20 | University Of Massachusetts Medical Center | In vivo nucleic acid hybridization method |
| US5854033A (en) * | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| CA2196496A1 (en) * | 1997-01-31 | 1998-07-31 | Stephen William Watson Michnick | Protein fragment complementation assay for the detection of protein-protein interactions |
| US6294330B1 (en) * | 1997-01-31 | 2001-09-25 | Odyssey Pharmaceuticals Inc. | Protein fragment complementation assays for the detection of biological or drug interactions |
| EP0878552A1 (en) * | 1997-05-13 | 1998-11-18 | Erasmus Universiteit Rotterdam | Molecular detection of chromosome aberrations |
| US7166424B2 (en) * | 1998-02-02 | 2007-01-23 | Odyssey Thera Inc. | Fragments of fluorescent proteins for protein fragment complementation assays |
| US6287772B1 (en) * | 1998-04-29 | 2001-09-11 | Boston Probes, Inc. | Methods, kits and compositions for detecting and quantitating target sequences |
| JP2002518047A (ja) * | 1998-06-23 | 2002-06-25 | メディカル リサーチ カウンシル | 構造化されたアンチセンス核酸分子 |
| US8148110B2 (en) * | 1999-03-15 | 2012-04-03 | The Board Of Trustees Of The Leland Stanford Junior University | Detection of molecular interactions by β-lactamase reporter fragment complementation |
| US7306904B2 (en) * | 2000-02-18 | 2007-12-11 | Olink Ab | Methods and kits for proximity probing |
| AU781992B2 (en) * | 2000-04-04 | 2005-06-23 | Medical Research Council | Methods involving induced dimerisation by cellular components |
| US6951947B2 (en) * | 2000-07-13 | 2005-10-04 | The Scripps Research Institute | Labeled peptides, proteins and antibodies and processes and intermediates useful for their preparation |
| GB0226727D0 (en) * | 2002-11-15 | 2002-12-24 | Medical Res Council | Intrabodies |
| US7662554B2 (en) * | 2002-10-09 | 2010-02-16 | The Trustees Of Boston University | Nucleic acid supported protein complementation |
| GB0226729D0 (en) * | 2002-11-15 | 2002-12-24 | Medical Res Council | Intracellular antibodies |
| CA2607104A1 (en) * | 2005-05-05 | 2006-11-16 | The Arizona Board Of Regents On Behalf Of The University Of Arizona | Sequence enabled reassembly (seer) - a novel method for visualizing specific dna sequences |
| KR20080070838A (ko) * | 2005-10-27 | 2008-07-31 | 트러스티스 오브 보스턴 유니버시티 | 활성화된 분절폴리펩티드 및 이의 생산 방법 및 이의 용도 |
-
2006
- 2006-10-27 AU AU2006305901A patent/AU2006305901A1/en not_active Abandoned
- 2006-10-27 EP EP06827001A patent/EP1954828A2/en not_active Withdrawn
- 2006-10-27 JP JP2008538055A patent/JP2009513141A/ja active Pending
- 2006-10-27 WO PCT/US2006/042210 patent/WO2007050979A2/en not_active Ceased
- 2006-10-27 CA CA002627410A patent/CA2627410A1/en not_active Abandoned
- 2006-10-27 CN CNA2006800498010A patent/CN101365805A/zh active Pending
- 2006-10-27 KR KR1020087012697A patent/KR20080070837A/ko not_active Withdrawn
- 2006-10-27 US US12/091,682 patent/US20090029370A1/en not_active Abandoned
-
2008
- 2008-04-27 IL IL191064A patent/IL191064A0/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2009513141A5 (enExample) | ||
| Pfeiffer et al. | Refinement of tools for targeted gene expression in Drosophila | |
| AU2022291617B2 (en) | High throughput assay for measuring adenovirus replication kinetics | |
| CN104109712A (zh) | 使用信号探针的方法和材料 | |
| Sachsenhauser et al. | Directed evolution to improve protein folding in vivo | |
| KR20020059370A (ko) | 융합 라이브러리의 제작 및 사용을 위한 방법 및 조성물 | |
| JP2019106996A (ja) | タンパク質の安定性を測定する方法およびその使用 | |
| Brasino et al. | Real-time femtomolar detection of cancer biomarkers from photoconjugated antibody–phage constructs | |
| US9400249B2 (en) | Detection of biopolymer interactions, cancer cells, and pathogens using split-supercharged GFP | |
| Jin et al. | Visualization of human immunodeficiency virus protease inhibition using a novel Förster resonance energy transfer molecular probe | |
| Cullen | [11] Utility of the secreted placental alkaline phosphatase reporter enzyme | |
| JP2012127694A (ja) | 細胞内ユビキチン化の検出方法 | |
| Furman et al. | Systematic evaluation of split-fluorescent proteins for the direct detection of native and methylated DNA | |
| AU1822800A (en) | Methods for validating polypeptide targets that correlate to cellular phenotypes | |
| US20030039990A1 (en) | Non-destructive cell-based assay | |
| Prajapati et al. | Hitchhiking on condensed chromatin promotes plasmid persistence in yeast without perturbing chromosome function | |
| US8232070B2 (en) | DNP63A gene and screening methods of anticancer agent by using it | |
| KR20250004513A (ko) | Bret-기반 rab11a 활성 바이오센서 및 이를 포함하는 세포주 | |
| Hodgson | New approaches to in-cell detection of protein activity: genetically encoded chemiluminescence probes pave the way to robust HTS assays | |
| JP2026509984A (ja) | Ｒループセンサー | |
| Bignon et al. | In Vivo Protein–Protein Binding Competition Assay Based on Split-GFP Reassembly: Proof of Concept. Biomolecules 2023, 13, 354 | |
| KR101576363B1 (ko) | 신호가 증폭된 형광 편광 측정방법 | |
| Harnois | New tool for late stage visualization of Oskar protein in Drosophila melanogaster oocytes | |
| TW201031757A (en) | Methods and systems for identifying polynucleotide sequences with translational self-cleavage activity |