JP2008074757A - Skin care preparation for preventing aging - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation for preventing aging, which exhibits high aging-preventing effects, especially wrinkle-ameliorating effects by using a small amount of the preparation. <P>SOLUTION: The skin care preparation for preventing the aging contains one or more selected from an oligopeptide and an acylated derivative thereof, and an extract of a plant of the genus Duabanga. An oligopeptide originated from a seed of okra, an oligopeptide originated from a seed of corn, or an oligopeptide obtained by hydrolyzing a collagen contained in the skin of fish is used as the oligopeptide. A palmitoyl oligopeptide is used as the acyl derivative of the oligopeptide. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to an anti-aging skin external preparation that exhibits an excellent wrinkle improving effect.

  Conventionally, wrinkles have been cited as the most worrisome phenomenon of skin aging symptoms. There are several types of wrinkles, such as facial wrinkles caused by repeated facial muscle contractions in the same place, creased wrinkles caused by dry skin, and aging wrinkles caused by aging of the dermis structure. Are known.

  Conventionally, there has been a means of using a botulinum toxin as a method for resolving facial expression wrinkles that have been made (see Patent Document 1). That is, botulinum toxin is injected subcutaneously into, for example, the eyebrows or the corners of the eyes, and the skin where the wrinkles are formed is stretched by the muscle-relaxing action possessed by the toxin, thereby eliminating facial wrinkles.

  However, botulinum toxin has been used for the treatment of various dystrophies for a long time, and has a fear of causing undesirable side effects. Moreover, since it is limited to administration as an injection, it has not been used by individuals. Therefore, there is a need for a component having a muscle relaxing action that can be blended in an external preparation for skin, which can be easily used by individuals without side effects, and various plant extracts having a muscle relaxing action (see Patent Document 2), Proteins derived from okra seeds (see Patent Document 3) and the like are disclosed.

  In addition, for the purpose of preventing and improving aging wrinkles, products for external application and internal use of collagen, which is a major component of the dermis, and external preparations for skin containing substances that promote collagen production are on the market. Acylated oligopeptides are known as components that promote collagen production in the dermis and are effective in preventing and improving wrinkles and talmi (see Patent Documents 4 and 5).

  In addition, it is already known that an external preparation for skin containing an extract of a plant belonging to the genus Duabanga has a moisturizing effect and an effect of improving skin roughness (Patent Document 6).

  However, it has not been known so far to combine one or two or more selected from oligopeptides and acylated derivatives thereof and a Duabanga plant extract into a topical skin preparation.

Special table 2003-505343 gazette JP 2005-206466 A JP-T-2001-518910 JP 2004-043366 A JP 2006-176431 A JP 2003-335620 A

  In this invention, it was made into the subject to provide the skin anti-aging agent for anti-aging which exhibits a high anti-aging effect, especially a wrinkle improvement effect with a small quantity of compounding.

  As a result of intensive studies to achieve the above object, by combining one or more selected from oligopeptides and acylated derivatives thereof, and Duabanga plant extract in combination with a topical skin preparation, The inventors have found that the effect of preventing aging, particularly the effect of improving wrinkles, is synergistically improved, and the present invention has been completed.

  That is, this invention provides the skin external preparation for anti-aging containing 1 type, or 2 or more types selected from an oligopeptide and its acylated derivative | guide_body, and Duabanga genus plant extract.

  The present invention also relates to one or two kinds of oligopeptides derived from okra seeds, oligopeptides derived from corn seeds, oligopeptides obtained by hydrolyzing collagen contained in fish skin, and palmitoyl oligopeptides. Provided is an anti-aging skin external preparation containing an oligopeptide and an acylated derivative thereof and a Duavanga plant extract.

  According to the present invention, it exhibits an excellent wrinkle improving effect and is particularly useful as an anti-aging skin external preparation.

  An embodiment of the present invention will be described.

  The skin anti-aging agent for anti-aging of the present invention contains one or more selected from oligopeptides and acylated derivatives thereof, and Duavanga plant extracts.

  The oligopeptide and acylated derivative thereof used in the present invention will be described in detail.

  As the oligopeptide used in the present invention, an oligopeptide having 2 to several tens of amino acids, preferably 2 to 10 amino acids, and a salt thereof are used. The kind of amino acid which comprises an oligopeptide is not specifically limited. Such oligopeptides can be synthesized from amino acids, but oligopeptides obtained by hydrolyzing biological proteins may also be used. The origin of such oligopeptide is not particularly limited, and examples thereof include okra seed, corn seed, carrot, apple seed, almond seed, soybean, pea, fish, silk and the like. As the plant-derived oligopeptide used in the present invention, it is preferable to use one or two kinds selected from okra seed-derived oligopeptides and corn seed-derived oligopeptides for the purpose of improving expression wrinkles. In addition, as the oligopeptide derived from fish, it is preferable to use collagen or elastin hydrolyzate contained in fish skin, scales, fins, floats and the like. Among these fish, it is preferable to use an oligopeptide obtained by hydrolyzing collagen contained in fish skin from the viewpoint of stable supply of raw materials and easy molecular weight reduction. As the fish, salmon is particularly preferable.

  An acylated derivative of the oligopeptide used in the present invention is not particularly limited, but 2 to 6 amino acids, which are selected from lysine, threonine, serine, glycine, histidine, arginine, and phenylalanine, are bound. It is preferred to use acylated derivatives of oligopeptides. As the acyl group bonded to the oligopeptide, a branched or straight chain acyl group having 8 to 22 carbon atoms is preferably used, and palmitoyl is used from the viewpoint of its affinity for skin and safety such as skin irritation. It is particularly preferred to use a group. As an acylated derivative of such oligopeptide, commercially available SYN-COLL (made by Pentafarm), MATRIXYL (made by SEDERMA) and the like can also be used.

  The blending amount of these oligopeptides and acylated derivatives thereof in the external preparation for skin is not particularly limited depending on the intended effect, but is preferably 0.0001 to 5% by mass, more preferably based on the total amount of external preparation for skin. 0.0001-3 mass%, More preferably, 0.005-3 mass% can be mix | blended. Even when blended in an amount of 5% by mass or more, the improvement of the effect of the present invention is not recognized.

  Subsequently, the Duabanga plant used in the present invention will be described.

The plant of the genus Duavanga used in the present invention is a high tree belonging to the family Sonneratiaceae , and is an evergreen or deciduous high tree distributed from eastern Himalaya to New Guinea, and three types have been confirmed. Duabanga Grandi Flora (Duabanga grandiflora Walp .; Duabanga sonneratioides Buch. -Ham.) , The stem by Takagi deciduous stands vertically, the height 10 to 40 m. The material is used for building materials and tea boxes, and the fruit is sour and sometimes edible. Widely distributed from Borneo to New Guinea, Duabanga moluccana Bl. Is a large Takagi with a height of 50-60m, and grows fast. Yes.

  Next, a method for obtaining an extract from Duabanga plants will be described. The above-mentioned plant has one or more parts such as leaves, stems, stems, branches, branches and leaves, fruit skin, fruits, bark, sap, seeds, rhizomes, root barks, roots, flower heads, flower heads, flowers, etc. Or use it after drying. As a method for obtaining a component from a plant of the genus Duabanga, the plant body may be used as it is, or a processed product such as squeezed may be obtained, or extraction may be performed using an extraction solvent. The extraction solvent is not particularly limited, and monohydric alcohols such as water, ethanol, methanol, isopropanol, isobutanol, n-hexanol, methylamyl alcohol, 2-ethylbutanol, n-octyl alcohol, glycerin, ethylene glycol, ethylene Glycolic monomethyl ether, propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triethylene glycol, 1,3-butylene glycol, hexylene glycol and other polyhydric alcohols or derivatives thereof, acetone, methyl ethyl ketone, methyl isobutyl ketone, methyl ketones such as -n-propyl ketone, esters such as ethyl acetate and isopropyl acetate, ethers such as ethyl ether, isopropyl ether and n-butyl ether , Hydrocarbons such as squalane, petrolatum, paraffin wax, paraffin oil, olive oil, wheat germ oil, rice oil, sesame oil, macadamian nut oil, almond oil, coconut oil and other vegetable oils, beef fat, pork fat, whale oil, etc. Animal fats and oils are exemplified. In addition, polar solvents to which inorganic salts such as phosphate buffered saline are added and solvents to which surfactants are added can also be used, and further, water, carbon dioxide, ethylene, propylene, ethane, propane, dinitrogen monoxide. One or more supercritical fluids such as chlorodifluoromethane, chlorotrifluoromethane, xenon, ammonia, methanol, ethanol, etc., or a subcritical fluid may be used without any particular limitation.

  The ratio of the plant and the solvent in the extraction is not particularly limited, but is preferably 0.1 to 1000 times by weight of the solvent with respect to the plant 1, and more preferably 0.5 to 100 times by weight in terms of extraction operation and efficiency. The extraction temperature is conveniently in the range from 0 ° C. to the boiling point of the solvent, and the extraction time is preferably in the range of 1 hour to 2 weeks, although it varies depending on the extraction temperature.

  As for the extract obtained from the plant of the genus Duavanga thus obtained, each extract can be used as it is, but as long as the effect is not lost, purification operations such as deodorization, decolorization, concentration, etc. are added, and further May be used as a fraction using column chromatography or the like. These extracts, purified products, and fractions can be dried by removing the solvent from them, and can also be used in a form solubilized in a solvent such as alcohol, or in the form of an emulsion. it can.

  In the anti-aging skin external preparation of the present invention, one or two or more selected from oligopeptides and acylated derivatives thereof, and Duabanga plant extract are used as an oligopeptide and 1 part by weight of Duabanga plant extract. It is preferable to blend 0.01 to 10 parts by mass of one or more selected from the acylated derivatives.

  In the anti-aging skin external preparation of the present invention, by using one or more selected from oligopeptides and acylated derivatives thereof in combination with Duabanga plant extracts, anti-aging such as wrinkle improvement is achieved. The effect is synergistically improved.

  The anti-aging skin external preparation of the present invention can be provided in various dosage forms such as lotions, emulsions, gels, creams, ointments, powders, granules and the like. In addition, skin cosmetics such as lotion, milky lotion, cream, beauty essence, packs, foundation cosmetics such as makeup base lotion and makeup base cream, foundations for each dosage form such as emulsion, oily, solid, etc. It can be provided as makeup cosmetics such as color and teak color, and body cosmetics such as hand cream, leg cream, neck cream and body lotion.

  The anti-aging skin external preparation according to the present invention includes, in addition to the above-mentioned vitamins, common pharmaceuticals such as oil components, surfactants, pigments, ultraviolet absorbers, fragrances, antibacterial and antifungal agents, and cosmetics. A raw material can be included.

  Furthermore, 1 type, or 2 or more types selected from an active oxygen scavenger, a moisturizer, a cell activator, and an anti-inflammatory agent can be mix | blended with the skin external preparation for anti-aging of this invention.

  Although the formulation example and use test of the anti-aging skin external preparation of this invention are demonstrated in detail below, the technical scope of this invention is not limited at all by this.

<Production Example 1>
The dried Duabanga grandiflora leaves were immersed in a 20% by volume 50% by volume ethanol aqueous solution for 7 days at room temperature. The extract was filtered, concentrated and dried under reduced pressure to obtain an extract.

<Production Example 2>
The dried Duavanga Grandiflora leaves were extracted by stirring at 120 ° C. for 2 hours in 20 parts by weight of purified water. The extract was filtered to obtain the desired extract.

  The cosmetics which concern on Examples 1-4 which concern on the skin external preparation for anti-aging of this invention, and Comparative Examples 1-5 by the prescription shown in Table 1 and Table 2 were prepared. The cosmetic liquid was prepared by adding the phase A dissolved at 70 ° C. to the phase B heated to 70 ° C., emulsifying with a homomixer, cooling to 40 ° C., and adding the phase C. . In the examples, as palmitoyl oligopeptide 1, SYN-COLL (made by Pentafarm) was used, and as palmitoyl oligopeptide 2, MATRIXYL (made by SEDERMA) was used. The same applies to the embodiments described below.

  Using Examples 1 to 4 and Comparative Examples 1 to 5 described above, an actual use test for two months was performed. As panelists, women in their 40s to 60s who had skin symptoms such as significant wrinkles or reduced elasticity were used, and each group consisted of 20 people. The use test was conducted by causing each group to use each of the examples and comparative examples blindly, and observing the state of the skin before the start of the use test and after the end of the use test. Each improvement situation of wrinkles and skin elasticity was evaluated in three stages of “improvement; 2 points”, “slight improvement; 1 point”, “no change; 0 points”, the average value was calculated, and the results are shown in Table 1. The results are shown in Table 2.

  As shown in Tables 1 and 2, the examples of the present invention showed much better wrinkle and skin elasticity improvement effects than the comparative examples in which the active ingredient was blended in an amount of 3 times alone.

[Example 2] Skin cream
(1) Beeswah 6.00 (mass%)
(2) Cetanol 5.00
(3) Reduced lanolin 8.00
(4) Squalane 27.50
(5) Fatty acid glycerin 4.00
(6) Lipophilic glyceryl monostearate 2.00
(7) Polyoxyethylene (20EO)
Sorbitan monolaurate 2.00
(8) Propylene glycol 5.00
(9) Methyl parahydroxybenzoate 0.10
(10) Purified water 38.10
(11) Ethanol 2.00
(12) Corn-derived hydrolyzed protein 0.05
(13) Production Example 2 0.05
(14) Fragrance 0.20
Production method: The oil phase components (1) to (7) are mixed, dissolved and made uniform, and heated to 75 ° C. On the other hand, the water phase components (8) to (10) are mixed and dissolved and heated to 75 ° C. Subsequently, after adding an oil phase component to the said water phase component and pre-emulsifying, it emulsifies uniformly with a homomixer. Thereafter, the mixture is cooled, and (11) to (14) are sequentially added and mixed at 50 ° C.

[Example 3] Water-in-oil emollient cream
(1) Liquid paraffin 30.00 (mass%)
(2) Microcrystalline wax 2.00
(3) Vaseline 5.00
(4) Diglyceryl dioleate 5.00
(5) Sodium L-glutamate 1.60
(6) L-serine 0.40
(7) Propylene glycol 3.00
(8) Methyl paraoxybenzoate 0.10
(9) Purified water 47.70
(10) Palmitoyl oligopeptide 2 0.05
(11) Production Example 1 0.05
(12) Ethanol 5.00
(13) Fragrance 0.10
Production method: Dissolve (5) and (6) in a part of (9) to 50 ° C., and gradually add to (4) heated to 50 ° C. while stirring. This is mixed in advance and uniformly dispersed in (1) to (3) heated and dissolved at 70 ° C. (7) and (8) are added to the remainder of (10), and the mixture heated to 70 ° C. is added with stirring and emulsified with a homomixer. After cooling, add and mix (11) to (13) at 40 ° C.

[Example 4] Hand cream
(1) Cetanol 4.00 (mass%)
(2) Vaseline 2.00
(3) Liquid paraffin 10.00
(4) Glyceryl monostearate ester 1.50
(5) Polyoxyethylene (60EO) glyceryl 2.50
Isostearic acid ester
(6) Tocopherol acetate 0.25
(7) Glycerin 20.00
(8) Methyl paraoxybenzoate 0.10
(9) Purified water 54.45
(10) Palmitoyl oligopeptide 1 0.05
(11) Production Example 2 0.05
(12) Ethanol 5.00
(13) Fragrance 0.10
Production method: The oil phase components (1) to (6) are mixed and dissolved to 75 ° C. On the other hand, the aqueous phase components (7) to (9) are mixed and dissolved to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer. After cooling, the mixture (10) to (13) mixed and homogenized at 40 ° C. is added.

[Example 5] Pack
(1) Purified water 69.30 (mass%)
(2) Polyvinyl alcohol 12.50
(3) Ethanol 10.00
(4) Glycerin 5.00
(5) Polyethylene glycol (average molecular weight 1540) 3.00
(6) Okra seed-derived hydrolyzed protein 0.05
(7) Production Example 1 0.05
(8) Fragrance 0.10
Production method: Components (2) to (8) are sequentially added to (1) to mix, dissolve and homogenize.

[Example 6] Cosmetic liquid
(1) Hydrogenated palm oil 3.000 (mass%)
(2) Behenyl alcohol 4,000
(3) Stearic acid 2.000
(4) Self-emulsifying glyceryl monostearate 3.000
(5) Cetyl palmitate 0.500
(6) Macadamia nut oil fatty acid phytosteryl 1.500
(7) Squalane 4.500
(8) Glyceryl tri-2-ethylhexanoate 6.00
(9) Dimethylpolysiloxane 1.000
(10) Retinol palmitate 0.250
(11) d-δ-tocopherol 0.050
(12) Sodium hyaluronate 0.010
(13) Palmitoyl oligopeptide 1 0.500
(14) Purified water
(15) Hydroxyethyl cellulose (1% by weight aqueous solution) 15.000
(16) 1,3-butylene glycol 9.000
(17) Glycerin 5.000
(18) Methyl paraoxybenzoate 0.100
(19) Daylily extract 0.070
(20) Okra seed-derived oligopeptide 0.150
(21) Almond extract 0.050
(22) Crust derived oligopeptide 0.010
(23) Palmitoyl oligopeptide 2 0.100
(24) Corn seed-derived oligopeptide 0.010
(25) Birch extract 0.010
(26) Time extract 0.010
(27) Altea extract 0.010
(28) Production Example 1 0.100
Production method: The oil phase components (1) to (15) and the water phase components (16) to (20) are heated and dissolved at 75 ° C., respectively. The aqueous phase is added to the oil phase to emulsify and cooled to 40 ° C. Add (21) to (30) and mix.

[Use test]
Twenty women in their 30s to 50s who worried about wrinkles were allowed to use the serum shown in Example 6 twice a day for 3 months. Replicas of skin before and after use were collected and used to measure wrinkle depth. The wrinkle depth irradiates a replica that is aligned in a certain direction from a certain direction obliquely upward with a fiberscope. A shadow pattern caused by light is imaged with a CCD camera. The wrinkle depth was calculated with the area of the shadow pattern as the wrinkle depth. The results are the average values of 20 subjects and are shown in FIG.

  As shown in Table 3 and FIG. 1, the continuous use of the cosmetic liquid of the present invention reduced the wrinkle depth by an average of 44%.

It is a figure which shows the depth of the wrinkle before and behind a use test.

Claims (2)

A skin external preparation for anti-aging containing one or more selected from oligopeptides and acylated derivatives thereof, and a plant extract of Duabanga. One or two oligopeptides selected from okra seed-derived oligopeptides, corn seed-derived oligopeptides, oligopeptides obtained by hydrolyzing collagen contained in fish skin, and palmitoyl oligopeptides and acyls thereof The skin external preparation for anti-aging of Claim 1 containing an aging derivative.

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