JP2008253263A5 - - Google Patents
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- Publication number
- JP2008253263A5 JP2008253263A5 JP2008094907A JP2008094907A JP2008253263A5 JP 2008253263 A5 JP2008253263 A5 JP 2008253263A5 JP 2008094907 A JP2008094907 A JP 2008094907A JP 2008094907 A JP2008094907 A JP 2008094907A JP 2008253263 A5 JP2008253263 A5 JP 2008253263A5
- Authority
- JP
- Japan
- Prior art keywords
- sample
- temperature
- target rna
- cdna
- pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002299 complementary DNA Substances 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 238000003757 reverse transcription PCR Methods 0.000 claims 2
- 102100034343 Integrase Human genes 0.000 claims 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 230000001419 dependent effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000011541 reaction mixture Substances 0.000 claims 1
- 238000005382 thermal cycling Methods 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP07007173 | 2007-04-05 | ||
| EP07007173.3 | 2007-04-05 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2008253263A JP2008253263A (ja) | 2008-10-23 |
| JP2008253263A5 true JP2008253263A5 (OSRAM) | 2010-04-08 |
| JP5191041B2 JP5191041B2 (ja) | 2013-04-24 |
Family
ID=38453943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2008094907A Active JP5191041B2 (ja) | 2007-04-05 | 2008-04-01 | 急速ワンステップｒｔ−ｐｃｒ |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US8119353B2 (OSRAM) |
| EP (1) | EP1978109B1 (OSRAM) |
| JP (1) | JP5191041B2 (OSRAM) |
| ES (1) | ES2398984T3 (OSRAM) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9175332B2 (en) * | 2010-07-29 | 2015-11-03 | Roche Molecular Systems, Inc. | Generic PCR |
| CN103154270B (zh) | 2010-10-04 | 2018-01-09 | 霍夫曼-拉罗奇有限公司 | 用于rt‑pcr反应缓冲液中的细胞裂解的方法 |
| WO2013101290A1 (en) * | 2011-12-29 | 2013-07-04 | Qiagen | Methods of using telomeres as markers for aging |
| PT3539666T (pt) | 2012-05-24 | 2021-09-23 | Univ Utah Res Found | Pcr extrema |
| DK3337615T3 (da) * | 2015-11-05 | 2022-05-09 | Univ Utah Res Found | Ekstrem pcr via revers transkription |
| JP2018023360A (ja) * | 2016-07-29 | 2018-02-15 | セイコーエプソン株式会社 | 核酸増幅反応方法、試薬、および試薬の使用方法 |
| GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
| MX2021008567A (es) * | 2019-01-17 | 2021-09-21 | Univ Northwestern | Reaccion en cadena de la polimerasa cuantitativa con retrotranscripcion rapida. |
| JP7180944B1 (ja) | 2020-12-25 | 2022-11-30 | 株式会社ニッポンジーン | 改変型ｄｎａポリメラーゼ |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5105599A (en) * | 1989-02-24 | 1992-04-21 | Highland Supply Corporation | Means for securing a decorative cover about a flower pot |
| US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US5561058A (en) | 1986-08-22 | 1996-10-01 | Hoffmann-La Roche Inc. | Methods for coupled high temperatures reverse transcription and polymerase chain reactions |
| US5118801A (en) * | 1988-09-30 | 1992-06-02 | The Public Health Research Institute | Nucleic acid process containing improved molecular switch |
| EP0506889B1 (en) | 1989-12-22 | 1997-04-02 | F. Hoffmann-La Roche AG | High temperature reverse transcriptases |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| JPH07501712A (ja) | 1992-09-29 | 1995-02-23 | シーダーズ−サイナイ・メディカル・センター | 微量ｒｎａまはたｒｎａおよびｄｎａ検出用の一段ｒｎａおよび結合一段ｒｎａおよびｄｎａポリメラーゼ・チェイン・リアクション |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5876924A (en) * | 1994-06-22 | 1999-03-02 | Mount Sinai School Of Medicine | Nucleic acid amplification method hybridization signal amplification method (HSAM) |
| DK1179600T3 (da) * | 1996-06-04 | 2005-09-05 | Univ Utah Res Found | Overvågning af hybridisering under PCR |
| NZ333135A (en) | 1996-06-04 | 2000-02-28 | Univ Utah Res Found | Systems and methods for monitoring for DNA amplification by fluorescence using acceptor fluorophore labeled nucleic acid probe and a donor fluorophore labeled nucleic acid probe which hybridise to adjacent regions of a target sequence |
| US7179590B2 (en) * | 2000-04-18 | 2007-02-20 | Roche Molecular Systems, Inc | High temperature reverse transcription using mutant DNA polymerases |
| JP4808365B2 (ja) * | 2001-03-02 | 2011-11-02 | ユニバーシティ オブ ピッツバーグ オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション | Ｐｃｒ法 |
| US20030108898A1 (en) * | 2001-07-03 | 2003-06-12 | Paul Choppa | Method for detection, quantitation and breakpoint cluster region determination of fusion transcripts |
| ATE533857T1 (de) * | 2003-01-17 | 2011-12-15 | Univ Hong Kong Chinese | Zirkulierende mrna als diagnostische marker für erkankungen die mit einer schwangerschaft zusammenhängen |
-
2008
- 2008-04-01 JP JP2008094907A patent/JP5191041B2/ja active Active
- 2008-04-03 EP EP08006778A patent/EP1978109B1/en active Active
- 2008-04-03 ES ES08006778T patent/ES2398984T3/es active Active
- 2008-04-03 US US12/061,700 patent/US8119353B2/en active Active
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