JP2008063332A - Immature persimmon fruit powder composition and method for producing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a lipid metabolism improving agent, an excretion enhancing agent of bile acids, an antiobestic agent and an evacuation-enhancing agent that are simply and inexpensively produced. <P>SOLUTION: An immature persimmon fruit powder composition, dried, subsequently crushed and containing 5.5-10% moisture and 3.3-15% tannic acid is provided. The lipid metabolism improving agent, the excretion enhancing agent of bile acids, the antiobestic agent, the evacuation enhancing agent and health supplement foods containing the immature persimmon fruit powder composition are provided. The immature persimmon fruit powder composition can be produced by a production method comprising a process of removing the calyxes from immature persimmon fruits, a cutting process of cutting the immature persimmon fruits into small pieces in a state including pericarp, a drying process of drying the immature persimmon fruits cut into small pieces to remove moisture and a pulverizing process of crushing and pulverizing the dried pieces of immature persimmon fruits. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a method for producing a dried and pulverized immature persimmon fruit powder composition from immature persimmon fruit picked between flowering and two weeks before maturity, and a lipid containing the immature persimmon fruit powder composition The present invention relates to a metabolism improving agent, a bile acid excretion promoter, and further an anti-obesity agent and a defecation promoter.

  In recent years, with the increase in late-night or 24-hour restaurants and convenience stores, the lifestyle of modern people has changed greatly. In particular, the impact on the food environment is significant, and an increasing number of people are unable to eat three meals a day in the morning, noon and evening. In addition, the preference for food has also changed greatly, and by continuing to eat foods containing a large amount of lipids such as hamburgers and pizzas from early childhood, the tendency to prefer these Western-style meals containing lipids has increased, Japanese cuisine, mainly fish and vegetables from ancient Japan, is often shunned by young people.

  Therefore, excessive intake of a diet rich in lipids and an irregular life such as lack of sleep or exercise may increase the concentration of lipids (eg, neutral fat and cholesterol) in the blood. The number of people diagnosed with “hyperlipidemia”, a type of lifestyle-related disease, will increase. This hyperlipidemia is considered to be the main cause of arteriosclerosis, and it is highly likely to endanger life such as myocardial infarction and stroke, and the risk of developing a more serious disease is extremely high. Is. However, since patients suffering from hyperlipidemia have almost no subjective symptoms, they do not receive early diagnosis at a medical institution and cannot judge their body condition objectively. , Tend to worsen symptoms more. In addition, even if a patient is diagnosed with hyperlipidemia through regular physical examinations at the workplace, etc., since there is no subjective symptom due to the lack of subjective symptoms, there is almost no response to such symptoms. Many people do not.

  Therefore, various researches and developments are underway for the purpose of reducing the concentration of lipids in the blood and preventing the progression of arteriosclerosis. Usually, the best way to prevent arteriosclerosis is to avoid eating a high lipid diet. Therefore, the diet that controls the content and amount of meals by calorie calculation etc. is the simplest in order to consume the minimum amount of lipids in daily life and prevent extra lipids from entering the body as much as possible. Has been done. However, the success of this diet requires appropriate advice from the doctor and the patient's own will, and in the case of hyperlipidemic patients with few subjective symptoms, such a will last for a long time. It was very difficult to keep it.

  Therefore, means other than diet therapy are being studied. For example, for the purpose of reducing the blood level of the above-mentioned lipids (particularly cholesterol), it has been found that the blood cholesterol level is significantly reduced by ingesting sputum-derived condensed polyphenol (Patent Document 1). reference). Condensed polyphenol compounds having a certain function as cholesterol metabolism-improved products are almost all contained in general fruits (fruits), although there are some differences in content depending on the fruit type. In addition, there is an example in which a patent application has been found by ingesting dietary fiber derived from cassis fruit by finding an effect of suppressing blood cholesterol elevation (Patent Document 2). Furthermore, there is an example in which a patent application has been filed for the cholesterol-reducing effect of a hydrophilic component derived from citrus fruits or eriocitrin (Patent Document 3). In addition, those using components such as polyphenol obtained by extraction from fruits are also seen (Patent Documents 4 and 5).

  However, the conventional method for obtaining an active ingredient from fruits has a problem that a complicated processing step such as extraction with a solvent such as alcohol and further purification is required, resulting in an increase in production cost. For example, the extraction of the above-mentioned condensed polyphenol compound (bamboo tannin) is performed by crushing (mashing) the koji fruit (mature fruit) as a raw material, adding an aqueous ethanol solution to the milled product, and dipping it for several days. The filtrate obtained by placing at room temperature and filtering the supernatant was passed through a column filled with an adsorbent, then the column was washed, and the adsorbed components were eluted again using an aqueous ethanol solution. The eluate obtained was concentrated under reduced pressure and then purified by lyophilization. That is, for extraction of such tannins, 1) grinding, 2) addition of alcohol, 3) incubation at room temperature, 4) filtration, 5) adsorption to the column, 6) elution from the column, 7) concentration under reduced pressure, 8) It was necessary to go through complicated processing steps such as freeze-drying. Moreover, the condensation type polyphenol compound obtained by the said process was able to obtain only the yield of about 500g with respect to 10 kg (raw material) of a persimmon fruit. That is, the treatment for extracting the condensed polyphenol compound using an aqueous ethanol solution has a low yield of the product obtained with respect to the raw material, and increases the production cost for the complicated and troublesome treatment.

  In addition, obesity and constipation caused by lifestyle habits and dietary habits are neglected compared to other illnesses, despite being troubled by many people. For obesity, lifestyle improvement guidance and diet therapy are performed, and in addition to lifestyle improvement guidance, drugs are used to improve constipation. Recently, many health foods and foods for specified health use for the purpose of anti-obesity and promotion of defecation have appeared.

On the other hand, in general persimmon cultivation, if a large number of persimmons are grown on a single tree, the fruit becomes small and the product value decreases. At each stage of the process, “plucking” and “plucking” operations are performed. A large amount of immature fruit removed by this fruit picking operation or the like was disposed of without any other use. Even for mature fruits, a large amount of non-standard fruits were also disposed of at the selection ground because they were stiff or not shaped. Such non-standard fruits and the above-extracted fruits are also carefully cultivated fruits, and instead of discarding them as they are, it has been desired to divert them to some use and effectively use them.
JP 2003-231684 A JP 2006-76954 A JP 2005-225847 A JP 11-318347 A Japanese Patent Laid-Open No. 10-330278

  In view of the above-mentioned facts, the present invention provides a method for producing an immature strawberry fruit powder composition obtained by drying and pulverizing an immature strawberry fruit powder that has been picked between flowering and 2 weeks before maturation, and the immature strawberry fruit powder composition. The present invention provides a lipid metabolism improving agent or a bile acid excretion promoter.

  Furthermore, in order to eliminate the problems of obesity and constipation that many people have, anti-obesity that expresses a certain defecation promotion effect by containing the immature persimmon fruit powder composition and is suitable for long-term use It provides a defecation promoter.

  As a result of intensive research to solve the above problems, the lipid metabolism improvement action, the bile acid excretion promotion action, and the anti-obesity effect that are excellent in immature fruits picked between flowering and 2 weeks before maturation It was found to have an action and a defecation promoting action. An immature fruit powder composition obtained from such immature fruit before harvest as a raw material, a lipid metabolism improving agent containing the immature persimmon fruit powder composition, a bile acid excretion promoter, an anti-obesity agent, and defecation Succeeded in producing accelerators.

  The immature persimmon fruit used as a raw material for the immature persimmon fruit powder composition of the present invention is an immature persimmon fruit that has been harvested between flowering and harvesting, an immature persimmon fruit that has been harvested for production adjustment before harvesting, etc. This refers to immature persimmon fruits that have little experience in food.

  In addition, although there is no clear standard about discrimination | determination of the matured cocoon and immature cocoon in a strawberry fruit, in this invention, what was picked 2 weeks or more before harvesting from flowering is made into an immature fruit.

  The immature persimmon fruits harvested more than two weeks before the harvesting from the above-mentioned flowering are the young fruits obtained by the “plucking” operation performed at each stage in the growth process from the persimmon flowering to the harvesting of the mature fruit, or It contains immature strawberries that have been harvested to adjust the yield before harvesting. Examples of the types of immature persimmon fruits include, for example, Fuyu persimmon, bee persimmon, flat seedless persimmon, and Tone Hayao persimmon, etc., but the raw material for the immature persimmon fruit powder composition of the present invention includes these It is not limited to the type of salmon.

  The immature persimmon fruit powder composition of the present invention, using immature persimmon fruit as a raw material, is dried and removed until the moisture content in the immature persimmon fruit falls below a predetermined moisture content, dried, For example, an immature persimmon fruit powder composition can be obtained by pulverization by a pulverizing means such as a ball mill.

  The immature persimmon fruit powder composition of the present invention can contain the fruit and skin as it is, in other words, the whole fruit as compared with the conventional method of extracting a specific component as an extract. The immature persimmon fruit contains a lot of substances such as water, dietary fiber, protein, lipid, NFE (including sugar, tannic acid, etc.), and ash. Thus, a powdery composition that can be ingested can be obtained. In addition, a lipid metabolism improving agent and a bile acid excretion promoting agent, an anti-obesity agent and a defecation promoting agent comprising the immature persimmon fruit powder composition can be prepared.

  In addition, the method for drying immature fruits is not particularly limited. For example, a method of drying naturally by exposing it to sunlight for a long time, or using a dryer or the like (for example, an electric heater) is used. In addition, well-known equipment and means such as a method of forcibly drying can be used. In addition, the method of pulverizing the fruit including the skin and the seeds is pulverized by applying a strong force (pressure) such as using a pulverizer or the like (for example, a ball mill) or crushing the dried fruit. Etc. can also be used. As described above, the immature persimmon fruit powder composition of the present invention comprises water, dietary fiber, protein, lipid, NFE (including sugar, tannic acid, etc.), and ash. Such fruits are not limited to immature persimmon fruits, but are found to be mostly contained in common fruits (fruits) such as apples, pears, persimmons, and kiwis, although there are differences in content. It has been. Therefore, a composition obtained from immature fruit other than the above-mentioned immature fruit fruit as a raw material, as well as an immature fruit fruit powder composition, a lipid metabolism improving agent or a bile acid excretion promoter, and further an anti-obesity agent and faecal acceleration The agent may be used alone for preparation or may be used in admixture with an immature persimmon fruit powder composition.

  Therefore, according to the immature persimmon fruit powder composition of the present invention, it is possible to effectively utilize the harvested young fruit that has been conventionally disposed of, and the immature persimmon fruit that has been harvested two weeks or more before harvesting. An immature persimmon fruit powder composition containing water, dietary fiber, protein, lipid, NFE (including sugar, tannic acid, etc.), ash and the like can be obtained. Furthermore, a lipid metabolism improving agent having an effect of improving lipid metabolism comprising the immature persimmon fruit powder composition, a bile acid excretion promoting agent for promoting bile acid excretion, an anti-obesity agent for preventing obesity, and fecal A defecation promoter that promotes excretion can be prepared. As a result, it is relatively easy to secure immature fruits as raw materials, and not only provides a method for producing an immature persimmon fruit powder composition in which raw materials are stably supplied, but also discarded without being used. It is possible to effectively use the harvested fruit or the immature fruit harvested two weeks before harvesting, which contributes to the management of the fruit-growing farmers.

  Furthermore, the present invention is characterized by comprising a powder composition of mature or immature fruit containing at least water, dietary fiber, protein, lipid, NFE (including sugar, tannic acid, etc.), and ash. Bile acid excretion promoter ". Bile acid is known as an essential substance for absorption of lipids and fat-soluble vitamins in the intestinal tract, and contains water, dietary fiber, protein, lipid, NFE (including sugar, tannic acid, etc.) and ash of the present invention. By ingesting the immature fruit powder composition, bile acid excretion from the intestinal tract is promoted, so that bile acid synthesis is promoted in the body. The promotion of bile acid synthesis is useful as a preventive or therapeutic agent for arteriosclerosis, a prophylactic or therapeutic agent for obesity, and a prophylactic or therapeutic agent for gallstone formation because it reduces serum cholesterol. Here, NFE is a soluble nitrogen-free substance, and includes, for example, carbohydrates, polyphenols and the like.

  On the other hand, the lipid metabolism improving agent comprising the immature persimmon fruit powder composition of the present invention, or the bile acid excretion promoting agent, and further the antiobesity agent and the defecation facilitating agent production method, removes the buttocks from the immature persimmon fruit A step of cutting in small pieces in a state including the skin of the immature strawberry fruit from which the cocoon part has been removed, and drying the piece of strawberry fruit cut into pieces, containing in the strawberry fruit It comprises a drying process for removing the moisture to be performed and a powder process for pulverizing and powdering the dried dried strawberries. Here, for the immature strawberries used as raw materials, it is desirable to remove the buttocks in advance, but the skin and seeds can be dried and then pulverized and used as a part of the immature strawberries powder composition. .

  Therefore, according to the method for producing an immature persimmon fruit powder composition of the present invention, a pretreatment is performed in advance to remove the persimmon that becomes an obstacle in the drying and powdering steps, and the persimmon fruit is made into small pieces without peeling off the skin. Cut. The cut shape of the strawberry fruit should just be the shape which increases surface area, such as dice shape and strip shape, and is easy to dry. The dried persimmon fruits may be dried by exposing them to the sun and taking time to dry them, or using a warm air dryer. In the drying process, a “reduced pressure dryer” or a “vacuum dryer” can be used. The vacuum dryer can store a material to be dried in a hermetically sealed container and remove the moisture contained in the material to be dried by making the inside of the container a pressure lower than the atmosphere. The drying method is not particularly limited. However, if the heating temperature is too high, the active ingredient may be decomposed. Therefore, it is desirable to dry at an appropriate temperature.

  The dried immature fruit can be exemplified by a ball mill type pulverizer, an extrusion / shear type pulverizer, a stone mill type pulverizer, etc., but it is desirable to use a pulverizer that generates heat and does not decompose active ingredients. Furthermore, it is desirable to use a pulverizer that can pulverize the immature persimmon fruit powder composition of the present invention into a particle size suitable for purposes such as pharmaceutical use and health supplement use. In addition, in order to adjust the particle size of the lipid improving agent after pulverization by a pulverizer or the like, it may be sieved using a sieve having a predetermined mesh roughness.

  The lipid metabolism improving agent comprising the immature persimmon fruit powder composition obtained as described above, the bile acid excretion enhancer, and further the anti-obesity agent and the defecation enhancer are in a therapeutically effective amount in one or more It can be formulated with a pharmaceutically acceptable carrier (additive) and / or diluent. As described in detail below, the lipid metabolism improving agent composition comprising the immature persimmon fruit powder composition of the present invention, the bile acid excretion enhancer composition, and the anti-obesity agent and the defecation enhancer are as follows: For example, tablets, pills, powders, granules and the like can be exemplified for administration in solid form, including those particularly adapted.

  “Therapeutically effective amount” as used herein is an agent or composition effective to produce any desired therapeutic effect at a reasonable benefit / risk ratio applicable to any medical treatment. Means the amount. In the case of the immature persimmon fruit powder composition of the present invention, the daily intake for adults is 0.1 g to 50 g, preferably 1 g to 30 g.

  “Pharmaceutically acceptable” means, within the scope of good medical judgment, no problems or complications such as excessive toxicity, irritation, allergic reaction, etc., commensurate with a reasonable benefit / risk ratio. In addition, it is used to refer to compounds, materials, compositions, and / or dosage forms suitable for use in contact with human and animal tissues.

  “Pharmaceutically acceptable carrier” as used herein refers to transporting the lipid metabolism improving agent of the present invention from one organ or part of the body to another organ or part of the body. Means a pharmaceutically acceptable material, composition or excipient, such as a solid filler, diluent, excipient, solvent or encapsulating material involved. Each carrier must be “acceptable” in the sense of being compatible with the other ingredients of the dosage form and not injurious to the patient. Some examples of materials that can serve as pharmaceutically acceptable carriers include: (1) sugars such as lactose, glucose and sucrose; (2) starches such as corn starch and potato starch; (3) Cellulose and its derivatives such as sodium carboxymethylcellulose, ethylcellulose and cellulose acetate; (4) powdered tragacanth; (5) malt; (6) gelatin; (7) talc; (8) cocoa butter and excipients; (9) Oils such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; (10) glycols such as propylene glycol; (11) polyols such as glycerin, sorbitol, mannitol and polyethylene glycol ; (12) Oray Esters such as ethyl acrylate and ethyl laurate; (13) agar; (14) buffering agents such as magnesium hydroxide and aluminum hydroxide; (15) alginic acid; (16) pyrogen-free water; (17) isotonic sodium chloride. (18) Ringer solution; (19) Ethyl alcohol; (20) Phosphate buffer solution; and (21) Other non-toxic compatible substances used in drug formulations.

  Wetting agents such as sodium lauryl sulfate and magnesium stearate, emulsifiers and lubricants, and colorants, release agents, coatings, sweeteners, flavors and fragrances, preservatives and antioxidants are also lipid metabolism improving compositions. , And bile acid excretion promoters, as well as anti-obesity agents, and stool promoter compositions.

  Examples of pharmaceutically acceptable antioxidants include: (1) Water soluble antioxidants such as ascorbic acid, cysteine hydrochloride, sodium hydrogensulfate, sodium disulfite, sodium sulfite and the like; (2 ) Oil-soluble antioxidants such as ascorbyl palmitate, butylhydroxyanisole (BHA), butylhydroxytoluene (BHT), lecithin, propyl gallate, α-tocopherol and the like; and (3) citric acid, ethylenediaminetetraacetic acid (EDTA) ), Metal chelating agents such as sorbitol, tartaric acid, phosphoric acid and the like.

  The lipid metabolism improving agent comprising the immature persimmon fruit powder composition of the present invention, or the bile acid excretion enhancer, and the dosage forms of the anti-obesity agent and the defecation enhancer may be administered in a unit dosage form including oral administration. Well, it may be prepared by any method known in the pharmaceutical arts. The amount of immature persimmon fruit powder composition that can be combined with a carrier material to produce a single dosage form is effective as a lipid metabolism improving agent, or a bile acid excretion enhancer, as well as an anti-obesity agent and a defecation enhancer. It can be varied depending on the amount to be exerted and the particular mode of administration. The amount of immature strawberry fruit powder composition that can be combined with a carrier material to produce a single dosage form is generally that amount of immature strawberry fruit powder composition that produces a therapeutic effect. Generally, out of 100 percent, this amount ranges from about 1% to about 99%, preferably from about 5% to about 70%, most preferably from about 10% to about 30%. It is a lipid metabolism improving agent comprising a fruit powder composition, a bile acid excretion promoter, an anti-obesity agent and a defecation promoter.

  The methods of preparing these dosage forms or compositions comprise the step of mixing the immature persimmon fruit powder composition of the present invention with a carrier and optionally with one or more accessory ingredients. In general, dosage forms can be prepared by uniformly and intimately bringing into association the immature persimmon fruit powder composition of the present invention with a finely divided solid carrier and, if necessary, shaping the product.

  Suitable dosage forms of the present invention for oral administration may be in the form of capsules, cachets, pills, tablets, lozenges (with seasoned active ingredients, usually sucrose and gum arabic or tragacanth), powders, granules, A predetermined amount of the immature persimmon fruit powder composition of the present invention is included as an active ingredient. The immature persimmon fruit powder composition of the present invention may be administered as a bolus, electuary or paste.

  Solid dosage form (capsule, tablet, pill, dragee, powdered drug, granule, etc.) of lipid metabolism improving agent or bile acid excretion enhancer of the present invention for oral administration, as well as anti-obesity agent and defecation enhancer Is mixed with one or more pharmaceutically acceptable carriers such as sodium citrate or dicalcium phosphate and / or any of the following: (1) starch, lactose, sucrose, glucose, mannitol And / or fillers or extenders such as silicic acid; (2) binders such as carboxymethylcellulose, alginate, gelatin, polyvinylpyrrolidone, sucrose and / or gum arabic; (3) glycerol, etc. Moisturizer; (4) agar, calcium carbonate, potato or tapioca starch, alginic acid, a Certain silicates and disintegrants such as sodium carbonate; (5) dissolution retardants such as paraffin; (6) absorption enhancers such as quaternary ammonium compounds; (7) cetyl alcohol and glycerol monostearate (8) absorbents such as kaolin and bentonite clay; (9) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycol, sodium lauryl sulfate, and mixtures thereof; and (10) Colorant. In the case of capsules, tablets and pills, the drug composition may comprise a buffer. Similar types of solid compositions can also be used as fillers in soft and hard-filled gelatin capsules with excipients such as lactose or lactose and high molecular weight polyethylene glycols and the like.

  A tablet may be made by compression or molding, optionally with one or more accessory ingredients. Compressed tablets can be binders (eg, gelatin or hydroxypropylmethylcellulose), lubricants, inert diluents, preservatives, disintegrants (eg, sodium glycolate starch or crosslinked sodium carboxymethylcellulose), surfactants Alternatively, it can be prepared using a dispersant. Molded tablets can be made by molding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent.

  It is optional to make the lipid metabolism improving agent of the present invention, such as sugar-coated tablets, capsules, pills and granules, or bile acid excretion promoters, and further anti-obesity agents and defecation promoters into solid dosage forms such as tablets. Alternatively, it may be scored or prepared using coatings and shells such as enteric coatings well known in the drug dispensing arts. They use, for example, hydroxypropyl methylcellulose, other polymer matrices, liposomes and / or microspheres in various ratios to provide the desired release profile, and slow or controlled release of the internal active ingredient. May be formulated to provide. It may also be a composition that releases a lipid metabolism improving agent only in a certain part of the gastrointestinal tract or preferentially there, optionally in a delayed manner. Examples of embedding compositions that can be used are polymeric substances and waxes.

  The liquid dosage form for oral administration of the lipid metabolism improving agent comprising the immature persimmon fruit powder composition of the present invention, or the bile acid excretion enhancer, and further the anti-obesity agent and the defecation enhancer is pharmaceutically acceptable. There are resulting emulsions, microemulsions, solutions, suspensions, syrups and elixirs. Liquid dosage forms include, in addition to the immature persimmon fruit powder composition of the present invention, inert diluents commonly used in the art, such as water and other solvents, ethyl alcohol, isopropyl alcohol, ethyl carbonate, acetic acid Ethyl, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butadiene glycol, oil (especially cottonseed oil, peanut oil, corn oil, embryo oil, olive oil, castor oil and sesame oil), glycerol, tetrahydrofuryl alcohol, polyethylene glycol And solubilizers and emulsifiers, such as fatty acid esters of sorbitan, and mixtures thereof.

  In addition to inert diluents, oral compositions may include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, coloring, flavoring and preserving agents.

  Suspensions may be suspended in addition to the active compound, for example, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar and tragacanth, and mixtures thereof. A turbidity agent may be included.

  Lipid metabolism-improving agent comprising the immature persimmon fruit powder composition of the present invention, bile acid excretion enhancer, and further anti-obesity agent and defecation enhancer are removed as raw materials. Previously picked immature persimmon fruits can be used as raw materials. Compared with the method of purifying and utilizing the active ingredient, it can be produced at a low cost, and a synergistic effect with other ingredients is also expected. Furthermore, by ingesting a lipid metabolism improving agent comprising the immature persimmon fruit powder composition of the present invention, a bile acid excretion promoting agent, an anti-obesity agent and a defecation promoting agent, arteriosclerosis, myocardial infarction, obesity, etc. The onset can be suppressed. Furthermore, it is possible to suppress the occurrence of acne, pimples, rough skin, stiff shoulders and colorectal cancer due to occurrence of myocardial infarction and arteriosclerosis due to obesity and abnormal intestinal fermentation due to constipation. Furthermore, this invention can provide the health supplement which contains an immature persimmon fruit powder composition and has a lipid metabolism improving effect, a bile acid excretion promoting effect, an anti-obesity effect, or a defecation promoting effect.

  The immature persimmon fruit powder composition of the present invention, the lipid metabolism improving agent containing the immature persimmon fruit powder composition, the bile acid excretion promoter, the anti-obesity agent and the defecation promoter, and the production method thereof are as follows: However, the present invention is not limited to the following examples.

(1) Production of persimmon fruit powder Using the following six kinds of persimmon fruit, an attempt was made to produce an immature persimmon fruit powder composition as a raw material for a lipid metabolism improving agent and a bile acid excretion promoter.

Sample 1: Fuyu strawberry juvenile Sample 4: Bee pod juvenile Sample 2: Fuyu cocoon immature fruit Sample 5: Bee pod immature fruit Sample 3: Fuyu cocoon mature fruit Sample 6: Six kinds of strawberry fruit Was used. Here, the young fruit is an immature persimmon fruit harvested relatively early after flowering, and the immature fruit is an immature persimmon fruit harvested at least two weeks before harvest. As the mature fruit, a mature persimmon fruit that is usually eaten was used.

  Each of the above 6 types of cocoons was cut into dice using 1 kg each and dried with a vacuum dryer. The drying conditions of the vacuum dryer are such that the temperature of the vacuum dryer is 30 ° C. to 30 ° C. so that the active ingredients that exhibit the lipid metabolism improving action and the bile acid synthesis promoting action contained in the persimmon fruit are not destroyed. Minutes, 35 ° C.-2 hours, 37 ° C.-2 hours, 38 ° C.-2 hours, 39 ° C.-2 hours, 40 ° C.-4-6 hours. Over about 14 hours and 30 minutes, 150 g to 200 g of a dried product having a moisture content of about 5.5 to 10% was obtained.

In order to adjust the particle size, each dried persimmon fruit is adjusted to a particle size of 125 to 149 μm using a 100 to 120 mesh sieve in the pulverization step. Analyzed. Moreover, in order to see the influence by eating, it mixed with the animal feed and used for the intake test.
(2) Analysis of persimmon fruit powder Table 1 shows the results of component analysis of the persimmon fruit powder composition obtained above. The sample numbers in the table are the same as the sample numbers in (1) above. Each component content in the table is%. However, NFE is calculated as 100− (protein + lipid + dietary fiber + water + ash), and sugars, polyphenols and the like are contained therein. Moreover, tannic acid is a ratio (%) contained in 100 grams of the strawberry fruit powder composition measured separately. From this result, when dried and powdered strawberries made from the immature strawberries of the present invention, at least water 5.5 to 10%, dietary fibers 35 to 52%, proteins 3.0 to 8.0%, lipids It was found to contain 0.6-1.2%, NFE 53.4-23.8%, and ash 2.5-5.0%. As shown in the right column, the tannic acid content was 1.6 to 3.2% in mature fruits, but 3.3 to 15% in young and immature fruits.

ＮＦＥ＝１００−（水分＋蛋白質＋脂質＋食物繊維＋灰分）を示す。

NFE = 100− (water + protein + lipid + dietary fiber + ash).

  *: Tannic acid is a ratio (%) contained in 100 grams of separately measured strawberry fruit powder.

(3) Formulation Example A strawberry fruit powder composition obtained in Example 1 (1) is blended in the proportions shown below to improve a lipid metabolism improver, a bile acid excretion promoter, an antiobesity agent, and defecation. Tablets for the accelerator were prepared.

D-mannitol ………………………… 300 parts by weight Lactose …………………………………… 300 parts by weight Crystalline cellulose ………………………… 60 parts by weight Hydroxypropyl cellulose: 30 parts by weight Persimmon fruit powder: 310 parts by weight Composition ratio of immature persimmon fruit powder composition in the above formulation example, and pharmaceutically acceptable addition The drug should be changed as appropriate for the patient to be applied, such as the target disease to be applied, severity of injury, etc., and lipid metabolism improving agents with different contents of immature persimmon fruit powder composition, bile acid excretion promoters, and Obesity agents and defecation promoters can be adjusted.

  In Example 2, samples used for pharmacological effect tests such as lipid metabolism improving action or bile acid excretion promoting action comprising immature persimmon fruit powder composition were obtained by the same operation as in Example 1, and the following four kinds The strawberry fruit powder was used. A feed obtained by adding 10% of each sample to an obesity-inducing feed was used as a test feed, and only the non-added obesity-inducing feed was freely fed to the control group.

Sample 1: immature fruit
Specimen 2: Bee pod immature fruit Specimen 3: Fuyu strawberry mature fruit Sample 4: Bee strawberry ripened fruit (1) Effect of ingestion of immature strawberry fruit powder composition One group of mice (C57B / 6J, male, 6 weeks old) Four animals were fed a test feed (boiled fruit powder) and a control feed, and the consumption of the feed and the body weight were measured weekly. FIG. 1 is a graph showing the change in the weight of a mouse for each lap. FIG. 2 (a) is a graph showing the weight gain of the mice after 14 weeks of each feed, and FIG. 2 (b) is a graph showing the average intake of the feed taken by the mice.

As shown in FIG. 1, the body weight of the mice tended to increase every week. Except for Sample 2 using immature bee candy, there was no significant difference from the control group. Sample 2 showed a slight difference from the control group from the start of the experiment, and the difference between the control group and the control group gradually increased after 10 weeks from the start of the experiment. Finally, as shown in FIG. 2 (a), it was recognized that the weight gain was suppressed by about 10%. As shown in FIG. 2 (b), there was no difference in the amount of intake of the sample per day. From this result, it was confirmed that the sample 2 (Himiya immature fruit) has an effect of suppressing weight gain.
(2) Lipid metabolism improving effect of persimmon fruit powder 14 weeks after the start of the experiment of (1) above, the mouse was sacrificed in a carbon dioxide container, blood was collected, the mouse was dissected, and the epididymal visceral fat The liver and kidney were collected and the weight of each organ was measured. Plasma is separated from the collected blood, and using various measurement kits (blood glucose level; glucose; II test; total cholesterol; cholesterol E test; LDL; L type LDL-C; free fatty acid; NEFA C-test) Measurement of fat metabolism markers was performed.

  FIG. 3A is a graph showing the epididymal visceral fat weight, FIG. 3B is the liver weight, and FIG. 3C is the kidney weight. FIG. 4 is a graph showing the measurement results of (a) glucose concentration, (b) total cholesterol amount, and (c) triglyceride in plasma. FIG. 5 shows the measurement results of (a) LDL and (b) free fatty acids.

  By ingesting the lipid metabolism-improving agent of the present invention, as shown in FIG. 3 (a), the weight of visceral fat around the epididymis was changed to Sample 1 (Furuyu 柿 immature fruit) and Sample 2 (Hachiya 柿 immature fruit). ) Significantly lower than the control group. In addition, as shown in FIG. 3B, the liver weight was lower in any group of Samples 1 to 4 than in the control group. As shown in FIG. 3 (c), there was no difference in kidney weight between the four sample groups and the control group.

  As shown in FIG. 4 (a), the blood glucose level in blood plasma of mice and lipid metabolism markers are not different from the control group in the blood glucose level (blood glucose concentration). It is considered that it has no effect. As shown in FIG. 4 (b), for total cholesterol, FIG. 4 (c) triglyceride, and FIG. 5 (a) LDL, sample 1 (Fuyu 柿 i immature fruit) and sample 2 using immature koji fruits as raw materials A remarkable inhibitory effect was observed in (immature fruit of bee melon). The measured value of triglyceride in FIG. 4 (c) is 20% in the sample 1 and sample 2 groups using immature persimmon fruit as compared to the control group and the sample 3 and sample 4 intake group using the mature persimmon fruit as raw materials. Even lower results were obtained. Moreover, the free fatty acid of FIG. 5 (b) showed a value about 10 to 20% lower in each of the samples 1 to 4 intake group than the control group.

As mentioned above, by ingesting lipid metabolism improvers (sample 1 and sample 2) made from immature fruits, the total cholesterol, triglycerides, LDL, and free fatty acids are significantly lower than the control group It was confirmed that it has an excellent lipid metabolism improving effect.
(3) Effect of persimmon fruit powder on bile acid synthesis system Cholesterol in the body is catabolized to bile acid by the rate-limiting enzyme CYP7A1 (cholesterol 7α-hydroxylase) present in the liver and excreted in the duodenum. FIG. 6 shows the expression ratio of CYP7A1 in the liver after ingestion of feed containing strawberry fruit powder. As shown in FIG. 6, the expression of the bile acid synthesis rate-determining enzyme CYP7A1 in the liver was increased in the sample 1 and sample 2 feeding groups using immature fruits as a raw material, as compared to the control group.

A substance that promotes the expression of CYP7A1 (cholesterol 7α-hydroxylase) gene promotes cholesterol metabolism and is useful for prevention or treatment of arteriosclerosis, obesity, or gallstone formation. The immature persimmon fruit powder composition can be used for promoting cholesterol metabolism and the like.
(4) Bile acid excretion-promoting action One group of 5 mice (DBA / 2, male, 6-week-old) test feed (2%, 5%, 10% added group of bee melon immature fruit powder), and none The added control feed was given, and the amount of bile acids in feces was measured after 9 weeks. Cholestyramine used as a target drug is a hyperlipidemia-improving agent that has the effect of promoting the excretion of bile acids in the stool. It is known that the action of cholestyramine leads to the promotion of bile acid excretion in the feces → promotion of bile acid synthesis in the liver → reduction of blood cholesterol.

As shown in FIG. 7, the amount of bile acid excreted in the stool of mice fed with 2%, 5%, and 10% mixed bee strawberry immature fruit powder increased in a dose-dependent manner. The bile acid excretion promoting effect of the group of 5% bee strawberry immature fruit powder mixed with the feed is substantially the same as the group of 0.5% of cholestyramine having a known bile acid excretion promoting effect. Had. As a result, it was found that bee strawberry immature fruit powder has an effect of promoting the excretion of bile acids.
(5) Anti-obesity effect of persimmon fruit powder Specifically, 10% of dry powder prepared from young fruit, immature fruit and mature fruit of bee persimmon was added to the diet for obesity induction, and mice (ICR strain, male , 6 weeks old, 7 animals per group) and allowed to ingest for 7 weeks. The body weight after 7 weeks was measured, the amount of energy consumed was calculated from the amount of food ingested during that time, and the amount of weight gain per energy consumed was calculated and shown in FIG.

  Compared to the control group to which no koji powder was added, the effect of suppressing weight gain was clearly confirmed by adding the dried dry fruit powder (Sample 1) and the dried immature fruit powder (Sample 2). There was also a tendency for weight gain to be suppressed in the group to which the mature fruit dry powder (sample 3) was added, but there was no significant difference from the control group.

(6) Defecation promoting action of persimmon fruit powder Specifically, 10% of dry powder prepared from young fruit, immature fruit and mature fruit of bee cocoon was added to the diet for obesity induction, and mice (ICR strain, male , 6 weeks old, 7 animals per group) and allowed to ingest for 6 weeks. FIG. 9 shows the results of collecting feces excreted once a week during the breeding period for two days, drying and measuring the weight.

  The amount of feces excreted was clearly increased by adding young dried fruit powder (Sample 1) and immature fruit dried powder (Sample 2) compared to the control group to which no koji powder was added. Compared to the above, the dry fruit powder (sample 1) more than doubled, the immature fruit dry powder (sample 2) about twice, and the mature fruit dry powder (sample 3) about 1.3 times.

  As described above, according to the method for producing an anti-obesity and defecation promoter of the present embodiment and the anti-obesity and defecation promoter produced by the production method, a feed containing immature koji fruit powder is fed to a mouse. As a result, it was confirmed that weight gain was suppressed and fecal excretion was remarkably increased. From this result, it was revealed that the immature persimmon fruit powder composition has anti-obesity and defecation promoting effects.

Manufacture of health supplements-Biscuit A pinch of salt was added to 100 g of unsalted butter softened at room temperature to make a cream, and 70 g of sugar was divided into two portions and mixed. Two egg yolks were added while mixing one egg at a time, 120 g of weak flour and 30 g of immature strawberries powder were added thereto, and the dough was prepared by mixing well so that the whole blended. The dough was punched out with a suitable mold and baked in an oven at 180 ° C. for about 13 minutes to prepare a biscuit to be a health supplement. 30 biscuits containing 1 gram of immature persimmon fruit powder composition per biscuits were obtained.

・ Bread 15g butter, 2 tablespoons sugar, 1 teaspoon salt, 15g immature persimmon fruit powder, 1 tablespoon skim milk, 180cc water, knead well, add 1 teaspoon dry yeast and knead again. Bread containing immature persimmon fruit powder could be prepared by baking for 35 minutes at 170-190 ° C. after fermentation for hours.
In this way, by adding the immature koji fruit powder composition to foods such as biscuits and bread, various useful components contained in the immature koji fruit powder composition can be ingested without resistance. As its effects, lipid metabolism improvement effect, bile acid excretion promoting effect, anti-obesity effect and defecation promoting effect are expected, and a highly safe health supplement food material can be provided.

  The immature persimmon fruit powder composition of the present invention has a lipid metabolism improving action and a bile acid excretion promoting action, and further an anti-obesity action and a defecation promoting action, so that lipid metabolism is improved and bile acid excretion is further promoted. It can be used for suppression and prevention, or prevention and elimination of constipation. In addition to the biscuits and breads described above, various health supplements prepared by mixing immature persimmon fruit powder composition, for example, as immature persimmon fruit powder composition can be mixed into various foods as kneaded or liquid By doing so, health supplements that contribute to health can be provided.

It is a graph which shows the weight change for every week of a mouse | mouth. It is a graph which shows the increase amount of a mouse | mouth body weight, and (b) feed consumption of a mouse | mouth. It is a graph which shows each comparison of (a) epididymal visceral fat weight, (b) liver weight, and (c) kidney weight. It is a graph which shows the comparison of each measured value of (a) glucose concentration in plasma, (b) total cholesterol amount, and (c) triglyceride. It is a graph which shows the comparison of each measured value of (a) LDL and (b) free fatty acid. It is a graph which shows the expression rate of the bile acid synthesis type | system | group rate-limiting enzyme CYP7A1 in a liver. It is a graph which shows the amount of bile acid excretion in the stool of the mouse | mouth which ingested bee candy immature fruit powder. It is a graph which shows the amount of body weight increase with respect to the energy intake of the mouse | mouth which ingested bee candy immature fruit powder. It is a graph which shows the amount of defecation of the mouse | mouth which ingested bee candy immature fruit powder.

Claims (8)

  The raw material is immature persimmon fruit (referred to persimmon fruit picked between flowering and 2 weeks before maturity), and the moisture content of the dried and powdered composition of persimmon fruit is 5.5-10%, tannic acid is The immature persimmon fruit powder composition characterized by containing 3.3 to 15%.   Using immature persimmon fruit as a raw material, the persimmon fruit is dried and powdered, and the water content is 5.5-10%, dietary fiber 35-52%, protein 3.0-8.0%, lipid 0.6-1.2. %, NFE 53.4 to 23.8% (including saccharides, tannic acid and the like), and ash content 2.5 to 5.0%, .   A lipid metabolism improving agent comprising the immature persimmon fruit powder composition according to any one of claims 1 and 2.   A bile acid excretion promoter comprising the immature persimmon fruit powder composition according to any one of claims 1 and 2.     An anti-obesity agent comprising the immature persimmon fruit powder composition according to any one of claims 1 and 2.   A defecation promoter comprising the immature persimmon fruit powder composition according to any one of claims 1 and 2.     A health supplement comprising the immature persimmon fruit powder composition according to any one of claims 1 and 2. Removing the buttocks from the immature strawberries;
A cutting step of cutting the immature strawberry fruit from which the heel has been removed into a small piece in a state including a skin;
Drying the immature persimmon fruit pieces cut into small pieces, and removing the water contained in the immature persimmon fruit pieces;
It consists of a pulverization process that pulverizes and pulverizes the dried immature fruit pieces,
A method for producing an immature persimmon fruit powder composition, wherein the powder composition contains 5.5 to 10% moisture and 3.3 to 15% tannic acid.

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