JP2009096777A - Hair-growing and nourishing composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a hair-growing and nourishing composition using a hair-growing active ingredient in combination with a material for inhibiting an apoptosis-inducing and promoting protein, and having improved low-temperature stability. <P>SOLUTION: The hair-growing and nourishing composition contains (A) the hair-growing active ingredient (with the proviso that a component (B) is excluded), (B) an inhibitor of the apoptosis-inducing and promoting protein, (C) a glycerol tri-fatty acid ester, (D) a fatty acid ester of a polyoxyethylene glyceryl ether, and (E) ethanol of ≥50 mass%. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a hair-growth nourishing composition excellent in low-temperature stability.

  Many active hair-growth ingredients such as tocopherol acetate and piroctone olamine are easily dissolved in ethanol, and the storage stability can be improved by blending in a composition with a high blend of ethanol. On the other hand, although extracts such as Chinhi have an inhibitory effect on apoptosis-inducing protein (see Patent Document 1: Japanese Patent Laid-Open No. 2006-232828), water is extracted from plant extracts. In many cases, the solvent occupies half or more of the weight, and the other solvent is composed of a solvent such as ethanol or 1,3-butylene glycol. When this extract is blended in a composition with a high ethanol content, for example, sugars, proteins, salts, and the like in the extract may precipitate over time or instantaneously. Based on the above, it is hoped that the hair-growth composition containing a hair-growth active ingredient that is easily dissolved in ethanol and an inhibitor of apoptosis-inducing promotion protein will suppress these precipitation and precipitation and improve low-temperature stability. It was rare.

JP 2006-232828 A JP-A-10-265349 JP-A-8-73325

  The present invention has been made in view of the above circumstances, and in a hair-growth nourishing composition comprising a hair-growth active ingredient that is easily dissolved in ethanol and an inhibitor of apoptosis-inducing promoting protein, it is possible to suppress these precipitation and precipitation, and to stabilize at low temperature The purpose is to improve the performance.

  As a result of intensive studies to achieve the above object, the present inventor has developed a hair growth nourishing composition comprising (A) an active ingredient for hair restoration that is easily dissolved in ethanol, and (B) an inhibitor of a protein that promotes apoptosis induction. C) Tri-fatty acid glyceryl, (D) polyoxyethylene glyceryl ether fatty acid ester and (E) ethanol are blended, and (E) the content of ethanol is 50% by mass or more in the composition. It was found that both the component and the inhibitor of the (B) apoptosis induction promoting protein were solubilized in the composition, and the low-temperature stability was improved.

Therefore, the present invention
[1]. (A) Hair-growth active ingredient (excluding component (B)), (B) inhibitor of apoptosis-inducing protein, (C) tri-fatty acid glyceryl, (D) polyoxyethylene glyceryl ether fatty acid ester, and (E) A hair restorer composition containing 50% by mass or more of ethanol,
[2]. The component (A) is selected from the group consisting of tocopherol acetate, piroctone olamine, β-glycyrrhetinic acid, isopropylmethylphenol, pentadecanoic acid monoglyceride, minoxidil, carpronium chloride, forskolin, swellthiamarin, adenosine and t-flavanone. The hair-growth nourishing composition according to [1], which is one or more kinds of hair-growth active ingredients,
[3]. (B) The hair restorer composition according to [1] or [2], wherein the component is an extract of a plant or seaweed selected from the group consisting of phytocollage, Asaku, Okinawa mozuku and Dulse.

  According to the present invention, it is possible to improve the low-temperature stability of a hair-growth hair nourishing composition comprising a combination of (A) an effective hair-growth component (excluding the component (B)) and (B) an inhibitor of apoptosis induction promoting protein. Can do.

  The hair-growth nourishing composition of the present invention comprises (A) a hair-growth active ingredient (except for component (B)), (B) an inhibitor of apoptosis-inducing protein, (C) tri-fatty acid glyceryl, (D) polyoxyethylene A hair-growth nourishing composition containing glyceryl ether fatty acid ester and (E) 50% by mass or more of ethanol.

(A) Hair-growth active ingredient As a hair-growth active ingredient of this invention, a well-known component is mentioned as a hair-growth active ingredient. However, it is a component excluding the component (B) described later. As the hair-growth active ingredient, those that are easily dissolved in ethanol are preferable. Examples of hair-growth active ingredients that are easily dissolved in ethanol include tocopherol acetate, piroctone olamine, β-glycyrrhetinic acid, isopropylmethylphenol, pentadecanoic acid monoglyceride, minoxidil, carpronium chloride, forskolin, swellthiamarin, adenosine and t-flavanone Can be used alone or in combination of two or more. Among these, pentadecanoic acid monoglyceride, minoxidil, carpronium chloride, tocopherol acetate, piroctone olamine, adenosine, and t-flavanone are preferable.

  (A) Content in the hair-growth nourishing composition of the hair-growth active ingredient is usually 0.001 to 10.0% by mass, preferably 0.01 to 7.0% by mass, and 0.05 to 3.0% by mass. % Is more preferable. When the content is less than 0.001% by mass, a sufficient hair-growth effect may not be obtained. When the content exceeds 10.0% by mass, the hair-growth active ingredient may be precipitated at a low temperature.

(B) Inhibitor of apoptosis induction promoting protein By blending the inhibitor of apoptosis induction promoting protein of the present invention, the hair loss factor can be suppressed, and (A) in combination with an active ingredient for hair growth, A synergistic effect can be obtained. (B) A component can be used individually by 1 type or in combination of 2 or more types.

  The inhibitor of apoptosis induction promoting protein in the present invention refers to a substance that inhibits the induction of apoptosis by an apoptosis induction promoting protein (Neurotrophin-4, hereinafter abbreviated as “NT-4”). Specifically, it refers to a substance that suppresses 50% or more compared to the addition of NT-4 alone, based on test examples described later.

  Examples of the inhibitory substances include chinhi, Kusetsushobu, liquor blossom (shuyakuka), Japanese astragalus, phytocollage (derived from pea (soybean)), deuterium (juyuboboku), tachibana ( Extract of plant or seaweed selected from the group consisting of cypress, Guangxi, Asaku, Echonia, Darus, Davilia, Okakusa, Tsutsuregusa, Tochaka, Calophyllus, Okinawa Mozuku, Ginnan, Dragusa, Fruceraria, Willow Mok and Mozuku ). Among these, an extract of phytocollage, Asaku, Okinawa mozuku or duls is preferable.

  The plant or seaweed used for the extraction of the above plant or seaweed extract is not particularly limited in its application part, but from the viewpoint of exerting its effectiveness, asaku is made of wood, echronia, dulls, okusa, tsutsuregusa, tochaka, It is preferable to use the whole plant or seaweed for Calophyllus, Okinawa Mozuku, Ginnan Sou, Dragusa, Fruceraria, Willow Mok, and Mozuku. The “wood” means the woody part of the stem of a woody plant.

  As the extract, a commercially available product or one obtained by a known extraction method can be used. Examples of the solvent used in the extraction method (hereinafter referred to as “extraction solvent”) include water; alcohols such as methanol, ethanol, propanol, and butanol; polyhydric alcohols such as propylene glycol and butylene glycol, and the like. Can be used singly or in appropriate combination of two or more.

  Various conditions in the extraction method are not particularly limited, but the ratio of the extraction raw material to the extraction solvent is usually in the range of extraction raw material: extraction solvent = 1: 2 to 1:50 by mass ratio. preferable. The extraction temperature is preferably in the range of 5 to 80 ° C., and is preferably performed by immersing or stirring in the extraction solvent for 1 hour to 1 week. The extraction pH is not particularly limited unless it is extremely acidic or alkaline.

  When the extraction solvent is a non-toxic solvent such as water, ethanol, water / ethanol (hydrous ethanol) or the like, the extract may be used as it is as a hair growth agent or as a diluent. Moreover, the said extract may be used as a concentrated extract, and may be made into a dry powder by freeze-drying or the like, or may be prepared in a paste form. When other solvents are used, it is preferable to dilute the dried portion with a non-toxic solvent after distilling off the solvent.

  The content of the component (B) in the hair-growth agent composition is usually 0.01 to 10% by mass, preferably 0.1 to 5% by mass. If it is less than 0.01% by mass, the effect of suppressing apoptosis cannot be exhibited, and if it exceeds 10% by mass, it becomes uneconomical and the low-temperature stability may be deteriorated.

(C) Trifatty acid glyceryl As trifatty acid glyceryl, glyceryl tri-2-ethylhexanoate, tri (caprylic / capric acid) glyceryl, tri (caprylic acid / capric acid / stearic acid) glyceryl, glyceryl triisostearate, glyceryl trioctanoate The tri-fatty acid glyceryl etc. which have three C6-C20 fatty acids, such as these, are mentioned, It can use individually by 1 type or in combination of 2 or more types. Among these, glyceryl tri-2-ethylhexanoate is preferable.

  (C) The content of tri-fatty acid glyceryl in the hair-growth nourishing composition is usually 0.05 to 20% by mass, preferably 0.1 to 10% by mass. If it is less than 0.05% by mass, stabilization at a low temperature may be difficult, and if it exceeds 20% by mass, the feeling of use (stickiness) may be deteriorated.

(D) Polyoxyethylene glyceryl ether fatty acid ester As polyoxyethylene glyceryl ether fatty acid ester, polyethylene isostearate (3) glyceryl (may be referred to as PEG-3 glyceryl isostearate), polyethylene isostearate (5) glyceryl (PEG-5 glyceryl isostearate, polyethylene (10) glyceryl isostearate (PEG-10 glyceryl isostearate), polyethylene (15) glyceryl isostearate (PEG-15 glyceryl isostearate), polyethylene isostearate (20) glyceryl (isostearic acid) PEG-20 glyceryl), polyethylene isostearate (30) glyceryl (PEG-30 glyceryl isostearate) , Polyethylene (40) glyceryl isostearate (PEG-40 glyceryl isostearate), polyethylene (50) glyceryl isostearate (PEG-50 glyceryl isostearate), polyethylene isostearate (60) glyceryl (PEG-60 glyceryl isostearate), etc. Polyoxyethylene glyceryl isostearate, polyethylene diisostearate (10) glyceryl (PEG-10 glyceryl diisostearate), polyethylene diisostearate (20) glyceryl (PEG-20 glyceryl diisostearate), polyethylene diisostearate (30) glyceryl (PEG diisostearate) -30 glyceryl) polyethylene glyceryl diisostearate, triisostearic acid Liethylene (3) glyceryl (PEG-3 glyceryl triisostearate), polyethylene triisostearate (5) glyceryl (PEG-5 glyceryl triisostearate), polyethylene triisostearate (10) glyceryl (PEG-10 glyceryl triisostearate) , Polyethylene triisostearate (15) glyceryl (PEG-15 glyceryl triisostearate), polyethylene triisostearate (20) glyceryl (PEG-20 glyceryl triisostearate), polyethylene triisostearate (30) glyceryl (PEG triisostearate) -30 glyceryl), polyethylene triisostearate (40) glyceryl (PEG-40 glyceryl triisostearate) and the like Polyoxyethylene stearate glyceryl, and the like. These can be used individually by 1 type or in combination of 2 or more types. Among these, polyethylene isostearate (15) glyceryl (PEG-15 glyceryl isostearate), polyethylene diisostearate (30) glyceryl (PEG-30 glyceryl diisostearate), polyethylene triisostearate (30) glyceryl (PEG-30 triisostearate) Glyceryl) is preferred.

  (D) Content in the hair-restoring agent composition of polyoxyethylene glyceryl ether fatty acid ester is 0.05-20 mass% normally, and 0.1-10 mass% is preferable. If it is less than 0.05% by mass, stabilization at low temperatures may be difficult, and if it exceeds 20% by mass, the feeling of use (stickiness) may be deteriorated.

(E) Ethanol The (E) ethanol content in the hair-growth nourishing composition of the present invention is 50% by mass or more, preferably 50 to 85% by mass, more preferably 50 to 70% by mass. (E) If ethanol content is less than 50 mass%, (B) component will melt | dissolve easily, but (A) hair-growth active ingredient precipitates.

  The hair growth agent composition of the present invention can be blended with any one component alone or in combination of two or more. Examples of such components include hair-growth active ingredients other than the component (A), anti-androgen hormones, moisturizers, vitamins other than the component (A), amino acids, celluloses, surfactants, fats and oils, and liquids. An ester oil (25 ° C.), a polymer resin, a coloring material, a fragrance, an ultraviolet absorber, and the like can be blended.

  The dosage form of the hair restorer composition is not particularly limited, and can be used externally in the form of a uniform solution, lotion, gel or the like according to a conventional method. Further, the hair-restoring agent composition of the present invention can take the form of an aerosol spray, in which case, in addition to the above components, lower alcohols such as n-propyl alcohol or isopropyl alcohol, butane, propane, isobutane, liquefaction. Combustible gases such as petroleum gas and dimethyl ether, and compressed gases such as nitrogen gas, oxygen gas, carbon dioxide gas, and nitrous oxide gas can be contained. Moreover, the hair-growth nourishing agent composition of this invention can mix the said essential component and arbitrary components, and can be obtained based on a conventional method.

  The present invention relates to a hair-growth nourishing composition containing (A) an effective hair-growth component (excluding the component (B)) and (B) an inhibitor of apoptosis-inducing promoting protein, (C) tri-fatty acid glyceryl, ( D) Polyoxyethylene glyceryl ether fatty acid ester and (E) ethanol are blended, and (E) the content of ethanol is 50% by mass or more in the composition. Provide a method.

  EXAMPLES Hereinafter, although a preparation example, a test example, an Example, and a comparative example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to the following Example. In the following examples, unless otherwise specified, “%” in the composition represents mass%.

[Test Examples 1 to 21]
The plants shown in Table 1 as test samples were extracted with 70% ethanol and seaweed with water, and the resulting extract was dried to obtain a solid. This solid was dissolved in ethanol to a concentration of 100 mg / mL. The extract thus prepared was added to keratinocytes previously cultured at 20,000 cells / well so that the final concentration was 100 μg / mL. At the same time, apoptosis induction promoting protein (Neurotrophin-4, hereinafter referred to as “NT-4”) was added to the well so that the final concentration was 1 ng / mL. After 24 hours, the apoptosis induction rate was measured using the TUNEL method. The apoptosis induction rate was determined by staining the cells causing apoptosis using a TUNEL staining kit “TACS _™ 2 TdT DAB” (manufactured by TREVIGEN) and determining the ratio thereof. As a result of the apoptosis induction inhibition test, the induction inhibition rate (%) was calculated with the addition of NT-4 alone as the apoptosis induction rate of 100%, and the results were shown by the following criteria.
<Standard>
A: Suppressed by 80% or more compared to the case of adding only NT-4 ○: Suppressed by 50% or more and less than 80% compared to the case of adding only NT-4 ×: Suppressed by less than 50% compared to the case of adding only NT-4

  Table 1 shows the results of the apoptosis induction suppression test. As shown in Table 1, the extract was found to suppress the induction of apoptosis. In particular, it was revealed that apoptosis induction was excellent in scab, asaku, echronia, dallus, and geek. Therefore, it was confirmed that the extract was an inhibitor of apoptosis induction promoting protein.

[Comparative Test Example 1]
Ethanol was added to keratinocytes previously cultured at 20,000 cells / well to a final concentration of 0.1%. At the same time, apoptosis induction promoting protein (NT-4) was added to the well so that the final concentration was 1 ng / mL. Apoptosis induction rate was determined in the same manner as in the above test example. As a result, the action of the apoptosis induction promoting protein was not suppressed, and apoptosis was induced.

[Examples 1-48, Comparative Examples 1-4]
A hair growth nourishing composition having the composition shown in Tables 3 to 23 was prepared, and stability evaluation was performed by the following method. The results are listed in the table. For each extract, those obtained in Preparation Examples 1 to 3 were used. In the following examples, unless otherwise specified, “%” of the composition is indicated by mass%, and the blending amount of ethanol is indicated by a pure content.

<Low temperature stability>
About 20 mL of a sample to be tested was placed in a 50 mL transparent glass bottle (however, aerosol was 50 g in a 100 mL transparent pressure-resistant bottle) and stored in a thermostatic bath at −10 ° C., and then crystal precipitation was visually observed. The number of days subtracted one day from the date of precipitation was defined as the low-temperature stabilization days, and evaluation was performed up to 60 days.

About the hair restoring agent of Examples 1, 28, 30, 31, and 32, the hair restoring effect was evaluated by the following method.
Using male C57BL / 6 mice (7 weeks old), Ogawa et al. (See Fragrance Journal, Vol. 17, No. 5, p20-29, 1989.), and JP-A-2006-256994. The method was used as a reference.
The hair on the back of male C57BL / 6 mice (7 weeks old) was removed to a size of about 2 × 4 cm with an electric clipper and an electric shaver to induce the hair growth period. After depilation, about 30 mg of dihydrotestosterone (DHT), one of the causative substances of male pattern hair loss, was administered subcutaneously. As a comparative control in which DHT was not administered, a sham operation group was set (DHT non-administration group). Each sample (test substance) administration group, ethanol 70% by mass administration group, and DHT non-administration group use 8 mice, and from the next day of hair removal and DHT subcutaneous administration, once per day 100 μL, 5 times a week Application was performed. 18 days after hair removal, the hair growth area where hair regeneration started on the hair removal part was visually observed, and the hair growth score was evaluated with 0 to 10 points. A result is shown by the score total of 8 animals, and a score average value.
<Evaluation criteria for hair growth score>
Score 0 point: No hair growth Score 1 point: The ratio of hair growth area is less than 0-10% Score 2: Point of hair growth area is less than 10-20% Score 3: Point ratio of hair growth area However, 20 to less than 30% score 4 points: the proportion of hair growth area is less than 30 to 40% score 5 points: the proportion of hair growth area is 40 to less than 50% score 6 points: the proportion of hair growth area is 50 to less than 60% score 7 points: hair growth area ratio is 60 to less than 70% score 8 points: hair growth area ratio 70 to less than 80% score 9 points: hair growth area ratio 80 to Less than 90% score 10 points: the ratio of hair growth area is 90-100%

[Preparation Example 1]
Phytocollagen extract To a 20% ethanol solution extract of natto obtained by fermenting soybean seeds with natto bacteria, 99% ethanol was added to precipitate massive mucus. A phytocollagen extract was obtained by dispersing in a 10% ethanol solution so that the mass was 0.3%.

[Preparation Example 2]
Asaku extract After asaku was crushed, it was shaken with 50% ethanol all day and night, and the resulting extract was dried with an evaporator to obtain a solid. This solid was dispersed in a 20% ethanol solution so as to be 1% to obtain an Asaku extract.

[Preparation Example 3]
Dulse extract, Okinawa mozuku extract (seaweed)
Dulse and Okinawa mozuku were crushed and then extracted with water for 2 hours, and the resulting extract was dried by freeze drying to obtain a solid. This solid was dispersed in a 20% ethanol solution so as to be 1% to obtain a dull extract and Okinawa mozuku extract.

Claims (3)

  (A) Hair-growth active ingredient (excluding component (B)), (B) inhibitor of apoptosis-inducing protein, (C) tri-fatty acid glyceryl, (D) polyoxyethylene glyceryl ether fatty acid ester, and (E) A hair restorer composition containing 50% by mass or more of ethanol.   The component (A) is selected from the group consisting of tocopherol acetate, piroctone olamine, β-glycyrrhetinic acid, isopropylmethylphenol, pentadecanoic acid monoglyceride, minoxidil, carpronium chloride, forskolin, swellthiamarin, adenosine and t-flavanone. The hair-growth hair nourishing composition according to claim 1, which is one or more hair-growth active ingredients.   (B) The hair-growth nourishing composition according to claim 1 or 2, wherein the component is an extract of a plant or seaweed selected from the group consisting of phytocollage, Asaku, Okinawa mozuku and Dulse.