JP2008517278A5 - - Google Patents
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- Publication number
- JP2008517278A5 JP2008517278A5 JP2007536976A JP2007536976A JP2008517278A5 JP 2008517278 A5 JP2008517278 A5 JP 2008517278A5 JP 2007536976 A JP2007536976 A JP 2007536976A JP 2007536976 A JP2007536976 A JP 2007536976A JP 2008517278 A5 JP2008517278 A5 JP 2008517278A5
- Authority
- JP
- Japan
- Prior art keywords
- label
- ubiquitin
- compound
- cks1
- combination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102400001093 PAK-2p27 Human genes 0.000 claims 46
- 238000000034 method Methods 0.000 claims 30
- 102400000757 Ubiquitin Human genes 0.000 claims 25
- 108090000848 Ubiquitin Proteins 0.000 claims 25
- 150000001875 compounds Chemical class 0.000 claims 20
- 101100006503 Drosophila melanogaster Cks30A gene Proteins 0.000 claims 16
- 229920001184 polypeptide Polymers 0.000 claims 9
- 102000004196 processed proteins & peptides Human genes 0.000 claims 9
- 108090000765 processed proteins & peptides Proteins 0.000 claims 9
- 230000034512 ubiquitination Effects 0.000 claims 9
- 238000010798 ubiquitination Methods 0.000 claims 9
- 229910052693 Europium Inorganic materials 0.000 claims 6
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 claims 6
- 239000007850 fluorescent dye Substances 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 6
- 101150073031 cdk2 gene Proteins 0.000 claims 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 4
- 102000003909 Cyclin E Human genes 0.000 claims 4
- 108090000257 Cyclin E Proteins 0.000 claims 4
- 108010004469 allophycocyanin Proteins 0.000 claims 4
- 239000002246 antineoplastic agent Substances 0.000 claims 3
- 238000003556 assay Methods 0.000 claims 3
- 238000002372 labelling Methods 0.000 claims 3
- 230000001093 anti-cancer Effects 0.000 claims 2
- 229960002685 biotin Drugs 0.000 claims 2
- 235000020958 biotin Nutrition 0.000 claims 2
- 239000011616 biotin Substances 0.000 claims 2
- GWQVMPWSEVRGPY-UHFFFAOYSA-N europium cryptate Chemical compound [Eu+3].N=1C2=CC=CC=1CN(CC=1N=C(C=CC=1)C=1N=C(C3)C=CC=1)CC(N=1)=CC(C(=O)NCCN)=CC=1C(N=1)=CC(C(=O)NCCN)=CC=1CN3CC1=CC=CC2=N1 GWQVMPWSEVRGPY-UHFFFAOYSA-N 0.000 claims 2
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 2
- 108010090804 Streptavidin Proteins 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000013537 high throughput screening Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 238000002165 resonance energy transfer Methods 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US61909204P | 2004-10-15 | 2004-10-15 | |
| PCT/US2005/037171 WO2006044747A2 (en) | 2004-10-15 | 2005-10-17 | P27 ubiquitination assay and methods of use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2008517278A JP2008517278A (ja) | 2008-05-22 |
| JP2008517278A5 true JP2008517278A5 (enExample) | 2008-12-04 |
Family
ID=36203595
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2007536976A Pending JP2008517278A (ja) | 2004-10-15 | 2005-10-17 | ｐ２７のユビキチン化アッセイ及びその使用方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20060194262A1 (enExample) |
| EP (1) | EP1810008A4 (enExample) |
| JP (1) | JP2008517278A (enExample) |
| AU (1) | AU2005295517A1 (enExample) |
| CA (1) | CA2584131A1 (enExample) |
| WO (1) | WO2006044747A2 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010510771A (ja) * | 2006-11-27 | 2010-04-08 | シ ビオ アンテルナショナル | 翻訳後修飾の検出のための方法 |
| MX344557B (es) * | 2007-05-08 | 2016-12-20 | Hunter Douglas Ind Switzerland | Sistema de color multivariado con aplicacion de textura. |
| JP2011528908A (ja) * | 2008-07-25 | 2011-12-01 | プロジェンラ インク． | ユビキチンリガーゼのモジュレーターの特定法 |
| GB201007185D0 (en) * | 2010-04-29 | 2010-06-09 | Iti Scotland Ltd | Ubiquitination assay |
| EP2694964B1 (en) * | 2011-04-07 | 2019-06-26 | The Scripps Research Institute | High-throughput screening for compounds modulating levels of cellular macromolecules |
| CN103196877B (zh) * | 2013-03-08 | 2015-06-03 | 刘小龙 | 基于均相时间分辨荧光的检测重金属含量的方法 |
| CN104155452A (zh) * | 2013-05-15 | 2014-11-19 | 复旦大学 | 一种检测细胞内蛋白质降解的方法 |
| GB201415688D0 (en) * | 2014-09-04 | 2014-10-22 | Univ Edinburgh | Method and Systems |
| WO2018029682A1 (en) * | 2016-08-08 | 2018-02-15 | Ramot At Tel-Aviv University Ltd. | Bacterial systems for analyzing ubiquitylated polypeptides |
| EP3665283A4 (en) | 2017-08-08 | 2021-04-21 | Technology Innovation Momentum Fund (Israel) Limited Partnership | CHLORAMPHENICOL RESISTANT TRUNCATED PROTEIN AND ITS USES |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2174140C (en) * | 1993-10-28 | 2004-04-06 | Kenneth L. Beattie | Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions |
| US5463564A (en) * | 1994-09-16 | 1995-10-31 | 3-Dimensional Pharmaceuticals, Inc. | System and method of automatically generating chemical compounds with desired properties |
| US6747128B2 (en) * | 1997-08-20 | 2004-06-08 | Gpc Biotech, Inc. | Components of ubiquitin ligase complexes, and uses related thereto |
| US6413725B1 (en) * | 1998-08-07 | 2002-07-02 | California Institute Of Technology | Biochemical assay to monitor the ubiquitin ligase activities of cullins |
| NZ510312A (en) * | 1998-08-28 | 2004-01-30 | Univ New York | A nucleic acid encoding an F-box protein |
| US6740495B1 (en) * | 2000-04-03 | 2004-05-25 | Rigel Pharmaceuticals, Inc. | Ubiquitin ligase assay |
| JP4121854B2 (ja) * | 2001-01-05 | 2008-07-23 | ニューヨーク・ユニバーシティ | 増殖性および分化性疾患の治療に有用な化合物の同定方法 |
| DE60220621T2 (de) * | 2001-11-09 | 2008-03-06 | Proteologics, Inc. | Posh Nukleinsäure, Polypeptide und darauf bezogene Verfahren |
| WO2003100064A1 (en) * | 2002-05-29 | 2003-12-04 | Kyowa Hakko Kogyo Co., Ltd. | Novel ubiquitin ligase |
-
2005
- 2005-10-17 AU AU2005295517A patent/AU2005295517A1/en not_active Abandoned
- 2005-10-17 EP EP05815021A patent/EP1810008A4/en not_active Withdrawn
- 2005-10-17 JP JP2007536976A patent/JP2008517278A/ja active Pending
- 2005-10-17 WO PCT/US2005/037171 patent/WO2006044747A2/en not_active Ceased
- 2005-10-17 US US11/253,128 patent/US20060194262A1/en not_active Abandoned
- 2005-10-17 CA CA002584131A patent/CA2584131A1/en not_active Abandoned
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