JP2009183146A - 新規微生物、当該新規微生物を用いたドデカヒドロ−3a,6,6,9a−テトラメチルナフト[2,1−b]フラン中間体の製造方法 - Google Patents
新規微生物、当該新規微生物を用いたドデカヒドロ−3a,6,6,9a−テトラメチルナフト[2,1−b]フラン中間体の製造方法 Download PDFInfo
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- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract
【解決手段】目的の特性を有する微生物を単離、同定すべく鋭意検討した結果、従来公知の微生物には分類されない、目的の特性を有する複数の新規微生物を単離、同定した。本発明に係る新規微生物は、Rhodococcus属に属し、スクラレオールを基質として、ドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン中間体を生成する能力を有する。当該能力を有するRhodococcus属に属する微生物は新規な知見であり、ドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン及びその中間体の製造に有用な微生物となりうる。
【選択図】なし
Description
本発明に係る新規微生物は、Rhodococcus属に属し、スクラレオールを基質としてドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン合成過程における中間体(ドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン中間体)を生成する微生物である。ここで、ドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン中間体とは、デカヒドロ2-ヒドロキシ-2,5,5,8a-テトラメチルナフタレン-1-エタノール及びスクラレオリド(デカヒドロ3a,6,6,9a-テトラメチルナフト[2,1-b]フラン-2(1H)-オン)を意味する。特に、本発明に係る新規微生物は、これら中間体のなかでもデカヒドロ2-ヒドロキシ-2,5,5,8a-テトラメチルナフタレン-1-エタノールを生成する能力を有している。なお、本発明に係る新規微生物は、デカヒドロ2-ヒドロキシ-2,5,5,8a-テトラメチルナフタレン-1-エタノール生成能を有していれば、これに加えてスクラレオリドを生成する能力を更に有していても良い。
上述した本発明に係る新規微生物を使用して、ドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン中間体を製造することができる。製造されたドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン中間体は、付加価値の高い、残香性の高い香料であるドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フランを製造する際の原料として使用することができる。
以下のようにして、三重県鈴鹿市及び東京都江戸川区の土壌より新規微生物の単離を行った。
実施例1で単離したKSM-JL3867及びKSM-JL4876の菌学的性質を特定するとともにrDNAを解析することで、これらの菌株の分類を試みた。
本実施例では、Rhodococcus sp. KSM-JL3867及びRhodococcus sp. KSM-JL4876のドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン中間体の生産性を検討した。本菌を先ず、0.2% 酵母エキス、0.2% 硝酸アンモニウム、0.1% リン酸1カリウム及び0.05% 硫酸マグネシウム・7水和物を含有する液体培地に接種し、25℃で1日間培養し種菌とした。次に、0.2% 酵母エキス、0.2% 硝酸アンモニウム、0.1% リン酸1カリウム、0.05% 硫酸マグネシウム・7水和物、1.0% スクラレオール(別滅菌)及び0.5% ツイーン80(別滅菌)を含有する培地に種菌を1%植菌し、25℃にて1週間培養した。培養液は実施例1の手法にて抽出及びGC分析を行い、ドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン中間体の生産量を求めた。結果を表3に示す。なお、表3に示した数値の単位は“g/L”である
Claims (8)
- Rhodococcus属に属し、スクラレオール(Sclareol)を基質として、ドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン合成過程における中間体を生成する能力を有する微生物。
- 配列番号1〜2のいずれかに示す塩基配列に対して95%以上の相同性を有する塩基配列からなる16S rDNAを有することを特徴とする請求項1記載の微生物。
- 受託番号FERM P-21432もしくはFERM P-21433で特定されるRhodococcus属と同一の種に属する微生物であることを特徴とする請求項1乃至3いずれか一項記載の微生物。
- 受託番号FERM P-21432もしくはFERM P-21433で特定されるRhodococcus属であることを特徴とする請求項1乃至3いずれか一項記載の微生物。
- 上記中間体は、デカヒドロ2-ヒドロキシ-2,5,5,8a-テトラメチルナフタレン-1-エタノールであることを特徴とする請求項1乃至5いずれか一項記載の微生物。
- スクラレオール(Sclareol)を含有する培地で請求項1乃至6いずれか一項記載の微生物を培養し、スクラレオールを基質としてドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン合成過程における中間体を生成する、ドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン中間体の製造方法。
- 上記中間体として、デカヒドロ2-ヒドロキシ-2,5,5,8a-テトラメチルナフタレン-1-エタノールを生成する請求項7記載のドデカヒドロ-3a,6,6,9a-テトラメチルナフト[2,1-b]フラン中間体の製造方法。
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