JP2009178099A - Malt alcoholic beverage and method for producing the same - Google Patents

Malt alcoholic beverage and method for producing the same Download PDF

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JP2009178099A
JP2009178099A JP2008020664A JP2008020664A JP2009178099A JP 2009178099 A JP2009178099 A JP 2009178099A JP 2008020664 A JP2008020664 A JP 2008020664A JP 2008020664 A JP2008020664 A JP 2008020664A JP 2009178099 A JP2009178099 A JP 2009178099A
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alcoholic beverage
malt
mulberry leaves
malt alcoholic
added
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Koichi Suzuki
幸一 鈴木
Shinzo Noda
信三 野田
Koki Sato
晄僖 佐藤
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SEKINOICHI SHUZO KK
TOYOTAMA KENKO SHOKUHIN KK
Iwate University
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SEKINOICHI SHUZO KK
TOYOTAMA KENKO SHOKUHIN KK
Iwate University
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Abstract

<P>PROBLEM TO BE SOLVED: To provide a malt alcoholic beverage that has a flavor and a strong taste of body, is easily drinkable and has an inhibitory effect on increase in blood sugar level after ingestion and a method for producing the same. <P>SOLUTION: The method for producing the malt alcoholic beverage containing a mulberry leaf-derived DNJ (deoxynojirimycin) comprises adding mulberry leaves in any process up to an aging process in a brewing course including at least a saccharification process, a boiling process, a fermentation process, an aging process and a filtration process and/or adding a mulberry leaf fermented product in any process from the saccharification process to a process just before the filtration process. The malt alcoholic beverage is produced by the method. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

本発明は、桑葉を用いた麦芽アルコール飲料及びその製造方法に関する。   The present invention relates to a malt alcoholic beverage using mulberry leaves and a method for producing the same.

デオキシノジリマイシン(DNJ)は、ブドウ糖の1つの酸素が窒素に置き換わった構造をしたアザ糖類(窒素を環内の異節原子としてもつ糖類)の一種であり、糖質分解酵素であるα−グルコシダーゼの阻害剤として働くとともに、ブドウ糖の腸管からの吸収を抑制することにより、食後の血糖値の上昇を抑制することが報告されている。DNJは桑葉に比較的豊富に含まれているため、桑葉を利用した茶や健康食品、サプリメント等が開発されている。   Deoxynojirimycin (DNJ) is a kind of azasaccharide (a saccharide having nitrogen as a heteroatom in a ring) having a structure in which one oxygen of glucose is replaced by nitrogen, and α-glucosidase which is a carbohydrase It has been reported that it suppresses an increase in blood glucose level after a meal by suppressing absorption of glucose from the intestinal tract while acting as an inhibitor of glucose. Since DNJ is relatively abundant in mulberry leaves, teas, health foods, supplements and the like using mulberry leaves have been developed.

アルコール飲料の原料や製法を工夫することにより付加価値の高いアルコール飲料を開発することは一般に行われている。桑をアルコール飲料に利用する試みとしては、エタノール抽出した桑葉エキスを醸造済みのビールに添加したもの(特許文献1)がある。また、味をまろやかにする目的でアントシアニンが多量に含まれているマルベリー(桑の実)を隠し味として赤ワインに入れたマルベリーワインが、ベトナムなどで製造されている。特許文献2には、エステル香の高いビール製造方法が開示されている。   It is common practice to develop high-value-added alcoholic beverages by devising alcoholic beverage ingredients and production methods. As an attempt to use mulberry for alcoholic beverages, there is one in which mulberry leaf extract extracted with ethanol is added to brewed beer (Patent Document 1). Mulberry wine is also produced in Vietnam, etc., in which red mulberry, which contains a large amount of anthocyanins as a hidden taste, is added to red wine for the purpose of mildness. Patent Document 2 discloses a method for producing beer with a high ester flavor.

特開平2001−136953号公報Japanese Patent Laid-Open No. 2001-136953 特開平06−030756号公報Japanese Patent Laid-Open No. 06-030756

本発明は、旨みとコク味が強く、飲みやすい麦芽アルコール飲料であって、摂取後の血糖値上昇抑制効果を有する麦芽アルコール飲料及びその製造方法を提供することを目的とする。   An object of the present invention is to provide a malt alcoholic beverage that has a strong taste and rich taste and is easy to drink, and that has an effect of suppressing an increase in blood glucose level after ingestion, and a method for producing the same.

本発明によれば、
〔1〕 少なくとも糖化工程、煮沸工程、発酵工程、熟成工程及びろ過工程を含む醸造過程における、熟成工程までのいずれかの工程において桑葉を、及び/又は糖化工程からろ過工程直前までのいずれかの工程において桑葉発酵物を、添加することを特徴とする、桑葉由来DNJを含有する麦芽アルコール飲料の製造方法;
〔2〕 醸造過程が、原料供給工程を含み、原料供給工程から熟成工程のいずれかにおいて桑葉を添加する、前記〔1〕記載の製造方法;
〔3〕 煮沸工程から熟成工程のいずれかにおいて桑葉を添加する、前記〔1〕記載の製造方法;
〔4〕 少なくとも糖化工程、煮沸工程、発酵工程、熟成工程及びろ過工程を含む醸造過程における、熟成工程までのいずれかの工程において桑葉を、及び/又は糖化工程からろ過工程直前までのいずれかの工程において桑葉発酵物を、添加したことにより、桑葉由来DNJを含有する、麦芽アルコール飲料;
〔5〕 醸造過程が、原料供給工程を含み、原料供給工程から熟成工程のいずれかにおいて桑葉を添加した、前記〔4〕記載の麦芽アルコール飲料;及び
〔6〕 煮沸工程から熟成工程のいずれかにおいて桑葉を添加した、前記〔4〕記載の麦芽アルコール飲料、
が提供される。
According to the present invention,
[1] Any of the brewing process including at least a saccharification process, a boiling process, a fermentation process, an aging process and a filtration process, and / or any of the mulberry leaves from the saccharification process to just before the filtration process. A method for producing a malt alcoholic beverage containing mulberry leaf-derived DNJ, wherein a fermented mulberry leaf is added in the step of;
[2] The production method according to [1], wherein the brewing process includes a raw material supply step, and mulberry leaves are added in any of the raw material supply step to the aging step;
[3] The production method according to [1], wherein mulberry leaves are added in any of the boiling step to the aging step;
[4] Any of the brewing process including at least a saccharification process, a boiling process, a fermentation process, an aging process and a filtration process, and / or any one of the processes from the saccharification process to just before the filtration process. A malt alcoholic beverage containing mulberry leaf-derived DNJ by adding a fermented mulberry leaf in the process of
[5] The malting alcoholic beverage according to [4] above, wherein the brewing process includes a raw material supply process, and mulberry leaves are added in any of the raw material supply process to the aging process; and [6] any of the boiling process to the aging process The malt alcoholic beverage according to [4], wherein mulberry leaves are added in
Is provided.

本発明によれば、旨み、コクがあり、飲みやすい上、摂取後の血糖値の上昇が抑制される麦芽アルコール飲料及びその製造方法が提供される。本発明の麦芽アルコール飲料は、醸造過程で、桑葉そのもの又は桑葉の発酵物を加えることにより、桑葉に含まれる遊離アミノ酸及びミネラルが飲料に移行するので、旨みとコク味が豊富である。さらに、桑葉の機能性成分DNJの作用により、麦芽・ホップ・副原料等由来の糖や副食に含まれる糖による食後血糖値の上昇が抑制されるため、健康維持に適する美味しい麦芽アルコール飲料を創製できる。また、本発明の麦芽アルコール飲料は、桑葉又は桑葉の発酵物を加えること以外は一般的なビール、発泡酒等の製造方法と同様の工程で製造することができるので、製造工程が簡便で、特別な設備・器具等を必要とせず、製造上も有利である。   ADVANTAGE OF THE INVENTION According to this invention, the malt alcoholic beverage which has taste and richness, is easy to drink, and the raise of the blood glucose level after ingestion is suppressed, and its manufacturing method are provided. The malt alcoholic beverage of the present invention is rich in umami and richness because the free amino acids and minerals contained in mulberry leaves are transferred to the beverage by adding mulberry leaves themselves or fermented mulberry leaves during the brewing process. . Furthermore, the action of the functional component DNJ of mulberry leaves suppresses the increase in postprandial blood glucose level due to sugars derived from malt, hops, auxiliary ingredients, etc. and sugars contained in side meals. Can be created. In addition, the malt alcoholic beverage of the present invention can be manufactured in the same process as a general method for manufacturing beer, happoshu, etc., except for adding mulberry leaves or fermented mulberry leaves, so the manufacturing process is simple. Thus, it does not require any special equipment / equipment and is advantageous in terms of manufacturing.

本発明に関して、麦芽アルコール飲料とは、麦芽を原料として得られるアルコール飲料であり、麦芽の使用比率や副原料等の使用原料などによって、ビール、発泡酒、雑酒又はその他の発泡性酒類等に分類されるものを含む。   In the context of the present invention, a malt alcoholic beverage is an alcoholic beverage obtained from malt as a raw material, and depending on the raw materials used such as malt use ratio and auxiliary materials, beer, sparkling wine, miscellaneous sake or other sparkling alcoholic beverages, etc. Includes what is classified.

本発明の麦芽アルコール飲料は、仕込(糖化・煮沸)、発酵、貯酒(熟成)及びろ過を含む、ビール、発泡酒等の麦芽アルコール飲料の一般的な製造方法において、原料供給工程〜熟成工程(好ましくは、煮沸工程〜熟成工程)のいずれかにおいて桑葉を、及び/又は糖化工程〜ろ過工程直前までのいずれかにおいて桑葉発酵物を、原料として加えて製造されることを特徴とする。   The malt alcoholic beverage of the present invention is a raw material supply step to an aging step in a general production method of malt alcoholic beverages such as beer and happoshu, including preparation (saccharification / boiling), fermentation, storage (aging) and filtration. Preferably, it is produced by adding mulberry leaves in any of boiling step to aging step) and / or fermented mulberry leaves as raw materials in any of the saccharification step to immediately before the filtration step.

一般に、仕込工程に使用する前に、原料の麦芽を荒く砕く処理が行われる(製麦工程)。製麦工程で得られた麦芽は、麦芽のみで用いてもよいが、副原料と共に用いてもよい。本発明において使用されうる副原料としては、一般的な副原料、例えば、米、トウモロコシ、コウリャン、馬鈴薯、デンプン、糖類等;コムギ、ライ麦、サツマイモ、サトイモ、各種のデンプン質原料又は糖類、果汁等が挙げられるが、これらに限らない。   Generally, before using for a preparation process, the raw malt is roughly crushed (a malting process). The malt obtained in the malting process may be used only in the malt, but may be used together with the auxiliary materials. Examples of auxiliary materials that can be used in the present invention include general auxiliary materials such as rice, corn, cucumber, potato, starch, saccharides, etc .; wheat, rye, sweet potato, taro, various starchy materials or saccharides, fruit juice, etc. However, it is not limited to these.

仕込工程においては、まず、麦芽又は麦芽及び副原料を糖化釜に入れ、温水と混ぜて保温することにより麦芽中のデンプンを糖分に変化させる(糖化工程)。糖化条件は特に制限されないが、具体的には、例えば、重量比で麦芽1に対して1〜5倍(好ましくは2.5〜3.0倍)の水を入れ、0.5〜2時間の間に段階的に60〜80℃まで加温するか、前記のような所定の温度で前記同様の時間維持することができる。   In the preparation process, first, malt or malt and an auxiliary material are put into a saccharification pot, mixed with warm water and kept warm to change the starch in the malt into sugar (saccharification process). The saccharification conditions are not particularly limited. Specifically, for example, 1 to 5 times (preferably 2.5 to 3.0 times) water is added to malt 1 in a weight ratio, and 0.5 to 2 hours. The temperature can be gradually increased to 60 to 80 ° C. during this period, or can be maintained at the predetermined temperature as described above for the same time.

糖化終了後、ろ過して得た精製された麦汁を煮沸釜(ケトル)に移し、ホップを加えて煮沸する(煮沸工程)。煮沸工程では、例えば、30Lの麦汁に対して100gのホップを添加し、1〜2時間、激しく煮沸することができる。   After completion of saccharification, the purified wort obtained by filtration is transferred to a boiling kettle, and hops are added to boil (boiling step). In the boiling step, for example, 100 g of hops can be added to 30 L of wort and boiled vigorously for 1 to 2 hours.

発酵工程においては、煮沸が終わった麦汁を、例えば19〜22℃程度(好ましくは20℃以下)まで冷却して、発酵タンクに移し、酵母を加えて温度管理下(例えば約20℃以下)で1〜7日間、好ましくは3〜4日間発酵させる。ここで添加する酵母は、アルコール発酵を行ういわゆるビール酵母であればよく、その種類及び量は当業者が適宜選択することができる。発酵工程中に、酵母により麦汁の中の糖分がアルコールと炭酸ガスとに分解される。発酵終了と同時に徐々に発酵液の温度を下げる。例えば約5℃になったら、沈殿している酵母を取り除いてもよい。   In the fermentation process, the boiled wort is cooled to, for example, about 19 to 22 ° C. (preferably 20 ° C. or less), transferred to a fermentation tank, and yeast is added under temperature control (for example, about 20 ° C. or less). For 1 to 7 days, preferably 3 to 4 days. The yeast added here may be so-called brewer's yeast that performs alcohol fermentation, and the type and amount thereof can be appropriately selected by those skilled in the art. During the fermentation process, the sugar in the wort is broken down into alcohol and carbon dioxide by the yeast. Gradually lower the temperature of the fermentation broth at the end of fermentation. For example, when the temperature reaches about 5 ° C., the precipitated yeast may be removed.

発酵終了後、発酵液の上澄みだけを熟成タンクに移し、貯蔵温度で熟成させる貯酒(熟成)工程を行う。熟成は、例えば−1〜7℃(好ましくは約4℃)で約1〜3週間、行うことができる。   After the fermentation is completed, only the supernatant of the fermentation broth is transferred to the aging tank, and a brewing (aging) process is performed in which the aging is performed at the storage temperature. The aging can be performed, for example, at -1 to 7 ° C (preferably about 4 ° C) for about 1 to 3 weeks.

その後、場合によりろ過工程・殺菌工程を行って、瓶詰め又は樽詰め等の製品化作業を行う。ろ過は、一般的には、公知のろ過助剤(珪藻土、シリカゲルなど)を用いて行うことができる。   Then, if necessary, a filtration process and a sterilization process are performed, and productization work such as bottling or barreling is performed. Filtration can generally be performed using a known filter aid (diatomaceous earth, silica gel, etc.).

本発明の麦芽アルコール飲料の製造においては、上記のような工程を含む製造過程において、原料供給工程(即ち、仕込前の準備工程、例えば製麦工程)〜熟成工程のいずれかにおいて桑の葉を、及び/又は糖化工程〜ろ過工程直前までのいずれかにおいて桑葉発酵物を、添加する。ここでいう桑は、クワ科クワ属植物であればよく、ヤマグワ(Morus bombycis)、カラヤマグワ(M. alba)、クロミグワ(M. nigara)、アカミグワ(M. rubral)、ロソウ(M. latifolia)やこれらの交配種、変種、栽培種等であることができる。また、葉は、成長した葉でも芽でもよく、新鮮なものでも乾燥物などでもよい。形状は、例えば乾燥物の粉末等の形態であることができるが、特に制限はない。添加量は、所望に応じて適宜調節することができるが、麦汁に対する重量比で0.01〜40%程度で十分な効果を期待できる。好ましくは3〜5%程度である。   In the production of the malt alcoholic beverage of the present invention, in the production process including the steps as described above, the mulberry leaves are used in any of the raw material supply step (ie, the preparatory step before preparation, for example, the malting step) to the ripening step. And / or fermented mulberry leaves in any of the saccharification step to just before the filtration step. The mulberry here may be a mulberry plant, such as Yamasuwa (Morus bombycis), Karayamaguwa (M. alba), Kuromiguwa (M. nigara), Akamiguwa (M. rubral), Rosou (M. latifolia) and These can be hybrids, varieties, cultivated species, and the like. The leaves may be grown leaves or buds, fresh or dried. The shape can be, for example, a dry powder or the like, but is not particularly limited. The amount added can be appropriately adjusted as desired, but a sufficient effect can be expected at a weight ratio of about 0.01 to 40% with respect to wort. Preferably it is about 3 to 5%.

DNJは、水溶性の成分であるため、桑葉を水又は水溶液(麦汁又は発酵液など)に添加することによりこれらの液中に浸出・移行する。そのため、桑葉を添加する場合は、原料供給工程(仕込前の準備工程)において、仕込み水又は麦芽などのいずれかの原料に添加しておいてもよく、また、遅くとも熟成工程中に添加すればよい。しかし、一般的には煮沸工程〜熟成工程が好ましく、発酵工程の終了前までが特に好ましい。これは、桑葉を発酵させることにより、桑葉の細胞が壊れてDNJなどの水溶性成分が抽出されやすくなると共に、タンパク分解作用によってアミノ酸などの旨み成分もより増加すると考えられるためである。なお、従来、DNJは、糖分吸収を阻害するので発酵しないと考えられていた。しかし、本発明においては、発酵工程時に桑の葉を共存させて発酵させるとCOガスが発生し、発酵していることが確認されている。 Since DNJ is a water-soluble component, mulberry leaves are leached and transferred into these liquids by adding them to water or an aqueous solution (such as wort or fermentation broth). Therefore, when adding mulberry leaves, it may be added to any raw material such as feed water or malt in the raw material supply step (preparation step before charging), or added at the latest during the ripening step. That's fine. However, in general, a boiling step to an aging step are preferable, and until the end of the fermentation step is particularly preferable. This is because it is considered that fermenting mulberry leaves breaks mulberry leaf cells and facilitates extraction of water-soluble components such as DNJ and also increases umami components such as amino acids due to proteolytic action. Conventionally, DNJ has been thought not to ferment because it inhibits sugar absorption. However, in the present invention, it has been confirmed that CO 2 gas is generated and fermented when mulberry leaves coexist in the fermentation process.

桑葉をそのまま添加する代わりに、あるいは桑葉の添加と併用して、桑葉の発酵物を添加することもできる。桑葉の発酵物は、例えば、桑の葉に酵母やアミラーゼ酵素等を添加して発酵させたものとして調製することができる。桑葉の発酵物は、糖化工程〜ろ過工程の前であればどの工程で添加してもよい。   Instead of adding mulberry leaves as they are, or in combination with addition of mulberry leaves, fermented mulberry leaves can be added. The fermented mulberry leaf can be prepared, for example, by fermenting mulberry leaves with yeast or amylase enzyme. The fermented mulberry leaves may be added in any step as long as it is before the saccharification step to the filtration step.

本発明の製造方法は、一般に上述の工程を含むが、それらに加えて任意の工程を含んでいることができる。   The production method of the present invention generally includes the above-described steps, but may include any step in addition to them.

実施例1:桑葉を発酵に用いた麦芽アルコール飲料の製造
麦芽(ピルスナモルト:小麦麦芽=10:1)15kgを、粉砕機で粉砕した。
Example 1 Production of Malt Alcohol Beverage Using Mulberry Leaves for Fermentation 15 kg of malt (Pilsnamalt: wheat malt = 10: 1) was pulverized with a pulverizer.

仕込みタンク(通称、マッシュロイター)に、粉砕した麦芽と麦芽重量1に対して2.7倍重量の仕込み水とを入れた。60℃まで加温し、20分放置した。その後、67.5℃に加温し、60分放置して、75℃に加温して糖化を終了した。   A charged tank (commonly known as Mash Reuters) was charged with crushed malt and 2.7 times the weight of charged water with respect to 1 malt weight. Heated to 60 ° C. and left for 20 minutes. Thereafter, the mixture was heated to 67.5 ° C., left for 60 minutes, and heated to 75 ° C. to complete saccharification.

麦汁を、マッシュロイターでろ過し、精製した後にケトルに移した。   The wort was filtered through a mash reuter and purified, and then transferred to a kettle.

ケトルで60分、100℃で煮沸した(初期比重 1.060)。ここで、沸騰後30分後に、ホップ100g、及び桑の葉粉末280g(麦芽及び水の全体量に対して0.7%)を投入した。煮沸終了後の比重は1.075であった。   It was boiled in a kettle for 60 minutes at 100 ° C. (initial specific gravity 1.060). Here, 30 minutes after boiling, 100 g of hops and 280 g of mulberry leaf powder (0.7% based on the total amount of malt and water) were added. The specific gravity after boiling was 1.075.

この麦汁を、分離タンク(ワールプール)で15分処理した後、45分放置した。麦汁を、熱交換器を通し、麦汁温度を18℃に下げながら、発酵タンクに移した。   This wort was treated in a separation tank (Whirpool) for 15 minutes and then left for 45 minutes. The wort was transferred to the fermentation tank through a heat exchanger while lowering the wort temperature to 18 ° C.

ビール酵母2.8kg(麦芽及び水の全体量に対して5%)を投入し、約20℃までの温度で3日間発酵させた。発酵終了と同時に、1日1℃ずつ麦汁温度を下げ、5℃になったところで沈殿している酵母を取り除いた。   2.8 kg of brewer's yeast (5% based on the total amount of malt and water) was added and fermented at a temperature up to about 20 ° C. for 3 days. Simultaneously with the end of the fermentation, the wort temperature was lowered by 1 ° C. per day, and the precipitated yeast was removed when the temperature reached 5 ° C.

熟成タンクに移し、4℃で2週間熟成させた後、瓶詰めを行った。   After transferring to an aging tank and aging at 4 ° C. for 2 weeks, bottling was performed.

完成した麦芽アルコール飲料を試料として、成分分析を行った結果を表1及び表2に示す。   Tables 1 and 2 show the results of component analysis using the finished malt alcoholic beverage as a sample.

Figure 2009178099
Figure 2009178099

遊離アミノ酸の分析試験結果:アミノ酸自動分析法によって測定した。   Analysis results of free amino acids: Measured by amino acid automatic analysis.

Figure 2009178099
Figure 2009178099

ミネラルの分析結果:原子吸光光度法によって測定した。   Mineral analysis results: Measured by atomic absorption spectrophotometry.

表2のデータからは、本発明の麦芽アルコール飲料には桑葉に特徴的なアミノ酸などが豊富に含有され、うま味成分となっていることがわかる。   From the data in Table 2, it can be seen that the malt alcoholic beverage of the present invention is rich in amino acids characteristic of mulberry leaves and is an umami component.

試験例1:血糖値上昇抑制効果
以下の方法により、実施例1と同様にして製造された本発明の麦芽アルコール飲料による血糖値上昇抑制効果を従来技術によるビールと比較した。
Test Example 1: Blood glucose level increase inhibitory effect The blood glucose level increase inhibitory effect of the malt alcoholic beverage of the present invention produced in the same manner as in Example 1 was compared with beer according to the prior art by the following method.

試験区には、実施例1と同様にして製造された麦芽アルコール飲料(副原料として桑葉粉末5%添加、酒税法上は発泡酒に分類される;アルコール度約6〜8%)、対照区には世嬉の一酒造株式会社より提供された市販品ビール「インディアペールエール」(桑葉無添加;アルコール度約6%)を使用した。   In the test group, a malt alcoholic beverage produced in the same manner as in Example 1 (added with 5% mulberry leaf powder as an auxiliary material, classified under the liquor tax law as Happoshu; alcohol content of about 6-8%), control A commercial beer “India Pale Ale” (no mulberry leaves added; alcohol content of about 6%) provided by Seirei Ichizo Brewery Co., Ltd. was used in the ward.

健常者10名(男性5名、女性5名;平均年齢38.1歳)の被験者に、試験当日の昼食は午後12時〜13時の間に摂り、それ以降は何も摂らないよう要請し、16時より試験を実施した。昼食は、全員に同じ弁当(676kcal)を支給した。   Ten healthy subjects (5 males, 5 females; average age 38.1 years old) were asked to eat lunch on the day of the study between 12:00 pm and 13:00 pm, and then take nothing later. Tests were conducted from time to time. For lunch, the same lunch (676 kcal) was paid to all.

試験開始時(16時)に、330mlの試験品ビールを、おつまみ(亀田製菓、「柿の種」一袋:209kcal)を食べながら飲用させた。試験品の摂取直前、及び摂取後は15分おきに1時間まで及びそれ以降は30分おきに2時間まで、指先から血液を採集し、簡易グルコース測定機((株)アークレイファクトリー社製)により、血糖値を測定した。   At the start of the test (16:00), 330 ml of the test product beer was drunk while eating snacks (Kameda Seika, “Sakaki no Tane” bag: 209 kcal). Blood is collected from the fingertip immediately before taking the test product and every 15 minutes up to 1 hour after taking the test product and then every 30 minutes up to 2 hours thereafter, using a simple glucose meter (manufactured by Arkray Factory Co., Ltd.). The blood glucose level was measured.

結果を図1及び2に示す。対照のビール(桑葉無添加)を摂取すると、食後血糖値は30分まで急激に上昇し、それ以降徐々に低下した。これに対し、本発明の麦芽アルコール飲料を摂取すると、血糖値の上昇は有意に抑制された(図1)。本発明の麦芽アルコール飲料について、カロリーの相対吸収率を血糖上昇カーブの下方面積から計算すると、対照のビールに対し40.6%の抑制率であった(図2)。   The results are shown in FIGS. When the control beer (with no mulberry leaves) was ingested, the postprandial blood glucose level increased rapidly to 30 minutes and then gradually decreased. In contrast, when the malt alcoholic beverage of the present invention was ingested, the increase in blood glucose level was significantly suppressed (FIG. 1). When the relative absorption rate of calories was calculated from the lower area of the blood sugar increase curve for the malt alcoholic beverage of the present invention, it was 40.6% of the control beer (FIG. 2).

なお、有意差検定はt−検定法にしたがった。   In addition, the significant difference test followed the t-test method.

試験例2:官能評価
試験は、健常な男女10名(平均年齢34.9歳)に対しておこなった。各評価者は、もちを少量ずつ食べながら、評価結果を官能検査用紙に5段階(非常に良い=5点、良い=4点、普通=3点、悪い=2点、非常に悪い=1点)で記入し、さらにコメントを自由表記した。
Test Example 2: Sensory evaluation The test was conducted on 10 healthy men and women (average age: 34.9 years). Each evaluator eats a small amount of rice cake and puts the evaluation result on a sensory test paper in 5 stages (very good = 5 points, good = 4 points, normal = 3 points, bad = 2 points, very bad = 1 point) ), And the comments are written freely.

結果を表3及び図3に示す。対照のビールについては、苦味が強く、好みが分かれる傾向が見られたが、本発明の麦芽アルコール飲料については、対照と比べて苦味が抑えられており、まろやかで飲みやすい、甘さ・旨みがほど良い、色も香りも非常に良い、といった感想が多く、全体的に高評価を得た。   The results are shown in Table 3 and FIG. For the control beer, the bitterness was strong and there was a tendency to separate preferences, but for the malt alcoholic beverage of the present invention, the bitterness was suppressed compared to the control, and it was mellow and easy to drink, sweetness and taste There were many impressions that it was good and the color and fragrance were very good, and it was highly evaluated overall.

Figure 2009178099
Figure 2009178099

図1は、本発明の麦芽アルコール飲料(「桑葉粉末」と表示;以下同じ)の血糖上昇抑制効果を表す図である。FIG. 1 is a diagram showing the blood glucose elevation inhibitory effect of the malt alcoholic beverage of the present invention (indicated as “mulberry leaf powder”; hereinafter the same). 図2は、本発明の麦芽アルコール飲料のカロリー吸収抑制効果を表す図である。FIG. 2 is a diagram showing the calorie absorption suppression effect of the malt alcoholic beverage of the present invention. 図3は、本発明の麦芽アルコール飲料の官能検査による評価を表す図である。FIG. 3 is a diagram showing evaluation by sensory test of the malt alcoholic beverage of the present invention.

Claims (6)

少なくとも糖化工程、煮沸工程、発酵工程、熟成工程及びろ過工程を含む醸造過程における、熟成工程までのいずれかの工程において桑葉を、及び/又は糖化工程からろ過工程直前までのいずれかの工程において桑葉発酵物を、添加することを特徴とする、桑葉由来DNJを含有する麦芽アルコール飲料の製造方法。   In brewing process including at least saccharification process, boiling process, fermentation process, ripening process and filtration process, mulberry leaves in any process from ripening process and / or in any process from saccharification process to just before filtration process A method for producing a malt alcoholic beverage containing mulberry leaf-derived DNJ, wherein a fermented mulberry leaf is added. 醸造過程が、原料供給工程を含み、原料供給工程から熟成工程のいずれかにおいて桑葉を添加する、請求項1記載の製造方法。   The manufacturing method according to claim 1, wherein the brewing process includes a raw material supply process, and mulberry leaves are added in any of the raw material supply process to the aging process. 煮沸工程から熟成工程のいずれかにおいて桑葉を添加する、請求項1記載の製造方法。   The production method according to claim 1, wherein mulberry leaves are added in any of the boiling step to the aging step. 少なくとも糖化工程、煮沸工程、発酵工程、熟成工程及びろ過工程を含む醸造過程における、熟成工程までのいずれかの工程において桑葉を、及び/又は糖化工程からろ過工程直前までのいずれかの工程において桑葉発酵物を、添加したことにより、桑葉由来DNJを含有する、麦芽アルコール飲料。   In brewing process including at least saccharification process, boiling process, fermentation process, ripening process and filtration process, mulberry leaves in any process from ripening process and / or in any process from saccharification process to just before filtration process A malt alcoholic beverage containing mulberry leaf-derived DNJ by adding a mulberry leaf fermented product. 醸造過程が、原料供給工程を含み、原料供給工程から熟成工程のいずれかにおいて桑葉を添加した、請求項4記載の麦芽アルコール飲料。   The malt alcoholic beverage according to claim 4, wherein the brewing process includes a raw material supply step, and mulberry leaves are added in any of the raw material supply step to the aging step. 煮沸工程から熟成工程のいずれかにおいて桑葉を添加した、請求項4記載の麦芽アルコール飲料。   The malt alcoholic beverage according to claim 4, wherein mulberry leaves are added in any of the boiling step to the aging step.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102326812A (en) * 2010-07-13 2012-01-25 陈华玲 Mulberry leaf beverage and preparation method thereof
CN103998051A (en) * 2011-12-01 2014-08-20 雀巢产品技术援助有限公司 Morus berries and avoiding glucose peaks
CN109762867A (en) * 2019-03-25 2019-05-17 河南科技大学 Increase the fermentation processing method of 1-DNJ extracted amount in albumen mulberry
WO2020202749A1 (en) * 2019-04-04 2020-10-08 サントリーホールディングス株式会社 Containerized beverage containing 1-deoxynojirimycin

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102326812A (en) * 2010-07-13 2012-01-25 陈华玲 Mulberry leaf beverage and preparation method thereof
CN103998051A (en) * 2011-12-01 2014-08-20 雀巢产品技术援助有限公司 Morus berries and avoiding glucose peaks
CN109762867A (en) * 2019-03-25 2019-05-17 河南科技大学 Increase the fermentation processing method of 1-DNJ extracted amount in albumen mulberry
CN109762867B (en) * 2019-03-25 2022-05-31 河南科技大学 Fermentation treatment method for increasing extraction amount of 1-deoxynojirimycin in protein mulberry
WO2020202749A1 (en) * 2019-04-04 2020-10-08 サントリーホールディングス株式会社 Containerized beverage containing 1-deoxynojirimycin
JP2020167969A (en) * 2019-04-04 2020-10-15 サントリーホールディングス株式会社 Packed beverage containing 1-deoxynojirimycin

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