JP2009098039A - Analysis vessel and analysis device - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an analysis vessel capable of correctly measuring an optical path length even when reagents are supported in a reaction chamber. <P>SOLUTION: A reagent support area (22) to support a reagent (8) and an analysis area (21), adjacent to the reagent support area (22), where a mixture liquid flows into are provided in the single reaction chamber (5), therefore, analysis can be performed without effect of production variation by preliminarily measuring an optical path length of the analysis area (21) before flowing a mixture liquid in each analytical vessel having a production variation and compensating an absorbance of the analysis area (21), filled with the mixture liquid, by the optical path length. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to an analysis container used in an analyzer for analyzing the concentration of a specific component contained in a liquid sample in the medical field, the environmental field, or the like.

  2. Description of the Related Art Conventionally, an analyzer for analyzing a liquid sample such as blood is configured as seen in Patent Document 1, for example. As shown in FIG. 16, the sample liquid S injected into the liquid receiver 40 of the analysis container is transferred to the reaction chamber 41B of the analysis container by centrifugal force and capillary action through the flow path 41 of the analysis container. In the reaction chamber 41B, the reagent part 44 set in the reaction chamber 41B and the sample solution S are reacted to optically access the mixed solution in the reaction chamber 41B to read the color reaction of the mixed solution. It is configured.

The optical access of the analyzer means that the reaction chamber 41B in which a color reaction occurs due to the reagent being dissolved by the sample solution S is irradiated with a light source mounted on the analyzer, and the reflected light or transmitted light thereof. Is detected by the light receiving unit, the absorbance: ABS, which is the logarithm of the ratio of the irradiation light amount and the detected light amount,
ABS = log ₁₀ (I / O)
Here, I is an irradiation light amount (incident light amount), and O is a detected light amount (outgoing light amount).
Then, the concentration of the specific component in the liquid sample is converted from the relationship data between the absorbance and the concentration stored in advance in the apparatus, that is, a so-called calibration curve.

This analysis container is composed of a base in which various recesses forming the flow channel 41 and the reaction chamber 41B and the like are formed on the upper surface, and a cover bonded to the upper surface of the base with an adhesive layer. The reagent 44 is supported on 41B by dropping a required amount of a liquid reagent into the reaction chamber 41B before adhering the cover to the upper surface of the base, and after naturally drying or freeze-drying, the base and the cover are attached. The analysis container is completed by bonding with the adhesive layer.
JP 2004-150804 A (FIG. 10)

The concentration of the specific component contained in the sample solution S is converted by the method as described above, which is obtained according to the Lambert-Beer law.
ABS = ε ・ c ・ L
Here, ABS is the absorbance, ε is the molar extinction coefficient, c is the concentration of the measurement object, and L is the optical path length of the measurement object. As can be seen from this equation, even if the measurement object having the same concentration is measured, the absorbance includes an error in proportion to the variation due to the variation in the optical path length of the reaction chamber 41B of the analysis container. Therefore, the density converted from 1 also includes an error.

However, the optical path length variation occurs due to component variations or bonding variations that occur during the bonding process, and cannot be completely eliminated by manufacturing measures alone.
Therefore, in order to improve the analysis accuracy, it is necessary to actually measure the optical path length at the time of manufacture, write the actually measured value to the analysis container as analysis container information as a barcode, and perform correction at the time of analysis.

  However, in the conventional configuration, even if an optical path length is measured using a non-contact type position measuring device using a laser after bonding, the laser beam is inhibited by the reagent 44 carried in the reaction chamber 41B. The optical path length of the reaction chamber cannot be measured.

Further, when the optical path length is measured before the reagent is loaded, there is a problem that the optical path length cannot be measured correctly because the bonding variation is not taken into consideration.
An object of the present invention is to solve the above-mentioned conventional problems, and to provide an analytical container capable of correctly measuring an optical path length even when a reagent is carried in a reaction chamber in a production stage.

  The analysis container according to the present invention is an analysis container used for optical access to a liquid mixture of a liquid sample transferred to a reaction chamber and a reagent set in the reaction chamber, and the single reaction The chamber is provided with a reagent carrying area for carrying the reagent and an analysis area adjacent to the reagent carrying area and into which the mixed solution flows.

Further, it is characterized in that an unevenness is formed on at least one of the bottom surface and the upper surface of the single reaction chamber, and one of the unevenness is used as a reagent carrying region, and the other of the unevenness is used as an analysis region.
Further, the reagent carrying region and the analysis region at the same level are formed on at least one of the bottom surface and the top surface of the single reaction chamber, and a convex portion or a concave portion is formed at the boundary between the reagent carrying region and the analysis region. It is formed.

Further, the analysis region is subjected to a hydrophobic treatment.
Further, the present invention is characterized in that a plurality of reagent carrying regions for carrying the different types of reagents are provided in a single reaction chamber.

  According to this configuration, the reagent carrying region is provided in the single reaction chamber, and the analysis region through which the mixed liquid flows into the single reaction chamber is provided separately from the reagent carrying region. The optical path length can be measured by measuring the analysis region before sending the liquid sample into the reaction chamber. Then, the liquid sample sent into the reaction chamber and reacted with the reagent in the reagent carrying region can be received and analyzed in the analysis region.

Hereinafter, the analysis container of the present invention will be described based on each embodiment.
Here, the case of an analysis container used in an analyzer that analyzes the concentration of a specific component contained in a liquid sample such as blood will be described as an example.

(Embodiment 1)
1 to 5 show Embodiment 1 of the present invention.
As shown in FIGS. 1A and 1B, the analysis container according to the first embodiment is configured by attaching a cover 10 to a base 9. The bonding surface of the base 9 with the cover 10 is provided with a liquid sample storage chamber 4 for temporarily storing the liquid sample, and a plurality of optical detections for optically detecting the color reaction of the liquid sample and the reagent. Reaction chambers 5a, 5b, 5c and a waste liquid reservoir 6 for storing excess liquid samples are formed. Each of these chambers is formed by closing a recess opening formed in the base 9 with a cover 10. 3a is a first flow path for sending a liquid sample received from the liquid sample inlet 2 of the cover 10 to the liquid sample storage chamber 4, and 3b is a liquid sample from the liquid sample storage chamber 4 to the reaction chambers 5a, 5b, 5c and the waste liquid reservoir 6. Is formed by closing the opening of the recess formed in the base 9 with the cover 10.

  The base 9 is bonded to the cover 10 after the reagents 8a, 8b and 8c are carried in the recesses of the base 9 which become the reaction chambers 5a, 5b and 5c, followed by bonding of UV adhesive, hot melt, double-sided tape, etc. It is executed using the material. Further, a part of the base 9 and the cover 10 can be melted and joined using a laser or ultrasonic waves.

FIG. 2 shows details of the base 9, and FIG. 3 shows a cross section near the reaction chamber 5a indicated by BB 'in FIG. 2 after the base 9 and the cover 10 are bonded together by the adhesive layer 11. As shown in FIG.
An analysis region 21 having a higher level than the periphery is formed at the bottom of the concave portion of the reaction chamber 5 a, and a reagent 8 a is carried in a groove surrounding the analysis region 21, and this portion is adjacent to the analysis region 21. The reagent carrying region 22 is obtained.

  The recesses of the reaction chambers 5b and 5c are formed in the same manner, and the type of the reagent 8b carried in the groove surrounding the analysis region 21 of the reaction chamber 5b is different from that of the reagent 8a. The reagent 8c carried in the groove surrounding the analysis region 21 of the reaction chamber 5c is different from the reagents 8a and 8b.

  FIG. 4 (a) shows a step of loading the reagent 8 in the groove surrounding the analysis region 21, and a liquid reagent 8a is dropped in the reagent loading region 22 by a necessary amount by the reagent applicator 12, and then naturally dried. Alternatively, it is solidified and fixed by freeze-drying.

  FIG. 4 (b) shows the CC ′ cross section of FIG. 4 (a), and the depth d of the grooves 7a, 7b, 7c that form the reagent carrying regions 22 of the reaction chambers 5a, 5b, 5c is 50 μm or more. If present, it is suitable for carrying a reagent. Similarly, the reagents 8b and 8c are carried in the groove 7b of the reaction chamber 5b and the groove 7c of the reaction chamber 5c.

  It should be noted that each analysis region 21 of the reaction chambers 5a, 5b, and 5c is subjected to a hydrophobic treatment in advance so that the reagent does not adhere to the analysis region 21 when the reagents 8a, 8b, and 8c are supported on the reagent support region 22. preferable.

  Thus, the optical path length of each analysis region 21 of the reaction chambers 5a, 5b, and 5c in the analysis container 1 carrying the reagent is measured using the laser beam, and the actual value of the obtained optical path length is the analysis container information. As a barcode and printed on the analysis container.

  When the optical path length is measured from the side of the cover 10 as shown in FIG. 5A, the cover 10 is made of a material that transmits the wavelength of the laser light emitted from the laser length measuring instrument 23, and later. It is formed of a material that also transmits light having a wavelength of a light source such as a light-emitting diode that is used during analysis by the analyzer described. The base 9 does not need to transmit light having the above-mentioned wavelength, but in order to detect a color reaction at the time of analysis by the analyzer, it is necessary to secure a constant amount of light incident on the light receiving unit. Here, when the measured distance L1 of the analysis region 21 detected by the laser length measuring device 23 and the measured distance L2 of the inner surface 10a of the cover 10 detected by the laser length measuring device 23, the optical path length (L1-L2). Is printed as a barcode on the analysis container.

  When using a material that does not transmit light for the material of the base 9 and measuring the color reaction in a reflective manner at the time of analysis with the analyzer, the surface of each analysis region 21 is vapor-deposited such as aluminum, Light passing through the reaction chamber needs to be reflected to the cover 10 side and detected by a light receiving unit disposed on the cover 10 side. Both the base 9 and the cover 10 may be made of a material that transmits light.

  When the optical path length is measured from the side of the base 9 as shown in FIG. 5B, the base 9 is made of a material that transmits the wavelength of the laser beam used for the measurement, and is analyzed by the analyzer. It is also necessary to transmit light having the wavelength of the light source used. The cover 10 does not need to transmit light, but when a material that does not transmit light is used as the material of the cover 10, the light passing through the reaction chamber is made to pass through the reaction chamber by evaporating aluminum or the like on the cover 10. It is necessary to detect with a light receiving part that is reflected to the side and arranged on the base 9 side. Both the base 9 and the cover 10 may be made of a material that transmits light.

  Here, when the measured distance L2 of the analysis region 21 detected by the laser length measuring device 23 and the measured distance L1 of the inner surface 10a of the cover 10 detected by the laser length measuring device 23, the optical path length (L1-L2). Is printed as a barcode on the analysis container.

The method for recording the analysis container information is not limited to the barcode, and a data carrier such as an IC tag that records the optical path length information may be attached to the analysis container.
Using the analysis container 1 carrying the reagent in this way, analysis processing is performed as follows.

  A blood plasma component or the like is used for the liquid sample, and a certain amount of blood plasma component separated by a centrifuge is extracted with a microbipet or the like and injected from the liquid sample inlet 2. The liquid sample injected from the liquid sample inlet 2 is transferred to the liquid sample storage chamber 4 by capillary action. Subsequent liquid sample transfer operations and analysis are performed in the analyzer after the analysis container is inserted into the analyzer.

  As shown in FIG. 6, the analysis container 1 is set on the turntable 103 of the analyzer 100 at a position away from the rotating shaft 102 of the turntable 103. Reference numeral 104 denotes a motor that drives the turntable 103 around the rotation shaft 102, and is attached at an angle θ from the vertical direction. The turntable 103 is provided with openings 51 and 52, and the light emitted from the light emitting diode 105 indicates the position of each analysis region 21 in the reaction chambers 5a, 5b, and 5c of the analysis container 1 set on the turntable 103. It arrange | positions so that it may permeate | transmit and detect with the photodetector 106. FIG.

  As the turntable 103 rotates, a centrifugal force is generated in the direction of arrow A as shown in FIG. 2, the liquid sample inside the liquid sample storage chamber 4 is transferred, and the liquid sample is transferred to the reaction chambers 5a, 5b, 5c. It is carried.

  When the liquid sample flows into the reaction chambers 5a, 5b, and 5c, the reagents 8a, 8b, and 8c are dissolved by the liquid sample, and a color reaction occurs according to the components. At this time, as shown in FIG. 7, the analysis region 21 must be filled with the mixed solution 15 of the reagent and the liquid sample in the optical path length direction so that there is no gap due to bubbles. If there are bubbles in the reaction chamber, the analysis region 21 is filled with the mixed solution 15 so as not to generate a gap in the optical path length direction by using the centrifugal force of rotation to bring the mixed solution 15 in one direction. There is a need. The analyzer 100 performs reading at the timing when the reaction region 5a, 5b, 5c passes between the light emitting diode 105 and the photodetector 106 in a state where the analysis region 21 of the reaction chamber 5a, 5b, 5c is filled with the mixed solution 15, and the absorbance and analysis at this time are performed. The concentration of the specific component in the liquid sample is calculated from the information on the optical path length of each reaction chamber 5a, 5b, 5c read from the container 1.

  In this way, the optical path length can be measured without being hindered by the reagent even though the reagent is carried in the reaction chamber. Therefore, even if the thickness of the adhesive layer 11 varies due to variations in the bonding process, the analysis results Can be derived more accurately.

(Embodiment 2)
In the first embodiment shown in FIG. 3, the height of the analysis region 21 is formed higher than that of the reagent carrying region 22, but this point is different in the second embodiment of the present invention shown in FIG. .

  FIG. 8A shows a plan view of the base 9 of the analysis container 1 configured by bonding the base 9 and the cover 10, and FIG. 8B shows a DD of the reaction chamber 5 in FIG. 8A. FIG. 8C is a cross-sectional view after applying the cover, along the cross-section before applying the reagent.

  In the second embodiment, as shown in FIGS. 8A and 8B, the analysis region 21 and the reagent carrying region 22 are formed at the same level, and the convex portion 24 is formed at the boundary between the analysis region 21 and the reagent carrying region 22. Also, the reagent 8a is dropped into the reagent holding region 22 by the required amount by the reagent coating machine 12 and supported as shown in FIG. 8C, so that the reagent 8a enters the analysis region 21. I can stop. The same applies to the reaction chambers 5b and 5c.

(Embodiment 3)
In the first embodiment shown in FIG. 3, the height of the analysis region 21 is formed higher than that of the reagent carrying region 22, but this point is different in the third embodiment of the present invention shown in FIG. .

  FIG. 9A shows a plan view of the base 9 of the analysis container 1 configured by bonding the base 9 and the cover 10, and FIG. 9B shows the EE of the reaction chamber 5 in FIG. 9A. 'Is a cross-sectional view before applying a reagent, and Fig. 9 (c) is a cross-sectional view after attaching a cover.

  In the third embodiment, as shown in FIGS. 9A and 9B, the analysis region 21 and the reagent carrying region 22 are formed at the same level, and a recess 25 is formed at the boundary between the analysis region 21 and the reagent carrying region 22. The reagent can be prevented from entering the analysis region 21 by dropping a required amount of the liquid reagent onto the reagent carrying region 22 by the reagent coating machine 12 and carrying it as shown in FIG. 9C. . The same applies to the reaction chambers 5b and 5c.

(Embodiment 4)
In each of the above embodiments, the reagent carrying region 22 is surrounded by the outside of the analysis region 21, but this point is different in the fourth embodiment of the present invention shown in FIG.

  FIG. 10A shows a plan view of the base 9 of the analysis container 1 configured by bonding the base 9 and the cover 10, and FIG. 10B shows the FF in the reaction chamber 5c of FIG. 10A. ′ Shows a cross section.

  In the fourth embodiment, as shown in FIG. 10A, analysis is performed at the outermost end in the direction A of the centrifugal force generated when the analysis container 1 is set on the turntable 103 and rotated in the reaction chamber 5c. A reagent carrying region 22 deeper than the region 21 is formed, and the reagent 8c is carried on the reagent carrying region 22. The same applies to the reaction chambers 5a and 5b.

  In the fourth embodiment, the case where the heights of the analysis region 21 and the reagent carrying region 22 are different from each other has been described as an example. However, the analysis region 21 and the reagent carrying region 22 are formed to have the same height. 8 can be realized by separating the analysis region 21 and the reagent-carrying region 22 by providing the convex portion 24 seen in FIG. 8 of the second embodiment in a direction crossing the direction A of the centrifugal force.

  In the fourth embodiment, the case where the heights of the analysis region 21 and the reagent carrying region 22 are different from each other has been described as an example. However, the analysis region 21 and the reagent carrying region 22 are formed to have the same height. 9 can be realized by separating the analysis region 21 and the reagent carrying region 22 by providing the recess 25 seen in FIG. 9 of the third embodiment in a direction crossing the direction A of the centrifugal force.

(Embodiment 5)
In each of the above-described embodiments, the reagent carrying region 22 surrounds the outside of the analysis region 21, but this point is different in the fifth embodiment of the present invention shown in FIG.

  FIG. 11A shows a plan view of the base 9 of the analysis container 1 configured by bonding the base 9 and the cover 10, and FIG. 11B shows GG in the reaction chamber 5 c of FIG. 11A. ′ Shows a cross section.

  In the fifth embodiment, as shown in FIG. 11A, in the reaction chamber 5c, the direction of centrifugal force generated when the analysis container 1 is set on the turntable 103 and rotated in the center of the reaction chamber 5c. An analysis region 21 is formed along A, and a reagent carrying region 22 deeper than the analysis region 21 is formed on both sides of the analysis region 21 along the centrifugal force direction A, as shown in FIG. The reagent 8c is carried on the reagent carrying region 22. The reagent carrying region 22 may be formed not on both sides of the analysis region 21 but on one side.

  In the fifth embodiment, when the reagent carrying regions 22 are formed on both sides of the analysis region 21, the types of reagents carried on the two reagent carrying regions 22 are the same, but the reagent carrying formed on both sides of the analysis region 21 is the same. The type of reagent carried on the region 22 may be different.

  In the fifth embodiment, the case where the heights of the analysis region 21 and the reagent carrying region 22 are different from each other has been described as an example. However, the analysis region 21 and the reagent carrying region 22 are formed to have the same height. 8 can be realized by separating the analysis region 21 and the reagent carrying region 22 by providing the convex portions 24 seen in FIG. 8 of the second embodiment in the direction along the direction A of the centrifugal force.

  In the fifth embodiment, the case where the heights of the analysis region 21 and the reagent carrying region 22 are different from each other has been described as an example. However, the analysis region 21 and the reagent carrying region 22 are formed to have the same height. 9 can be realized by separating the analysis region 21 and the reagent-carrying region 22 by providing the recesses 25 seen in FIG. 9 of the third embodiment in the direction along the direction A of the centrifugal force.

(Embodiment 6)
In each of the above-described embodiments, the reagent carrying region 22 is formed only on the base 9 side of the analysis container 1 configured by bonding the base 9 and the cover 10, but Embodiment 6 of the present invention shown in FIG. So this is different.

  FIG. 12A shows a cross-sectional view of the analysis container 1 constructed by bonding the base 9 and the cover 10 at the same position as that in FIG. 3, and the analysis of the base 9 is performed not only on the base 9 side but also on the cover 10 side. It is also possible to form the groove 26 at a position not facing the region 21 and to carry the reagent 8d in the groove 26. The same applies to the reaction chambers 5a and 5b.

In the sixth embodiment, the reagent 8a on the base 9 side and the reagent 8d on the cover 10 side may be the same or different.
In the above description of the sixth embodiment, the modification of the first embodiment has been described. However, in each of the second to fifth embodiments, the analysis region 21 is also provided on the cover 10 side. It is also possible to form a groove at a position that does not face the substrate and to carry a reagent in this groove.

  In the above description of the sixth embodiment, the modification of the first embodiment has been described. However, in each of the second to fifth embodiments, an analysis region is also provided on the cover 10 side. A convex portion or a concave portion that divides the reagent carrying region can be formed, and the reagent can also be carried on the cover 10 side.

(Embodiment 7)
In the analysis container of the first embodiment, the height of the analysis region 21 is formed higher than that of the reagent carrying region 22, but this point is different in the seventh embodiment of the present invention shown in FIG. .

  The plan view of the base 9 of the seventh embodiment is the same as FIG. 2A, and the cross section BB ′ of the reaction chamber 5 is as shown in FIG. Is also formed low. The rest is the same as in the first embodiment. The same applies to the reaction chambers 5a and 5b.

(Embodiment 8)
In the analysis container of each of the above embodiments, the reagent carried in a single reaction chamber dissolves and reacts with the liquid sample filled in the reaction chamber at a time, and then the light transmission amount of the analysis region is analyzed by the analyzer. However, in the eighth embodiment of the present invention shown in FIG. 14 and FIG. 15, there are two steps as a reaction step between a reagent and a liquid sample. Shows when necessary.

The process will be described later.
FIG. 14A is a perspective view of the main part of the base 9 of the analysis container 1 configured by bonding the base 9 and the cover 10 together. FIG. 14B shows a cross-sectional view of the analysis container 1 set on the turntable 103 of the analyzer 100. The base 9 in FIG. 14B shows the HH ′ cross section in FIG.

  As shown in FIG. 14 (a), in the recess of the base 9 that becomes the reaction chamber 5 a, a liquid receiving portion 28 formed as an analysis region 21 at the outermost peripheral portion with respect to the rotation center 27 of the turntable 103, and the turntable A first reagent carrying region 22a and a second reagent carrying region 22b as reagent carrying regions 22 are formed adjacent to the liquid receiving unit 28 and on the inner peripheral side of the liquid receiving unit 28 with respect to the rotation center 27 of 103. Yes.

  The liquid receiving portion 28 and the first and second reagent carrying regions 22a and 22b are closed by the cover 10 as shown in FIG. 9B to form a reaction chamber 5a, and the first and second reagent carrying regions 22a. , 22b and the inner surface of the cover 10 is formed with a gap 29 in which a capillary force acts on the liquid sample. The first reagent carrying region 22 a carries the first reagent 8 aa before the cover 10 is attached to the base 9. A second reagent 8ab of a different type from the first reagent 8aa is carried on the second reagent carrying region 22b before the cover 10 is attached to the base 9.

  In a state where the liquid sample is not received in the liquid receiving portion 28, the optical path length L is measured using the laser length measuring machine 23 in the same manner as in FIG. 5, and the optical path length L is printed as a barcode on the analysis container 1. Or record it on a data carrier attached to the analysis container.

Using the analysis container 1 carrying the first and second reagents 8aa and 8ab in this way, analysis processing is performed as follows.
A blood plasma component or the like is used as the liquid sample, and a certain amount of the blood plasma component separated by the centrifuge is extracted with a microbipet or the like and injected into the analysis container 1. The injected liquid sample is transferred to the liquid receiving part 28 through the flow path 3 as shown in FIG. 15A due to the capillary action and the centrifugal force generated by the rotation of the turntable 103. Next, as shown in FIG. 15B, the turntable 103 is stopped at the position where the first reagent carrying region 22a is positioned downward. As a result, the liquid sample 30 of the liquid receiving portion 28 is held in the gap 29 of the first reagent holding region 22a, and the turntable 103 is stopped for a specified time in this state, so that the liquid sample 30 has the first reagent. 8aa melts and reacts (first reaction).

  Next, when the turntable 103 is rotated, the liquid mixture 31 held in the gap 29 of the first reagent carrying region 22a is transferred to the liquid receiving portion 28 by the centrifugal force as shown in FIG.

  Next, as shown in FIG. 15D, the turntable 103 is stopped at the position where the second reagent carrying region 22b is positioned downward as shown in FIG. As a result, the mixed solution 31 of the liquid receiving portion 28 is held in the gap 29 of the second reagent holding region 22b, and the turntable 103 is stopped for a specified time in this state, so that the second reagent is added to the mixed solution 31. 8ab further dissolves and reacts (second reaction) to become a mixed solution 32.

  Next, when the turntable 103 is rotated, the liquid mixture 32 held in the gap 29 of the second reagent carrying region 22b is transferred to the liquid receiving portion 28 by the centrifugal force as shown in FIG. Reading is performed at a timing when the reaction chamber 5a filled with the mixed solution 32 in which the second reagent 8ab is dissolved passes through the space between the light emitting diode 105 and the photodetector 106 of the analyzing apparatus 100. The concentration of the specific component in the liquid sample is calculated from the absorbance of the light and the information of the optical path length L of the reaction chamber 5a read and held from the analysis container 1 in advance.

  In this way, the optical path length can be measured without being disturbed by the reagent despite the first and second reagents 8aa and 8ab being carried in the reaction chamber 5a. Even if thickness variations occur, analysis results can be derived more accurately. In addition, since a plurality of reagent-carrying regions 8aa and 8ab carrying different types of reagents are provided in a single reaction chamber 5a, the analysis of specific components required for two steps as a reaction step between the reagent and the liquid sample can be performed. By controlling the posture of the analysis container 1 by the apparatus 100, it can be carried out using only a single reaction chamber 5a. The same applies to the reaction chambers 5b and 5c.

  In each of the above-described embodiments, the blood cell separation process, which is a pretreatment for analysis, is performed outside the analysis container 1. However, after the analysis container 1 is set in the analyzer 100, separation is performed by rotation control of the turntable 103. It can also be configured so as to be transferred to the reaction chambers 5a, 5b, 5c.

  In each of the above embodiments, the liquid sample is transferred to the reaction chambers 5a, 5b, and 5c using the centrifugal force accompanying the rotation of the turntable 103. Can also be configured to be transferred to the reaction chamber.

  In the first to fifth embodiments and the seventh embodiment, irregularities are formed on the bottom surfaces of the single reaction chambers 5a, 5b, and 5c (base 9 side) and the top surface (cover 10 side). One of the concaves and convexes is used as a reagent-carrying region and the other of the concaves and convexes is used as an analysis region. The bottom surface (base 9 side) and the top surface (cover 10 side) of the single reaction chambers 5a, 5b, and 5c. Concavities and convexities are formed on the upper surface, and one of the concavities and convexities can be used as a reagent carrying region, and the other concavity and convexity can be configured as an analysis region.

  INDUSTRIAL APPLICABILITY The present invention can contribute to improving the analysis accuracy of an analyzer that measures the concentration of various components in a sample collected from a living organism or sewage.

The exploded perspective view and assembly perspective view of the analysis container in Embodiment 1 of this invention Plan view of base in the same embodiment The expanded sectional view of the principal part along the BB 'line of FIG. The perspective view of the reagent application | coating process in the same embodiment, and its CC 'sectional drawing Sectional drawing explaining the relationship between the liquid mixture of a reagent and a liquid sample, and an optical path length in the embodiment Sectional drawing of the analyzer which set the analysis container in the embodiment Sectional view during analysis in the analyzer with the analysis container set in the same embodiment Plan view before attaching the cover of the reaction chamber of the analysis container according to the second embodiment of the present invention, and sectional view before and after applying the reagent along the DD ' Plan view before attaching the cover of the reaction chamber of the analysis container according to Embodiment 3 of the present invention, and sectional view before applying the reagent along the line EE ′ and after attaching the cover Plan view of base and sectional view along FF ′ in embodiment 4 of the present invention Plan view of base and sectional view along GG 'in embodiment 5 of the present invention Sectional drawing of the analytical container of Embodiment 6 of this invention Sectional drawing of the reaction chamber of the analysis container of Embodiment 7 of this invention The perspective view of the base side of the reaction chamber of the analysis container of Embodiment 8 of this invention, and sectional drawing of the reaction chamber of the analysis container set to the analyzer Explanatory drawing of the attitude | position control process of the analysis container by the analyzer of the embodiment Plan view of a conventional analytical container

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Analysis container 2 Liquid sample inlet 3a 1st flow path 3b 2nd flow path 4 Liquid sample storage chamber 5a, 5b, 5c Reaction chamber 6 Waste liquid reservoir 7a, 7b, 7c Groove 8a, 8b, 8c, 8d Reagent 8aa, 8ab First and second reagents 9 Base 10 Cover 11 Adhesive layer 12 Reagent applicator 21 Analysis area 22 Reagent carrying area 22a First reagent carrying area (reagent carrying area)
22b Second reagent carrying area (reagent carrying area)
23 Laser length measuring machine 24 Convex part 25 Concave part 26 Groove 27 Rotation center 28 of turntable 103 Liquid receiving part (analysis region)
29 Clearance 30 Liquid sample 31, 32 Mixed solution 51, 52 Opening 100 Analyzer 102 Rotating shaft 103 Turntable 104 Motor 105 Light emitting diode 106 Photo detector A Direction of centrifugal force L Optical path length

Claims (6)

An analytical container used for reading to optically access a liquid mixture of a liquid sample transferred to a reaction chamber and a reagent set in the reaction chamber,
In a single said reaction chamber,
A reagent carrying region carrying the reagent;
An analysis container provided with an analysis region adjacent to the reagent carrying region and into which the mixed solution flows. The analysis container according to claim 1, wherein irregularities are formed on at least one of a bottom surface and an upper surface of the single reaction chamber, one of the irregularities is used as a reagent carrying region, and the other of the irregularities is used as an analysis region. The reagent carrying region and the analysis region having the same level are formed on at least one of the bottom surface and the top surface of the single reaction chamber, and a convex portion or a concave portion is formed at the boundary between the reagent carrying region and the analysis region. The analysis container according to claim 1. The analysis container according to claim 2 or claim 3, wherein the analysis region is subjected to a hydrophobic treatment. The analysis container according to claim 1, wherein a plurality of reagent carrying regions for carrying the different types of reagents are provided in a single reaction chamber. The analysis container according to any one of claims 1 to 5,
A rotating body having a rotation axis and holding the analysis container;
A rotation drive unit that rotates the rotating body so that centrifugal force acts on the analysis container;
And an analyzer that optically accesses and measures the liquid in the operation chamber of the analysis container.

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