JP2009195239A5 - - Google Patents
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- Publication number
- JP2009195239A5 JP2009195239A5 JP2009104135A JP2009104135A JP2009195239A5 JP 2009195239 A5 JP2009195239 A5 JP 2009195239A5 JP 2009104135 A JP2009104135 A JP 2009104135A JP 2009104135 A JP2009104135 A JP 2009104135A JP 2009195239 A5 JP2009195239 A5 JP 2009195239A5
- Authority
- JP
- Japan
- Prior art keywords
- sample
- sphingomyelinase
- fluorescence
- alkaline
- supernatant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000523 sample Substances 0.000 claims 10
- 102100036093 Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 Human genes 0.000 claims 8
- 101000876377 Homo sapiens Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 Proteins 0.000 claims 8
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 6
- DHCLVCXQIBBOPH-UHFFFAOYSA-N Glycerol 2-phosphate Chemical compound OCC(CO)OP(O)(O)=O DHCLVCXQIBBOPH-UHFFFAOYSA-N 0.000 claims 6
- 238000000034 method Methods 0.000 claims 6
- 239000006228 supernatant Substances 0.000 claims 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 4
- 108010061312 Sphingomyelin Phosphodiesterase Proteins 0.000 claims 4
- 102000011971 Sphingomyelin Phosphodiesterase Human genes 0.000 claims 4
- 239000007983 Tris buffer Substances 0.000 claims 4
- 239000012131 assay buffer Substances 0.000 claims 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 4
- PKYCWFICOKSIHZ-UHFFFAOYSA-N 1-(3,7-dihydroxyphenoxazin-10-yl)ethanone Chemical compound OC1=CC=C2N(C(=O)C)C3=CC=C(O)C=C3OC2=C1 PKYCWFICOKSIHZ-UHFFFAOYSA-N 0.000 claims 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 3
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 3
- 108010000659 Choline oxidase Proteins 0.000 claims 3
- 230000001580 bacterial effect Effects 0.000 claims 3
- 239000003833 bile salt Substances 0.000 claims 3
- 239000012472 biological sample Substances 0.000 claims 3
- 238000001514 detection method Methods 0.000 claims 3
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 claims 3
- 239000011539 homogenization buffer Substances 0.000 claims 3
- 239000011535 reaction buffer Substances 0.000 claims 3
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 claims 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims 2
- 102100024550 Sphingomyelin phosphodiesterase 2 Human genes 0.000 claims 2
- 239000012620 biological material Substances 0.000 claims 2
- 239000007795 chemical reaction product Substances 0.000 claims 2
- 238000001035 drying Methods 0.000 claims 2
- 230000005284 excitation Effects 0.000 claims 2
- 238000000338 in vitro Methods 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 108040005466 neutral sphingomyelin phosphodiesterase activity proteins Proteins 0.000 claims 2
- 239000011780 sodium chloride Substances 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 1
- 102000003992 Peroxidases Human genes 0.000 claims 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 1
- 229930006000 Sucrose Natural products 0.000 claims 1
- 244000195452 Wasabia japonica Species 0.000 claims 1
- 235000000760 Wasabia japonica Nutrition 0.000 claims 1
- 230000002378 acidificating effect Effects 0.000 claims 1
- 210000000941 bile Anatomy 0.000 claims 1
- 229940093761 bile salts Drugs 0.000 claims 1
- 239000012141 concentrate Substances 0.000 claims 1
- 238000010790 dilution Methods 0.000 claims 1
- 239000012895 dilution Substances 0.000 claims 1
- 210000003608 fece Anatomy 0.000 claims 1
- 239000004615 ingredient Substances 0.000 claims 1
- 238000005259 measurement Methods 0.000 claims 1
- 108040007629 peroxidase activity proteins Proteins 0.000 claims 1
- 239000005720 sucrose Substances 0.000 claims 1
- 239000013595 supernatant sample Substances 0.000 claims 1
- 239000000725 suspension Substances 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IE2001/1100 | 2001-12-21 | ||
| IE20011100A IE20011100A1 (en) | 2001-12-21 | 2001-12-21 | Analytical Method for Detecting Alkaline Sphingomyelinase and Kit for Use in Such Method |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003556548A Division JP2005512601A (ja) | 2001-12-21 | 2002-12-19 | アルカリ性スフィンゴミエリナーゼを検出するための分析方法およびかかる方法に使用するキット |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2009195239A JP2009195239A (ja) | 2009-09-03 |
| JP2009195239A5 true JP2009195239A5 (enExample) | 2009-10-15 |
| JP2009195239A6 JP2009195239A6 (ja) | 2009-12-24 |
| JP5009332B2 JP5009332B2 (ja) | 2012-08-22 |
Family
ID=11042874
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003556548A Pending JP2005512601A (ja) | 2001-12-21 | 2002-12-19 | アルカリ性スフィンゴミエリナーゼを検出するための分析方法およびかかる方法に使用するキット |
| JP2009104135A Expired - Fee Related JP5009332B2 (ja) | 2001-12-21 | 2009-04-22 | アルカリ性スフィンゴミエリナーゼを検出するための分析方法およびかかる方法に使用するキット |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003556548A Pending JP2005512601A (ja) | 2001-12-21 | 2002-12-19 | アルカリ性スフィンゴミエリナーゼを検出するための分析方法およびかかる方法に使用するキット |
Country Status (28)
| Country | Link |
|---|---|
| US (3) | US20050118152A1 (enExample) |
| EP (1) | EP1456405B1 (enExample) |
| JP (2) | JP2005512601A (enExample) |
| KR (1) | KR101002068B1 (enExample) |
| CN (1) | CN101126716A (enExample) |
| AR (1) | AR038037A1 (enExample) |
| AT (1) | ATE408707T1 (enExample) |
| AU (1) | AU2002367123B2 (enExample) |
| BR (1) | BRPI0215045B1 (enExample) |
| CA (1) | CA2469796C (enExample) |
| CY (1) | CY1108512T1 (enExample) |
| DE (1) | DE60228993D1 (enExample) |
| DK (1) | DK1456405T3 (enExample) |
| EG (1) | EG25804A (enExample) |
| ES (1) | ES2312666T3 (enExample) |
| HR (1) | HRP20040632B1 (enExample) |
| HU (1) | HU228812B1 (enExample) |
| IE (1) | IE20011100A1 (enExample) |
| IL (2) | IL162513A0 (enExample) |
| MX (1) | MXPA04005919A (enExample) |
| NO (1) | NO331806B1 (enExample) |
| NZ (1) | NZ533806A (enExample) |
| PL (1) | PL207876B1 (enExample) |
| PT (1) | PT1456405E (enExample) |
| RU (1) | RU2316002C2 (enExample) |
| SI (1) | SI1456405T1 (enExample) |
| WO (1) | WO2003056031A2 (enExample) |
| ZA (1) | ZA200405762B (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IE20011100A1 (en) | 2001-12-21 | 2003-07-09 | Vsl Pharma Ltd | Analytical Method for Detecting Alkaline Sphingomyelinase and Kit for Use in Such Method |
| PL1973561T3 (pl) * | 2005-12-15 | 2011-08-31 | Elanco Us Inc | Enzymy do zmniejszania stresu immunologicznego |
| CN103760348B (zh) * | 2014-02-11 | 2015-03-11 | 苏州博源医疗科技有限公司 | 一种甘胆酸免疫检测试剂及其制备和检测方法 |
| CN104614371A (zh) * | 2015-02-15 | 2015-05-13 | 史春龙 | 一种在便器中检测排泄物生化指标的方法及使用该方法的检测设备 |
| CN116106281A (zh) * | 2023-02-03 | 2023-05-12 | 大连医科大学 | 一种粪便样本中肠道菌脂肪酶活性的检测方法 |
| CN117630364B (zh) * | 2023-12-05 | 2024-07-30 | 临沂大学 | 一种双酶型免疫荧光系统检测痕量黄曲霉毒素b1的方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1311495B1 (it) * | 1999-06-09 | 2002-03-13 | Mendes S U R L | Composizione comprendente sfingomielinasi alcalina, utilizzabile qualeprodotto dietetico, integratore alimentare o medicamento. |
| US6265179B1 (en) * | 2000-02-01 | 2001-07-24 | Molecular Probes, Inc. | Detection of phosphate using coupled enzymatic reactions |
| IE20011100A1 (en) | 2001-12-21 | 2003-07-09 | Vsl Pharma Ltd | Analytical Method for Detecting Alkaline Sphingomyelinase and Kit for Use in Such Method |
-
2001
- 2001-12-21 IE IE20011100A patent/IE20011100A1/en not_active IP Right Cessation
-
2002
- 2002-12-19 DE DE60228993T patent/DE60228993D1/de not_active Expired - Lifetime
- 2002-12-19 RU RU2004122426/15A patent/RU2316002C2/ru not_active IP Right Cessation
- 2002-12-19 AT AT02805875T patent/ATE408707T1/de active
- 2002-12-19 EP EP02805875A patent/EP1456405B1/en not_active Expired - Lifetime
- 2002-12-19 HU HU0402542A patent/HU228812B1/hu not_active IP Right Cessation
- 2002-12-19 IL IL16251302A patent/IL162513A0/xx unknown
- 2002-12-19 AU AU2002367123A patent/AU2002367123B2/en not_active Ceased
- 2002-12-19 DK DK02805875T patent/DK1456405T3/da active
- 2002-12-19 PT PT2805875T patent/PT1456405E/pt unknown
- 2002-12-19 BR BRPI0215045A patent/BRPI0215045B1/pt not_active IP Right Cessation
- 2002-12-19 CN CNA200710147169XA patent/CN101126716A/zh active Pending
- 2002-12-19 WO PCT/IT2002/000811 patent/WO2003056031A2/en not_active Ceased
- 2002-12-19 KR KR1020047008886A patent/KR101002068B1/ko not_active Expired - Fee Related
- 2002-12-19 NZ NZ533806A patent/NZ533806A/en not_active IP Right Cessation
- 2002-12-19 MX MXPA04005919A patent/MXPA04005919A/es active IP Right Grant
- 2002-12-19 AR ARP020104993A patent/AR038037A1/es active IP Right Grant
- 2002-12-19 SI SI200230740T patent/SI1456405T1/sl unknown
- 2002-12-19 CA CA2469796A patent/CA2469796C/en not_active Expired - Fee Related
- 2002-12-19 ES ES02805875T patent/ES2312666T3/es not_active Expired - Lifetime
- 2002-12-19 US US10/499,336 patent/US20050118152A1/en not_active Abandoned
- 2002-12-19 HR HRP20040632AA patent/HRP20040632B1/hr not_active IP Right Cessation
- 2002-12-19 JP JP2003556548A patent/JP2005512601A/ja active Pending
- 2002-12-19 PL PL374085A patent/PL207876B1/pl unknown
- 2002-12-21 EG EG2002121379A patent/EG25804A/xx active
-
2004
- 2004-06-14 IL IL162513A patent/IL162513A/en not_active IP Right Cessation
- 2004-07-13 NO NO20042992A patent/NO331806B1/no not_active IP Right Cessation
- 2004-07-20 ZA ZA200405762A patent/ZA200405762B/en unknown
-
2006
- 2006-02-23 US US11/359,619 patent/US7211410B2/en not_active Expired - Fee Related
-
2007
- 2007-03-01 US US11/712,417 patent/US7323317B2/en not_active Expired - Fee Related
-
2008
- 2008-11-13 CY CY20081101308T patent/CY1108512T1/el unknown
-
2009
- 2009-04-22 JP JP2009104135A patent/JP5009332B2/ja not_active Expired - Fee Related
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