JP2009035510A - Dentifrice composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a dentifrice composition comprising dextranase, having an excellent feeling of use and excellent removing effects on dental plaque and effective for preventing dental caries. <P>SOLUTION: The dentifrice composition is characterized in that a dentifrice composition comprising the dextranase is formulated with (A) 0.5-2 mass% of a polyglycerol fatty acid ester, (B) 0.5-2 mass% of a water-soluble pyrophosphate and (C) 0.5-1.5 mass% of an alkyl sulfate and the mass ratio of the ingredient (A)/the ingredient (B) is 0.5-4. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a dentifrice composition that is excellent in feeling of use and effective in preventing dental caries that is excellent in plaque removal effect.

  The existence of dental plaque is known as one of the main causes of caries. This plaque is mainly composed of bacteria and bacteria-derived glucan. Caries are generated by the decalcification of the teeth by the acid produced by bacteria in the plaque. Therefore, the removal of dental plaque is very effective in preventing caries.

  The glucan in this plaque is a complex mixture of dextran having an α-1,6 bond and mutan having an α-1,3 bond. Of these, dextranase is an enzyme that degrades α-1,6 bonds, and has been conventionally known to have caries-preventing effects such as plaque formation-inhibiting effects (see Patent Document 1).

  Patent Document 2 proposes that the use of dextranase and mutanase in combination and the inclusion of a specific nonionic surfactant can maintain the stability of dextranase and maintain the plaque removal effect. However, the combined use of mutanase is thought to increase the plaque removal effect compared to the case of dextranase alone, but the feeling of use (flavor) as the total amount of enzyme is increased to increase the plaque removal effect. Tended to be worse.

  Patent Document 3 proposes a technique in which fine calcium carbonate and alkyroyl sarcosinate are used in combination with dextranase. However, when a substance that separates calcium ions such as fine calcium carbonate is blended, Ingredients that generate insoluble salts, such as sodium fluoride, which is a fluoride ion supplying substance that is recognized to have a caries prevention effect, are limited, and the ingredients that can be used are limited, making them difficult to commercialize.

  Accordingly, it has been desired to develop a dextranase-containing dentifrice composition that exhibits a high plaque removal effect without significantly increasing the amount of an enzyme such as dextranase.

Japanese Patent No. 782154 JP-A-8-12543 Japanese Patent Laid-Open No. 11-35438 JP-A-6-192060 JP 9-175966 A Japanese Patent Laid-Open No. 10-182389 JP 2002-68947 A JP 2005-187329 A JP 2004-187329 A JP 2006-117574 A JP 2005-41787 A JP 2007-145740 A

  The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a dextranase-containing dentifrice composition that is excellent in use feeling and effective in preventing dental caries, and has an excellent effect of removing plaque.

  As a result of intensive studies to achieve the above object, the present inventors have determined that the dentifrice composition containing dextranase has a polyglycerin fatty acid ester content of 0.5-2% (mass%, the same shall apply hereinafter). , 0.5 to 2% of water-soluble pyrophosphate and 0.5 to 1.5% of alkyl sulfate, and the mass ratio of polyglycerol fatty acid ester / water-soluble pyrophosphate to 0.5 It turned out that it is surprisingly excellent in the usability by using -4, and the outstanding plaque removal effect is exhibited.

  In addition, water-soluble pyrophosphate is well-known as a compounding component of an oral composition, has a plaque formation inhibitory effect (refer patent document 4), and is combined with orthophosphoric acid, sorbitol, and calcium in dental plaque. It is known that the acid neutralization effect is exhibited (see Patent Document 5). It has also been proposed to be used as a pellicle stain removing component on the tooth surface (see Patent Documents 6 and 7) and to be used in combination with particles in order to increase the effect of removing the pellicle on the tooth surface (see Patent Document 8).

  Polyglycerin fatty acid ester is known as a compounding ingredient for oral compositions, and can be used in combination with dextranase, mutanase and nonionic surfactants to maintain dextranase stability and maintain plaque removal effect. Has been proposed (see Patent Document 1).

  Furthermore, Patent Document 8 proposes that berberine can be stabilized by combining water-soluble pyrophosphate and polyglycerin fatty acid ester. Patent Document 9 discloses a combination formulation of polyglycerin fatty acid ester, inorganic acid and / or organic acid. Further, Patent Document 10 proposes that a biofilm formation inhibitory effect can be obtained by combining a polyglycerin fatty acid ester and polyphosphoric acid or a salt thereof with a cationic fungicide to enhance the cationic fungicide. .

  Alkyl sulfate is mainly blended as a foaming agent in a dentifrice composition, but is known to destabilize enzymes such as glucanase. Patent Documents 11 and 12 disclose polyoxygens for enzyme stabilization. It is disclosed that a nonionic surfactant such as ethylene alkyl ether or polyoxyethylene hydrogenated castor oil is used in combination.

  On the other hand, the present inventors selectively blend polyglycerin fatty acid ester, water-soluble pyrophosphate, and alkyl sulfate in a specific ratio in a dentifrice composition containing dextranase. Therefore, without increasing the total amount of enzyme in the composition, dextranase can be blended in the usual amount of dentifrice composition to achieve an excellent plaque removal effect. It is possible to mix fluoride ion supply substances such as sodium fluoride with no restrictions on the ingredients, and there is no irritation or stickiness when brushing teeth. The present inventors have found that an effective dentifrice composition can be obtained. As will be apparent from the examples described later, when any of the essential requirements of the present invention is not satisfied, it is impossible to combine an excellent plaque removing effect and a feeling of use, and the object of the present invention is not achieved.

  Accordingly, the present invention provides a dentifrice composition containing dextranase in which (A) polyglycerol fatty acid ester is 0.5-2%, (B) water-soluble pyrophosphate is 0.5-2%, (C) A dentifrice composition comprising 0.5 to 1.5% of alkyl sulfate and a mass ratio of (A) component / (B) component of 0.5 to 4 is provided. To do.

  The dentifrice composition of the present invention has an excellent feeling of use and has an excellent plaque removing effect, and is useful for caries prevention.

  Hereinafter, the present invention will be described in more detail. The dentifrice composition of the present invention is prepared as a dentifrice such as a toothpaste, a liquid dentifrice, a liquid dentifrice, particularly a toothpaste, and contains dextranase, polyglycerin fatty acid ester, water-soluble pyrophosphate, and alkyl sulfate as essential ingredients. contains.

  In the present invention, as the dextranase, a dextranase obtained by a known method from a known dextranase-producing bacterium belonging to the genus Ketomium, Penicillium, Aspergillus, Spicaria, Lactobacillus, Servibrio, etc. Although it can be used, dextranase produced from other microorganisms can also be used, and products manufactured by Sankyo Co., Ltd. can be used as commercial products.

  In the present invention, the amount of dextranase is 2 to 200 units / g in the composition, and 10 to 50 units / g is particularly preferable. When the amount is less than 2 units / g, a sufficient plaque removing effect cannot be obtained, and when it exceeds 200 units / g, the stability of the enzyme may decrease. Here, 1 unit of dextranase is the amount of dextranase that produces free reducing sugar corresponding to 1 μmol of glucose per minute when the reaction is carried out using dextran as a substrate. Usually, when a 13,000 unit / g thing is used, a compounding quantity will be 0.0154 to 1.54% of the whole composition.

  In the present invention, (A) polyglycerin fatty acid ester is blended. As the polyglycerin fatty acid ester, those having an alkyl group having 12 to 16 carbon atoms, particularly 12 to 14 carbon atoms are preferable in terms of the stability of the dentifrice and the plaque removing effect. If the alkyl group has less than 12 carbon atoms, the plaque removal effect may not be sufficiently exerted, and if it exceeds 16, the stability of the preparation such as liquid separation may be inferior.

  In addition, the polyglycerol fatty acid ester preferably has an average addition mole number of glycerol of 4 to 10 mol, particularly 6 to 10 mol from the viewpoint of the stability of the dentifrice (liquid separation) and the plaque removing effect. If the number of added moles is less than 4 moles, composition stability may be deteriorated, for example, liquid separation may occur, and if it exceeds 10 moles, the effects of the present invention may not be sufficiently obtained.

  Specific examples of such polyglyceryl fatty acid esters include hexaglyceryl monolaurate, hexaglyceryl monomyristate, decaglyceryl monolaurate, decaglyceryl monomyristate, hexaglyceryl monocetylate, hexaglyceryl monocetylate, and the like. Decaglyceryl monolaurate and decaglyceryl monomyristate are preferred.

  Commercially available products can be used as the polyglycerin fatty acid ester, for example, NIKKOL Hexaglyn 1-L (hexaglyceryl monolaurate), NIKKOL Hexaglyn 1-M (hexaglyceryl monomyristate), NIKOL Decaglyn 1 manufactured by Nikko Chemicals Co., Ltd. -L (decaglyceryl monolaurate), NIKKOL Decaglyn 1-M (decaglyceryl monomyristate) and the like.

  The compounding quantity of polyglycerol fatty acid ester is 0.5 to 2% of the whole composition, Preferably it is 1.0 to 2%. If the blending amount is less than 0.5%, a sufficient plaque removing effect is not exhibited, and if it exceeds 2%, the feeling of use becomes worse, for example, the sticky feeling becomes stronger, and the stability of the preparation may be worsened.

The water-soluble pyrophosphate of component (B) is represented by the chemical formula M ₄ · P ₂ O ₇ (wherein M is a hydrogen ion or an alkali metal ion such as sodium or potassium), and dehydration of phosphoric acid. Condensed. Specific examples of such water-soluble pyrophosphates include tetrasodium pyrophosphate, disodium dihydrogen pyrophosphate, disodium 2 potassium pyrophosphate, and 4 potassium pyrophosphate. preferable. Specifically, those manufactured by Taihei Chemical Co., Ltd. or Nippon Chemical Industry Co., Ltd. can be used.

  The compounding amount of the water-soluble pyrophosphate is 0.5 to 2% of the whole composition, and particularly preferably 0.8 to 1.5%. If the blending amount is less than 0.5%, the effect of removing plaque is not sufficiently exhibited.

  In the composition of the present invention, the mass ratio of (A) polyglycerin fatty acid ester / (B) water-soluble pyrophosphate is 0.5 to 4, and particularly 1 to 4 is excellent in usability and plaque removal. It is suitable for satisfying the effect. If the mass ratio is less than 0.5, the feeling of use becomes worse, for example, stickiness when brushing becomes strong, and if it exceeds 4, irritation occurs in the oral cavity and the effect of removing plaque becomes worse.

  (C) As the alkyl sulfate, those having 8 to 18, particularly 12 to 14 carbon atoms of the alkyl group are suitable, and alkali metal salts such as sodium salts and potassium salts are used. If the alkyl group has less than 8 carbon atoms, irritation may be felt during use, and the taste may deteriorate, and if it exceeds 18, the stability of the preparation such as liquid separation may be poor.

  Specific examples of the alkyl sulfate include potassium lauryl sulfate, sodium lauryl sulfate, triethanolamine lauryl sulfate, sodium myristyl sulfate, sodium cetyl sulfate and the like, and particularly sodium lauryl sulfate (Toho) from the viewpoint of solubility and usability. Chemical Industries, Ltd., Nikko Chemicals, etc.) are preferred.

  The compounding amount of the alkyl sulfate is 0.5 to 1.5%, preferably 0.8 to 1.2% of the entire composition. If the blending amount is less than 0.5%, the effect of removing plaque will be reduced, or foaming will be weak and inferior in brushing comfort. There is.

  In the dentifrice composition of the present invention, in addition to the above-described components, various other components that are usually used in dentifrice compositions can be blended as optional components as long as the effects of the present invention are not impaired. Examples of other components that can be blended include binders, surfactants, abrasives, thickeners, sweeteners, fragrances, pH adjusters, active ingredients or medicinal ingredients, and preservatives.

  Examples of the binder include carrageenan, methylcellulose, sodium hydroxyethylcellulose, sodium alginate, xanthan gum, tragacanth gum, karaya gum, arabiya gum, locust bean gum, polyvinyl alcohol, sodium polyacrylate, carboxyvinyl polymer, polyvinylpyrrolidone, carbopol, beegum and the like. is there. The compounding quantity of a binder is 0.3 to 2% of the whole composition normally.

As surfactant, in addition to (C) component alkyl sulfate, (A) component polyglycerol fatty acid ester, other anionic surfactants, cationic surfactants, other nonionic surfactants Can be blended.
As the anionic surfactant, in addition to the alkyl sulfate salt of component (C), other anionic surfactants such as sodium N-lauroyl sarcosinate, sodium N-myristol sarcosinate, dodecylbenzene sulfonic acid Sodium, hydrogenated coconut fatty acid monoglyceride sodium monosulfate, sodium lauryl sulfoacetate, sodium α-olefin sulfonate, N-acyl glutamate such as sodium N-palmitoyl glutamate, N- such as sodium N-methyl-N-acyl taurate An acyl taurate etc. can be mix | blended. Examples of the cationic surfactant include alkyl ammonium and alkyl benzyl ammonium salts.

  As the nonionic surfactant, in addition to the polyglycerin fatty acid ester (A), other nonionic surfactants such as sugar fatty acid esters such as sucrose fatty acid ester and maltose fatty acid ester, maltitol fatty acid ester, Sugar alcohol fatty acid ester such as lactol fatty acid ester, alkylol amide, polyoxyethylene sorbitan fatty acid ester such as polyoxyethylene sorbitan monostearate, polyoxyethylene fatty acid ester such as polyoxyethylene hydrogenated castor oil, lauric acid mono or diethanolamide Fatty acid diethanolamide, sorbitan fatty acid ester, polyoxyethylene higher alcohol ether, polyoxyethylene polyoxypropylene copolymer, polyoxyethylene polyoxypropylene Fatty acid esters, Pluronic, and the like.

  The blending amount of the surfactant as the optional component is preferably 0.1 to 2%, particularly preferably 0.1 to 1.5% of the entire composition.

  As abrasives, dicalcium phosphate dihydrate and anhydrous salts, calcium phosphate compounds such as primary calcium phosphate, tertiary calcium phosphate and calcium pyrophosphate, silica compounds such as precipitated silica and aluminosilicate, calcium carbonate, water One or more of calcium oxide, aluminum hydroxide, aluminum silicate, tribasic magnesium phosphate, magnesium carbonate, calcium sulfate, bentonite and the like can be blended. The blending amount of the abrasive is usually 0 to 50%, particularly 2 to 40% of the whole composition.

  As the thickener, one kind of polyhydric alcohol such as glycerin, sorbit, propylene glycol, polyethylene glycol having a weight average molecular weight of 200 to 6,000, ethylene glycol, 1,3-butylene glycol, reduced starch saccharified product, etc. is used alone. Or it can mix | blend in combination of 2 or more types. The amount is usually 5 to 70% based on the total amount of the composition.

  Examples of sweetening agents include saccharin sodium, stevioside, stevia extract, paramethoxycinnamic aldehyde, neohesperidyl hydrochalcone, perilartin, glycyrrhizin, thaumatin, asparatylphenylalanine methyl ester, and the like. The compounding quantity of a sweetener is 0.01 to 5% of the whole composition normally.

  As fragrances, menthol, anethole, carvone, eugenol, limonene, n-decyl alcohol, citronellol, α-terpineol, citronellyl acetate, cineol, linalool, ethyl linalool, crocodile, thymol, spearmint oil, peppermint oil, lemon oil, orange Oil, sage oil, rosemary oil, cinnamon oil, pimento oil, cinnamon oil, perilla oil, winter green oil, clove oil or eucalyptus oil. The compounding quantity to the composition of a fragrance | flavor can be 0.001-2%.

  Examples of the pH adjuster include organic acids such as citric acid, lactic acid, malic acid and salts thereof, and inorganic compounds such as hydrochloric acid, sodium hydroxide, potassium hydroxide, disodium hydrogen phosphate, sodium dihydrogen phosphate, and the like. .

  In addition to dextranase, which is an essential ingredient, medicinal ingredients or active ingredients include enzymes other than dextranase such as amylase, protease, and mutanase, tranexamic acid, epsilon aminocaproic acid, aluminum chlorohydroxyallantoin, azulene, glycyrrhizinate , Anti-inflammatory agents such as glycyrrhetinic acid, sodium chloride and vitamins, copper chlorophyll, copper gluconate, cetylpyridinium chloride, benzalkonium chloride, triclosan, hinokitiol, lysozyme chloride and other polyphosphoric acids other than water-soluble pyrophosphate Tartar preventive agents such as acid salts, tobacco spider removers such as polyvinylpyrrolidone, amino acids such as glycine and proline can be added.

  In addition, as an optional component, inorganic compounds such as calcium hydrogen phosphate, zeolite, hydroxyapatite, granulated products thereof, granulated products of organic powders such as crystalline cellulose, agar, gelatin, starch, glucomannan, etc. Natural polymers, synthetic polymers such as polyvinyl acetate, acrylic, polyurethane, polyester, polyamide, nylon, polyvinyl chloride and their copolymers, carnauba wax, rosin, rice wax, microcrystalline wax, beeswax, paraffin wax Waxes such as cetanol, stearyl alcohol and higher alcohols, polyisobutylene, polybutadiene, urethane, silicone, natural rubber and other latex rubbers obtained by crosslinking, polymerization, molding, etc., or these raw materials Mixed Can be used are ones and lame film. Lame films include organic resin laminated film powder, laminated film powder in which a vapor deposition layer such as aluminum is introduced into the organic resin laminated film, specifically, polyethylene terephthalate / polymethyl methacrylate laminated film powder, polyethylene terephthalate・ Aluminum / epoxy laminated powder or the like can be blended within a range not impeding the effects of the present invention.

  EXAMPLES Hereinafter, although an Example and a comparative example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to the following Example. In the following examples, the blending amount is mass%.

[Examples 1-8, Comparative Examples 1-14]
Toothpaste compositions having the compositions shown in Tables 1 and 2 were prepared by a conventional method, and the plaque removal effect and feeling of use were evaluated by the following methods. The results are shown in Tables 1 and 2. The raw materials used are as shown below.

<Measurement of plaque removal effect>
Inoculate Streptococcus sorbinus 6715 in 4 ml of tryptic soy broth medium (Tryptic Soy Broth medium, manufactured by Becton Dickinson) containing 1% sucrose, and incubate overnight at 37 ° C. Plaque was formed on the wall. After washing 3 times with distilled water, 5 ml of a sample solution obtained by diluting the prepared dentifrice 10-fold with distilled water was added and allowed to stand at 37 ° C. for 5 minutes. The sample solution was removed, washed 3 times with distilled water, and 4 ml of distilled water was added.
The plaque remaining on the test tube wall was peeled off with a sonicator, and the turbidity of the dispersed solution was measured with an absorptiometer. The degree of effect was calculated by comparing the removal rate with a removal rate of 0% for samples using distilled water instead of the sample solution, and a removal rate of 100% for those measured with distilled water without performing plaque formation treatment. .

<Evaluation of feeling of use>
Ten expert panelists extruded 1 cm of the test dentifrice composition filled in the laminate tube onto the toothbrush, brushed it for 2 minutes in the same manner as usual, and evaluated the test dentifrice composition according to Evaluation Criteria-1 shown below. The average score of 10 people was evaluated by Evaluation Criteria-2.
Evaluation criteria-1
5 points: I feel no irritation and stickiness while brushing my teeth and feel good 4 points: I feel some irritation and stickiness while brushing my teeth, but I feel good 3 points: I feel some irritation and stickiness while brushing my teeth 2 points: I feel irritation and stickiness while brushing my teeth, and the feeling of use is not good 1 point: I feel strong irritation and stickiness while brushing my teeth, and the feeling of use is poor

Evaluation criteria-2
◎: Average score of 10 people is 4.0 points or more ○: Average score of 10 people is 3.0 points or more and less than 4.0 points △: Average score of 10 people is 2.0 points or more and less than 3.0 points × : Average score of 10 people is less than 2.0

Raw material dextranase used: Sankyo Co., Ltd. Dextranase monolaurate decaglyceryl;
NIKKOL Decaglyn 1-L manufactured by Nippon Chemicals Co., Ltd.
Hexaglyceryl monomyristate;
NIKKOL Hexaglyn 1-M manufactured by Nippon Chemicals Co., Ltd.
Decaglyceryl monomyristate;
NIKKOL Decaglyn 1-M manufactured by Nippon Chemicals Co., Ltd.
Sodium pyrophosphate; manufactured by Taihei Chemical Sangyo Co., Ltd. anhydrous sodium pyrophosphate potassium pyrophosphate: manufactured by Taihei Chemical Sangyo Co., Ltd. potassium pyrophosphate sodium lauryl sulfate: manufactured by Toho Chemical Co., Ltd. sodium lauryl sulfate

Raw material used: Sodium N-lauroylmethyl taurate;
NIKKOL LMT manufactured by Nippon Chemicals Co., Ltd.
Sodium lauroylmethylalanine aqueous solution (30%);
NIKKO Alaninate LN-30 manufactured by Nippon Chemicals Co., Ltd.
Sorbitan monolaurate;
NIKKOL SL-10 manufactured by Nippon Chemicals Co., Ltd.
Polyoxyethylene (20) hydrogenated castor oil;
NIKKOL HCO-20 manufactured by Nippon Chemicals Co., Ltd.
Polyoxyethylene (25) lauryl ether;
NIKKOL BL-25 manufactured by Nippon Chemicals Co., Ltd.
Sodium tripolyphosphate; sodium tripolyphosphate manufactured by Taihei Chemical Industry Co., Ltd.

As can be seen from the results of Tables 1 and 2, when (A) polyglycerin fatty acid ester is not included, and other nonionic surfactant is blended instead, (B) and (C) components are included. However, an excellent plaque removal effect was not obtained. When the blending amount of polyglycerin fatty acid ester is less than 0.5%, there is no irritation or stickiness during tooth brushing and the feeling of use is good, but an excellent plaque removal effect cannot be obtained, and when it exceeds 2% Although the plaque removal effect was improved, the feeling of use was poor (Comparative Examples 1 to 5).
When (B) water-soluble pyrophosphate was not included and sodium tripolyphosphate was blended instead, the effect of removing plaque was inferior even when components (A) and (C) were included. When the amount of water-soluble pyrroline salt is less than 0.5%, the feeling of use is good, but when it exceeds 2%, the effect of removing plaque is high but the feeling of use is poor (comparative example). 6-8).
(C) When other anionic surfactants (N-lauroylmethyltaurine sodium, lauroylmethylalanine sodium) are used in combination in place of the alkyl sulfate, plaque is included even if the components (A) and (B) are included. It was inferior in removal effect, and was also inferior in usability because irritation and stickiness were felt during tooth brushing. When the blending amount of the alkyl sulfate is less than 0.5%, satisfactory use feeling and plaque removal effect cannot be obtained, and when it exceeds 2%, the use feeling is excellent although the plaque removal effect is excellent (Comparative Examples 9 to 12).
When the mass ratio of the component (A) / component (B) is less than 0.5, the plaque removal effect and the feeling of use are inferior, and when it exceeds 4, the satisfactory plaque removal effect and the feeling of use cannot be obtained. (Comparative Examples 13 and 14).
On the other hand, the dentifrice composition containing dextranase was mixed with (A) polyglycerol fatty acid ester in an amount of 0.5 to 2%, (B) sodium pyrophosphate in an amount of 0.5 to 2%, and (C) an alkyl sulfate. Feeling irritation and stickiness while brushing teeth by blending 0.5 to 1.5% of salt and setting the mass ratio of component (A) / component (B) to 0.5 to 4 (Example) In addition, there is no need to increase the amount of enzyme added, so the flavor does not deteriorate, the feeling of use is excellent, and an excellent plaque removal effect is exhibited. Even when sodium fluoride is added, an insoluble salt is produced. It has been found that the object of the present invention can be achieved.

Claims (1)

  To dentifrice composition containing dextranase, (A) polyglycerin fatty acid ester 0.5-2 mass%, (B) water-soluble pyrophosphate 0.5-2 mass%, (C) alkyl A dentifrice composition comprising 0.5 to 1.5% by mass of sulfate and a mass ratio of (A) component / (B) component of 0.5 to 4.