JP2006258696A - Analysis disc and analyzer using the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To overcome the problem that it is difficult for a conventional analysis disc to implement an aerobic reaction. <P>SOLUTION: A first chamber 3 accommodates a liquid sample on the axial center side. A second chamber 12 is provided outside the first chamber 3. A third chamber 8 is provided between the first and second chambers. The first and third chambers 3, 8 are coupled by a flow path 6. The second and the third chambers 12, 8 are coupled by a flow path 11. The third chamber 8 has a cross section larger than cross sections of the first and second flow paths in the direction perpendicular to a flow of the liquid sample, and also has a volume larger than a volume of the liquid sample flowing into the third chamber 8. A barrier 10 is disposed within the third chamber 8, and irregularly disturbs the flow of the sample liquid. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to an analysis disk and an analysis apparatus, and more particularly to a technique for promoting an aerobic reaction during analysis.

 A general method for measuring the concentration of components in blood using an analysis disk will be described below.

 The basic structure of the analysis disk used for the analysis of a liquid sample such as blood in the prior art is the basic structure of the chamber and the flow path, the first chamber and the second chamber provided in the outer peripheral direction from the axial center of the chamber. Are connected by a flow path having a capillary force (force by which a liquid travels in the flow path due to a capillary phenomenon).

 First, a specimen is injected into the first chamber. Thereafter, by rotating the disk, centrifugal force is applied from the axial center to the outer periphery of the disk to centrifuge red blood cells in the blood. Thereafter, when the rotation is stopped and the centrifugal force is eliminated, a part of the liquid sample in the first chamber, for example, plasma moves through the flow path and advances through the flow path to just before the second chamber by capillary force. At this time, the second chamber has a larger cross-sectional area than the flow path, and the specimen does not flow into the second chamber due to the surface tension of the specimen. Next, the specimen is moved into the second chamber by rotating the disk again and applying a centrifugal force.

In this second chamber, a reagent that causes a reaction with an arbitrary component in the specimen and causes a color change in accordance with the concentration of the component is disposed. By optically measuring the color change, Any arbitrary component concentration can be measured.
As prior art document information related to the invention of this application, for example, Patent Document 1 is known.
Japanese National Patent Publication No. 10-501340

 However, such an analytical disk has a problem that it is difficult to use an aerobic reaction, that is, a reaction that consumes oxygen, as a reaction for detecting an arbitrary component in a specimen.

 The chamber inside the conventional analysis disk is substantially closed to the outside air, and the air vent for escaping the air in the chamber is very small, which is insufficient to supply the oxygen necessary for the reaction in the sample. It is. When an aerobic reaction is caused in such an environment, the reaction system causes oxygen deficiency particularly in the measurement of a high concentration region of a component to be measured in which the reaction proceeds actively, and the response of the reaction is lowered. Therefore, anaerobic reaction is used in the conventional disk type reaction vessel.

 However, enzymes used for anaerobic reactions (eg, dehydrogenases) are generally more expensive than enzymes used for aerobic reactions (eg, oxidases), and easily used in aerobic reactions. Even if the reaction can be realized, an anaerobic reaction may require a more complicated reaction system, increasing the number of reagents and increasing the difficulty of controlling the reaction. Furthermore, for analysis objects that do not have a means for measuring anaerobic reactions, the analysis can be given up or an aerobic reaction can be selected to avoid a decrease in sensitivity in the high-concentration region by correction. Met.

 Therefore, the present invention solves this problem, and uses a disk-type reaction vessel provided with a chamber shaped to efficiently dissolve oxygen in a specimen, so that an aerobic reaction can be used in a wide concentration region of a substance to be measured. The purpose is to improve the responsiveness of the reaction.

 In order to achieve this object, an analysis disk according to the present invention includes a first chamber in which a liquid sample provided on an axial center side is accommodated, and an outer side than the first chamber with respect to the axial center. A second chamber provided; a third chamber provided between the first and second chambers; the first chamber and the third chamber connected to each other; Connecting the first flow path for transferring the liquid sample to the third chamber, the third chamber and the second chamber, and the liquid sample contained in the third chamber being A second flow path for transferring to the second chamber, wherein the third chamber has a cross-sectional area of the first and second flow paths in a direction perpendicular to the flow of the liquid sample. With a larger cross-sectional area than The liquid chamber has a volume larger than the volume of the liquid sample transferred from the first chamber and flowing into the third chamber, and the flow of the liquid sample is irregularly disturbed inside the third chamber. By arranging obstacles for the purpose, the initial purpose can be achieved.

As described above, the analysis disk according to the present invention is in the middle of the flow path connecting the first chamber and the second chamber, and in order to efficiently dissolve the components in the gas in the specimen by centrifugal force, It has a third chamber that is thicker in the height direction than the flow path and larger in volume than the liquid sample into which it has flowed, and an obstacle that makes the flow of the liquid sample irregular in the third chamber. Since the sample is provided, the liquid sample passes through the third chamber while the sample is being transferred from the first chamber to the second chamber by centrifugal force. Reaction responsiveness can be improved in a wide concentration range of the substance to be measured in the reaction.
If such a chamber having the function of taking oxygen into the sample is used for analysis of the sample component concentration, the production cost for the analysis disk can be reduced compared with the case of using an anaerobic reaction, In addition, the reaction system can be simplified, and the degree of freedom in selecting the analysis target can be increased.

(Embodiment 1)
Hereinafter, Embodiment 1 of the present invention will be described with reference to the drawings. FIG. 1A is a plan view of a chamber and a flow path inside an analysis disk provided with a chamber for dissolving oxygen in the liquid sample in the first embodiment, and FIG. It is sectional drawing of the chamber which dissolves oxygen. In FIG. 1, 3 is a first chamber (container), and 1 is a liquid inlet provided at the upper end of the first chamber 3. Reference numeral 2 denotes an air vent provided at the upper end of the first chamber 3.

 Reference numeral 12 denotes a second chamber. The second chamber 12 is located outside the axial center located above the first chamber 3 in the figure with respect to the direction of the centrifugal force indicated by the arrow 7. ing. Reference numeral 13 denotes an air vent provided at the upper end of the second chamber 12.

 Reference numeral 8 denotes a third chamber, which is located in the middle of the flow path connecting the first chamber 3 and the second chamber 12. 6 is a flow path connecting the first chamber 3 and the third chamber 8, and is connected to the upper end of the third chamber 8 from an inlet 16 provided on the side surface of the first chamber 3. . The flow path 6 is bent in an inverted “U” shape, and the apex 6 a of the inverted “U” is positioned at least on the axial center side from the upper end of the liquid sample 4 entering the first chamber 3. . Further, the inverted “U-shaped” top portion 6 a is located at least upstream of the third chamber 8. 9 is an air vent provided at the upper end of the third chamber 8, and 11 is a flow path connecting the third chamber and the second chamber.

 Here, as shown in FIG. 1, the flow path 6 has a thickness 14 of 100 μm. This is a dimension for generating a capillary force (force that causes the liquid to travel through the flow path by capillary action) in the flow path 16. The first chamber 3, the second chamber 12, and the third chamber 8 each have a thickness dimension of 200 to 600 μm. This dimension is a dimension for preventing capillary force from being generated (non-generated) in the first chamber 3, the second chamber 12, and the third chamber 8. The wall surfaces of the flow paths 6 and 11 are made of a hydrophilic material. On the other hand, the wall surfaces of the first chamber 3, the second chamber 12, and the third chamber 8 are formed of a hydrophobic (water repellent) material. This is preferable in improving the capillary force.

 Further, as shown in FIG. 1B, the third chamber 8 is provided with an obstacle 10 that partially obstructs the movement of the liquid sample inside the chamber, and has a thickness dimension of 100 to 600 μm. Yes. The thickness dimension 15 of the third chamber 8 is at least 100 μm larger than the thickness dimension 14 of the flow path 6, and the thickness dimension 17 of the obstacle 10 is equal to or greater than the thickness dimension 14 of the flow path 6. The thickness dimension of the chamber 8 is 15 or less. The volume of the third chamber 8 is such that the volume of the space excluding the obstacle 10 is at least larger than the volume of the liquid sample entering the chamber from the first chamber, and the chamber is filled with the liquid sample. Don't be stupid. In this way, when the liquid sample passes through the third chamber 8, the liquid sample and air always exist in the chamber at the same time, and the flow control of the liquid sample by the obstacle 10 The contact area between the liquid sample and the air in the chamber is increased.

 FIG. 9 shows another example of the third chamber 8. The size and number of obstacles 10 can be selected optimally depending on the viscosity of the liquid sample. For example, when the viscosity of the liquid sample is low, the arrangement density of the obstacles 10 is increased as shown in FIG. And a configuration that strongly inhibits the movement of the liquid sample. When the viscosity of the liquid sample is high, the arrangement density of the obstacles 10 is lowered as shown in FIG. The optimum configuration can be selected.

 In FIG. 9, the obstacle 10 is integrally formed on a substrate called an upper cover formed with a flow path and a chamber, which is bonded to a base substrate serving as a substrate, but is not integrated with a wall surface in the third chamber. Alternatively, it may be formed separately. The material of the obstacle 10 when the obstacle 10 is not integrated with the cover disk is selected from polymer materials typified by glass, polycarbonate, polystyrene, etc., and among them, a material that does not inhibit the chemical reaction. The

 The operation of the analysis disk provided with the third chamber 8 configured as described above will be described below. First, as shown in FIG. 1, the liquid chamber 4 is filled in the first chamber 3. Then, the liquid sample 4 in the first chamber 3 passes through the inflow port 16 by capillary force and tries to fill the channel 6. At this time, since the thickness dimension of the liquid sample stopper 5 is at least 100 μm larger than the thickness dimension 14 of the flow path 6, the surface tension of the liquid sample 4 that attempts to minimize the gas-liquid surface area is reduced. The movement stops at the entrance of the liquid sample stopper 5, and the entire flow path 6 is not filled with the liquid sample 4.

 Next, as shown in FIG. 2, when a centrifugal force generated by rotating the analytical disk is applied from the axial center to the outer periphery of the disk as indicated by an arrow 7, the liquid sample 4 in the first chamber 3 is applied to the liquid sample 4 in the first chamber 3. A force is applied in the direction of the centrifugal force. As a result, the surface tension applied to the entrance of the liquid sample stopper 5 is broken, and the liquid sample 4 passes through the liquid sample stopper 5. The liquid sample 4 moves through the flow path 6 until the liquid level 4b is the same as the distance from the axial center to the liquid level 4a of the liquid sample 4 in the first chamber 3.

 Next, when the rotation of the disk is stopped and the centrifugal force is eliminated, as shown in FIG. 3, the liquid sample 4 passes through the inverted “U-shaped” top portion 6a by the capillary force and fills the entire flow path 6. Although the liquid sample 4 reaches the inlet 18 of the third chamber 8, the thickness dimension 15 of the third chamber 8 is larger than the thickness dimension 14 of the flow path 6. The sample 4 does not flow into the third chamber 8.

 Next, when the centrifugal force generated by rotating the analytical disk is applied again from the shaft center to the outer periphery of the disk as shown by the arrow 7, the liquid sample 4 is obtained as shown in FIGS. 4 (a) and 4 (b). , Passes through the third chamber 8 and the flow path 11, and as a result, moves to the second chamber 12 as shown in FIG.

 The number of rotations of the disk here varies depending on the shape and number of third chambers 8, the connection method, and the position and number of obstacles 10, but is generally in the range of 500 to 4000 rpm. For example, when the number of obstacles 10 is large and the disks are arranged relatively densely, the optimum rotational speed of the disk is fast, and when the number of obstacles 10 is relatively few and arranged sparsely, the disk is optimum. Speed is slow.

 At this time, in the process in which the liquid sample 4 passes through the third chamber 8, as shown in FIG. 10A, the liquid sample 4 is moved in the centrifugal force direction by the obstacle 10 inside the third chamber 8. Is prevented, and starts to diffuse widely within the third chamber 8. Then, as shown in FIG. 10B, the liquid sample 4 in the third chamber 8 moves in the centrifugal force direction, whereas the air in the third chamber moves in the centrifugal force source direction. As a result, bubbles are generated in the liquid sample 4 in the third chamber 8, and oxygen present in the third chamber 8 is efficiently dissolved in the liquid sample 4.

 Thereafter, an aerobic reaction is allowed to proceed inside the second chamber 12 to cause a color reaction according to the concentration of the analyte in the liquid sample 4, and the resulting change is measured by transmitted light or reflected light. And determine the concentration of the analyte. Note that the measurement method here is not limited to optical measurement, but instead of color development reaction, electron transfer is caused according to the concentration of the analyte, and the electron transfer is directly measured electrochemically. The concentration of the analyte can also be determined.

 Therefore, the analytical disk having the third chamber 8 can efficiently tackle oxygen into the liquid sample. Therefore, even if an aerobic reaction requiring oxygen is caused inside the disk, oxygen deficiency is caused. It is less likely to occur and good reaction characteristics can be obtained in a wide concentration range of the analyte, and the reaction characteristics can be improved particularly in the measurement of a high concentration area of the analyte that consumes a large amount of oxygen.

 FIG. 5 is a side view of an apparatus for using the analysis disk described in the first embodiment. In FIG. 5, reference numeral 21 denotes a disk motor as drive means provided connected to the shaft center 23 of the disk 22, and the disk 22 is rotated by the disk motor 21. The disk motor 21 is controlled and stopped by a control circuit 24 as control means.

 Reference numeral 25 denotes a laser light output drive circuit. A laser diode that forms the light emitting portion 26 emits light by the output of the laser light output drive circuit 25, and the laser light output at this time is directed toward the measurement chamber 36 of the disk 22. Fired. The measurement chamber 36 corresponds to the first to third chambers described above. Moreover, the laser beam output here can output laser beams having a plurality of wavelengths.

 Of this laser light, the laser light used for controlling the rotation of the disk 22 is reflected by the disk 22 and the information on the disk 22 is detected by the photodetector 27. The output is input to the control circuit 24, and the rotation of the disk 22 is controlled. That is, the rotation speed is controlled and stopped.

 Further, laser light passing through the measurement chamber 36 of the disk 22 among the laser light is detected by a photodetector forming the light receiving unit 28, and its output is processed by the video signal processing circuit 29. In this way, the absorbance of the liquid sample (liquid for component inspection) 35 in the measurement chamber 36 is measured.

 The disk 22 has a disk shape, the diameter is 8 cm or 12 cm, and the thickness 30 is 2 mm. The rotational speed of the disk 22 that generates the centrifugal force is 500 to 8000 revolutions per minute. The disk 22 also stores thickness information and calibration information of the measurement chamber 36, and the video signal processing circuit 29 measures the absorbance of the liquid sample 35 based on this information.

FIG. 6 is a cross-sectional view of the main part in the vicinity of the measurement chamber 36. In FIG. 6, reference numeral 31 denotes a base substrate. The base substrate 31 is made of polycarbonate (PC), glass, acrylic, plastic or the like, and is made of a transparent material.
An upper cover 33 is bonded to the upper surface of the base substrate 31 via an adhesive (two-component mixed adhesive, thermosetting adhesive, glue, etc.) 32. The upper cover 33 is also made of polycarbonate (PC), glass, acrylic, plastic, etc., and is made of a transparent material. In this way, the upper cover 33 is firmly fixed to the base substrate 31 with the adhesive 32.

 Reference numeral 34 denotes a recess provided in the upper cover 33, and the recess 34 opens toward the base substrate 31. That is, the measurement chamber 36 is formed by the recess 34 and the base substrate 31. Then, the liquid sample 35 flows into and fills the measurement chamber 36 formed in this way.

 Reference numeral 26 denotes a light emitting portion provided below the measurement chamber 36, which is formed of a laser diode. A light receiving unit 28 is provided above the measurement chamber 36 and is formed of a photodetector. The laser beam emitted from the light emitting unit 26 passes through the liquid sample 35 in the measurement chamber 36 and is received by the light receiving unit 28. The properties of the liquid sample 35 are measured from the attenuation (absorbance) of the laser light caused by the laser light passing through the liquid sample 35.

 FIG. 7 shows an overall view of the analysis disk 22 and shows a partially crushed plan view. The disk 22 is composed of a plurality (six in this embodiment) of independent liquid sample analyzers 41. In each of the liquid sample analyzers 41, the chambers and flow paths described with reference to FIGS. 1 to 4 are formed.

 In FIG. 7, reference numeral 42 denotes an inlet for the liquid sample 35 provided in the vicinity of the axis 43. The inlet 42 for the liquid sample 35 is connected to the liquid sample analyzer 41. In addition, the liquid sample analyzing unit 41 causes the liquid sample analyzing unit 41 to generate a centrifugal force in the outer peripheral direction by rotating the disk 22 in the 44 direction. In this embodiment, since each of the six independent liquid sample analyzers 41 is provided, each of the liquid sample analyzers 41 can independently analyze different types of liquid samples 35 at a time. .

(Embodiment 2)
A second embodiment of the present invention will be described. Note that the same reference numerals are assigned to the same components as those in the first embodiment to simplify the description. FIG. 8 is an enlarged view of the main part of the chamber and the flow path formed in the liquid sample analyzer 41 of FIG. 7 described above, and is different from the first embodiment in the following points. That is, as shown in FIG. 8, a plurality of third chambers 8 are provided, and these are connected in parallel to the flow path connecting the first chamber 3 and the second chamber 12. The third chambers are coupled to each other, and the liquid sample can be moved between the chambers. Here, when the third chamber is connected in parallel to the flow path, there is an effect that a large amount of liquid sample can be processed in a short time, and when the third chamber is connected in series to the flow path. Has the effect of improving the effect of the treatment on the liquid sample, and an effective combination can be selected according to the balance between the treatment time of the liquid sample and the treatment effect. Further, the third chambers 8 may exist independently.

 The obstacle 10 that partially obstructs the movement of the liquid sample inside the third chamber 8 has a slope and a groove with respect to the flow of the liquid sample on the surface thereof. To improve the effect of irregularly disturbing the flow of the liquid sample 4. The irregularities such as grooves formed on the surface of the obstacle 10 also have a function of increasing the contact area between the liquid sample 4 and the air in the chamber.

 The obstacle 10 does not need to have both the slope and the groove at the same time, and may be provided with a hole penetrating the obstacle, and may not be fixed to the wall surface of the third chamber 8. However, when the obstacle 10 is not fixed inside the third chamber, the shape of the obstacle 10 is selected from a spherical shape, a cylindrical shape, and a chip shape, or the obstacles 10 having these shapes are mixed and arranged. May be. At that time, it is necessary to have a dimension that does not enter at least the flow path connected to the chamber. Reference numeral 9 denotes an air port provided in the third chamber 8, and one of them is perpendicular to the rotation direction of the disk so as to actively take in the outside air by using the wind pressure generated by the rotation of the disk. It may have a shape projecting in any direction, and a structure in which outside air hitting the projecting portion is taken into the interior from the air port.

 In addition, a reaction reagent 19 that causes an aerobic reaction with an arbitrary component in the liquid sample is disposed inside the first chamber 3 and the flow path 6. The reaction reagent 19 is disposed in the first chamber or the flow path 6. Either one of the flow paths 6 may be used, and a certain arbitrary component constituting the reaction reagent may be separately disposed in the first chamber 3 and the flow path 6. This reagent 19 has at least an oxidase that reacts with the analyte or its secondary substance or its precursor, and in addition, peroxidase and a coloring reagent (as the coloring reagent, 4-aminoantipyrine and 4-aminoantipyrine and oxidative condensation). Selected from the group consisting of a coupling-type coloring reagent consisting of a combination of chromogens that develop color or a leuco-type chromogen), or an electron carrier such as potassium ferricyanide . Further, the reagent 19 may contain an enzyme for decomposing a substance that interferes with the aerobic reaction and a reagent other than the enzyme contained in the liquid sample 4 (for example, ascorbine for decomposing ascorbic acid. Acid oxidase etc.).

 Since it is configured as described above, the liquid sample 4 flowing from the inlet 1 is a reagent 19 that is disposed inside the first chamber 3 and causes an aerobic reaction with the analyte in the liquid sample 4. Is dissolved and moves to the inlet of the liquid sample stopper 5 through the inlet 16. Here, the movement of the liquid sample 4 is stopped at the entrance of the liquid sample stopper 5 by the action of the surface tension.

 Next, when a centrifugal force generated by rotating the disk is applied from the shaft center to the outer periphery of the disk as indicated by an arrow 7, a force is applied to the liquid sample 4 in the first chamber 3 in the direction of the centrifugal force. The surface tension applied to the entrance of the liquid sample stopper 5 is broken, and the liquid sample 4 passes through the liquid sample stopper 5. The liquid sample 4 moves through the flow path 6 until the liquid level reaches the same level as the distance from the axial center to the liquid level of the liquid sample 4 in the first chamber 3.

 Next, when the rotation of the disk is stopped and the centrifugal force is eliminated, the liquid sample 4 passes through the top portion 6a of the reverse “U-shape” and moves through the flow path 6 by the capillary force. At this time, the liquid sample 4 dissolves the reaction reagent 19 disposed inside the flow path 6 and the aerobic reaction with the analyte in the liquid sample 4 is started. Thereafter, the liquid sample 4 branches in parallel by the branch path 20. The liquid sample 4 reaches the respective inlets 18 of the third chamber 8, but since the thickness dimension 15 of the third chamber 8 is larger than the thickness dimension 14 of the flow path 6, the surface of the liquid sample 4 is The liquid sample 4 does not flow into the third chamber 8 due to the tension.

 Next, when the centrifugal force generated by rotating the analytical disk again is applied from the shaft center to the outer periphery of the disk as shown by the arrow 7, the liquid sample 4 passes through the third chamber 8 and the flow path 11. And move to the second chamber 12.

 At this time, in the process in which the liquid sample 4 passes through the third chamber 8, the obstacle 10 inside the third chamber 8 prevents the movement of the liquid sample 4 in the centrifugal force direction, and is provided on the obstacle 10. The liquid sample 4 diffuses widely along the obstacle 10 inside the third chamber 8 due to the inclined surface, the groove, or the like. Further, by taking in the outside air from the air port 9a of the third chamber 8 by utilizing the rotation of the disk at this time, the introduced outside air passes through the inside of the third chamber 8, and the other air port 9b. To the outside of the disc. As a result, the surface area of the liquid sample 4 with respect to the gas is increased inside the third chamber 8 and the outside air is circulated, so that oxygen is efficiently dissolved in the liquid sample 4.

 Then, the liquid sample 4 that has passed through the third chamber 8 continuously undergoes an aerobic reaction inside the second chamber 12 and causes a color development reaction corresponding to the concentration of the analyte in the liquid sample 4. The resulting change is emitted by the laser diode of the light emitting unit 26 described with reference to FIG. 5 and received by the photodetector of the light receiving unit 28 provided on the other side, and the intensity of this light reception (transmittance). Thus, the absorbance of the liquid sample 4 is measured. Note that the measurement method here is not limited to optical measurement, but instead of the color development reaction, electron transfer is caused according to the concentration of the analyte, which is measured electrochemically, and the analyte is analyzed. The concentration of can also be obtained.

 Therefore, since the analytical disk having the third chamber 8 can efficiently tackle oxygen into the liquid sample, even if an aerobic reaction requiring oxygen is caused inside the disk, oxygen deficiency is caused. Good reaction characteristics can be obtained even in a high-concentration analyte that is difficult to cause and consumes a large amount of oxygen.

  The analysis disk according to the present invention and the analysis apparatus using the disk are useful for measurement by the aerobic reaction of the analysis target because the response characteristics of the aerobic reaction are improved.

(A) The top view which shows the principal part of the analysis disk of this invention in Embodiment 1 of this invention (b) Sectional drawing which shows the principal part of the same analysis disk (A) Plan view showing the main part of the analysis disk (b) Cross section showing the main part of the analysis disk (A) Plan view showing the main part of the analysis disk (b) Cross section showing the main part of the analysis disk (A) Plan view showing the main part of the analysis disk (b) Cross section showing the main part of the analysis disk (c) Plan view and cross section showing the main part of the analysis disk Side view of the analyzer of the present invention Sectional drawing which shows the principal part of the analyzer and the disk for analysis Partially crushed plan view of analysis disk of the present invention The top view which shows the principal part of the disk for analysis in Embodiment 2 of this invention The top view which shows the principal part of the disc for analysis in Embodiment 1 of this invention Plan view showing the main part of the same analysis disk

Explanation of symbols

DESCRIPTION OF SYMBOLS 3 1st chamber 4 Liquid sample 5 Liquid sample stopper 6 Flow path 6a Top part of flow path 6 7 Arrow which shows direction of centrifugal force 8 3rd chamber 9 Air port 10 Obstacle 11 Flow path 12 2nd chamber 14 Flow Road thickness dimension 15 Third chamber thickness dimension 17 Obstacle thickness dimension 18 Third chamber inlet

Claims (16)

A first chamber containing a liquid sample provided on the axial center side;
A second chamber provided outside the first chamber with respect to the axis;
A third chamber provided between the first and second chambers;
A first flow path for connecting the first chamber and the third chamber and transferring the liquid sample in the first chamber to the third chamber;
A second flow path for connecting the third chamber and the second chamber and transferring the liquid sample accommodated in the third chamber to the second chamber;
With
The third chamber has a cross-sectional area larger than the cross-sectional areas of the first and second flow paths in a direction perpendicular to the flow of the liquid sample, and is transferred from the first chamber to the third chamber. The liquid sample has a volume larger than the volume of the liquid sample flowing into the inside, and an obstacle for irregularly disturbing the flow of the liquid sample is arranged inside the third chamber. Analysis disc. The height of the third chamber is higher than the height of the flow path, and the obstacle placed in the third chamber has a height higher than the height of the flow path. The disc for analysis according to claim 1, wherein the disc is for analysis. The analysis disk according to claim 1, wherein a plurality of the obstacles are distributed in the third chamber. 4. The analysis disk according to claim 1, wherein the obstacle is erected from the bottom surface of the third chamber. 5. The analysis disk according to claim 1, wherein the obstacle has a rectangular parallelepiped shape. 6. The analytical disk according to claim 1, wherein the obstruction facing the upstream side of the flow of the liquid sample is an inclined surface. 4. The analysis disk according to claim 1, wherein the obstacle is installed separately from a bottom surface or a wall surface of the third chamber. 8. The analysis disk according to claim 7, wherein the obstacle has a shape of a sphere, a cylinder, or a chip, and has an uneven surface or a through hole on the surface. 9. The analysis disk according to claim 1, wherein an air port for introducing air into the chamber or exhausting air in the chamber is provided in the third chamber. The air port has a shape protruding from the outer wall of the disk in a direction perpendicular to the rotating direction of the disk, and is configured to take in the outside air hitting the protruding portion into the chamber inside the disk as a result of rotating the disk. 9. The analytical disk according to 9. A plurality of the third chambers are arranged in parallel, and the liquid sample in the first chamber is branched by the first flow path and transferred to each of the third chambers, and is transferred to the third chamber. 11. The analysis disk according to claim 1, wherein the obtained liquid sample is joined by the second flow path and guided to the second chamber. 11. 11. The analysis disk according to claim 1, wherein a plurality of third chambers are arranged in series, and the third chambers are connected by a flow path. The analysis according to any one of claims 1 to 12, wherein the reagent that reacts with the liquid sample is disposed in the first flow path or the first chamber upstream of the third chamber. Disc. 14. The analytical disk according to claim 13, wherein the reagent causes aerobic reaction. An analysis device to which the analysis disk according to any one of claims 1 to 14 is mounted, wherein a centrifugal force generating means for generating a centrifugal force around an axis of the analysis disk, and the centrifugal force generating means Control means for controlling the strength of the generated centrifugal force,
After the liquid sample is introduced into the first chamber, the liquid sample is brought to just before the third chamber by capillary action of the first flow path while controlling the centrifugal force generated by the centrifugal force generating means by the control means. Transport
Thereafter, a centrifugal force is applied again by the centrifugal force generating means, whereby the liquid sample is guided into the third chamber and an irregular flow is generated in the liquid sample in the third chamber. Is transferred to the second chamber. 16. The method according to claim 15, wherein when the liquid sample passes through the third chamber, oxygen in the gas existing in the third chamber is dissolved in the liquid sample. The analyzer described.

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