JP2008533500A - 保存生物学的標本のための保管液体としてのフッ素化流体の使用 - Google Patents
保存生物学的標本のための保管液体としてのフッ素化流体の使用 Download PDFInfo
- Publication number
- JP2008533500A JP2008533500A JP2008503086A JP2008503086A JP2008533500A JP 2008533500 A JP2008533500 A JP 2008533500A JP 2008503086 A JP2008503086 A JP 2008503086A JP 2008503086 A JP2008503086 A JP 2008503086A JP 2008533500 A JP2008533500 A JP 2008533500A
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- JP
- Japan
- Prior art keywords
- specimen
- tissue
- formaldehyde
- storage fluid
- storage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
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- 239000012530 fluid Substances 0.000 title claims abstract description 54
- 239000007788 liquid Substances 0.000 title claims description 8
- 229930195733 hydrocarbon Natural products 0.000 claims abstract description 14
- 150000002430 hydrocarbons Chemical class 0.000 claims abstract description 14
- 239000004215 Carbon black (E152) Substances 0.000 claims abstract description 9
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 112
- 238000000034 method Methods 0.000 claims description 25
- 239000000834 fixative Substances 0.000 claims description 18
- 239000000203 mixture Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 238000003809 water extraction Methods 0.000 claims description 6
- 150000001298 alcohols Chemical class 0.000 claims description 3
- 210000001519 tissue Anatomy 0.000 description 74
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 66
- 235000019441 ethanol Nutrition 0.000 description 33
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 28
- 210000003205 muscle Anatomy 0.000 description 15
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 14
- 238000004321 preservation Methods 0.000 description 13
- 230000015556 catabolic process Effects 0.000 description 11
- 230000018044 dehydration Effects 0.000 description 10
- 238000006297 dehydration reaction Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 9
- 241000361919 Metaphire sieboldi Species 0.000 description 9
- 238000006731 degradation reaction Methods 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 230000000052 comparative effect Effects 0.000 description 8
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- DFUYAWQUODQGFF-UHFFFAOYSA-N 1-ethoxy-1,1,2,2,3,3,4,4,4-nonafluorobutane Chemical compound CCOC(F)(F)C(F)(F)C(F)(F)C(F)(F)F DFUYAWQUODQGFF-UHFFFAOYSA-N 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 241001233061 earthworms Species 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 238000013459 approach Methods 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000008098 formaldehyde solution Substances 0.000 description 3
- 208000035404 Autolysis Diseases 0.000 description 2
- 206010057248 Cell death Diseases 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
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- 101150065637 Hfe gene Proteins 0.000 description 2
- 241000243662 Lumbricus terrestris Species 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 230000028043 self proteolysis Effects 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- FRCHKSNAZZFGCA-UHFFFAOYSA-N 1,1-dichloro-1-fluoroethane Chemical compound CC(F)(Cl)Cl FRCHKSNAZZFGCA-UHFFFAOYSA-N 0.000 description 1
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 229920006926 PFC Polymers 0.000 description 1
- 102100038567 Properdin Human genes 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
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- 210000000988 bone and bone Anatomy 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 239000012024 dehydrating agents Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- -1 formalin (eg Chemical compound 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000019432 tissue death Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US66378205P | 2005-03-21 | 2005-03-21 | |
| PCT/US2006/010186 WO2006102304A2 (en) | 2005-03-21 | 2006-03-21 | Use of fluorinated fluids as storage liquid for preserved biological specimens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008533500A true JP2008533500A (ja) | 2008-08-21 |
| JP2008533500A5 JP2008533500A5 (enExample) | 2009-04-23 |
Family
ID=36699059
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008503086A Withdrawn JP2008533500A (ja) | 2005-03-21 | 2006-03-21 | 保存生物学的標本のための保管液体としてのフッ素化流体の使用 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20060223139A1 (enExample) |
| EP (1) | EP1863343A2 (enExample) |
| JP (1) | JP2008533500A (enExample) |
| KR (1) | KR20070112882A (enExample) |
| CN (1) | CN101146446A (enExample) |
| AU (1) | AU2006227220A1 (enExample) |
| WO (1) | WO2006102304A2 (enExample) |
| ZA (1) | ZA200709042B (enExample) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101374596B1 (ko) * | 2009-11-04 | 2014-03-17 | 유니마테크 가부시키가이샤 | 폴리플루오로알킬포스폰산염 유화제 및 이것을 유효성분으로 하는 이형제 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2906650A1 (de) * | 1979-02-21 | 1980-08-28 | Pfrimmer Pharma | Verfahren zur herstellung von transplantatkonserven |
| US4911915A (en) * | 1987-10-13 | 1990-03-27 | Richard-Allan Medical Industries | Method of processing tissue specimens and dehydrant solvent for use therein |
| EP0440925A1 (en) * | 1989-12-13 | 1991-08-14 | The Green Cross Corporation | Internal organ-preserving liquid and method for preserving internal organs |
| US5679333A (en) * | 1996-10-25 | 1997-10-21 | Dunphy; Brian William | Formaldehyde-free tissue preservative compositions |
| US6017725A (en) * | 1997-08-21 | 2000-01-25 | Aaper Alcohol And Chemical Company | Cytological fixative and dehydrating agent for treating histological and cytological tissue |
| US20010055809A1 (en) * | 1998-01-30 | 2001-12-27 | Harpal S. Mangat | Extending tissue preservation |
| CA2335152C (en) * | 1998-05-14 | 2008-11-18 | The Cleveland Clinic Foundation | Processing of implantable animal tissues for dry storage |
| JP4688987B2 (ja) * | 1998-08-31 | 2011-05-25 | 株式会社バイオバンク | 哺乳類動物摘出臓器の保存方法 |
| US6475716B1 (en) * | 2001-03-06 | 2002-11-05 | Biobank Co., Ltd. | Method for preserving mammalian organs |
| US20030049187A1 (en) * | 2001-05-23 | 2003-03-13 | Robert Kaiser | Decontamination system and method of decontamination |
| US20040202993A1 (en) * | 2003-04-10 | 2004-10-14 | Poo Ramon E. | Apparatus and method for organ preservation and transportation |
| US20050160701A1 (en) * | 2003-12-24 | 2005-07-28 | Stevens Timothy A. | Tissue preservation assembly and method of making and using the same |
| GB0426308D0 (en) * | 2004-12-01 | 2004-12-29 | Byotrol Llc | Preservative and embalming fluids |
-
2006
- 2006-03-21 JP JP2008503086A patent/JP2008533500A/ja not_active Withdrawn
- 2006-03-21 KR KR1020077024134A patent/KR20070112882A/ko not_active Withdrawn
- 2006-03-21 AU AU2006227220A patent/AU2006227220A1/en not_active Abandoned
- 2006-03-21 US US11/385,372 patent/US20060223139A1/en not_active Abandoned
- 2006-03-21 CN CNA2006800092721A patent/CN101146446A/zh active Pending
- 2006-03-21 EP EP06748504A patent/EP1863343A2/en not_active Withdrawn
- 2006-03-21 WO PCT/US2006/010186 patent/WO2006102304A2/en not_active Ceased
-
2007
- 2007-10-19 ZA ZA200709042A patent/ZA200709042B/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN101146446A (zh) | 2008-03-19 |
| US20060223139A1 (en) | 2006-10-05 |
| AU2006227220A1 (en) | 2006-09-28 |
| WO2006102304A3 (en) | 2007-05-10 |
| KR20070112882A (ko) | 2007-11-27 |
| WO2006102304A2 (en) | 2006-09-28 |
| EP1863343A2 (en) | 2007-12-12 |
| ZA200709042B (en) | 2008-11-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20090305 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20090305 |
|
| A761 | Written withdrawal of application |
Free format text: JAPANESE INTERMEDIATE CODE: A761 Effective date: 20100618 |